Discrete enzymes in this pathway include two reductase enzymes: 3-oxoacyl-acyl carrier protein (ACP) reductase (FabG) and enoyl-ACP reductase (FabI). (rcsb.org)
Here, we report gene deletion experiments that support the essentiality of this gene in P. aeruginosa and the identification of a number of small molecule FabG inhibitors with IC50 values in the nanomolar to low micromolar range and good physicochemical properties. (dundee.ac.uk)
There are many possible applications for these genes: you could try to recreate essential gene pathways in-vitro, or purify the proteins for functional studies, or create a synthetic MoClo-compatible Bacillus subtilis genome using these genes and the sgRNAs available from the Bacillus Genetic Stock Center. (freegenes.org)
The codon table used was the FreqB table from " Design Parameters to Control Synthetic Gene Expression in Escherichia coli ", which was used because it was shown to express proteins well and was recommended to us by JCVI. (freegenes.org)
Cpr12, encoded by a gene that was originally proposed to be outside the gene cluster and sharing similarity to proteins annotated as ADC lyase, was revealed to catalyze the elimination of pyruvate to form PABA. (uky.edu)
Biosynthesis4
FabG catalyzes reduction of a 3-oxo-acyl-ACP intermediate during the elongation cycle of fatty acid biosynthesis. (dundee.ac.uk)
Here we present the functional assignment and biochemical characterization of seven proteins, Cpr33-38 and Cpr12, that initiate the biosynthesis of the polyamide. (uky.edu)
Cpr37, which belongs to the adenylation domain protein in the nonribosomal peptide synthase (NRPS), subsequently activates PABA and loads it to holo-Cpr36 Two proteins Cpr34 and Cpr35 work in concert to catalyze decarboxylative condensation between a thioester linked PABA and malonyl- S -acyl carrier protein (ACP) during aromatic polyketide biosynthesis catalyzed by type II polyketide synthases. (uky.edu)
In this scenario, hydride is predicted to be added to the re face to generate the S configuration resulting in the same stereochemical outcome as other 3-oxoacyl-ACP reductase (FabG) from bacterial type II fatty acid synthases.These findings are critical advancement for interrogating the biosynthesis of the unusual chemical components of the family of antibiotics of capuramycin. (uky.edu)
TIGRFam1
Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)
Bacterial2
To date, this is the second bacterial high-molecular weight FabG structurally characterized, following FabG4 from Mycobacterium. (rcsb.org)
3-Oxo-acyl-acyl carrier protein (ACP) reductase (FabG) plays a key role in the bacterial fatty acid synthesis II system in pathogenic microorganisms, which has been recognized as a potential drug target. (dundee.ac.uk)
Pathway1
Here, the structure of the third enzyme in the FAS pathway, 3-ketoacyl-(acyl-carrier-protein) reductase, is reported at a resolution of 2.25 Å. (rcsb.org)
Fatty acid synt1
Inhibitors designed to target FabG proteins and thus prevent fatty acid synthesis may provide a platform for use against multidrug-resistant pathogens including A. baumannii. (rcsb.org)
Catalyzes reduction1
Following condensation, Cpr33 acts as 3-oxoacyl-ACP reductase that catalyzes reduction to the β-hydroxythioester intermediate. (uky.edu)
Coenzyme1
Here, we investigate annotated FabG homologs, finding a low-molecular weight 3-oxoacyl-ACP reductase, as the most likely FASII FabG candidate, and high-molecular weight 3-oxoacyl-ACP reductase (HMwFabG), showing differences in structure and coenzyme preference. (rcsb.org)
Genes1
Owing to its small genome (about 800 protein-coding genes), it relies on the host for many basic biosynthetic processes, hindering the identification of potential antipathogenic drug targets. (rcsb.org)
Characterization1
Structural characterization of 16 FabG-inhibitor complexes by X-ray crystallography revealed that the compounds bind at a novel allosteric site located at the FabG subunit-subunit interface. (dundee.ac.uk)
Genome1
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. (lbl.gov)
Type1
Uncharacterized short-chain type dehydrogenase/reductase y4mP OS=Rhizobium sp. (uu.nl)
Experimental Data1
Hits to curated proteins without experimental data as to their function are never considered high confidence. (lbl.gov)
Function1
Cpr36 is demonstrated to function as a free-standingpeptidyl carrier protein (PCP), which is activated to form holo-protein from the apo-form. (uky.edu)
Find1
GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams ). (lbl.gov)
Short-chain type dehydrogenase1
Y4LA_RHISN (Uncharacterized short-chain type dehydrogenase/reductase y4lA OS=Rhizobium sp. (citrusgenomedb.org)
ENZYME2
GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams ). (lbl.gov)
Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam ). (lbl.gov)
Putative2
10614) putative ABC transport system permease protein BBZA01000001 CDS ARMA_0010 complement(10625. (go.jp)
11353) putative ABC transport system ATP-binding protein BBZA01000001 CDS ARMA_0011 complement(11350. (go.jp)
Ketoacyl1
These results suggest that the synthetic pathway for the fatty acid moiety of rhamnolipids is separate from the general fatty acid synthetic pathway, starting with a specific ketoacyl reduction step catalyzed by the RhlG protein. (nih.gov)
Ribosomal protein L33 [Interproscan]. (ntu.edu.sg)
Hypothetical protein45
4628 hypothetical protein BBZA01000001 CDS ARMA_0005 5077. (go.jp)
5685 hypothetical protein BBZA01000001 CDS ARMA_0006 5873. (go.jp)
7021 hypothetical protein BBZA01000001 CDS ARMA_0007 complement(7097. (go.jp)
7504) hypothetical protein BBZA01000001 CDS ARMA_0008 complement(7590. (go.jp)
11958) hypothetical protein BBZA01000001 CDS ARMA_0012 12170. (go.jp)
578 hypothetical protein BBZA01000002 CDS ARMA_0016 684. (go.jp)
2342 hypothetical protein BBZA01000002 CDS ARMA_0017 2339. (go.jp)
3094 hypothetical protein BBZA01000002 CDS ARMA_0018 3478. (go.jp)
4371 hypothetical protein BBZA01000002 CDS ARMA_0019 complement(4468. (go.jp)
5751) hypothetical protein BBZA01000002 CDS ARMA_0021 complement(5880. (go.jp)
6020) hypothetical protein BBZA01000002 CDS ARMA_0020 6069. (go.jp)
7490 hypothetical protein BBZA01000002 CDS ARMA_0022 complement(7569. (go.jp)
10130) hypothetical protein BBZA01000002 CDS ARMA_0023 10363. (go.jp)
12985 hypothetical protein BBZA01000002 CDS ARMA_0025 13067. (go.jp)
13828 hypothetical protein BBZA01000002 CDS ARMA_0026 13840. (go.jp)
15584 hypothetical protein BBZA01000003 CDS ARMA_0028 complement(2147. (go.jp)
2662) hypothetical protein BBZA01000003 CDS ARMA_0030 complement(2909. (go.jp)
3070) hypothetical protein BBZA01000003 CDS ARMA_0029 3089. (go.jp)
3247 hypothetical protein BBZA01000003 CDS ARMA_0031 3817. (go.jp)
11054 hypothetical protein BBZA01000003 CDS ARMA_0036 11082. (go.jp)
11983 hypothetical protein BBZA01000003 CDS ARMA_0038 complement(11960. (go.jp)
12616) hypothetical protein BBZA01000003 CDS ARMA_0039 12726. (go.jp)
13859 hypothetical protein BBZA01000003 CDS ARMA_0040 13859. (go.jp)
14554 hypothetical protein BBZA01000003 CDS ARMA_0041 14582. (go.jp)
15289 hypothetical protein BBZA01000003 CDS ARMA_0042 15306. (go.jp)
15746 hypothetical protein BBZA01000003 CDS ARMA_0043 complement(15750. (go.jp)
16253) hypothetical protein BBZA01000003 CDS ARMA_0044 complement(16376. (go.jp)
16723) hypothetical protein BBZA01000003 tRNA ARMA_tRNA01 complement(16745. (go.jp)
17762 hypothetical protein BBZA01000003 CDS ARMA_0046 17875. (go.jp)
19373 hypothetical protein BBZA01000003 CDS ARMA_0048 complement(19431. (go.jp)
20651) hypothetical protein BBZA01000003 CDS ARMA_0049 20873. (go.jp)
21793 hypothetical protein BBZA01000003 CDS ARMA_0050 21812. (go.jp)
24549 hypothetical protein BBZA01000003 CDS ARMA_0052 24709. (go.jp)
25812 hypothetical protein BBZA01000003 CDS ARMA_0053 25854. (go.jp)
27638 hypothetical protein BBZA01000003 CDS ARMA_0054 27690. (go.jp)
29561 hypothetical protein BBZA01000003 CDS ARMA_0055 29551. (go.jp)
30963 hypothetical protein BBZA01000003 CDS ARMA_0056 30956. (go.jp)
32158 hypothetical protein BBZA01000003 CDS ARMA_0057 32158. (go.jp)
35130 hypothetical protein BBZA01000003 CDS ARMA_0058 35127. (go.jp)
35501 hypothetical protein BBZA01000003 CDS ARMA_0059 35523. (go.jp)
38051 hypothetical protein BBZA01000003 CDS ARMA_0060 38048. (go.jp)
38443 hypothetical protein BBZA01000003 CDS ARMA_0061 38445. (go.jp)
40466 hypothetical protein BBZA01000003 CDS ARMA_0063 complement(40556. (go.jp)
42784) hypothetical protein BBZA01000004 CDS ARMA_0066 23. (go.jp)
493 hypothetical protein BBZA01000004 CDS ARMA_0067 664. (go.jp)
Synthesis1
In addition, the synthesis of poly-beta-hydroxyalkanoate (PHA) is delayed in this mutant, suggesting that RhlG participates in PHA synthesis, although it is not the only reductase involved in this pathway. (nih.gov)
Pseudomonas1
We found that the soil antifungal bacterium Lysobacter enzymogenes OH11 (OH11) could use T4ASS to deliver the effector protein Le1288 into the cytoplasm of another soil microbiome bacterium Pseudomonas fluorescens 2P24 (2P24). (bvsalud.org)
Bits1
For alignments to characterized proteins (from ublast), scores of 44 bits correspond to an expectation value (E) of about 0.001. (lbl.gov)
Transport1
41314) energy-coupling factor transport system permease protein ecfT BBZA01000003 CDS ARMA_0064 complement(41356. (go.jp)
Functions1
GSU2947 is enriched for 11 functions in 3 categories. (systemsbiology.net)
Domain1
Cache domain, Methyl-accepting chemotaxis protein (MCP) signalling domain [Interproscan]. (ntu.edu.sg)
Search1
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. (lbl.gov)