Cerulenin, a fatty acid synthase (FASN) natural inhibitor, and Atorvastatin, a competitive inhibitor of the mevalonate pathway, have been used to test the cytotoxic effects on PDAC CSCs. (atlasofscience.org)
We observed a highly up-regulated fatty acid synthase (FASN) level in A549CisR and H157CisR cells compared to parental cells and the up-regulation of FASN was also detected in A549P and H157P cells after short time treatment with cisplatin, suggesting that the high level of FASN in cisplatin-resistant cells may be from the accumulated cellular responses during cisplatin-resistance developmental process. (oncotarget.com)
Expression2
Mechanistically, deletion of Bmal1 abrogates expression of its transcriptionally targeted plasminogen activator inhibitor-1 (PAI-1). (bvsalud.org)
No significant tumor regression was detected at the end of cerulenin treatment, but IHC staining showed higher expression of EMT/metastasis markers in H157CisR cell-derived tumors than H157P cell-derived tumors, and showed dramatic reduction of these markers in tumor tissues of cerulenin-treated mice, confirming the in vitro results. (oncotarget.com)
Treatment4
Cerulenin or orlistat treatment decreased cells proliferation, accompanied by increased amounts of the tumor suppressor protein p21WAF1/Cip1, as well as induced apoptosis, but not necrosis, in melan-a cell line. (unicamp.br)
siRNAi for FASN did not culminate in apoptosis, and FASN inhibitors treatment did not alter free fatty acids content in the non-tumorigenic cells, as verified by mass spectrometry, suggesting that cerulenin or orlistat induces apoptosis independent on FASN inhibition. (unicamp.br)
Respiratory inhibition after cerulenin or orlistat treatment, respectively, was restricted to the oxidation of NADH-linked substrates (39.9 or 60,8%) and succinate (45.8 or 51.8%) and was not significant when mitochondria were respiring on the complex IV substrate, N,N,N? (unicamp.br)
Second, we demonstrated that treatment with the FAS inhibitor, cerulenin (Cer), significantly decreased meningioma cell survival in vitro. (wustl.edu)
Cell1
Here we investigate the effects of these inhibitors on the non-tumorigenic mouse cell line melan-a. (unicamp.br)