AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation ScienceHealth Care
Cloning, OrganismFibroblastsNuclear Transfer TechniquesFetusEmbryo Culture TechniquesEmbryo TransferCells, CulturedOocytesEmbryo, MammalianBlastocystAnimals, Genetically ModifiedSwineSkinSus scrofaEmbryonic DevelopmentPregnancyGreen Fluorescent ProteinsEmbryonic and Fetal DevelopmentCell LineFibroblast Growth Factor 2TransfectionCattleFibroblast Growth FactorsReceptors, Fibroblast Growth FactorFibroblast Growth Factor 1Receptor, Fibroblast Growth Factor, Type 1Receptor, Fibroblast Growth Factor, Type 2Cell DivisionRNA, MessengerCollagen3T3 CellsReceptor, Fibroblast Growth Factor, Type 3DermisSignal TransductionGene Expression RegulationCell Transformation, NeoplasticMolecular Sequence DataKeloidFibroblast Growth Factor 10Wound HealingExtracellular MatrixFibroblast Growth Factor 7Collagen Type IGrowth SubstancesBase SequenceChick EmbryoCell ProliferationActinsNIH 3T3 CellsCell AgingFibronectinsReceptor, Fibroblast Growth Factor, Type 4Cell DifferentiationLungCell Line, TransformedCell MovementDNAGene ExpressionFibrosisPhosphorylationPlatelet-Derived Growth FactorFibroblast Growth Factor 5Blotting, WesternSynovial MembraneScleroderma, SystemicKineticsFibroblast Growth Factor 4Cell AdhesionCell SurvivalCoculture TechniquesTransforming Growth Factor beta1Culture Media, ConditionedRecombinant ProteinsMutationReverse Transcriptase Polymerase Chain ReactionMatrix Metalloproteinase 1Time FactorsAmino Acid SequenceTranscription, GeneticFluorescent Antibody TechniqueMice, KnockoutPhenotypeUp-RegulationCell Transformation, ViralCell Culture TechniquesEnzyme ActivationImmunohistochemistryApoptosisConnective TissueDose-Response Relationship, DrugInterleukin-1ProcollagenPulmonary FibrosisCell CommunicationFibroblast Growth Factor 8Stromal CellsBlotting, NorthernCell CycleDNA-Binding ProteinsCell Transdifferentiation
Embryonic fibroblastsBovine serumLungCancer-associated fibroblastsTissueVitroChick tendonCAFsCardiacMouseCollagenHumanNeuronsAdultStromalMyofibroblastsShowed myofibroblastMoleculesFunctionalLiverDiagnosisStimulationExosomesEpithelialExpressionFluidProteinCulturesCellularInvasiveFibrosisSignificantlyStudyGenerationSmooth musclSamplesDevelopment
Embryonic fibroblasts4
  • Here we report that TSC1 and TSC2 regulate Smoothened (SMO)-dependent HH signaling in mouse embryonic fibroblasts (MEFs). (springer.com)
  • After ingestion of melanoma-derived exosomes during incubation, mouse embryonic fibroblasts cells emerged cellular invasiveness with the presentation of a marked increase in miR-21 expression. (dovepress.com)
  • STAT5A-Stat5-/- tissue origin: 12.5-13.5-day fetuses cell type: mouse embryonic fibroblasts (MEFs) passages: 9-10 chip antibody: anti-STAT5 chip antibody vendor: Santa Cruz chip antibody cat. (nih.gov)
  • Stat5+/- mice were intercrossed and mouse embryonic fibroblasts (MEFs) were isolated from 12.5-13.5-day fetuses. (nih.gov)
Bovine serum1
  • MEFs were expanded in DMEM with high glucose supplements and 15% fetal bovine serum (FBS). (nih.gov)
Lung1
  • HDCV uses the Pitman Moore strain of fixed rabies virus propagated in infected human diploid cells, and RVA uses a Kissling strain of rabies virus adapted to a diploid cell line of fetal rhesus lung (2,3). (cdc.gov)
Cancer-associated fibroblasts4
  • Cancer‑associated fibroblasts (CAFs) are pivotal in tumor progression. (spandidos-publications.com)
  • The tumor microenvironment consists of various cell types and fibroblasts in the tumor tissue, called cancer-associated fibroblasts (CAFs), which play pivotal role in tumor progression ( 7 , 8 ). (spandidos-publications.com)
  • Understanding the mechanism underlying the role of tumor stroma, especially cancer-associated fibroblasts (CAFs), during chemoresistance would significantly contribute to the clinical application of chemotherapy agents. (dovepress.com)
  • Cancer-associated fibroblasts (CAFs) have been demonstrated to be essential stromal components in the tumor microenvironment, regulating cancer cell growth, migration, invasion, and chemo-response through various mechanisms. (dovepress.com)
Tissue6
  • and colon fibroblasts, CCD‑18Co), resected human tissue samples of colorectal cancers, and immune‑deficient nude mouse xenograft models. (spandidos-publications.com)
  • MiR-21 promoted invasion of fibroblasts by down-regulation of tissue inhibitor of metalloproteinase 3 (TIMP3) expression and increasing of matrix metalloprotein (MMP) expression in fibroblast cells via melanoma-derived exosomes in a time-dependent manner. (dovepress.com)
  • Evaluation of the efficacy of proposed agents in an in vitro model is convenient and allows assessment of the mechanical properties of the bone-tendon interface as well as the effect on fibroblast proliferation and collagen content of the tissue. (researchgate.net)
  • The aim of this study was to evaluate the sealing capability and tissue interaction of mussel-mimetic tissue adhesive (mussel glue) in comparison to fibrin glue on punctured fetal membranes in vivo. (researchgate.net)
  • Using a previously established model of rheumatic scarring / fibrosis in vitro , supported by the evaluation of cord blood from cardiac-NL neonates and their healthy (anti-SSA/Ro-exposed) counterparts, and autopsy tissue from fetuses dying with cardiac-NL, the current study was initiated to more clearly define and distinguish the S100A4-positive fibroblast in the fetal cardiac environment . (bvsalud.org)
  • Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
Vitro6
  • Despite the well-known difficulty in converting adult fibroblasts into functional neurons in vitro, we could detect functional maturation in the induced neurons. (lu.se)
  • Examples of a novel series of phenanthrolinones are shown to be potent competitive inhibitors of avian prolyl 4-hydroxylase, and of collagen hydroxylation, in embryonic chick tendon cells and human foreskin fibroblasts in vitro and in the oestradiol-stimulated rat uterus in vivo. (embl.de)
  • Our studies also show that these NPs have differential requirements for LIF and ciliary neurotrophic factor (CNTF) and for epidermal growth factor (EGF), fibroblast growth factor (FGF-2) and platelet-derived growth factor (PDGF) for their propagation in vitro. (karger.com)
  • In vitro Studies of Protein Transfer Across Human Fetal Membranes. (researchgate.net)
  • Maternal sera were dialyzed in vitro across individual or combined fetal membranes to elucidate the functions of the membranes in protein transport. (researchgate.net)
  • In vitro , fibroblasts cultured with supernatants of macrophages transfected with hY3 (noncoding ssRNA) differentiated into myofibroblasts or S100A4+ fibroblasts . (bvsalud.org)
Chick tendon1
  • bone substitute) and a chick tendon fibroblast-seeded fibrin gel (tendon substitute). (researchgate.net)
CAFs1
  • CAFs and normal fibroblasts differ with respect to the expression of various markers, such as fibrotic markers, growth factors, chemokines and cytokines ( 9 ). (spandidos-publications.com)
Cardiac1
  • These findings position the S100A4+ fibroblast alongside the canonical myofibroblast in the pathogenesis of cardiac-NL. (bvsalud.org)
Mouse8
  • Mouse IL-6 induced by mIL-17A in fetal mouse skin fibroblasts. (biolegend.com)
  • The ED 50 is 0.25 - 1 ng/ml, corresponding to a specific activity 1.0 - 4 x 10 6 units/mg, as determined by a dose dependent stimulation of fetal mouse skin fibroblasts production of IL-6. (biolegend.com)
  • Here we describe a whole organ culture system that allows live imaging of the developing villi in the fetal mouse intestine. (jove.com)
  • Two-photon imaging and 3D reconstruction of the whole fetal mouse intestines in Walton et al . (jove.com)
  • Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. (hindawi.com)
  • Human colorectal cancer (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines were purchased from the American Type Culture Collection (ATCC), USA. (hindawi.com)
  • En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
  • Other cell lines used are Vero and L mouse fibroblasts. (cdc.gov)
Collagen2
Human4
  • Nevertheless, the generation of neurons from adult human fibroblasts, an easily accessible cell source to obtain patient-derived neurons, has proved to be challenging due to the intrinsic blockade of neuronal commitment. (lu.se)
  • In this paper, we used an optimized protocol for adult skin fibroblast reprogramming based on RE1 Silencing Transcription Factor (REST) inhibitn together with a combination of GABAergic fate determinants to convert human adult skin fibroblasts into GABAergic neurons. (lu.se)
  • Generation of two human isogenic iPSC lines from fetal dermal fibroblasts. (mpg.de)
  • The strategy is compared against both manual peak picking and industry standard automated peak picking on a set of mass spectra obtained after tryptic in gel digestion of 2D-gel samples from human fetal fibroblasts. (lu.se)
Neurons2
  • Moreover, we identified specific gene combinations that converted fibroblasts into neurons of a GABAergic intraneuronal fate. (lu.se)
  • Second, we used directly converted neurons from sporadic patient fibroblasts to study of age-related disease relevant pathology. (lu.se)
Adult2
  • In this paper, we used an optimized protocol for adult skin fibroblast reprogramming based on RE1. (lu.se)
  • A). ADAMTS10 expression studies using reverse-transcriptase polymerase chain reaction, northern blot, and dot-blot analyses showed that ADAMTS10 is expressed in skin, fetal chondrocytes, and fetal and adult heart. (nih.gov)
Stromal1
Myofibroblasts1
  • This study addressed fibroblast heterogeneity by examining expression of α- Smooth Muscle Actin ( myofibroblasts ) and of S100 Calcium-Binding Protein A4 (S100A4). (bvsalud.org)
Showed myofibroblast1
  • APJ‑suppressed fibroblasts treated with an antagonist or small interfering RNA showed myofibroblast‑like properties, including increased proliferation and migratory abilities, via accelerated phosphorylation of Sma‑ and Mad‑related protein 2/3 (Smad2/3). (spandidos-publications.com)
Molecules1
  • The researchers found that levels of molecules called fibroblast growth factors (FGFs), and two of the receptors that bind to them, were significantly lower among people who had been diagnosed with severe clinical depression and had died in a depressed state. (biopsychiatry.com)
Functional1
  • Increased appreciation of heterogeneity in fibroblasts encourages re-examination of existing models with the consideration of multiple fibroblast subtypes (and their unique functional differences) in mind. (bvsalud.org)
Liver1
  • Erythropoietin is produced by the liver during fetal life. (medscape.com)
Diagnosis1
  • 1 The Center for Fetal Diagnosis and Treatment, The Children's Hospital of Philadelphia, Philadelphia, USA. (nih.gov)
Stimulation1
  • It was our objective to study the role of mechanical stimulation on fibronectin (FN) reorganization and recruitment by exposing fibroblasts to shear fluid flow and equibiaxial stretch. (nature.com)
Exosomes4
Epithelial1
  • The ability to observe the epithelium three-dimensionally was seminal to demonstrating this point and redefining epithelial morphogenesis in the fetal intestine. (jove.com)
Expression1
  • APJ expression in fibroblasts co‑cultured with p53‑suppressed colon cancer cells (HCT116 sh p53 cells) was significantly lower than in control colon cancer cells (HCT116 sh control cells). (spandidos-publications.com)
Fluid1
  • Complimentary to this, fibroblasts exposed to fluid shear stress structurally rearranged pre-coated surface FN, but unstimulated and stretched cells did not. (nature.com)
Protein3
  • The dogs have an extra copy of the gene that codes for a growth-promoting protein called fibroblast growth factor 4 (FGF4). (nih.gov)
  • This results in overproduction of the FGF4 protein, which the researchers hypothesize may turn on key growth receptors at the wrong times during fetal development. (nih.gov)
  • We identify the main binding sites of a catalytically inactive Cas9 (dCas9) protein in bovine fetal fibroblast cells (BFFs) with chromatin immunoprecipitation sequencing (ChIP-seq). (biomedcentral.com)
Cultures1
  • On the basis of information provided by the manufacturer (6) RabAvertTM is a sterile freeze-dried vaccine obtained by growing the fixed-virus strain Flury low egg passage (LEP) in primary cultures of chicken fibroblasts. (cdc.gov)
Cellular2
  • Fibroblasts are associated with the metastasis of cancer depending on their cellular plasticity. (dovepress.com)
  • Results: No significant effect on cellular proliferation was noted with the addition of AMA to fibroblasts in culture (p = 0.7). (researchgate.net)
Invasive2
  • The invasion analysis showed that exosome-treated fibroblast cells had greater invasive capability as compared to untreated fibroblast cells, with the higher expressions of MMP2 and MMP9. (dovepress.com)
  • Iatrogenic preterm prelabour rupture of fetal membranes (iPPROM) remains the main complication after invasive interventions into the intrauterine cavity. (researchgate.net)
Fibrosis1
  • The apelin‑apelin receptor (APJ) system has been implicated in suppressing fibroblast‑to‑myofibroblast transition in non‑neoplastic organ fibrosis. (spandidos-publications.com)
Significantly1
  • Fetal survival was not significantly lower in any of the treatment groups compared to the negative control. (researchgate.net)
Study3
  • Taken together, our study demonstrated that GO-AgNPs potentially induce oxidative damage to DNA, which result in toxicity and cell apoptosis in caprine fetal fibroblast cell due to an increased generation of ROS. (researchsquare.com)
  • The present study aimed to elucidate the oncogenic role of the apelin‑APJ system in tumor fibroblasts. (spandidos-publications.com)
  • The present study aimed to evaluate the cytotoxicity of different brands of stock acrylic teeth against fibroblast cells. (bvsalud.org)
Generation1
  • Basic fibroblast growth factor (Fgf2) is required for the generation of founder cells within the dorsal pseudostratified ventricular epithelium, which will generate the cerebral cortex, but the ganglionic eminences are not affected. (jneurosci.org)
Smooth muscl1
  • Occasionally antigen was observed in cells that resembled smooth muscle cells, fibroblasts and endothelial cells. (usda.gov)
Samples1
  • Methods 424 fetal samples, sent in for prenatal rasopathy testing in 2011-2016, were collected. (bmj.com)
Development2
  • They have potent effects during embryonic, fetal and child development, and can modify the size and structure of particular brain regions. (biopsychiatry.com)
  • Signs and symptoms of this form can be detected at or before birth and can include impaired growth during fetal development and a small head size ( microcephaly ). (medlineplus.gov)