Loading...
  • substrates
  • Another important aspect of the present work involves the development of pharmacophore models for the new FAE families, for which sufficient information on known substrates existed. (chalmers.se)
  • Conclusions The development of pharmacophore models for specific FAE sub-families will have a huge impact on the application of members of the particular group to completely novel and unexpected substrates. (chalmers.se)
  • synthase
  • Expression cloning of wax synthase cDNAs encoding a member of the acyltransferase enzyme family. (nih.gov)
  • The wax synthase had little or no ability to synthesize cholesteryl esters, diacylglycerols, or triacylglycerols, whereas other acyltransferases, including the acyl-CoA:monoacylglycerol acyltransferase 1 and 2 enzymes and the acyl-CoA:diacylglycerol acyltransferase 1 and 2 enzymes, exhibited modest wax monoester synthesis activities. (nih.gov)
  • The data suggest that wax monoester synthesis in mammals involves a two step biosynthetic pathway catalyzed by fatty acyl-CoA reductase and wax synthase enzymes. (nih.gov)
  • B , expression of mouse and human wax synthase enzyme activities in HEK 293 cells. (nih.gov)
  • Expression vectors encoding nothing ( pCMV , lane 1 ), the human wax synthase enzyme ( hWS , lane 2 ), or the mouse wax synthase enzyme ( mWS , lane 3 ) were introduced into HEK 293 cells by transfection. (nih.gov)
  • Calcium is another special case, in that it is required as a component of the human diet, and it is needed for the full activity of many enzymes, such as nitric oxide synthase, protein phosphatases, and adenylate kinase, but calcium activates these enzymes in allosteric regulation, often binding to these enzymes in a complex with calmodulin. (wikipedia.org)
  • Protein
  • While the amino acid members of the triad are located far from one another on the sequence of the protein, due to folding, they will be very close to one another in the heart of the enzyme. (wikipedia.org)
  • The term coenzyme refers specifically to enzymes and, as such, to the functional properties of a protein. (wikipedia.org)
  • Some consider tightly bound organic molecules as prosthetic groups and not as coenzymes, while others define all non-protein organic molecules needed for enzyme activity as coenzymes, and classify those that are tightly bound as coenzyme prosthetic groups. (wikipedia.org)
  • organisms
  • Background One of the most intriguing groups of enzymes, the feruloyl esterases (FAEs), is ubiquitous in both simple and complex organisms. (chalmers.se)
  • These enzymes all serve the same end function but do so in different organisms without detectable similarity in primary and possibly tertiary structures. (wikipedia.org)
  • Other organisms require additional metals as enzyme cofactors, such as vanadium in the nitrogenase of the nitrogen-fixing bacteria of the genus Azotobacter, tungsten in the aldehyde ferredoxin oxidoreductase of the thermophilic archaean Pyrococcus furiosus, and even cadmium in the carbonic anhydrase from the marine diatom Thalassiosira weissflogii. (wikipedia.org)
  • superoxide
  • The superoxide dismutase enzyme probably gains new, but harmful, properties when altered by SOD1 mutations. (acris-antibodies.com)
  • A popular example of NISE is the Superoxide dismutase family of enzymes which contains three distinct forms (EC 1.15.1.1) Fe,Mn superoxide dismutase Cu,Zn superoxide dismutase Nickel superoxide dismutase CuZn (SOD1) superoxide dismutase was the first to be discovered and is a homodimer containing copper and zinc, found often in intracellular cytoplasmic spaces. (wikipedia.org)
  • function
  • nevertheless, the framework presented here is applicable to every poorly characterized enzyme family and understanding their structure-function relationships. (chalmers.se)
  • Knowledge of the structure and function of these vital enzymes that generate this macromolecule in M. tuberculosis could facilitate the development of potent lead compounds against tuberculosis. (scicombinator.com)
  • However, as the enzyme was conserved in various species over time, the restraints of its function has changed and expanded to meet the biological needs of each organism. (wikipedia.org)
  • Class 3 RNase III Class 3 RNases III include the Drosha family of enzymes known to function in maturation of precursors to miRNA. (wikipedia.org)
  • Class 4 RNase III Class 4 RNases III include the Dicer family of enzymes known to function in RNA interference (RNAi). (wikipedia.org)
  • Mutations in the PLA2G6 gene impair the function of the PLA2 group VI enzyme. (wikipedia.org)
  • This impairment of enzyme function may disrupt cell membrane maintenance and contribute to the development of spheroid bodies in the nerve axons. (wikipedia.org)
  • The particular geometry of the triad members are highly characteristic to their specific function: it was shown that the position of just four points of the triad characterize the function of the containing enzyme. (wikipedia.org)
  • It is an enzyme that catalyzes the breakdown of acetylcholine and of some other choline esters that function as neurotransmitters. (wikipedia.org)
  • Metabolism
  • A number of pyridoxal-dependent enzymes involved in the metabolism of cysteine, homocysteine and methionine have been shown to be evolutionary related. (wikipedia.org)
  • This enzyme participates in selenoamino acid metabolism. (wikipedia.org)
  • undergoes
  • Then, the acyl-enzyme undergoes nucleophilic attack by a water molecule, assisted by the histidine 440 group, liberating acetic acid and regenerating the free enzyme. (wikipedia.org)
  • residues
  • PTU inhibits the enzyme thyroperoxidase, which normally acts in thyroid hormone synthesis by oxidizing the anion iodide (I−) to iodine (I0), facilitating iodine's addition to tyrosine residues on the hormone precursor thyroglobulin. (wikipedia.org)
  • tyrosine
  • The phosphopyridoxyl group is attached to a lysine residue located in the central section of these enzymes and is stabilised by π-stacking interactions with a tyrosine residue above it. (wikipedia.org)
  • It is important to recognize that these enzymes only work on the conjugated tyrosine molecules of T3 and T4: a completely different enzyme family is responsible for the deiodinase activity of iodized single tyrosine molecules within the thyroid follicular cells. (wikipedia.org)
  • roles
  • Collectively, our results reveal a new family of DUBs that may have specialized roles in regulating proteostasis. (sigmaaldrich.com)
  • experimental
  • The underlying functionality of the 12 proposed FAE families was validated against a combination of prediction tools and published experimental data. (chalmers.se)
  • In addition, we provide bioactivities of known M. tuberculosis drugs against these enzymes based on both experimental and computational approaches. (scicombinator.com)
  • activity
  • Interestingly, one CYP716E subfamily enzyme, CYP716E26, exhibited the previously unreported C-6β hydroxylation activity against β-amyrin to produce a rare bioactive triterpenoid, daturadiol (olean-12-ene-3β,6β-diol). (frontiersin.org)
  • Another Popular example of NISE are the Cellulase family of enzymes, particularly Cellulose 1,4-beta-cellobiosidase also consisting of three distinct forms possessing endonuclease activity. (wikipedia.org)
  • In the presence of 0.5mM of Ca(2+), K(+), Na(+), and Zn(2+) the enzyme activity was increased twofold. (wikipedia.org)
  • In the presence of .5 mM of Co(2+) and Mn(2+) the enzyme activity was increased fourfold. (wikipedia.org)
  • Mg(2+) is the ion that increases the enzyme activity the most. (wikipedia.org)
  • With .5 mM of Mg(2+) enzyme activity was increased sixfold. (wikipedia.org)
  • class
  • An international team of scientists have discovered how compounds block flap endonuclease 1 (FEN1) - a crucial enzyme class in the DNA damage response and potential target for cancer treatment. (sheffield.ac.uk)
  • Typically, class I enzymes possess a single RNase III domain (RIIID) followed by a dsRNA-binding domain (dsRBD). (wikipedia.org)
  • eukaryotes
  • In eukaryotes endoV is primarily a ribonuclease and cleaves single-stranded RNA at the 3' position relative to an inosine base, which may be present due to RNA editing by deaminase enzymes. (wikipedia.org)
  • localizes
  • The enzyme encoded by this gene localizes to the endoplasmic reticulum (ER) and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. (wikipedia.org)
  • amino
  • PLP enzymes exist in their resting state as a Schiff base, the aldehyde group of PLP forming a linkage with the epsilon-amino group of an active site lysine residue on the enzyme. (wikipedia.org)
  • The enzyme is made up of 389-441 amino acids and forms four identical subunits. (wikipedia.org)
  • group
  • These enzymes are a group of endoribonucleases that are characterized by their ribonuclease domain, which is labelled the RNase III domain. (wikipedia.org)