Fibroblasts adult sheep cell derivation of stem cells from cloned human embryos. Medical search. Frequent questions

Embryonic stem cells (ESCs) are pluripotent stem cells derived from the inner cell mass of a blastocyst, an early-stage pre-implantation embryo. (wikipedia.org)
Pluripotent stem cells have shown promise in treating a number of varying conditions, including but not limited to: spinal cord injuries, age related macular degeneration, diabetes, neurodegenerative disorders (such as Parkinson's disease), AIDS, etc. (wikipedia.org)
But what is not getting such wide reporting is the use of pluripotent stem cells (as well as many other types of cells and genetic engineering techniques) for reproductive purposes . (lifeissues.net)
In 2006, Kazutoshi Takahashi and Shinya Yamanaka reprogrammed mice fibroblast cells, which can produce only other fibroblast cells, to become pluripotent stem cells, which have the capacity to produce many different types of cells. (asu.edu)
They called the pluripotent stem cells that they produced induced pluripotent stem cells (iPSCs) because they had induced the adult cells, called differentiated cells, to become pluripotent stem cells through genetic manipulation. (asu.edu)
The emergence and development of induced pluripotent stem cells (iPSCs) provides an approach to understand the regulatory mechanisms of cell pluripotency and demonstrates the great potential of iPSCs in disease modeling. (bvsalud.org)

These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. (biomedcentral.com)
Developmental defects, including abnormalities in cloned fetuses and placentas, in addition to high rates of pregnancy loss and neonatal death have been encountered by every research team studying somatic cloning. (biomedcentral.com)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
Many of these developmental abnormalities are common to human development. (edu.au)

Researchers are currently focusing heavily on the therapeutic potential of embryonic stem cells, with clinical use being the goal for many laboratories. (wikipedia.org)
Embryonic stem cells (ESCs), derived from the blastocyst stage of early mammalian embryos, are distinguished by their ability to differentiate into any embryonic cell type and by their ability to self-renew. (wikipedia.org)
Embryonic stem cells of the inner cell mass are pluripotent, meaning they are able to differentiate to generate primitive ectoderm, which ultimately differentiates during gastrulation into all derivatives of the three primary germ layers: ectoderm, endoderm, and mesoderm. (wikipedia.org)
Pluripotency distinguishes embryonic stem cells from adult stem cells, which are multipotent and can only produce a limited number of cell types. (wikipedia.org)
Under defined conditions, embryonic stem cells are capable of self-renewing indefinitely in an undifferentiated state. (wikipedia.org)
Pluripotency factors Oct4 and Nanog play a role in transcriptionally regulating the embryonic stem cell cycle. (wikipedia.org)
Due to their plasticity and potentially unlimited capacity for self-renewal, embryonic stem cell therapies have been proposed for regenerative medicine and tissue replacement after injury or disease. (wikipedia.org)
In addition to their potential in regenerative medicine, embryonic stem cells provide a possible alternative source of tissue/organs which serves as a possible solution to the donor shortage dilemma. (wikipedia.org)
According to a 2002 article in PNAS, "Human embryonic stem cells have the potential to differentiate into various cell types, and, thus, may be useful as a source of cells for transplantation or tissue engineering. (wikipedia.org)
A minority of mouse embryonic stem cells (ESCs) display totipotent features resembling 2-cell stage embryos and are known as 2-cell-like (2C-like) cells. (bvsalud.org)
Independently of the gestational periods, the qualitative evaluation showed the initial wash-in phase from the first appearance of the uterine artery to the rapid distribution in embryonic vesicles or placenta to the progressive washout, whilst there was no enhancement of either embryos or fetuses in any bitch. (edu.au)

Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. (biomedcentral.com)

Human embryos reach the blastocyst stage 4-5 days post fertilization, at which time they consist of 50-150 cells. (wikipedia.org)
Isolating the inner cell mass (embryoblast) using immunosurgery results in destruction of the blastocyst, a process which raises ethical issues, including whether or not embryos at the pre-implantation stage have the same moral considerations as embryos in the post-implantation stage of development. (wikipedia.org)
The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
The blastocyst can then be transferred to a recipient (h) and cloned animals are born after completion of gestation (i). (biomedcentral.com)
In this regard, emerging technologies of chimeric human organ production via blastocyst complementation (BC) holds great promise. (frontiersin.org)

Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)
A matured oocyte (c) is then enucleated (d) and a donor cell is transferred into the enucleated oocyte (e). (biomedcentral.com)

Aside from these uses, ESCs can also be used for research on early human development, certain genetic disease, and in vitro toxicology testing. (wikipedia.org)
Cells are collected from donor (a) and cultured in vitro (b). (biomedcentral.com)
Moreover, the knockdown of circ_0082319 might repress HCC cell proliferation, invasion, angiogenesis, and induce apoptosis in vitro. (bvsalud.org)
Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs [3] "Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock and for the treatment of human infertility. (edu.au)
Cells grown in vitro from neoplastic tissue. (lookformedical.com)

Malignant lymphoma composed of large B lymphoid cells whose nuclear size can exceed normal macrophage nuclei, or more than twice the size of a normal lymphocyte. (lookformedical.com)

Because cattle are a species widely used for nuclear transfer studies, and more laboratories have succeeded in cloning cattle than any other specie, this review will be focused on somatic cell cloning of cattle. (biomedcentral.com)
We also demonstrate that Nelfa overexpression significantly enhances somatic cell reprogramming efficiency. (bvsalud.org)

In this study, we derived skin fibroblasts from a patient with acute myelitis (P-HAF) and then reprogrammed P-HAF cells to iPSCs using eight exogenous factors (namely, OCT4, SOX2, c-MYC, KLF4, NANOG, LIN28, RARG, and LRH1). (bvsalud.org)

In particular, scientific developments in areas such as iPS cells open new possibilities of research and, at mid term, of therapeutic applications, but they also bring new ethical challenges and problems requiring further reflection and debate. (lifeissues.net)
HuR-mediated circ_0082319 might accelerate HCC cell proliferation, invasion, angiogenesis, and suppress apoptosis by the miR-505-3p/PTK2 axis, hinting at a promising therapeutic target for HCC treatment. (bvsalud.org)

Although the efficiency of nuclear transfer has been dramatically improved from the initial success rate of one live clone born from 277 embryo transfers [ 1 ], none of the aforementioned efforts abolished the common problems associated with nuclear transfer. (biomedcentral.com)

It is quite possible that the advances in human biology in the remainder of the twentieth century will be remembered as the most significant scientific achievement of the animal species known as Homo sapiens . (lifeissues.net)
However, somatic cloning has been inefficient in all species in which live clones have been produced. (biomedcentral.com)

But in order to become a part of medical history, parahuman reproduction and human genetic engineering must circumvent the recalcitrance of an antiquated culture. (lifeissues.net)
Recording and contextualizing the science of embryos, development, and reproduction. (asu.edu)

The cells are being studied to be used as clinical therapies, models of genetic disorders, and cellular/DNA repair. (wikipedia.org)

These germ layers generate each of the more than 220 cell types in the adult human body. (wikipedia.org)
Dynamics of male canine germ cell development [6] "Primordial germ cells (PGCs) are precursors of gametes that can generate new individuals throughout life in both males and females. (edu.au)

Rapid cell division allows the cells to quickly grow in number, but not size, which is important for early embryo development. (wikipedia.org)
increased public sensitivity and awareness together with the development of national regulations of governance of human cloning and embryo research in general. (lifeissues.net)

If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. (lookformedical.com)

Retinoblastoma proteins that inhibit the transcription factor E2F until the cell is ready to enter S phase are hyperphosphorylated and inactivated in ESCs, leading to continual expression of proliferation genes. (wikipedia.org)

When provided with the appropriate signals, ESCs initially form precursor cells that in subsequently differentiate into the desired cell types. (wikipedia.org)
ESCs divide very frequently due to a shortened G1 phase in their cell cycle. (wikipedia.org)
Despite this difference in the cell cycle when compared to ESCs grown in media containing serum these cells have similar pluripotent characteristics. (wikipedia.org)
Here, we report that the overexpression of negative elongation factor A (Nelfa), a maternally provided factor, enhances the conversion of ESCs into 2C-like cells in chemically defined conditions, while the deletion of endogenous Nelfa does not block this transition. (bvsalud.org)
Interestingly, we found that the co-overexpression of Nelfa and Bcl2 robustly activates the 2C-like state in ESCs and endows the cells with dual cell fate potential. (bvsalud.org)

Various strategies have been employed to modify donor cells and the nuclear transfer procedure in attempts to improve the efficiency of nuclear transfer. (biomedcentral.com)
Most of these efforts are focused on donor cells. (biomedcentral.com)
However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)

One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). (lookformedical.com)

A philosophy of reason will define a human being as one which demonstrates self-awareness volition and rationality. (lifeissues.net)

Currently, the efficiency for nuclear transfer is between 0-10%, i.e., 0-10 live births after transfer of 100 cloned embryos. (biomedcentral.com)

Computed tomography (CT) is widely used to evaluate morphological features and diagnose head disorders in adult dogs. (edu.au)

Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. (lookformedical.com)

The majority of mantle-cell lymphomas are associated with a t(11;14) translocation resulting in overexpression of the CYCLIN D1 gene (GENES, BCL-1). (lookformedical.com)

The use of various types of stem cells for research purposes to make disease "models" in the lab for regenerative medicine and for "therapies" to cure sick patients for diseases is constantly in the news. (lifeissues.net)
An in-depth analysis aiming at re-defining this terminology according to the new developments in human embryo research would be highly beneficial . (lifeissues.net)
3. National regulations of governance of human cloning and embryo research in general adopted so far confirm the convergence of views of the refusal to adopt legislation or guidelines permitting reproductive cloning , while they still show variations on the legitimacy of human cloning carried out as part of research agendas. (lifeissues.net)

In tissue engineering, the use of stem cells are known to be of importance. (wikipedia.org)
ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue. (thermofisher.com)
Any of a group of malignant tumors of lymphoid tissue that differ from HODGKIN DISEASE, being more heterogeneous with respect to malignant cell lineage, clinical course, prognosis, and therapy. (lookformedical.com)

In this review, we summarize the history of interspecies chimerism in various animal models to find hints for BC application and describe the challenges and prospects of utilizing BC for human organ generation. (frontiersin.org)

In his experiments, Bridges studied Drosophila, the common fruit fly, and by doing so showed that a process called nondisjunction caused chromosomes, under some circumstances, to fail to separate when forming sperm and egg cells. (asu.edu)
Schematic diagram of the somatic cloning process. (biomedcentral.com)

They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation. (lookformedical.com)

In 1980, Ernesto Ippolito and Ignacio Ponseti published their results on a histological study they performed on congenital club foot in human fetuses. (asu.edu)

Circ_0082319, PTK2, and HuR were highly expressed, and miR-505-3p was reduced in HCC samples and cell lines. (bvsalud.org)

Cyclin-dependent kinases such as CDK2 that promote cell cycle progression are overactive, in part due to downregulation of their inhibitors. (wikipedia.org)

The only common feature among these tumors is the absence of giant REED-STERNBERG CELLS, a characteristic of Hodgkin's disease. (lookformedical.com)

B-cell antigens are expressed on the immature cells that make up the tumor in virtually all cases of Burkitt lymphoma. (lookformedical.com)

In ancient history, humans used the term "chimera" to describe mythical creatures and hybrids. (frontiersin.org)

A form of non-Hodgkin lymphoma having a usually diffuse pattern with both small and medium lymphocytes and small cleaved cells. (lookformedical.com)

ZO-1 may be involved in signal transduction at cell-cell junctions. (thermofisher.com)

HuR expedited circ_0082319 expression in HCC cells. (bvsalud.org)

These changes result in accelerated cycles of cell division. (wikipedia.org)
It is likely that polyclonal B-cell lymphoproliferation in AIDS is a complex result of EBV infection, HIV antigenic stimulation, and T-cell-dependent HIV activation. (lookformedical.com)

Self-renewal conditions must prevent the cells from clumping and maintain an environment that supports an unspecialized state. (wikipedia.org)

Anatomy59

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