Extraction dna bacterial. Medical search. Frequent questions

Here, we report, for the first time, the cross-generational transmission of specific bacterial strains from an FMT donor to a pregnant patient with CDI and further to her child, born at term, 26 weeks after the FMT treatment. (biomedcentral.com)
The evidence for cross-generational strain transfer following FMT provides novel insights into the dynamics and engraftment of bacterial strains from healthy donors. (biomedcentral.com)
The data suggests FMT treatment of pregnant women as a potential strategy to introduce beneficial strains or even bacterial consortia to infants, i.e., neonatal seeding. (biomedcentral.com)
In total, 145 endophytic bacterial strains were isolated and further grouped using either ERIC-PCR or BOX-PCR, resulting in 76 groups composed of different genera predominantly belonging to the Gammaproteobacteria. (biomedcentral.com)
ceptible to urinary tract infections with The second phase of our study included multiresistant bacterial strains [ 7-9 ]. (who.int)
The emergence of certain bacterial strains resistant to antibiotics has become a major public health problem, hence the need to develop new antibiotic molecules. (bvsalud.org)
There is no cross-reactivity with non-tuberculous mycobacteria, and TB and rifampicin resistance were correctly detected in the presence of non-tuberculous DNA or mixed susceptible and resistant strains. (bvsalud.org)
Results obtained from Restriction fragment length polymorphism typing show that the mahority of circulating Mycobacterium tuberculosis strains in Sri Lanka belong to a limited number of families, but the degree of IS6160 DNA polymorphism among strains were high. (who.int)
In bacterial isolates of prisoners and ex-prisoners from the general population, there were 2 strains, which had identical banding patterns, while there were clear similarities between several isolates. (who.int)

As central microbiota functions are determined by bacterial community networks, it is important to gain insight into the principles that govern bacteria-bacteria interactions. (nature.com)
DNA extraction is frequently a preliminary step in many diagnostic procedures used to identify environmental viruses and bacteria and diagnose illnesses and hereditary diseases. (wikipedia.org)
However, much is unknown about the effects of indigenous bacteria on microalgal growth and the characteristics of bacterial communities associated with microalgae in microalgae-effluent culture. (biomedcentral.com)
To enhance their own growth, microalgae may be able to selectively stimulate specific bacterial groups from among the in situ indigenous bacterial community found in wastewater effluent (i.e., microalgae growth-promoting bacteria: MGPB). (biomedcentral.com)
2013. Identification of active oxalotrophic bacteria by bromodeoxyuridine DNA-labeling in a microcosm soil experiment. (unine.ch)
To confirm which media and which bacterial colony densities can enrich certain kinds of bacteria, we selected eight common media and used them to enrich the gut microorganisms on agar plates. (springeropen.com)
We investigated how the nutritional components and densities influence the composition and diversity of the bacterial community, which bacteria could be enriched and the conditions of nutritional composition or density that allowed enrichment, and what the interaction was among bacteria at various densities. (springeropen.com)
and to extract DNA from any bacteria found to develop a metabolic model for the system. (sciencedaily.com)
The team also collected samples of bacteria and tested a new technique for DNA extraction. (sciencedaily.com)
Centrifuge the bacterial cultures for 10 min at 1000 x g to pellet the bacteria. (integra-biosciences.com)
If shown to be effective, the widespread use of mouthwash will reduce the prevalence of oropharyngeal gonorrhoea, which plays key role in driving the emergence of gonococcal antimicrobial resistance through DNA exchange with oral commensal bacteria. (biomedcentral.com)
Using DNA extracted from bacteria in the dental plaque of mother/child pairs, a sequence of the S. mutans spaP gene was amplified using PCR. (bvsalud.org)
Spore extraction and bacteria with infected animals or their contaminated identification products [ 4 ]. (who.int)
This study determined the prevalence of bacterial contamination of mobile phones of health workers and the predisposing factors, in order to ascertain the risk of transmission of pathogenic bacteria through mobile phones. (bvsalud.org)
Bacterial DNA gyrase, a type II DNA topoisomerase found in all bacteria is a proven target for antibacterial chemotherapy. (bvsalud.org)

These kits contain reagents & consumables required for extraction, including pre-filled reagent cartridges and all disposable plastics which are essential to use with the AnaPrep system. (biochain.com)
The Bacterial Genomic Dna Extraction Protocol reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (abeurope.info)
TRIzol reagent, LA Taq DNA polymerase and lipid Lipofectamine 2000 (Invitrogen Life Technologies, Carlsbad, CA, USA) were used. (spandidos-publications.com)

The specific method used to extract the DNA, such as phenol-chloroform extraction, alcohol precipitation, or silica-based purification. (wikipedia.org)
Purification: After the DNA is precipitated, it is usually further purified by using column-based methods. (wikipedia.org)
DNA purification from detergents, proteins, salts, and reagents is used during the cell lysis step. (wikipedia.org)
Microbial cells are separated from the mat matrix prior to DNA extraction and purification. (jove.com)
DNA purification procedures that use spin columns can result in high salt levels, which can inhibit enzyme activity. (neb.com)
If your digestion results in several DNA fragments, and you are only interested in using one of them in your cloning workflow, then the most common way to isolate the fragment of interest is by running the digestion in a gel, excising the band from the gel, and isolating the DNA by column purification (e.g. (neb.com)
The use of silica membrane-based DNA purification kits is a convenient way to prepare plasmid DNA samples for cloning or subsequent analysis, e.g. sequencing or restriction analysis. (integra-biosciences.com)
MACHEREY-NAGEL's NucleoSpin 96 Plasmid kit is designed for high throughput purification of high-copy plasmid DNA from E. coli in a 96 well plate format. (integra-biosciences.com)
MACHEREY-NAGEL's NucleoSpin 96 Plasmid kit and NucleoVac 96 Vacuum Manifold are a proven method for high throughput plasmid DNA purification. (integra-biosciences.com)
The ASSIST PLUS pipetting robot is used to automate the pipetting steps of the MACHEREY-NAGEL NucleoSpin 96 Plasmid kit protocol for plasmid DNA purification. (integra-biosciences.com)
In this study, with different bacterial cocktail mixes, analyzed as pure or spiked in a synthetic fecal matrix, we evaluated the performances of 6 DNA extraction methods using various cells lysis and purification techniques, from quick and easy, to more time-consuming and gentle protocols, including a portable method for on-site application. (altmetric.com)
88 full texts were evaluated before final critique based on study power, sample contamination avoidance, storage, purification process, DNA extraction/analysis, and consideration of maternal health and other potential confounders. (bvsalud.org)
For the DNA extraction and purification from the clinical samples two methods, the standard phenol extraction procedure desctibed by Sambrook etal (1982) and the guanidinium thiocyanate method described by Boom et al(1990) were used. (who.int)

Our microbial DNA extraction kits provide you with high yields of high-quality DNA and enable efficient lysis, thereby preventing bias towards abundant species. (qiagen.com)
Analysis showed that the gut bacterial composition of samples from HwA was less diverse than that of samples from HwoA and had a lower abundance of Roseburia , Barnesiella , Butyricicoccus , Odoribacter , and Butyricimonas species, most of which were short-chain fatty acids-producing microbes. (frontiersin.org)
The Species identifications were identical in only 61.5%, when absence of positive culture is most frequently due to previ- conventional techniques and DNA sequencing were used. (cdc.gov)
The enriched core bacterial families and genera were functions of the microalgal species and effluents. (biomedcentral.com)
These results suggest that certain members of the bacterial community promote the growth of their "host" microalgal species. (biomedcentral.com)
Another study, by Matthew Meyerson of Harvard Medical School and colleagues, identified a novel bacterial species , Bradyrhizobium enterica, in patients with a colitis syndrome. (the-scientist.com)
The microbiota associated with many species of insects offer an extraction challenge as they are frequently surrounded by an armored exoskeleton, inhibiting disruption of the tissues within. (harvard.edu)
In recent years, microbial culturomics has been a culturing approach that uses multiple techniques, such as MALDi-TOF mass spectrometry and 16S rRNA sequencing, for the identification of bacterial species. (springeropen.com)
Furthermore, this kit allowed an accurate detection, by Nanopore sequencing, of almost all the bacterial species present in a complex mock community. (altmetric.com)
The laboratory (18.8%, 21/112) and medical wards (16.1%, 18/112) had the highest bacterial contamination of mobile phones (p=0.041), and more bacterial species were isolated from smartphones (68.8%, n=77/112) than keypad phones (31.2%, n=35/112) (p=0.032). (bvsalud.org)
DNA probes are available for several species. (medscape.com)

There are many different methods for extracting DNA, but some common steps include: Lysis: This step involves breaking open the cells to release the DNA. (wikipedia.org)
Breaking the cell membranes open exposes the DNA along with the cytoplasm within (cell lysis). (wikipedia.org)
The alkaline lysis-based miniprep protocol typically yields 5-15 μg of plasmid DNA from 1.5ml overnight cultures. (integra-biosciences.com)
The sensitivity of the PCR method is largely dependent on the efficiency of the DNA extraction procedure which, could probably be improved by modifications of the lysis technique. (who.int)

Phenol-chloroform extraction in which phenol denatures proteins in the sample. (wikipedia.org)
The Boom's method was preferred to phenol/chloroform extraction method as most or all of the inhibitory substances in clinical samples were effectively removed by this method. (who.int)

Rhizoplane bacterial composition was characterized with 16S rRNA gene sequencing, while bacterial abundance was determined on a subset of trees through flow cytometry. (nature.com)
Combining pangenomic and both metagenomic and metatranscriptomic profiling revealed a striking divergence in the vertical distribution, genomic composition, metabolic potential, and predicted lifestyle strategies of geographically co-located members of the SAR324 bacterial clade. (biomedcentral.com)
The induced dysbiosis was characterized by alterations in bacterial abundance, composition, and diversity in our animal models. (aacrjournals.org)
While bacterial community composition recovered using Illumina 16S rRNA amplicon sequencing was not detectably biased by any method, the quantity of bacterial DNA varied drastically, reducing the number of samples that could be amplified and sequenced. (harvard.edu)
Using the Illumina HiSeq platform, we analyzed the composition and diversity of the culture-enriched gut bacterial community. (springeropen.com)
DNA extraction method plays a significant role when defining bacterial community composition in the marine invertebrate Echinometra mathaei. (nyu.edu)

Our results clearly demonstrate that direct DNA extraction methods can significantly influence the DNA quantity, purity, and observed community patterns of microbiota in activated sludge. (knaw.nl)
Here, we focused on the growth and metabolic interactions of the Oligo-Mouse-Microbiota (OMM 12 ) synthetic bacterial community, which is increasingly used as a model system in gut microbiome research. (nature.com)
BALF from paediatric and adult CF patients and paediatric disease controls undergoing clinically indicated bronchoscopy was analysed for total bacterial load and for microbiota by 16S rDNA sequencing. (ersjournals.com)

When the sample of S. bongori was 10,000 cells or fewer, the abundance of DNA from other microbial taxa exceeded 50 percent of the sequences. (the-scientist.com)

These results suggest that the three microalgae produced and supplied organic carbon that supported bacterial growth in the effluent. (biomedcentral.com)

Dna Extraction Laboratories manufactures the bacterial genomic dna extraction protocol reagents distributed by Genprice. (abeurope.info)

The HwA subgroup analysis indicated a trend toward restoration of the HwoA bacterial microbiome over time after appendectomy. (frontiersin.org)
It's really important to sequence a negative extraction control," said Patrick Schloss , a microbiome researcher at the University of Michigan who did not participate in this study. (the-scientist.com)

FLICKR, CIMMYT Researchers studying microbiomes can do their best to prevent contamination, but a new study reveals widespread, low-level contamination in DNA extraction kits. (the-scientist.com)
Studies have even found bacterial DNA in ultrapure water, and just a few weeks ago researchers pointed out ubiquitous contamination in next generation sequencing runs. (the-scientist.com)
Although Methylobacteriaceae and Shingomonadaceae popped up in some of the DNA extraction kits Walker's group tested, Lee said that she and her team are very aware of contamination concerns-especially given that her work involves low biomass samples-and that they performed the right controls. (the-scientist.com)
The estimated residual risk of contamination of blood products with bacterial agents is 1 in 5,000 for platelets and 1 in 30,000 for red blood cells. (medscape.com)
Also important is the duration of storage, which has a direct correlation with the likelihood of bacterial contamination. (medscape.com)
A meta-analysis of 22 studies of bacterial contamination rate estimates for apheresis (AP), platelet-rich plasma (PRP), and buffy coat (BC) collection methods found an overall mean contamination rate of 0.51 per 1000 components (95% confidence index [CI], 0.38-0.67). (medscape.com)
Probable contamination - The blood culture from the recipient is negative or could not be done, but there is definite bacterial growth in the donor blood product. (medscape.com)

To overcome these problems, molecular techniques strate a clear benefit of molecular identification, particularly using broad-range DNA primers for amplification of bac- in cases of blood culture-negative endocarditis and of pos- sible endocarditis, to confirm or invalidate the diagnosis. (cdc.gov)
Respiratory samples from 367 patients suspected of bacterial pneumonia were analysed by PCR amplification of Pneumocystis jiroveci . (biomedcentral.com)
PCR interference may be due to issues with DNA extraction and/or amplification, and results in severe underestimation of bacterial concentrations. (hawaii.edu)
No cross-amplification was observed when the analytical specificity was tested with human, amebal ( Acanthamoeba castellanii ATCC 30010), and bacterial DNA ( Technical Appendix ). (cdc.gov)
The objective of the study was to develop a rapid method to detect Mycobacterium tuberculosis from clinical samples and the applicability of DNA amplification techniques to a developing country like Sri Lanka. (who.int)
In conclution, DNA amplification is a rapid, reliable and accurate method with a high degree of sensitivity and specificity for the detection of Mycobacterium tuberculosis DNA sequences and it can replace the conventional culture method in the diagnosis of extra pulmonary tuberculosis and tuberculosis meningitis except in the situation when antibiotic sensitivity results are required. (who.int)

Furthermore, the total numbers of bacterial 16S rRNA genes in the 7-day microalgae-effluent cultures were 109‒793 times the initial numbers. (biomedcentral.com)
Antimicrobial resistance occurs through different mechanisms, which include spontaneous (natural) genetic mutations and horizontal transfer of resistant genes through deoxyribonucleic acid (DNA). (who.int)

No matter the sample origin, you need high yields of microbial DNA and unbiased results for analysis of microbial communities. (qiagen.com)
Bacterial communities in aquatic environments may either stimulate or inhibit microalgal growth. (biomedcentral.com)
The in situ indigenous bacterial communities in the effluents therefore promoted the growth of the three microalgae during 7-day cultures. (biomedcentral.com)
An increased understanding of the spatial and temporal variation of bacterial communities is critical when assessing delivery of ecosystem services and their role in soil biogeochemical processes. (frontiersin.org)
CF sputum samples are now known to contain complex bacterial communities, including facultative and obligate anaerobic organisms [ 10 - 12 ]. (ersjournals.com)
Although laboratory and analysis techniques should be standardized across diverse sample types as much as possible, minimal modifications such as these will increase the number of environments in which bacterial communities can be successfully studied. (harvard.edu)
In this incident density sampled case control study of 189 children followed from 2-months to 5-years, we use the salivary bacteriome to 1) prospectively test the ecological hypothesis of ECC in salivary bacteriome communities and 2) identify co-occurring salivary bacterial communities predicting future ECC. (researchsquare.com)
Bacterial communities which assemble before 12-months of age can promote or inhibit an ecological succession to S. mutans dominance and cariogenesis. (researchsquare.com)

Then, we calculated the numbers of bacterial colonies and collected the bacterial culture mixtures from each kind of medium. (springeropen.com)

Compared with those in samples from HwoA, the abundance correlation networks in samples from HwA displayed more complex fungal-fungal and fungal-bacterial community interactions. (frontiersin.org)
The aim of the current study was to evaluate the clinical significance and prevalence of a PCR positive signal in a broad spectrum of clinical samples from HIV-negative patients with suspected bacterial pneumonia by means of an age and sex matched nested case-control study. (biomedcentral.com)
The remaining 106 respiratory samples represented all specimens submitted for investigation of bacterial pneumonia during a one-week period in August 1999, at the Department of Clinical Microbiology, Herlev University Hospital. (biomedcentral.com)
Taking into account our results, we recommend Fast as the most suitable DNA extraction method for activated sludge samples used for bacterial community studies. (knaw.nl)
Yet, variation in the physical properties of environmental samples demands that optimal DNA extraction techniques be explored for each new environment. (harvard.edu)
This study evaluated the use of a commercial stool storage kit for bacterial preservation in cow faecal samples compared to unpreserved stools placed into refrigeration at different time-points. (imperial.ac.uk)
After 2 weeks, DNA was extracted from the samples and analysed using endpoint PCR, revealing a sub-clinical infection with Salmonella spp. (imperial.ac.uk)
The collection device contains a stabilizing solution which maintains the DNA levels in human saliva samples by protecting DNA from degradation and inhibiting bacterial growth over storage time. (qiagen.com)
Samples collected with the PAXgene Saliva Collector have stable DNA levels for up to 24 months at temperatures up to 25°C. This longevity is possible as DNA is preserved from chemical and enzymatic degradation and bacterial growth is inhibited. (qiagen.com)
To take the best advantage of long-read sequencing, DNA extraction methods must be able to isolate pure high molecular weight (HMW) DNA from complex metagenomics samples, without introducing any bias. (altmetric.com)
Analyses of coastal water samples has demonstrated that the PCR interference is associated with the DNA extraction step. (hawaii.edu)
Experiments with seeded enterococci and control DNA demonstrated that the DNA was lost during the DNA extraction step in 40% of coastal samples. (hawaii.edu)
Acidification of samples to pH 3.5 was able to alleviate the issue, suggesting that the loss of DNA is due to precipitation with suspended coralline sand particles during the rapid DNA extraction protocol. (hawaii.edu)
Currently we are testing samples seeded with different concentrations of coralline sand (Hawaii) and silica sand (North Carolina) to determine whether the DNA loss is only associated with coralline sand. (hawaii.edu)
Several studies stored samples under conditions that may selectively impact bacterial DNA preservation, others used preculture reducing reliability. (bvsalud.org)
Objective: To detect the presence and transmission of S. mutans carrier of the spaP gene in samples of bacterial plaque in mother/child pairs from municipal child education centers, and the possible association with dental caries. (bvsalud.org)
In Fiji, when stool samples are collected, most pathology laboratories routinely screen for parasites, viruses and bacterial pathogens such as Salmonella and Shigella but not for Campylobacter . (who.int)

For the current protocol, the bacterial cultures are grown in tubes and transferred into a Culture Plate. (integra-biosciences.com)
We also provide training for students, researchers and collaborators in the techniques of medium (agar/broth) preparation for bacterial cultures, antibiotic susceptibility testing (minimum inhibitory concentration determination), molecular cloning and population analysis for hetero-VISA detection. (ukm.my)

The basic idea is to use a nucleic acid probe to hybridize nuclear DNA from either interphase cells or metaphase chromosomes attached to a microscopic slide. (wikipedia.org)
The device is part of a comprehensive pre-analytical workflow through nucleic acid extraction and analyses, contributing to reliability and reproducibility of your test results. (qiagen.com)
The PAXgene Saliva Collector is part of a comprehensive pre-analytical workflow for human saliva collection, stabilization, transport and storage through nucleic acid extraction and analyses. (qiagen.com)

We assessed the alterations in gut bacterial and fungal populations associated with a history of appendectomy. (frontiersin.org)

The pure culture of the bacterial strain was grown under aerobic conditions on tryptic soy broth agar plates. (asm.org)

The bacterium was best preserved when the stool was stored in the commercial kit at ambient temperature and re-homogenised immediately prior to DNA extraction. (imperial.ac.uk)

The effectiveness of three commercially available direct DNA isolation kits (Mobio, Fast, Qiagen) and one published direct DNA extraction protocol (Bead) for extracting bacterial DNA from different types of activated sludge was investigated and mutually compared. (knaw.nl)
It's worth noting that some variations on these steps may be used depending on the specific DNA extraction protocol. (wikipedia.org)
We provide an improved protocol for extracting high molecular weight DNA from hypersaline microbial mats. (jove.com)
Automation of the protocol on an ASSIST PLUS pipetting robot with a VIAFLO 12 channel 1250 μl electronic pipette offers an easy and efficient way to extract and purify plasmid DNA with minimal hands-on time, guaranteeing perfect and reproducible liquid handling while protecting the user from repetitive strain injuries. (integra-biosciences.com)
The intent of this project was to investigate a standardized sampling protocol for use with bTEFAP that would ultimately provide occupational scientists a novel and effective tool in the quest to characterize bioaerosol exposure and its subsequent relationship to worker health Methods: Four filter types (Millipore Durapore Membrane Filter, SKC water-soluble gelatin filter, SKC PTFE, SKC PVC) were prescreened for low-background DNA content using Pyrosequencing. (cdc.gov)

The presence of microbial DNA in laboratory reagents is nothing new. (the-scientist.com)
Martin Laurence , the founder of Montreal-based ShipShaw Labs, which develops bioinformatics tools, said that sequencing studies would greatly benefit from kits that are free of any microbial DNA. (the-scientist.com)
Exoskeletal pulverization and tissue digestion increased the reliability of extractions, suggesting that these steps should be included in any study of insect-associated microorganisms that relies on obtaining microbial DNA from intact body segments. (harvard.edu)

The commensal bacterial community residing in the human intestinal tract has attracted much attention in recent years and may represent a novel therapeutic target in various disease states [ 1 ]. (biomedcentral.com)

Furthermore, inhibitors and background DNA and RNA can lead to inaccurate quantification of nucleic acids and inhibit your downstream applications. (qiagen.com)
However, the main limitations of such a tools are that they extract low levels of DNA and they do not remove the inhibitors of polymerase chain reaction (PCR). (mdpi.com)
The recommended ten-fold dilution of the DNA extracts to remove PCR inhibitors did not alleviate the problem. (hawaii.edu)
Our objective is designing novel DNA Gyrase inhibitors using Quantitative StructureActivity Relationships and Structure-Based Drug Design Approaches. (bvsalud.org)

Fast and Mobio generated high amounts of good quality DNA compared to Bead and Qiagen. (knaw.nl)
The PAXgene Saliva Collector is compatible with existing QIAGEN automated and manual extraction kits. (qiagen.com)
It is compatible with existing QIAGEN automated extraction kits, such as the QIAsymphony DNA Midi Kit on the QIAsymphony SP Instrument and the QIAamp DNA Mini Kit on the QIAcube (classic and Connect), as well as for manual extraction with the QIAamp DNA Mini and Puregene Cell Kits. (qiagen.com)
The miRNeasy Mini kit serum total RNA extraction kit was from QIAGEN Inc. (Hilden, Germany). (spandidos-publications.com)

If an enzyme is sensitive to bacterial methylation you may need to grow the substrate DNA in a methylation-deficient bacterial strain. (neb.com)

Evaluation of bacterial sampling methods for use with the bacterial tag-encoded flexible (FLX) amplicon pyrosequencing (bTEFAP) technique. (cdc.gov)
Tag-encoded FLX amplicon pyrosequencing analyses utilized Roche 454 FLX instrument with DNA extraction, massively parallel bTEFAP and bacterial identification data analysis was performed at the Research and Testing Laboratory (Lubbock, TX). (cdc.gov)

Inhibition, negative PCR mixture, and extraction controls were systematically tested. (cdc.gov)

Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)
Amongst the 6 tested methods, the Quick-DNA HMW MagBead Kit (Zymo Research) was considered as the most suitable for Nanopore sequencing and would be recommended for bacterial metagenomics studies using this technology. (altmetric.com)
The methods are relatively simple, and involve: 1) sample filtration, 2) rapid DNA extraction by bead beating, and 3) analyses of crude DNA extracts by qPCR for concentrations of enterococci. (hawaii.edu)

Sequencing of 16S rDNA, and of sodA int and rpoB int in matically, and organisms frequently missed with the use of some cases, was applied to DNA from heart valves of 46 earlier blood culture techniques are now recognized. (cdc.gov)
A merase (12) sequencing when 16S rDNA sequences were ccording to the earliest published report on the sub- not sufficiently discriminating, was used here 1) to evalu- ject, the prevalence of blood culture-negative endo- ate the bacterial content of 46 resected heart valves from carditis once ranged from 2.5% to 31% (1). (cdc.gov)
The DNA quantity and purity were determined using real-time PCR targeting the bacterial 16S rDNA gene. (knaw.nl)

However, there is still limited research into spatial and temporal variation patterns of bacterial community assemblages in mangrove soils. (frontiersin.org)

In a study on cystic fibrosis, his team found Pseudomonas DNA present in a commercial kit. (the-scientist.com)

The purified DNA can then be used for downstream applications such as PCR, sequencing, or cloning. (wikipedia.org)

In this study, we examine the efficacy of several commonly used protocols for extracting bacterial DNA from ants. (harvard.edu)

Although the use of polycation to complex and purify DNA has been described in the literature, elution often requires a high ionic strength or pH levels not compatible with molecular analyses. (mdpi.com)
Alternatively, a centrifugation step can be used to purify the DNA by spinning it down to the bottom of a tube. (wikipedia.org)

If you do not have Bicoid DNA, it is acceptable to substitute another non-human non-fungal control DNA in this assay along with the appropriate primers and probes. (cdc.gov)
2013. Gains of bacterial flagellar motility in a fungal world. (unine.ch)

Description: The Cell Biolabs Bacterial Protein Extraction Reagents contain a gentle, nonionic detergent formulation which quickly extracts functional, recombinant protein from E. coli without mechanical disruption. (abeurope.info)

In addition to the comparison of the quality, quantity and purity of the extracted DNA, the performance obtained when doing Nanopore sequencing on a MinION flow cell was also tested. (altmetric.com)

Detection of Pneumocystis-DNA was associated with a worse prognosis: seven (44%) of patients with positive PCR died within one month compared to nine (14%) of the controls (p = 0.01). (biomedcentral.com)
In order to increase sensitivity in non-invasive specimens, several experimental studies have evaluated PCR detection of P. jiroveci DNA as a potential diagnostic test. (biomedcentral.com)
dc.title: PCR based detection techniques and DNA finger printing by restriction fragment analysis of mycobacterium tuberculosis dc.contributor.author: MAGANAARACHCHI, DN dc.description.abstract: In Sri Lanka definitive diagnosis of tuberculosis depends on the culture of mycobacteria, but the slow growth of the organism delays the diagnosis. (who.int)

Early-life bacterial interactions predisposed children to ECC, supporting a time-dependent interpretation of the ecological hypothesis. (researchsquare.com)

Metabolic network reconstruction in combination with metabolomics analysis of bacterial culture supernatants provided insights into the metabolic potential and activity of the individual community members. (nature.com)

The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. (wikipedia.org)

In addition to DNA stabilization, a research study could show that SARS-CoV-2 (severe acute respiratory syndrome coronavirus type 2)-derived RNA copy numbers are preserved when saliva is collected in the PAXgene Saliva Collector. (qiagen.com)

So far, colistin has been best characterized with respect to its structure and biosynthesis, antibacterial mechanism and bacterial resistance, and toxicity and derivatives. (hindawi.com)

For the chemical method, many different kits are used for extraction, and selecting the correct one will save time on kit optimization and extraction procedures. (wikipedia.org)
It is a molecular method used, among other things, to recognize and count particular bacterial groupings. (wikipedia.org)
To this day, the most effective method dracunculiasis treatment involves extraction by wrapping the worm around a stick, as is seen in the image below. (medscape.com)

For example, in the case of bacterial cells, a solution of detergent and salt (such as SDS) can be used to disrupt the cell membrane and release the DNA. (wikipedia.org)

It has been determined that colistin biosynthesis is negatively regulated by AbrB, a DNA-binding protein, by directly binding to the upstream region of pmxA [ 11 ]. (hindawi.com)
The laboratory has equipments such as a Biosafety Level 2 (BSL 2) cabinet, incubator shaker and electroporator to support upstream experiments such as bacterial isolation and DNA extraction. (ukm.my)

This happened using four different commercial extraction kits. (the-scientist.com)
The assumption has been they are sterile," said Walker, adding that, to be fair, DNA extraction kits aren't marketed as such. (the-scientist.com)
The loss of DNA does not seem to be issue when commercial DNA extraction kits are used. (hawaii.edu)

Our data suggested that medium supplemented with blood could increase the diversity of the bacterial community. (springeropen.com)

These results indicate that the concentration necessary for dependable sequencing is around 10,000 copies of target DNA per microliter. (harvard.edu)
Results: From an initial filter analysis, both the SKC PVC and SKC gelatin filters were selected for use in this project based on low-background DNA content, ease of use and cost. (cdc.gov)
From the obtained results, the Quick-DNA HMW MagBead Kit (Zymo Research) was selected as producing the best yield of pure HMW DNA. (altmetric.com)
Results from analytical studies showed that the Xpert MTB/RIF assay has analytic sensitivity of five genome copies of purified DNA, and 131 cfu/ml of M. tuberculosis spiked into sputum. (bvsalud.org)

Anatomy22

Organisms7

Diseases16

Chemicals and Drugs73

Analytical, Diagnostic and Therapeutic Techniques and Equipment86

Psychiatry and Psychology1

Phenomena and Processes24

Disciplines and Occupations8

Anthropology, Education, Sociology and Social Phenomena3

Technology, Industry, Agriculture12

Information Science15

Health Care17

Strains9

Bacteria15

Reagent3

Purification13

Species11

Lysis4

Phenol-chloroform2

Composition6

Microbiota3

Taxa1

Supported bacterial growth1

Genomic DNA1

Microbiome2

Contamination7

Amplification6

Genes2

Communities8

Numbers of bacterial1

Samples17

Cultures2

Nucleic acid3

Populations1

Agar1

Ambient Temperature1

Protocol5

Microbial DNA3

Commensal1

Inhibitors4

QIAGEN4

Strain1

Amplicon2

Inhibition1

Methods3

RDNA3

Assemblages1

Cystic fibrosis1

Downstream applications1

Protocols1

Purify2

Fungal2

Disruption1

Quantity and purity1

Detection3

Interactions1

Metabolic potential1

Deoxyribonucleic1

Saliva1

Antibacterial1

Method3

Detergent1

Upstream2

Kits3

Diversity1

Results4