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Protein BiosynthesisProtein BindingProtein Interaction MappingMolecular Sequence DataTwo-Hybrid System TechniquesAmino Acid SequenceBinding SitesModels, MolecularProtein Structure, TertiaryBase SequencePeptide Elongation Factor TuProtein ConformationRibosomesRNA, Transfer, Amino AcylEscherichia coliPeptide Chain Elongation, TranslationalTranscription FactorsAbrinPeptide Elongation FactorsCell LineRNA, TransferGeobacillusAmino Acyl-tRNA SynthetasesBacterial ProteinsRibosomal ProteinsPeptide Chain Initiation, TranslationalPoly UKineticsTransfer RNA AminoacylationRNA, MessengerKeratins, Type IICycloheximideGlutamate-tRNA LigasePeptide Elongation Factor GBiosynthetic PathwaysGTP Phosphohydrolase-Linked Elongation FactorsProtein Synthesis InhibitorsRNA, Transfer, ArgMutationGenetic CodeMethionine-tRNA LigasePhenylalanineRNA, Transfer, PhePeptide BiosynthesisPeptide Chain Termination, TranslationalPeptide Elongation Factor 2Gene Expression ProfilingMethionineCell-Free SystemSpiro CompoundsPyridonesOligonucleotide Array Sequence AnalysisCloning, MolecularPeptide Elongation Factor 1Leucine-tRNA LigaseGuanosine TriphosphateLeucineAmino AcidsNucleic Acid ConformationRNA, BacterialStreptomycesGene Expression Regulation, PlantMolecular StructureAnticodonSequence Homology, Amino AcidElectrophoresis, Polyacrylamide GelTranscription, GeneticRNAPuromycinSaccharomyces cerevisiaeGene Expression Regulation, BacterialCarbon RadioisotopesReticulocytesPeptide Initiation FactorsSubstrate SpecificityRNA, RibosomalTritiumPolyribosomesFungal ProteinsLiverMultigene FamilyAnti-Bacterial AgentsGuanine NucleotidesProteomeEscherichia coli ProteinsModels, BiologicalGene ExpressionMass SpectrometryGuanosine DiphosphateGenes, BacterialPeptidesDNA, ComplementaryGene Expression RegulationReverse Transcriptase Polymerase Chain ReactionMolecular WeightDNA PrimersTime FactorsGenetic Complementation TestElectrophoresis, Gel, Two-DimensionalTerpenes
Cofactor biosynthesis proteinsKinaseGlycosylphosphatidylinositolKinasesPolyketideEnzymesMolybdopterinAminoMetabolismGlycanPutativeDistribution of proteinsStructurallyBacterialEndoplasmicPeptidesRegulationTranscription factorsHuman RecombinantMutationsVitroProkaryoticHighly conservedCompoundsAssaysSequencesSuperfamilyRibosomalSpeciesMembrane proteinCrucial roleMRNAAntimicrobialEnzymeCrystal StructureClassificationInteractAnchorRolesRibosomeKEGGBelongsExtracellularCatalyzesHormoneSecondaryCellFoundSimilarMolecularDomainRegulatorsGenes involvedDomainsRepressor
Cofactor biosynthesis proteins1
  • Variant MobA proteins were characterized for their ability to support the synthesis of active molybdenum enzymes, to bind MPT and MGD, to interact with the molybdenum cofactor biosynthesis proteins MobB and MoeA. (rcsb.org)
Kinase7
  • Prokaryotic riboflavin biosynthesis proteins are also known as the prokaryotic type-I FAD synthetases, which consist of a C-terminal riboflavin kinase (RFK) and an N-terminal FMN-adenylyltransferase (FMNAT). (wikipedia.org)
  • Our lab seeks to understand how the protein kinase family organises complex intracellular communication networks. (edu.au)
  • We employ a multidisciplinary approach including kinase biochemistry and biology, structural biology (X-ray crystallography and cryo-electron microscopy), assay development, high-throughput screening, proteomics, imaging and chemical biology to gain high-resolution insights into kinase/pseudokinase signaling proteins. (edu.au)
  • As this interaction establishes a new link between the mitogenic cascade and protein biosynthesis, in vitro kinase assays were performed in presence of B- and C-RAF kinase and recombinant EF1A1/2 to investigate this important interaction. (unina.it)
  • The transcription factor FOSL1 (also known as Fra-1), which is found in basal-like breast cancers but not in the luminal subtype, stimulated the transcription of miR-221/222, and the abundance of these miRNAs decreased with inhibition of the epidermal growth factor receptor (EGFR) or MEK (mitogen-activated or extracellular signal-regulated protein kinase kinase), placing miR-221/222 downstream of the RAS pathway. (nih.gov)
  • In his research Dr. Budanov and his collaborators have demonstrated that the major activity of Sestrins involves the regulation of mammalian Target-of-Rapamycin (mTOR) kinase, the critical controller of protein and lipid biosynthesis, cell growth and metabolism. (skoltech.ru)
  • As they described, Sestrins work via activation of AMPK kinase and TSC1:TSC2 protein complex. (skoltech.ru)
Glycosylphosphatidylinositol1
  • This gene encodes an enzyme that catalyzes the second step of glycosylphosphatidylinositol (GPI) biosynthesis, which is the de-N-acetylation of N-acetylglucosaminylphosphatidylinositol (GlcNAc-PI). (ucsc.edu)
Kinases2
  • This bacterial protein is functionally similar to the monofunctional riboflavin kinases and FMN-adenylyltransferases of eukaryotic organisms, but only the riboflavin kinases are structurally homologous. (wikipedia.org)
  • Members of the src-family tyrosine kinases that are activated during the transition from G2 PHASE to M PHASE of the CELL CYCLE. (bvsalud.org)
Polyketide2
  • This cupin signature is found as a central component of many microbial proteins including certain types of phosphomannose isomerase, polyketide synthase, epimerase, and dioxygenase. (embl.de)
  • These enzymes are found in a variety of biosynthetic contexts, which include fatty-acid activating enzymes, type I or type III polyketide synthases, dialkylresorcinol-generating enzymes, monooxygenases or Rieske proteins. (biomedcentral.com)
Enzymes3
  • ATP + riboflavin ⇌ ADP + FMN ATP + FMN ⇌ diphosphate + FAD Eukaryotes usually have two separate enzymes, while most prokaryotes have a single bifunctional protein that can carry out both catalyses, although exceptions occur in both cases. (wikipedia.org)
  • Phylogenetic profiling (comparison of differential gene content) based on F(420) biosynthesis nominated many actinobacterial proteins as candidate F(420)-dependent enzymes. (umd.edu)
  • Significantly, these results showed that M. tuberculosis carries at least 28 separate F(420)-dependent enzymes, most of unknown function, and a paucity of flavin mononucleotide (FMN)-dependent proteins in these families. (umd.edu)
Molybdopterin1
  • MGD is synthesized from molybdopterin (MPT) and GTP in a reaction catalyzed by the MobA protein. (rcsb.org)
Amino4
  • To study structure-function relationships, we have constructed a number of site-specific mutations of the most highly conserved amino acid residues of the MobA protein family. (rcsb.org)
  • This gene encodes a member of the aromatic amino acid hydroxylase family. (nih.gov)
  • Knowledge of the three-dimensional structure of two vicilins, seed proteins with a characteristic beta-barrel core, led to the identification of a small number of conserved residues and thence to the discovery of several microbial proteins which share these key amino acids. (embl.de)
  • Here, the amino acid sequences of eryngitin 3 and 4, RL-Ps isolated from Pleurotus eryngii fruiting bodies, were determined to (i) obtain structural information on this specific ribonuclease family from edible mushrooms and (ii) explore the structural determinants which justify their different biological and antipathogenic activities. (bvsalud.org)
Metabolism2
  • Sestrins are a family of proteins that play key roles in regulating aging and metabolism. (skoltech.ru)
  • Sestrins are a family of stress-responsive genes involved in the regulation of cell viability and metabolism. (skoltech.ru)
Glycan1
  • Homo sapiens phosphatidylinositol glycan anchor biosynthesis class L (PIGL), mRNA. (ucsc.edu)
Putative3
  • Crystal structure of the putative cell-wall lipoglycan biosynthesis protein LmcA from Mycobacterium smegmatis. (edu.au)
  • Among several EPS-defective mutants, we identified a mutant, SL341P4, with a Tn5-insertion in a gene encoding a putative NDP-sugar epimerase, a putative membrane protein with sugar-modifying moiety, in a reverse orientation to EPS biosynthesis gene cluster. (ppjonline.org)
  • Identification of putative novel class-I lanthipeptides in firmicutes: A combinatorial in silico analysis approach performed on genome sequenced bacteria and a close inspection of Z-geobacillin lanthipeptide biosynthesis gene cluster of the Thermophilic geobacillus sp. (lu.se)
Distribution of proteins3
  • Fluorescence-tags, commonly used to visualize the spatial distribution of proteins within cells, can influence the localization of the tagged proteins by affecting their stability, interaction with other proteins or the induction of oligomerization artifacts. (nature.com)
  • Taxonomic distribution of proteins containing PlsC domain. (embl-heidelberg.de)
  • Taxonomic distribution of proteins containing JmjC domain. (embl.de)
Structurally1
  • 1999). TCP genes encode structurally related proteins that are implicated in the evolution of key morphological traits (Li, 2015). (grassius.org)
Bacterial6
  • To circumvent these obstacles, a protocol was developed to generate 50 nm thick serial sections suitable for immunogold labeling and subsequent reconstruction of the spatial distribution of immuno-labeled native proteins within individual bacterial cells. (nature.com)
  • Intriguingly, helical localization patterns were reported for many other fluorescently tagged bacterial proteins (e.g. chemoreceptors, secretion proteins, RNase E, and the chromosome partitioning protein SetB) but often the questions remains how well these observations reflect the true situation 6 . (nature.com)
  • To circumvent this problem, we have developed a non-invasive protocol enabling the three-dimensional (3D-) visualization of proteins in serial sections of bacterial cells by immunofluorescence microscopy or immunogold labeling envisioned by electron microscopy. (nature.com)
  • However, at present no ssEM protocol was available to investigate protein localization in entire bacterial cells. (nature.com)
  • As well as these single-domain cupins, this survey has identified other classes of two-domain bicupins including bacterial gentisate 1, 2-dioxygenases and 1-hydroxy-2-naphthoate dioxygenases, fungal oxalate decarboxylases, and legume sucrose-binding proteins. (embl.de)
  • Proteins of the MucR/Ros family play a crucial role in bacterial infection or symbiosis with eukaryotic hosts. (bvsalud.org)
Endoplasmic2
  • Study of a similar rat enzyme suggests that this protein localizes to the endoplasmic reticulum. (ucsc.edu)
  • They have been defined as monotropic integral membrane proteins located primarily in the endoplasmic reticulum (COX-1) and the perinuclear envelope (COX-2). (medscape.com)
Peptides1
  • Recently, LC-MS/MS approaches have also been applied to the identification of proteins and proteotypic peptides of lactic acid bacteria in fermented food matrices. (bvsalud.org)
Regulation1
Transcription factors3
  • The JmjN and JmjC domains are two non-adjacent domains which have been identified in the jumonji family of transcription factors. (embl.de)
  • Evidence of domain swapping within the jumonji family of transcription factors. (embl.de)
  • The TCP family of transcription factors were named after the first four characterized members, including teosinte branched1 ( TB1, ZmTCP1 ) from maize ( Zea mays ), cycloidea ( CYC ) from snapdragon ( Antirrhinum majus ) and proliferating cell factors 1 and 2 ( PCF1 and PCF2 ) from rice ( Oryza sativa ) (Luo et al. (grassius.org)
Human Recombinant1
  • Full length human recombinant protein of human GCH1 (NP_001019195) produced in E.coli. (origene.com)
Mutations3
  • [ 4 ] Mutations have been identified throughout the gene, affecting all domains of the transcribed protein, most frequently the catalytic domain. (medscape.com)
  • Mutations of chronic granulomatous disease in Turkish families. (lu.se)
  • Nonsense mutations of the CYBB gene in two Thai families with X-linked chronic granulomatous disease. (lu.se)
Vitro1
  • Applying this method, we show a cellular distribution of the staphylococcal alkaline shock protein 23 (Asp23), which is compatible with filament formation, a property of Asp23 that we also demonstrate in vitro . (nature.com)
Prokaryotic2
  • The prokaryotic riboflavin biosynthesis protein is a bifunctional enzyme found in bacteria that catalyzes the phosphorylation of riboflavin into flavin mononucleotide (FMN) and the adenylylation of FMN into flavin adenine dinucleotide (FAD). (wikipedia.org)
  • While eukaryotic monofunctional RFK is orthologous to the bifunctional prokaryotic RFK module, the monofunctional FMNAT differs from its prokaryotic counterpart, and is instead related to the PAPS-reductase family. (wikipedia.org)
Highly conserved1
  • TCP proteins are characterized by a highly conserved, approximately 60 residue TCP domain in the N-terminal region, which contains a basic helix-loop-helix (bHLH) structure involved in DNA binding and protein interactions (Cubas et al. (grassius.org)
Compounds1
  • Two decades later, the prostaglandins were deduced to be a family of related compounds that contain 20-carbon polyunsaturated fatty acids with a cyclopentane ring, as depicted below. (medscape.com)
Assays1
  • Factor assays in family members are indicated to identify other affected individuals. (medscape.com)
Sequences1
  • We, thus, analyze sequences recognized by MucR in the rem gene promoter, showing that this protein recognizes AT-rich sequences and does not require a consensus sequence to bind DNA. (bvsalud.org)
Superfamily3
  • A domain family that is part of the cupin metalloenzyme superfamily. (embl.de)
  • Microbial relatives of the seed storage proteins of higher plants: conservation of structure and diversification of function during evolution of the cupin superfamily. (embl.de)
  • This review summarizes the recent discovery of the cupin superfamily (from the Latin term "cupa," a small barrel) of functionally diverse proteins that initially were limited to several higher plant proteins such as seed storage proteins, germin (an oxalate oxidase), germin-like proteins, and auxin-binding protein. (embl.de)
Ribosomal2
Species4
  • The DDN family was determined to be limited to F(420)-producing species. (umd.edu)
  • The LLM and PPOX families were observed in F(420)-producing species as well as species lacking F(420) but were particularly numerous in many actinobacterial species, including M. tuberculosis. (umd.edu)
  • Disintegrin-like proteins are found in various species ranging from slime mold to humans. (embl.de)
  • Taken together, our studies establish MucR from Sinorhizobium meliloti as a member of a new family of Histone-like Nucleoid Structuring (H-NS) proteins, thus explaining the multifaceted role of this protein in many species of alpha-proteobacteria. (bvsalud.org)
Membrane protein1
  • Single-pass membrane protein (By similarity). (ucsc.edu)
Crucial role1
MRNA2
  • The transcription of COX-1 yields a 2.7-kilobase (kb) messenger ribonucleic acid (mRNA) that encodes a 576-residue, 65-kd protein. (medscape.com)
  • Conversely, the transcription of COX-2 yields a 4.5-kb mRNA that encodes a 70-kd protein with roughly 70-75% homology to the COX-1 protein. (medscape.com)
Antimicrobial1
Enzyme2
  • The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. (origene.com)
  • Lead's high affinity for sulfhydryl groups makes it particularly toxic to multiple enzyme systems including heme biosynthesis. (cdc.gov)
Crystal Structure1
  • Based on the crystal structure of JmjC domain containing protein FIH and JHDM3A/JMJD2A, the JmjC domain forms an enzymatically active pocket that coordinates Fe(III) and alphaKG. (embl.de)
Classification2
Interact2
  • This action blocks protein synthesis because the damaged ribosomes are unable to interact with elongation factors. (bvsalud.org)
  • These include lead's ability to inhibit or mimic the actions of calcium (which can affect calcium-dependent or related processes) and to interact with proteins (including those with sulfhydryl, amine, phosphate, and carboxyl groups) [ATSDR 2010]. (cdc.gov)
Anchor2
Roles1
  • Anchored proteins have a variety of roles, including sticking cells to one another, relaying signals into cells, and protecting cells from destruction. (medlineplus.gov)
Ribosome3
  • EF1A belongs to the GTP-binding family of proteins and is able to deliver the aa-tRNA to the A-site of the ribosome because of its GTPase activity. (unina.it)
  • Mitochondrial Ribosome Recycling Factor (MRRF) is a member of the RRF family. (prospecbio.com)
  • MRRF controls the release of ribosome from messenger RNA at the termination of protein biosynthesis. (prospecbio.com)
KEGG2
  • This information is based on mapping of SMART genomic protein database to KEGG orthologous groups. (embl-heidelberg.de)
  • Percentage points are related to the number of proteins with PlsC domain which could be assigned to a KEGG orthologous group, and not all proteins containing PlsC domain. (embl-heidelberg.de)
Belongs1
Extracellular1
  • Mammalian cells proteolytically release (shed) the extracellular domains of many cell-surface proteins. (embl.de)
Catalyzes1
  • The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. (nih.gov)
Hormone1
  • Jasmonate acid (JA) is a vital endogenous hormone that regulates taxol biosynthesis and other biological processes, especially (a)biotic stress tolerance in various plants ( Roberts, 2001 ). (frontiersin.org)
Secondary1
  • MYC2 is one of the key regulators of JA signal transfer and the biosynthesis of various secondary metabolites. (frontiersin.org)
Cell6
  • Using yellow fluorescence protein (YFP) tagged MreB, it was initially suggested that MreB forms a continuous, cell-spanning helical filament running along the inner membrane of the cell 4 . (nature.com)
  • Therefore, to challenge the feasibility of this approach, we used the Staphylococcus aureus alkaline shock protein 23 (Asp23) as a model to gain a deeper insight into the spatial organization of Asp23 within the cell. (nature.com)
  • For the S. aureus Asp23 protein a critical function in cell envelope homeostasis was shown 8 . (nature.com)
  • The PEAK family of pseudokinases, their role in cell signalling and cancer. (edu.au)
  • The initial members of the TCP family were implicated in affecting cell division and morphological traits. (grassius.org)
  • DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)
Found4
  • The two major glycoprotein families (DGI and DGII/III) were both found to be synthesized with co-translational addition of 2-4 high mannose cores later processed into complex type chains. (rupress.org)
  • Cubas P, Lauter N, Doebley J, Coen E. The TCP domain: a motif found in proteins regulating plant growth and development. (grassius.org)
  • TcMYC2a was also found to bind with TcJAZ3 in yeast, which was a homolog of Arabidopsis JASMONATE ZIM-domain JAZ proteins, indicating that TcMYC2a had a similar function to AtMYC2 of JA signal transduction. (frontiersin.org)
  • Ribotoxin-like proteins (RL-Ps) are specific ribonucleases found in mushrooms that are able to cleave a single phosphodiester bond located in the sarcin-ricin loop (SRL) of the large rRNA. (bvsalud.org)
Similar3
  • 2023). Class I TCP proteins recognize the consensus binding sequence GGNCCCAC, whereas class II proteins prefer the rather similar sequence GGGNCCAC and the different specificity of both classes has been attributed to changes in the identity of a specific residue located in the basic region (Viola et al. (grassius.org)
  • This protein showed similar to other NDP-sugar epimerases involved in EPS biosynthesis in many phytopathogens. (ppjonline.org)
  • The results further reveal that allicin and polysulfanes have similar modes of actions and thiol-reactivities and modify a similar set of redox-sensitive proteins by S-thioallylation. (cdc.gov)
Molecular1
  • Extracts of metabolically labeled cultured epithelial cells have been analyzed by immunoprecipitation followed by SDS-PAGE, using antisera to the major high molecular mass proteins and glycoproteins (greater than 100 kD) from desmosomes of bovine muzzle epidermis. (rupress.org)
Domain2
  • The complete taxonomic breakdown of all proteins with PlsC domain is also avaliable . (embl-heidelberg.de)
  • Click on the protein counts, or double click on taxonomic names to display all proteins containing PlsC domain in the selected taxonomic class. (embl-heidelberg.de)
Regulators1
  • All these results revealed that TcMYC2a can regulate taxol biosynthesis either directly or via ERF regulators depending on JA signaling transduction. (frontiersin.org)
Genes involved1
  • Chai W, Jiang P, Huang G, Jiang H, Li X. Identification and expression profiling analysis of TCP family genes involved in growth and development in maize. (grassius.org)
Domains3
Repressor1
  • Furthermore, miR-221/222-mediated reduction in E-cadherin abundance depended on their targeting the 3' untranslated region of the GATA family transcriptional repressor TRPS1 (tricho-rhino-phalangeal syndrome type 1), which inhibited EMT by decreasing ZEB2 (zinc finger E-box-binding homeobox2) expression. (nih.gov)