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Focal AdhesionsStress FibersFocal Adhesion Protein-Tyrosine KinasesFocal Adhesion Kinase 1Cell AdhesionPaxillinCell Adhesion MoleculesVinculinActinsCytoskeletonCell MovementFocal Adhesion Kinase 2ZyxinProtein-Tyrosine KinasesrhoA GTP-Binding ProteinCytoskeletal ProteinsIntegrinsActininTissue AdhesionsNerve FibersPhosphorylationrho GTP-Binding ProteinsCells, CulturedCrk-Associated Substrate ProteinSignal TransductionIntercellular Adhesion Molecule-1rho-Associated KinasesFibronectinsActin CytoskeletonTalinFibroblastsPhosphoproteinsAntigens, CD29Muscle Fibers, SkeletalMicrofilament ProteinsExtracellular MatrixVascular Cell Adhesion Molecule-1Microscopy, FluorescenceTyrosineDietary FiberCell LineCell ShapePseudopodia3T3 Cellssrc-Family KinasesStress, MechanicalMyosin Type IIMyosin Light ChainsBacterial AdhesionRetinoblastoma-Like Protein p130Protein BindingFluorescent Antibody TechniqueEnzyme ActivationEndothelium, VascularLIM Domain ProteinsTransfectionMechanotransduction, Cellularrac1 GTP-Binding ProteinSyndecan-4Cell SizeEndothelial CellsCytochalasin Drac GTP-Binding ProteinsMolecular Sequence DataMineral FibersGTPase-Activating ProteinsCell Surface ExtensionsCadherinscdc42 GTP-Binding ProteinAmino Acid SequencePhosphotyrosineCell Line, TumorRecombinant Fusion ProteinsProtein Structure, TertiaryNIH 3T3 CellsModels, BiologicalLysophospholipidsSwiss 3T3 CellsrhoB GTP-Binding ProteinProto-Oncogene Proteins pp60(c-src)Integrin alpha5beta1Muscle Fibers, Fast-TwitchMuscle Fibers, Slow-TwitchCell-Matrix JunctionsIntracellular Signaling Peptides and ProteinsBlotting, WesternTime FactorsProtein-Serine-Threonine KinasesAmidesBotulinum ToxinsActomyosinCotton FiberContractile ProteinsEpithelial CellsRNA, Small InterferingAdaptor Proteins, Signal TransducingGuanine Nucleotide Exchange FactorsMicroscopy, ConfocalReceptors, VitronectinNeural Cell Adhesion Molecules
CytoskeletonProteinsPhosphorylationVitreous fibersMigrationEndothelial cellsCollagenDorsalRegulationPlasma membraneCellsCytoskeletalVentralSignals2020FormationDynamicsCellularCompositionSpatialOxidative stressFibroblastIntracellularInhibitGTPaseChrysotileSyntheticDegradationStromalIntegrinsVinculinStructuresAsbestosElastinTyrosineTissueInhalationMechanismsContractionStiffnessNewlyDifferentialOccurResultsExcessive
Cytoskeleton2
Proteins10
Phosphorylation3
  • Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. (assaygenie.com)
  • Here, we find that enhanced phosphorylation of focal adhesion kinase (FAK) is associated with scratch-exacerbated AD. (nature.com)
  • Conversely, the RNA interference-mediated depletion of CIB1 increases cell migration and reduces normal adhesion-induced PAK1 activation and cofilin phosphorylation. (rupress.org)
Vitreous fibers2
  • In situ microscopic analysis of asbestos and synthetic vitreous fibers retained in hamster lungs following inhalation. (cdc.gov)
  • A relationship between the extent of water adsorption and biopersistence is proposed for vitreous fibers. (nih.gov)
Migration8
  • Rho-GTPases are involved in regulating cell morphology (through cytoskeletal organization) and migration (through focal adhesion formation). (wikipedia.org)
  • 13. Dynamics of Actin Stress Fibers and Focal Adhesions during Slow Migration in Swiss 3T3 Fibroblasts: Intracellular Mechanism of Cell Turning. (nih.gov)
  • Collectively, our results strongly suggest that intact keratin IF networks regulate mechanotransduction through a Rho signaling pathway upstream of cell-ECM adhesion formation and organized cell migration. (biologists.com)
  • Participates in signaling pathways that control a diverse spectrum of biological activities including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. (drugbank.com)
  • ROCK is involved with subsequent stabilization and maturation of adhesions, which supports migration in the direction of the attractive signal. (mechanobio.info)
  • SOX10 or high SOX9 expression regulates melanoma mesenchymal migration through the NEDD9-mediated focal adhesion dynamics and Rho GTPase signaling. (biomedcentral.com)
  • Together, these results demonstrate that endogenous CIB1 is required for regulated adhesion-induced PAK1 activation and preferentially induces a PAK1-dependent pathway that can negatively regulate cell migration. (rupress.org)
  • Upon adhesion to ECM, cytoskeletal rearrangements occur that lead to cell spreading, actin turnover, and cell migration. (rupress.org)
Endothelial cells2
  • Regions of focal detachment and loss of Schlemm's canal endothelial cells and trabecular meshwork extracellular matrix have been observed in tissues treated with the topical PG, latanoprost. (medscape.com)
  • Further, we measured microvascular density and underlying TRPV4 mechanotransduction mechanisms using human microvascular endothelial cells, extracellular matrix gels of varying stiffness, unbiased RNA sequencing, small interfering RNA, Western blot, quantitative-PCR, and confocal immunofluorescence techniques. (bvsalud.org)
Collagen9
  • Differential dynamics of early stages of platelet adhesion and spreading on collagen IV- and fibrinogen-coated surfaces. (weizmann.ac.il)
  • Previous reports in the literature have shown that collagen-glycosaminoglycan (GAG) copolymers with tissue-specific pore diameters and degradation rates, serving as analogs of extracellular matrix (ECM), favor the processes of regeneration in dermis and peripheral nerve. (mrs.org)
  • Collagen-glycosaminoglycan (GAG) copolymers, serving as analogs of extracellular matrix, have been used successfully as implants to facilitate regeneration of dermis and peripheral nerve. (mrs.org)
  • The right panel is a schematic representation of the extracellular matrix of the alveolar septal wall with various components including amorphous elastin (El), wavy collagen (C), complex proteoglycans (PG), basement membrane (BM) and fibroblast cells (F). (Drawing by E. Bartolák‐Suki). (comprehensivephysiology.com)
  • It can be seen that some fibers are comprised of almost exclusively type I or type III collagen (black arrows), whereas at several locations, the two collagen types also appear to co‐localize suggesting that they mix and form composite fibers (red arrow) where the color is intermediate between blue and brown. (comprehensivephysiology.com)
  • Stress‐strain curve of collagen in tendon. (comprehensivephysiology.com)
  • Arrows denote collagen fibers and the white regions are elastin. (comprehensivephysiology.com)
  • 3D collagen architecture regulates cell adhesion through degradability, thereby controlling metabolic and oxidative stress. (ucsd.edu)
  • Crowding tunes 3D collagen fibers and reveals matrix regulation of cancer cell morphogenesis. (ucsd.edu)
Dorsal2
Regulation2
  • 5. Molecular mechanisms underlying the force-dependent regulation of actin-to-ECM linkage at the focal adhesions. (nih.gov)
  • Biomechanical regulation of focal adhesion and invadopodia formation. (weizmann.ac.il)
Plasma membrane2
  • This interplay between actin arcs and focal adhesions in turn creates an anchored, stiff substrate against which the polymerizing actin filaments extend the plasma membrane. (nih.gov)
  • Following Burnstock's pioneering work on the role of ATP-sensitive P2 purinoceptors, its metabolite adenosine soon became recognized as an extracellular signalling molecule through the activation of plasma membrane-bound P1 receptors family that are expressed in every organ systems in the body. (frontiersin.org)
Cells15
  • DLC1 is localized to focal adhesions located at the periphery of cells. (wikipedia.org)
  • In addition, cells of the DLC1-/- embryos had few long actin fibers (indicating that their cytoskeletal organization was impaired) and fewer focal adhesions than those of normal DLC1 expressing cells. (wikipedia.org)
  • DLC1 is also involved in the formation of focal adhesions, so loss of DLC1 leads to reduced cell adhesion and increased metastatic potential of cells. (wikipedia.org)
  • Cancer cells sense and respond to extracellular matrix stiffening through the process of mechanotransduction. (nih.gov)
  • In this work, we aimed to culture and subculture primary alveolar type II cells on extracellular matrix lung-derived hydrogels to assess their suitability for phenotype maintenance. (ibecbarcelona.eu)
  • Moreover, cells cultured on a plate showed the active form of the YAP protein and the formation of stress fibers and focal adhesions. (ibecbarcelona.eu)
  • This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. (assaygenie.com)
  • Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. (assaygenie.com)
  • 14 - 17 ] Mammalian cells can sense and interact with the extracellular microenvironment at the nanometer scale despite their micrometer dimensions. (ntno.org)
  • Here, we demonstrate that, in contrast to keratin-deficient keratinocytes, cells expressing K14 R125C , a mutation that causes severe EBS, generate lower traction forces, accompanied by immature focal adhesions with an altered cellular distribution. (biologists.com)
  • She described how Dylan Burnette , the leading postdoctoral researcher on the project, studied the spatial relationship between actin and the focal adhesion protein zyxin and found a correlation between the contracting actin arcs and focal adhesion points (actin-rich structures that enable cells to adhere to the extracellular matrix). (nih.gov)
  • style of artificial extracellular matrix with murine mesenchymal stem cells [8]. (tech-strategy.org)
  • Exosomes correspond to a class of extracellular nanovesicles, produced by a broad range of human somatic and cancerous cells. (biomedcentral.com)
  • Exosomes, a general term for a particular class of nano-extracellular vesicles are produced by various stromal and transformed cells in the tumor microenvironment (TME) [ 13 , 14 ]. (biomedcentral.com)
  • 1998. Activation of alveolar macrophages and peripheral red blood cells in rats exposed to fibers/particles. (cdc.gov)
Cytoskeletal2
  • 20. Integrin-mediated cell adhesion: the cytoskeletal connection. (nih.gov)
  • 11 - 13 ] Cell-material interactions can direct cell shape and cytoskeletal organization, which in turn control biological processes, such as cell adhesion, growth, differentiation, and apoptosis. (ntno.org)
Ventral1
  • 12. Adhesive F-actin waves: a novel integrin-mediated adhesion complex coupled to ventral actin polymerization. (nih.gov)
Signals4
  • During neural development, highly motile structures on the developing neurites, called growth cones, are guided by signals from the extracellular environment. (mechanobio.info)
  • Most known attractive signals act as chemoattractants, often generating the formation of adhesion molecules within the growth cone to promote selective extension of the filopodia towards the cue, whilst ensuring the formation of filopodia or lamellipodia is decreased in other directions. (mechanobio.info)
  • Semaphorins are a wide family of extracellular regulatory signals with a multifaceted role in cell-cell communication. (biomed.news)
  • 1.5 Interactions of ARSB withBMP4 and Wnt Within this survey we present mechanisms that integrate extracellular signals with intracellular transcriptional events as necessary for developmental functions. (tech-strategy.org)
20201
  • Only in 1972, Geoff Burnstock (born: May 10, 1929, died: June 2, 2020) coined the term purinergic signalling referring to the extracellular effects of adenosine 5′-triphosphate (ATP). (frontiersin.org)
Formation3
  • 16. Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation. (nih.gov)
  • Notably, RhoA activity is downregulated in human EBS keratinocytes, and Rho activation rescues stiffness-dependent cell-extracellular matrix (ECM) adhesion formation of EBS keratinocytes. (biologists.com)
  • Furthermore, we revealed enhanced focal adhesion formation and osteogenic differentiation of MSCs cultured on positively charged protein fibers. (nature.com)
Dynamics2
  • 11. Control of high affinity interactions in the talin C terminus: how talin domains coordinate protein dynamics in cell adhesions. (nih.gov)
  • 19. Talin Autoinhibition Regulates Cell-ECM Adhesion Dynamics and Wound Healing In Vivo. (nih.gov)
Cellular3
  • Non-receptor protein tyrosine kinase which is activated following engagement of many different classes of cellular receptors including immune response receptors, integrins and other adhesion receptors, receptor protein tyrosine kinases, G protein-coupled receptors as well as cytokine receptors. (drugbank.com)
  • The relationship between stress to the ECM and its effects on development of tendinosis at the cellular level is introduced. (journalofprolotherapy.com)
  • Cellular effects of mineral fibers. (cdc.gov)
Composition3
Spatial2
  • 18. Molecular mechanism of vinculin activation and nanoscale spatial organization in focal adhesions. (nih.gov)
  • These applications require the engineering of a programmable two- or three-dimensional biomaterial with micro and/or nanometer features that could mimic certain aspects of the extracellular environment such as the spatial arrangement and density of ligands. (ntno.org)
Oxidative stress3
  • Although oxidative stress and mechanical scratching can aggravate AD inflammation, treatment targeting scratching is often overlooked, and the efficiency of mechano-chemically synergistic therapy remains unclear. (nature.com)
  • We then develop a multifunctional hydrogel dressing that integrates oxidative stress modulation with FAK inhibition to synergistically treat AD. (nature.com)
  • Oxidative stress, as an important factor in many inflammatory diseases, is increasingly recognized to be associated with the development of AD 22 . (nature.com)
Fibroblast1
  • 2. Early molecular events in the assembly of the focal adhesion-stress fiber complex during fibroblast spreading. (nih.gov)
Intracellular2
  • Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. (assaygenie.com)
  • Extracellular and intracellular indicators could be integrated through modulation of ARSB activity by air and the linked adjustments in chondroitin 4-sulfation [15]. (tech-strategy.org)
Inhibit1
  • It acts as a tumor suppressor gene to inhibit cell growth and proliferation as well as induce apoptosis when a cell is under stress. (wikipedia.org)
GTPase1
  • Receptor-binding transiently activates Cdc42/Rac1 GTPase and subsequent recruitment of adhesion molecules and components of the actin polymerizing module (e.g. (mechanobio.info)
Chrysotile1
  • Modifications of the surface taking place in vivo are described for ferruginous bodies and for the progressive comminution of chrysotile asbestos fibers. (nih.gov)
Synthetic1
  • For this purpose, we developed a fibrous scaffold resembling the bone/bone marrow extracellular matrix (ECM) based on protein without addition of synthetic polymers. (nature.com)
Degradation1
  • [ 21 ] PGs may directly control the resistance to trabecular outflow by initiating the degradation of the extracellular matrix via their effects on matrix metalloproteinases (MMPs) expressed in the trabecular meshwork. (medscape.com)
Stromal1
  • The corneal stroma is composed of keratocytes (2%-3% of the total stromal volume) [ 6 ], extracellular matrix (ECM) molecules and stromal nerves. (molvis.org)
Integrins1
Vinculin1
  • 8. Vinculin-actin interaction couples actin retrograde flow to focal adhesions, but is dispensable for focal adhesion growth. (nih.gov)
Structures1
  • Biotensegrity or hierarchical tensegrity can explain how prestressed structures function in animals to transfer the stresses created by gravity, movement, digestion and emotional factors to the extracellular matrix (ECM). (journalofprolotherapy.com)
Asbestos3
  • Iron in amphibole asbestos is implicated in the pathogenicity of inhaled fibers. (nih.gov)
  • We found that chelators can affect the amount of iron at the surface of the asbestos fibers and its valence, and that they can modify the chemical reactivity of these surfaces. (nih.gov)
  • Exposure to non-asbestos refractory materials: Corrections to fiber counts for comparable risk assessment. (cdc.gov)
Elastin2
Tyrosine1
  • The relatively unstructured and unconserved SR region (amino acids 86-638) contains a focal adhesion targeting (FAT) domain, including a tyrosine residue at position 442, which interacts with SH2 domains of tensin1 and cten. (wikipedia.org)
Tissue5
  • Fibrosis is an irreversible, debilitating condition marked by the excessive production of extracellular matrix and tissue scarring that eventually results in organ failure and disease. (bvsalud.org)
  • The extracellular matrix (ECM) is an intricate network composed of an array of multidomain macromolecules organized in a cell/tissue-specific manner. (uncommondescent.com)
  • The frame also extends to form the periosteum which supplies vascular structure to the bone, which can be considered as just another connective tissue or extracellular matrix [ 12 ]. (biomedcentral.com)
  • A) Stress‐strain curves of parenchymal tissue strips from a normal rat and a rat that had been treated with elastase‐mimicking pulmonary emphysema. (comprehensivephysiology.com)
  • Evidence includes the observation that iron chelators can suppress fiber-induced tissue damage. (nih.gov)
Inhalation1
  • Continuous redox cycling of iron, with consequent activation-reactivation of the surface sites releasing free radicals, could account for the long-term pathogenicity caused by the inhalation of iron-containing fibers. (nih.gov)
Mechanisms1
  • The purpose of this study was to explore possible mechanisms for the reduction of fiber toxicity by iron chelator treatments. (nih.gov)
Contraction1
  • The absence of this crucial structural protein leads to sarcolemmal fragility, resulting in muscle fiber damage during contraction. (biomed.news)
Stiffness1
  • Tensegrity enables the immediate response to changes in force by altering the stiffness in direct proportion to the applied mechanical stress. (journalofprolotherapy.com)
Newly1
  • Moving actin arcs slowed down near newly forming focal adhesions, likened to "feet" at the point of contact between the cell and a surface. (nih.gov)
Differential1
Occur2
  • Although cancer is a genetic disease, physical changes such as stiffening of the extracellular matrix also commonly occur in cancer. (nih.gov)
  • This is believed to occur via the diminished production of fiber-associated reactive oxygen species. (nih.gov)
Results1
  • The results have been compared with the ability of the fibers to produce free radicals and decompose hydrogen peroxide in a cell-free system in vitro. (nih.gov)
Excessive1