Fetal fibroblasts fetal donor egg cell. Medical search. Frequent questions

Unlike previous attempts to clone monkeys, the donated nuclei came from fetal cells, not embryonic cells. (wikipedia.org)

The researchers from Northwest A&F University in China used a modified version of the CRISPR system called CRISPR/Cas9n to insert a new TB resistance gene - NRAMP1 - into the genome of bovine foetal fibroblasts - a cell derived from female dairy cows. (siasat.com)
2018). Development of bovine embryos in vitro in coculture with murine mesenchymal stem clls and embryonic fibroblasts. (sciendo.com)
Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
Materials and Methods Mammalian cell tradition and harvest HeLa, HEK 293T and A549 cells [13, 14] were cultured in Dulbeccos Modified Eagles Medium (pH7.4) with 10% (v/v) Fetal Bovine Serum at 37C. (irjs.info)

The chickenpox, rubella, hepatitis A, shingles and one rabies vaccine all derive from fetal embryo fibroblast cells cultivated in the 1960s -- and Catholic leadership has wrestled with the ethics of using those vaccines. (salon.com)
A fertilized egg is totipotent, meaning that its potential is total, and as the fertilized egg divides all the cells in the embryo remain totipotent until the fertilized egg has reached the 8/16-cell stage. (biomedcentral.com)
In theory, and to some extent in practice, an 8-cell embryo can be divided into eight single cells, and each cell has the potential of generating an individual if implanted in a woman's uterus. (biomedcentral.com)
Here, we consider the metabolism of the early embryo through development, and look at the nutrient milieu within the developing stem cell niche. (conditionmed.org)
2019). Akin to the dynamic nutrient requirements of the developing embryo, discrete in vitro cell states have distinct metabolic profiles (Zhou et al. (conditionmed.org)
The developing embryo, from which pluripotent stem cells originate, undergoes a series of dynamic metabolic transitions synchronized to its molecular development. (conditionmed.org)
Up to the 2-cell embryo, blastomeres remain totipotent (Garner and McLaren, 1974). (conditionmed.org)
In the mouse, a transporting epithelium is established around the 8-16-cell stage through a process known as compaction where cell definition is lost and the outer cells of the embryo form tight junctions, giving rise to the blastocyst. (conditionmed.org)
Cells in the womb near the embryo are loaded with nutrients. (ehd.org)
Here, we hypothesize that supplementation of embryo culture medium with extracellular vesicles from endometrial-derived mesenchymal stem cells (EV-endMSCs) may have a positive impact on the embryo competence of aged oocytes. (bvsalud.org)
This question had been asked by embryologists since 1886 ( Rauber, 1886 ), and Spemann ( Spemann, 1938 ) had demonstrated by an egg ligation experiment that the nuclei of an eight-cell frog embryo are developmentally totipotent. (biologists.com)
However, Briggs and King ( Briggs and King, 1957 ) had also found that the nucleus of an endoderm cell from a neurula embryo could no longer support normal development ( Fig. 2 ). (biologists.com)
Eva Nagy (College or university of Guelph, Canada), was found in our research after propagating in embryonated poultry eggs at embryo time (ED)9C11. (ap26113.com)
It's hard to find a simplified, but not overly simplified, explanation of how pluripotent or totipotent stem cells become germ cells, or any other cells for that matter, but it begins in the embryo. (ussromantics.com)
A cell signalling process in the embryo induces a small, transient proportion of the cell mass, the primitive streak, to become primordial germ cells (PGCs), along with other cells. (ussromantics.com)
This process is called gastrulation, in which the embryo begins to differentiate into distinct cell lineages. (ussromantics.com)

Here, we developed CellAgeClock, a new epigenetic clock that measures subtle ageing changes in primary human cells in vitro . (biorxiv.org)
Our method expands the scope of CpG methylation profiling from measuring human chronological and biological age from human samples in years, to accurately and rapidly detecting anti-ageing potential of drugs using human cells in vitro , providing a novel accelerated discovery platform to test sought after geroprotectors. (biorxiv.org)
Mouse nuclear transfer embryonic stem cells (NT-ESCs) were first established in 2000, and then proved to be able to differentiate either in vivo or in vitro, and give rise to individual tissues through germ line transmission or tetraploid complementation. (benthamscience.com)
We discuss what is known about the distinct metabolic states captured in vitro by the 2-cell-like, naïve, blastocyst-like, formative, and primed states of pluripotency. (conditionmed.org)
It is only through understanding embryonic metabolism and development that we can derive and maintain different in vitro stem cell states for disease modeling and therapies. (conditionmed.org)
The anticancer activity of biosynthesized ZnO NPs and C. citratus ALE was in vitro tested against seven human cancer cell lines (HCCL) (i.e. (springeropen.com)
survival of erythroid burst-forming units and erythroid colony-forming units in canine bone marrow cells exposed in vitro to 1 mev neutron radiation or x rays. (liverpool.ac.uk)

At this juncture, through the present study it was found that, chicken neural retinal cells when grown alone failed to survive and contrarily when either co-cultured with chicken amniotic epithelial cells / cultured in amniotic epithelial cell conditioned medium not only survived but also showed extensive differentiation. (scielo.org.ar)
The established dogma has however been challenged by recent findings suggesting that multipotent stem cells have a broader differentiation potential than previously thought. (biomedcentral.com)
Stem cells are not specialized and the process of their specialization is called differentiation. (benthamscience.com)
To establish protocols for the safe and efficient differentiation of healthy cells for therapies, we must develop a better understanding of the dynamic continuum of metabolic states that span pluripotency and differentiation, and how to influence them. (conditionmed.org)
The formation of the mammalian respiratory unit is initiated during the transition between the pseudoglandular and canalicular/saccular stages and is characterized by coordinated proliferation and differentiation of epithelial and mesenchymal cells. (biomedcentral.com)
2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
Ideally, iPSC-based therapies in the future will rely on the isolation of skin fibroblasts or keratinocytes, their reprogramming into iPSCs, and the correction of the genetic defect followed by differentiation into the desired cell type and transplantation. (biomedcentral.com)

Herein, we describe a scalable serum-free co-culture system for the expansion of UCB-derived CD34+-enriched cells using microcarrier-immobilized human bone marrow-derived mesenchymal stromal cells as feeder cells. (bvsalud.org)
Oddly enough, the serum MCP-1 Metoclopramide HCl level elevated and peaked at six h after c-lipo pretreatment quickly, suggesting which the MCP-1 made by c-lipo-phagocytized Compact disc68+ Kupffer cells Metoclopramide HCl may recruit Compact disc11b+ macrophages in the periphery and bone tissue marrow. (enmd-2076.com)

The cells giving rise to the foetus, are called pluripotent embryonic stem cells, since they can form every cell type in the body. (biomedcentral.com)
The pluripotent stem cells differentiate further into cells commonly called multipotent stem cells. (biomedcentral.com)
A recent study indicates that even adult humans might harbor cells resembling pluripotent embryonic stem cells. (biomedcentral.com)
Kuldip S. Sidhu , " Frontiers in Pluripotent Stem Cells Research and Therapeutic Potentials Bench-to-Bedside ", Bentham Science Publishers (2012). (benthamscience.com)
Induced pluripotent stem cells, another types of pluripotent stem cells derived from any tissue by reprogramming and are the homologous source of stem cells. (benthamscience.com)
At first, the mesenchymal stem cell was thought to be the primary component of this undifferentiated cell type, however it is now evident that within the adipose extracellular matrix are also adipocytic precursors (known as progenitor cells) adherent to adipocytes, and in close approximation to a variety of additional undifferentiated multipotent and pluripotent cells, including pericytes and endothelial cells, all thought to play important roles in mesenchymal-stromal derived tissue regeneration. (journalofprolotherapy.com)
Derivation of autologous induced pluripotent stem cells (iPSCs) through direct reprogramming of easily accessible somatic cells holds the potential to transform the field of regenerative medicine. (biomedcentral.com)
Here, we summarize current reprogramming methodologies with a focus on the production of transgene-free or genetically unmanipulated iPSCs and highlight important technical details that ultimately may influence the biological properties of pluripotent stem cells. (biomedcentral.com)
Although exciting results have been achieved by means of somatic cell nuclear transfer, cell fusion, and culture-induced reprogramming [ 1 ], these procedures are technically demanding and inefficient and therefore unlikely to become a common approach for producing patient-specific pluripotent cells. (biomedcentral.com)
In 2006, a major breakthrough was reported in Japan by Takahashi and Yamanaka, who described the generation of induced pluripotent stem cells (iPSCs) from mouse fibroblasts via over expression of defined transcription factors [ 2 ]. (biomedcentral.com)
Here, we established induced pluripotent stem cells (iPSCs) derived from MF patient samples (MF-iPSCs) harboring JAK2 V617F, CALR type 1, or CALR type 2 mutations. (ashpublications.org)

A few of the cells in the inner cell mass will develop into the foetus, whereas the rest will form the placenta and other supporting tissues needed for foetal development in the uterus. (biomedcentral.com)
Depending on the source, stem cells can be classified into two broad categories i.e. embryonic stem cells that are derived from embryos and non-embryonic stem cells that are derived from adult and fetal tissues. (benthamscience.com)
Recognition of the vast number of undifferentiated cells associated with the stromal vascular fraction has resulted in extensive research demonstrating the heterogeneity of such cells, and their ability to participate in production of all mesodermal-derived tissues. (journalofprolotherapy.com)
Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
In addition, as the genetic identity of the donor egg from which the ESCs are derived most likely will differ from that of potential recipients, patients who receive ESC-derived cells or tissues may face the same complications that result from organ transplantation (for example, immunorejection, graft-versus-host disease, and need for immunosuppression). (biomedcentral.com)

In the context of hematopoietic cell transplantation, hematopoietic stem/progenitor cells (HSPC) from the umbilical cord blood (UCB) present several advantages compared to adult sources including higher proliferative capacity, abundant availability and ease of collection, non-risk and painless harvesting procedure, and lower risk of graft-versus-host disease. (bvsalud.org)

cells that were extracted from fetal tissue, which was donated. (salon.com)
there are reasons to have conversations about embryonic stem cell research and fetal tissue research. (salon.com)
and altering cell and tissue characteristics for biomedical research and manufacturing. (nationalacademies.org)
International bi-monthly journal of cell signaling, tissue protection, and translational research. (conditionmed.org)
The blastocyst comprises the inner cell mass (ICM), which gives rise to the three primary germ layers and consequently the fetus, and the trophectoderm (TE), which gives rise to the extraembryonic and placental tissue. (conditionmed.org)
One explanation for deficient repair is when undifferentiated adult stem repair cells are inadequate in number or cannot be stimulated within the damaged tissue site. (journalofprolotherapy.com)
With improved understanding of tissue healing and regeneration, stem cell Prolotherapy is gaining significant clinical importance and potential. (journalofprolotherapy.com)
Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
At this micro level, the oxygen is released from the red blood cells into the tissue cells that line the capillaries. (drwells.net)
The oxygen given up by the blood cells is replaced with carbon dioxide released by the tissue cells and carried back to the lungs to be exchanged for more oxygen. (drwells.net)
The tissue is composed of collagens, fibroblasts, and many hyaluronic. (practicalmommy.com)

ABBR: EGF A polypeptide that stimulates growth of several different cells, including keratinocytes. (unboundmedicine.com)

To circumvent these obstacles, considerable effort has been invested in attempting to derive ESC-like cells by reprogramming somatic cells to an embryonic state. (biomedcentral.com)
Several experimental strategies have been developed to derive iPSCs from differentiated somatic cells (summarized in Figure 1 ). (biomedcentral.com)

They extracted nuclei from the fibroblasts of an aborted fetal monkey (a crab-eating macaque or Macaca fascicularis) and inserted them into egg cells (ova) that had had their own nuclei removed. (wikipedia.org)
The team used two enzymes to erase the epigenetic memory of the transferred nuclei of being somatic cells. (wikipedia.org)
The scientists also attempted to clone macaques using nuclei from adult donors, which is much more difficult. (wikipedia.org)
Even advanced donor cells from the endoderm of Xenopus tadpoles have nuclei that can sometimes yield normal individuals after nuclear transfer [data taken from Briggs and King ( Briggs and King, 1957 ) for Rana and from Gurdon ( Gurdon, 1962 ) for Xenopus ]. (biologists.com)

1, 2 Recently, Prolotherapists have begun to utilize the potential of autologous adipose (fat)-derived stem/stromal cells (AD-SC) within non-manipulated fat graft scaffolding, combined with high-density PRP concentrates (HD-PRP) to provide a potent biological therapeutic combination. (journalofprolotherapy.com)
Therefore we have chosen to use the term "adipose-derived stem/stromal cells" (AD-SC's), rather than simply "mesenchymal stem cells. (journalofprolotherapy.com)
Bone marrow stromal cells (BMSCs, also known as bone marrow mesenchymal stem cells) are a plastic-adherent heterogeneous cell population that contain inherent skeletal progenitors and a subset of multipotential skeletal stem cells (SSCs). (bvsalud.org)

In somatic cells, the activity of telomerase, a reverse transcriptase that can elongate telomeric repeats, is usually diminished after birth so that the telomere length is gradually shortened with cell divisions, and triggers cellular senescence. (nature.com)
Thus, even in stem cells, except for embryonal stem cells and cancer stem cells, telomere shortening occurs during replicative ageing, possibly at a slower rate than that in normal somatic cells. (nature.com)
Telomeric DNA consists of short guanine-rich repeat sequences in all eukaryotes with linear chromosomes, and its length in human somatic cells is remarkably heterogeneous among individuals ranging from 5 to 20 kb, according to age, organ, and the proliferative history of each cell ( Wright and Shay, 2005 ). (nature.com)
In most human somatic cells except for stem cells and lymphocytes, telomerase activity is diminished after birth so that telomere length shortens with each cell division. (nature.com)

However, donor organs are in absolute shortage, and sadly, most patients die while waiting for a donor organ. (frontiersin.org)
To take human organ generation via BC and transplantation to the next step, we reviewed current emerging organ generation technologies and the associated efficiency of chimera formation in human cells from the standpoint of developmental biology. (frontiersin.org)
Presently, in the United States, another person is added to an organ transplant list every 10 min, 17 people die each day while waiting for donor organs, and approximately 105,800 patients are waitlisted for an organ transplant according to the health resources and services administration (HRSA). (frontiersin.org)
However, though BC is emerging as a potential organ transplant option, challenges regarding organ size scalability, immune system incompatibilities, long-term maintenance, potential evolutionary distance, or unveiled mechanisms between donor and host cells remain. (frontiersin.org)
The placenta is a mateno-fetal organ which begins developing at implantation of the blastocyst and is delivered with the fetus at birth. (edu.au)
No YFP-labeled cells had been recognized in the liver organ of mice treated using the control vector AAV8-CMV-RFP (B). AAV8-CMV-Cre tagged CK19-expressing cholangiocytes and HNF4-expressing hepatocytes (C,D). Yellowish arrowheads: YFP+CK19+ cholangiocytes. (cancercurehere.com)
The immunohistochemical evaluation from the liver organ and the stream cytometry from the liver organ mononuclear cells demonstrated that clodronate liposome (c-lipo) treatment significantly reduced the spindle-shaped F4/80+ or Compact disc68+ cells, as the oval-shaped F4/80+ Compact disc11b+ cells elevated. (enmd-2076.com)
Furthermore, anti-TNF Ab pretreatment (which reduced the FasL appearance of Compact disc11b+ Kupffer cells), anti-FasL Ab mice or pretreatment attenuated the liver organ injury induced by CCl4. (enmd-2076.com)
It really is popular that liver organ HGFB NK cells and NKT cells potently generate IFN- in response to IL-12 and/or LPS [11]C[13]. (enmd-2076.com)
Oddly enough, liver organ B cells (mainly B-2 cells) generate IL-12 and IFN- however, not IgM, in response to LPS (vice versa for spleen B cells) [14]. (enmd-2076.com)
Furthermore, these IL-12-making liver organ B cells, as opposed to spleen B cells, phagocytose bacterias and eliminate them [15], [16]. (enmd-2076.com)
As a result, these liver organ immune system cells, including B cells and their cytokines, mainly become innate immune effectors against tumors and infections simply by their T helper-1 immune response in the liver organ. (enmd-2076.com)

By virtue of expressions of glial and neural surface markers and capability of neurotransmitter metabolism, amniotic epithelial cells are considered as candidate cell type for transplantation strategies to treat neurological disorders. (scielo.org.ar)
These findings suggested the usefulness of them as an alternate source of cells for transplantation approaches to treat neurological disorders (Sakuragawa et al. (scielo.org.ar)
Transplantation of amniotic epithelial cells in various regions of central nervous system such as caudate nucleus (Bankiewicz et al. (scielo.org.ar)
Amniotic epithelial cells transplantation in Parkinson model of rats was found to reverse the condition and prevent death in neurons (Kakishita et al. (scielo.org.ar)
2001). In our previous report, we concluded the usefulness of amniotic epithelial cells transplantation in spinal cord injury repair research. (scielo.org.ar)
We had also outlined various biological and social advantages by which, amniotic epithelial cells transplantation excels its precedent viz. (scielo.org.ar)
Recently, our conclusion of usefulness of amniotic epithelial cells transplantation to treat spinal cord injuries has been confirmed in rodent models of spinal cord injury (Zhi-yuan et al. (scielo.org.ar)
in 1956 and Till and McCulloch in 1961, demonstrating that lethally irradiated mice could be rescued with cells from freshly isolated bone marrow [ 10 ] and that BM cells formed spleen clonies (CFU-S) following transplantation to irradiated recipients [ 30 ], respectively. (biomedcentral.com)
As a brand new graduate student starting in October 1956, my supervisor Michail Fischberg, a lecturer in the department of Zoology at Oxford, suggested that I should try to make somatic cell nuclear transplantation work in the South African frog Xenopus laevis . (biologists.com)

Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
Percent human being beta cell proliferation in islet grafts from vehicle control (n=6 total, 2 per islet donor) and alogliptin-treated (n=6 total) mice. (healthweblognews.info)
Open in a separate window Figure 5 Insulin+BrdU+ beta cells in human being islet grafts from alogliptin-treated mice. (healthweblognews.info)
Notes: Representative micrographs of human being islets from a single donor (Donor 1) engrafted in (A) vehicle control or (B) alogliptin-treated mice are demonstrated. (healthweblognews.info)
tolerance tests were significantly improved by alogliptin treatment for mice transplanted with islets from two of the three human being islet donors. (healthweblognews.info)
The percentage of insulin+BrdU+ cells in human being islet grafts from alogliptin-treated mice was approximately 10-fold more than from vehicle control mice, consistent with a significant increase in human being beta cell proliferation. (healthweblognews.info)
Summary Human being islet-engrafted immunodeficient mice treated with alogliptin display improved human being insulin secretion and beta cell proliferation compared to control mice engrafted with the same donor islets. (healthweblognews.info)
The goal of this study was to make use of STZ-induced diabetic NSG mice transplanted with human being pancreatic islets to determine the ability of alogliptin to enhance human being beta cell function and proliferation. (healthweblognews.info)
the NSG is an immunodeficient mouse that can be engrafted with functional human being cells and cells for in vivo studies.18 Male NSG mice (8C12 weeks old) received a single intraperitoneal injection of 160 mg/kg STZ (Sigma-Aldrich, St Louis, MI, USA) to induce diabetes (blood glucose 300 mg/dL on two consecutive days). (healthweblognews.info)
reporter mice had been injected with AAV8-CMV-Cre at postnatal day time 2 (P2) and cells had been analyzed at P10 and P56, respectively. (cancercurehere.com)
In today's study, we looked into the immunological function of Kupffer cells/macrophages in CCl4-induced hepatitis in mice. (enmd-2076.com)
The populace of Compact disc11b+ Kupffer cells/macrophages significantly elevated 24 hour (h) after CCl4 administration, in c-lipo-pretreated mice especially. (enmd-2076.com)
It really is today generally accepted which the livers of mice and human beings contain types of innate immune system cells [11]C[13]. (enmd-2076.com)
MF model cells and mice induced by MPL W515L, another type of mutation recurrently detected in MF patients, were used to elucidate the therapeutic potential of CAMK2 inhibition. (ashpublications.org)

As the fetus relies on the placenta for not only nutrition, but many other developmentally essential functions, the correct development of the placenta is important to correct embryonic and fetal development. (edu.au)
A 2009 longitudinal Norwegian study suggests an association between large placenta relative to fetal size "disproportionately large placenta relative to birth weight was associated with increased risk of (adult) cardiovascular disease death. (edu.au)

Multipotent stem cells are present in the adult individual as well as the fetus and umbilical cord. (biomedcentral.com)
Abnormalities can range from anatomical associated with degree or site of inplantation, structure (as with twinning), to placental function, placento-maternal effects (pre-eclampsia, fetal erythroblastosis) and finally mechanical abnormalities associated with the placental (umbilical) cord. (edu.au)
Surrounding the umbilical cord is the egg skin, also called sheepskin or amnion. (practicalmommy.com)

Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
The regulation of telomere length and telomerase activity is a complex and dynamic process that is tightly linked to cell cycle regulation in human stem cells. (nature.com)
Here we review the role of telomeres and telomerase in the function and capacity of the human stem cells. (nature.com)
In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
The CellAgeClock outperforms other epigenetic clocks in measuring subtle ageing changes in primary human cells in culture. (biorxiv.org)
Previously, we have reported neurotrophism exhibited by human amniotic epithelial cells when transplanted after spinal cord injury in bonnet monkeys. (scielo.org.ar)
There are different types of stem cells that best can be described in the context of normal human development [ 23 ]. (biomedcentral.com)
The human body is made up of about 220 different kinds of specialized cells such as nerve cells, muscle cells, fat cells and skin cells. (benthamscience.com)
Embryonic stem cells (ESCs) can grow infinitely and give rise to all types of cells in human body, thus of tremendous therapeutic potentials for a variety of diseases, such as Parkinson's disease, spinal cord injury, and diabetes. (benthamscience.com)
However, appreciation of the human microbiome and host cell-microbe interactions must be taken into consideration as we try to determine what interactions are pathologic. (edu.au)
The potential use of embryonic stem cells (ESCs) for cell replacement therapies is limited by ethical concerns and the technical hurdles associated with their isolation from human embryos. (biomedcentral.com)
It was reported that a human being protein comprising five WD40 repeat domains can stabilize ORC binding to chromatin by interacting with ORC and modulating the chromatin association of ORC in human being cells [12], suggesting that WD40 repeat proteins play important roles in human being DNA replication. (irjs.info)
Here we display that hIPI3 also has important functions in the initiation of DNA replication TMC353121 in human being cells. (irjs.info)

Amniotic epithelial cells were believed to secrete an 'Epidermal Growth Factor (EGF) - like' factor and exact identification was not made. (scielo.org.ar)
Fibroblast Growth Factor - 2 (FGF-2) plays a critical role in retinal development especially in chicken neural retinal development. (scielo.org.ar)
A growth factor produced by the cell that stimulates the same cell to grow. (unboundmedicine.com)

Fully reprogrammed NT-ESCs are indistinguishable from ESCs derived from fertilized eggs functionally and substantially. (benthamscience.com)
What is more, by deriving NT-ESCs from patient cells, the problem of immune rejection may be avoided. (benthamscience.com)

It is interesting to note that while factor secreted by amniotic epithelial cells resembles EGF and/or FGF-2 in its biological action, known isoforms of them were not detected. (scielo.org.ar)
Considering the biological closeness between EGF and FGF-2, results indicate the possibility of a novel isoform of these growth factors secreted by amniotic epithelial cells. (scielo.org.ar)
Amniotic epithelial cells were known to express some of neuronal and glial cell markers (Sakuragawa et al. (scielo.org.ar)
Based on the neurotropism exerted by amniotic epithelial cells conditioned medium, a diffusible neurotrophic factor produced by them had been suggested as a possible cause apart from direct cell-to-cell effects (Uchida et al. (scielo.org.ar)
2001) had found amniotic epithelial cells conditioned medium showed neurotrophic effect on rat embryonic day 18 (E18) cortical neurons. (scielo.org.ar)
15 Diabetes in Pregnancy 263 Fetal Surveillance in Diabetes Biophysical profle takes into consideration fetal moveFirst Trimester ment, respiratory, tone, amniotic fuid largest vertical pocket Early ultrasound for confrmation of viability and dating and nonstress test. (ehd.org)

Until recently it was considered a fact that, for example, hematopoietic stem cells did not have the potential to differentiate into cells of other types than the cells constituting the blood system. (biomedcentral.com)

Placental stem cells. (scielo.org.ar)

Approximately five days after fertilization the totipotent cells have differentiated and started to form a hollow sphere of cells called a blastocyst. (biomedcentral.com)

Stem cells are emerging as an important source of material for diseases in regenerative medicine. (benthamscience.com)
The study of biology of stem cells is the hallmark of the recent emerging field of regenerative medicine and medical biotechnology. (benthamscience.com)

Astrocytes, the most abundant glial cell type in the brain, provide metabolic and trophic support to neurons and modulate synaptic activity. (jneurosci.org)

2003). Similarly, when transplanted into ischemic cortical areas, they were found to differentiate into 'neuron-like' cells (Okawa et al. (scielo.org.ar)
We explore the recently described metabolic surge event that occurs as pluripotency is lost and stem cells commit to differentiate. (conditionmed.org)
The primordial germ cells differentiate in the testis, in these seminiferous tubules… But let me first fast forward to the end of the process and describe a complete, mature sperm cell or spermatozoon. (ussromantics.com)

The endothelial cells that line the intima sense changes in the blood and send signals to the middle layer to help the artery adapt to those changes. (drwells.net)

In the developing lung, the specific temporal-spatial interactions between mesenchymal and epithelial cells are carefully orchestrated to permit the gradual establishment of an effective blood-gas barrier (BGB) [ 1 , 2 ]. (biomedcentral.com)

is a British developmental biologist who was the first to use nuclear transfer of differentiated adult cells to generate a mammalian clone, a Finn Dorset sheep named Dolly, born in 1996. (mathisfunforum.com)

She added that donors "can stipulate where their money goes and can ask that it not pay for embryonic stem cell research. (salon.com)

Wikinews has related news: Healthy cloned monkeys born in Shanghai Since scientists produced the first cloned mammal Dolly the sheep in 1996 using the somatic cell nuclear transfer (SCNT) technique, 23 mammalian species have been successfully cloned, including cattle, cats, dogs, horses and rats. (wikipedia.org)

Thus, telomerase activity and telomere maintenance are associated with the immortality of cancer cells, germ-line cells, and embryonic stem (ES) cells. (nature.com)
These cells are commonly termed embryonic germ (EG) cells because they are derived from primordial germ cells found at the genital ridges in developing fetuses. (biomedcentral.com)
Spermatogonia are undifferentiated male germ cells, or sperm cells. (ussromantics.com)

These cells were then used as donor cells in a process called somatic cell nuclear transfer, where the nucleus of a donor cell carrying the new gene is inserted into an egg cell, known as an ovum, from a female cow. (siasat.com)
It was clear that a definitive experiment required the replacement of a zygote nucleus by a somatic cell nucleus, asking whether the somatic nucleus could functionally replace the zygote nucleus by eliciting normal development of the enucleated recipient egg ( Fig. 1 )? (biologists.com)
Briggs and King ( Briggs and King, 1952 ) had already succeeded in transplanting a blastula cell nucleus into an enucleated egg and obtaining normal tadpoles in the frog Rana pipiens . (biologists.com)

The non-embryonic stem cells like adult stem cells are in clinical use for many years and embryonic stem cells are now emerging as an alternative source for the same purpose with huge potentials in drug discovery and toxicological studies. (benthamscience.com)
Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)
Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)

The vesicular stomatitis pathogen expressing green fluorescent proteins (VSV-GFP) and Vero cell range, a fibroblast-like kidney cell comes from African green monkey, had been supplied by Dr. Markus Czub (College or university of Calgary, Canada). (ap26113.com)
Iron and BIM (Bcl-2-interacting mediator of cell death) proteins were involved in the cytotoxic sensitization process. (jneurosci.org)

These stem cells are considered committed to certain cell lineages. (biomedcentral.com)

EDITOR'S PREFACE: The first paper in this issue of Biology of Reproduction is a minireview entitled "Getting Sperm and Egg Together: Things Conserved and Things Diverged," by Janice P. Evans. (bioone.org)
The minisymposium, organized by Victor D. Vacquier, included the following presentations: "An Hypothesis to Explain the Evolution of Species-Specific Fertilization" by Victor D. Vacquier, "Molecular Biology of the Zona Pellucida: Genetic Mutations and Fertility" by Jurrein Dean, and "Sperm-Egg Membrane Interactions During Fertilizations" by Janice P. Evans. (bioone.org)

The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
conditioned media (cm) from allogeneic stimulated cultures of light density cells (less than 1.08 g/cm3) from the peripheral blood of normal dogs were used to stimulate the growth of erythroid burst-forming units (bfu-e) in bone marrow from normal dogs. (liverpool.ac.uk)
maximum numbers of bfu-e were obtained when 5% (vol/vol) 3 x cm and 2 u/ml erythropoietin were added to plasma clot cultures of bone marrow cells. (liverpool.ac.uk)
We showed that CAMK2G hetero knockout in the primary bone marrow cells expressing MPL W515L decreased colony-forming capacity. (ashpublications.org)

Use of a chronic model of articular cartilage and meniscal injury for the assessment of long-term effects after autologous mesenchymal stromal cell treatment in Steep. (sciendo.com)

A chemical released by cancer cells that induces motility, enabling the cells to metastasize. (unboundmedicine.com)

7 There has been some variation and question regarding the correct terminology for this population of stromal adipose cells. (journalofprolotherapy.com)
Adipose stem cells in reparative goat mastitis mammary gland. (sciendo.com)

2020). This places metabolism at the forefront of development and cell state decisions. (conditionmed.org)
The development of efficient and cost-effective strategies to generate large numbers of functional UCB HSPC ex vivo would boost all current and future medical uses of these cells. (bvsalud.org)
It all begins at the beginning, during the early stages of male foetal development. (ussromantics.com)

pinyin: Huá Huá, born 5 December 2017) are a pair of identical crab-eating macaques (also referred to as cynomolgus monkeys) that were created through somatic cell nuclear transfer (SCNT), the same cloning technique that produced Dolly the sheep in 1996. (wikipedia.org)

and 3) characterization of functional domains in different sperm ligands (fertilin-α and fertilin-β in the mouse and lysin in the abalone) that recognize cognate binding sites on the egg surface. (bioone.org)

Examples of multipotent stem cells are those producing blood, nerve and muscle cells. (biomedcentral.com)
Although several reports indicate an ability of multipotent stem cells to produce cells even outside their normal limits, most of these findings remain highly controversial. (biomedcentral.com)
With high levels of platelet-derived growth factors and cytokines, this combination provides both a living bioscaffold and a multipotent cell replenishment source useful for enhanced musculoskeletal healing. (journalofprolotherapy.com)

We propose that lcn2 is an autocrine mediator of reactive astrocytosis based on the multiple roles of lcn2 in the regulation of cell death, morphology, and migration of astrocytes. (jneurosci.org)
Our results suggest that lcn2 acts in an autocrine manner to induce cell death sensitization and morphological changes in astrocytes under inflammatory conditions and that these phenotypic changes may be the basis of reactive astrocytosis in vivo . (jneurosci.org)

Abstract The candida Ipi3p is required for DNA replication and cell viability in Ipi1-3p have been found to be required for cell viability and for processing of the ITS2 sequences from 35S pre-rRNA in pre-60S ribosomal particles [8, 9]. (irjs.info)

The cells were analyzed by circulation cytometry for monitor cell cycle progression. (irjs.info)

All specialized cells originate from stem cells. (benthamscience.com)

The 30 mg/kg/day time dosage is definitely mid-range between doses (15 and 45 mg/kg) that have previously been shown to be effective in repairing beta cell mass and islet function in two different mouse models of diabetes.19,20 Daily treatments were continued until graft removal at 32C39 days post-transplant. (healthweblognews.info)

But the story has taken a new twist as religious groups are now beginning to discourage followers from participating -- because ALS research at times relies on embryonic stem cells. (salon.com)
this research is funded by one specific donor, who is committed to this area of research. (salon.com)
The Archdiocese suggests people who want to do the challenge contribute to the John Paul II Medical Research Institute, "where the research is only conducted using adult stem cells. (salon.com)
Strategies Animals The precise pathogen free of charge (SPF) eggs had been bought from the Canadian Meals Inspection Company (CFIA), Ottawa, Canada and incubated at medical Research Innovation Middle (HRIC), College or university of Calgary in digital incubators (Rcom Pro 20 & 50, Kingsuromax 20 and Rcom MARU Deluxe utmost, Autoelex Co., Ltd., GimHae, GyeongNam, Korea). (ap26113.com)
Further research revealed CAMK2 γ subtype was important in MF model cells induced by MPL W515L. (ashpublications.org)

Further studies will establish the nature of this novel factor which will enhance the application of this interesting cell type for neural transplantations. (scielo.org.ar)
a type of cancer that originates in the glandular cells, which are responsible for producing and secreting fluids or mucus in various organs of the body. (drpaulkilgore.com)

Anatomy45

Organisms16

Diseases11

Chemicals and Drugs61

Analytical, Diagnostic and Therapeutic Techniques and Equipment36

Phenomena and Processes51

Disciplines and Occupations1

Technology, Industry, Agriculture5

Information Science4

Named Groups4

Health Care3

Embryonic cells1

Bovine4

Embryo16

Vitro7

Differentiation8

Serum2

Pluripotent11

Tissues5

Progenitor cells1

Tissue12

Keratinocytes1

Derive2

Nuclei4

Stromal cells3

Somatic cells4

Organ12

Transplantation9

Mice14

Placenta2

Umbilical3

Human13

Growth Factor3

ESCs2

Amniotic6

Hematopoietic stem1

Placental1

Blastocyst1

Regenerative medicine2

Glial cell1

Differentiate3

Endothelial1

Epithelial cells1

Adult cells1

Stem cell res1

Mammalian1

Germ3

Nucleus3

Clinical3

Proteins2

Lineages1

Biology2

Bone5

Autologous1

Induces1

Adipose2

Development3

19961

Characterization1

Multipotent3

Autocrine2

Viability1

Cytometry1

Originate1

Diabetes1

Research5

Type2