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  • endothelial cells
  • Endothelial cells participate to synthesize and secrete chemokine and cytokine and are implicated in recruiting immune cells in response to dysfunctions due to different factors (e.g. (frontiersin.org)
  • HemSCs and human umbilical vein endothelial cells (HUVECs) were mixed and injected subcutaneously into 20 flank of BALB/c-nu mice, which were randomly divided into 5 groups based on different E2 treatment doses (0, 0.01, 0.1 and 1 mg, respectively), 0.1 mg dimethyl sulfoxide (DMSO) as control. (dovepress.com)
  • 2002. Fibroblast growth factor-2 induces Lef/Tcf-dependent transcription in human endothelial cells. (meduniwien.ac.at)
  • This proliferative effect of MVECs on human preadipocytes was significantly greater from endothelial cells isolated from adipose tissue than from dermally derived MVECs ( 4 ), suggesting that endothelial cells from human adipose tissue have unique activities necessary for preadipocyte proliferation. (diabetesjournals.org)
  • epidermal
  • A composite living skin equivalent is described comprising an epidermal layer of cultured keratinocyte cells, a layer of non-porous collagen, and a dermal layer of cultured fibroblast cells in a porous cross-linked collagen sponge matrix. (google.es)
  • The present invention relates to living skin equivalents and, in particular, to composite living skin equivalents comprising an epidermal layer of cultured keratinocyte cells, a layer of highly purified, non-porous collagen and a dermal layer of cultured fibroblast cells in a porous, cross-linked collagen sponge. (google.es)
  • Mimicking the topography of the epidermal-dermal interface with elastomer substrates. (hdbr.org)
  • Mimicking the topography of the epidermal-dermal interface with elastomer substrates.Viswanathan P, Guvendiren M, Chua W, Telerman SB, Liakath-Ali K, Burdick JA, Watt FM. (hdbr.org)
  • Among them, epidermal Langerhans cells (LCs), dermal DCs, and macrophages play a key role in the initiation and regulation of immune responses ( 1 ). (rupress.org)
  • At 7 wk EGA, some epidermal cells already express HLA-DR, but not CD1a or Lag antigen, a component of Birbeck granules, which appear at ∼12 wk EGA ( 16 , 19 ). (rupress.org)
  • 2015) Keloid-derived, plasma/fibrin-based skin equivalents generate de novo dermal and epidermal pathology of keloid fibrosis in a mouse model. (chla.org)
  • The model epitomizes the injury-repair environment in triggering and fostering keloid formation and the dynamic epidermal/dermal interactions which occur during disease development. (chla.org)
  • When ready, each strip of foreskin is peeled apart to separate the epidermal top layer from the dermal layer below that. (fourwinds10.com)
  • a) Foreskin or other skin sample is placed epidermal side down (in this case, dark pigmented epidermis) and loose connective tissue is scraped away using a scalpel. (fourwinds10.com)
  • proliferation
  • the cell viability and proliferation were evaluated with the messenger RNA (mRNA) and protein expression levels of fibroblast growth factor 2 (FGF2), vascular endothelial growth factor-A (VEGF-A) and estrogen receptor-α (ER-α), by application of several in vitro assays, such as methyl thiazolyl tetrazolium (MTT), reverse transcriptase-polymerase chain reaction (RT-PCR), real-time PCR, enzyme-linked immunosorbent assay (ELISA) and Western blotting. (dovepress.com)
  • Our hypothesis is that by controlling differential cell proliferation (keratinocyte vs. fibroblast) and cell phenotype, such as integrin and metalloproteinase expression profiles or the magnitude of actin fiber extensions, novel therapies will result that can accelerate the healing of chronic wounds (ulcers) and burns with reduced scarring. (rochester.edu)
  • Reduction in ROS generation endows the transformed cells resistance to As 3+ cytotoxicity, rapid proliferation, anchorage-independent growth, and a sustained activation of the NF-κB transcription factor. (aacrjournals.org)
  • Optimum healing of a cutaneous wound requires a well-orchestrated integration of the complex biological and molecular events of cell migration and proliferation and of extracellular matrix deposition and remodeling. (hindawi.com)
  • Murray E. The Ubiquitin-Proteasome System and Cellular Proliferation and Regulation in Osteoblastic Cells. (patentgenius.com)
  • Fluoride Increase Tyrosine Kinase Activity in Osteoblast-like Cells: Regulatory Role for the Stimulation of Cell Proliferation and Pi Transport Across the Plasma Membrane," J Bone Miner Res (1995) 10:164-171. (patentgenius.com)
  • We have previously reported that MVECs derived from human adipose tissue secrete a factor(s) that stimulates proliferation in human subcutaneous and omental (intraabdominal) preadipocytes ( 4 ). (diabetesjournals.org)
  • dermis
  • Cells from fetal dermis have been proven safe and efficacious in the treatment of pediatric burns, but proper characterization of these cells has not yet been provided. (medindia.net)
  • We generated, propagated and analyzed a proliferating population of cells derived from human fetal dermis taken at 20-22 weeks of gestation," wrote the researchers. (medindia.net)
  • We also found that CD45 + HLA-DR high CD1c + dendritic cells (DCs) are already present in the epidermis and dermis at 9 wk estimated gestational age (EGA) and that transforming growth factor β1 production precedes Langerin and CD1a expression on CD45 + CD1c + Langerhans cell (LC) precursors. (rupress.org)
  • The dermis layer below contains fibroblast cells. (fourwinds10.com)
  • bovine
  • Excellagen® is a flowable dermal matrix in the form of a physiologically formulated homogenate of purified bovine dermal collagen (Type I) in its native 3-dimensional fibrillar configuration, supplied as a sterile professional-use syringe-based product, and capable of providing a structural scaffold for cellular infiltration and wound granulation. (corporate-ir.net)
  • Cells were maintained in DMEM supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1% l -glutamine, and 1% penicillin and streptomycin. (aacrjournals.org)
  • macrophages
  • To date, various blood-borne and skin-resident precursors for LCs, dermal DCs, and macrophages have been described ( 10 - 15 ). (rupress.org)
  • therefore, our knowledge about the ontogeny of dermal DCs, macrophages and other skin leukocytes is scarce ( 17 , 21 ). (rupress.org)
  • Wound Repair
  • Extending from the experience and established work, Dr. Tuan has taken a focused effort in the past several years to generate a clinically relevant and deliverable keloid-derived, plasma/fibrin-based skin equivalent animal model of keloid fibrosis that bridges the major gap of keloid translational research (Wound Repair Regen. (chla.org)
  • despite
  • Despite extensive efforts in determining how As 3+ causes malignant transformation of the normal cells in a number of experimental systems, the carcinogenic mechanism of As 3+ remains poorly understood. (aacrjournals.org)
  • The properties and molecular hallmarks of these cells are not well understood, despite their pivotal role in cancer etiology and resistance to treatment. (biomedcentral.com)
  • Despite decades of research, there are currently no effective treatments for advanced liver disease and a liver transplantation is the only treatment option for patients. (ku.edu)
  • capillaries
  • It also allows nutrients and systemic factors to reach the whole skin tissue throughout the skin microvascular network from arteries to small capillaries close to the epidermis. (frontiersin.org)
  • Histological examinations were performed to evaluate the densities of GFP+ cells and capillaries. (plos.org)
  • Increased numbers of GFP+ cells and capillaries were observed in group 2. (plos.org)
  • Skin maintains homeostasis by temperature regulation via hair follicles, sweat glands and dermal capillaries, and by lubrication via sebaceous glands. (mdpi.com)
  • The pink skin is devoid of pigment cells (melanocytes), and appears pink from the underlying network of capillaries. (wikipedia.org)
  • Expansion of adipose tissue in obesity is preceded by the development of new capillaries ( 1 ). (diabetesjournals.org)
  • somatic
  • NT-ESCs displayed normal diploid karyotypes and inherited their nuclear genome exclusively from parental somatic cells. (pubmedcentralcanada.ca)
  • Cytoplasmic factors present in mature, metaphase II (MII)-arrested oocytes have a unique ability to reset the identity of transplanted somatic cell nuclei to the embryonic state. (pubmedcentralcanada.ca)
  • However, when a somatic cell nucleus is transplanted into an intact oocyte containing its own chromosomes, the resulting polyploid embryos are able to develop to blastocysts and support ESC derivation. (pubmedcentralcanada.ca)
  • tumor
  • Each group of mice were treated intramuscularly every week, then 2 and 4 weeks later, the subcutaneous implants were harvested and evaluated the tumor tissues with microvessel density (MVD) assay and immunohistochemistry. (dovepress.com)
  • Paradoxically, generation of ROS is also the one of the central mechanisms to the desired tumor cell apoptosis induced by As 3+ and some chemotherapeutic reagents. (aacrjournals.org)
  • Tumor-initiating cells (TIC) are being extensively studied for their role in tumor etiology, maintenance and resistance to treatment. (biomedcentral.com)
  • Transduction of OCT4 in normal breast preparations led to the generation of cell lines possessing tumor-initiating and colonization capabilities. (biomedcentral.com)
  • Within this hierarchy, a rare population of undifferentiated cells is able to self-renew, proliferate, and develop into more differentiated tumor cells. (biomedcentral.com)
  • However, the isolation of TICs has been hampered because these cells represent a rare population within the tumor, making it difficult to study their role in tumor biology. (biomedcentral.com)
  • These approaches ultimately will facilitate the potential discovery of targeted therapeutics that are specific for tumor cell initiation. (biomedcentral.com)
  • skin
  • The researchers noted that the most interesting aspect of their study was the finding that multipotent cells can be successfully isolated from small fetal skin biopsies and maintained in culture for long periods with multipotency, stability and low immunogenicity retained, "thus generating large quantities of cells for clinical use. (medindia.net)
  • 10. The composite living skin equivalent of claim 9, wherein the fibroblast cells are obtained from treating a dermal sample with collagenase. (google.es)
  • 2018. Development and characterisation of a human chronic skin wound cell line - towards an alternative for animal experimentation . (cardiff.ac.uk)
  • The skin is a highly active immunological organ that contains a multitude of specialized immune cells. (rupress.org)
  • During skin development, LC, dermal DC, and macrophage precursors are believed to colonize the embryonic skin, showing a primitive surface marker pattern that subsequently develops into the profile of resident cells found in adult skin ( 16 , 17 ). (rupress.org)
  • HLA-DR + cells have been described in skin at 7 wk EGA. (rupress.org)
  • The overall research area in the laboratory is the creation of Smart Bandage Biomaterial Engineering and Skin Disease. (rochester.edu)
  • In this study, we propose a novel skin patch that combines antibiotic, cell-derived extracellular matrix (ECM) and biocompatible polyvinyl alcohol (PVA) hydrogel. (thno.org)
  • In fact, high basal HA levels and sustained HA induction in fetal skin are associated with the scarless wound healing that occurs after injury. (ku.edu)
  • The technique has immediate and important implications for developing new cell-based treatments for skin diseases such as vitiligo, as well as new screening strategies for melanoma. (technologynetworks.com)
  • Xu and his colleagues anticipate using their new technique in the treatment of a wide variety of skin diseases, particularly those such as vitiligo for which cell-based therapies are the best and most efficient approach. (technologynetworks.com)
  • An unusual autoimmune disease that causes skin and lung damage can be treated effectively by stem cell transplant, a new study found. (technologynetworks.com)
  • 1996. Human delayed type hypersensitivity reaction in a SCID mouse engrafted with human T cells and autologous skin. (meduniwien.ac.at)
  • The advantages of tissue-engineered skin replacements (cellular or acellular biometrices) greatly improved the therapeutic potential for chronic wounds. (hindawi.com)
  • Wounds are the result of injuries to the skin that disrupt the other soft tissue. (hindawi.com)
  • bone
  • Bone marrow biopsy and aspiration is the removal of some bone marrow tissue for diagnosis and management of cancers of blood cells and multiple myeloma. (medindia.net)
  • In this stem cell from bone marrow are injected into a recipient after treating them with growth factor. (medindia.net)
  • Because MSCs' stemness and immune properties appear to be more robustly expressed and functional which are more comparable with fetal than adult-derived MSCs, MSCs harvested from the "young" WJ are considered much more proliferative, immunosuppressive, and even therapeutically active stem cells than those isolated from older, adult tissue sources such as the bone marrow or adipose. (mdpi.com)
  • For example, the defining test for bone marrow or hematopoietic stem cells (HSCs) is the ability to transplant the cells and save an individual without HSCs. (euvolution.com)
  • Ozaki K. NF-kB Inhibitors Stimulate Apoptosis of Rabbit Mature Osteoclasts and Inhibit Bone Resorption by These Cells. (patentgenius.com)
  • Ferretti, J., "Perspectives of pQct Technology Associated To Biomechanical Studies in Skeletal Research Employing Rat Models," Bone (1995) 17:353S-364S. (patentgenius.com)
  • Hematopoietic
  • Preferred Term is Hematopoietic stem cell transplantation. (medindia.net)
  • A unifying feature is their hematopoietic origin and expression of HLA-DR. LCs and dermal DCs are professional APCs that express high levels of HLA-DR and CD1c ( 2 ). (rupress.org)
  • clinical
  • Their research provided a protocol for the isolation and expansion of large numbers of MFDCs that may see future clinical use, said the study authors. (medindia.net)
  • SAN DIEGO, CA - May 12, 2014 - Taxus Cardium Pharmaceuticals Group Inc. (Trading Symbol: CRXM) today announced that the Company's Excellagen® flowable dermal matrix in combination with Orbsen Therapeutics' mesenchymal stromal stem cell therapy Cyndacel-M™ has been selected for clinical evaluation in a Phase 1b safety study for the potential treatment of chronic diabetic wounds to be funded by the European Union under EU Framework 7 (FP7). (corporate-ir.net)
  • In the initial phase of the project, academic and clinical research teams in Galway, Berlin, Belfast, Munich and Porto tested Orbsen Therapeutics' stem cell product (called Cyndacel-M™) against the current standard cell therapy. (corporate-ir.net)
  • An independent panel at the Steno Diabetes Centre in Copenhagen decided to use Cyndacel-M™ for a clinical study in patients with chronic diabetic ulcers, in preference to the current cell technology. (corporate-ir.net)
  • The Cyndacel-M™ stem cell product candidate consists of a population of highly characterized stromal cells and is designed to provide improved clinical safety and efficacy compared to other cell-based therapies. (corporate-ir.net)
  • We are pleased to have Excellagen® be selected for this stem cell based clinical study, and believe it could potentially be used to enable and enhance the effectiveness of a variety of stem cell and other clinical applications," stated Christopher J. Reinhard, Chief Executive Officer of Taxus Cardium. (corporate-ir.net)
  • Cell culture is one of the most critical bioprocesses for scientific and clinical purposes. (hindawi.com)
  • Furthermore, especially in clinical cell processing for cell-based therapy, manual procedures require a highly experienced staff [ 2 ], leading to higher therapeutic costs and thus preventing the widespread use of cell-based therapy [ 3 ]. (hindawi.com)
  • Additional studies are warranted to translate the use of WJ-derived stem cells for clinical applications. (mdpi.com)
  • With stem cell therapies, medicine is learning how to tap into such cell specialization for new clinical treatments. (technologynetworks.com)
  • This vaccine is being created using foreskin cells and clinical trials have already begun. (fourwinds10.com)
  • granulation tissue
  • Excellagen® can activate platelets, triggering release of essential growth factors and is believed to function as an acellular biological modulator to activate the wound healing process and significantly accelerate the growth of granulation tissue. (corporate-ir.net)
  • stromal
  • Wharton's jelly (WJ) is a gelatinous tissue within the umbilical cord that contains myofibroblast-like stromal cells. (mdpi.com)
  • A unique cell population of WJ that has been suggested as displaying the stemness phenotype is the mesenchymal stromal cells (MSCs). (mdpi.com)
  • Increased matrix metalloproteinase (MMP) synthesis in response to ciprofloxacin treatment has been reported in several cell types, including tenocytes ( 7 - 10 ), chondrocytes ( 11 ), corneal epithelial cells and corneal stromal keratocytes ( 4 ). (spandidos-publications.com)
  • wounds
  • Excellagen® is indicated for the treatment of hard to heal wounds such as diabetic foot ulcers and pressure ulcers as well as other dermal wounds. (corporate-ir.net)
  • Eventually, wounds recover structural integrity and normal functions as a result of spatio-temporally controlled interactions among cells, growth factors, and extracellular matrix (ECM). (thno.org)
  • Furthermore, modern strategies in current tissue engineering (TE) applications can provide promising solutions for chronic wounds management. (hindawi.com)
  • Exogenous Transforming Growth Factor-Beta 2 Enhances Connective Tissue Formation and Wound Strength in Guinea Pig Dermal Wounds Healing by Secondary Intent," Ann Surg (1990) 211(3):288-294. (patentgenius.com)
  • novel
  • We provide tools for path-breaking research by developing antibodies that detect a comprehensive library of novel and established targets. (abgent.com)
  • Although MSCs have been harvested from many different tissues, novel considerations of tissue specificity may dictate the eventual fate of MSCs. (mdpi.com)
  • We therefore suggest that FGF-1 is a key human adipogenic factor, and these data expand our understanding of human fat tissue growth and have significant potential for development of novel therapeutic strategies in the prevention and management of human obesity. (diabetesjournals.org)
  • hypothesis
  • Our hypothesis is that adipose-derived stem cells can be directed toward differing phenotypes simultaneously by simply co-culturing them in bilayered matrices 1 . (jove.com)
  • chronic
  • Therefore, I chose to focus my dissertation research on exploring hepatic wound healing after acute and chronic liver injury. (ku.edu)
  • Accumulating evidence demonstrates that WJ-derived stem cells possess many potential advantages as transplantable cells for treatment of various diseases (e.g., cancer, chronic liver disease, cardiovascular diseases, nerve, cartilage and tendon injury). (mdpi.com)
  • mice
  • 50 dB/db mice were employed and randomly assigned into five groups with 10 mice in each: group 1 (untreated cell), group 2 (PHD2 silenced cell), group 3 (L-mimosine treated cells), group 4 (nontargeting siRNA treated cells) and group 5 (sham control). (plos.org)
  • Ten days postoperatively, 3 mice from each group were sacrificed and wound tissues were harvested. (plos.org)
  • These cells developed high-grade, poorly differentiated breast carcinomas in nude mice. (biomedcentral.com)
  • interactions
  • Dr. Tuan received her postdoctoral training in the area of Cell-ECM interactions from Dr. Fred Grinnell at the University of Texas Southwestern Medical Center at Dallas. (chla.org)
  • 1997. Endothelial cell tube formation depends on cadherin-5 and CD31 interactions with filamentous actin. (meduniwien.ac.at)
  • molecular
  • The isolation of TICs has been limited by the scarcity of this population in the tissue of origin and because the molecular signatures that characterize these cells are not well understood. (biomedcentral.com)
  • Healing
  • Our laboratory is focused on dermal wound healing. (jove.com)
  • This laboratory investigates the fundamental optical, morphological, and surface chemical properties of bioengineered nanoporous silicon (PSi) in developing a platform of Smart Bandage technologies targeting biosensors for point of care diagnosis of cutaneous disease, transdermal drug delivery, and tissue engineering for wound healing. (rochester.edu)
  • The combination of Cyndacel-M™ and Excellagen® improved wound closure and neo-vascularization in a diabetic dermal wound healing model. (corporate-ir.net)
  • The aim of this review is to describe well established characteristics of stem cells and to delineate new advances in the stem cell field, in the context of burn injury and wound healing. (mdpi.com)
  • Healing of a wound is a complex and protracted process of tissue repair and remodeling in response to injury. (hindawi.com)
  • Wound healing is the interaction of a complex cascade of cellular and biochemical actions leading to the restoration of structural and functional integrity with regain of strength of injured tissues. (hindawi.com)
  • characteristics
  • The present review discusses the phenotypic characteristics, therapeutic applications, and optimization of experimental protocols for WJ-derived stem cells. (mdpi.com)
  • Transmission electron micrograph of an adult stem cell displaying typical ultrastructural characteristics. (wikipedia.org)