Facilitate bacterial biofilm formation. Medical search. Frequent questions

Gtfs even bind to the bacteria that do not synthesize Gtfs, and therefore, facilitate interspecies and interkingdom coadhesion. (wikipedia.org)
Biofilm is a complex matrix consisting of extracellular polysaccharides, DNA, and proteins that protect bacteria from a variety of physical, chemical, and biological stresses allowing them to survive in hostile environments. (intechopen.com)
Bacteria can be found in planktonic form or in specific conditions, as sessile aggregates on both biotic and abiotic surfaces originating complex structures known as biofilm. (intechopen.com)
The biofilm matrix consists of polysaccharides, proteins, and DNA and constitutes a stubborn source that protects bacteria from a variety of physical, chemical, and biological stresses. (intechopen.com)
For many bacteria, biofilm formation is a key part of their pathogenic behaviour, and is especially important in the development of medical device-associated infection. (qub.ac.uk)
To facilitate adhesion to surfaces, bacteria can express carbohydrate-binding proteins, known as lectins. (qub.ac.uk)
Biofilms are conglomerations of bacteria (or certain fungi) within a 'slime' of polysaccharides, proteins and DNA. (ivis.org)
This extracellular matrix assists bacteria in adhering to surfaces and can be formed by some bacterial strains when they change from a free-living 'planktonic' form to a sessile form for example on the surfaces of teeth, heart valves, lungs but also on implants, in skin wounds or when floating on moist surfaces such as sewage or even disinfectant footbaths. (ivis.org)
In nature, biofilms are ubiquitous and although biofilm-associated bacteria grow slower than their planktonic counterparts, biofilms help bacteria to thrive in difficult or even extreme conditions such as hot springs or on glaciers. (ivis.org)
Free-swimming bacteria settle on a surface to cooperate and form a protective biofilm. (the-scientist.com)
Cariogenic bacteria are found in the complex biofilm which contains many kinds of bacteria called dental plaque. (positivehealth.com)
Due to the resistance of some bacteria to antibiotics, research in the field of dealing with bacterial infections is necessary. (nature.com)
Bacteria can enhance their survival by attaching to inanimate surfaces or tissues, and presenting as multicellular communities encased in a protective extracellular matrix called biofilm. (akjournals.com)
More like a sooty dust than an ooze, biofilms are entire ecosystems of bacteria, algae, and fungi all living together in a funky and super-stubborn veneer. (nationalgeographic.com)
The bacteria produce protective pigments, and the gel itself may facilitate darkening by collecting particles from the air. (nationalgeographic.com)
Contamination of dairy products is mostly due to bacteria being released from biofilm in the processing plant rather from the farm itself. (afpm.org.my)
Flagella-mediated motility is also important for many pathogens - including Salmonella enterica - and allows the bacteria to reach the site of infection, facilitate host-pathogen interactions, and promote biofilm formation. (hu-berlin.de)
The bacterial flagellum is by far the most prominent extracellular structure known in bacteria and made through self-assembly of several tens of thousand individual building blocks. (hu-berlin.de)
Bacteria often switch from a free-living lifestyle to a surface adapted, structured lifestyle known as a biofilm. (southampton.ac.uk)
We found that subpopulations of biofilm bacteria undergo cell death and lysis as a feature of the Pseudomonas aeruginosa biofilm life cycle. (southampton.ac.uk)
We isolated and named this phage, showed that it becomes superinfective to its host during biofilm development, and found that it plays a role in phenotypic diversification of biofilm bacteria. (southampton.ac.uk)
Antimicrobials work by delaying or killing bacteria by targeting important functions of bacteria, such as translation, transcription, cell wall formation, and deoxyribonucleic acid (DNA) replication. (amis-childrenshome.org)
Antisera-based methods have also been demonstrated to enhance deoxyribonuclease-induced biofilm destruction and antibiotic killing, as well as boost macrophages' ability to fight bacteria. (amis-childrenshome.org)
In bacteria and archaea , structural proteins with related functions are usually encoded together within the genome in a block called an operon and are transcribed together under the control of a single promoter , resulting in the formation of a polycistronic transcript ( Figure 12.30 ). (pressbooks.pub)
Both bacteria were also able to utilize MUC5B as a sole nutrient source during early biofilm formation, individually and synergistically in a dual species biofilm. (diva-portal.org)
Therefore, biofilm matrix gives the additional resistance power to bacteria which makes them to not only tolerate harsh conditions but also resistant to antibiotics which lead to the emergence of bad bugs infections like multi drug resistant, extensively drug resistant and totally drug resistant bacteria. (biomedcentral.com)
Key to this ability is bacterial motility, which allows bacteria to move away from unfavourable conditions and to move towards favourable conditions. (uni-marburg.de)
Part 2: Transcriptional regulation of biofilms is mediated by RemA, which interacts with DNA in a histone-like manner Instead of a motile lifestyle, bacteria can also establish a multicellular, sessile lifestyle in the form of biofilms. (uni-marburg.de)
Type IV pili are ubiquitous surface appendages in Gram-negative bacteria that promote diverse activities including attachment, virulence, biofilm formation, horizontal gene transfer, and twitching motility [ 1 - 5 ]. (biorxiv.org)
Chaperone-Usher Pathway (CUP) pili are major adhesins in Gram-negative bacteria, mediating bacterial adherence to biotic and abiotic surfaces. (cam.ac.uk)
Bacteria predominantly grow inside multicellular communities attached to solid surfaces and enclosed in a self-produced polymeric matrix, called biofilms [ 1 ]. (biomedcentral.com)
Because of the protected environment, bacteria within biofilms are less sensitive to environmental stresses, including disinfectants and antibiotics, and are as a consequence very difficult to eradicate [ 3 ]. (biomedcentral.com)
[13] The biofilm bacteria can share nutrients and are sheltered from harmful factors in the environment, such as desiccation, antibiotics, and a host body's immune system. (wikipedia.org)
They can be found very early in Earth's fossil records (about 3.25 billion years ago) as both Archaea and Bacteria, and commonly protect prokaryotic cells by providing them with homeostasis, encouraging the development of complex interactions between the cells in the biofilm. (wikipedia.org)
[8] [5] The first colonist bacteria of a biofilm may adhere to the surface initially by the weak van der Waals forces and hydrophobic effects. (wikipedia.org)
In the first few years of life, the bacterial microbiota within the mouth is predominantly aerobic, but, as the teeth develop, favorable sites supporting pathogenic anaerobic bacteria emerge. (medscape.com)
Most bacterial nosocomial pneumonias occur by aspiration of bacteria colonizing the oropharynx or upper gastrointestinal tract of the patient. (cdc.gov)

Any drug impairing crucial processes for bacterial life will inevitably lead to the development of drug-resistant strains, whereas the inhibition of biofilm formation might prevent the onset of bacterial resistance. (intechopen.com)
In this section, we will focus on proteins involved in biofilm formation as useful targets for the development of new drugs that can effectively and specifically impair biofilm formation with slight effects on cell survival, thus avoiding the generation of drug-resistant strains. (intechopen.com)
To evaluate antibacterial behavior, we used two bacterial strains Gram-negative Escherichia coli ( E. coli ) and Gram-positive Staphylococcus aureus ( S. aureus ). (nature.com)
The Ti-Cu films displayed superior antibacterial ability, with a 99.9% reduction (equivalent to a 5-log reduction) in bacterial viability after 2 h compared to Ti alone against both bacterial strains. (nature.com)
However, excessive and indiscriminate use of antibiotics has resulted in the development of antibiotic resistance among bacterial strains 14 . (nature.com)
The strains were allowed to form biofilm in vitro. (jmb.or.kr)
The aims of this study were to investigate the biofilm formation and the relation of this property with genotype and antibiotic susceptibilities of P.aeruginosa strains isolated from CF patients. (omu.edu.tr)
Most of the biofilm positive strains belonged to K1 (n= 5) and K2 (n= 6) genotypes while biofilm negative isolates were in the L (n= 8) and O (n= 7) genotypes. (omu.edu.tr)
We showed that strains of Pseudomonas aeruginosa with increased mutation rates (caused by deletion of genes involved in DNA mismatch repair) produced more biofilm biomass in laboratory experiments. (southampton.ac.uk)
We also engineered SigL overexpressing strains and observed enhanced biofilm formation in the CDC1 background, and reduced spore titers as well as dampened sporulation kinetics in both strains. (arizona.edu)
Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)

An increase in bacterial mutation rate can promote biofilm formation. (southampton.ac.uk)
While classical CUP pili have been extensively characterized, little is known about so-called archaic CUP pili, which are phylogenetically widespread and promote biofilm formation by several human pathogens. (cam.ac.uk)

The inhibition of quorum sensing, called quorum quenching, may disrupt oral biofilm formation, which is the main etiological factor of oral diseases, including periodontitis. (mdpi.com)
Cells may also communicate via quorum sensing, which may in turn affect biofilm processes such as detachment. (cdc.gov)
We study with it biofilm formation, quorum sensing in prokaryotes and infection biology, fungal growth as well as immune cell responses in fungal interactions. (uni-wuerzburg.de)
Quorum sensing is a type of bacterial communication that occurs via the secretion of autoinducer molecules (5). (kenyon.edu)
Quorum sensing plays an important role in regulating the biofilm formation. (biomedcentral.com)
The role of quorum sensing in facilitating the communication processes in biofilms in magnificent. (edu.in)

In summary, we demonstrated that P. aeuginosa clinical isolates with novel epigenetic markers could form excessive biofilm, which might enhance its antibiotic resistance and in vivo colonization in COVID-19 patients. (frontiersin.org)

For example, in Bacillus subtilis, Mycobacterium smegmatis, and Pseudomonas aeruginosa biofilms, calcite (CaCO3) contributes to the integrity of the matrix. (wikipedia.org)
Pseudomonas aeruginosa is a biofilm-forming opportunistic pathogen which causes chronic infections in immunocompromised patients and leads to high mortality rate. (frontiersin.org)
Role of a new filamentous prophage Pf4 in Pseudomonas aeruginosa biofilm development. (southampton.ac.uk)
This project will exploit novel nitric oxide based therapies to improve the effectiveness of antibiotics against Pseudomonas aeruginosa biofilms. (southampton.ac.uk)
Antisera against DNABII proteins like the integration host factor A (IhfA) can disrupt biofilms generated by the ESKAPE pathogenic organisms ( Enterococcus faecium , Staphylococcus aureus species , Klebsiella pneumoniae , Acinetobacter baumannii , Pseudomonas aeruginosa , and Enterobacter species). (amis-childrenshome.org)
Architecture of the biofilm-associated archaic Chaperone-Usher pilus CupE from Pseudomonas aeruginosa. (cam.ac.uk)
Bacterial metabolism selected lignin model compounds (vanillin, vanillate, 4- hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a Introduction culture collection. (lu.se)

Studies on P. aeruginosa, B. subtilis, V. cholerae, and S. mutans suggested that the transition from initial cell clustering to microcolony appears to be conserved among different biofilm-forming model organisms. (wikipedia.org)
We collected a strong biofilm-forming P. aeruginosa strain displaying small colony variant morphology from a severe COVID-19 patient. (frontiersin.org)
For P. aeruginosa , low doses of NEBB significantly reduced biofilm mass and metabolic activity while higher doses of NEBB increased biofilm mass and metabolic activity. (jmb.or.kr)
Biofilm production was found positive in 33.3% (20/60) of P.aeruginosa tested. (omu.edu.tr)
The data obtained from this study indicated that biofilm negative and positive P.aeruginosa isolates clustered in different groups. (omu.edu.tr)
Further work has shown the importance of this phage in the P. aeruginosa biofilm lifecycle and virulence. (southampton.ac.uk)
Imaging CupE pili on the surface of P. aeruginosa cells using electron cryotomography shows that CupE pili adopt variable curvatures in response to their environment, which might facilitate their role in promoting cellular attachment. (cam.ac.uk)
Finally, bioinformatic analysis shows the widespread abundance of cupE genes in isolates of P. aeruginosa and the co-occurrence of cupE with other cup clusters, suggesting interdependence of cup pili in regulating bacterial adherence within biofilms. (cam.ac.uk)
Taken together, our study provides insights into the architecture of archaic CUP pili, providing a structural basis for understanding their role in promoting cellular adhesion and biofilm formation in P. aeruginosa. (cam.ac.uk)

However, some patients may have bacterial infection despite negative urine cultures. (medscape.com)
The impact of biofilms on microbial infection stems from their ability to protect pathogens from antimicrobial drugs and disinfectants (intrinsic resistance), to help to retain nutrients and to protect microbes from host immune responses (antibodies can penetrate biofilms and phagocytes engulf them but the resulting immune reactions are reduced). (ivis.org)
Detachment and expulsion of aggregates of biofilm allows the spread of infection and may facilitate thromboembolic disease. (ivis.org)
Methods of inhibition of FimH activity in a human subject are also provided for the treatment of diseases such as bacterial infection, Crohn's disease (CD) and Inflammatory Bowel Disease (IBD). (justia.com)
This tolerance often links biofilms with persistent and chronic infection, and provides ideal conditions for the acquisition or evolution of AMR. (southampton.ac.uk)
However, considering the type and location of the infection, their potential ability to send planktonic cells and aggregates into the microenvironment, facilitating bacterial dissemination to different sites of infection or triggering bacteremia, should be considered. (amis-childrenshome.org)
HAP is a common nosocomial bacterial infection and is most prevalent in medical and surgical intensive care units (ICUs). (medscape.com)
Apical periodontal infection has been associated with 200 bacterial species, and 500 bacterial species have been reported with marginal periodontitis. (medscape.com)
[ 3 , 4 ] Evidence also exists that significant interaction of bacterial types within biofilm may either enhance or suppress metabolic activity that leads to dental infection. (medscape.com)
Adherence and the slow-growing nature of Candida facilitate its ability to colonize and disseminate into the bloodstream and body tissues before clinical signs and symptoms of infection become apparent. (medscape.com)

It is envisaged that these dual-functional materials will be capable of inhibiting biofilm formation when deployed as a coating on medical devices, protecting patients from bacterial infections, while simultaneously providing visual feedback to healthcare professionals if the material has reached the end of its anti-infective lifespan. (qub.ac.uk)
It is a biofilm-forming opportunistic pathogen causing life-threatening chronic infections in immunocompromised individuals with diseases like burn wounds, urinary tract infections, and respiratory infections ( Gellatly and Hancock, 2013 ). (frontiersin.org)
However, over the past 20 years, biofilms have become recognized as a serious problem for public health as they facilitate many important microbial infections in humans and animals, including wound and implant-related infections, and substantially complicate their treatment. (ivis.org)
Many of these infections are initiated by a critical interaction between host ligands (frequently polysaccharide moieties) and bacterial adhesins (frequently expressed at the distal tip of polymeric pilus fibers assembled by the chaperone-usher pathway). (justia.com)
Biofilms have great importance for public health because of their role in certain infectious diseases and importance in a variety of device-related infections. (cdc.gov)
Bacterial infections pose a significant threat to human health and cause various diseases. (nature.com)
Over the years, antibiotics have played a crucial role in combating bacterial infections by benefiting from technological advancements and medical knowledge 13 . (nature.com)
There are many approaches being used to control infections by suppressing its formation but CRISPR-CAS (gene editing technique) and photo dynamic therapy (PDT) are proposed to be used as therapeutic approaches to subside bacterial biofim infections, especially caused by deadly drug resistant bad bugs. (biomedcentral.com)
In fact, it was shown that the majority of all bacterial infections are related to biofilm growth, stressing the importance of this life style [ 2 ]. (biomedcentral.com)
The formation of bacterial biofilms is responsible for hospital-acquired (nosocomial) infections. (pasteur.fr)
Sections on the prevention of bacterial pneumonia in mechanically ventilated and/or critically ill patients, care of respiratory-therapy devices, prevention of cross-contamination, and prevention of viral lower respiratory tract infections (e.g., respiratory syncytial virus {RSV} and influenza infections) have been expanded and updated. (cdc.gov)

A practical approach utilized in this study involves the preparation of an antibacterial thin film on the surfaces, which can effectively inhibit and reduce biofilm formation and bacterial adherence. (nature.com)
These findings highlight that adding Cu to Ti can improve the antibacterial ability of the surface while inhibiting bacterial adherence. (nature.com)
The role of cell-surface properties was investigated using hydrophobicity (bacterial adherence to hydrocarbons), autoaggregation and coaggregation assays. (biomedcentral.com)
Both isolates displayed a hydrophilic cell surface following the bacterial adherence to xylene assay. (biomedcentral.com)
In biofilms, extracellular deoxyribonucleic acid (eDNA) serves as a structural template within the extracellular matrix and could control aggregation and adherence to host tissues. (amis-childrenshome.org)
DNA-binding proteins are essential for the biofilm matrix's adherence, structuring, and stability. (amis-childrenshome.org)
Bacterial type IV pili are critical for diverse biological processes including horizontal gene transfer, surface sensing, biofilm formation, adherence, motility, and virulence. (biorxiv.org)
The researchers speculated that the yeast forms are critically important for adherence and tissue dissemination and that hyphal formation in the tissues contributes to parenchymal destruction. (medscape.com)

These compounds are important in biofilm formation and cells' attachment to surfaces. (wikipedia.org)
The initial bacterial adhesion to surfaces involves the adhesin-receptor interactions. (wikipedia.org)
First, the EPS formation takes place at the site of adhesion, it will be either produced on bacterial surfaces or secreted on the surface of attachment, and form an initial polymeric matrix promoting microbial colonization and cell clustering. (wikipedia.org)
This project aims to develop new carbohydrate-functionalised polymeric surfaces which will inhibit bacterial adhesion. (qub.ac.uk)
Microorganisms attach to surfaces and develop biofilms. (cdc.gov)
5) in 1978 put forth a the- on exposed surfaces led to studies that revealed surface-associ- ory of biofilms that explained the mechanisms whereby micro- ated microorganisms (biofilms) exhibited a distinct phenotype organisms adhere to living and nonliving materials and the with respect to gene transcription and growth rate. (cdc.gov)
Van Leeuwenhoek, using his simple microscopes, first utilization of the confocal laser scanning microscope to char- observed microorganisms on tooth surfaces and can be cred- acterize biofilm ultrastructure, and an investigation of the ited with the discovery of microbial biofilms. (cdc.gov)
Biofilms may form on a wide variety of surfaces, isms (based on cell morphology). (cdc.gov)
The ability of E. meningoseptica isolates to adhere to abiotic surfaces and form biofilm structures may result from the hydrophilic cell surface and multiple adhesins located around the cell. (biomedcentral.com)
But the white surfaces of the newer American monuments are a clean slate for observing how biofilms develop, grow, and spread and so are a valuable experimental research ground for helping clarify how biofilms may be damaging-or protecting-the world's great historic stone monuments. (nationalgeographic.com)
It was shown for example that an incomplete LPS fraction does not affect biofilm formation capacity of Salmonella on hydrophobic gallstone surfaces, but highly reduces its biofilm capacity on hydrophilic glass surfaces [ 7 ]. (biomedcentral.com)
Biofilms may form on living (biotic) or non-living (abiotic) surfaces and can be prevalent in natural, industrial, and hospital settings. (wikipedia.org)
Evidence of microbial mats include wrinkles, pustular textures, and possible microbial mat chips on the bedding surfaces as well as a matrix fabric that contains possible microbial sheaths and bacterial spherules. (mapress.com)

Metabolic activity of the bacterial biofilm communities have altered as compared to the planktonic one such as increased rates of EPS production, activation or inhibition of particular genes associated with biofilm formation and decreased growth rate [ 39 ]. (biomedcentral.com)
Unlike antibacterials that aim to inhibit cell growth, antivirulence therapies are based on the inhibition of bacterial virulence. (nature.com)

Imke's paper on how the hook length of the bacterial flagellum is optimized for maximal maximal motility performance has been published in PLoS Biology ! (hu-berlin.de)
Bacterial motility is largely enabled by flagella. (uni-marburg.de)
This regulator itself shows an enhanced expression in the presence of high c-di-GMP concentrations [ 12 ], a secondary messenger molecule which has been studied extensively in regulation of bacterial multicellular behavior, motility and virulence [ 13 ]. (biomedcentral.com)
Subpopulations of cells within the biofilm differentiate to perform various activities for motility, matrix production, and sporulation, supporting the overall success of the biofilm. (wikipedia.org)

NGAs attack bacterial virulence factors to destroy pathogenic potential while preserving bacterial survival. (amis-childrenshome.org)
Pathogenic bacterial biofilms can be life threatening, tremendously decrease affected individual's prime quality of life and are a substantial burden on the healthcare system. (cellexusinc.com)

Clinical isolates of A. baumanii were used to generate a biofilm layer on the endotracheal tube. (rcjournal.com)
While 9 of these 20 isolates were of mucoid colony morphotype, among the 40 biofilm negative isolates mucoid colony was detected in 16 of them. (omu.edu.tr)
Nine of these genotypes composed of biofilm positive isolates and 15 were biofilm negative ones. (omu.edu.tr)
E. meningoseptica isolates CH2B and NCTC 10016 T demonstrated stronger biofilm formation in nutrient-rich medium compared to nutrient-poor medium at both 21 and 37°C, respectively. (biomedcentral.com)
Thirty-three B. licheniformis isolates varied in their ability to form biofilm on stainless steel with stronger biofilm in the presence of minerals. (afpm.org.my)
Paper III examines the regulatory roles of salivary MUC5B on biofilm attachment and metabolic output in two clinical isolates of oral commensals, S. gordonii CW and Actinomyces naeslundii CW. (diva-portal.org)

Differential gene expression analysis indicated that this isolate formed excessive biofilm by reducing flagellar formation (7.4 to 1,624.1 folds) and overproducing extracellular matrix components including CdrA (4.4 folds), alginate (5.2 to 29.1 folds) and Pel (4.8-5.5 folds). (frontiersin.org)

or most of the history of microbiology, microorganisms in these biofilms was polysaccharide. (cdc.gov)
Since that time, the film microorganisms have been shown to elicit specific mech- studies of biofilms in industrial and ecologic settings and in anisms for initial attachment to a surface, development of a environments more relevant for public health have basically community structure and ecosystem, and detachment. (cdc.gov)
The strategy we're looking at now is not to kill these microorganisms but to disarm them," says Federica Villa, a microbiologist at the University of Milan who has been studying D.C.'s biofilms with Montana State University's Center for Biofilm Engineering . (nationalgeographic.com)
A biofilm comprises any syntrophic consortium of microorganisms in which cells stick to each other and often also to a surface. (wikipedia.org)
Biofilms can attach to a surface such as a tooth or rock, and may include a single species or a diverse group of microorganisms. (wikipedia.org)
The formation of a biofilm begins with the attachment of free-floating microorganisms to a surface. (wikipedia.org)
Biofilms are the consortium of microorganisms encased within the slimy sheath of Extracellular Polymeric Substance. (edu.in)
The microorganisms inhabiting the biofilms display a stratified structure, in which the role of every species is fixed and pre-determined as per the environmental suitability and synchronization. (edu.in)

They provide structural integrity to biofilm matrix and act as a scaffold to protect bacterial cells from shear forces and antimicrobial chemicals. (wikipedia.org)
Biofilm cultures were treated with NEBB containing enzymes targeted at lipids, proteins, and sugars, also containing the mucolytic compound N-acetyl cysteine, along with antimicrobial extracts from cranberry, berberine, rosemary, and peppermint. (jmb.or.kr)
Biofilms are notorious for their resistance to environmental stresses, including antimicrobial compounds. (southampton.ac.uk)
Treatment of biofilms with nitric oxide also reduced their resistance to antimicrobial compounds. (southampton.ac.uk)
Extracellular enzymes such as the sporulation-specific extracellular nuclease (NucB), pyocyanin demethylase (PodA), and deoxyribonuclease I (DNase I) cause forced cellular dispersal from biofilms into the environment in an antimicrobial-sensitive planktonic form. (amis-childrenshome.org)

Treatment of established biofilm with NEBB resulted in biofilm-disruption, involving significant reductions in biofilm mass and metabolic activity for Candida and both Staphylococcus species. (jmb.or.kr)

Biofilm formation requires three different stages: cell attachment to a solid substrate, adhesion, and growth. (intechopen.com)
This mucoid structure contains a dense amount of alginate type polysaccharide which facilitates bacterial attachment to lung epithelia and provides protection from the immune system due to biofilm formation. (omu.edu.tr)
S. gordonii facilitated adhesion of A. naeslundii to MUC5B during early attachment. (diva-portal.org)
Glycopeptidolipids are indispensable for initial surface attachment during biofilm formation in Mycobacterium smegmatis [ 111 ]. (biomedcentral.com)
Microbes form a biofilm in response to a number of different factors, [9] which may include cellular recognition of specific or non-specific attachment sites on a surface, nutritional cues, or in some cases, by exposure of planktonic cells to sub-inhibitory concentrations of antibiotics . (wikipedia.org)

V. cholerae also forms biofilms upon colonization on their copepod symbionts. (kenyon.edu)
The specific responses elicited by MUC5B in paper II-III seem to promote commensal colonization while down-regulating dysbiosis-related biofilm activities. (diva-portal.org)

Biofilms are an ensemble of microbial cells irreversibly associated with a surface and enclosed in an essentially self-produced matrix. (intechopen.com)
An estab- lished biofilm structure comprises microbial cells and EPS, has a defined architecture, and provides an optimal environment for the exchange of genetic material between cells. (cdc.gov)
A biofilm is an assemblage of microbial cells that is irre- organisms could attach. (cdc.gov)
The microbial cells growing in a biofilm are physiologically distinct from planktonic cells of the same organism, which, by contrast, are single cells that may float or swim in a liquid medium. (wikipedia.org)

abstract = "The alternative sigma factor SigL (Sigma-54) facilitates bacterial adaptation to the extracellular environment by modulating the expression of defined gene subsets. (arizona.edu)

Thoma and Goldman 14 affirmed that secondary cementum deposition occurs through four different processes: tooth continuous eruption, presence of inflammatory reaction, formation of spicules, and root repair. (bvsalud.org)
A greater understanding of biofilm processes should lead to novel, effective control strategies for biofilm control and a resulting improvement in patient management. (cdc.gov)
The identified phosphoproteins were involved in various bacterial processes, several associated to dysbiosis and development of biofilm-induced disease. (diva-portal.org)
Furthermore, RemA is implicated in processes which enable a cellular protection against osmotic pressure, which occurs during biofilm formation. (uni-marburg.de)
Taken together, this work represents an important step towards the understanding of the transcriptional processes that govern biofilm-formation and osmoprotection in Bacillus subtilis. (uni-marburg.de)
Knowledge about the complex regulatory processes in biofilm formation can provide more insight into survival strategies of S. Typhimurium in non-host environments and can be the fundament of new eradication methods. (biomedcentral.com)

Microbial forms living in biofilms are morphologically and functionally distinct from those of free-floating (planktonic) forms of the same species. (jmb.or.kr)
Growth pattern of Mycobacterium species are different such as M. chelonae and M. fortuitum form biofilm as vertical and entire surface growth, respectively [ 93 ]. (biomedcentral.com)
Author Summary Almost all bacterial species use thin surface appendages called pili to interact with their environments. (biorxiv.org)
Ralstonia solanacearum species complex (RSSC) is a soil borne plant pathogen causing bacterial wilt on various important crops, including Solanaceae plants. (ppjonline.org)

The bacterial pathogens within the RSSC produce exopolysaccharide (EPS), a highly complicated nitrogen-containing heteropolymeric polysaccharide, as a major virulence factor. (ppjonline.org)

Regulations in protein expression between the different growth conditions provides insights in bacterial mechanisms for adaptation to the biofilm lifestyle. (diva-portal.org)
In the future, the structural investigation of RemA-DNA-interaction is facilitated by the insights obtained in the context of this work. (uni-marburg.de)

The results suggest that targeted nutraceutical support may help disrupt biofilm communities, offering new facets for integrative combinational treatment strategies. (jmb.or.kr)

Type 1 pili are anchored in the bacterial outer membrane and are largely composed of repeating FimA protein subunits which form a helically wound cylinder that comprises the thick pilus rod. (justia.com)
Hypercementosis, so-called cementum hyperplasia, constitutes the excessive formation of this tissue and comprises either a limited point or the entire root surface 13 . (bvsalud.org)

Targeting these proteins with multivalent glycosides can inhibit biofilm-formation by crosslinking these lectins. (qub.ac.uk)
It has been demonstrated that directing the immunological system toward biofilm-associated proteins severely disrupts the structural integrity of extracellular DNA and the biofilm. (amis-childrenshome.org)
[2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)

Biofilms are home to millions of microbes, but disrupting their interactions could produce more effective antibiotics. (the-scientist.com)

Afterwards, as biofilm becomes established, EPS provides physical stability and resistance to mechanical removal, antimicrobials, and host immunity. (wikipedia.org)
The microenvironment within biofilms includes signaling channels that facilitate bacterial communication and possibly exchange of resistance genes (acquired resistance) and lead to a change in gene expression and production of virulence factors that are not typically expressed by planktonic forms. (ivis.org)
In biofilms, bacterial cells establish a division of labour and establish an increased resistance against antibiotics and environmental hazardous conditions. (uni-marburg.de)

If you know the mechanisms behind the formation of biofilms, you can work with more elegant solutions instead of using, or abusing, biocides or antibiotics. (nationalgeographic.com)

This can contribute to biofilm dispersal, and provides a mechanism for the release of extracellular DNA (eDNA) within biofilms. (southampton.ac.uk)

3) used scanning and transmission electron microscopy to ated organisms also differ from their planktonic (freely examine biofilms on trickling filters in a wastewater treatment suspended) counterparts with respect to the genes that are tran- plant and showed them to be composed of a variety of organ- scribed. (cdc.gov)
As abundant and omnipresent organisms, bacterial biofilms can form on any surface, at any time. (nationalgeographic.com)

Because intubation and mechanical ventilation alter first-line patient defenses, they greatly increase the risk for nosocomial bacterial pneumonia. (cdc.gov)

Biofilm-associated cells can be differentiated from their suspended counterparts by generation of an extracellular polymeric substance (EPS) matrix, reduced growth rates, and the up- and down-regulation of specific genes. (cdc.gov)
Two major thrusts in the last decade cells that is enclosed in an extracellular polymeric substance have dramatically impacted our understanding of biofilms: the matrix. (cdc.gov)

Nitric oxide-mediated regulation of biofilm dispersal. (southampton.ac.uk)
We discovered that exogenous nitric oxide can act as a signal for the dispersal of bacterial biofilms. (southampton.ac.uk)

[10] [11] A cell that switches to the biofilm mode of growth undergoes a phenotypic shift in behavior in which large suites of genes are differentially regulated . (wikipedia.org)

Global virulence regulation pathways can be targeted to construct NGAs and battle many virulence factors simultaneously without directly affecting bacterial growth. (amis-childrenshome.org)

For B. burgdorferi , we observed reduced biofilm mass, but the remaining residual biofilm showed a mild increase in metabolic activity, suggesting a shift from metabolically quiescent, treatment-resistant persister forms of B. burgdorferi to a more active form, potentially more recognizable by the host immune system. (jmb.or.kr)
Microbial biofilms form on liquid/solid interfaces in nature, such as rocks and clay particles and decaying plant materials. (jmb.or.kr)
Additional in vitro and in vivo data show that PilT and PilU act as independent homo-hexamers that may form a complex to facilitate pilus retraction. (biorxiv.org)
[8] Biofilms can form on the teeth of most animals as dental plaque , where they may cause tooth decay and gum disease . (wikipedia.org)
A biofilm usually begins to form when a free-swimming bacterium attaches to a surface. (wikipedia.org)
We report here that myricetin, but not its glycosylated form, can remarkably decrease the production of several S. aureus virulence factors, including adhesion, biofilm formation, hemolysis and staphyloxanthin production, without interfering with growth. (nature.com)

To carry out their function, pili dynamically extend and retract from the bacterial surface. (biorxiv.org)

A class of biocides known as quaternary ammonium compounds, or quats, is one chemical treatment that temporarily diminishes biofilms and their visible effects. (nationalgeographic.com)
My research group aims to understand how biofilms develop and disperse and how they respond to environmental stresses including therapeutic compounds. (southampton.ac.uk)

Field emission scanning electron microscopy (FE-SEM) images verified that both E. coli and S. aureus cells were physically deformed and damaged the bacterial cell ultrastructure was observed. (nature.com)

Chronic bacterial prostatitis (CBP) is most often caused by Escherichia coli or other gram-negative Enterobacteriaceae, and typically affects men 36 to 50 years of age. (medscape.com)

Thus, both in bacterial (B.subtilis, S.aureus) and in fungal systems ( C. albicans , A. fumigatu s) it proofed useful to model dynamic network responses. (uni-wuerzburg.de)

The only pre-requisite for optimising such things is that the complete microbial profile, architecture and kinetics of biofilms should be known. (edu.in)

EPSs are the construction material of bacterial settlements and either remain attached to the cell's outer surface, or are secreted into its growth medium. (wikipedia.org)
METHODS: We first detected the effect of palbociclib on cell survival, growth and cycle distribution separately by MTT, colony formation and flow cytometry. (bvsalud.org)
Ironically, the stepwise enactment since the 1970s of the Clean Air Act may have reduced the acidity of rainwater to an extent that it no longer hinders the growth of these bacterial communities. (nationalgeographic.com)
Three different reconstituted WPC media at 1%, 5%, and 20% were used for biofilm growth and our results showed that B. licheniformis formed the best biofilm at 1% (low solids). (afpm.org.my)
tryptic soy broth, 1% reconstituted WPC80, and 1% reconstituted WPC80 enriched with lactose and minerals to examine biofilm growth of B. licheniformis on stainless steel. (afpm.org.my)
Biofilms are increasingly recognised as the predominant mode of bacterial growth including within medical, engineered, and environmental contexts. (southampton.ac.uk)
20 generations, a few days of biofilm growth) within biofilms. (southampton.ac.uk)
Paper II studies differences between the S. gordonii DL1 general proteomes in planktonic and biofilm growth phases, and the regulatory effects of salivary mucin MUC5B on protein expression in the biofilm cells. (diva-portal.org)

E. meningoseptica isolate CH2B and strain NCTC 10016 T displayed a strong biofilm-forming phenotype which may play a role in its potential pathogenicity in both clinical and aquaculture environments. (biomedcentral.com)

Heukelekian and genes involved in cell adhesion and biofilm formation. (cdc.gov)

Biofilm architecture of isolate CH2B alone as well as in co-culture with Listeria monocytogenes was investigated using flow cells and microscopy. (biomedcentral.com)

Next, continuous production of EPS further expands the matrix in 3 dimensions while forming a core of bacterial cells. (wikipedia.org)
osmium tetroxide fixative, these researchers were also able to The variable nature of biofilms can be illustrated from scan- show that the matrix material surrounding and enclosing cells ning electron micrographs of biofilms from an industrial water system and a medical device, respectively (Figures 1 and 2). (cdc.gov)
Extracellular polysaccharides secreted by cells are important matrix constituents, contributing to biofilm formation and persistence. (amis-childrenshome.org)
We monitor and predict cooperative pathway changes in B. subtilis biofilm differentiation and oncolytic vaccinia virus acting on cancer cells applying Boolean semi-quantitative simulations. (uni-wuerzburg.de)
Similarly, all cells in two pure bacterial cultures inoculated from the same starting colony contain the same DNA, with the exception of changes that arise from spontaneous mutations. (pressbooks.pub)
Similarly, how is it that the same bacterial cells within two pure cultures exposed to different environmental conditions can exhibit different phenotypes? (pressbooks.pub)
Individual bacterial and yeast cells often produce molecules that are used by others. (elifesciences.org)

The biofilm can then disperse, leading to a burst of infectivity. (kenyon.edu)

After an episode of acute bacterial prostatitis, approximately 5% of patients may progress to CBP. (medscape.com)

iii) colony formation and biofilm maturation. (biomedcentral.com)

The post-treatment biofilm mass was evaluated by crystal-violet staining, and metabolic activity was measured using the MTT assay. (jmb.or.kr)
Average biofilm mass and metabolic activity for NEBB-treated biofilms were compared to the average of untreated control cultures. (jmb.or.kr)

EPSs establish the functional and structural integrity of biofilms, and are considered the fundamental component that determines the physicochemical properties of a biofilm. (wikipedia.org)
Developing NGAs that undermine the structural configuration of bacterial biofilms could be a potential method. (amis-childrenshome.org)

Anatomy1

Organisms9

Chemicals and Drugs9

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes9

Technology, Industry, Agriculture2

Health Care1

Bacteria37

Strains11

Promote biofilm formation2

Quorum6

Antibiotic resistance1

Pseudomonas7

Aeruginosa9

Infection10

Infections11

Adherence8

Surfaces13

Inhibition2

Motility4

Pathogenic2

Isolates6

Extracellular matrix1

Microorganisms8

Antimicrobial5

Staphylococcus1

Attachment5

Colonization2

Microbial cells4

Abstract1

Processes6

Species4

Polysaccharide1

Insights2

Disrupt1

Comprises2

Proteins3

Microbes1

Resistance3

Antibiotics1

Release of extracellular1

Organisms2

Nosocomial1

Polymeric2

Dispersal2

Phenotypic1

Regulation1

Form6

Pili1

Compounds2

Ultrastructure1

Chronic1

Aureus1

Kinetics1

Growth8

Phenotype1

Genes involved1

Isolate1

Cells7

Disperse1

Acute bacterial1

Colony formation1

Metabolic activity2

Structural2