• Cancer-associated fibroblasts (CAFs) are one major type of component identified in the tumor microenvironment. (spandidos-publications.com)
  • In particular, cancer-associated fibroblasts (CAFs) participate in cancer progression and metastasis ( 10 , 11 ). (spandidos-publications.com)
  • Here, we combine spatial transcriptomics and scRNA-Seq datasets to define the transcriptome of tumor-proximal and tumor-distal cancer-associated fibroblasts (CAFs) and link this to clinical outcome. (elifesciences.org)
  • Cancer-associated fibroblasts (CAFs) are the most abundant constituents of the tumor microenvironment and have been linked to most hallmarks of cancer. (lu.se)
  • Cancer-associated fibroblasts actively compress cancer cells and modulate mechanotransduction. (bvsalud.org)
  • During tumor progression, cancer-associated fibroblasts (CAFs) accumulate in tumors and produce an excessive extracellular matrix (ECM), forming a capsule that enwraps cancer cells . (bvsalud.org)
  • Cancer-associated fibroblasts (CAF) stimulate tumor growth and metastasis. (lu.se)
  • Studies have focused on the genetic and epigenetic status of CAFs, since they are critical in tumor progression and differ phenotypically and functionally from normal fibroblasts. (spandidos-publications.com)
  • CAFs differ phenotypically and functionally from normal fibroblasts (NFs) ( 5 ). (spandidos-publications.com)
  • They compared CAFs from colon tumors to normal fibroblasts (NAFs) that were isolated from the same patient, in the adjacent normal tissue. (medindia.net)
  • A combination of markers was used to discriminate fibroblasts from other cell types to validate the purity of isolated cells and to discriminate NAFs from CAFs. (medindia.net)
  • Now, Gerald Crabtree and colleagues show that an miRNA might function similarly to "master regulators" in reprogramming cell fate, and, in particular, to sustain the conversion of fibroblasts directly into neurons. (alzforum.org)
  • Reprogramming of human fibroblasts into multipotent cells with a single ECM proteoglycan, fibromodulin. (ca.gov)
  • In this paper, we used an optimized protocol for adult skin fibroblast reprogramming based on RE1. (lu.se)
  • In this paper, we used an optimized protocol for adult skin fibroblast reprogramming based on RE1 Silencing Transcription Factor (REST) inhibitn together with a combination of GABAergic fate determinants to convert human adult skin fibroblasts into GABAergic neurons. (lu.se)
  • In lab models, Sen and the research team used their tissue nanotransfection (TNT) technology for tissue reprogramming to replenish vasculogenic fibroblasts in diabetic tissue. (iu.edu)
  • The main function of fibroblasts is to maintain the structural integrity of connective tissues by continuously secreting precursors of the extracellular matrix. (wikipedia.org)
  • The primary function of fibroblasts is the maintenance of structural integrity within the connective tissue. (news-medical.net)
  • Our results showed an increase in the expression of Cbfa1 and OCN in fibroblasts and osteoblasts exposed to fluoride and suggested that the osteogenic function of fibroblasts induced by fluoride could play an important role in the development of extraperiosteal ossification during skeletal fluorosis. (fluoridealert.org)
  • Receptors on the surface of fibroblasts also allow regulation of hematopoietic cells and provide a pathway for immune cells to regulate fibroblasts. (wikipedia.org)
  • Fibroblast growth factors and fibroblast growth factor receptors (FGFRs) play important roles in human axial and craniofacial skeletal development. (nih.gov)
  • The scRNA-seq analysis was used to characterize the dynamic changes associated with fibroblast differentiation and identified Gpx3 as a factor that might be involved in the regulation of myocardial fibrosis under cardiac pressure overload. (hindawi.com)
  • Subconjunctival fibrosis is mediated by the activities of human Tenon's fibroblasts (HTFs), which initiate myofibroblast differentiation upon stimulation by many fibrogenic factors. (molvis.org)
  • Local inflammation induces the differentiation of bronchial fibroblasts into α-smooth muscle actin (α-SMA) positive myofibroblasts and increases their longevity ( 5 ) through paracrine (for example TGFβ-dependent) and cell adhesion-dependent mechanisms ( 3 , 6 , 7 ). (spandidos-publications.com)
  • In response to tissue injury or stress, the resident cardiac fibroblasts undergo a transition to activated myofibroblasts, a highly synthetic cell type that induces the genes encoding smooth muscle α actin (ACTA2) and collagen. (kcl.ac.uk)
  • Although myofibroblasts have been considered the only phenotype of activated fibroblasts, distinct subsets of fibroblasts, namely inflammatory fibroblasts and myofibroblasts, have been reported in arthritis and pancreatic cancer. (kcl.ac.uk)
  • This study aimed to investigate the effect of nintedanib on the conversion of human Tenon's fibroblasts (HTFs) into myofibroblasts and reveal the molecular mechanisms involved. (molvis.org)
  • Accordingly, the activation of fibroblasts/myofibroblasts, which is characteristic for the asthmatic process, may facilitate lung cancer progression. (spandidos-publications.com)
  • Tumor-proximal fibroblasts comprise large populations of myofibroblasts, strongly expressed podoplanin, and were enriched for Wnt ligand signaling. (elifesciences.org)
  • For example, certain fibroblasts close to the tumour resemble the myofibroblasts present in healing wounds, while those at the periphery show signs of being involved in inflammation. (elifesciences.org)
  • Addgene: Selective conversion of fibroblasts into peripheral sensory neurons. (addgene.org)
  • A fibroblast is a type of biological cell that synthesizes the extracellular matrix and collagen, produces the structural framework (stroma) for animal tissues, and plays a critical role in wound healing. (wikipedia.org)
  • Fibroblasts secrete the precursors of all the components of the extracellular matrix, primarily the ground substance and a variety of fibers. (wikipedia.org)
  • Fibroblasts, like the tumor-associated host fibroblasts (TAF), play a crucial role in immune regulation through TAF-derived extracellular matrix (ECM) components and modulators. (wikipedia.org)
  • A fibroblast is a type of cell that is responsible for making the extracellular matrix and collagen. (news-medical.net)
  • In response to injury, myocardial fibroblasts show abnormal activation and secrete excessive extracellular matrix, resulting in chamber wall stiffness and diastolic dysfunction [ 5 ]. (hindawi.com)
  • Since heparan sulfate-proteoglycan is a major component of the extracellular matrix of skeletal muscle, factors that are bound to this proteoglycan, such as basic fibroblast growth factor (bFGF), are in a strategic position for neuronal signaling. (jneurosci.org)
  • Here, we provide evidence that exposure of human skin fibroblasts to a Radio Electric Asymmetric Conveyer (REAC), an innovative device delivering radio electric conveyed fields at a radiofrequency of 2.4 GHz, afforded remarkable commitment toward cardiac, neuronal, and skeletal muscle lineages. (nih.gov)
  • A team of researchers, led by Professor Kinya Otsu, showcase how a distinct subset of cardiac fibroblasts become activated by pressure overload and attract monocytes to the heart. (kcl.ac.uk)
  • They found that cardiac fibroblasts can produce CCL2 and name the cells inflammatory fibroblasts. (kcl.ac.uk)
  • They then examined the mRNA expression pattern at a single cell level using so-called single-cell RNA sequencing analysis and found a subset of cardiac fibroblasts highly expressed genes activated by the transcription factor NF-kB and CCL2 mRNA. (kcl.ac.uk)
  • In the mouse which did not express the NF-kB activator IKKb in cardiac fibroblasts, pressure overload induced less monocyte recruitment and did not induce heart failure. (kcl.ac.uk)
  • The adult heart contains many resident cardiac fibroblasts, which play important roles in maintaining tissue architecture. (kcl.ac.uk)
  • To conclude, we found that a subset of cardiac fibroblasts involved in cardiac inflammation under stress. (kcl.ac.uk)
  • The main purpose of this study was to characterize the fate of cardiac fibroblasts (CFs) and the dynamic gene expression patterns in a model of cardiac pressure overload using scRNA-seq analysis. (hindawi.com)
  • After ingestion of melanoma-derived exosomes during incubation, mouse embryonic fibroblasts cells emerged cellular invasiveness with the presentation of a marked increase in miR-21 expression. (dovepress.com)
  • Fibroblasts are originally derived from primitive mesenchyme and therefore display the filament protein vimentin, which acts as a marker of mesodermal origin. (news-medical.net)
  • Despite the well-known difficulty in converting adult fibroblasts into functional neurons in vitro, we could detect functional maturation in the induced neurons. (lu.se)
  • Denne metoden bidrar til å overvinne begrensninger i å studere menneskelig hematopoetisk utvikling ved å gi en enkel og tractable in vitro plattform basert på direkte celle omprogrammering. (jove.com)
  • The objective of this study was to determine the role of AREG in altering recovery from environmental dust exposure in a murine in vivo model and in vitro using cultured human and murine lung fibroblasts. (cdc.gov)
  • We hypothesize that tumor-derived exosomes endow the fibroblasts in tumor microenvironment with invasive phenotype to the benefit of tumor metastasis. (dovepress.com)
  • Our results suggest that tumor-derived exosomes may facilitate stromal fibroblasts an aggressive phenotype to equip the tumor progression. (dovepress.com)
  • The ability to reprogram fibroblasts into cardiomyocytes has many therapeutic implications. (ca.gov)
  • We report here a technique using a single molecule, recombinant human fibromodulin protein (FMOD), to reprogram human fibroblasts into multipotent cells. (ca.gov)
  • Fibroblasts and fibrocytes are two states of the same cells, the former being the activated state, the latter the less active state, concerned with maintenance and tissue metabolism. (wikipedia.org)
  • Though morphologically inconspicuous, ectopically transplanted fibroblasts can often retain positional memory of the location and tissue context where they had previously resided, at least over a few generations. (wikipedia.org)
  • Tissue damage stimulates fibrocytes and induces the production of fibroblasts. (wikipedia.org)
  • Besides their commonly known role as structural components, fibroblasts play a critical role in an immune response to a tissue injury. (wikipedia.org)
  • Conversely, fibroblasts sometimes undergo mesenchymal-epithelial transition (MET) to produce epithelia, a process that is seen in development, tissue repair and tumor growth. (news-medical.net)
  • MiR-21 promoted invasion of fibroblasts by down-regulation of tissue inhibitor of metalloproteinase 3 (TIMP3) expression and increasing of matrix metalloprotein (MMP) expression in fibroblast cells via melanoma-derived exosomes in a time-dependent manner. (dovepress.com)
  • Like other FGF family members, basic fibroblast growth factor possess broad mitogenic and cell survival activities, and is involved in a variety of biological processes, including embryonic development , cell growth , morphogenesis , tissue repair , tumor growth and invasion. (wikidoc.org)
  • Standing between a cancer cell in situ and the surrounding tissue of fibroblasts and extra-cellular matrix is the basement membrane, a thin sheet of fibers that normally cradles the cells above it. (medindia.net)
  • Established by George Todaro and Howard Green in 1962 from disaggregated Swiss mouse ( Mus musculus ) embryo tissue, the 3T3 cell line is a standard fibroblast cell line used in a wide spectrum of research and industrial biomedical applications. (fsu.edu)
  • When someone is injured, the body starts developing vasculogenic fibroblasts that make new blood vessels to heal tissue. (iu.edu)
  • Together with NeuroD2, Ascl1, and Myt1l, microRNAs (-9* and -124) convert human fibroblasts into functional neurons. (alzforum.org)
  • Using human colon cancer cells and primary human fibroblasts isolated from tumors and adjacent normal tissues, Alexandros Glentis and colleagues at the Institut Curie addressed the question of whether the cancer cells or the CAF cells were responsible for the breakdown of the basement membrane that leads to cancer progression. (medindia.net)
  • Through imaging the tumors and fibroblasts, the researchers then found the smoking gun of cancer-CAF cooperation--long protrusions like puppet-strings that the tumor cells used to communicate with the fibroblasts well before the cancer cells moved to breach the basement membrane. (medindia.net)
  • These data indicated that the interactions between lung cancer cells and HBFs in asthmatic bronchi may facilitate the colonization of lung tumors by fibroblasts. (spandidos-publications.com)
  • It was also shown to act on preosteoblasts - in the form of an increased proliferation - after binding to fibroblast growth factor receptor 1 and activating phosphoinositide 3-kinase . (wikidoc.org)
  • Fibroblasts can also migrate slowly over substratum as individual cells, again in contrast to epithelial cells. (wikipedia.org)
  • While epithelial cells form the lining of body structures, fibroblasts and related connective tissues sculpt the "bulk" of an organism. (wikipedia.org)
  • In certain situations, epithelial cells can give rise to fibroblasts, a process called epithelial-mesenchymal transition (EMT). (wikipedia.org)
  • As well as being present as fibroblasts, these cells exist in an alternative state, as fibrocytes. (news-medical.net)
  • Fibroblast is the term used to describe these cells when they are in an activated state. (news-medical.net)
  • In some cases, epithelial cells may also produce fibroblasts, a process which is referred to as epithelial-mesenchymal transition (EMT). (news-medical.net)
  • He reprogrammed mouse fibroblasts directly into primitive heart cells. (ca.gov)
  • Half of the cells in the heart are fibroblasts, so the ability to call upon this reservoir of cells already in the organ to become beating heart cells has tremendous promise for cardiac regeneration. (ca.gov)
  • Complimentary to this, fibroblasts exposed to fluid shear stress structurally rearranged pre-coated surface FN, but unstimulated and stretched cells did not. (nature.com)
  • The heart consists of many kinds of cells, such as heart cells, fibroblasts, and smooth muscle cells. (kcl.ac.uk)
  • Therefore, the aim of the present study was to identify a regulatory signaling pathway in cardiac inflammation based on the hypothesis that a distinct subset of inflammation-related activated fibroblasts plays crucial roles in the recruitment of inflammatory cells, especially monocytes/macrophages, and in the pathology of heart failure. (kcl.ac.uk)
  • Next steps for this research is to identify the marker for the inflammatory fibroblasts to isolate the cell population and examine the characteristics of the cells to regulate their activity. (kcl.ac.uk)
  • The invasion analysis showed that exosome-treated fibroblast cells had greater invasive capability as compared to untreated fibroblast cells, with the higher expressions of MMP2 and MMP9. (dovepress.com)
  • do cancer cells invade the basement membrane or do some fibroblasts help invading cancers? (medindia.net)
  • Using this technique, previous studies have shown that γ-aminobutyric acid (GABA) expressing interneurons can be generated from different cell sources, such as glia cells or fetal fibroblasts. (lu.se)
  • Swiss Mouse Embryo Fibroblast Cells with MitoTracker Red CMXRos, Alexa Fluor 488, and DAPI - A log phase culture of embryonic Swiss mouse fibroblast cells was stained with MitoTracker Red CMXRos, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular mitochondrial network, cytoskeletal actin filaments, and nuclei, respectively. (fsu.edu)
  • Mouse Embryo Fibroblast Cells with Texas Red-X, Alexa Fluor 488, and DAPI - In this section, a Swiss mouse embryo fibroblast cell is presented that was resident in a culture labeled with the fluorophore Texas Red-X conjugated to wheat germ agglutinin, a fluorescent lectin that selectively binds to sialic acid residues. (fsu.edu)
  • To estimate the effect of the asthmatic microenvironment on the invasiveness of lung cancer cells, the present study compared the behavior of human non‑small cell lung cancer A549 cells exposed to the signals from human bronchial fibroblasts (HBFs) derived from non‑asthmatic donors (NA HBFs) and from asthmatic patients (AS HBFs). (spandidos-publications.com)
  • However, the contribution of asthmatic bronchial fibroblasts in systemic dissemination of lung cancer cells remains unaddressed. (spandidos-publications.com)
  • Blood vessels are created by endothelial cells, but researchers found a different kind of cell, the vasculogenic fibroblast, can also make new blood vessels and achieve blood flow. (iu.edu)
  • Not only do these wrap pancreatic tumours in a dense, protective layer, they also foster complex relationships with the cancerous cells: some fibroblasts may fuel tumour growth, while other may help to contain its spread. (elifesciences.org)
  • Being able to specifically eliminate pro-cancer fibroblasts requires a better understanding of the factors that shape the role of these cells, and how to identify them. (elifesciences.org)
  • Cytotoxicity assays were performed by using cell culture medium containing L-929 line cells (mouse fibroblast). (bvsalud.org)
  • Genetic or pharmacologic inhibition of NOS1 reduced the growth of CXCL14-expressing fibroblasts along with their ability to promote tumor formation following coinjection with prostate or breast cancer cells. (lu.se)
  • Suppurative lesions become granulomatous, and histopathological examination of the granulomas shows a central necrotic, sometimes caseating, zone surrounded by a layer of epithelioid cells, multinucleated giant cells, and fibroblasts in a radial arrangement, typical of other granulomatous conditions such as tuberculosis and sarcoidosis. (cdc.gov)
  • MicroRNA-mediated conversion of human fibroblasts to neurons. (alzforum.org)
  • In addition to two papers from other groups published in Nature (1) and PNAS (2), this study adds more proof for the principle that a direct conversion of human neurons from fibroblasts is possible, which we proposed in our recent study (3). (alzforum.org)
  • The effect of basic fibroblast growth factor on motor neurons in the anterior horn of the injured spinal cord, and on the number of neuromuscular junctions in target organs, remains elusive. (medindia.net)
  • These findings, published in the Neural Regeneration Research (Vol. 8, No. 24, 2013), indicate that basic fibroblast growth factor can protect the endplate through attenuating the decreased expression of calcitonin gene related peptide and acetylcholinesterase in anterior horn motor neurons of the injured spinal cord. (medindia.net)
  • Nevertheless, the generation of neurons from adult human fibroblasts, an easily accessible cell source to obtain patient-derived neurons, has proved to be challenging due to the intrinsic blockade of neuronal commitment. (lu.se)
  • Moreover, we identified specific gene combinations that converted fibroblasts into neurons of a GABAergic intraneuronal fate. (lu.se)
  • Our results provide insight into the mechanisms underlying cardiac inflammation and fibroblast-mediated inflammatory responses that could be therapeutically targeted to treat heart failure. (kcl.ac.uk)
  • During the asthmatic process, epithelial damage is followed by the thickening of asthmatic bronchial walls, which is mechanistically linked to the local inflammation and the activation of bronchial fibroblasts ( 2 - 4 ). (spandidos-publications.com)
  • Each of these PNN-associated changes is reversed following a single intracerebroventricular (icv) injection of fibroblast growth factor 1 (FGF1) at a dose that induces sustained diabetes remission in male ZDF rats. (nature.com)
  • Central injection of fibroblast growth factor 1 induces sustained remission of diabetic hyperglycemia in rodents. (nature.com)
  • Here we show that micro-injection of normal human fibroblasts with an antibody directed against the p53-binding domain of mdm2 induces expression of p53-responsive genes, and furthermore results in p53-dependent growth arrest. (soton.ac.uk)
  • INDIANAPOLIS-Researchers from the Indiana Center for Regenerative Medicine and Engineering (ICRME) have identified a new type of cell in the human and mouse body called the vasculogenic fibroblast that provides critical insight into how new blood vessels can be made when needed for treatment. (iu.edu)
  • Stromal fibroblasts are a dominant but heterogenous population within the tumor microenvironment and therapeutic targeting of stromal subsets may have therapeutic utility. (elifesciences.org)
  • The plasticity and heterogeneity of fibroblasts in the tumor microenvironment of pancreatic ductal adenocarcinoma (PDAC) has emerged as a key factor in determining tumor growth and therapeutic response. (elifesciences.org)
  • Fibroblasts are associated with the metastasis of cancer depending on their cellular plasticity. (dovepress.com)
  • Conversely, fibroblasts in some situations may give rise to epithelia by undergoing a mesenchymal to epithelial transition (MET) and organizing into a condensed, polarized, laterally connected true epithelial sheet. (wikipedia.org)
  • Some researchers use the term fibroblast for both cellular states but the term "blast" usually refers to a stem cell or a cell's activated state. (news-medical.net)
  • also known as basic fibroblast growth factor (bFGF). (themmrf.org)
  • FGF2 , also known as basic fibroblast growth factor (bFGF) and FGF-β, is a growth factor and signaling protein encoded by the FGF2 gene . (wikidoc.org)
  • [3] [5] FGF2 binds to and exerts effects via specific fibroblast growth factor receptor (FGFR) proteins which themselves constitute a family of closely related molecules. (wikidoc.org)
  • Fibroblast Growth Factors (FGFs) comprise a family of at least eighteen structurally realted proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentation, angiogenesis, wound healing and tumorgenesis. (prospecbio.com)
  • Inactive fibroblasts (called fibrocytes) are smaller, spindle-shaped, and have a reduced amount of rough endoplasmic reticulum. (wikipedia.org)
  • The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. (nih.gov)
  • Specifically, elevated expression of HIF-1a and podoplanin predicted worse outcome while inflammatory gene expression correlated with increased survival, suggesting future interventions targeting proximal fibroblast populations to mitigate against PDAC. (elifesciences.org)
  • They mapped out the location of individual fibroblasts in the vicinity of the tumour and analysed their gene activity. (elifesciences.org)
  • Researchers observed vasculogenic fibroblasts in human patients. (iu.edu)
  • Basic fibroblast growth factor has been shown to interact with casein kinase 2, alpha 1 , [13] RPL6 [14] and ribosomal protein S19 . (wikidoc.org)
  • Jianlong Wang and team from Third Xiangya Hospital of Central South University established a rat model of spinal cord injury using a modified Allen's method, which was injected with basic fibroblast growth factor solution via the subarachnoid catheter. (medindia.net)
  • AREG treatment rescued the DE-induced inhibitory fibroblast effects. (cdc.gov)
  • Fibroblast Growth Factor 2 Levels in Patients with Major Depressive Disorder: A Meta-analysis. (nih.gov)
  • The cardiac fibroblast might therefore be a therapeutic target to treat patients with heart failure. (kcl.ac.uk)
  • Amphiregulin modulates murine lung recovery and fibroblast function following exposure to agricultur e organic dust. (cdc.gov)
  • In murine and human lung fibroblasts, DE induced the release of AREG and inflammatory cytokines. (cdc.gov)
  • Fibroblast recellularization of primary human lung mesenchymal matrix scaffolds and wound closure was inhibited by DE and enhanced with recombinant AREG alone. (cdc.gov)
  • The level of Cbfa1 mRNA in the fibroblasts was much higher at 48h in the group treated with 10.0mg/L F- than in the control group. (fluoridealert.org)
  • A slightly higher level of OCN mRNA in fluoride-treated fibroblasts was also found in the 1.0 and 20.0mg/L F- groups compared to the control group. (fluoridealert.org)
  • Bovine fibroblast growth factor 21 (FGF21) ELISA kit is Available at Gentaur Genprice with the fastest delivery. (joplink.net)
  • Collectively, these studies demonstrate that inhibition of AREG reduced, whereas AREG supplementation promoted, the airway inflammatory recovery response following environmental bioaerosol exposure, and AREG enhanced fibroblast function, suggesting that AREG could be targeted in agricultur al workers repetitively exposed to organic dust environments to potentially prevent and/ or reduce disease. (cdc.gov)
  • Fibroblast growth factor protein was first purified in 1975, but soon afterwards others using different conditions isolated basic FGF, Heparin-binding growth factor-2, and Endothelial cell growth factor-2. (wikidoc.org)
  • The hypothalamic arcuate nucleus-median eminence is a target for sustained diabetes remission induced by fibroblast growth factor 1. (nature.com)
  • Intracellular fibroblast growth factors (iFGFs) : novel regulators of neuronal excitability / Jeanne Nerbonne. (nih.gov)
  • Here the authors use innovative approaches to combine spatial profiling with single cell transcriptomics to define tumor-proximal populations of fibroblasts that predict clinical outcome. (elifesciences.org)
  • This may be due, in part, to a type of cell called fibroblasts. (elifesciences.org)
  • CIL:38651, Homo sapiens, fibroblast. (ucsd.edu)
  • Active fibroblasts can be recognized by their abundant rough endoplasmic reticulum. (wikipedia.org)