Fertilization calcium signaling. Medical search. Frequent questions

The molecular components of key Ca2+ signalling pathways have been identified. (hstalks.com)
Consequently our understanding of Ca2+ signalling is now advancing rapidly through experimental approaches that involve the controlled expression, inhibition of expression and use of naturally occurring or engineered mutations of channels, pumps, effectors and other proteins related to Ca2+ signalling pathways. (hstalks.com)
Both the inositol 1,4,5-trisphosphate (InsP(3)) and ryanodine receptor pathways contribute to the Ca(2+) transient at fertilization in sea urchin eggs. (ox.ac.uk)
Activated TPKR could activate its downstream signaling elements, like Ras/Raf/MEK/ERK, PI3K/Akt/eIF4E, PI3K/Akt/eNOS and PLC-gamma/PKC pathways. (biomedcentral.com)
With this strategy, the roles of additional signaling pathways in sperm capacitation and fertilization can be resolved. (bvsalud.org)
Our goal is to better define these signaling pathways at the cellular and molecular levels to identify potential therapeutic targets in various disease states. (hamad.qa)
the case of calcium (Ca2+), they play a critical role in modulating intracellular signaling pathways. (researchhunt.com)
The discovery of calcium-mediated signalling pathways in the regulation of cellular function has truly revolutionized the fields of life science and medicine. (shawprize.org)
These discoveries represent one of the most important cell signalling pathways in biology and have changed forever the way we think about prevention and treatment of disease. (shawprize.org)
Cellular communication occurs through chemical signals such as hormones, neurotransmitters and nitric oxide, which act via specific receptors or receptive molecules and are linked to diverse intracellular and extracellular signalling pathways. (shawprize.org)
Signal transduction pathways mediated by the changes in the [Ca2+]c is termed Ca2+ signaling, and are mainly initiated by the activation of Ca2+-permeable channels. (globalsciencebooks.info)
Biphasic role of calcium in mouse sperm capacitation signaling pathways. (umassmed.edu)
Cdc42 localized in the CatSper signaling complex regulates cAMP-dependent pathways in mouse sperm. (umassmed.edu)

Following fertilization, a signaling pathway induces the cortical granules to fuse with the oocyte's cell membrane and release their contents into the oocyte's extracellular matrix. (wikipedia.org)
read more keep the cytoplasmic calcium ([Ca2+]c) low and then allow selective increases in [Ca2+]c by stimulating either Ca2+ release from the cells' internal Ca2+ stores or the influx of Ca2+ from the extracellular environment. (hstalks.com)
In the present study, we screened de novo assembled RNAseq from ovary, testis, and oviduct samples in C. pyrrhogaster and identified the base sequences encoding zona pellucida (ZP) proteins, voltage-dependent Ca 2+ channels, and cysteine-rich secretory proteins (CRISPs), which respectively are sperm receptors for egg envelopes, major mediators of sperm intracellular signaling, and expected extracellular modulators for sperm function in the female reproductive tract. (ehu.eus)
Recently, a large number of signaling proteins reported play an important key role in the process of LPLI-induced cell proliferation, probably due to the fact that the molecular events they are involved in are the basic response of the cells to extracellular stimuli. (biomedcentral.com)
The calcium waves occurred in the absence of extracellular calcium and were inhibited in embryos injected with heparin, an inositol 1,4,5-trisphosphate (InsP 3 ) receptor antagonist. (elsevierpure.com)

As a critical component of the signaling pathway, the RyR medicated calcium-induced calcium release signaling system, has been well studied along with their regulator FK506-binding protein 12 (FKBP12/Calstabin). (biomedcentral.com)
The NO pathway acts late during the fertilization response in sea urchin eggs. (ox.ac.uk)
In the present study we pursue the hypothesis that NO is a primary egg activator in sea urchin eggs and build on previous findings that an NO/cGMP/cyclic ADP-ribose (cADPR) pathway is active at fertilization in sea urchin eggs to define its role. (ox.ac.uk)
Cyclic ADP-ribose, the ADP-ribosyl cyclase pathway and calcium signalling. (ox.ac.uk)
This review will focus on the known molecules that regulate myoblast fusion with an emphasis on a novel signaling pathway involving the calcium-regulated transcription factor NFATC2 in the regulation of myoblast fusion. (horsleylab.org)

As a core metal ion in signaling transduction, calcium content is maintained in dynamic balance under normal circumstances. (mdpi.com)

These results suggest that InsP 3 receptor mediated calcium mobilization plays an essential role in calcium wave formation at the cleavage furrows. (elsevierpure.com)
PROKR2 missense mutations associated with Kallmann syndrome impair receptor signalling-activity. (medscape.com)

In zebrafish sperm, a more alkaline pH inside the cell causes calcium ions to flow in and this influx of calcium ions triggers a unique spinning-like swimming movement that is different from the swimming of other sperm from other species. (elifesciences.org)

With the discovery that calcium signals are the primary trigger for oocyte activation, a key remaining question became the identification of the signaling protein that mediates such calcium signals at fertilization. (umass.edu)
Many protein components of this signaling cascade have been cloned and characterized: Strikingly, many exist as different isoforms, the number of which is further increased through mechanisms of alternative splicing of the primary transcripts. (schoolbag.info)
This fusion protein was specifically designed to enable the concurrent observation of acrosomal calcium dynamics and membrane fusion events. (bvsalud.org)
When Ca2+ channels are activated in response to a variety stimuli, a drastic increase in the [Ca2+]c is induced and the entered free Ca2+ binds to the sets of Ca2+-regulated proteins such as calmodulin and calcium dependent protein kinases to modify the activities or affinities of these proteins in binding to specific targets. (globalsciencebooks.info)
Research into a genetic mutation causing some men to be infertile shows that an important protein in the sperm that is a key component of the egg fertilization process, known as phospholipase C zeta (PLC-zeta), is ineffective in these individuals. (biotexcom.com)
The investigators found that they could restart the fertilization process in the lab by injecting eggs with a higher amount of the PLC-zeta protein from the infertile men than found naturally in their sperm, which suggests that this type of infertility could become reversible with medical assistance. (biotexcom.com)
Recent clinical studies have highlighted the crucial importance of PLC-zeta in human fertilization, reporting that sperm from infertile patients, who exhibited failed fertilization even after the most powerful in vitro fertilization techniques, contained either reduced amounts or mutated forms of this protein," said Dr Michail Nomikos (Qatar University, Doha, Qatar) lead author of the Biochemical Journal paper. (biotexcom.com)
They found that injecting the abnormal PLC-zeta protein into mouse eggs at levels comparable with those found in the sperm of the infertile men resulted in no calcium oscillations and therefore no fertilization. (biotexcom.com)
However, if they increased the amounts of the protein they injected to a higher level than would be present naturally, the normal fertilization process began and calcium oscillations were observed. (biotexcom.com)
Identification of such an egg protein will be a major breakthrough in the fertilization field as it potentially may lead to identification of currently unknown causes of female infertility. (biotexcom.com)
For example, in Timothy Syndrome the mutation in the gene produces too much of the protein, in this case the L-type calcium channel Cav1.2. (epiphanyasd.com)

Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models. (umassmed.edu)
Phospholipase C-zeta deficiency as a cause for repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization with ICSI: a case report. (umassmed.edu)
Assisted reproductive technologies, including in vitro fertilization (IVF), zygote intrafallopian transfer (ZIFT), and gamete intrafallopian transfer (GIFT), have been used successfully when male patients with Kallmann syndrome or idiopathic hypogonadotropic hypogonadism do not achieve adequate sperm counts on either GnRH or gonadotropin therapy. (medscape.com)
Taking DHEA by mouth before in-vitro fertilization (IVF) might improve the chances of pregnancy and having a baby. (medlineplus.gov)

This is because the small peptide molecule serves as a signal to the female part of the flower, like a knock on the door letting the female part know the pollen tube is here. (nob6.com)

Molecular mechanisms regulating the meiotic maturation and fertilization of starfish and sea urchin eggs. (szn.it)
Secondly, I am studying the role of the cortical and nuclear actin in the modulation of the calcium signalling during the process of maturation and fertilization of starfish and sea urchin eggs. (szn.it)
This effect is mirrored by the inhibition of cGMP and cADPR signaling in sea urchin eggs at fertilization. (ox.ac.uk)
Recent data suggests that cyclic adenosine diphosphate-ribose may function as a second messenger in sea urchin eggs at fertilization and in stimulus secretion coupling in pancreatic beta-cells. (ox.ac.uk)

Thus, we can now propose that intricately choreographed calcium signaling through gap junctions plays a pivotal role in steering the early development of the embryo. (uib.no)

It will show that Ca 2+ itself is central in the regulation of the Ca 2+ signal. (schoolbag.info)
They also function in a diverse set of mechanisms such as calcium hemostasis, cell signaling, regulation of programmed cell death (apoptosis), and biosynthesis of heme proteins that carry oxygen. (asu.edu)
for his discoveries on calcium signalling in the regulation of cellular activity. (shawprize.org)

Electrical, morphological and biochemical modifications occurring through all the steps of the reproductive process involve ion channels activity, calcium release and oscillations, molecules, metabolites and messengers of different natures. (mdpi.com)
On the contrary, many information are available on marine natural products whose target molecules play a key role in the main steps of gametogenesis, fertilization and embryo development. (mdpi.com)
Lastly, we used RNA knockdown methods to diminish the expression of connexin proteins, which generate so-called "gap junctions" between cells - channels of communication which calcium and other molecules can traverse. (uib.no)
This channel is activated by signalling molecules inside cells, called 'cyclic nucleotides', and its activity ultimately leads to calcium ions flowing into the sperm cell's tail. (elifesciences.org)
Lipid signaling employs various messenger molecules that carry out communication between the plasma membrane, the endomembrane compartments, and the cytoplasm. (frontiersin.org)

In sperm of marine invertebrates and mammals, Ca 2+ signalling has been intensely studied, whereas for fish little is known. (elifesciences.org)
In mammals, cell fusion occurs among a limited number of cell types: sperm and oocytes during fertilization, trophoblasts during placenta formation, macrophages during giant cell and osteoclast formation and myoblasts in the formation of myofibers and myotubes. (horsleylab.org)

The application of molecular biology to Ca2+ signalling research has also opened new avenues of investigation. (hstalks.com)
An understanding of the molecular basis of this interaction is expected to elucidate the evolutionary history of internal fertilization in amphibians. (ehu.eus)
Recently, deep sequencing technology has provided global gene information even in nonmodel animals, allowing us to understand specific features of the molecular mechanisms underlying fertilization in C. pyrrhogaster . (ehu.eus)
Calcium signaling are conserved from invertebrates to vertebrates and plays critical roles in many molecular mechanisms of embryogenesis and postnatal development. (biomedcentral.com)
Understanding the intricate molecular processes of fertilisation may help improve the commercial yields in flowering plants, according to Luan. (nob6.com)
This Research Topic aimed to collect, collate, and summarize the most recent and cutting-edge advances and propositions studying the molecular determinants and effectors of calcium signalling and homeostasis throughout the early events of fertilisation and preimplantation embryogenesis, with a specific view to examine how calcium can affect the efficacy of such important phenomena. (edu.qa)
The knowledge gained on how various factors increase calcium mobilization and how calcium controls cellular activity has widely expanded the areas of cell and molecular biology, and has led to the development of novel therapeutic strategies ranging from the treatment of heart disease to the improvement of learning and memory. (shawprize.org)

Calcium plays an important role in this communication, because it exerts regulatory effects on many cellular proteins and cellular processes. (uib.no)
The unique combination of ZP proteins suggests a new mechanism for sperm binding to egg envelopes in the internal fertilization of C. pyrrhogaster . (ehu.eus)
Recently, the signaling proteins involved in LPLI-induced proliferation merit special attention, some of which are regulated by mitochondrial signaling. (biomedcentral.com)
The reason for this redundancy is not yet completely understood, but emerging evidence suggests that each variant of the Ca 2+ -controlling proteins has a precise role in the shaping of the Ca 2+ signal. (schoolbag.info)

Ca2+ signals can be complex with some involving periodic oscillations of varying frequencies or responses that are restricted to specific regions of the cell. (hstalks.com)
Even more, in the case of oscillations, messages can be read differently according to the frequency of the oscillatory signals. (schoolbag.info)
It has been known for some time that an essential part of the human fertilization process is a repetitive, dramatic increase in calcium levels in the egg known as 'calcium oscillations' triggered by a message from the sperm that happens just before an embryo begins to form. (biotexcom.com)

The sperm-specific ion channel CatSper is the principal calcium channel in sperm mediating the calcium influx into the sperm flagellum and acting as an essential modulator of downstream mechanisms involved in fertilization. (123dok.net)
Considering its pleiotropic role, it seems evident that the cells, to get a specific message in the proper time and manner, need precise and efficient mechanisms to encode and decode Ca 2+ signals. (schoolbag.info)
The mechanisms by which cells decode Ca 2+ signals are now explored in numerous laboratories. (schoolbag.info)

In the last two decades, improvements in the development of probes and instrumentation for Ca 2+ imaging have led to the discovery that the coordinated action of different players is responsible for a complex spatio-temporal organization of the Ca 2+ signal. (schoolbag.info)
however, the detailed spatio-temporal pattern of calcium dynamics has remained unclear. (elsevierpure.com)
Phosphatidic acid (PA) is an important intermediate in membrane lipid metabolism that acts as a key component of signaling networks, regulating the spatio-temporal dynamics of the endomembrane system and the cytoskeleton. (frontiersin.org)

Movement of calcium ions within sperm cells, through calcium ion channels, plays a major role on sperm cell function, according to Skakkebaek. (newswise.com)
In the testis, CaV1.1, 1.2, and 3.2, which are L- and T-type voltage-dependent Ca 2+ channels, were found as potential mediators for the internal fertilization-specific sperm-egg interaction. (ehu.eus)
According to the paper, the calcium channels identified in the study are unique to plants. (nob6.com)
According to Luan, researchers have studied calcium channels for more than 30 years, uncovering how they confer resistance to powdery mildew, a fungal disease that affects a wide variety of plants. (nob6.com)
The biochemical role of calcium channels remained unknown until this study uncovered the specific channel activity. (nob6.com)
Chapter 3 Biochemistry and cell biology of calcium channels and signaling involved in plant growth and environmental responses. (globalsciencebooks.info)
In the plants being exposed to environmental stresses, ion channels are likely activated to convert these external stimuli into intracellular signals. (globalsciencebooks.info)
So we return to the subject of calcium channels. (epiphanyasd.com)
The current drugs used to block calcium channels were mainly developed to treat heart conditions. (epiphanyasd.com)
You can both turn on self-injury via activating L type calcium channels and extinguish it by blocking the same channels. (epiphanyasd.com)

This influx of calcium ions in turn controls the beating of the tail and, thereby, steers the sperm cell towards the egg. (elifesciences.org)
Binding of progesterone to CatSper causes a temporary influx, or surge, of calcium ions into the sperm cell, controlling several sperm functions necessary for fertilization. (newswise.com)
Furthermore, nine of the 13 UV filters seem to induce this calcium ion influx by directly activating the CatSper channel, thereby mimicking the effect of progesterone. (newswise.com)

CatSper is a sperm-specific calcium ion channel that he said is essential for male fertility. (newswise.com)
C2CD6 regulates targeting and organization of the CatSper calcium channel complex in sperm flagella. (umassmed.edu)

Perhaps no other single ion plays such a profound and determinative role in reproductive success as calcium, since it is critical for germinal vesicle breakdown, sperm capacitation and hyperactivation, oocyte activation, maintenance of zygote calcium homeostasis and embryogenic developmental gradients. (edu.qa)
We are particularly interested in the role of calcium in cancer, cardiovascular and immune disfunction, as well as in oocyte maturation in preparation for fertilization. (hamad.qa)
Each month, highly coordinated hormonal and ovarian morphological changes develop and release a mature oocyte that is ready for fertilization. (medscape.com)

To guarantee the specificity of the signal transmission, the cell organizes dynamically the Ca 2+ fluctuations in the cytosol by varying the distribution, the type, and the availability of the different Ca 2+ transporters, and it increases the spatial and temporal complexity of Ca 2+ homeostasis by compartmentalizing the signals into the organelles. (schoolbag.info)
The control of their Ca 2+ homeostasis is not only essential in the control of organelle-specific processes, but it is also fundamental in the overall dynamic modulation of the Ca 2+ signaling in the cytosol. (schoolbag.info)
Calcium homeostasis exerts a profound role in determining the quality of gametogenesis, the resultant efficacy of fertilisation and ultimately the competency of embryogenesis. (edu.qa)
It is described as a housekeeping enzyme, being responsible for cell-to-cell signaling, tissue homeostasis, and cytoprotection. (medscape.com)

Cortical granules are regulatory secretory organelles (ranging from 0.2 um to 0.6 um in diameter) found within oocytes and are most associated with polyspermy prevention after the event of fertilization. (wikipedia.org)
A crucial prerequisite for fertilization and normal embryonic development is the proper synchronization of the interactions between the cytoplasm and the nucleus of the oocytes during the meiotic maturation. (szn.it)
The availability of a large number of oocytes makes the starfish an excellent experimental model system in which to study biochemical and morphological events associated with maturation, fertilization, and early development. (szn.it)
The major aim of my PhD project is to characterize the intracellular calcium increases in the maturing oocytes and fertilized eggs, and to study their physiological functions. (szn.it)

Cyclic adenosine diphosphate-ribose may have a similar role to inositol trisphosphate in controlling intracellular calcium signalling with these two calcium-mobilizing second messengers activating ryanodine receptors and inositol trisphosphate receptors respectively. (ox.ac.uk)
This signalling system liberates two key second messengers, namely, IP 3 (inositol 1,4,5-trisphosphate) and DAG (diacylglycerol). (shawprize.org)

It regulates life and death processes that include fertilization, cell proliferation, apoptosis and cell necrosis. (hstalks.com)
However, our understanding of how calcium signaling regulates AE is still incomplete. (bvsalud.org)

Phosphoinositides (PPIs) together with various kinases and phosphatases were described as specific lipids and enzymes with signaling functions earlier ( Martin, 1998 ). (frontiersin.org)

In this recently published study , we dynamically imaged changes in intracellular calcium concentration in the early embryos of Oikopleura dioica using the genetically encoded calcium indicator GCaMP6. (uib.no)

The sensor combines a genetically encoded calcium indicator (GCaMP) fused with mCherry. (bvsalud.org)

Calcium ions (Ca2+) are involved in nearly every aspect of cellular life. (123dok.net)
Specifically, the investigators evaluated calcium signaling, which is signaling inside the cell brought on by changes in the concentration of calcium ions. (newswise.com)

James M Cummins published 'The Role of Maternal Mitochondria during Oogenesis, Fertilization and Embryogenesis' 30 January 2002 in Reproductive BioMedicine Online. (asu.edu)

In plants, CDPKs translate Ca 2+ signals generated by external stimuli into cellular responses, thereby regulating cell division and differentiation, the development of tolerance to stress stimuli and the specific defense responses to pathogens. (pberghei.eu)

Once activated, peptide receptors recruit a calcium channel that guides the pollen tube to the ovule and initiates fertilisation, the study said. (nob6.com)
A new life begins when the sperm fuses with the egg to inject an enzyme that initiates pulses of calcium that occur every two minutes for two hours and is responsible for activating egg development. (biotexcom.com)

Starvation induces an increase in intracellular calcium and potentiates the progesterone-induced mouse sperm acrosome reaction. (umassmed.edu)

Michael Berridge , born 1938 is Honorary Professor of Cell Signalling at the University of Cambridge and was Deputy Chief Scientist at the Babraham Institute in Cambridge, UK, until 2003. (shawprize.org)

By contrast, mammalian fertilization happens in confined compartments of the female oviduct. (elifesciences.org)

Calcium (Ca2+) plays a major role in controlling cell function. (hstalks.com)
These finding indicate that DmFKBP12 and RyR associated calcium signaling plays an essential role in the successful development of Drosophila melanogaster . (biomedcentral.com)

This IP 3 /Ca 2+ signalling system is of fundamental importance in regulating diverse cellular processes such as muscle contraction, cell growth and differentiation, secretion, fertilization, synaptic plasticity and information processing. (shawprize.org)

Dear Colleagues, Reproduction is a step by step process that starts at the production of gametes (gametogenesis), proceeds with their activation and interaction (fertilization) and virtually ends when full embryo development leads to the formation of a new adult individual. (mdpi.com)

The exact mechanism for signalling has previously eluded researchers, according to Professor Sheng Luan, chair of the PMB department and senior author on the paper. (nob6.com)

suspect that this unusual swimming behaviour guides sperm through a small hole in the protective coating of fish eggs, which eventually leads to fertilization. (elifesciences.org)

In general, sperm cells become attracted to egg cells by various chemical or physical signals. (elifesciences.org)
In 2009, researchers identified a potassium ion channel, called CNGK, that starts the electrical signal in the sperm cells of sea urchins. (elifesciences.org)
The researchers found that 13, or 45 percent, of the 29 UV filters tested induced calcium ion influxes in the sperm cells, thus interfering with normal sperm cell function. (newswise.com)

The Machaca Lab is interested in intracellular signaling under physiological and pathological conditions with a focus on calcium signaling. (hamad.qa)

This calcium increase is vital for all the early events of fertilization and early embryo development. (biotexcom.com)

By inhibiting the increase in NO at fertilization, we find not that the Ca(2+) transient is abolished but that the duration of the transient is significantly reduced. (ox.ac.uk)
We conclude that NO is unlikely to be a primary egg activator but, rather, acts after the initiation of the Ca(2+) wave to regulate the duration of the fertilization Ca(2+) transient. (ox.ac.uk)

We then tested the effects of various calcium channel blockers to try to better characterize how the signals are generated. (uib.no)

Importantly, it has also emerged that Ca 2+ may even regulate the generation and the transmission of its signal by controlling the expression of its own transporters. (schoolbag.info)
In summary, the method described here provides a convenient and effective tool to study calcium dynamics in a specific subcellular compartment-the sperm acrosome-and how those dynamics regulate the intermediate steps leading to membrane fusion and acrosome exocytosis. (bvsalud.org)
Using tobacco pollen tubes as a model, we addressed the signaling effects of PA by probing the functions of three most relevant enzymes that regulate the production and degradation of PA, namely, phospholipases D (PLD), diacylglycerol kinases (DGKs), and lipid phosphate phosphatases (LPPs). (frontiersin.org)

In particular, the interplay between intra-acrosomal calcium dynamics and the intermediate steps leading to AE is not well-defined. (bvsalud.org)
Here, we describe a method that provides spatial and temporal insights into acrosomal calcium dynamics and their relationship to membrane fusion and subsequent exocytosis of the acrosome vesicle. (bvsalud.org)
Real-time monitoring of acrosomal calcium dynamics and AE in live AcroSensE sperm is achieved using a combination of high frame-rate imaging and a stimulant delivery system that can target single sperm. (bvsalud.org)

Now, eight transporters were found to control the uptake and efflux of calcium. (mdpi.com)

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