• Trumenba® (MenB-FHbp) is a recombinant protein-based vaccine targeting Neisseria meningitidis serogroup B. Trumenba is a sterile suspension composed of two recombinant lipidated factor H binding protein (fHBP) variants from N. meningitidis serogroup B, one from fHBP subfamily A and one from subfamily B (A05 and B01, respectively). (precisionvaccinations.com)
  • The Lancet Infectious Diseases reported on September 7, 2022, on the good immunogenicity, short-lived antibody persistence, and acceptable reactogenicity profile of meningococcal serogroup B-factor H binding protein vaccine (MenB-FHbp), which contains two factor-H-binding proteins, against serogroup B meningococcal disease in children aged 1-9 years. (precisionvaccinations.com)
  • The data were collected during two phase 2 studies in Australia and Europe, with 396 toddlers (age 12-23 months) and 400 older children (aged 2-9 years) randomly assigned to receive either MenB-FHbp (60 μg [toddler study only] or 120 μg) or a control vaccine (hepatitis A vaccine and saline) in a three-dose primary schedule (0, 2, and 6 months). (precisionvaccinations.com)
  • As these four primary strains are representative, they predict the ability of antibodies elicited by TRUMENBA to be active against diverse circulating strains. (worldpharmatoday.com)
  • Trumenba ® (rLP2086) is a bivalent fHbp-containing vaccine. (pubmlst.org)
  • Trumenba ® vaccine contains fHbp peptides 45 and 551. (pubmlst.org)
  • Description: Enzyme immunoassay for measurement of IgM antibodies against the 14 kD and OspC antigens of Borrelia burgdorferi in human serum, plasma and CSF. (peoriaobserver.com)
  • Data suggested that vaccination would control the outbreak because isolates expressed antigens that were closely related to the vaccine antigens (factor H-binding protein [fHbp] and neisserial heparin-binding antigen). (princeton.edu)
  • Overview of Immunization Immunity can be achieved Actively by using antigens (eg, vaccines, toxoids) Passively by using antibodies (eg, immune globulins, antitoxins) A toxoid is a bacterial toxin that has been modified. (msdmanuals.com)
  • Immunity to diseases can occur via active immunity (the immune system produces lymphocytes and/or antibodies following exposure to antigens) and passive immunity (immunity is acquired by the transfer of antibodies from a previously immunized person or someone who has recovered from a disease). (medscape.com)
  • Thus, the region of fHbp encompassing residues 25-59 in the N-terminal domain is important for eliciting antibodies that can cooperate with other anti-fHbp antibodies for cross-reactive bactericidal activity against strains expressing fHbp from different antigenic variant groups. (unimol.it)
  • Breadth of killing was mostly due to anti-fHbp antibodies, with some restriction according to ID type sequence differences. (ox.ac.uk)
  • Recombinant monoclonal antibody to Factor H binding protein. (absoluteantibody.com)
  • Mapping of epitopes recognized by functional monoclonal antibodies (mAbs) is essential for understanding the nature of immune responses and designing improved vaccines, therapeutics, and diagnostics. (rcsb.org)
  • Eculizumab is a humanized monoclonal antibody that inhibits the terminal complement protein C5 to prevent its cleavage into C5a and C5b. (clevelandclinic.org)
  • TY014 will be the first therapeutic monoclonal antibody specifically targeting the Yellow Fever Virus to enter clinical trials. (precisionvaccinations.com)
  • In an ELISA, this antibody shows concentration dependent binding to recombinant FHbp (ID 1), which is the variant group 1 antigen (PMID: 26106082). (absoluteantibody.com)
  • To investigate antigen-antibody interactions and assess the potential of the most common epitope mapping techniques, we generated a series of mAbs against factor H binding protein (fHbp), a key virulence factor and vaccine antigen of Neisseria meningitidis. (rcsb.org)
  • Collectively, these data show that linear epitope mapping techniques provide useful but incomplete descriptions of B-cell epitopes, indicating that increased efforts to fully characterize antigen-antibody interfaces are required to understand and design effective immunogens. (rcsb.org)
  • Meningococcal factor H-binding protein (fHbp) is a promising vaccine antigen. (unimol.it)
  • The coating antigen was 1 μg/mL non-lipidated recombinant hexa-Histidine-tagged fHbp ID1 [11]. (peptidecost.com)
  • The work reinforces that meningococcal NOMV with OE fHbp is a promising vaccine strategy, and provides a basis for rational selection of antigen sequence types for over-expression on NOMV. (ox.ac.uk)
  • FHbp protein is a bacterial surface lipoprotein that binds host (human) complement factor H (fH, gene CFH). (absoluteantibody.com)
  • The original Fab version of this antibody binds N. meningitidis fHbp protein with a very high binding affinity of Kd= 19pM. (absoluteantibody.com)
  • Functional Analysis of the Human Antibody Response to Meningococcal Factor H Binding Protein. (absoluteantibody.com)
  • Based on site-directed mutagenesis studies, the JAR 4 epitope could either be knocked out of a reactive variant 1 fHbp, or introduced into a non-reactive variant 3 protein. (unimol.it)
  • Contribution of factor H-Binding protein sequence to the cross-reactivity of meningococcal native outer membrane vesicle vaccines with over-expressed fHbp variant group 1. (ox.ac.uk)
  • The Antibodies Test Igg Igm reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (gentaurpriceonline.com)
  • Description: Qualitativeindirect ELISA kit for measuring Human platelet antibodies IgM (PA-IgM) in samples from serum, plasma. (gentaurpriceonline.com)
  • Description: The Candida albicans IgM Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgM antibodies against Candidaalbicans in serum and plasma. (putyourcolours.pl)
  • Description: Quantitativesandwich ELISA kit for measuring Human candida albicans (C.albicans) antibody (IgM) in samples from serum, plasma, cell culture supernates, tissue homogenates. (putyourcolours.pl)
  • Serum bactericidal antibody (SBA) activities were measured as described before [28]. (peptidecost.com)
  • Serum killing from mice immunised with rfHbp was generally less efficient and more restricted compared to NOMV, which induced antibodies that killed most meningococci tested, with decreased stringency for ID type differences. (ox.ac.uk)
  • METHODS: We conducted a seroprevalence survey among students to assess vaccination status and collect serum specimens to quantify titers of serum bactericidal antibodies (SBA) with an assay that included human complement (hSBA). (princeton.edu)
  • this can occur naturally via maternal-fetal transfer of antibodies, or it may be acquired through injection of serum from immune individuals. (medscape.com)
  • This antibody is broadly cross reactive and binds FHbp sequence variants from groups 1, 2, and 3. (absoluteantibody.com)
  • This antibody binds a surface exposed epitope on N. meningitidis FHbp, a virulence factor critical for surviving of the bacteria in the human host. (absoluteantibody.com)
  • Previous studies described three fHbp antigenic variant groups and identified amino acid residues between 100 and 255 as important targets of variant-specific bactericidal antibodies. (unimol.it)
  • We generated isogenic NOMV-producing meningococcal strains from an African serogroup W isolate, each over-expressing one of four fHbp variant group 1 sequences (ID 1, 5, 9, or 74), including those most common among invasive African meningococcal isolates. (ox.ac.uk)
  • The original antibody was generated from the peripheral blood plasmablast cells from three humans immunized with vaccines containing FHbp. (absoluteantibody.com)
  • Describes the generation and characterization of human anti-FHbp repertoire from three adults immunized with FHbp vaccines. (absoluteantibody.com)
  • The interaction of fHbp with the bactericidal mAb 12C1 was studied by various epitope mapping methods. (rcsb.org)
  • Although a 12-residue epitope in the C terminus of fHbp was identified by both Peptide Scanning and Phage Display Library screening, other approaches, such as hydrogen/deuterium exchange mass spectrometry (MS) and X-ray crystallography, showed that mAb 12C1 occupies an area of ∼1,000 Å(2) on fHbp, including >20 fHbp residues distributed on both N- and C-terminal domains. (rcsb.org)
  • We investigated residues affecting expression of an epitope recognized by a murine IgG2a anti-fHbp mAb, designated JAR 4, which cross-reacted with fHbps in variant group 1 or 2 (95% of strains), and elicited human complement-mediated, cooperative bactericidal activity with other non-bactericidal anti-fHbp mAbs with epitopes involving residues between 121 and 216. (unimol.it)
  • Flow cytometry was used to identify binding of this antibody to surface of live bacteria of N. meningitidis strains serogroup B strain H44/76, which expresses FHbp ID 1 (PMID: 26106082). (absoluteantibody.com)
  • When the person is then exposed to the bacterium, these antibodies together with other components of the immune system will be able to kill the bacteria and help protect against the disease. (europa.eu)
  • Two main studies showed that Bexsero was effective at stimulating an immune response to N. meningitidis group B. The studies measured the production of protective antibodies which are able to kill the bacteria. (europa.eu)
  • Fromfilamentous bacteriophage libraries containing random peptides thatwere recognized by JAR 4, we identified a consensus tripeptide,DHKthat matched residues 25-27 in the N-terminal domain of fHbp. (unimol.it)
  • NOMV over-expressing different fHbp IDs belonging to variant group 1 induce antibodies with fine specificities against fHbp, and ability to kill broadly meningococci expressing heterologous fHbp IDs. (ox.ac.uk)
  • We compared the proportion of vaccinated and unvaccinated participants who were seropositive for the outbreak strain and for one closely related reference strain (44/76-SL, which included fHbp) and one mismatched reference strain (5/99, which included neisserial adhesin A), both of which were used in vaccine development. (princeton.edu)
  • This antibody binds equally well to fHbp variants, including representative variants in variant groups 1, 2 or 3. (absoluteantibody.com)
  • Two phase 2b, randomized, multicenter studies were conducted (NCT02212457, NCT02946385) to assess the immunogenicity and safety of the MenABCWY vaccine as well as antibody persistence and response to a booster dose 2 years after the last vaccination, compared to 4CMenB vaccination. (bvsalud.org)
  • Two other groups of mice received 5 μg of GMMA from the Double KO (lpxL1, gna33 KO) OE fHbp mutant or 5 μg GMMA from the Triple KO mutant strain. (peptidecost.com)
  • Redirecting endogenous antibodies in the bloodstream to tumor cells utilizing artificial molecules is a promising strategy to set off anti-tumor immune responses. (kashbiotech.com)
  • Nevertheless, under our experimental conditions, no relationship between antibody cross-reactivity and variation fH-binding site sequence was identified. (ox.ac.uk)
  • Fc-ARMs are composed of an Fc-binding peptide and a concentrating on ligand , enabling the exploitation of endogenous antibodies via fixed affinity to the Fc area of antibodies, whose sequence is conserved in distinction to the Fab area. (kashbiotech.com)
  • Peptide sequence diversity can be analysed using the fhbp peptide locus. (pubmlst.org)
  • Native outer membrane vesicles with over-expressed fHbp (NOMV OE fHbp) have been shown to induce antibodies with broader functional activity than recombinant fHbp (rfHbp). (ox.ac.uk)
  • Nonetheless, present molecular designs solely allow the usage of a small fraction of endogenous antibodies, limiting the therapeutic potential. (kashbiotech.com)
  • We performed a pair-wise analysis of 48 surface-exposed amino acids involved in interacting with factor H, among 383 fHbp variant group 1 sequences. (ox.ac.uk)
  • Assess antibody response 1 to 2 months after completion of the series. (hiv.gov)
  • The signaling inhibitory antibody quickly induces ligand-independent receptor clustering and internalization via each caveolin and clathrin-mediated pathways. (kashbiotech.com)
  • La identificación de portadores del virus de la hepatitis B en donantes de sangre es imperativo para evitar la transmisión de la enfermedad a través de transfusiones sanguíneas. (kashbiotech.com)
  • Determinar si los donantes de sangre con resultados positivos en los marcadores serológicos HbsAg y anti-HBc eran portadores del ADN del virus de la hepatitis B. (kashbiotech.com)
  • Custom development of ELISAs for other species or antibody isotypes not listed in the catalog. (peoriaobserver.com)
  • The non-inhibitory antibody additionally effectively endocytoses through clathrin with out inducing receptor clustering however with slower lysosomal co-localization kinetics. (kashbiotech.com)
  • When a person is given the vaccine, the immune system recognises the parts of the bacterium contained in the vaccine as 'foreign' and makes antibodies against them. (europa.eu)
  • On June 27, 2019, eculizumab, or Soliris, became the first FDA approved treatment for anti-aquaporin-4 (AQP4) antibody positive neuromyelitis optica spectrum disorder (NMOSD) in adults. (clevelandclinic.org)
  • Eculizumab had previously been FDA approved for treatment of paroxysmal nocturnal hemoglobinuria (PNH), atypical hemolytic uremic syndrome (aHUS), and anti-acetylcholine receptor antibody positive generalized myasthenia gravis (gMG). (clevelandclinic.org)
  • Eculizumab was first studied in 14 AQP4 antibody positive neuromyelitis optica patients in an open-label trial. (clevelandclinic.org)
  • Q. How is eculizumab administered in AQP4 antibody positive NMOSD? (clevelandclinic.org)
  • While the PREVENT trial did not pharmacodynamics of eculizumab in APQ4 antibody positive NMOSD patients, pharmacodynamics of eculizumab has been studied in other disease states. (clevelandclinic.org)
  • Headache, nausea, and dizziness were the most common side effects reported in the open label trial of eculizumab in APQ4 antibody positive neuromyelitis optica patients. (clevelandclinic.org)
  • Control mice were immunised with 5 μg recombinant fHbp ID1 or aluminium hydroxide only. (peptidecost.com)