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Satellite Cells, Skeletal MuscleMyoD ProteinTranscription FactorsMyogeninPaired Box Transcription FactorsMyogenic Regulatory Factor 5PAX7 Transcription FactorTranscription, GeneticDNA, SatelliteMuscle DevelopmentPAX2 Transcription FactorMyogenic Regulatory FactorsSatellite Cells, PerineuronalDNA-Binding ProteinsB-Cell-Specific Activator ProteinPromoter Regions, GeneticGene Expression RegulationMuscle, SkeletalMolecular Sequence DataBase SequenceCell DifferentiationHomeodomain ProteinsGene Expression Regulation, DevelopmentalTrans-ActivatorsRepressor ProteinsMuscle ProteinsMyoblastsRNA, SatelliteRNA, MessengerRegenerationMusclesEye ProteinsMuscle Fibers, SkeletalNuclear ProteinsSp1 Transcription FactorMEF2 Transcription FactorsCell LineCells, CulturedPAX9 Transcription FactorHelix-Loop-Helix MotifsTranscriptional ActivationDNAAmino Acid SequenceSatellite CommunicationsProtein BindingBinding SitesBasic Helix-Loop-Helix Transcription FactorsSignal TransductionSatellite VirusesTransfectionTranscription Factor AP-1Enhancer Elements, GeneticForkhead Transcription FactorsCell NucleusCell LineageGene ExpressionCell ProliferationMutationReverse Transcriptase Polymerase Chain ReactionStem CellsMice, TransgenicMice, Inbred C57BLBasic-Leucine Zipper Transcription FactorsGenes, ReporterTranscription Factor AP-2Mice, KnockoutSequence Homology, Nucleic AcidChromatin ImmunoprecipitationKruppel-Like Transcription FactorsTranscription Factors, TFIIImmunohistochemistrySTAT3 Transcription FactorCardiotoxinsHeLa CellsIn Situ HybridizationYY1 Transcription FactorGene Expression ProfilingPhenotypeGATA4 Transcription FactorTranscription Factor TFIIDActivating Transcription Factor 3Cloning, MolecularMyostatinNFATC Transcription FactorsTranscription Initiation SiteElectrophoretic Mobility Shift AssaySp3 Transcription FactorRecombinant Fusion ProteinsNF-kappa BZinc FingersBasic Helix-Loop-Helix Leucine Zipper Transcription FactorsActivating Transcription Factor 2Transcription Factor TFIIBPlasmidsRegulatory Sequences, Nucleic AcidBlotting, WesternRNA Polymerase IIE2F1 Transcription FactorEmbryo, MammalianModels, Biological
Skeletal muscleMyoDDifferentiationGeneUndergoHepatocyte growthPromotesProteinStem cellsAdultNuclearImmunohistochemistryRegenerationGenesDifferentiateDevelopmentCytoplasmicCycleDefineStudiesResultsMultipleUndergoing myogenic differentiationMyogenesisProliferationLineagePAX3ProgenitorQuiescent satellite cellsMyoblastsMyofibersLimbProteinsStemMuscle fibersSignificantlyHumanSarcolemmaActivationIdentifyLeadsExpressionResponseActive
Skeletal muscle6
  • Satellite cells are precursors to skeletal muscle cells, able to give rise to satellite cells or differentiated skeletal muscle cells. (wikipedia.org)
  • In addition, IGF-1 expression in skeletal muscle extends the capacity to activate satellite cell proliferation (Charkravarthy, et al. (wikipedia.org)
  • The delayed growth of LBW piglets is mainly due to a slower development of skeletal muscle as the myogenic activity is usually impaired in these animals during the fetal and early postnatal period 3 , 4 . (nature.com)
  • Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)
  • Previous studies have shown that lncRNA-MEG3 promotes the differentiation of porcine skeletal muscle satellite cells (PSCs), but the regulatory mechanism of MEG3 interaction with target protein has not been well studied. (bvsalud.org)
  • Here, we examined the time-dependent expression patterns of multiple biomarkers associated with satellite cell fate, including the transcription factor paired box 7 (Pax7), myoblast determination protein (MyoD), myogenin, and insulin-like growth factor (IGF-1), using immunohistochemistry, western blotting, and quantitative real-time PCR in contused skeletal muscle. (bvsalud.org)
MyoD13
  • Greater activation also results in increased expression of myogenic basic helix-loop-helix transcription factors MyoD, myogenin, and MRF4 - all responsible for the induction of myocyte-specific genes. (wikipedia.org)
  • Furthermore, we have successfully predicted and validated that the transcription factor myogenic differentiation (MYOD) binds to the MEG3 core promoter though utilizing chromatin immunoprecipitation (CHIP) and luciferase reporter assays. (bvsalud.org)
  • The results indicated that MYOD acts as a transcription factor of MEG3 to promote MEG3 transcription. (bvsalud.org)
  • It is concluded that MYOD acts as a transcription factor to induce MEG3 expression. (bvsalud.org)
  • The bHLH transcription factor MyoD is a master regulator of myogenic differentiation, and its sustained expression in fibroblasts suffices to differentiate them into muscle cells. (bvsalud.org)
  • MyoD expression oscillates in activated muscle stem cells of developing, postnatal and adult muscle under various conditions: when the stem cells are dispersed in culture, when they remain associated with single muscle fibers, or when they reside in muscle biopsies. (bvsalud.org)
  • Unstable MyoD oscillations and long periods of sustained MyoD expression are observed when stem cells undergo myogenic differentiation. (bvsalud.org)
  • The oscillatory expression of MyoD is driven by the oscillatory expression of the bHLH transcription factor Hes1 that periodically represses MyoD. (bvsalud.org)
  • Thus, oscillations of MyoD and Hes1 control the balance between the proliferation and differentiation of muscle stem cells. (bvsalud.org)
  • Here, we describe time-lapse imaging methods using luciferase reporters, which can monitor dynamic MyoD gene expression in myogenic cells. (bvsalud.org)
  • Morphometrically, the data obtained from the numbers of Pax7 + , MyoD + , and myogenin + cells were highly correlated with the wound age. (bvsalud.org)
  • Pax7, MyoD, myogenin, and IGF-1 expression patterns were upregulated after injury at both the mRNA and protein levels. (bvsalud.org)
  • Pax7, MyoD, and myogenin protein expression levels confirmed the results of the morphometrical analysis. (bvsalud.org)
Differentiation6
  • Activated satellite cells initially proliferate as skeletal myoblasts before undergoing myogenic differentiation. (wikipedia.org)
  • However, Pax7 is expressed prominently after satellite cell differentiation. (wikipedia.org)
  • These dividing cells are known as the "transit amplifying pool" before undergoing myogenic differentiation to form new (post-mitotic) myotubes. (wikipedia.org)
  • One of the first roles described for IGF-1 was its involvement in the proliferation and differentiation of satellite cells. (wikipedia.org)
  • Long non-coding RNAs (lncRNAs) are involved in the process of muscle cell differentiation and play an important role. (bvsalud.org)
  • Using synchronized cultures of C2C12 myoblasts to model these divergent programs, we show that p8 (also known as Nupr1 ), a G1-induced gene, negatively regulates the cell cycle and promotes myogenic differentiation. (biologists.com)
Gene2
Undergo3
  • they neither differentiate nor undergo cell division. (wikipedia.org)
  • When muscle cells undergo injury, quiescent satellite cells are released from beneath the basement membrane. (wikipedia.org)
  • After several cell divisions, the satellite cells begin to fuse with the damaged myotubes and undergo further differentiations and maturation, with peripheral nuclei as in hallmark. (wikipedia.org)
Hepatocyte growth3
Promotes1
  • HGF is produced and secreted by adjacent stromal and mesenchymal cells, it contributes to the development of epithelial organs in a paracrine fashion, exerts regenerative effects on epithelia in the liver, kidney, lung, and other tissues, and promotes the regression of fibrosis in numerous organs ( 7 , 8 ). (spandidos-publications.com)
Protein1
  • To further define which neurons express Eph, antibodies were generated to the cytoplasmic portion of the Eph protein. (sdbonline.org)
Stem cells3
  • Myosatellite cells, also known as satellite cells, muscle stem cells or MuSCs, are small multipotent cells with very little cytoplasm found in mature muscle. (wikipedia.org)
  • This interferes with the maintenance of activated muscle stem cells and impairs muscle growth and repair. (bvsalud.org)
  • Jyotsna Dhawan ABSTRACT Adult stem cells persist in mammalian tissues by entering a state of reversible quiescence, referred to as G 0 , which is associated with low levels of transcription. (biologists.com)
Adult2
  • These cells represent the oldest known adult stem cell niche, and are involved in the normal growth of muscle, as well as regeneration following injury or disease. (wikipedia.org)
  • Adult regenerative myogenesis is dependent on progenitor cells. (biologists.com)
Nuclear1
Immunohistochemistry1
  • Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. (nature.com)
Regeneration1
  • Conditional Met KO mice were generated using Cre‑loxP methodology and characterization of these mice indicated that the HGF‑Met signaling pathway is essential in regeneration, protection, and homeostasis in various tissue types and cells. (spandidos-publications.com)
Genes2
  • Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
  • Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
Differentiate3
  • More specifically, upon activation, satellite cells can re-enter the cell cycle to proliferate and differentiate into myoblasts. (wikipedia.org)
  • Satellite cells are able to differentiate and fuse to augment existing muscle fibers and to form new fibers. (wikipedia.org)
  • Activated satellite cells also begin expressing muscle-specific filament proteins such as desmin as they differentiate. (wikipedia.org)
Development1
Cytoplasmic1
  • On the other hand, activated satellite cells have an increased number of caveolae, cytoplasmic organelles, and decreased levels of heterochromatin. (wikipedia.org)
Cycle2
Define1
  • CD34 and Myf5 markers specifically define the majority of quiescent satellite cells. (wikipedia.org)
Studies1
  • Studies rely almost exclusively on Flow cytometry and fluorescence activated cell sorting (FACS) analysis, which gives no information about cell lineage or behaviour. (wikipedia.org)
Results1
  • for example, greater activation results in the progressive loss of Pax7 expression as they enter the proliferative stage. (wikipedia.org)
Multiple1
  • As such, the satellite cell niche is relatively ill-defined and it is likely that it consists of multiple sub-populations. (wikipedia.org)
Undergoing myogenic differentiation2
  • Activated satellite cells initially proliferate as skeletal myoblasts before undergoing myogenic differentiation. (wikipedia.org)
  • These dividing cells are known as the "transit amplifying pool" before undergoing myogenic differentiation to form new (post-mitotic) myotubes. (wikipedia.org)
Myogenesis4
  • Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
  • PAX3 SMAD6 BMP signaling Hox Myogenic progenitor cell Myogenesis Muscle fiber Embryonic muscle Fetal muscle Limb muscle Patterning Mouse Association Française contre les Myopathies http://dx.doi.org/10.13039/100013465 20313 22234 19507 21357 Agence. (silverchair.com)
  • Pax7 Six1 Six4 Homing Stem cells Myogenesis Muscle development in mouse takes place from embryonic day (E) 8. (silverchair.com)
  • Deletion of Myh3 during embryonic myogenesis leads to the depletion of the myogenic progenitor cell pool and an increase in the myoblast pool, whereas fetal myogenesis -specific deletion of Myh3 causes the depletion of both myogenic progenitor. (silverchair.com)
Proliferation6
  • Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. (nature.com)
  • Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
  • Overexpression of inhibitory human SMAD6 (huSMAD6) in limb MPCs abrogated BMP signaling, impaired their migration and proliferation, and accelerated myogenic lineage progression. (bvsalud.org)
  • Optimum dose of TRF, morphological observation, activity of senescence-associated β -galactosidase (SA- β -galactosidase), and cell proliferation were determined. (hindawi.com)
  • 50 μ g/mL TRF treatment exhibited the highest cell proliferation capacity. (hindawi.com)
  • The activity of SA- β -galactosidase was significantly reduced and cell proliferation was significantly increased in the posttreatment group whereas there was no significant difference in SA- β -galactosidase activity and proliferation capacity of pretreatment group as compared to SIPS-induced myoblasts. (hindawi.com)
Lineage1
  • Studies rely almost exclusively on Flow cytometry and fluorescence activated cell sorting (FACS) analysis, which gives no information about cell lineage or behaviour. (wikipedia.org)
PAX32
  • Current thinking is that most satellite cells express PAX7 and PAX3. (wikipedia.org)
  • Satellite cells in the head musculature have a unique developmental program, and are Pax3-negative. (wikipedia.org)
Progenitor1
  • The canonical view holds that naïve limb muscle progenitor cells (MPCs) invade a pre-established pattern of muscle connective tissue, thereby forming individual muscles. (bvsalud.org)
Quiescent satellite cells3
  • CD34 and Myf5 markers specifically define the majority of quiescent satellite cells. (wikipedia.org)
  • When muscle cells undergo injury, quiescent satellite cells are released from beneath the basement membrane. (wikipedia.org)
  • The quiescent satellite cells were activated in response to damage or exercise and proliferated as myoblasts, which further differentiated and fused to repair or form the muscle fibers [ 7 ]. (hindawi.com)
Myoblasts4
  • More specifically, upon activation, satellite cells can re-enter the cell cycle to proliferate and differentiate into myoblasts. (wikipedia.org)
  • SIPS-induced myoblasts exhibit large flattened cells and prominent intermediate filaments (senescent-like morphology). (hindawi.com)
  • Based on the data, we hypothesized that TRF may reverse the myoblasts aging through replenishing the regenerative capacity of the cells. (hindawi.com)
  • These satellite cells were known as myoblasts once they were isolated from muscle biopsies and proliferate in culture [ 6 ]. (hindawi.com)
Myofibers2
  • There is also evidence suggesting that these cells are capable of fusing with existing myofibers to facilitate growth and repair. (wikipedia.org)
  • Satellite cells proliferate following muscle trauma and form new myofibers through a process similar to fetal muscle development. (wikipedia.org)
Limb1
  • Here, we show that early murine embryonic limb MPCs highly accumulate pSMAD1/5/9, demonstrating active signaling of bone morphogenetic proteins (BMP) in these cells. (bvsalud.org)
Proteins1
  • Activated satellite cells also begin expressing muscle-specific filament proteins such as desmin as they differentiate. (wikipedia.org)
Stem4
  • Myosatellite cells, also known as satellite cells, muscle stem cells or MuSCs, are small multipotent cells with very little cytoplasm found in mature muscle. (wikipedia.org)
  • These cells represent the oldest known adult stem cell niche, and are involved in the normal growth of muscle, as well as regeneration following injury or disease. (wikipedia.org)
  • The field of satellite cell biology suffers from the same technical difficulties as other stem cell fields. (wikipedia.org)
  • The genesis of myogenic stem cells and their homing process during development are poorly known processes. (silverchair.com)
Muscle fibers2
  • Myosatellite cells are located between the basement membrane and the sarcolemma of muscle fibers, and can lie in grooves either parallel or transversely to the longitudinal axis of the fibre. (wikipedia.org)
  • Satellite cells are able to differentiate and fuse to augment existing muscle fibers and to form new fibers. (wikipedia.org)
Significantly1
  • We demonstrated that MCs can be loaded into shed microvesicles with greater efficiency than their parental plasmid counterparts and that microvesicle-mediated MC delivery led to significantly higher and more prolonged transgene expression in recipient cells than microvesicles loaded with the parental plasmid. (regenerativemedicine.net)
Human1
  • Moreover, both quiescent and activated human satellite cells can be identified by the membrane-bound neural cell adhesion molecule (N-CAM/CD56/Leu-19), a cell-surface glycoprotein. (wikipedia.org)
Sarcolemma1
  • These quiescent mononucleated cells are sequestered between the basal lamina and sarcolemma of myofibres, as originally described by Mauro [ 5 ]. (hindawi.com)
Activation2
  • for example, greater activation results in the progressive loss of Pax7 expression as they enter the proliferative stage. (wikipedia.org)
  • Activation of the Wnt pathway in JARID2 depleted cells caused β-catenin to translocate to the nucleus, where it bound to and activated the Myod1 promoter. (biomedcentral.com)
Identify1
  • HGF testing is also used to identify active satellite cells. (wikipedia.org)
Leads1
  • This leads to disassembly of the destruction complex releasing free β-catenin in the cytoplasm, which later translocates to the nucleus and activates gene expression in concert with the T cell factor (TCF) and lymphoid enhancer-binding factor (LEF) families of transcription factors. (biomedcentral.com)
Expression1
  • Microvesicles loaded with MCs encoding a thymidine kinase (TK)/nitroreductase (NTR) fusion protein produced prolonged TK-NTR expression in mammary carcinoma cells. (regenerativemedicine.net)
Response1
  • In response to mechanical strain, satellite cells become activated. (wikipedia.org)
Active1
  • In vivo delivery of TK-NTR and administration of prodrugs led to the effective killing of both targeted cells and surrounding tumor cells via TK-NTR-mediated conversion of co-delivered prodrugs into active cytotoxic agents. (regenerativemedicine.net)