Fetal primitive hematopoietic stem cells. Medical search. Frequent questions

Hematopoiesis involves a series of differentiation steps from one progenitor cell to a more committed cell type, forming the recognizable tree seen in the adjacent diagram. (wikipedia.org)
There are 2 types of hematopoiesis that occur in humans: Primitive hematopoiesis - blood stem cells differentiate into only a few specialized blood lineages (typically isolated to early fetal development). (wikipedia.org)
The pioneering work of Till and McCulloch in 1961 experimentally confirmed the development of blood cells from a single precursor hematopoietic stem cell (HSC), creating the framework for the field of hematopoiesis to be studied over the following decades. (wikipedia.org)
Primitive hematopoiesis is first found in the blood islands (Pander's islands) of the yolk sac at E7.5 (embryonic day 7.5) in mice and 30dpc (30 days post-conception) in humans. (wikipedia.org)
The first wave, defined as primitive hematopoiesis, takes place in the extraembryonic yolk sac and generates transitory hematopoietic cell populations consisting of primitive erythrocytes. (amegroups.org)
The second wave, referred to as definitive hematopoiesis, temporarily occurs in the fetal liver and transitions to the bone marrow and thymus, where multipotent hematopoietic stem cells (HSCs) contribute to the generation of all blood lineages ( 1 - 3 ). (amegroups.org)
In zebrafish, primitive hematopoiesis occurs at early somite stages in the intermediate cell mass (ICM) and the rostral blood island (RBI). (amegroups.org)
Despite these apparent differences in definitive hematopoiesis between fish and mammals, the definitive HSC niche functions to actively recruit stem cells and maintain their plasticity throughout adulthood ( 2 , 4 , 5 ). (amegroups.org)
There is growing evidence that during primitive hematopoiesis in the developing embryo that macrophages generated in the yolk sac are involved in the regulation of primitive HSC trafficking-a mechanism suggested to involve in the secretion of inflammatory factors, extracellular matrix (ECM) components, and metalloproteinases ( 3 , 9 , 10 ). (amegroups.org)
Figure 1 VCAM-1 + macrophages guide hematopoietic stem/progenitor cell to sites of definitive hematopoiesis. (amegroups.org)
Primitive hematopoiesis occurs at early somite stages in the ICM and the RBI. (amegroups.org)
Subset of macrophages expressing ICAM-1 migrate to the sites of definitive hematopoiesis in AGM and CHT to recruit hematopoietic stem cells and initiate neonatal hematopoiesis and guide them further to sites of adult and definitive hematopoiesis in kidneys and thymus. (amegroups.org)
Gene targeting experiments have demonstrated that the transcription factor SCL is essential for primitive and definitive hematopoiesis in the mouse. (ozgene.com)
Acute myeloid leukemia (AML) is a caricature of normal hematopoiesis driven from leukemia stem cells (LSC) that share some hematopoietic stem cell (HSC) programs including responsiveness to inflammatory signaling. (jdstemcellresearch.ca)
Mouse hematopoiesis was thought to develop initially in the yolk sac, where primitive hematopoietic development occurs [ 4 ]. (biomedcentral.com)
This development is also often referred to as fetal hematopoiesis, and was demonstrated by the production of embryonic erythrocytes that still possess a nucleus, express fetal hemoglobin, and lack any lymphoid potential [ 5 ]. (biomedcentral.com)
Their results indicated that yolk sac progenitors before the onset of circulation did not have LT reconstitution potential while progenitors isolated from the AGM did, which indicates that primitive and definitive hematopoiesis may occur at distinct anatomical locations [ 10 ]. (biomedcentral.com)
Our data suggest that zebrafish Runx1 functions in both blood and vessel development at the hemangioblast level, and contributes to both primitive and definitive hematopoiesis. (biologists.com)
Because it is definitely impossible to analyze hematopoietic functions in human being fetuses much of the knowledge on embryonic hematopoiesis comes from studies on mouse embryos [2]. (cancerhugs.com)
Similar to tissue-resident macrophages, mast cells are of dual ontogeny and are generated during both fetal and adult ("definitive") hematopoiesis. (digs-bb.de)
We portrayed the Notch system in embryonic stem cell (ESC)-derived embryoid bodies (EBs) differentiating under the standard protocols used to assess yolk sac (YS) hematopoiesis in vitro. (karger.com)
The stroma is not directly involved in the primary function of hematopoiesis, but it provides the microenvironment and colony-stimulating factors needed to facilitate hematopoiesis by the parenchymal cells. (medscape.com)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. (thermofisher.com)
Hematopoietic stem and progenitor cells (HSPCs) that are essential for establishment and maintenance of the adult blood system are generated during embryonic definitive hematopoiesis. (hirschilabuva.com)

Many human blood cells, such as red blood cells (RBCs), immune cells, and even platelets all originate from the same progenitor cell, the hematopoietic stem cell (HSC). (wikipedia.org)
The CMP can then further differentiate into the megakaryocyte-erythroid progenitor cell (MEP), which goes on to make RBCs and platelets, or the granulocyte/macrophage progenitor (GMP), which gives rise to the granulocytes of the innate immune response. (wikipedia.org)
The hemangioblast theory, which posits that the RBCs and ECs derive from a common progenitor cell, was developed as researchers observed that receptor knockout mice, such as Flk1-/-, exhibited defective RBC formation and vessel growth. (wikipedia.org)
As few transcriptional changes underlie this transition, we used single-cell and bulk assay for transposase-accessible chromatin sequencing (ATAC-seq) on human HSCs and hematopoietic stem and progenitor cell (HSPC) subsets to uncover chromatin accessibility signatures, one including LT-HSCs (LT/HSPC signature) and another excluding LT-HSCs (activated HSPC [Act/HSPC] signature). (jdstemcellresearch.ca)
The present invention relates generally to improved methods and pharmaceutical compositions for mobilizing hematopoietic stem and progenitor cell from bone marrow into peripheral blood by administration of at least one inhibitor of a GTPase, such as Rac1 and/or Rac2 GTPase. (justia.com)
The various mature blood cell types are all ultimately derived from a single class of progenitor cell known as hematopoietic stem cells. (justia.com)
Progenitor cell (stem cell) lines in the bone marrow produce new blood cells and stromal cells. (medscape.com)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)

This event requires a special environment, termed the hematopoietic stem cell niche, which provides the protection and signals necessary to carry out the differentiation of cells from HSC progenitors. (wikipedia.org)
We have used RT-PCR to screen pluripotent murine embryonic stem cells to identify receptor tyrosine kinases (RTKs) potentially involved in the determination or differentiation of cell lineages during early mouse development. (silverchair.com)
Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
Differentiation of HSCs first occurs in the fetal liver, giving rise to mature blood cells. (hal.science)
HSCs and progenitor cells exit the fetal liver and colonize the spleen, where differentiation to the myeloid lineage and particular lymphoid subsets is favored. (hal.science)
a) The cells should exhibits plastic adherence (b) The cell should possess specific set of cell surface markers, i.e. cluster of differentiation (CD) 73, D90, CD105 and lack expression of CD14, CD34, CD45 and human leucocyte antigen-DR (HLA-DR). (c) The cells should have the ability to differentiate in vitro into adipocyte, chondrocyte and osteoblast. (stemcellsgroup.com)
Sphingosine-1-Phosphate Receptor 3 Potentiates Inflammatory Programs in Normal and Leukemia Stem Cells to Promote Differentiation. (jdstemcellresearch.ca)
These signatures inversely correlated during early hematopoietic commitment and differentiation. (jdstemcellresearch.ca)
Differentiation of human pluripotent stem cells (hPSCs) into functional cell types is a crucial step in cell therapy. (ashpublications.org)
The production of terminally differentiated cells from such cells, therefore, requires successive changes of cell fates, meaning that genes relevant for the next stage of differentiation must be switched on and genes not relevant anymore must be switched off. (amegroups.org)
The development of the hematopoietic system has long served as model to understand how changes in gene regulatory networks drive the differentiation of the various blood cell types. (amegroups.org)
To investigate the proliferation and differentiation of hematopoietic stem and progenitor cells in their physiologic environment, we use state-of-the-art mouse models. (digs-bb.de)
We hypothesize that the continuous but rare differentiation of quiescent HSCs into rapidly dividing progenitors limits mutation accumulation in the hematopoietic system. (digs-bb.de)
Sequential rounds of division and differentiation give rise to an enormous amplification of cell numbers, necessary for the production of mature blood cells. (justia.com)
This process of division and differentiation is subject to regulation at many levels to control cell production. (justia.com)
Objective: To establish cultures of cells from the pulp of permanent teeth by the explant method assessing parameters usually presented by stem cells, such as the expression of certain markers and the differentiation ability into osteogenic, adipogenic, and chondrogenic lineages. (bvsalud.org)
In addition, cells were assessed for multi-differentiation potential. (bvsalud.org)
Similarly, the cells showed differentiation ability into all three lineages of cells tested. (bvsalud.org)
The isolated cells may be considered stem cells, based on the current criteria for their characterization, such as plastic adherence, expression of certain markers, and the absence of others, as well as multi-differentiation potential, which showed to be promising for the application in tissue regeneration. (bvsalud.org)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Overexpression of FLI1 in erythroblasts causes inhibition of differentiation and ultimately the development of pre-T cell lymphoblastic leukemia/lymphoma [ 11 ]. (biomedcentral.com)
The Hirschi Lab is broadly interested in endothelial cell differentiation, proliferation, specification and function during development. (hirschilabuva.com)
The lab also uses human stem cell culture models to study the regulation of human endothelial cell differentiation and specification, and to study the functional roles of endothelial cells in regulating tissue-resident stem cells. (hirschilabuva.com)
Regulation of endothelial cell differentiation and specification. (hirschilabuva.com)
EPO supports the proliferation and differentiation of erythroid progenitor cells and is critical for their survival [ 1 ]. (biomedcentral.com)

Dexter observed that mesenchymal stromal cells could maintain early HSCs ex vivo, and both Lord and Gong showed that these cells localized to the endosteal margins in long bones. (wikipedia.org)
However, in the present study we have employed a sensitive reporter-based approach to demonstrate that Pf4-Cre also recombines in a significant proportion of both fetal liver and bone marrow hematopoietic stem cells (HSCs), including the most primitive fraction containing the long-term repopulating HSCs. (nih.gov)
Finally, we show for the first time that Pf4 transcripts are present in adult HSCs and primitive hematopoietic progenitor cells. (nih.gov)
These HSCs are primarily derived from endothelial cells within the ventral wall of the dorsal aorta. (amegroups.org)
The definitive HSCs in mammals first migrate to and repopulate in the fetal liver and then home to the bone marrow, while the definitive HSCs in zebrafish migrate first to the caudal hematopoietic tissue and then to their definitive anatomical sites in the kidney and thymus ( 5 - 8 ). (amegroups.org)
Classical markers for hematopoietic stem cells (HSCs), such as CD90 and CD49F, were largely preserved, whereas CD135 (FLT3) and CD123 (IL3R) had a ubiquitous expression pattern capturing heterogenous populations. (lu.se)
Blood cells are continually produced from a pool of progenitors that derive from hematopoietic stem cells (HSCs). (hal.science)
They undergo maturation into adult HSCs in the aorta or in the fetal liver accompanied by the expression of MHC class I, CD45, CD150, Sca-1 and the absence of CD48. (hal.science)
HSCs also expand in the fetal liver, and in a short time period (four days in the mouse embryo), they increase over 40-fold. (hal.science)
Our long-term objectives are to understand the genetic pathways that control human hematopoietic stem cells (HSCs) and to determine how changes in these programs lead to generating leukemic stem cells (LSC). (jdstemcellresearch.ca)
Although inflammation dysregulates mature myeloid cells and influences stemness programs and lineage determination in HSCs by activating stress myelopoiesis, such roles in LSCs are poorly understood. (jdstemcellresearch.ca)
Here we identified distinct subsets of human long-term (LT)-HSCs that responded differently to regeneration-mediated stress: an immune checkpoint ligand CD112 lo subset that exhibited a transient engraftment restraint (termed latency) before contributing to hematopoietic reconstitution and a primed CD112 hi subset that responded rapidly. (jdstemcellresearch.ca)
Lifelong blood production requires long-term hematopoietic stem cells (LT-HSCs), marked by stemness states involving quiescence and self-renewal, to transition into activated short-term HSCs (ST-HSCs) with reduced stemness. (jdstemcellresearch.ca)
These more advanced hematopoietic progenitors isolated from the aorta-gonad-mesonephros (AGM) region at embryonic day 9.5 to 10 can give rise to lymphoid lineages and are LT reconstituting definitive HSCs, but since this time point is after the establishment of the circulatory system in the embryo their source was difficult to determine [ 13 ]. (biomedcentral.com)
Hematopoietic stem cells (HSCs) are at the top of the hematopoietic hierarchy and can differentiate into all types of mature blood cells throughout life. (digs-bb.de)
According to this concept, HSCs are activated by an increased demand for mature blood cells in situations such as systemic infection or severe blood loss. (digs-bb.de)

As the embryo requires rapid oxygenation due to its high mitotic activity, these islands are the main source of red blood cell (RBC) production via fusing endothelial cells (ECs) with the developing embryonic circulation. (wikipedia.org)
These cells are generated once in the lifetime from mesoderm derivatives closely related to endothelial cells, during a short period of embryonic development. (hal.science)
Peripherally administered EPO crosses the blood-brain barrier and activates in the brain anti-apoptotic, anti-oxidant and anti-inflammatory signaling in neurons, glial and cerebrovascular endothelial cells and stimulates angiogenesis and neurogenesis. (biomedcentral.com)

Preconditioning of recipients by lethal irradiation or chemotherapy forces transplanted hematopoietic stem and progenitor cells to realize their maximum potential, but this does not necessarily reflect their fate and behavior in the native bone marrow environment. (digs-bb.de)

Using CITE-seq (cellular indexing of transcriptomes and epitopes by sequencing), the molecular profile of established immunophenotype-gated progenitors was analyzed in the fetal liver (FL). (lu.se)
Culture of sorted bone marrow populations on stromal cells secreting interleukin-7 or in fetal thymic organ cultures showed that B and T lymphoid progenitors were also present in the lacZhigh and lacZint fractions. (ozgene.com)
The second wave occurs in the dorsal aorta and produces multipotential hematopoietic progenitors. (hal.science)
In this assay hematopoietic cells are cultured in MyeloCult™ medium with supportive human or mouse stromal cell layers and tested for their ability to generate myeloid clonogenic progenitors for several weeks as assayed by replating the cells in a colony-forming unit (CFU) assay using MethoCult™ medium. (stemcell.com)
Establish stromal cell layers and culture hematopoietic progenitors in "Dexter-type" long-term bone marrow cultures. (stemcell.com)
In adult animals, macrophages are derived from circulating monocytes originating in the bone marrow, but much of the tissue-resident population arise from erythro-myeloid progenitors (EMPs) in the extra-embryonic yolk sac, appearing around the same time as primitive erythroblasts. (edu.au)
Early in murine embryonic development, hematopoietic progenitors can be found in the para-aortic splanchnopleura region on embryonic day 7 (as depicted in Figure 1 A), but these progenitors are not LT reconstituting although they do possess lymphoid potential [ 11 - 13 ]. (biomedcentral.com)
Cumano and colleagues' approach was to culture explants containing hematopoietic progenitors isolated from the yolk sac and the embryo proper before the onset of circulation, and to determine their LT reconstitution potential [ 10 ]. (biomedcentral.com)
Taken together, these fundamental studies provided the basis that the synovial macrophages were derived from hematopoietic progenitors and represented a heterogeneous population in both normal and inflamed synovium. (musculoskeletalkey.com)

The first wave occurs in the yolk sac, in mammals or equivalent embryonic structure, and produces nucleated primitive erythrocytes that provide the embryo with the first oxygen transporter and are, therefore, essential for the viability of the embryo. (hal.science)
The yolk sac also produces myeloid cells that migrate to the central nervous system and to the skin to form the microglia and skin specific macrophages, the Langerhans cells. (hal.science)
In mammals the yolk sac acts as a source of primordial germ cells and blood cells. (edu.au)
Note that in early human development (week 2) a transient structure called the "primitive yolk sac" forms from the hypoblast layer, this is an entirely different structure. (edu.au)
In conclusion, we have established a protocol to isolate and propagate EMs in vitro, have further defined specialized properties of yolk-sac-derived macrophages, and have identified EM-EC and EM-NSPC interactions as key inducers of EC tube formation and microglial cell maturation, respectively. (edu.au)
During the development of the embryonic hematopoietic system placenta yolk sac and the pre-fusion allantois are recognized as the hematopoietic organs in mammals [18]-[21]. (cancerhugs.com)
It has been found that the murine embryonic yolk sacs contain the most primitive hematopoietic pluripotent stem cells-the yolk sac hematopoietic stem cells (YS-HSC) [22]. (cancerhugs.com)

The first source of Mesenchymal stem cells was in the bone marrow and considered to be the gold standard for clinical research, although various other sources have being discovered which include: Adipose tissue, Dental pulp, Mobilised Peripheral blood, Amniotic fluid, Joint synovium, synovial fluid, Endosteum, Periosteum, Menstrual blood and birth-derived tissues. (stemcellsgroup.com)
Mesenchymal stem/stromal cells (MSCs) can be isolated from neonatal tissues, most of which are discarded after birth, including placental tissues, fetal membranes, umbilical cord, and amniotic fluid. (stemcellsgroup.com)
Thus, neonatal tissues are easily available and they have biological advantages in comparison to adult sources that make them a useful source for stem cells including MSCs. (stemcellsgroup.com)
We assessed whether cell cycle inhibitors that restrict stem cell populations in other tissues may participate in limiting neural stem cell reactivity in vivo. (rupress.org)
Programs also support the development of novel cell-based therapies to bring the expertise of transfusion medicine and stem cell technology to the repair and regeneration of human tissues and organs. (hhs.gov)
Cells referred to as MSCs originating from various tissues are now used in clinical trials. (biomedcentral.com)
The subject's blood can be processed and used to repopulate the destroyed lymphohematopoietic system of a recipient and may in the future be utilized to repair a variety of non-hematopoietic tissues. (justia.com)
Over the last years, dentistry has been exploring the potential application of stem cells from different origins in the regeneration of oral tissues that were lost or damaged by disease or trauma 1 . (bvsalud.org)
Cells Tissues Organs (2011) 193 (4): 239-252. (karger.com)
When the oxygen content of body tissues is low, if there is loss of blood or anemia, or if the number of red blood cells decreases, the kidneys produce and release erythropoietin, a hormone that stimulates the bone marrow to produce more red blood cells. (medscape.com)
Results: In this study, we found high expression of the HOXD9 gene transcript in glioma cell lines and human glioma tissues by quantitative real-time PCR. (123dok.net)
Vascular perfusion is required for all tissues in the body to provide nutrients and oxygen, remove waste products, maintain fluid homeostasis, and traffic immune cells. (hirschilabuva.com)
Unlike some other tissues, it has not been possible to identify or purify neural stem cells directly from the tissue. (elifesciences.org)
Micha's interest lies in discovering mechanisms that regulate the formation of the embryonic tissues using iPS cells, in order to create novel models for human disease and drug development with emphasis on neurodegeneration. (universiteitleiden.nl)

Mesenchymal stem cells ( MSCs) are known to be multipotent stromal cells that can differentiate into a variety of cell types which include osteoblasts (bone cells), chondrocytes (cartilage cells), myocytes (muscle cells) and adipocytes (fat cells which give rise to marrow adipose tissue). (stemcellsgroup.com)
MyeloCult™ media have been developed for the long-term culture of hematopoietic progenitor cells in association with adherent stromal cells. (stemcell.com)
Because of their multi/pluripotency and immunosuppressive properties, mesenchymal stem/stromal cells (MSCs) are important tools for treating immune disorders and for tissue repair. (biomedcentral.com)
One type of stem cell is involved in producing blood cells and the other is involved in producing stromal cells, which are responsible for the supporting stroma. (medscape.com)
Background: Stromal cells are a functionally important component of human carcinomas. (immune-source.com)
Western blot analysis showed that stromal cells of lung tumours were characterised by a statistically considerably lower phrase level of the p16 proteins as likened with that in regular lung stromal cells. (immune-source.com)

Our data also suggested that expression of SCL was transient and confined to hematopoietic stem and/or progenitor cells, because the differentiated progeny of most lineages (except the erythroid) were beta-galactosidase-negative. (ozgene.com)
Similarly, they are characterized by an extensive capacity for self-renewal, proliferation, potential to differentiate into multiple lineages and their immune-modulatory role on various cells. (stemcellsgroup.com)
The future direction of the study of hematopoietic stem cells will probably make use of pluripotent stem cells to yield specific immune cell lineages and eventual clinical applications. (biomedcentral.com)
This experimental paradigm revealed the existence of a rare population of somatic stem cells that can stably reconstitute all blood lineages lifelong. (digs-bb.de)

In the present study, we demonstrate that functional CD34 + progenitor cells can be efficiently produced from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) by combined modulation of 2 signaling pathways. (ashpublications.org)
Specifically, about the paracrine activities of amniotic fluid stem cells, renal stem cells, embryonic stem cells, mesenchymal stem cell, induced pluripotent stem cells as well as other stem cells. (biomedcentral.com)

Knowledge of human fetal blood development and how it differs from adult blood is highly relevant to our understanding of congenital blood and immune disorders and childhood leukemia, of which the latter can originate in utero. (lu.se)
Direct molecular comparison with an adult bone marrow data set revealed that the HSC state was less frequent in FL, whereas cells with a lymphomyeloid signature were more abundant. (lu.se)
Furthermore, differentially expressed genes between fetal and adult counterparts were specifically analyzed, and a fetal core signature was identified. (lu.se)
Our detailed single-cell map presented herein emphasizes molecular and immunophenotypic differences between fetal and adult blood cells, which are of significance for future studies of pediatric leukemia and blood development in general. (lu.se)
In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
Further studies in mice by Cumano and colleagues sought to address the issue of an anatomical origin of the long-term (LT) reconstituting hematopoietic stem cell (HSC) found in adult animals [ 10 ]. (biomedcentral.com)
Overall, they demonstrated that the definitive HSC differs from the primitive HSC by lymphoid potential as well as in their ability to achieve LT reconstitution following transfer into adult recipients. (biomedcentral.com)
In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. (thermofisher.com)
We prospectively identified, and isolated by flow cytometry, adult mouse lateral ventricle subventricular zone (SVZ) NICs as Glast mid EGFR high PlexinB2 high CD24 −/low O4/PSA-NCAM −/low Ter119/CD45 − (GEPCOT) cells. (elifesciences.org)
Neurons that arise in the adult nervous system originate from neural stem cells and neural progenitor cells. (elifesciences.org)
A region of the brain called the subventricular zone contains both neural stem cells and neural progenitor cells, and is one of only two regions of the brain where neural stem cells are found in adult mammals. (elifesciences.org)
EPO and its receptors (EPOR) are expressed in multiple brain cells during brain development and upregulated in the adult brain after injury. (biomedcentral.com)
The main site of EPO production is fetal liver and adult kidney [ 1 ]. (biomedcentral.com)

Although steady-state conditions revealed no increase in primitive cell proliferation in p21-null mice, a significantly larger fraction of quiescent neural precursors was activated in the hippocampus and subventricular zone after brain ischemia. (rupress.org)
Therefore, p21 is an intrinsic suppressor to neural regeneration after brain injury and may serve as a common molecular regulator restricting proliferation among stem cell pools from distinct tissue types. (rupress.org)
Materials and method: The pulp tissue, obtained from wisdom teeth, was placed in a 6-well plate containing proper culture medium, and stored at 37 °C and 5% CO2 for cell proliferation and plastic adherence. (bvsalud.org)
Conclusion: Our results suggest that the explant method - or cell proliferation method - is suitable for the isolation and culture of stem cells from dental pulp of permanent teeth. (bvsalud.org)
decreased cell proliferation, cell cycle arrest, and induction of apoptosis. (123dok.net)
It was suggested that HOXD9 contributes to both cell proliferation and/or cell survival. (123dok.net)
HOXD9 siRNA treatment of SK-MG-1 SP cells resulted in reduced cell proliferation. (123dok.net)
Conclusions: Our results suggest that HOXD9 may be a novel marker of GCSCs and cell proliferation and/or survival factor in gliomas and glioma cancer stem-like cells, and a potential therapeutic target. (123dok.net)
Homeobox proteins are master regulators of develop-ment and control many cellular processes, including proliferation, apoptosis, cell shape, and cell migration. (123dok.net)
Acute myeloid leukemia (AML) results from the over-proliferation of progenitor cells of the myeloid lineage in the bone marrow. (ghrnet.org)

A year later, Choi showed that blast cells derived from embryonic stem (ES) cells displayed common gene expression of both hematopoietic and endothelial precursors. (wikipedia.org)
In vertebrates, the hematopoietic system develops from two distinct waves or generation of precursors. (hal.science)
Depletion of Runx1 with antisense morpholino oligonucleotides abrogated the development of both blood and vessels, as demonstrated by loss of circulation, incomplete development of vasculature and the accumulation of immature hematopoietic precursors. (biologists.com)
The administration of nonlethal irradiation to induce death of hematopoietic cells and their precursors followed by administration of donor bone marrow (radiation chimeras) revealed populations of macrophages, including synovial macrophages, Langerhans cells, and microglia, that were resistant to irradiation and remained host origin, whereas the monocyte population was derived from donor hematopoietic cells. (musculoskeletalkey.com)

The CLP then goes on to differentiate into more committed lymphoid precursor cells. (wikipedia.org)
and the RBI cells are derived from the lateral mesoderm of the zebrafish head and differentiate into macrophages ( 4 - 8 ). (amegroups.org)
Arnold Caplan (1991), coined the term "mesenchymal stem cell and stated that the cells as multipotent mesenchymal cell populations which can differentiate into several tissue types, and demonstrated roles for MSCs in the regeneration of bone, cartilage or ligaments in animal and clinical studies. (stemcellsgroup.com)
Human embryonic stem cells (hESCs) derived from an early embryo can self-renew indefinitely and differentiate into a variety of cell types. (ashpublications.org)
They could differentiate into bone, cartilage, and a hematopoietic microenvironment. (biomedcentral.com)
As needed, the stem cells differentiate to become a particular kind of cell-a white blood cell, red blood cell, or platelet. (medscape.com)
T lymphocytes originate via lymphoid stem cells that migrate to the thymus and differentiate under the influence of the thymic hormones thymopoietin and thymosin. (medscape.com)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
Neural stem cells can also give rise to neural progenitor cells, which proliferate rapidly during their short lives and then 'differentiate' into neurons or glia. (elifesciences.org)
Since neurosphere-forming cells can self-renew and differentiate into neurons and glia, the ability of cells to form neurospheres has generally been taken as evidence that they are stem cells. (elifesciences.org)

An erythromyeloid-primed multipotent progenitor cluster was identified, potentially representing a transient, fetal-specific population. (lu.se)

These studies and others supported the idea that bone cells create the HSC niche, and all the research that elucidated this specialized hematopoietic microenvironment stemmed from these landmark studies. (wikipedia.org)

Electrophysiological properties were tested using short-circuit current experiments in epithelial cells or freshly isolated mouse intestinal tissue. (bvsalud.org)
Thus, these properties of MSCs make these cells potentially ideal candidates for tissue technology. (stemcellsgroup.com)
The placenta is a fetomaternal organ from which either fetal or maternal tissue can be isolated. (stemcellsgroup.com)
The placenta is a fetomaternal organ consisting of both fetal and maternal tissue, and thus MSC of fetal or maternal origin can be, theoretically isolated. (stemcellsgroup.com)
The cyclin-dependent kinase inhibitor, p21 cip1/waf1 (p21), maintains hematopoietic stem cell quiescence, and we evaluated its role in the regenerative response of neural tissue after ischemic injury using the mice deficient in p21. (rupress.org)
At least two populations of tissue macrophages exist: tissue-resident cells that are embryonically derived and a monocyte-derived population. (musculoskeletalkey.com)
The hematopoietic system is the most regenerative mammalian tissue. (digs-bb.de)
Bone marrow consists of stem cells, which are large, "primitive," undifferentiated cells supported by fibrous tissue called stroma. (medscape.com)
There are 2 main types of stem cells and, therefore, the bone marrow consists of 2 types of cellular tissue. (medscape.com)
Bone marrow can be 1 of 2 types, red or yellow, depending on whether it consists of mainly hematopoietic (and therefore, red-colored) tissue or fatty (and therefore a yellow-colored) tissue. (medscape.com)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
The tissue protective functions of the hematopoietic growth factor erythropoietin (EPO) are independent of its action on erythropoiesis. (biomedcentral.com)

Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)

During this first wave, small number of myeloid cells (e.g., primitive monocytes/macrophages and megakaryocytes) are also generated. (amegroups.org)
Macrophages are well characterized as immune cells. (edu.au)
It is now known that type A cells were synovial macrophages, type B were synovial fibroblasts, and type C were an undetermined population. (musculoskeletalkey.com)
Further studies in the 1980s and early 1990s refined the classification of synovial macrophages with the use of immunohistochemistry and known antibodies to antigen-presenting cells. (musculoskeletalkey.com)
Initial studies suggested that, in adults, a common monocyte/dendritic cell (MDP) progenitor in the bone marrow leads to the development of monocytes, macrophages, and dendritic cells. (musculoskeletalkey.com)
Moreover, parabiotic mice that share the same circulation showed that only a subset of macrophages such as heart, gut, and dermis exhibited a mixed population, whereas Langerhans cells, microglia, and alveolar macrophages were exclusively derived from the parent mouse. (musculoskeletalkey.com)
Although CD4 T cells are the main targets of HIV infection, macrophages also become infected and resist the cytopathic effects of infection, contributing potentially to HIV reservoir persistence. (nih.gov)

In 1978, after observing that the prototypical colony-forming stem cells were less capable at replacing differentiated cells than bone marrow cells injected into irradiated animals, Schofield proposed that a specialized environment in the bone marrow allows these precursor cells to maintain their cellular reconstitution potential. (wikipedia.org)
Bone marrow cells from heterozygous SCLlacZ/w mice were sorted into fractions expressing high, intermediate and low levels of beta-galactosidase (designated lacZhigh, lacZint, and lacZneg). (ozgene.com)
Two-color flow cytometric analysis of TER-119/Erythroid Cells expressed on mouse bone marrow cells. (bdbiosciences.com)
Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) antibody (Cat. (bdbiosciences.com)
Two-color flow cytometric dot plots showing the expressed levels of CD45 versus TER-119/Erythroid Cells (or Ig Isotype control staining) were derived from gated events with the light scattering characteristics of viable bone marrow cells. (bdbiosciences.com)
A higher proportion of CD34 + cells (∼ 20%) could be derived from hPSCs by inhibition of mitogen-activated protein kinase (MAPK) extracellular signal-regulated protein kinase (MEK)/extracellular signal-regulated kinase (ERK) signaling and activation of bone morphogenic protein-4 (BMP4) signaling. (ashpublications.org)
MSCs with a mesodermic origin were first described by Alexander Friedenstein and colleagues in the 1960s and 1970s[ 1 ] as non-hematopoietic bone marrow (BM) cells that adhered to plastic and that could develop into colonies with a fibroblastic appearance. (biomedcentral.com)
Improved methods and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as Rac1 and Rac2 alone or in combination. (justia.com)
Therefore, hematopoietic stem cells mobilized into a subject's peripheral blood by the method of the invention is useful as a source of donor cells in bone marrow transplantation for the treatment of a variety of disorders, including cancer, anemia, autoimmunity and immunodeficiency. (justia.com)
Stem cells represent a very small percentage of bone marrow cells, and are normally quiescent. (justia.com)
These therapies decrease a patient's white blood cell counts, suppress bone marrow hematopoietic activity, and increase the patient's risk of infection and/or hemorrhage. (justia.com)
Current treatments to manage the problems that result from prolonged bone marrow suppression include the reinfusion of a patient's own previously harvested hematopoietic stem and progenitor cells. (justia.com)
In such procedures, patients undergo successive treatments with cell mobilization agents to cause mobilization of hematopoietic progenitor cells from the bone marrow to the peripheral circulation for harvesting. (justia.com)
After harvesting, the patient is given high dose chemotherapy or radiotherapy and the bone marrow function is reconstituted by infusion of the cells harvested earlier. (justia.com)
The use of high-dosage chemotherapy or radiotherapy for bone marrow ablation requires subsequent incorporation of hematopoietic stem cells into the patient, in which case prior harvesting of such cells is required. (justia.com)
The success of treatment crucially depends on the mobilization of the bone marrow stem cells, the subsequent return of which permits the patient to achieve reconstitution of a functioning hematopoietic system. (justia.com)
Bone marrow first appears in the clavicle near the end of fetal life and becomes active about 3 weeks later. (medscape.com)
Bone marrow supersedes the liver as the major hematopoietic organ at 32-36 weeks' gestation. (medscape.com)
Bone marrow thus contains blood cells at varying stages of development. (medscape.com)
Illustration of the pelvis to show the site of bone marrow and blood cells derived from bone marrow. (medscape.com)
Similarly, the bone marrow produces and releases more white blood cells in response to infections, and it produces and releases more platelets in response to bleeding. (medscape.com)
As age progresses, more of the red bone marrow turns into yellow bone marrow and the production of new blood cells becomes more difficult. (medscape.com)
The bone marrow stroma contains mesenchymal stem cells. (medscape.com)
The blood vessels constitute a barrier, inhibiting immature blood cells from leaving the bone marrow. (medscape.com)
Applications Tested: The TEK4 antibody has been tested by blocking of staining with fluorochrome conjugated TEK4 on mouse bone marrow cells. (thermofisher.com)

The self-renewal and proliferative nature of stem cells raised the hope to fight against various diseases. (biomedcentral.com)
True stem cells are both pluripotent-that is they can give rise to all cell types-and capable of self-renewal. (justia.com)
To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)
The lab focuses on multifaceted regulation by transcription and epigenetic factors, lncRNAs, membraneless organelles, mechanisms of self-renewal, and extensively uses organoid technology, single cell RNA sequencing, flow cytometry, and CRISPR-Cas9 genetic engineering approaches. (universiteitleiden.nl)

hPSC-derived CD34 + progenitor cells further developed to endothelial and smooth muscle cells with functionality. (ashpublications.org)
Our results suggest that combined modulation of signaling pathways may be an efficient means of differentiating hPSCs into functional CD34 + progenitor cells. (ashpublications.org)
Leukaemia stem cells (LSC) are identified by high CD34 and negative CD38 expression. (ghrnet.org)

Cells were tested for the expression of surface markers and for ALDH1 enzyme activity, by flow cytometry. (bvsalud.org)
Results: The isolated cells showed high expression of CD44 (98.8%), CD73 (100%), and CD90 (97.2%), and moderate expression of STRO-1 (18.4%) and ALDH1 (16.2%), by flow cytometry. (bvsalud.org)
have used a technique called flow cytometry to identify and isolate neural stem cells and neurosphere-forming cells directly from the subventricular zone. (elifesciences.org)

These data provide a functional correlation between SCL expression and colony-forming ability in immature hematopoietic cells. (ozgene.com)

Mesenchymal stem cells have the ability to expand in many folds in culture while retaining their growth and multilineage potential. (stemcellsgroup.com)

We discuss these properties with examples both from the hematopoietic and embryonic stem cell (ESC) systems. (lu.se)

In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)
Recent studies, however, suggest that MDPs may also develop into other types of hematopoietic cells, such as lymphocytes. (musculoskeletalkey.com)

The common stem cell produces 2 other stem cells, the myeloid stem cell and the lymphoid stem cell. (medscape.com)
Chronic lymphoid leukaemia and hairy cell leukaemia due to chronic exposure to benzene: Report of three cases. (cdc.gov)

However, Ueno and Weissman provided the earliest contradiction to the hemangioblast theory when they saw that distinct ES cells mixed into a blastocyst resulted in more than 1 ES cell contributing to the majority of the blood islands found in the resultant embryo. (wikipedia.org)
In the developing embryo, TFs regulating the assembly/disassembly of transcriptional complexes and ultimately gene expression, are directed by complex extrinsic signalling processes which connect all cells within a multi-cellular organism to their environment. (amegroups.org)

Transient expression of runx1 in cloche embryos resulted in partial rescue of the hematopoietic defect. (biologists.com)

Neural precursor cells from adults have exceptional proliferative and differentiative capability in vitro yet respond minimally to in vivo brain injury due to constraining mechanisms that are poorly defined. (rupress.org)
Un rôle majeur des prostaglandines dans l'hématopoïèse, ainsi mis en évidence, a pu être étendu à la souris chez qui un traitement par la prostaglandine PGE2 provoque la multiplication des CSH in vivo et in vitro, un résultat qui génerera très probablement des applications cliniques. (hypotheses.org)
The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)

Primitive and definitive hematopoietic development. (biomedcentral.com)
Distinct anatomical regions of primitive and definitive hematopoietic development in both the mouse and human. (biomedcentral.com)

A latent subset of human hematopoietic stem cells resists regenerative stress to preserve stemness. (jdstemcellresearch.ca)
(B) Subset of cell surface markers used in the characterization of this developmental process. (biomedcentral.com)

In glioma CSC (GCSC), the expression of some neural stem markers, such as SOX2 and Musa-shi-1, has been reported[6,7]. (123dok.net)
Pre-GEPCOT cells could not form neurospheres but expressed the stem cell markers Slc1a3-CreER T , GFAP-CreER T2 , Sox2 CreERT2 , and Gli1 CreERT2 and were long-lived in vivo. (elifesciences.org)
term pre-GEPCOT cells (based on an acronym of the markers used to isolate the cells), were long-lived and quiescent, but they lacked the ability to form colonies in culture. (elifesciences.org)

Endothelial cell specialization is associated with suppression of cell growth, but how signaling pathways that control endothelial cell cycle and fate are coordinated was not known. (hirschilabuva.com)

The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
What started as a series of microscopic observations in different animal model systems has since evolved into studies of gene expression and regulation, and subsequent protein-protein interactions, cell surface protein expression profiling, and functional mapping of cell fates. (biomedcentral.com)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)

The development of a megakaryocyte lineage specific Cre deleter, using the Pf4 (CXCL4) promoter (Pf4-Cre), was a significant step forward in the specific analysis of platelet and megakaryocyte cell biology. (nih.gov)
These results have fundamental implications for the use of the Pf4-Cre mouse model and for our understanding of a possible role for Pf4 in the development of the hematopoietic lineage. (nih.gov)
Here, a combined single-cell immunophenotypic and transcriptional map of first trimester primitive blood development is presented. (lu.se)
Because Down syndrome leukemogenesis initiates during fetal development, we characterized the cellular and developmental context of preleukemic initiation and leukemic progression using gene editing in human disomic and trisomic fetal hematopoietic cells and xenotransplantation. (jdstemcellresearch.ca)
In this review, we will discuss the milestone discoveries that have been achieved in the field of hematopoietic development, as well as the techniques that have been employed. (biomedcentral.com)
While ER Clark first reported the observation of vascular generation in tadpoles in 1909, and Stockard described blood-vessel development and coined the term angioblast in fish embryos in 1915, it was not until 1920 that F Sabin described the formation of blood cells through the angioblast 'liquefying' within the aorta [ 1 - 3 ]. (biomedcentral.com)
This initial wave of fetal hematopoietic development appears to have short-term reconstituting function, as demonstrated by Dieterlen-Lievre using chicken-quail chimeras [ 6 ]. (biomedcentral.com)
The study established an experimental system for comparison of the hematopoietic toxicity and leukemogenicity of benzene and metabolites during mouse embryonic development and adulthood. (cancerhugs.com)
It is therefore rational to hypothesize that childhood leukemia may have occurred through the fetal stage of development [12]-[16]. (cancerhugs.com)
The hematopoietic stem cells (HSC) are responsible for the development of all blood cells. (cancerhugs.com)
The stimulus for the change of cell fate originates from extrinsic signals which set a cascade of intracellular processes in motion that eventually terminate at the genome leading to changes in gene expression and the development of alternate gene regulatory networks. (amegroups.org)
From development to aging and disease, the brain parenchyma is under the constant threat of debris accumulation, in the form of dead cells and protein aggregates. (frontiersin.org)
Notch receptors and Notch ligands were detected in virtually all cells throughout EB development. (karger.com)
When evaluating with microsatellite steady tumors, there is normally some proof for Kitty least partialC immunological development control in MSI malignancies, like (I) the thick regional lymphocytic infiltration (Crohn's-like lymphocytic response), (II) the elevated apoptotic growth cell amount, and (3) the low amount of isolated metastases that (4) network marketing leads to an improved general individual success [1]C[3]. (immune-source.com)
This remark is certainly a stunning point in favor of a significant Refametinib contribution to growth development control by FSP-specific Testosterone levels cells in vivo , producing those peptides extremely interesting applicants for the advancement of targeted vaccination strategies. (immune-source.com)
Background: HOX genes encode a family of homeodomain-containing transcription factors involved in the determination of cell fate and identity during embryonic development. (123dok.net)
Endothelial Cell Development and Its Application to Regenerative Medicine. (hirschilabuva.com)

The inhibitory effect of LC on CaCCs was primarily on the apical side in epithelial cells, which may be partially produced by affecting cytoplasmic Ca2+ levels. (bvsalud.org)
Although LC activated the cystic fibrosis transmembrane regulator in epithelial cells, its effect was not apparent in colonic epithelium. (bvsalud.org)

runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl , and expression of runx1 is markedly reduced in the zebrafish mutants spadetail and cloche . (biologists.com)

However, all developmental processes originate from pluripotent precursor cell types. (amegroups.org)

This complex process is regulated by the interplay of transcription factors with a chromatin environment, both of which provide the epigenetic information maintaining cell-type specific gene expression patterns. (amegroups.org)
Using a CRISPR Cas9-guided immunoprecipitation assay, we identify a circular RNA in the FLI1 promoter chromatin complex, consisting of FLI1 exons 4-2-3, referred to as FECR1.Overexpression of FECR1 enhances invasiveness of MDA-MB231 breast cancer cells. (biomedcentral.com)

S1PR3-mediated inflammatory signatures varied in a continuum from primitive to mature myeloid states across cohorts of patients with AML, each with distinct phenotypic and clinical properties. (jdstemcellresearch.ca)

The cells were then stained with PE Rat Anti-Mouse CD45 antibody (Cat. (bdbiosciences.com)

Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)

Blood formation occurs in waves that overlap in time and space, adding to heterogeneity, which necessitates single-cell approaches. (lu.se)
Thus, even in stem cells, except for embryonal stem cells and cancer stem cells, telomere shortening occurs during replicative ageing, possibly at a slower rate than that in normal somatic cells. (nature.com)

In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

In clinical practice, she concentrates on germ cell tumors, while her epidemiologic research primarily focuses on colorectal cancer screening and prevention. (dana-farber.org)
and (3) treatment of germ cell tumors in pediatric patients. (dana-farber.org)
In clinical care, Dr. Frazier is the national expert on germ cell tumors in pediatric patients and oversees the care of these patients referred to DFCI. (dana-farber.org)
In addition, she is cochair of two national protocols that opened in 1999 for the treatment of low-risk and high-risk pediatric germ cell tumors and chair of the COG Germ Cell subcommittee in COG Rare Tumors. (dana-farber.org)

Other cell types derived from hematopoietic stem cells include platelets and erythrocytes. (justia.com)
These stem cells divide to eventually give rise to red blood cells, platelets, and most white blood cells in the red marrow. (medscape.com)
White blood cells last anywhere from a few hours to a few days, platelets for about 10 days, and red blood cells for about 120 days. (medscape.com)

The core gene set could separate subgroups of acute lymphoblastic leukemia by age, suggesting that a fetal program may be partially retained in specific subgroups of pediatric leukemia. (lu.se)
In addition to these basic issues concerning leukemia pathogenesis, we are devising new diagnostic procedures for detecting and monitoring leukemia patients based on molecular genetic abnormalities in the malignant cells. (stanford.edu)
We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)
CC-90009, a novel cereblon E3 ligase modulator, targets acute myeloid leukemia blasts and leukemia stem cells. (jdstemcellresearch.ca)
Depletion of GSPT1 by CC-90009 rapidly induces acute myeloid leukemia (AML) apoptosis, reducing leukemia engraftment and leukemia stem cells (LSCs) in large-scale primary patient xenografting of 35 independent AML samples, including those with adverse risk features. (jdstemcellresearch.ca)
years leukemia is definitely a major form of malignancy in children that evolves in the hematopoietic system and is characterized with Pulegone the production of large amounts of immature white blood cells. (cancerhugs.com)
Overall these research claim that prenatal contact with benzene may boost hematopoietic program DNA instability hereditary susceptibility to cancers and contact with leukemogenic elements in early being pregnant and thereby donate to the elevated occurrence of youth leukemia [17]. (cancerhugs.com)

Then, we will discuss several principles that govern macrophage phagocytosis of apoptotic cells (efferocytosis), including the existence of redundant recognition mechanisms ("find-me" and "eat-me") that lead to a tight coupling between apoptosis and phagocytosis. (frontiersin.org)
Because it is such a diverse population of cells, a fundamental question is whether they have a common precursor or whether each macrophage population develops from a different precursor. (frontiersin.org)

Zebrafish runx1 is expressed in hematopoietic and neuronal cells during early embryogenesis. (biologists.com)

Producing cells according to Good Manufacturing Practices (GMPs) is a global challenge for the production of all cells for use in humans, specifically mesenchymal stem/stroma cells (MSCs), one of the most promising tools in cellular therapy and regenerative medicine. (biomedcentral.com)
Prof. Dr. Micha Drukker is a professor of Stem Cell Biology, Models and Regenerative medicine at the Division of Drug Discovery & Safety. (universiteitleiden.nl)

Neurosphere formation is commonly used as a surrogate for neural stem cell (NSC) function but the relationship between neurosphere-initiating cells (NICs) and NSCs remains unclear. (elifesciences.org)

The biological properties and clinical potential of stem cells elicit that are generated must not be unduly sensitive to small fluctu- continued scientific, commercial, and public interest. (lu.se)
Research in the Hirschi Lab aims to uncover the developmental mechanisms of hemogenic endothelial cell specification and endothelial-to-hematopoietic transition, and apply insights gained to the generation of human HSPCs for clinical therapies. (hirschilabuva.com)
Micha's lab collaborates closely within the science faculty on stem cell organoid models for drug research, toxicity, replacement of animal models, and with the Leiden University Medical Center (LUMC) on stem cell immunology and manufacturing (GMP) of iPSCs for clinical purposes. (universiteitleiden.nl)

Cell-to-cell signalling is induced by specific ligands such as growth factors which activate their cognate receptor molecules. (amegroups.org)

however, the level of telomerase activity is low or absent in the majority of stem cells regardless of their proliferative capacity. (nature.com)
Telomerase can add telomeric repeats onto the chromosome ends, and prevents the replication-dependent loss of telomere and cellular senescence in highly proliferative cells of the germline and in the majority of cancers ( Blasco, 2005 ). (nature.com)
As stem cells have elongated proliferative capacity, they should have a mechanism that maintains telomere length through many cell divisions. (nature.com)
When stimulated to divide, they give rise to more committed, differentiated daughter cells with less proliferative potential, called "early progenitor" cells. (justia.com)
The neurosphere-forming cells, named GEPCOT cells, were short-lived and highly proliferative in the brain. (elifesciences.org)

MicroRNAs (miRNAs) regulate both normal stem cells and CSCs, and dysregulation of miRNAs has an important role in tumorigenesis. (biomedcentral.com)

In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)

Anatomy48

Organisms15

Diseases27

Chemicals and Drugs52

Analytical, Diagnostic and Therapeutic Techniques and Equipment49

Psychiatry and Psychology1

Phenomena and Processes43

Disciplines and Occupations3

Anthropology, Education, Sociology and Social Phenomena1

Information Science3

Named Groups3

Health Care5

Hematopoiesis25

Progenitor cell9

Differentiation38

HSCs16

Endothelial cells3

Transplanted hematopoietic stem and progenitor cells1

Progenitors9

Yolk sac7

Tissues14

Stromal cells6

Lineages4

Pluripotent stem2

Adult13

Proliferation10

Precursors4

Differentiate11

Multipotent1

Specialized hematopoietic microenvironment1

Tissue12

Populations2

Macrophages7

Bone25

Self-renewal4

CD343

Flow Cytometry3

Immature hematopoietic1

Mesenchymal stem1

Embryonic stem1

Lymphocytes2

Lymphoid2

Embryo2

Transient1

Vitro3

Definitive2

Subset2

Markers3

Pathways that control1

Regulation4

Development17

Epithelial cells2

Lateral plate me1

Originate1

Chromatin2

Distinct1

CD451

Organ1

Occurs2

Gene expression1

Tumors4

Platelets3

Leukemia7

Macrophage2

Neuronal1

Regenerative medicine2

Neural stem1

Clinical3

Receptor1

Proliferative5

Regulate1

Somatic cells1