Fetal cells adult cells clone cells. Medical search. Frequent questions

And now Washington joins the infamous list with Senate Bill 5594, a thoroughly disingenuous piece of legislation that purports to outlaw the cloning of human beings, but by manipulating language and redefining terms, actually permits human cloning and gestation of the resulting cloned embryos through the ninth month. (cbc-network.org)
I've been working with mammalian embryos for over 40 years, with some work in my lab specifically focusing on various methods of cloning cattle and other livestock species. (wptv.com)
Sometimes the process of cloning by somatic cell nuclear transfer still produces abnormal embryos, most of which die. (wptv.com)
A year ago, we showed that you could do it with cells from embryos," says Wilmut. (newscientist.com)
Particularly valuable animals could be cloned from adult cells without the uncertainties of crossing them with other animals or tinkering with embryos. (newscientist.com)
More importantly, biotechnologists will for the first time be able to manipulate the genes of cells from farm animals directly before growing them into embryos. (newscientist.com)
But scientists have not managed to isolate such cells from farm animals, and must rely instead on injecting genes randomly into early embryos. (newscientist.com)
The somatic cell and the oocyte is then fused (f) and the embryos is allowed to develop to a blastocyst in vitro (g). (biomedcentral.com)
Currently, the efficiency for nuclear transfer is between 0-10%, i.e., 0-10 live births after transfer of 100 cloned embryos. (biomedcentral.com)
Citizens disagree about whether we should destroy human embryos for their stem cells-and if so, which embryos, with whose money, under what regulatory guidelines. (eppc.org)
The holy grail of regenerative medicine-whatever one's ethical beliefs about destroying embryos-is to "reprogram" regular cells from one's own body so that individuals can be the source of their own rejection-proof therapies. (eppc.org)
That is to say, we risk turning developed cells into developing embryos, and thus risk engaging in the very activities of embryo destruction and human cloning that we seek to avoid. (eppc.org)
Far more controversial-and for good reason-are stem cells derived from destroyed human embryos. (eppc.org)
Pro-cloning forces have been working hard to convince state governments to pass constitutional amendments enshrining a "right" to clone and to destroy embryos for research. (flfamily.org)
The stem cells suits human needs, does not cause harm and can be obtained from both adult and fetal does not conflict with religious beliefs, it has tissues, umbilical cord and early embryos. (who.int)
Unicellular for those cells that are derived from human organisms are primed to replicate (clone) pre-embryos, which seem to have a high themselves by nature. (who.int)
First, while stem-cell experimentation could involve the creation of embryos with the express purpose of destroying them, this is not the only means available for obtaining embryos. (jewishvaluesonline.org)
Thus, there is broad halakhic (Jewish legal) agreement that stem cell research is permitted on "excess" embryos. (jewishvaluesonline.org)
Most (but not all) authorities would forbid the creation of embryos with the express purpose of killing them in the pursuit of stem cell research. (jewishvaluesonline.org)
The controversy over stem cell research is focused specifically on the use of stem cells taken from embryos. (jewishvaluesonline.org)
In a 2012 letter to Children of God for Life, PepsiCo stated that "Senomyx does not use HEK cells or any other tissues or cell lines derived from human embryos or babies for research performed on behalf of PepsiCo. (hli.org)

A little shot of electricity comes next, and if all goes well, a new human cloned embryo comes into being and begins to develop in the same way as a sexually created embryo. (cbc-network.org)
Cloning of a human being" means asexual reproduction by implanting or attempting to implant the product of nuclear transplantation [e.g., an embryo] into a uterus or substitute for a uterus with the purpose of producing a human being. (cbc-network.org)
Fertilization of mammalian eggs is followed by successive cell divisions and progressive differentiation, first into the early embryo and subsequently into all of the cell types that make up the adult animal. (todayinsci.com)
The first offspring to develop from a differentiated cell were born after nuclear transfer from an embryo-derived cell line that had been induced to became quiescent. (todayinsci.com)
Using the same procedure, we now report the birth of live lambs from three new cell populations established from adult mammary gland, fetus and embryo. (todayinsci.com)
When the one-cell embryo duplicates its genetic material, both cells of the now two-cell embryo are genetically identical. (wptv.com)
But there was no way to easily know all the characteristics of the animal that would result from a cloned embryo or fetus. (wptv.com)
During the development of vertebrates, including humans, the fertilized egg develops into the embryo, and the cells in the embryo then proceed to differentiate to form somatic cells of different tissues and organs. (shawprize.org)
The fertilized egg is considered totipotent, as it can develop into a whole organism, while the cells in the embryo are pluripotent because they are capable of differentiating into somatic cells that make up all the organs. (shawprize.org)
They pioneered a new technique of starving embryo cells before transferring their nucleus to fertilized egg cells. (shawprize.org)
They produced idential lambs called Megan and Morag, which originated from different cells of the same embryo. (newscientist.com)
The latest experiments have also produced three lambs from the cells of a sheep fetus aborted after 26 days, and four from a nine-day-old embryo. (newscientist.com)
After growing and dividing for a week or so in a laboratory culture dish, the fused cell forms an early embryo called a blastocyst, which Wilmut's team implants into a surrogate mother. (newscientist.com)
Although the efficiency of nuclear transfer has been dramatically improved from the initial success rate of one live clone born from 277 embryo transfers [ 1 ], none of the aforementioned efforts abolished the common problems associated with nuclear transfer. (biomedcentral.com)
Research advocates attack President Bush for "banning stem cell research," while pro-life advocates lament a Republican administration and Congress that have banned nothing-not embryo destruction, not human cloning, not fetal farming, not genetic engineering. (eppc.org)
Before leaving office, President Clinton sought to get around the existing law without actually changing it, by funding research on embryonic stem cells so long as the actual embryo destruction was paid for with private dollars. (eppc.org)
Nor is the embryo just a "fertilized egg", or just a "clump of cells", or appear only when the zygote is formed, or appear later after the zygote is formed, or appear after implantation - or even a week after that at 14-days. (lifeissues.net)
Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
In the first 4 - 5 days after fertilization, the early-stage embryo (or blastocyst) is comprised of about 150 cells, within which there is a region called the Inner Cell Mass containing the stem cells. (jewishvaluesonline.org)
The controversy arises for some people because, in the course of harvesting these cells, the embryo is destroyed. (jewishvaluesonline.org)
increased public sensitivity and awareness together with the development of national regulations of governance of human cloning and embryo research in general. (lifeissues.net)
3. National regulations of governance of human cloning and embryo research in general adopted so far confirm the convergence of views of the refusal to adopt legislation or guidelines permitting reproductive cloning , while they still show variations on the legitimacy of human cloning carried out as part of research agendas. (lifeissues.net)

Equine mesenchymal stem cells from bonemarrow, adipose tissue and umbilical cord:immunophenotypic characterization anddifferentiation potential. (sciendo.com)
Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia. (sciendo.com)
Adipose Tissue- and Bone Marrow-Derived Mesenchymal Stem Cells from Sheep: Culture Characteristics. (sciendo.com)
What was special about Dolly is that her "parents" were actually a single cell originating from mammary tissue of an adult ewe. (wptv.com)
She is the first mammal ever created from the non-reproductive tissue of an adult animal. (newscientist.com)
If the same could be achieved in humans, it would mean that each of us could have clones of ourselves made from our own tissue. (newscientist.com)
Mammary glands are rich in these cells, which are more adaptable than other tissue. (newscientist.com)
But if, as seems likely, the Roslin team has succeeded in making an entire animal from adult tissue, it might be possible to do the same for humans. (newscientist.com)
and altering cell and tissue characteristics for biomedical research and manufacturing. (nationalacademies.org)
Fetal Tissue Research: Antiquated and Unethical? (flfamily.org)
Policymakers have the opportunity to hear expert witnesses attest to amazing scientific advancements and discoveries using ethical alternatives to fetal tissue. (flfamily.org)
One of the greatest controversies triggered tissue, a stem cell encoding for heart tissue by the rapid pace of evolution in biology, will eventually develop into heart tissue particularly in genomics and biotechnology, and so on. (who.int)
Using probes modeled after species conserved domains of heparan sulfate proteoglycans, we cloned a novel gene called novocan, raised monoclonal antibodies against a segment of the predicted amino acid sequence of the expressed protein (NOVOcan) and used the antibodies to establish the cell and tissue localization of NOVOcan in postnatal rat brains by immunohistochemistry. (ox.ac.uk)
Speed while performing the procedure helped, they learned, and scientists discovered clones created out of cells from fetal tissue did better than when they used adult cells. (cmaaa.co.za)
Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
For instance, it may be possible one day to produce cardiac tissue to repair a heart damaged in a heart attack, nerve tissue to repair spinal cord injuries and cell therapies to treat people suffering from Alzheimer's or ALS. (jewishvaluesonline.org)
Recent research has shown that pieces of fetal nigral tissue placed in the striatum of 6-OHDA lesioned rats offer greater cell survival and predictability of graft function (in comparison to dissociated nigral cells) in the animal model of Parkinson's disease [ 20 ]. (hindawi.com)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)

If the cloned human organism is to be experimented upon and destroyed, the process is often called "therapeutic cloning. (cbc-network.org)
Since then, the work of Wilmut and Campbell has been duplicated in many other animal species and has provided approaches to produce useful therapeutic products with cloned animals and to improve agricultural practices. (shawprize.org)
Hematopoietic stem cell transplantation remains the only curative therapeutic approach that reliably results in resolution of bone marrow fibrosis in patients with myelofibrosis. (haematologica.org)
But adult stem cells also raise some interesting ethical dilemmas alongside their great therapeutic promise. (eppc.org)
The heated debate in our society over reproductive cloning, as well as therapeutic cloning to obtain embryonic stem cells, has been fueled by misconceptions and hyperbole on both sides. (flfamily.org)
Therapeutic cloning possesses enormous potential for revolutionizing medical and thera- peutic techniques. (who.int)
This is therapeutic cloning. (who.int)
This cell then has therapeutic cloning: the global the capacity to divide and grow into an exact replica of the original from whom the debate somatic cell was taken. (who.int)
The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
In particular, scientific developments in areas such as iPS cells open new possibilities of research and, at mid term, of therapeutic applications, but they also bring new ethical challenges and problems requiring further reflection and debate. (lifeissues.net)
Transplantation of immature cells has been considered a potential therapeutic strategy for the damaged adult brain and spinal cord, and there is currently sustained interest in the generation of stem cell lines that could be used to treat certain CNS injuries or disorders. (hindawi.com)
Since the introduction of the anthracyclines (daunorubicin, idarubicin) and cytarabine, these therapeutic agents have been the cornerstones of remission induction therapy for adult AML. (ashpublications.org)
With this background information as a foundation, we then discuss each of the key questions in relation to the upcoming therapeutic trial and critically assess if the time is ripe for clinical translation of parthenogenetic stem cell technology in Parkinson's disease. (lu.se)

The bill purports to promote stem-cell research, while outlawing the cloning of a human being. (cbc-network.org)
While stem-cell research holds enormous potential for treating or even curing some diseases, the cloning of a human being is morally and ethically unacceptable…Any attempt to clone a human being is in direct conflict with the public policies of this state. (cbc-network.org)
When People Say: "Embryonic stem cell research is. (christianliferesources.com)
Dolly was an important milestone, inspiring scientists to continue improving cloning technology as well as to pursue new concepts in stem cell research. (wptv.com)
These moral perils are surely not a reason to oppose adult stem cell research, which deserves vigorous and expanded public support. (eppc.org)
Last year, states considered more than 170 bills on embryonic and adult stem cell research. (consciencelaws.org)
Should embryonic stem cell research be legal? (consciencelaws.org)
Should the state fund adult stem cell research instead? (consciencelaws.org)
What is the Jewish perspective on stem cell research? (jewishvaluesonline.org)
If embryonic stem-cell research offers real possibilities for future cures then, from a Jewish point of view, it may be pursued with caution, humility, and strict supervision. (jewishvaluesonline.org)
At International Society for Stem Cell Research-the big stem cell meeting-Yamanaka revealed one more factor. (medscape.com)

If it is to be brought to birth, the process is usually called "reproductive cloning. (cbc-network.org)
Last year they used the same reproductive technology to create the world's first cloned lambs (Nature, vol 380, p 64). (newscientist.com)
But we can only wonder about the ethical propriety of producing the first human child with this technique, knowing that the hoped-for newborn would be a reproductive experiment, one that may end initially in numerous fetal failures. (eppc.org)
Cloning technology, however, is perceived as having the potential for reproductive cloning, which raises serious ethical and moral concerns. (who.int)
Reproductive cloning versus germ cell (egg, ovum). (who.int)
But what is not getting such wide reporting is the use of pluripotent stem cells (as well as many other types of cells and genetic engineering techniques) for reproductive purposes . (lifeissues.net)

English embryologist who in 1996 supervised the team of scientists that produced a lamb named Dolly, the first mammal cloned from a cell from an adult. (todayinsci.com)
It's been 20 years since scientists in Scotland told the world about Dolly the sheep , the first mammal successfully cloned from an adult body cell. (wptv.com)
Dolly was an exact genetic copy of that sheep - a clone. (wptv.com)
In fact, one of the coauthors of the paper announcing Dolly worked in our laboratory for three years prior to going to Scotland to help create the famous clone. (wptv.com)
In contrast, Dolly was produced by what's called somatic cell nuclear transfer. (wptv.com)
Dolly was the culmination of hundreds of cloning experiments that, for example, showed diploid embryonic and fetal cells could be parents of offspring. (wptv.com)
Dolly demonstrated that adult somatic cells also could be used as parents. (wptv.com)
By my calculations, Dolly was the single success from 277 tries at somatic cell nuclear transfer. (wptv.com)
One of the live-born lambs, Dolly, was derived from the transplantation of the nucleus of an adult mammary cell. (shawprize.org)
Thus, Dolly was the first example of the reprogramming of the adult cell back to totipotency in a mammal. (shawprize.org)
The difference with Dolly is that all her DNA originated in a cell from the udder of an adult sheep. (newscientist.com)
Dolly was the only lamb born from 277 fusions of oocytes with udder cells. (newscientist.com)
is a British developmental biologist who was the first to use nuclear transfer of differentiated adult cells to generate a mammalian clone, a Finn Dorset sheep named Dolly, born in 1996. (mathisfunforum.com)
CNN)For the first time, scientists say they created cloned primates using the same complicated cloning technique that made Dolly the sheep in 1996. (cmaaa.co.za)
In the case of Hua Hua and Zhong Zhong, researchers used modern technology developed only in the last couple of years to enhance the technique used to clone Dolly, which is called somatic cell transfer, or SCNT. (cmaaa.co.za)
When scientists made Dolly the sheep, years after she was born they used the same cell cluster to make four other sheep clones. (cmaaa.co.za)
The inventors on the '233 application, Dr. Keith H.S. Campbell and Sir Ian Wilmut, were the first to produce a cloned mammal from an adult somatic cell -- Dolly the sheep. (patentdocs.org)
Dr. Campbell and Sir Ian obtained U.S. Patent No. 7,514,258 for the method they used to produce Dolly: somatic cell nuclear transfer, which involves removing the nucleus of a somatic cell that has been arrested in the quiescent phase of the cell cycle and implanting that nucleus into an enucleated oocyte. (patentdocs.org)
On appeal, the Roslin Institute argued that unlike the donor sheep used to create Dolly, clones like Dolly are eligible for protection because they are 'the product of human ingenuity' and 'not nature's handiwork, but [their] own. (patentdocs.org)

Viable Offspring Derived from Petal and Adult Mammalian Cells', Nature (1997), 385 , 810. (todayinsci.com)
Attempts were then made to show that mammalian cells - and human cells in particular - could also be reprogrammed back to a pluripotent state, because it is believed that such knowledge may advance our understanding of developmental mechanisms, and yield new approaches for disease treatment. (shawprize.org)
Viable Offspring Derived from Fetal and Adult Mammalian Cells' (1997), by Ian Wilmut et al. (asu.edu)
Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. (biomedcentral.com)
Though functionally well-characterised in vitro and utilizing the mammalian cell fashions, much less is thought in regards to the molecular mechanisms of its involvement within the signaling and metabolic pathways, which mediate its mobile features. (rbprotein.com)
Destruction or damage to neural circuits in the adult mammalian central nervous system (CNS) is notoriously difficult to repair. (hindawi.com)
The sphingomyelin that accumulates in the lysosomes of Niemann-Pick disease-affected cells is thought to arise from the degradation of the cells and their organelles because it is a major component of all mammalian cell membranes. (medscape.com)

Comparison of human mesenchymal stromal cells from four neonatal tissues: Amniotic membrane, chorionic membrane, placental decidua and umbilical cord. (sciendo.com)
Today, we can derive stem cells from a range of adult and newborn tissues: liver cells, kidney cells, brain cells, fat cells, and umbilical cord blood. (eppc.org)
In the normal course of gestation, these cells will divide and split off from one another to become every cell in the human body, forming the various organs and tissues. (jewishvaluesonline.org)
Its clinical presentation clearly differs from other forms of GSD, because it is caused by the deficiency of the lysosomal enzyme, alpha-1,4-glucosidase, leading to the pathologic accumulation of normally structured glycogen within the lysosomes of most tissues, differs Three forms of the disease exist: infantile-onset, late-onset juvenile and adult onset. (medscape.com)

164. The clone of any of claims 155-159, wherein the donor mammal is non-foetal. (patentdocs.org)

Through simply changing cell culture conditions, a newly established cell line, Erythroid Line from Lund University (ELLU), is able to differentiate toward mature cells, including enucleated reticulocytes. (lu.se)
ELLU is heterogeneous and, unexpectedly, clones expressing adult hemoglobin rapidly differentiate and produce fragile cells. (lu.se)
Myogenesis is a multi-step process through which uncommitted mesodermal cells commit, differentiate and undergo profound phenotypic changes. (biologists.com)
The have been applied to both the plant and ani- stem cells possess pluripotential charac- mal kingdoms without even stirring a ripple teristics, and can differentiate into various of concern in international conscience [ 2 ]. (who.int)
Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)

The scientists honoured by the 2008 Shaw Prize in Life Science and Medicine used different approaches to reprogramme an adult cell into the totipotent or pluripotent state, and in doing so made important contributions to potential new approaches to improve agriculture practices and to treat human diseases. (shawprize.org)
Human pluripotent stem cells can be derived from somatic cells by forced expression of defined factors, and more recently by nuclear-transfer into human oocytes, revitalizing a debate on whether one reprogramming approach might be advantageous over the other. (nih.gov)
Here we compared the genetic and epigenetic stability of human nuclear-transfer embryonic stem cell (NT-ESC) lines and isogenic induced pluripotent stem cell (iPSC) lines, derived from the same somatic cell cultures of fetal, neonatal and adult origin. (nih.gov)
In addition, the possibility of reprogramming adult stem cells back to a "pluripotent" (or embryonic-like) state raises the biological prospect of going back too far. (eppc.org)
Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
Chad and I have both been principal investigators on the induced pluripotent stem cell (IPSC) grant. (medscape.com)
I fell in love with the idea of human pluripotent stem cells and started surveying the United States and even Europe for who was playing in that area. (medscape.com)

The potential of intra-articular injection of chondrogenic-induced bone marrow stem cells to retard the progression of osteoarthritis in a sheep model. (sciendo.com)
She lived to six and a half years, when she was eventually put down after a contagious disease spread through her flock, infecting cloned and normally reproduced sheep alike. (wptv.com)
They performed nuclear transfer experiments in which nuclei from embryonic, foetal and adult cells of the sheep were transplanted into fertilized eggs derived from ewes. (shawprize.org)
They further created a sheep called Polly in which they showed that it was possible to incorporate a human gene into the donor's DNA before cloning, thus indicating that it may be possible to use animals to produce human proteins for the benefit of mankind. (shawprize.org)

In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
2017). MMP-2 and MMP-14 Silencing Inhibits VEGFR2 Cleavage and Induces the Differentiation of Porcine Adipose-Derived Mesenchymal Stem Cells to Endothelial Cells. (sciendo.com)
Bovine fetal mesenchymal stem cells exert antiproliferative efect against mastitis causing pathogen Staphylococcus aureus. (sciendo.com)
Cryopreserved allogeneic mesenchymal stem cells enhance wound repair in full thickness skin wound model and cattle clinical teat injuries. (sciendo.com)

It is the policy of Washington state that research involving the derivation and use of human embryonic stem cells, human embryonic germ cells, and human adult stem cells from any source, including somatic cell nuclear transplantation , is permitted upon full consideration of the ethical and medical implications of this research. (cbc-network.org)
Long before the controversy emerged over human embryonic stem cells, scientists and doctors began using first-generation stem cells from adult bone marrow. (eppc.org)

These germ cells are the only ones in the body that have their genetic material all jumbled up and in half the quantity of every other kind of cell. (wptv.com)
This paper outlines the debates prompted through a reproduction mechanism involv- by progress in cloning research, with special ing male and female germ cells. (who.int)

The technique synchronized the cell cycles of both cells and the results led Wilmut and Campbell to believe that any type of cell could be used to produce a clone. (shawprize.org)
Wilmut and his colleagues fuse the empty oocyte with the donor cell by bringing them together and subjecting them to an electric current. (newscientist.com)
They fuse as one cell," says Wilmut. (newscientist.com)
Wilmut says there were so many failures because it is difficult to ensure that the empty oocyst and the donor cell are at the same stage of the cell division cycle. (newscientist.com)
Wilmut, McWhir, and Campbell collaborated with Angelica Schnieke and Alex J. Kind at PPL Therapeutics in Roslin, a company researching cloning and genetic manipulation for livestock. (asu.edu)

Our understanding of the fate potentials and lineage relationships of these cells is being re-evaluated, both because of recent demonstrations that radial glia can generate neurons and because of the identification of fate-determining genes. (ox.ac.uk)
In the case of PD, where the progressive and selective loss of dopaminergic neurons within the substantia nigra (SN) leads to dopaminergic denervation of the striatum, one possible solution has been to transplant embryonic nigral cells into the host substantia nigra (SN), in an attempt to rewire nigrostriatal circuit. (hindawi.com)
In this article, we first provide a short history of cell therapy in Parkinson's disease and briefly describe the current state-of-art regarding human stem cell-derived dopamine neurons for use in any patient trial. (lu.se)
Several academic and industry efforts are well under owned subsidiary Cyto Therapeutics, it had received way to produce dopaminergic neurons from stem approval by the Australian government to conduct a cells under conditions compliant with use in patients. (lu.se)
Without this, the patient cells lost in PD could be replaced by grafted community is left trying to interpret complex scien- immature human dopaminergic neurons [3, 5]. (lu.se)

To induce differentiation and maturation of the immortalized cell lines, terminating the HPV-E6/E7 expression through a gene induction system has been believed to be essential. (lu.se)
Here, we report that erythroid cell lines established from human bone marrow using simple expression of HPV-E6/E7 are capable of normal erythroid differentiation, without turning gene expression off. (lu.se)
Upon differentiation, other ELLU clones shift from fetal to adult hemoglobin expression, giving rise to more mature cells. (lu.se)
Our findings propose that it is not necessary to employ gene induction systems to establish immortalized erythroid cell lines sustaining differentiation potential and describe novel cellular characteristics for desired functionally competent clones. (lu.se)
The fact that a lamb was derived from an adult cell confirms that differentiation of that cell did not involve the irreversible modification of genetic material required far development to term. (todayinsci.com)
SOX9 has a role in sex determination and differentiation of Sertoli cells. (thermofisher.com)
Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. (thermofisher.com)
The present invention relates to methods of inducing differentiation of stem cells. (justia.com)
In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
These conditions include growing the cells to high or low density, changing media, introducing or removing cytokines, hormones and growth factors, creating an environment which suits differentiation toward a specific cell type, such as providing a suitable substrate. (justia.com)
However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
Various biological events associated with bone healing on implant surfaces can be investigated separately using appropriate cell cultures, such as cell adhesion, proliferation and differentiation, as well as the production and mineralization of extracellular matrix. (bvsalud.org)

Intra-articular injection of expanded autologous bone marrow mesenchymal cells in moderate and severe knee osteoarthritis is safe: a phase I/II study. (sciendo.com)
Although bone marrow fibrosis is seen in a variety of malignant and non-malignant disease states, the deposition of reticulin and collagen fibrosis in the bone marrow of patients with myelofibrosis is believed to be mediated by the myelofibrosis hematopoietic stem/progenitor cell, contributing to an impaired microenvironment favoring malignant over normal hematopoiesis. (haematologica.org)
G-CSF mobilizesCD34+ hematopoietic stem cells from bone marrow into the blood. (fliphtml5.com)
The findingssupport the hypothesis that G-CSF mobilizes two distinct stem cell populations, one from the bone marrow andthe other from the spleen. (fliphtml5.com)
Our hypothesis about a splenic stem cell contributionor maintained survival compared to bone marrow transplants, to PBSCT also derives from the observation that G-CSF mobilizationsalthough graft versus host disease (GVHD) still occurs [1]. (fliphtml5.com)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

NOVOcan: a molecular link among selected glial cells. (ox.ac.uk)
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. (thermofisher.com)
1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)

Cytogenetics and Cell Genetics. (wikipedia.org)
The real experts to ask about the accurate scientific facts of human embryology are the scientific experts in human embryology who are academically credentialed Ph.D. human embryologists - not the "experts" in cell biology, genetics, doctors, nurses, theologians, lawyers or politicians, secretaries, news journalists, etc. (lifeissues.net)
While they succeeded in obtaining cloned macaques, the numbers are too low to make many conclusions, except that it remains a very inefficient and hazardous procedure,' said Robin Lovell-Badge, an embryologist and head of the Division of Stem Cell Biology and Developmental Genetics at the Francis Crick Institute. (cmaaa.co.za)

The deletion of Cish in effector T cells has been shown to augment TCR signaling and subsequent effector cytokine release, proliferation and survival. (wikipedia.org)
This complex mixture makes serum one of the most important supplements supporting the cells growth and proliferation for in vitro cell culture. (diagnovum.com)
Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. (thermofisher.com)
Splenomegaly might reflect dramatic G-CSF-inducedG-CSF stimulation also in multiple studies shows faster recovery of Hox11+ stem cell proliferation. (fliphtml5.com)
Загальні відомості про плазмоклітинні захворювання Plasma cell disorders are a diverse group of disorders of unknown etiology characterized by Disproportionate proliferation of a single clone of B cells Presence of a structurally and electrophoretically. (msdmanuals.com)
Mesenchymal cells were isolated from periodontal ligament of impacted third molars (n=2) and cultured on titanium disks (polished and nitrided) and on a plastic surface as a positive control of cell proliferation. (bvsalud.org)
Cell proliferation was analyzed and growth curves were constructed for the different groups by determining the number of cells adhered to the different surfaces at 24, 48 and 72 h after plating. (bvsalud.org)
We concluded that plasma nitriding produced surfaces that permitted the proliferation of human periodontal ligament mesenchymal cells. (bvsalud.org)
In this respect, this study evaluated the proliferation of mesenchymal cells derived from the periodontal ligament of human third molars on polished and plasma-nitrided titanium surfaces. (bvsalud.org)

Somatic cell cloning (cloning or nuclear transfer) is a technique in which the nucleus (DNA) of a somatic cell is transferred into an enucleated metaphase-II oocyte for the generation of a new individual, genetically identical to the somatic cell donor (Figure 1 ). (biomedcentral.com)

Nor do only the cells of the inner cell mass of the blastocyst become the later adult and none of the cells from the inner cell mass become part of the placenta, umbilical cord, etc. (lifeissues.net)

It did not react with fetal melanocytes, neuroblastoma LA-N-1 cells or a variety of carcinoma, lymphoid, and fibroblastoid cell lines. (bdbiosciences.com)
Moreover, it reacts positively against junctional nevus cells but not intradermal nevi, and against fetal melanocytes but not normal adult melanocytes. (scytek.com)

Presence of Fetal DNA in Maternal Plasma and Serum' (1997), by Dennis Lo, et al. (asu.edu)

A)BACs carry DNA fragments much larger than plasmids or phages and greatly minimize the number of clones needed to make up the genomic library. (easynotecards.com)

1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

The nucleus of a body cell from the DNA donor is removed, and put into the place formerly occupied by the egg's nucleus. (cbc-network.org)
In this process, researchers remove the genetic material from an egg and replace it with the nucleus of some other body cell. (wptv.com)
When the nucleus of a stem cell has been the technique of cloning. (who.int)
The con- is removed and replaced by a nucleus of cept of human cloning has long been in the another cell type, the stem cell will then imagination of many scientists, scholars and be reprogrammed to produce the product fiction writers [ 1 ]. (who.int)
The basic techniques of of the implanted nucleus, when it fully cloning have been known for some time, and develops. (who.int)
The researchers remove the egg's nucleus -- the part of the cell that contains most of its genetic information-- and replace it with the nucleus from another cell. (cmaaa.co.za)
The team tweaked the SCNT procedure using new technology that helped with the nucleus transfer and the fusion of cells. (cmaaa.co.za)

The hybridoma secreting the 9.2.27 antibody was generated using a human melanoma cell extract as the immunogen. (bdbiosciences.com)
Low endotoxin FBS is suitable for sensitive cells, primary cell culture, and hybridoma cloning. (diagnovum.com)
This makes it especially suitable for sensitive cells, primary cell culture, and hybridoma cloning. (diagnovum.com)
To expand diagnostic capacity to detect CVV infections by immunoassays, 12 hybridoma clones secreting anti-CVV murine monoclonal antibodies (MAbs) were developed. (cdc.gov)

Weinzweig , a plastic surgeon who specialized in cleft palate repair, and his team developed a method to cause cleft palates in fetal goats that are similar to clefts that occur in human fetuses. (asu.edu)
Developmental defects, including abnormalities in cloned fetuses and placentas, in addition to high rates of pregnancy loss and neonatal death have been encountered by every research team studying somatic cloning. (biomedcentral.com)
However, regardless of how strongly you support life, you may unknowingly be cooperating in aborted fetal cell research by purchasing products that use aborted fetuses either in the product itself or in its development. (hli.org)

your supposed cloning ban actually authorizes human cloning, implantation, and gestation through the ninth month. (cbc-network.org)
That is what New Jersey legislators did when they passed and then Governor James McGreevey signed S-1909 last year, a law that was sold to the public as outlawing human cloning but which actually permits the creation of cloned human life, and its implantation and gestation up to and including the very moment prior to the emergence of the cloned baby from the birth canal. (cbc-network.org)
The blastocyst can then be transferred to a recipient (h) and cloned animals are born after completion of gestation (i). (biomedcentral.com)

The birth of lambs from differentiated fetal and adult cells also reinforces previous speculation that by inducing donor cells to became quiescent it will be possible to obtain normal development from a wide variety of differentiated cells. (todayinsci.com)
In 1995, they produced a pair of lambs called Megan and Morag from embryonic cells. (shawprize.org)

But they are also less equipped to produce every cell type of the body and less able to reproduce themselves indefinitely, which makes them less appealing to scientists interested in basic research. (eppc.org)
In July 2005, for example, scientists announced that they had engineered adult mouse stem cells into usable mouse eggs, a technique that might one day allow for the creation of human eggs from ordinary human cells. (eppc.org)
Scientists in 1999 created Tetra, a rhesus monkey, but used what researchers consider a simpler cloning method that produces a more limited number of off spring. (cmaaa.co.za)
Because the early stem cells have the ability to become any one of the hundreds of different kinds of human cells, scientists are working on research using these cells with the aim of creating therapies to treat a variety of diseases. (jewishvaluesonline.org)

The primary cloning technique is called "somatic cell nuclear transfer" (SCNT). (cbc-network.org)
If the authors of this bill really meant what they appear to have written, their legislation would ban all human cloning, since as we have seen, biologically, a new human organism, that is, a new human being, comes into existence with the completion of SCNT. (cbc-network.org)
This is junk biology since implanting isn't the act of asexual reproduction: SCNT cloning is. (cbc-network.org)
체세포 핵 치환 (Somatic-cell nuclear transfer, SCNT)은 난자 의 핵 을 제거한 후에, 체세포 의 핵을 이식하여 복제 를 하는 기술을 말한다. (wikipedia.org)
The SCNT technique has worked to create about 20 different animals including frogs, mice, rabbits, pigs, cows and even dogs, but there have been 'numerous attempts to clone non-human primate species, but they all failed,' said Mumming Poo, an author on the paper. (cmaaa.co.za)

For each clone, the Roslin researchers combine material from two sources. (newscientist.com)

These developmental defects have been attributed to incomplete reprogramming of the somatic nuclei by the cloning process. (biomedcentral.com)

The advent of techniques to propagate animals by nuclear transfer, also known as cloning, potentially offers many important applications to animal agriculture, including reproducing highly desired elite sires and dams. (nationalacademies.org)
Because cattle are a species widely used for nuclear transfer studies, and more laboratories have succeeded in cloning cattle than any other specie, this review will be focused on somatic cell cloning of cattle. (biomedcentral.com)
Various strategies have been employed to modify donor cells and the nuclear transfer procedure in attempts to improve the efficiency of nuclear transfer. (biomedcentral.com)

This time, we thought we'd take them from a fetus and an adult as well. (newscientist.com)

Half a century ago, it was found by John Gurdon that this developmental clock can be reversed, and that differentiated somatic cells in a frog model could regain their pluripotency or totipotency. (shawprize.org)

First, they extract immature, unfertilised egg cells called oocytes from the ovaries of ewes. (newscientist.com)
The stem cells of the spleen allow for full maturation of immature transitional B cells into naive B cells. (fliphtml5.com)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)

The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)

The expression of this gene can be induced by IL-2, IL-3, GM-CSF and EPO in hematopoietic cells. (wikipedia.org)
The ectopic expression of human papilloma virus (HPV) E6/E7 gene has successfully been employed to establish these cell lines. (lu.se)
Both cell types shared similar genome-wide gene expression and DNA methylation profiles. (nih.gov)
Similar to iPSCs NT-ESCs displayed clone- and gene-specific aberrations in DNA methylation and allele-specific expression of imprinted genes, similarly to iPSCs. (nih.gov)

Next, the researchers take cells containing donor genetic material. (newscientist.com)
Cells are collected from donor (a) and cultured in vitro (b). (biomedcentral.com)
A matured oocyte (c) is then enucleated (d) and a donor cell is transferred into the enucleated oocyte (e). (biomedcentral.com)
Most of these efforts are focused on donor cells. (biomedcentral.com)

Six parasites ont montré une résistance in vitro au stibogluconate de sodium en utilisant le test de détection des amastigotes dans les macrophages J774 murins. (who.int)
In vitro models have been proposed in an attempt to better understand the early stages of osseointegration and how cells interact with surfaces modified by different methods. (bvsalud.org)

Paul Wagle, M.A., discusses his experience with a life-saving adult stem cell treatment, and the importance of promoting ethical approaches to medical research. (flfamily.org)
The birth of these clones also brings up ethical issues. (cmaaa.co.za)

When they in turn duplicate their genetic material, each cell at the four-cell stage is genetically identical. (wptv.com)
Even while clones are genetically identical, their phenotypes - the characteristics they express - will be different. (wptv.com)

The adoptive transfer of tumor-specific effector T cells knocked out or knocked down for CISH resulted in a significant increase in functional avidity and long-term tumor immunity. (wikipedia.org)
In human tumor-infiltrating lymphocytes (TIL), CISH expression has been reported to be inversely expressed with known T cell activation/exhaustion markers and regulates their expression and neoantigen reactivity. (wikipedia.org)
Tumor cells display a variety of antigens which have been defined by antibodies. (bdbiosciences.com)
We discuss the rationale of various anti-fibrogenic treatment strategies targeting the clonal hematopoietic stem/progenitor cell, aberrant signaling pathways, fibrogenic cytokines, and the tumor microenvironment. (haematologica.org)
The intent of vaccination is to induce a combined antibody and T-cell anti-HER-2 immune response, which is intended to target HER-2-expressing tumor cells, and may induce tumor regression or slow progression of disease. (stanford.edu)
The primary purpose of this study is to determine whether breast cancer tumors respond (as measured by pathologic complete response: the absence of microscopic evidence of invasive tumor cells in the breast) to combined chemotherapy of AC(doxorubicin and cyclophosphamide) followed by paclitaxel plus trastuzumab or lapatinib or both given before surgery to patients with HER2-positive breast cancer. (stanford.edu)
RATIONALE: Drugs used in chemotherapy use different ways to stop tumor cells from dividing so they stop growing or die. (stanford.edu)

Clone 9.2.27 has been shown to react with human melanoma cells, glioma cells, and proliferating brain endothelial cells. (bdbiosciences.com)
We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)
Fetal haemoglobin (HbF) induction is the primary treatment response, along with improved anaemia, reduced haemolysis, myelosuppression and decreased endothelial inflammation. (cdc.gov)

T-cells play a crucial role in the immune response to pathogens by mediating antibody formation and clearance of infected cells, and by defining an overall response strategy. (elifesciences.org)
Human M21 cells were stained with either PE Mouse Anti-Human Chondroitin Sulfate antibody (Cat. (bdbiosciences.com)
The immunogen used to generate the G8.8 antibody was the TE-71 thymic epithelial cell line. (thermofisher.com)
Applications Tested: This G8.8 antibody has been tested by flow cytometric analysis of the TE-71 cell line. (thermofisher.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)
This engineered human IgM constitutively expressed in a HEK-293 stable cell line can replace human-positive control sera in diagnostic serological techniques such as IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA). (cdc.gov)
Compared to the parent murine MAbs, the human-chimeric IgM antibody had identical serological activity to CSGVs in ELISA and demonstrated equivalent reactivity compared to human immune sera in the MAC-ELISA.IMPORTANCEOrthobunyaviruses in the California serogroup cause severe neurological disease in children and adults. (cdc.gov)

3 Myelofibrosis (MF) refers to the Philadelphia chromosome ( BCR-ABL1 )-negative myeloproliferative neoplasm (MPN) originating at the level of the multipotent hematopoietic stem cell. (haematologica.org)

Immunoselected STRO-3+ mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis. (sciendo.com)

During normal development, SOX9 allows the prostate epithelium to outgrow into the mesenchyme and then provides basal cell support for development and maintenance of the luminal epithelium. (thermofisher.com)
Furthermore, CD2 appears very early on fetal thymocytes during development. (mssm.edu)
Laboratory work has centered on the mechanisms of how cells acquire specific fates during growth and development, providing insights into normal and abnormal mechanisms of growth control. (stanford.edu)
NOVOcan was expressed in cells lining the ventricles, including a variety of radial glia during early postnatal development. (ox.ac.uk)
These studies set the stage for determining the roles of NOVOcan in brain cell lineage patterns as well as in other aspects of development. (ox.ac.uk)
The current protein-protein interplay community evaluation recognized these mechanisms concerned within the adaptive and innate immunity, the metabolism of the glucose, most cancers cell development, apoptosis, cell cycle and DNA injury responses. (rbprotein.com)
Food and beverages do not contain any aborted fetal material but may be tastier because of the nature of the research done in their development. (hli.org)
The merge does not seem to have stopped the use of aborted fetal cells in development. (hli.org)
2005). Finally, negative revealed that SSEA4 is detectable in the early neuroepi- selection strategies have been also developed as an alter- thelium, and its expression decreases as development native method to enrich for NSCs from both adult proceeds. (lu.se)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

Recently, the rat and murine homologues have been identified by cDNA cloning. (mssm.edu)

Fetal Bovine Serum (FBS) is the most frequently used growth supplement for cell culture media. (diagnovum.com)
Of special interest is the Fetal Bovine Serum (FBS). (diagnovum.com)
Fetal Bovine Serum Low Endotoxin is a selected FBS featuring an endotoxin level lower than 1 EU/ml. (diagnovum.com)
Description: A sandwich ELISA for quantitative measurement of Human Alpha fetoprotein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (rbprotein.com)

Anatomy43

Organisms17

Diseases9

Chemicals and Drugs84

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes76

Disciplines and Occupations2

Information Science4

Named Groups1

Health Care2

Embryos21

Embryo22

Tissue18

Therapeutic15

Stem-cell res11

Reproductive6

Dolly19

Mammalian7

Tissues4

Mammal1

Differentiate7

Pluripotent7

Sheep4

Mesenchymal stem4

Human embryonic stem2

Germ cells2

Wilmut5

Neurons5

Differentiation19

Bone marrow6

Molecular3

Genetics3

Proliferation9

Transferred into an enucleated1

Placenta1

Melanocytes2

19971

Genomic1

20002

Nucleus7

Hybridoma4

Fetuses3

Gestation3

Lambs2

Scientists4

SCNT5

Roslin1

Nuclei1

Nuclear3

Fetus1

Developmental1

Immature3

Transplantation2

Gene4

Donor4

Vitro2

Ethical2

Genetically identical2

Tumor7

Endothelial4

Antibody7

Hematopoietic1

Precursor cells1

Development10

CDNA1

Serum4