Fate differentiation progenitor satellite cell. Medical search. Frequent questions

Muscle satellite cells make up a stem cell populace that is capable of differentiating into myocytes and contributing to muscle mass regeneration upon injury. (healthyguide.info)
The premise behind this manuscript is important and timely for muscle biologists and for stem cell biologists. (sciety.org)
The identification of heterogenous distribution of factors across the myofiber is an important contribution for dissecting how muscle stem cell diversity in a tissue is achieved. (sciety.org)
The quiescent muscle stem cell (QSC) pool is heterogeneous and generally characterized by the presence and levels of intrinsic myogenic transcription factors. (sciety.org)
This series of articles discusses the difficulties we have encountered in adopting stem cell research to clinical therapeutics in regeneration of cardiac tissue damaged post myocardial infarct. (pharmaceuticalintelligence.com)
Enriched expression of genes in LRECb was associated with stem cell attributes and identified WNT, TGF-β, and MAPK pathways of self renewal and proliferation. (frontiersin.org)
whereas LREC in suprabasal epithelial layers are enriched for more committed progenitor cells, expressing some genes that are associated with stem cell attributes along with those indicative of cell differentiation. (frontiersin.org)
Retention of labeled DNA strands may be attributed to the ability of stem cells to retain the parental DNA strand during asymmetric cell division ( Cairns, 1975 ) or to quiescence of the stem cell population such that the DNA label is not diluted by frequent cell divisions ( Klein and Simons, 2011 ). (frontiersin.org)
During periods of low mammary proliferation, quiescence of the stem cell population may account for retention of label. (frontiersin.org)
In the mammalian brain, Notch signaling maintains the cortical stem cell pool and regulates the glial cell fate choice and differentiation. (bvsalud.org)
From a research, medical, and business standpoint, mesenchymal stem cell (MSC)-based therapies are fascinating. (biomedcentral.com)
There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)
In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
He is a member of the Stem Cell Network Centre of Excellence (past member of the Stem Cell Policy Committee and Research Management Committee and current Sub-chair of the Training and Education Committee), and a member of the AllerGen Network Centre of Excellence (Research Management Committee and Co-Chair of the Biomarkers Program). (ubc.ca)
On the cover: Human induced pluripotent stem cell-derived neuroepithelial-like stem cells stained for actin (green) and DNA (blue). (5dok.org)
We used conjugated polymers to develop a novel neural stem cell culture substrate with anchored growth factors to promote cell self-renewal. (5dok.org)
Worldwide, 50,000 hematopoietic stem cell transplant (HSCT) procedures are undertaken annually to replace a person's blood-forming cells - hematopoietic stem cells (HSCs) - to treat blood disorders and cancers, yet two-thirds of patients who need HSCT lack matched donor tissue. (utoronto.ca)
The generation of HSCs has been a long-standing goal of stem cell biologists working in the field of developmental hematopoiesis. (utoronto.ca)
This team, which brings together expertise in stem cell biology, cell biology, developmental signalling pathways, and genetic screens, aims at improving methods to achieve generation of HSC. (utoronto.ca)
Our study has identified a new experimental model for stem cell biology, namely 'cluster 3', as a subpopulation of TBLCs that can be molecularly defined as near totipotent cells. (sciencegate.app)
We hypothesize that the ESC ovarian tumor model, with human vascular cells, will provide an ideal microenvironment to support human stem cell growth. (nih.gov)

Intro The regenerative response of skeletal muscle mass to ICAM4 injury is dependent within the quiescent populace of skeletal muscle mass stem cells termed the satellite cells which reside beneath the basal lamina of each myofiber (Brack and Rando 2012 Wang and Rudnicki 2012 Upon injury these quiescent satellite cells become triggered and undergo proliferation providing rise to myogenic progenitors (MPs) that ultimately differentiate into mature myofibers. (scienceexhibitions.org)

2009 potentially reflecting the reduced secretion of myogenic growth element IGF-1 by these cells (Wynes and Riches 2003 While these studies demonstrate a facilitative part of innate immune cells in muscle mass regrowth after injury a direct molecular link between the innate immune system and muscle mass progenitor biology remains to be founded. (scienceexhibitions.org)
Despite successes in pre-clinical animal models with stem cells, a problem arises with respect to the biology of the transplanted progenitor cells. (pharmaceuticalintelligence.com)
Insights into the biology of stem cells will be gained by confirmation and characterization of candidate MaSC markers identified in this study. (frontiersin.org)
The most prominent example is the changes in cell biology and morphology when it evolves from a healthy to a cancerous state [1, 3]. (globaltechbiz.com)
Seminars in Cell and Developmental Biology, 72. (unibas.ch)
Cold Spring Harbor, NY -- Cold Spring Harbor Laboratory Press (CSHLP) announced the release of The Digital Cell: Cell Biology as a Data Science, available on its website in hardcover format. (cshlpress.com)

Fig. 1: Recapitulation of the mouse and human segmentation clocks in vitro by differentiation of pluripotent stem cells towards PSM fate. (nature.com)
Recently, it was reported that treatment with a single spliceosome inhibitor, pladienolide B (plaB), can successfully reprogram mouse pluripotent stem cells into totipotent blastomere-like cells (TBLCs) in vitro. (sciencegate.app)

Previous molecular characterizations of mammary stem cells (MaSC) have utilized fluorescence-activated cell sorting or in vitro cultivation of cells from enzymatically dissociated tissue to enrich for MaSC. (frontiersin.org)
Here we show that human PSM cells derived in vitro-as well as those of the mouse 4 -recapitulate the oscillations of the segmentation clock. (nature.com)
Single-cell RNA sequencing reveals that mouse and human PSM cells in vitro follow a developmental trajectory similar to that of mouse PSM in vivo. (nature.com)
Because stromal cells can modulate the functionality of myeloid cells in vitro, targeting stromal-myeloid interactions has become an attractive potential therapeutic strategy. (pklab.org)
In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
Using biologically relevant elastic substrates to study cell function in vitro has proven beneficial, as the in vivo microenvironment usually is much softer than rigid plastic dishes. (5dok.org)
Currently, the ability to culture in vitro totipotent cells possessing molecular and functional features like those of an early embryo in vivo has been a challenge. (sciencegate.app)
This study underscores the subtle differences between in vitro derived TBLCs and in vivo mouse early developmental cell stages at the single-cell transcriptomic level. (sciencegate.app)
2- to 4-cell and morula- to blastocyst-stage mouse embryos were cultured for 1 h in tritiated leucine at two specific activities and their subsequent development followed in vitro and in vivo (after transfer to recipients), respectively. (sciencegate.app)

Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)
These approaches result in the loss of all histological information pertaining to the in vivo locale of MaSC and progenitor cells. (frontiersin.org)
Here we show by in vivo fate mapping that brown, but not white, fat cells arise from precursors that express Myf5, a gene previously thought to be expressed only in the myogenic lineage. (bioseek.eu)
However, it is disputed whether TBLCs are 'true' totipotent stem cells equivalent to in vivo two-cell stage embryos. (sciencegate.app)
One challenge with characterizing tumor stem cells has been finding appropriate conditions for in vivo growth. (nih.gov)
We therefore propose (3) to isolate ovarian tumor stem cells and grow them in vivo using the ESC ovarian cancer model. (nih.gov)

Overall the data provide compelling evidence that niche-derived Dll4 and Mib regulate SC fate, however, whether the interaction maintains a continuum of SC fates as concluded by the authors is insufficiently supported by the data provided. (sciety.org)
We find that C/EBPβ can regulate Notch2 expression and that restoration of Notch activity in myoblasts lacking C/EBPβ prevents precocious differentiation. (biomedcentral.com)
CCAAT/enhancer binding proteins (C/EBPs) are a family of transcription factors that regulate cellular growth and differentiation, metabolism, and inflammation [ 18 ]. (biomedcentral.com)
Gain- and loss-of-function experiments exposed that modulated muscle mass dietary fiber types and oxidative rate of metabolism in myotubes, and that stimulated myoblast differentiation, but did not regulate myogenic cell self-renewal. (researchassistantresume.com)
To better understand the mechanisms that regulate Notch function in cancer, we studied Notch signaling in a Drosophila tumor model, prostate cancer-derived cell lines, and tissue samples from patients with advanced prostate cancer. (bvsalud.org)
There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
He performed some of the first studies to identify transcription factors that regulate the gene expression and differentiation of eosinophils, which are known to play a major role in allergic and asthmatic responses. (ubc.ca)
His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
dGLYAT was found to regulate Gadd45 -mediated JNK pathway activation and cell invasion. (sdbonline.org)

Our data shows that a post-translational mechanism controls spatial heterogeneity of Notch ligands in a multinucleated niche cell to maintain a continuum of diverse states within the SC pool during tissue homeostasis. (sciety.org)
The embryonic AMPs are at the origin of all adult fly muscles and, as we demonstrate here, they express repressors of myogenic differentiation and targets of the Notch pathway known to be involved in muscle cell stemness. (biologists.com)
2008 For instance autocrine Notch signaling regulates the activation and proliferation of satellite cells (Bjornson et al. (scienceexhibitions.org)
Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
Here, we show that Notch inactivation by genetic deletion of Rbpj in stem cells decreases astrocytes but increases oligodendrocytes with altered internal states. (bvsalud.org)
Inhibiting Notch in glial progenitors does not affect cell generation but instead accelerates the growth of Notch-deprived oligodendrocyte progenitor cells (OPCs) and OPC-related glioma. (bvsalud.org)
Moreover, Notch inactivation in stem cells causes a glioma subtype shift from astroglia-associated to OPC-correlated patterns and vice versa. (bvsalud.org)
Consequently, inactive Notch accumulated in cells, where it was unable to transcribe genes encoding its target proteins, many of which have tumor-suppressive activities. (bvsalud.org)
The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)
We have therefore investigated the role of matrix stiffness in Notch signaling in breast cancer cells. (5dok.org)
Our results show that we can tune cell stiffness and migration by regulating Notch activity and matrix stiffness. (5dok.org)
We propose an opportunity to target the cancer cell/microenvironment interface instead of the Notch pathway itself in the development of cancer therapies. (5dok.org)

They are integral regulators of essential cell signaling pathways affecting cell growth, proliferation, differentiation, and migration. (wikipedia.org)
Commensurate with the known truth that HuR improved the great quantity of adhesion substances VLA-4 on Th17 cells, knockout of HuR impaired splenic Th17 cell migration towards the central anxious program and abolished the condition. (globaltechbiz.com)
Eph/ephrin signaling is a cell-to-cell communication pathway, which regulates cell migration and proliferation. (5dok.org)
Firstly, loss of dGLYAT suppressed scrib depletion- or Egr overexpression-induced JNK pathway activation and invasive cell migration. (sdbonline.org)
Molecular motors lie at the heart of biological processes from DNA replication to cell migration. (nih.gov)

In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
A new tumor model, combining human embryonic stem cells (ESC) and tumor cells, develops abundant human vessels. (nih.gov)

Upon activation satellite cells rapidly initiate MyoD expression which leads to the activation of myogenin and terminally differentiated structural muscle mass genes such as myosin heavy chain (MHC) [1] [3]. (healthyguide.info)
Genes expressed in LRECe revealed retention of some stem-like properties along with up-regulation of differentiation factors. (frontiersin.org)
Selumetinib causes long-term G1 arrest accompanied by reduced expression of DNA replication and repair genes, but cells stochastically re-enter the cell cycle during treatment despite continued repression of pERK1/2. (babraham.ac.uk)
The group plans to identify better markers to track and purify key cell intermediates as well as uncover key genes/pathways that lead to HSC development. (utoronto.ca)
Differential expression analyses were performed between TBLCs and multi-embryonic cell stages to identify differentially expressed genes. (sciencegate.app)
Remarkably, a subpopulation within the TBLCs population expressed a high level of the totipotent-related genes Zscan4s and displayed transcriptomic features similar to mouse two-cell stage embryonic cells. (sciencegate.app)

Using genetic tools to conditionally abrogate C/EBPβ expression in Pax7 + cells, we examined the role of C/EBPβ in self-renewal of satellite cells during muscle regeneration. (biomedcentral.com)
After a single muscle injury, C/EBPβ-deficient satellite cells fail to self-renew resulting in a reduction of satellite cells available for future rounds of regeneration. (biomedcentral.com)
These findings demonstrate that C/EBPβ is a novel regulator of satellite cell self-renewal during muscle regeneration acting at least in part through Notch2. (biomedcentral.com)
Remarkably type 2 cytokine signaling is also required in FAPs but not myeloid cells for quick clearance of necrotic debris a process that is necessary for timely and total regeneration of BV-6 cells. (scienceexhibitions.org)
2008 In addition to satellite cells recent studies have recognized an important part for fibro/adipogenic progenitors (FAPs) in muscle mass regeneration and its fatty degeneration (Joe et al. (scienceexhibitions.org)
In a number of species cells regeneration is associated with the presence of the molecular signature for type 2 innate immune response such as alternatively triggered (M2) macrophages and eosinophils (Allen and Wynn 2011 Palm et al. (scienceexhibitions.org)
This is Part 3 of a series of contributions on cardiac regeneration after myocardial infarct with stem cells. (pharmaceuticalintelligence.com)
Part I went into some detail on a method of obtaining suitable cells, growing them in sheets, and transferring the sheets to the surface for regeneration and repair, which is now going into clinical trials. (pharmaceuticalintelligence.com)
Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
Skeletal muscle growth and regeneration involves the activity of resident adult stem cells, namely satellite cells (SC). (sdbonline.org)

Finally we found that in an mouse model of fracture healing where muscle mass progenitor cells were lineage-traced Nkx3.2 and Sox9 are significantly upregulated while Pax3 is significantly downregulated in the muscle mass progenitor cells that give rise to chondrocytes during fracture restoration. (healthyguide.info)
Interestingly recent data indicated that although MyoD is not indicated in quiescent satellite cells in the adult it is transiently indicated in satellite cell progenitors in the embryo suggesting that satellite cells are derived from committed embryonic precursors of myogenic lineage [4] [5]. (healthyguide.info)
One such alternative cell fate is the adipogenic fate as Pax7(+) satellite cells isolated from solitary myofibers used adipogenic fate in addition ARHGAP1 to muscle mass fate significance of these factors we used a mouse fracture healing model inside a genetically altered reporter mouse where muscle mass progenitors were lineage-traced. (healthyguide.info)
2010 FAPs which do not arise from your myogenic lineage are bipotential cells capable of providing rise to fibroblasts and adipocytes. (scienceexhibitions.org)
Taken together, these data indicate that PRDM16 specifies the brown fat lineage from a progenitor that expresses myoblast markers and is not involved in white adipogenesis. (bioseek.eu)
The splicing co-factor Barricade/Tat-SF1, is required for cell cycle and lineage progression in Drosophila neural stem cells. (unibas.ch)
In Drosophila with depleted RACK1 in all muscle cells or, specifically, in SC lineage resulted in a delayed recovery of skeletal muscle after physical damage as well as the low presence of active SC in the wound area. (sdbonline.org)

Understanding the mechanisms controling BAT differentiation may identify potential therapeutic targets to enhance BAT-controlled metabolism and to increase EE. (biomedcentral.com)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
Laboratory work has centered on the mechanisms of how cells acquire specific fates during growth and development, providing insights into normal and abnormal mechanisms of growth control. (stanford.edu)
While much is known about biochemical signaling, many of the mechanisms that drive cell outcome in response to biophysical influences remain to be uncovered. (5dok.org)

For example, these manuscripts establish that elevated Pax7 levels drive quiescence and low Pax7 levels correlate with differentiation. (sciety.org)
The transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in Pax7 + satellite cells of healthy muscle and is downregulated during myoblast differentiation. (biomedcentral.com)
Persistent expression of C/EBPβ upregulates Pax7, inhibits MyoD, and blocks myogenic differentiation. (biomedcentral.com)

In living organisms, if the temperature exceeds the normal range (thermo-neutral zone), it disturbs the normal physiological functioning and induces cell injury. (frontiersin.org)
Conversely, ectopic expression of PRDM16 in myoblasts induces their differentiation into brown fat cells. (bioseek.eu)

Postnatal growth and repair of skeletal muscle relies upon a population of quiescent muscle precursor cells, called satellite cells that can be activated to proliferate and differentiate into new myofibers, as well as self-renew to replenish the satellite cell population. (biomedcentral.com)
These suggested that every myogenic cell was predetermined to differentiate into a specific fiber type, and that the recipient muscle mass materials may not impact the fate of injected cells. (researchassistantresume.com)
The developmental potential within pluripotent cells in the canonical model is restricted to embryonic tissues, whereas totipotent cells can differentiate into both embryonic and extraembryonic tissues. (sciencegate.app)

When overexpressed in the murine myoblast cell line C2C12 or in primary myoblasts, C/EBPβ reduced MyoD and other myogenic protein levels during differentiation, in addition to reducing myoblast fusion. (biomedcentral.com)

The balance between differentiation and self-renewal is critical to maintain muscle tissue homeostasis, and alterations in this equilibrium can lead to chronic muscle degeneration. (biomedcentral.com)
We find that loss of C/EBPβ leads to precocious differentiation at the expense of self-renewal in primary myoblast and myofiber cultures. (biomedcentral.com)
Loss of C/EBPβ in SCs results in precocious differentiation at the expense of self-renewal in both primary myoblasts and myofiber cultures. (biomedcentral.com)

WAT is characterised by its capacity to adapt and expand in response to surplus energy through processes of adipocyte hypertrophy and/or recruitment and proliferation of precursor cells in combination with vascular and extracellular matrix remodelling. (springer.com)
Analysis of supporting stromal cells within the tumor environment demonstrates vascular remodeling within the endothelial cells. (pklab.org)
Finally, our preliminary data suggest that the ESC-ovarian tumor model has human tumor vascular cells. (nih.gov)
Tumor vascular cells are critical for the growth of tumor stem cells, which reside within the vascular niche. (nih.gov)

Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
Interest in ALMS1 is heightened by the recent discovery of its involvement in neonatal cardiomyocyte cell cycle arrest, a process with potential relevance to regenerative medicine. (springer.com)
The use of mesenchymal stem cells (MSCs) as a regenerative therapy holds the promise of regrowing injured vasculature, helping DM patients live healthier and longer lives. (thno.org)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
An increasing number of studies have demonstrated that EPCs are of great value in regenerative medicine and clinical applications, which can not only directly participate in angiogenesis but also participate in vascularization and endothelial repair by differentiating into endothelial cells [ 7 ]. (biomedcentral.com)

Satellite cells from your trunk and the limb are derived from an embryonic populace of progenitor cells in the somites transient mesodermal constructions that develop on either part of the neural tube [1]. (healthyguide.info)
sox10 is necessary for development of neural and pigment cell derivatives of the neural crest (NC). (silverchair.com)

Therefore our and analyses suggest that the balance of Pax3 Nkx3.2 and Sox9 may act as a molecular Talniflumate switch during the chondrogenic differentiation of muscle mass progenitor cells which may be important for fracture healing. (healthyguide.info)
Collectively our data suggest that the balance of Nkx3.2 Sox9 and Pax3 can act as a molecular switch during the chondrogenic differentiation of satellite cells which may play an important part in the healing process experiments were normalized to GAPDH. (healthyguide.info)
Instead, we used laser microdissection to excise putative progenitor cells and control cells from their in situ locations in cryosections and characterized the molecular properties of these cells. (frontiersin.org)
Our results support the use of DNA label retention to identify MaSC and also provide a molecular profile and novel candidate markers for these cells. (frontiersin.org)
These changes take place on the molecular level impacting properties of specific the different parts of cell inner structure, but resulting in alterations in mechanical properties of the complete cell ultimately. (globaltechbiz.com)
MSCs can be defined by their ability to adhere to tissue culture plastic, their expression of several cell surface molecular epitopes-cluster of differentiation CD73, CD90, and CD105, and others-as well as their lack of several surface markers, including CD45 [ 6 ]. (biomedcentral.com)
Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)

conclude that Dll4 and Mib present on myofibers maintain a continuum of SC fates providing SCs capable of regenerating muscle and repopulatin the SC niche. (sciety.org)

Enormous problems have been encountered in the selection of progenitor cells, the growth into compatible and functional myocardial tissue, and the survival of the myocardium. (pharmaceuticalintelligence.com)
The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)
Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)
Our data suggest that metabolic and myogenic properties of myogenic progenitor cells vary depending on the type of muscle mass from which they originate, and that manifestation partially clarifies the practical variations of myogenic cells derived from fast-type and slow-type muscle tissue. (researchassistantresume.com)
We provide a comprehensive immune cell landscape and characterize cell-state changes from normal tissue to NB. (pklab.org)
In contrast to adult cancers and previous NB studies, we show an increase in inflammatory monocyte cell state when contrasting normal and tumor tissue, while no differences in cytotoxicity and exhaustion score for T cells, nor in Treg activity, are observed. (pklab.org)
The response of target cells is further regulated by their microenvironment, involving the extracellular matrix, which may be modified by MSC-produced matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs. (biomedcentral.com)
Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
The levels of catalase (CAT) and glutathione (GSH) were measured in PC12 cells and Drosophila brain tissue. (sdbonline.org)
GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
Quantitative evaluation of tissue-processing procedures has permitted selection of conditions which reduce changes in linear dimensions to −1·6 ± 1·8 % in two-cell embryos. (sciencegate.app)

Recent mechanistic insights into the trophic activities of these cells point to ultimate regulation by nitric oxide, nuclear factor-kB, and indoleamine, among other signaling pathways. (biomedcentral.com)

The close association of FAPs with regenerating muscle mass fibers along with their manifestation of factors that influence myogenic differentiation such as IL-6 and IGF-1 suggests that these stromal cells may perform a supportive part in myogenic differentiation (Joe et BV-6 al. (scienceexhibitions.org)
We aimed to investigate how human liver stromal cells impact myeloid cell properties and to understand the utility of a stromal-myeloid coculture system to study these interactions in the context of cirrhosis. (pklab.org)

European Blot analysis For European Blot analysis total protein lysates were obtained following a standard protocol from confluent 6 cm cells culture plates comprising roughly 3×106 cells [25]. (healthyguide.info)

2012 whereas paracrine actions of IL-6 and insulin-like growth factors (IGFs) have been implicated in the differentiation of MPs into mature myotubes (Bodine et al. (scienceexhibitions.org)
was highly indicated in TA-MBs, and found that modulated muscle mass rate of metabolism in myotubes and myogenic differentiation in myoblasts. (researchassistantresume.com)
These observations highlighted the practical differences of sluggish- and fast-type muscle-derived satellite cells and suggested in myotubes were quantified by qPCR. (researchassistantresume.com)
The dynamics of RACK1 levels in isolated adult SC of mice, i.e., progressively high during differentiation and low compared to proliferating conditions, and RACK1 silencing indicated that RACK1 promotes both the formation of myotubes and the accretion of nascent myotubes. (sdbonline.org)

These cells are regulated by a tight control of transcriptional programs. (biomed.news)
We also demonstrate that the transcriptional regulator PRDM16 (PRD1-BF1-RIZ1 homologous domain containing 16) controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells. (bioseek.eu)
Single-cell transcriptomic analysis of tumor cells from the primary tumors reveals a distinct transcriptional signature that is predictive of metastatic potential and patient survival. (pklab.org)

Here, we perform single-cell RNA sequencing (scRNA-seq) on 19 human NB samples coupled with multiplex immunohistochemistry, survival analysis, and comparison with normal fetal adrenal gland data. (pklab.org)
Several different cell types demonstrate a survival benefit. (pklab.org)
In clinical practice, these involve the life-long immunosuppression of the organ transplantation patients, the creation of the supporting environment for engineered tissues in the mended organ, the unsolved issues of cell survival and differentiation of the cell-based therapy, and the selection and development of vectors for gene therapy. (biomedcentral.com)

This signaling pathway is a highly conserved cell-to-cell communication system that regulates cell fate in development and disease. (5dok.org)

Heparan sulfate proteoglycans (HSPGs) act as co-receptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. (wikipedia.org)
Upon injury or when challenged with a variety of mechanical or biochemical stimuli satellite cells re-enter the cell cycle and give rise to Talniflumate differentiated myocytes which form new muscle mass materials or fuse with existing materials and contribute to muscle mass growth and restoration [1]. (healthyguide.info)
This dictates cycles of mammary growth, differentiation, lactation, and regression, during which mammary stem cells (MaSC) provide for the lineages of luminal and basal (myoepithelial) epithelial cells in the ducts and alveoli. (frontiersin.org)
During rapid mammary growth in the mouse, label retaining epithelial cells (LREC) appear to retain label by asymmetric distribution of DNA strands, as evidenced by a rapid proliferation index of the LREC ( Smith, 2005 ). (frontiersin.org)
We confirmed that typical growth factor signaling was in fact occurring in muscle cells exposed to hESC produced factors, and that hESCs produce a TGF-beta antagonist. (ca.gov)
Upon an electrochemical switch, growth factor presentation was reversed, which initiated cellular differentiation along the neuronal lineages. (5dok.org)
Recombinant production of growth factors for application in cell culture. (au.dk)

Intro Satellite cells are the cells specific stem cells in the adult skeletal muscle mass. (healthyguide.info)
In the beginning satellite cells were considered to Talniflumate be unipotent stem cells with the ability of generating a unique specialized phenotype the skeletal muscle mass cells. (healthyguide.info)

The function of HSPGs extends beyond providing an extracellular matrix (ECM) structure and scaffold for cells. (wikipedia.org)
this was the first evidence that an extracellular sulf was capable of modifying HS and therefore cell signaling. (wikipedia.org)
Semaphorins are a wide family of extracellular regulatory signals with a multifaceted role in cell-cell communication. (biomed.news)
These MSC influences can range from relatively rare activities that require cell contact, such as mitochondrial transfer and cell fusion, to relatively common paracrine MSC actions through extracellular microvesicles or secreted factors. (biomedcentral.com)
Cells sense and respond to the mechanics of their substrate - be it the extracellular matrix, neighboring cells or artificial matrix in cell culture. (5dok.org)

Our findings provide biological insights into the interplay between tumor cells and the ccRCC microenvironment. (pklab.org)
Analysis of 22 tumor samples, including multiple myeloma, gastric, breast and thyroid cancers, shows that Numbat can reconstruct the tumor copy number profile and precisely identify malignant cells in the tumor microenvironment. (pklab.org)
If successful, this will create a murine tumor model that nearly completely reproduces the human tumor microenvironment with human tumor stroma, vessels and tumor stem cells. (nih.gov)

Single-cell RNA sequencing (scRNA-seq) has the potential to investigate both genetic and nongenetic sources of tumor heterogeneity in a single assay. (pklab.org)
Numbat exploits the evolutionary relationships between subclones to iteratively infer single-cell copy number profiles and tumor clonal phylogeny. (pklab.org)
The intent of vaccination is to induce a combined antibody and T-cell anti-HER-2 immune response, which is intended to target HER-2-expressing tumor cells, and may induce tumor regression or slow progression of disease. (stanford.edu)
The primary purpose of this study is to determine whether breast cancer tumors respond (as measured by pathologic complete response: the absence of microscopic evidence of invasive tumor cells in the breast) to combined chemotherapy of AC(doxorubicin and cyclophosphamide) followed by paclitaxel plus trastuzumab or lapatinib or both given before surgery to patients with HER2-positive breast cancer. (stanford.edu)
RATIONALE: Drugs used in chemotherapy use different ways to stop tumor cells from dividing so they stop growing or die. (stanford.edu)
Cell invasion is a crucial step of tumor metastasis , finding new regulators of which offers potential drug targets for cancer therapy. (sdbonline.org)

Macrophage activation has been described as a dynamic process: the same cell may initially induce proinflammatory and cytotoxic reactions and later may take part in the resolution of inflammation and wound healing [ 9 ]. (hindawi.com)

Thus, alternative sources of patient-specific hematopoietic stem cells (HSCs) are required. (utoronto.ca)

For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)
These cells are located beneath the basal lamina of myofiber in a dormant state. (elifesciences.org)

Activation of IL-4/IL-13 signaling promotes proliferation of FAPs to support myogenesis while inhibiting their differentiation into adipocytes. (scienceexhibitions.org)
Loss of PRDM16 from brown fat precursors causes a loss of brown fat characteristics and promotes muscle differentiation. (bioseek.eu)

The cell membrane is really a viscous fluid-like matter which includes several lipids, cholesterol, and inserted proteins. (globaltechbiz.com)
In the next reporting period we will re-confirm that the levels of candidate proteins from the 500 antibody array actually are very highly produced by hESCs and that the signals from these proteins are perceived by regenerating muscle cells. (ca.gov)
In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)

a reverse function mutant Talniflumate of Nkx3.2 blocks the ability of Sox9 to both inhibit myogenesis and induce chondrogenesis suggesting that Nkx3.2 is required for Sox9 to promote chondrogenic differentiation in satellite cells. (healthyguide.info)
We hypothesize that ovarian cancer cells will induce human ovarian TVM expression. (nih.gov)

Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)
We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)
Concomitantly, the level of transcription from major satellite decreases. (sciencegate.app)

Alternatively activated M2 macrophages comprise cells exposed to low concentrations of M-CSF in the presence of IL-4, IL-13, or IL-10. (hindawi.com)

Adult mesenchymal stem cells (MSCs) represent a subject of intense experimental and biomedical interest. (biomedcentral.com)

Here we have investigated biophysical regulation of cell function. (5dok.org)

Despite this, clinical responses are typically transient as tumour cells develop resistance. (babraham.ac.uk)

The manipulation of fat stores is an obvious therapeutic dream, but disruption of the normal differentiation or development of white adipose tissues (WAT) causes ectopic lipid storage and severe pathology (lipodystrophy) in both humans and experimental animals. (bioseek.eu)
An in silico cell-to-cell interaction analysis highlights the CXCL9/CXCL10-CXCR3 axis and the CD70-CD27 axis as potential therapeutic targets. (pklab.org)

To address this question, single-cell RNA sequencing was applied to TBLCs and cells from early mouse embryonic developmental stages and the data were integrated using canonical correlation analyses. (sciencegate.app)

Anatomy64

Organisms16

Diseases8

Chemicals and Drugs80

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes53

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science6

Health Care2

Stem cell22

Myogenic progenitors1

Biology6

Pluripotent stem2

Vitro9

VIVO6

Regulate9

Notch12

Migration5

Embryonic stem2

Genes6

Regeneration14

Lineage7

Mechanisms4

Pax73

Induces2

Differentiate3

Myoblast fusion1

Self-renewal3

Vascular4

Regenerative5

Neural2

Molecular7

Myofibers1

Tissue11

Pathways1

Stromal cells2

Protein1

Myotubes4

Transcriptional3

Survival3

Regulates1

Growth7

Skeletal muscle mass2

Extracellular5

Microenvironment3

Tumor6

Inflammation1

Hematopoietic stem1

Located beneath the basal lamina2

Promotes2

Proteins4

Induce2

Transcription3

Macrophages1

Mesenchymal stem1

Regulation1

Transient1

Therapeutic2

Single-cell RNA1