Loading...
  • extract
  • By adding some protease inhibitors and by modifying the concentration of certain components in the extraction buffer we were able to obtain an extract with a HK activity stable for at least 72 h after extraction by preventing degradation. (umontreal.ca)
  • This new extraction method was able to extract chitosan completely from fungal cell wall, and had a higher recovery compared to the acetic acid extraction process [ 7 ]. (pubmedcentralcanada.ca)
  • First part (steps A1-A3) of the extraction provides a crude extract of Rubisco that is sufficient for carboxylation assays to measure the Michaelis constant for CO 2 (K C ) and the catalytic turnover rate ( k cat c ). (bio-protocol.org)
  • Gilson
  • From our comprehensive range of solid phase extraction (SPE) instruments and cartridges to our novel magnetic bead platform for isolating protein complexes, Gilson has the extraction solution you need for consistent, reproducible results. (gilson.com)
  • Buffer
  • DNA fragments selectively absorbed in the spin column after incubation at 60C for gel extraction or at room temperature for PCR product with equal volume of binding buffer. (bio-medicine.org)
  • detection
  • In this study, we present a new method that allows the reduction of costs and time required for the entire process of diagnosis, enabling the detection of TrV without resorting to RNA extraction, and without running the risk of degrading the RNA during the extraction, purification and storage process. (hindawi.com)
  • Process
  • A process for the extraction and purification of the diterpene alcohol Plaunotol from the leaves of the medicinal plant, Croton sublyratus Kurz. (freepatentsonline.com)
  • 6. A process as claimed in claim 1 wherein the alcoholic extraction of the dried ground leaves is carried out at a temperature of from 60° to 80° C. for a period of 60 to 120 minutes in an agitated extracting vessel. (freepatentsonline.com)
  • This invention relates to a process for the extraction and purification of an acyclic diterpene alcohol called Plaunotol, of the following formula: ##STR1## from the Thai medicinal plant "Plau-noi", identified as Croton sublyratus Kurz. (freepatentsonline.com)
  • It probably means that a pH-increasing step is also needed in the sulfuric-acid extraction process for production of fungal chitosan. (pubmedcentralcanada.ca)
  • Whether you are looking for discovery scale systems or transitioning from pilot to process scale, we can help you configure a purification system to meet your requirements. (gilson.com)
  • organic
  • Due to differences in cell structure and organic composition between organisms, the method for the purification of viable Rubisco enzyme needs to be continually optimized. (bio-protocol.org)
  • method
  • The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus. (hindawi.com)
  • This extraction method provided a simple and cost effective route to obtain almost pure stevioside and rebaudioside-A. (ijpsonline.com)
  • On the other hand, a method has recently been developed for extraction of chitosan from zygomycetes cell wall, which is based on the temperature-dependent solubility of chitosan in dilute sulfuric acid solutions. (pubmedcentralcanada.ca)
  • The method is similar to Whitney and Sharwood (2007), used for the purification of Rubisco overexpressed in E. coli , in that a French press is used to mechanically rupture cells. (bio-protocol.org)
  • solutions
  • The solubility of chitosan in acidic solutions such as acetic acid offers the possibility for its purification from the fungal cell wall [ 3 - 7 ]. (pubmedcentralcanada.ca)
  • The extraction and precipitation of pure shellfish chitosan with dilute-sulfuric acid may be accompanied by partial depolymerization of chitosan as well as reaction of chitosan with sulfate ions, such that by cooling the solution, the product precipitates and becomes insoluble in acetic acid solutions [ 8 ]. (pubmedcentralcanada.ca)
  • efficient
  • This study focuses on the extraction and isolation of a natural anti-inflammatory phycocyanin and its nanoformulation in innovative and efficient vesicular carriers able to improve its delivery to the skin. (csic.es)
  • temperature
  • A Box-Behnken factorial design (BBD) was used to investigate the effects of three independent variables, namely extraction temperature (X 1 ), extraction time (X 2 ) and ratio of water to raw material (X 3 ). (mdpi.com)