• Phosphotransferase systems in Enterococcus faecalis OG1RF enhance anti-stress capacity in vitro and in vivo. (umm.de)
  • To explore a possible role for these antigens in enterococcal infections, the genes encoding these three antigens were disrupted in Enterococcus faecalis OG1RF. (uth.edu)
  • A mouse peritonitis model was used to evaluate the virulence of these mutants and two sets of genes, etaRS and etbRS, were found to be involved in stress response and E. faecalis virulence. (uth.edu)
  • Testing of these mutants in a mouse peritonitis model showed that the LD50s and time courses of killing of mice were similar to that of OG1RF, suggesting that these antigens are not important for the enterococcal infections, at least in this animal model. (uth.edu)
  • To explore a possible role of an E. faecalis gelatinase (encoded by gelE), which belongs to a family of Zn-metalloproteases that have been shown to be virulence factors in other organisms, in enterococcal infections, an insertion mutant was constructed in OG1RF and tested in the mouse peritonitis model. (uth.edu)
  • Testing the virulence of fsr and sprE mutants in the mouse peritonitis model showed that these mutants had significant delays in lethality compared to OG1RF, suggesting that sprE and fsr genes contribute to the virulence of E. faecalis OG1RF in this model. (uth.edu)
  • Enterococcus faecalis and faecium are two major representative clinical strains of the Enterococcus genus and are sadly notorious to be part of the top agents responsible for nosocomial infections. (frontiersin.org)
  • A collection of 19 different phages were used to treat phage-resistant mutant strains of E. faecalis which showed a broad range of host range variability. (biologists.com)
  • The genome showed high similarity with other strains of E. faecalis, including OG1RF, T13, 12107 and T20, based on OrthoANI values. (pacb.com)
  • Genes related to multidrug resistance efflux pumps were annotated in the genome.The comparative genomic analysis of E. faecalis strains presented in this study was performed using a variety of analysis methods and will facilitate future identification of hypothetical proteins. (pacb.com)
  • A similar immunoscreening approach has been used previously in our laboratory to identify potential virulence factors in E. faecalis, and a gene cluster involved in polysaccharide biosynthesis (epa) was identified and shown to be involved in E. faecalis virulence. (uth.edu)
  • To increase our knowledge of E. faecalis virulence factors, we isolated strain CBA7120 from the feces of an 81-year-old female from the Republic of Korea and performed a comparative genomic analysis.The genome sequence of E. faecalis CBA7120 is 3,134,087 bp in length, with a G + C content of 37.35 mol%, and is comprised of four contigs with an N50 value of 2,922,046 bp. (pacb.com)
  • Figure 1: Phylogeny of E. faecalis isolates drawn from across the UK and Ireland. (nature.com)
  • Enterococci are normal flora in the human intestinal tract, and also one of the leading causes of nosocomial infections, with most of the clinical isolates being Enterococcus faecalis and Enterococcus faecium. (uth.edu)
  • Here, using mutation analyses and complementation, we demonstrated that both the ebp (encoding endocarditis and biofilm-associated pili) operon and srtC are important for biofilm production of E. faecalis strain OG1RF. (jci.org)
  • Two sag-like genes (salA and salB) were identified in a strain of Enterococcus faecalis, and the sal gene products had similar N-terminal domains as the Sag proteins, but differed in the C-terminus. (uth.edu)
  • In our initial screening of E. faecalis strain OG1RF genomic libraries, autolysin and a homolog of a protein of Enterococcus faecium previously designated P54 were found to be two major antigens that reacted with human patient sera, and an antigen designated MH-1 antigen that reacted with serum from a endocarditis patient was also identified. (uth.edu)
  • Comprehensive Functional Analysis of the Enterococcus faecalis Core Genome Using an Ordered, Sequence-Defined Collection of Insertional Mutations in Strain OG1RF. (cdc.gov)
  • Enterococcus faecalis, the type strain of the genus Enterococcus, is not only a commensal bacterium in the gastrointestinal tract in vertebrates and invertebrates, but also causes serious disease as an opportunistic pathogen. (pacb.com)
  • Here, using the dRNA-seq technique in combination with ANNOgesic analysis, we successfully mapped and annotated transcription start sites (TSS) of both E. faecalis V583 and E. faecium AUS0004 at single nucleotide resolution. (frontiersin.org)
  • A BLAST search was performed to identify potential two component systems in the E. faecalis V583 genome database using Bacillus subtilis PhoP/PhoS as query sequences. (uth.edu)
  • In the case of the bacteria E. faecalis , a species of enterococci attributed to vancomycin resistance, only the PIP(EF) (Phage infection protein of E. faecalis ) has been identified as a target receptor for phage treatment to date. (biologists.com)
  • As one of my projects, total polysaccharides were extracted from E. faecalis OG1RF and from two epa mutants constructed previously, TX5179 and TX5180, and western blots with patient sera showed that an immuno-reactive polysaccharide present in wild type OG1RF was not produced by either of the two epa mutants. (uth.edu)
  • The epa mutants were more sensitive to ethanol stress, neutrophil killing, and neutrophil phagocytosis than the wild type OG1RF, suggesting that the epa genes are involved in neutrophil phagocytosis and killing of E. faecalis. (uth.edu)
  • Examination of promoter activity in OG1RF and fsr mutants indicated that the fsrB and gelE promoters are fsr dependent and the fsrA promoter is an fsr-independent weak constitutive promoter. (uth.edu)
  • This is most resistance appear to be the dominant contrib- likely a consequence of differences in allele utor to the overall longevity of daf-2 and gens Pseudomonas aeruginosa and Salmonella strengths of the daf-2 and age-1 mutants, all of enterica and the Gram-positive pathogens En- which are partial loss-of-function mutants. (pdftreatment.com)
  • We undertook whole genome sequencing of E. faecalis associated with bloodstream infection in the UK and Ireland over more than a decade to determine the population structure and genetic associations with hospital adaptation. (nature.com)
  • The first whole-genome sequence of E. faecalis was published in 2003 8 . (nature.com)
  • Genome sequence of a commensal bacterium, Enterococcus faecalis CBA7120, isolated from a Korean fecal sample. (pacb.com)
  • Analyzing bacteria in late exponential phase, we capture ~40% ( E. faecalis ) and 43% ( E. faecium ) of the annotated protein-coding genes, determine 5′ and 3′ UTR (untranslated region) length, and detect instances of leaderless mRNAs. (frontiersin.org)
  • Of particular clinical relevance are two rogue enterococcal species, Enterococcus faecalis and Enterococcus faecium ( Van Tyne and Gilmore, 2014 ). (frontiersin.org)
  • This rise was predominantly due to E. faecium , but E. faecalis accounted for 11% of vancomycin-resistant Enterococcus (VRE) bacteraemias in the UK and Ireland (UK&I) between 2001 and 2013 ( http://www.bsacsurv.org ). (nature.com)
  • Macrophage-induced bystander effects have been implicated as an important mediator of chromosomal instability and colon cancer triggered by Enterococcus faecalis , a human intestinal commensal bacteria. (aacrjournals.org)
  • Increasing multidrug resistance in Enterococcus faecalis, a nosocomial opportunist and common cause of bacterial endocarditis, emphasizes the need for alternative therapeutic approaches such as immunotherapy or immunoprophylaxis. (jci.org)
  • Enterococci are one of the leading causes of nosocomial infections, and Enterococcus faecalis causes the majority of enterococcal infections. (uth.edu)
  • These biologically important surface pili, which are antigenic in humans during endocarditis and encoded by a ubiquitous E. faecalis operon, may be a useful immunotarget for studies aimed at prevention and/or treatment of this pathogen. (jci.org)
  • The E. coli clones expressing Sal proteins reacted with sera from patients with E. faecalis endocarditis and the Sal proteins in E. faecalis appeared to be cell-associated. (uth.edu)
  • Northern blot analysis also showed that the fsr genes were highly expressed in post-exponential phase during growth and that the expression of fsr genes were cell-density dependent and could be induced by a secreted inducer(s) in the supernatant of OG1RF cultures. (uth.edu)
  • TNF-α-mediated bystander assays used a murine coculture system of primary colonic epithelial cells and E. faecalis -infected macrophages ( in vitro ), with an interleukin 10 (IL-10)-deficient mouse model of colon cancer that involves long-term colonization with E. faecalis ( in vivo ). (aacrjournals.org)
  • while, in E. faecalis, fsr QS system has been discovered as a homolog of the agr -like QS system (Qin et al. (springeropen.com)
  • In addition, immunogold electron microscopy using antisera against EbpA-EbpC proteins as well as patient serum demonstrated that E. faecalis produces pleomorphic surface pili. (jci.org)
  • The expansion of the repertoire of target phage receptors for E. faecalis, as well as many other bacteria species, forms the cusp of overcoming the ever-emerging dilemma of antibiotic resistance. (biologists.com)
  • Using mother mice infected with both wild-type and EpaS mutant E. faecalis, the extent of transmission of the bacteria from mother to new born mice was tested. (biologists.com)
  • Each of the three main lineages contained a mixture of vancomycin-resistant and -susceptible E. faecalis (VSEfs), which has important implications for infection control and antibiotic stewardship. (nature.com)
  • Extending these observations, colon biopsies from E. faecalis -colonized IL-10 −/− mice exhibited crypt hyperplasia and increased staining for macrophages, TNF-α, netrin-1, NF-κB, Tnfrsf1b, and the proliferation marker proliferating cell nuclear antigen while also displaying a reduction in epithelial cell apoptosis. (aacrjournals.org)
  • Figure 2: Global population structure of E. faecalis . (nature.com)
  • Comprehensive Functional Analysis of the Enterococcus faecalis Core Genome Using an Ordered, Sequence-Defined Collection of Insertional Mutations in Strain OG1RF. (cdc.gov)
  • In this study, we explored the pheromone effect and potential molecular mechanisms of estradiol in promoting the conjugative transfer of pCF10 plasmid in Enterococcus faecalis. (bvsalud.org)
  • BACKGROUND: Enterococcus faecalis (Efa) has been shown to be a "driver bacteria" in the occurrence and development of colorectal cancer (CRC). (bvsalud.org)
  • Enterocin DD14 (EntDD14) is a two-peptide leaderless bacteriocin produced by the Enterococcus faecalis 14 strain previously isolated from meconium. (bvsalud.org)