• NBCS is cheaper and better in quality than fetal bovine serum. (coherentmarketinsights.com)
  • As newborn calf serum (NBCS) is more affordable and commonly available, it is preferred as a basal growth medium over fetal bovine serum . (coherentmarketinsights.com)
  • Fetal bovine serum: a pain in the dish? (fcs-free.org)
  • Standard cell culture media commonly consist of a basal medium supplemented with animal serum (such as fetal bovine serum, FBS) as a source of nutrients and other ill-defined factors. (wikipedia.org)
  • Therefore, a chemically defined medium must be entirely free of animal-derived components and cannot contain either fetal bovine serum, bovine serum or human serum. (wikipedia.org)
  • Avoidance of batch to batch variation of bovine serum or albumin, which causes inconsistency in growth-promoting properties. (wikipedia.org)
  • Removal of concerns regarding the limited availability of fetal bovine serum, with periods of world shortages. (wikipedia.org)
  • 2006) despite this product containing bovine serum albumin (Chen et al. (wikipedia.org)
  • Most of these cell culture systems rely on the use of fetal bovine serum (FBS), a supplement of undefined and variable composition. (forschung3r.ch)
  • Aprotinin transcytosis across bovine brain capillary endothelial cell (BBCEC) monolayers is at least 10-fold greater than that of holo-transferrin. (aspetjournals.org)
  • The in vitro model consists of a coculture of bovine brain capillary endothelial cells (BBCEC) and rat glial cells. (aspetjournals.org)
  • Establishment of tumor derived cell lines Principal mouse mammary tumors were minced with a razor blade and digested in DMEM/F12 media sup plemented with 5% fetal bovine serum and 2 mg/ml collagenase for two hours at 37 C. (micrornainhibitors.com)
  • The cell suspensions were separated over Lymphocyte Separation Medium (LSM), density 1.077g/ml (Boehringer). (docksci.com)
  • We developed a calcium signaling-based assay, using cultured human embryonic kidney cells (HEK), that evaluates simultaneously, the activation/desensitization or blockade of the proteinase-activated receptors, PAR 1 and PAR 2 . (aspetjournals.org)
  • The abilities of the PAR 1 -targeted agents to desensitize or block PAR 1 in the HEK cell assay were compared with their activities in a human platelet aggregation assay. (aspetjournals.org)
  • Our data illustrate the usefulness of the HEK cell assay for evaluating the PAR 1 /PAR 2 selectivity of PAR-activating agonists. (aspetjournals.org)
  • To this end, we developed a calcium-signaling assay, employing cultured human embryonic kidney cells (HEK293) in which the action of PAR 1 and PAR 2 agonists and antagonists could be evaluated simultaneously. (aspetjournals.org)
  • Isolated EOC tumor cells obtained at surgery were investigated for their sensitivity to seven standard cytotoxic drugs and nine TKIs using a short‑term fluorescent microculture cytotoxicity assay (FMCA). (spandidos-publications.com)
  • The PSMA affinity of nat Ga-/ nat Lu-PSMA I&T was determined in a competitive binding assay using LNCaP cells. (snmjournals.org)
  • CFU-GM assay CFU-GM were cultured in a medium containing 20% fetal calf serum (FCS), 10% conditioned medium from the human bladder carcinoma cell line 5637 [12] or 10% human placental conditioned medium (HPCM) as a source of CSF [13], 0.3% agar and IMDM in a fully humidified atmosphere at 37°C and 5% CO 2. (docksci.com)
  • Cell migration was determined by Boyden chamber migration assay. (biomedcentral.com)
  • Effects of DA on cell viability and cell volume were studied by using an ATP assay and an electronic cell counter system. (biomedcentral.com)
  • Moreover, an assay using HEp-2 cells as an index of cytotoxicity showed that gas plasma treatment reduced the toxic activity of Stx. (springeropen.com)
  • dCK activity of the cells was determined by an enzyme activity assay. (biomedcentral.com)
  • BL cells and normal fibroblasts were exposed to CDBPA for cytotoxicity screening by XTT assay, followed by cell cycle arrest, determination of apoptosis using Flow Cytometry and by annexin V/FITC/PI assay. (longdom.org)
  • To test the relevance of ADF/cofilin activity for apoptotic processes, we here employed a genetically well-defined system of mouse embryonic fibroblasts (MEFs) devoid of any ADF/cofilin activity and challenged these cells with well-characterized apoptosis inducers. (nature.com)
  • Embryonic stem cells were cultured without feeders in the presence of leukaemia inhibitory factor (LIF) either in medium containing 10% foetal calf serum or in serum-free medium containing the MEK inhibitor PD0325901 (1μM) and the Gsk3 inhibitor CH99021 (3μM), together known as 2i. (nih.gov)
  • This 3D primary cell culture system prepared from embryonic rat brain cells (2-5) contains all types of brain cells (i.e., neurons, astrocytes, oligodendrocytes, and microglia) that are enabled to grow and mature in serum-free medium, and to form highly differentiated structures such as myelinated axons, synapses, and functional neuronal networks. (forschung3r.ch)
  • By utilizing gene expression profiling, we recognized the embryonic stem cell transcrip tion element Nanog like a novel NOTCH1 regulated gene in mammary tumor cells. (micrornainhibitors.com)
  • Previous studies suggest that formation of PCLs can result from transformation of endothelial cells to a proliferative myofibroblast phenotype. (arvojournals.org)
  • RESULTS: Organ culture in TGF-beta1, beta2, or beta3 induced myofibroblast transformation of corneal endothelial cells, with formation of stress fibers containing alpha-SM actin, loss of normal pericellular ZO-1 organization, development of extracellular fibronectin fibrils, and formation of focal contacts as indicated by punctate vinculin staining. (arvojournals.org)
  • V ON WILLEBRAND FACTOR (vWF) is a multimeric glycoprotein (GP) that plays a key role, under high-shear conditions, in the initial attachment of platelets to the extracellular matrix of the endothelial cells after injury of the vessel wall. (ashpublications.org)
  • Endothelin produced by vascular endothelial cells is a potent vasoactive peptide that elicits prolonged contraction of vascular smooth muscle cells. (silverchair.com)
  • MTT (3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide) tests demonstrated that the GA-mPEG 2000 micelle formulation had obvious cytotoxicity to tumor cells and human umbilical vein endothelial cells. (dovepress.com)
  • The BBB is mainly formed by brain capillary endothelial cells that are closely sealed by tight junctions. (aspetjournals.org)
  • Starting with cell source, choice of donor and recipient, as well as isolation methods, we will then discuss existing expansion protocols (two-/three-dimensional cultivation, basal medium, medium supplements, static/dynamic conditions, and hypoxic/normoxic conditions) and influence of these strategies on the cell functionality after implantation. (frontiersin.org)
  • Only a careful consideration and standardization of all pretreatment processes/methods for the different applications of MSCs will ensure robust and reproducible performance of these cell populations in the different experimental and clinical settings. (frontiersin.org)
  • METHODS The experimental samples were obtained from the culture media of conjunctival and corneal epithelial cells, from fractionated blood plasma and leucocytes of healthy subjects and patients with rheumatoid arthritis, and from the tears of healthy subjects and patients with a variety of anterior segment diseases. (bmj.com)
  • Cell culture methods often lead to the reduction of animals used for biological research and industrial applications. (forschung3r.ch)
  • In this work, we investigated the effect of dexamethasone on NO production induced by the epidermal cytokine granulocyte / macrophage colony-stimulating factor (GM-CSF) in a mouse fetal skin dendritic cell line.Methods: NO production was assessed by the method of Griess. (pdffox.com)
  • In most cases, clinically relevant cell numbers for MSC-based therapies can be only obtained by in vitro expansion of isolated cells. (frontiersin.org)
  • We examined the effects of statin drugs on in vitro proliferation, migration and invasion of melanoma cells. (biomedcentral.com)
  • On 1st June 2018, the 3Rs-Centre Utrecht Life Sciences held the symposium ' Improving Science, Advancing Animal Welfare: Towards better In Vitro Practices ', where leading experts outlined the latest developments on good in vitro method practices and discussed the moral and scientific issues related to the use of fetal calf serum (FCS, or FBS). (fcs-free.org)
  • A chemically defined medium is a growth medium suitable for the in vitro cell culture of human or animal cells in which all of the chemical components are known. (wikipedia.org)
  • Therefore, the amount of cholesterol available in vitro may be insufficient, particularly during cell proliferation, repair and maintenance of organ-specific functions (e.g. in cells of the nervous system during neurite extension, synaptogenesis, and myelination). (forschung3r.ch)
  • Uptake of 68 Ga-/ 177 Lu-PSMA I&T in LNCaP tumor cells is highly efficient and PSMA-specific, as demonstrated by competition studies both in vitro and in vivo. (snmjournals.org)
  • Beside bone m a r r o w morphology and k a r y o t y p e [4, 5, 6], bone m a r r o w cultures have attracted great interest [7-10], since the disturbed haematopoiesis in M D S patients is reflected in their in vitro bone m a r r o w growth patterns. (docksci.com)
  • Human granulosa cells (GCs) derived from women undergoing in vitro fertilization (IVF) are an adequate model for endocrine cells of the follicle and the corpus luteum and were therefore employed in an attempt to decipher their DA receptor repertoire and functionality. (biomedcentral.com)
  • In vitro , conditioned medium from iPS-MSCs (iPSMSC-CM) significantly limited albumin-induced tubular apoptosis and enhanced tubular proliferation, accompanied by a reduced expression of tubular Bax and an elevated expression of Bcl2 and survivin. (oncotarget.com)
  • In vitro validation of bosutinib in OSCC cell lines demonstrated that indeed, these cell lines were sensitive to bosutinib with IC 50 of 0.8-1.2 μM. (biomedcentral.com)
  • In vitro, the affinity and internalization kinetics of 111 In-R1507 were determined using the IGF-1R-expressing triple-negative breast cancer cell line SUM149. (snmjournals.org)
  • CDBPA, possesses strong in vitro and in vivo activity against Ramos cells, representing a potential approach for therapy of Burkitt lymphoma. (longdom.org)
  • DTHHS) has developed and coordinated a mixtures program that includes trend analysis to identify the mixtures most often found in environmental media, in vivo and in vitro toxicological testing of mixtures, quantitative modeling of joint action, and methodological development for assessment of joint toxicity. (cdc.gov)
  • Chemically defined culture and differentiation of human pluripotent stem cells: where are we at? (fcs-free.org)
  • Prof. Dr. Paul Honegger and his group at the University of Lausanne developed aggregating cultures of mechanically dissociated primary fetal brain cells which provide an excellent system for neurobiological studies of cellular growth and differentiation. (forschung3r.ch)
  • Defined and animal-component free cell culture medium for differentiation of skeletal muscle cells. (promocell.com)
  • Collectively, the results suggested that nanosilica exerts no effect on RANKL-induced osteoclast differentiation of RAW264.7 cells, although a detailed mechanistic examination of the nSP70-mediated cytotoxic effect is needed. (springer.com)
  • myMATRIX MSC by denovoMATRIX, a German company that develops and manufactures biomimetic coatings for cultivating human stem cells, enhances the expansion process for human mesenchymal stem cells (hMSC) by promoting adhesion, proliferation, and high viability of hMSC (5). (biopharminternational.com)
  • The ability of lovastatin, mevastatin and simvastatin to inhibit the melanoma cell proliferation was examined using cytotoxicity and apoptosis assays. (biomedcentral.com)
  • The concentrations required to inhibit proliferation of melanoma cells (0.8-2.1 μM) have previously been achieved in a phase I clinical trial of lovastatin in patients with solid tumours, (45 mg/kg/day resulted in peak plasma concentrations of approximately 3.9 μM). (biomedcentral.com)
  • New drugs target some of the stepwise genetic mutations the neoplastic cells gain to become masters of their growth and proliferation ( 9 ). (spandidos-publications.com)
  • Our previous studies have shown that the free fatty acid extract (FFAE) of krill oil inhibits the proliferation of both CRC and osteosarcoma cells, and induces the apoptosis of CRC cells [ 17 , 18 ]. (biomedcentral.com)
  • Counts of Ki67-positive cells were used as a measure of endothelial proliferation. (arvojournals.org)
  • However, TGF-beta3 did not stimulate endothelial proliferation above that in serum-free control samples. (arvojournals.org)
  • Serum and bFGF each stimulated proliferation significantly, without inducing myofibroblast transformation. (arvojournals.org)
  • Administration of insulin increased colon cancer cell proliferation and polyp formation in Azoxymethane (AOM)-induced cancer model ( 19 , 20 ). (amegroups.org)
  • The activation of PI3K/Akt pathway can increase cell survival, cell growth and proliferation ( 22-24 ). (amegroups.org)
  • Thus, we conclude that ADF/cofilin activity is not generally required for induction or progression of apoptosis in mammalian cells. (nature.com)
  • Interestingly, mitochondrial association of ADF and n-cofilin during apoptosis was preceded by, and dependent on, actin that translocated by a yet unknown mechanism to mitochondria during cell death. (nature.com)
  • These proteases are responsible for the dismantling of cells during apoptosis. (nature.com)
  • Importantly, the mitochondrial association of n-cofilin and ADF had no impact on apoptosis signaling because ablation of either n-cofilin, ADF, or both proteins together did not change cell death progression. (nature.com)
  • In summary, our work demonstrates that in mammalian cells ADF/cofilin activity is not generally required for efficient induction of apoptosis. (nature.com)
  • Our previous results indicated that BAG-1 large (BAG-1L) overexpression significantly increases cell viability and decreases apoptosis by upregulating HSP70 and p-AKT in response to hypoxia/reoxygenation in SY-SH5Y cells. (bvsalud.org)
  • Fevrier M, Dorgham K, Rebollo A (2011) CD4 + T cell depletion in human immunodeficiency virus (HIV) infection: role of apoptosis. (virosin.org)
  • 1 - 4 Multiple mechanisms may be involved in its potent antitumor effect, including induction of apoptosis, cell cycle arrest, telomerase inhibition, antiangiogenesis activity, and an antimetastasis effect. (dovepress.com)
  • We demonstrated that CDBPA significantly reduced cell viability and induced apoptosis via the extrinsic and the intrinsic apoptotic pathways of Burkitts lymphoma cells without causing cell cycle arrest but reduction of the various phases. (longdom.org)
  • We developed Cell-Ess ® , a novel lipid supplement produced under GMP conditions that serves as a serum-replacement, Dr. Elhofy explains. (genengnews.com)
  • We found that we could boost protein biomanufacturing of mAbs by adding the supplement even to a previously optimized serum-free media in a scalable bioreactor run representative of 10,000 L bioreactor runs. (genengnews.com)
  • The company is currently evaluating the serum-free supplement on other aspects of bioprocessing, including single-use bioreactors, protein quality, and cell metabolism. (genengnews.com)
  • Media formulations containing the media supplement B27 (supplied by Invitrogen) are often erroneously referred to as chemically defined media (e.g. (wikipedia.org)
  • Our results revealed that nSP exerted higher cytotoxicity in macrophage cells compared with submicron-sized silica particles. (springer.com)
  • However, tartrate-resistant acid phosphatase (TRAP) activity and the number of osteoclast cells (TRAP-positive multinucleated cells) were not changed by nSP treatment in the presence of receptor activator of nuclear factor κB ligand (RANKL) at doses that did not induce cytotoxicity by silica particles. (springer.com)
  • On the other hand, it favors exaggerated tissue injury in sepsis, hypersensitivity or autoimmunity, by promoting inflammation or cytotoxicity of innocent bystander cells.1 In the skin, NO is produced by iNOS in several cells, namely in keratinocytes, fibroblasts, Langerhans cells and other dendritic cells. (pdffox.com)
  • 111 In-R1507 (concentration required to inhibit binding by 50%, 0.1 nM) was slowly internalized by SUM149 cells. (snmjournals.org)
  • And the cell concentration is adjusted according to different mitogen or antigen(e.g.Using PHA to stimulate cells, the cell concentration can be 1×10 5 cells/well,for reference only). (biofargo.com)
  • 3. The 10 μL of appropriate concentration of antigen or cell stimulant is added to each well. (biofargo.com)
  • A clear concentration-dependent radiosensitising effect of dFdC was observed in all cell lines. (biomedcentral.com)
  • Consequently, several human diseases, including cancer and neurodegenerative disorders, are caused by defects in this highly regulated form of cell death. (nature.com)
  • In this mini review, we will discuss point by point possible pitfalls in the production of human MSCs for cell therapies, without consideration of material-based applications. (frontiersin.org)
  • We aimed to investigate mechanisms underlying drug-induced cholestasis and improve its early detection using human HepaRG cells and a set of 12 cholestatic drugs and six noncholestatic drugs. (aspetjournals.org)
  • Animal protein-free media, containing human serum albumin, human transferrin, but potentially animal-derived insulin and lipids. (wikipedia.org)
  • This study determines the effects of krill oil extract on the migration of human CRC cells, and its potential role in modulating EGFR signalling pathway and the expression of programmed death ligand 1 (PD-L1). (biomedcentral.com)
  • Human CRC cells, DLD-1 and HT-29 were treated with FFAE of KO at 0.03 and 0.12 μL/100 μL for 8 or 24 h. (biomedcentral.com)
  • High levels of dopamine (DA) were described in human ovary and recently evidence for DA receptors in granulosa and luteal cells has been provided, as well. (biomedcentral.com)
  • DA treatment (48 h) of human GCs resulted in slightly, but significantly enlarged, viable cells. (biomedcentral.com)
  • A previous study showed D 2 in human GCs, which are linked to cAMP, and the present study reveals the full spectrum of DA receptors present in these endocrine cells, which also includes D 2 -like receptors, linked to calcium. (biomedcentral.com)
  • Human induced pluripotent stem cell-derived mesenchymal stem cells (iPS-MSCs) are emerging as attractive options for use in cell replacement therapy, but their effect in kidney diseases remains unknown. (oncotarget.com)
  • The PromoCell Skeletal Muscle Cell Growth Medium is a low-serum medium (5% V/V) developed for the expansion of human skeletal muscle cell cultures. (promocell.com)
  • Although all PromoCell media are optimized for use with primary human cells, we have received feedback from customers that this particular medium can also be used for murine, rat, canine, and rabbit skeletal muscle cells. (promocell.com)
  • DetachKit for gentle and effective detachment of adherent primary human cells. (promocell.com)
  • Primary Human Skeletal Muscle Cells isolated from different skeletal muscles from adult single donors. (promocell.com)
  • In the present study, the relation between deoxycytidine kinase (dCK) activity and the radiosensitising effect of gemcitabine was investigated in eight different human tumour cell lines. (biomedcentral.com)
  • Additionally, it predicted bosutinib, a Src/Abl kinase inhibitor, as a sensitive inhibitor for oral squamous cell carcinoma (OSCC) cell lines. (biomedcentral.com)
  • These results suggest that dexamethasone is a potent inhibitor of intracellular events that are involved on NO synthesis, in skin dendritic cells. (pdffox.com)
  • Benefits presented on this report indicate that Rac1 inhi bition just after incubation of cells using a certain inhibitor or transfection with Rac1 distinct siRNA abrogates IR induced phosphorylation of MEK1/2 and ERK1/2, at the same time since the IR induced G2/M checkpoint activation, suggesting Rac1 as the upstream regulator of IR induced ERK1/2 signaling. (micrornainhibitors.com)
  • For instance, in 2020, Bharat Biotech, a biotechnology company, had conducted research for the production of COVID-19 vaccine, in which newborn calf serum was used as one of the key ingredient for growing the vero cells lines. (coherentmarketinsights.com)
  • Development of an animal-component free medium for vero cells culture. (hal.science)
  • This work describes the development of an animal-component free medium (IPT-AFM) that allows an optimal growth of Vero cells, an adherent cell line used for the production of viral vaccines. (hal.science)
  • According to Dr. Elhofy, Essential Pharma's goal was to develop a means to deliver fats and cholesterol in a system that would increase protein production, yet be chemically defined and free of animal-origin components. (genengnews.com)
  • Our customers in the cell and gene therapy market value the workflow efficiency enabled by Gyrolab solutions, and we look forward to further supporting their efforts to develop the breakthrough therapies they are producing," said Dan Calvo, CEO and president, Gyros Protein Technologies, in a press release. (biopharminternational.com)
  • Cygnus Technologies, a Maravai LifeSciences Company, expanded its host cell protein (HCP) analysis services in June 2019 (4). (biopharminternational.com)
  • The spike (S) protein of SARS-CoV-2 mediates viral receptor binding and membrane fusion, both of which are essential for viral infection of host cells. (nature.com)
  • Peptide-free, protein-free, chemically defined media are rarely successfully formulated except for CHO and insect cells. (wikipedia.org)
  • The presence of the trophic hormone hCG (10 IU/ml) in the culture medium for several days did not alter mRNA (semiquantitative RT-PCR) or protein levels (immunocytochemistry/Western blotting) of D 1,2,4,5 DA receptors. (biomedcentral.com)
  • Oxidative stress was markedly attenuated by iPS-MSCs both in AN mice and in protein-overloaded tubular cells. (oncotarget.com)
  • Gastpar R, Gehrmann M, Bausero MA, Asea A, Gross C, Schroeder JA, Multhoff G (2005) Heat shock protein 70 surface-positive tumor exosomes stimulate migratory and cytolytic activity of natural killer cells. (virosin.org)
  • AR directed siRNA (siAR) used in VCaP cells significantly reduced mRNA expression and AR protein abundance. (springeropen.com)
  • The dCK activity of our cell lines varied considerably and differed up to three fold from 5 to 15 pmol/h/mg protein between the tested cell lines. (biomedcentral.com)
  • The clinically observed relative drug resistance of EOC type I, as well as in patients previously treated, is at least partly due to mechanisms in the tumor cells. (spandidos-publications.com)
  • Internalization kinetics of 68 Ga- and 177 Lu-PSMA I&T were investigated using the same cell line, and biodistribution studies were performed in LNCaP tumor-bearing CD-1 nu/nu mice. (snmjournals.org)
  • Homocitrullination occurs in the tumor microenvironment and CD4-mediated immune responses to Hcit epitopes can target stressed tumor cells and provide a potent antitumor response in mouse models. (bmj.com)
  • We also demonstrated that NOTCH1 sig naling is needed for mammary tumor initiating cell action, as NOTCH1 inhibition success in fast mam mary tumor regression and delays and, in some instances, prevents disorder recurrence. (micrornainhibitors.com)
  • We sought to extend the receptor-desensitization paradigm for use with a cultured cell system, in which an intracellular calcium signal rather than a contractile response might be used as an index of receptor activation. (aspetjournals.org)
  • The effects of propofol on endothelin-1-induced intracellular signaling in an aortic smooth muscle cell line, A10 cells, were examined. (silverchair.com)
  • The effect of propofol on the endothelin-1-induced Ca2+ influx into A10 cells was evaluated by measuring intracellular 45Ca2+. (silverchair.com)
  • These results suggest that propofol inhibits endothelin-1-induced intracellular signaling in vascular smooth muscle cells. (silverchair.com)
  • Other partners of the intracellular metabolism of gemcitabine in relation to the cell cycle effects and DNA repair could be more responsible for the radiosensitising effect than dCK activity. (biomedcentral.com)
  • NP-EGTA AM can serve as a photolabile buffer in cells because, once converted to NP-EGTA by intracellular esterases, it will bind Ca 2+ with high affinity until photolyzed with UV light. (thermofisher.com)
  • Using Medium 199 or MEM as a basal medium, a serum-free medium (SFM) referred as IPT-SFM that only enclosed transferrin as a component of animal origin was developed at first. (hal.science)
  • Such a medium should be free of undefined chemicals and mixtures from animal-derived components, and its exact composition should be published in papers and recorded in databases. (forschung3r.ch)
  • In this study we used CRPC cell models VCaP and LNCaP as well as AR-negative PC-3- and non-neoplastic epithelial BPH-1-cells treated with 5, 10 or 25 μmol/L abiraterone hydrolyzed from abiraterone acetate (AA). (springeropen.com)
  • Samples have been spun down and washed five times in PBS sup discover this plemented with 5% FBS then plated onto 10 cm collagen coated plates in DMEM/F12 media supplemented with 2% FBS and penicillin/streptomycin. (micrornainhibitors.com)
  • 1. Culture cells on a glass-bottom dish using DMEM containing 5% fetal calf serum. (dojindo.com)
  • 2. Change the medium to 1 mM dibutyl cAMP/DMEM, and culture the cells for 3-4 days to induce dendrites. (dojindo.com)
  • There is a clear distinction between serum-based media and chemically defined media. (wikipedia.org)
  • In contrast, chemically defined media require that all of the components must be identified and have their exact concentrations known. (wikipedia.org)
  • To achieve this, chemically defined media is commonly supplemented with recombinant versions of albumin and growth factors, usually derived from rice or E. coli, or synthetic chemical such as the polymer polyvinyl alcohol which can reproduce some of the functions of BSA/HSA. (wikipedia.org)
  • Chemically defined media that are designed for the cultivation of cells in suspension additionally contain suitable surfactants such as poloxamers in order to reduce shear stress caused by shaking and stirring. (wikipedia.org)
  • Chemically defined media also allows researchers who are studying in the field of cell physiology (especially extracellular) and or molecule-cell interactions to eliminate any variables that may arise due to the effects of unknown components in the medium. (wikipedia.org)
  • Every expression system-every scheme for optimizing nutrients and cell culture conditions-presents advantages, disadvantages, and tradeoffs with respect to costs, yields, quality metrics, contaminants, availability of system components, time constraints, and so on. (genengnews.com)
  • In the organism, liposoluble nutrients such as cholesterol, fatty acids and lipophilic vitamins reach the cells by means of specific apolipoproteins which form complexes with the lipids, the so-called lipoproteins (LPs), and which recognize specific membrane receptors expressed by the cells. (forschung3r.ch)
  • Statistical experimental design was applied to identify crucial nutrients that affect cell growth. (hal.science)
  • Cell-Ess can deliver fats and cholesterol, boosting the ability of cells to make membranes in either basic research and bioprocessing applications. (genengnews.com)
  • It is an important building block of all cell membranes, and an important metabolite in many biosynthetic pathways. (forschung3r.ch)
  • To determine TRPA1 localization in cellular membranes we stained HEK293T cells transfected with the mTRPA1-mCherry construct with the Vybrant Alexa Fluor 488 Lipid Raft Labeling Kit. (elifesciences.org)
  • Biological immortality Cell culture assays Microphysiometry Organ culture Plant tissue culture Tissue culture Jayme DW, Smith SR (2000). (wikipedia.org)
  • Another is to adapt useful cell lines individually for survival and growth in serum-free culture media. (forschung3r.ch)
  • Drug activity was compared with respect to EOC subtype, preoperative chemotherapy, cross‑resistance and association with progression‑free survival (PFS). (spandidos-publications.com)
  • Revision accepted 3 September 1989) Abstract--Bone marrow cultures and survival time were studied in 39 patients with primary myelodysplastic syndromes. (docksci.com)
  • Survival was calculated from the day of first bone marrow culture (i.e. presentation at the hospital) until death. (docksci.com)
  • Bone marrow cultures and survival time were studied in 39 patients with primary myelodysplastic syndromes. (docksci.com)
  • Cell survival was determined 7, 8, or 9 days after RT by the sulforhodamine B test. (biomedcentral.com)
  • Despite almost 50 years of research and over 20 years of preclinical and clinical studies, the question of curative potential of mesenchymal stem/stromal cells (MSCs) is still widely discussed in the scientific community. (frontiersin.org)
  • Mesenchymal stem/stromal cells (MSCs) have the capacity to differentiate into cells and tissues of one germ layer, here the mesodermal lineage, and are consequently multipotent. (frontiersin.org)
  • This trend, well advanced for basal media, is also progressing for media supplements. (genengnews.com)
  • However, there is a push to utilize supplements designed to eliminate serum completely. (genengnews.com)
  • Unfortunately, serum-free supplements still have significant challenges. (genengnews.com)
  • Ovarian DA can act thus via D 1,2,4,5 , which are co-expressed by endocrine cells of the follicle and the corpus luteum and are linked to different signaling pathways. (biomedcentral.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • The culture dishes were examined under an inverted microscope for colony (/>40 cells) and cluster (3-39 cells) growth after 7 days of incubation, with an additional examination for colonies after 10 days. (docksci.com)
  • Fura 2-AM is an acetoxymethyl ester derivative of Fura 2 that can be easily loaded into cells by incubation. (dojindo.com)
  • The specific incubation time varies due to different cell types and cytokines. (biofargo.com)
  • When confluent, cells have been passaged at a one,2 or one,three dilutions after a five minute incubation with Versene and maintained on standard tissue culture plates. (micrornainhibitors.com)
  • Herein, we present the first evaluation of cationic dendrimers based on 2,2-bis(methylol)propionic acid (bis-MPA) as nonviral vectors for transfection of short interfering RNA (siRNA) in cell cultures. (mdpi.com)
  • 1) One is to replace serum by other biological but as yet inadequately defined mixtures such as extracts from plants, animal tissues or cells. (forschung3r.ch)
  • The detection of harmful stimuli such as noxious chemicals and changes in temperature and pressure are fundamental biological processes carried out by numerous cell types through diverse specialized receptors. (elifesciences.org)
  • This free radical acts as a cytotoxic agent against exogenous microorganisms, by stimulating macrophage-induced inflammation and death of infected cells. (pdffox.com)
  • This study tested insulin-caused resistance to oxaliplatin via activation of PI3K/Akt pathway in HT29 cells. (amegroups.org)
  • Insulin decreased drug efficacy of oxaliplatin in HT29 cells, which could be mediated by the activation of the PI3K/Akt pathway. (amegroups.org)
  • As the activation of PI3K/Akt signal pathway increases the resistance of several cancer cell lines such ovarian, lung cancer to chemotherapeutic drugs ( 7 , 8 ), it is possible that PI3K/Akt may also play a role in the poor prognosis of obesity-associated colon cancer. (amegroups.org)
  • The technical disadvantages to using serum include its undefined nature, batch-to-batch variability in composition, and the risk of contamination. (wikipedia.org)
  • Addition of dexamethasone to the culture, 30 min before GM-CSF stimulation, significantly inhibited the cellular expression of iNOS. (pdffox.com)
  • Few balancing acts are as delicate as the optimization of a cell expression system, especially if the expression system is to yield recombinant proteins destined for therapeutic use. (genengnews.com)
  • Further, there were no significant changes in metabolites or increases in biomass such as cell debris and host proteins. (genengnews.com)
  • This parallel detection of PB1-F2 and NP suggests that applied sensor chip technology may be amenable to an arrow immunosensor for simultaneous detection of all known influenza virus proteins in infected tissues and cells. (omicsonline.org)
  • Serum-based media may contain undefined animal-derived products such as serum (purified from blood), hydrolysates, growth factors, hormones, carrier proteins, and attachment factors. (wikipedia.org)
  • In the present project (No 109-08 ) the utility of blood serum or plasma proteins for FBS replacement was investigated. (forschung3r.ch)
  • Background Post-translational modification of proteins has the potential to alter the ability of T cells to recognize major histocompatibility complex (MHC) class -I and class-II restricted antigens, thereby resulting in altered immune responses. (bmj.com)
  • Post-translational modifications of proteins provide targets for T cells that have not been subject to central tolerance. (bmj.com)
  • Homocitrullination of proteins occurs in tumors and can be targeted by CD4 T-cell responses. (bmj.com)
  • This study provides the first evidence that homocitrullinated proteins in tumors can also be targets for CD8 T-cell responses. (bmj.com)
  • This research suggests that post-translational modification of proteins provide efficient targets in tumors for both CD4 and CD8 T-cell-mediated therapies. (bmj.com)
  • Non-reproducible treatment outcomes or even absence of treatment effects in comparison to control groups challenges the potential of these cells for routine application both in tissue engineering and in regenerative medicine. (frontiersin.org)
  • How to find reproducible culture conditions? (forschung3r.ch)
  • Nonetheless, the outcomes of this report propose that IR induced G2/M cell cycle arrest as well as the regulation selleck chemicals PF299804 of Rac1 on the IR induced G2/M checkpoint response is apparently inde pendent of p53, as among the four breast cancer cell lines applied to the studies, MDA MB 231 and T47D cells express mutant p53, whereas MCF seven and ZR75 1 express wild sort p53. (micrornainhibitors.com)
  • We isolated the following BA.2 variants in VeroE6/TMPRSS2 cells: hCoV-19/Japan/UT-NCD1288-2N/2022 (NCD1288), hCoV-19/Japan/UT-HP353-1N/2022 (HP353), hCoV-19/Japan/UT-HP354-1N/2022 (HP354), and hCoV-19/Japan/TY40-385/2022 (TY40-385). (nature.com)
  • The global newborn calf serum market is estimated to be valued at US$ 222.2 Mn in 2022 and is expected to exhibit a CAGR of 6.8% over the forecast period (2022-2030). (coherentmarketinsights.com)
  • For instance, in June 2022, NorthX Biologics, a biologics company with the help of Alder Therapeutics, a pharmaceutical company expanded its services for cell therapy and established Good Manufacturing Practice (GMP)-manufacturing labs at Matfors facility and Karolinska University Hospital campus in Stockholm. (coherentmarketinsights.com)
  • The problem is that serum contains more than 300 factors and provides beneficial factors such as fats, cholesterol, small molecules, and hormones," he says. (genengnews.com)
  • Unexpectedly it was found, that the beneficial effects of serum do not reside in any of the examined serum lipoprotein fractions (VLDL/IDL, LDL, HDL), but remain in the lipoprotein-deficient macromolecular FBS fraction. (forschung3r.ch)
  • As further confirmation, we demonstrated experimentally that bosutinib has anti-proliferative activity in OSCC cell lines, demonstrating that DeSigN was able to robustly predict drug that could be beneficial for tumour control. (biomedcentral.com)
  • Analysis of heterologous expression systems of rOAT1 in oocytes and culture cells revealed that rOAT1 has the ability to transport anionic drugs, such as β-lactam antibiotics, NSAIDs, methotrexate, and antiviral drugs, as well as various endogenous organic anions and exogenous substances. (aspetjournals.org)
  • When a cell line is tough and cell attachment is a major concern during cell culture, newborn calf serum is often used. (coherentmarketinsights.com)
  • In 2012, the repertoire of cancer cell lines used was expanded substantially with the inclusion of new data from the Genomics of Drug Sensitivity in Cancer (GDSC) and Cancer Cell Line Encyclopedia (CCLE) projects where 707 and 860 cancer cell lines respectively were assembled for anti-cancer drug testing. (biomedcentral.com)
  • The degree of radiosensitisation was also cell line dependent and seemed to correlate with the sensitivity of the cell line to the cytotoxic effect of dFdC. (biomedcentral.com)
  • E14Tg2a, RGD2, Rex-GFP and HM1 cells are male ES cells of 129 background, established and grown in serum without feeders according to standard protocols. (nih.gov)
  • Before clinical trials new drugs are tested on cell lines, these cell lines are grown with the use of serum, as one of the key ingredient in growing medium. (coherentmarketinsights.com)
  • EOC can include cells of origin from both the ovarian surface epithelium and the fallopian tube ( 2 ). (spandidos-publications.com)
  • Thus, type I (low-grade serous G1, low-grade endometrioid G1/G2, mucinous or clear cell) tumors are associated with corresponding benign ovarian cystic neoplasms, often developing through an intermediate borderline step and have a better prognosis. (spandidos-publications.com)
  • On prostate cancer (PC) cells, the cell surface enzyme prostate-specific membrane antigen (PSMA), also known as glutamate carboxypeptidase II or N -acetyl- l -aspartyl- l -glutamate peptidase, is highly upregulated, whereas it shows low or no expression in the normal prostate ( 3 ). (snmjournals.org)
  • NorthX is doing this expansion due to increasing needs of patients and growing cell therapies market. (coherentmarketinsights.com)
  • Coating tissue-culture flasks with teleostean, a product extracted from cold water fish skin, had not only enhanced cell attachment but also improved cell growth performance in static cultures. (hal.science)
  • In one case, lipids are solubilized with alcohol, but this causes oxidation and destabilization of free fatty acids. (genengnews.com)
  • Our previous studies have shown that the free fatty acid extract (FFAE) of krill oil exhibits anti-proliferative and pro-apoptotic properties. (biomedcentral.com)
  • For example, with animal cell cultures, the trend is away from serum and toward defined media, that is, media uncomplicated by the presence of animal-origin components. (genengnews.com)
  • To facilitate cell culture optimization, Essential Pharma has developed Cell-Ess, a serum replacement that is free of animal-origin components. (genengnews.com)
  • The origin of CYP17A1 up-regulation under AA treatment was investigated in CRPC cell models by qRT-PCR and western-blot procedures. (springeropen.com)
  • Furthermore, we reasoned that, provided receptor cross-desensitization did not occur, it would be advantageous to assess PAR-targeted ligands in a cell that expressed both receptors. (aspetjournals.org)
  • TrypLE Select, a recombinant trypsin, gave the best cell growth performances. (hal.science)
  • With both receptors present in the same cell, the selectivity or nonselectivity of a variety of compounds that would affect PAR 1 and/or PAR 2 could be efficiently evaluated in a single experiment. (aspetjournals.org)