• METHODS: Hepatocyte nuclear factor 4α expression was tested in posterior capsular opacification rat lens capsules and cell models. (bvsalud.org)
  • CONCLUSIONS: We demonstrated that hepatocyte nuclear factor 4α inhibited epithelial-mesenchymal transition of lens epithelial cells by directly binding to the MMP2 promoter region and inhibiting the expression of MMP2, thus leading to retardation of posterior capsular opacification formation and development, suggesting that hepatocyte nuclear factor 4α is a potential therapeutic target for posterior capsular opacification. (bvsalud.org)
  • Musil's team found that adding active TGF-β to their culture system induced the lens epithelial cells to differentiate into either myofibroblasts or lens fiber cells, while adding a TGF-β inhibitor reduced differentiation. (nih.gov)
  • 3. The role of nuclear factor kappa B in lens epithelial cell proliferation using a capsular bag model. (nih.gov)
  • 11. Lens epithelial cell proliferation in human posterior capsule opacification specimens. (nih.gov)
  • 15. SiRNA Targeting mTOR Effectively Prevents the Proliferation and Migration of Human Lens Epithelial Cells. (nih.gov)
  • Launch Anterior subcapsular cataract (ASC) and capsule opacification are both due to extreme proliferation and differentiation of zoom lens epithelial cells (LECs)[1C4]. (conferencedequebec.org)
  • Posterior capsule opacification (PCO), also called secondary cataract, outcomes from proliferation and migration of residual zoom lens epithelial cells over the posterior capsule. (conferencedequebec.org)
  • Proliferation and epithelial-mesenchymal changeover (EMT) of LECs play important functions in the pathogenesis of ASC and capsule opacification[4, 11, 12]. (conferencedequebec.org)
  • Conditional knockout of Spry2 in mouse zoom lens enhances TGF-induced EMT, while Spry2 overexpression inhibits LEC proliferation and differentiation[34C36]. (conferencedequebec.org)
  • It inhibits proliferation and invasion of bladder cancer cells by downregulating E2F3. (bvsalud.org)
  • In addition, the presence of TGF-β was associated with the migration of lens cells, a requirement for accumulation at the back of the lens capsule and secondary cataract. (nih.gov)
  • Using their lens culture system, they found that blocking the downstream p38 kinase pathway with an inhibitor, prevented myofibroblast formation. (nih.gov)
  • Blocking the p38 kinase pathway, either with direct inhibitors of the p38 MAP kinase or a small-molecule therapeutic that also inhibits the activation of p38, prevented TGFβ from inducing epithelial-myofibroblast transition and cell migration but did not prevent fiber cell differentiation. (nih.gov)
  • The FERM protein Expanded is one of the main upstream Hippo signalling regulators in Drosophila as it promotes Hippo kinase signalling and directly inhibits Yorkie. (sdbonline.org)
  • Four to 12 percent of people undergoing cataract surgery to replace a cloudy lens with a clear artificial one develop posterior capsule opacification (PCO). (nih.gov)
  • Preventing posterior capsule opacification would spare thousands of people from needing laser capsulotomy, a procedure with potential risks that costs the U.S. more than $150 million annually," said Houmam Araj, Ph.D., lens and cataract program director at NEI. (nih.gov)
  • During cataract surgery, a hole is made in the front of the lens capsule, the outer layer that surrounds the lens. (nih.gov)
  • Transforming growth issue (TGF)-induced epithelial-mesenchymal move (EMT) of lens epithelial cells (LECs) performs an integral role in the pathogenesis of anterior subcapsular cataract (ASC) and capsule opacification. (conferencedequebec.org)
  • Cataract, an eye disease that threatens the health of millions of people, brings about severe economic burden for patients and society. (bvsalud.org)
  • Similarly, inhibiting the MTOR pathway prevented the formation of lens fiber cells. (nih.gov)
  • Thus, phospho-dependent Expanded turnover ensures a tight coupling of Hippo pathway activity to epithelial architecture. (sdbonline.org)
  • 13. MicroRNA-204-5p regulates epithelial-to-mesenchymal transition during human posterior capsule opacification by targeting SMAD4. (nih.gov)
  • Specifically, it regulates the organization of apical plasma membrane in epithelial cells, disk morphogenesis during early retinal development, MAPK and Akt signaling pathways, and in cholesterol metabolism. (nih.gov)
  • Our results in human zoom lens tissues and epithelial cells claim that Spry2 could become a book therapeutic focus on for the avoidance and treatment of ASC and capsule opacification. (conferencedequebec.org)
  • Methods: A total of 36 cases of bladder cancer tissue specimens and 10 cases of normal bladder epithelial tissue specimens were collected from Tangdu Hospital of Air Force Medical University during February 2018 and December 2018. (bvsalud.org)
  • 7. Selenium functionalized intraocular lenses inhibit posterior capsule opacification in an ex vivo canine lens capsular bag assay. (nih.gov)
  • 9. Prevention of posterior capsule opacification by the induction of therapeutic apoptosis of residual lens cells. (nih.gov)
  • 10. [Lens fiber differentiation in rats posterior capsule opacification]. (nih.gov)
  • 12. Effect of bag-in-the-lens implantation on posterior capsule opacification in human donor eyes and rabbit eyes. (nih.gov)
  • 17. Lens cell targetting for gene therapy of prevention of posterior capsule opacification. (nih.gov)
  • 19. An experimental study on the effects of curcumin on posterior capsule opacification in young rabbit eyes. (nih.gov)
  • Nevertheless, the function of Spry2 in individual ASC and capsule opacification development is not studied, as well as the molecular system of Spry2-governed TGF signaling in individual lens continues to be largely unknown. (conferencedequebec.org)
  • BACKGROUND/AIMS Autologous serum drops have been reported to be beneficial in keratoconjunctivitis sicca (KCS) and persistent epithelial defects (PED). (bmj.com)
  • In ophthalmology, there have been reports of the use of autologous serum for diseases of the external eye particularly keratoconjunctivitis sicca (KCS) and persistent epithelial defects (PED). (bmj.com)
  • 2. An in vitro model of posterior capsular opacity: SPARC and TGF-beta2 minimize epithelial-to-mesenchymal transition in lens epithelium. (nih.gov)
  • 20. Bit1-a potential positive regulator of epithelial-mesenchymal transition in lens epithelial cells. (nih.gov)
  • The liver additionally produces small quantities of different plasma lipoproteins, similar to low-density Vitamin A is the precursor of retinal, which is required for the synthesis of rhodopsin in the eye. (gov.ph)
  • This happens when residual lens epithelial cells left behind from the old, emulsified lens accumulate at the back of the lens capsule and transform into one of two types of cells that cause problems: lens fiber cells and myofibroblasts. (nih.gov)
  • Myofibroblasts generate large amounts of molecules outside the cell, creating a matrix that causes the lens capsule to wrinkle, which obstructs vision. (nih.gov)
  • Previous studies have shown that when active TGF-β is overexpressed in the intact lens, myofibroblasts form. (nih.gov)
  • Looking at the distribution of cell types within the culture, lens fiber cells tend to congregate in densely packed multilayered islands while myofibroblasts form a single layer fringe surrounding those islands. (nih.gov)
  • PCO is caused by residual lens cells undergoing one of two very different cell fates: either transdifferentiating into myofibroblasts or maturing into lens fiber cells. (nih.gov)
  • We show here for the first time that TGFβ can induce purified primary lens epithelial cells within the same culture to undergo differentiation into either lens fiber cells or myofibroblasts. (nih.gov)
  • Laser in situ keratomileusis (LASIK) and its variant, femtosecond LASIK (FS-LASIK), despite their success in providing independence from corrective eyeglasses (spectacles) and contact lenses, has continued to be associated with rare complications, including flap-related complications, postoperative dry eye, denervation of corneal nerves, and biomechanical instability leading to corneal ectasia. (medscape.com)
  • Fewer corneal nerves are severed during creation of the pocket and removal of the lenticule, so dry-eye symptoms and loss of corneal sensitivity may occur less frequently after SMILE. (medscape.com)
  • Nonetheless, the creation of an interface between the flap and the underlying corneal stroma can lead to flap-related and flap-stromal interface complications such as flap dislocation, infectious keratitis, diffuse lamellar keratitis and epithelial ingrowth (EI). (bmj.com)
  • In addition to shedding light on how secondary cataracts form, these findings present some exciting insights into the growth factor TGF-beta that have implications far beyond the eye," he said. (nih.gov)
  • However, due to the creation of a potential interface between the flap and the underlying stroma, interface complications such as infectious keratitis, diffuse lamellar keratitis and epithelial ingrowth may occur. (bmj.com)
  • On microscopy, staining for proteins exclusive to crystallin shows that adding TGF-β induced lens fiber cell (green). (nih.gov)
  • The study appears in Molecular Biology of the Cell and was funded by the National Eye Institute, part of the National Institutes of Health. (nih.gov)
  • Next, they assessed whether TGF-β had a direct effect on lens epithelial cell differentiation, or if it was indirectly enabled by molecular signaling downstream of TGF-β activation. (nih.gov)
  • 5. Prognostic significance of nuclear factor-kappaB p105/p50 in human melanoma and its role in cell migration. (nih.gov)
  • Although TGFβ has been strongly implicated in lens cell fibrosis, the factors responsible for the latter process have not been identified. (nih.gov)
  • Hippo signalling integrates diverse stimuli related to epithelial architecture to regulate tissue growth and cell fate decisions. (sdbonline.org)
  • An inflamed cell inhibits nutrients getting into the cell, toxins being removed from the cell and energy being produced. (nutrishield.com)
  • Free radicals also damage cell membranes in the brain, the lining of arteries leading to heart disease, the lens of the eye leading to blindness, and proteins in the body. (nutrishield.com)
  • GA-D significantly inhibited β -galactosidase (a senescence-associated marker) formation, in a dose-dependent manner, with doses ranging from 0.1 μ M to 10 μ M, without inducing cytotoxic side-effects. (hindawi.com)
  • SRPIN340 inhibited CNV formation in a dose-dependent manner. (molvis.org)
  • Compared with the vehicle, SRPIN340 significantly decreased the protein levels of VEGF, MCP-1, ICAM-1, and consequently inhibited macrophage infiltration. (molvis.org)
  • RESULTS Autologous serum was used in 15 eyes of 13 patients with PED and 11 eyes of nine patients with KCS. (bmj.com)
  • They provide antibacterial factors, prevent desiccation of the cornea, and contain substances like epidermal growth factor (EGF), fibronectin, basic fibroblast growth factor (bFGF), and factors that are thought to maintain epithelial health. (bmj.com)
  • During zoom lens development, Spry2 adversely modulates ERKs to permit zoom lens vesicle separation[33]. (conferencedequebec.org)