• Bone marrow-derived mesenchymal stem cells (MSC) have been promoted for multiple therapeutic applications. (nih.gov)
  • Mesenchymal stem/stromal cells (MSCs) obtained from pluripotent stem cells (PSCs) constitute an interesting alternative to classical MSCs in regenerative medicine. (unlp.edu.ar)
  • This report addresses the issue of optimizing extracellular matrix protein density required to support osteogenic lineage differentiation of mesenchymal stem cells (MSCs) by culturing MSCs on surface-bound density gradients of immobilized collagen type I (COL1) and osteopontin (OPN). (monash.edu)
  • Mesenchymal stem cells (MSCs) are multipotent cells with regenerative and immunomodulatory properties. (frontiersin.org)
  • Human mesenchymal stem cells (HMSCs) are multipotent somatic stem cells that can be isolated from a variety of tissues such as the bone marrow, adipose tissue, and dental pulp. (frontiersin.org)
  • Researchers from the West Virginia University School of Medicine demonstrate that osteoblasts-derived (Os-Exo) and adipocytes-derived (Ad-Exo) exosomes contain instructive factors that regulate the lineage specification of human mesenchymal stem cells (hMSCs). (exosome-rna.com)
  • Merckx G, Hosseinkhani B, Kuypers S, Deville S, Irobi J, Nelissen I, Michiels L, Lambrichts I, Bronckaers A. (2020) Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles. (uhasselt.be)
  • Mesenchymal stem cell medium was used in the control groups. (scielo.br)
  • Anatomical region-specific enhancement of 3-dimensional chondrogenic differentiation of human mesenchymal stem cells by soluble meniscus extracellular matrix. (chop.edu)
  • The effects of erythropoietin (EPO) on the behaviors of bone marrow mesenchymal stem cells (BMSCs) subjected to mechanical stretch remain unclear. (hindawi.com)
  • Notably, it promotes the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) [ 15 - 17 ]. (hindawi.com)
  • Mesenchymal stem/stromal cells (MSC) are the ideal source to study fat formation as they are the progenitors of adipocytes. (mdpi.com)
  • Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
  • Mesenchymal stem/progenitor cells (MSC/MPC) are found in many tissues and fluids including bone marrow, adipose tissues, muscle, synovial membranes, synovial fluid, and blood. (scirp.org)
  • Hart, D. (2014) Is Adipocyte Differentiation the Default Lineage for Mesenchymal Stem/Progenitor Cells after Loss of Mechanical Loading? (scirp.org)
  • Hart, D.A. (2014) Why Mesenchymal Stem Cell/Progenitor Cell Heterogeneity in Specific Environments? (scirp.org)
  • Wu, Y. and Zhao, R.C. (2012) The Role of Chemokines in Mesenchymal Stem Cell Homing to the Myocardium. (scirp.org)
  • Li, L. and Jiang, J. (2011) Regulatory Factors of Mesenchymal Stem Cell Migration into Injured Tissues and Their Signal Transduction Mechanisms. (scirp.org)
  • Fehrer, C. and Lepperdinger, G. (2005) Mesenchymal Stem Cell Aging. (scirp.org)
  • Batsali, A.K., Kastrinaki, M.C., Papadaki, H.A. and Pontikoglou, C. (2013) Mesenchymal Stem Cells Derived from Wharton's Jelly of the Umbilical Cord: Biological Properties and Emerging Clinical Applications. (scirp.org)
  • Human dental pulp-derived mesenchymal stem cells (hDP-MSCs), which include human dental pulp stem cells (hDPSCs) and stem cells from human exfoliated deciduous teeth (SHEDs), are promising cell sources for regenerative therapies. (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) are stromal cells that have two key features, self-renewal and the ability to differentiate along different lineages [ 1 ]. (biomedcentral.com)
  • Human liver organoids are formed from the assembly of hepatocyte-like cells differentiated from iPS cells, human umbilical vascular endothelial cells and mesenchymal stem cells ( 9 ). (spandidos-publications.com)
  • HLSCs do not express hematopoietic stem cell markers, whereas they express several mesenchymal markers and display multiple differentiating abilities ( 22 ). (spandidos-publications.com)
  • The bone morrow contains several stem cell types including hematopoetic stem cells, endothelial progenitor cells, mesenchymal (stromal) stem cells and multipotent adult progenitor cells. (escardio.org)
  • Mesenchymal stem cells (MSCs) possess great therapeutic potential. (hindawi.com)
  • The differentiation of mesenchymal stem cells (MSCs) into multiple cell lineages can be exploited as an attractive strategy for cell-based therapy and regenerative medicine [ 1 ]. (hindawi.com)
  • These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)
  • Many beneficial effects of MSCs are paracrine, dependent on extracellular vesicles (EVs). (nih.gov)
  • Among their many mechanisms of action, MSC extracellular vesicles (EVs) are a potential suitable substitute for MSCs in future cell-free-based therapeutic approaches. (unlp.edu.ar)
  • Several aspects of MSC function have been attributed to the paracrine effects of MSC derived extracellular vesicles (EVs). (frontiersin.org)
  • Extracellular vesicles (EVs)/exosomes can satisfy these criteria ( Marcus and Leonard, 2013 ). (frontiersin.org)
  • Hereby we focus on the use of extracellular vesicles derived from dental pulp stem cells, a project with dr. (uhasselt.be)
  • Evidence indicates that Alix, an accessory protein of the endosomal sorting complex required for transport (ESCRT), is involved in the biogenesis of extracellular vesicles (EVs). (spandidos-publications.com)
  • It has been recognized that under physiological and pathological conditions, cells may release vesicles that may be instrumental in intercellular communication as they carry biologically active molecules ( 1 - 4 ). (spandidos-publications.com)
  • The non-apoptotic vesicles have been classified based on their biogenesis into exosomes derived from membranes of the endosomal compartment and microvesicles that are formed from the budding of a portion of the cell plasma membrane ( 5 , 6 ). (spandidos-publications.com)
  • Given the heterogeneity of the released vesicles and the overlapping features of molecules carried by exosomes and microvesicles, it has been suggested that they be collectively termed extracellular vesicles (EVs) ( 7 ). (spandidos-publications.com)
  • Aliotta et al ( 15 ) demonstrated that lung-derived vesicles carried RNA to marrow cells and altered the phenotype of these cells both genetically and functionally. (spandidos-publications.com)
  • Vesicles derived from adult human liver stem-like cells (HLSCs) have also been shown to contain ribonucleoproteins and different RNA species involved in hepatic regeneration ( 20 ). (spandidos-publications.com)
  • Recent studies have shown that extracellular vesicles (EVs) can be exploited by tumors to assist in cancer cell growth, proliferation, migration, invasion and metastasis. (springer.com)
  • Anand S, Samuel M, Kumar S, Mathivanan S (2019) Ticket to a bubble ride: cargo sorting into exosomes and extracellular vesicles. (springer.com)
  • Becker A, Thakur BK, Weiss JM, Kim HS, Peinado H, Lyden D (2016) Extracellular vesicles in cancer: cell-to-cell mediators of metastasis. (springer.com)
  • Catalano M, O'Driscoll L (2020) Inhibiting extracellular vesicles formation and release: a review of EV inhibitors. (springer.com)
  • Journal of Extracellular Vesicles 9(1):1703244. (springer.com)
  • Mechanistically, CTRP3 elicited a transcriptomic pattern that stimulates abnormal differentiation of tendon stem/progenitor cells and ectopic chondrification as an effect linked to activation of Akt signaling. (ewha.ac.kr)
  • They have a higher odontogenic differentiation capacity compared to other cell groups due to their content of specific progenitor cells. (scielo.br)
  • Dynamic Compressive Loading Improves Cartilage Repair in an in vitro Model of Microfracture: Comparison of Two Mechanical Loading Regimens on Simulated Microfracture Based on Fibrin Gel Scaffolds Encapsulating Connective Tissue Progenitor Cells. (chop.edu)
  • Erythropoietin is a glycoprotein hormone that stimulates red blood cell (RBC) production in bone marrow via binding to the cell-surface receptor on hematopoietic progenitor cells, and it has been widely used for treating anemia [ 13 ]. (hindawi.com)
  • Ando, W., Heard, B., Nakamura, N., Frank, C.B. and Hart, D.A. (2012) Ovine Synovial Membrane-Derived Mescenchymal Progenitor Cells Retain the Phenotype of the Original Tissue That Was Exposed to in Vivo Inflammation: Evidence for a Suppressed Chondrogenic Differentiation Potential of the Cells. (scirp.org)
  • Although there is no definitive evidence, combinations of progenitor cells seem to be more benefical than specific stem cell type for cardiac repair (3). (escardio.org)
  • Interestingly, contradictory data exist for transformation of bone marrow derived progenitor cells (BMPC) into new cardiomyocytes and alternative mechanisms as enhanced neovascularization, enhanced scar tissue formation due to augmented inflammatory response and decreased apoptosis have been suggested for the benefical effects of these cells on myocardial function after myocardial infarction (4). (escardio.org)
  • Downloaded from periodic thymic colonization by progenitor cells guarantees stage. (lu.se)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
  • Overall, the results presented in this study show a common mechanism of endocytosis for MSC EVs across different cell types and the feasibility to generate functionally enhanced EVs by modifications to parental MSCs. (frontiersin.org)
  • We then sought to understand the role of 21 mobility in modulating osteogenic differentiation of human MSCs in the presence and absence of stimulation with BMP-2. (gla.ac.uk)
  • This systematic review aims to provide a panel of miRNAs that regulate the differentiation of hDP-MSCs including hDPSCs and SHEDs. (biomedcentral.com)
  • Experimental studies assessing the promotive/suppressive effect of miRNAs on the differentiation of hDP-MSCs and studies evaluating changes to the expression of miRNAs during the differentiation of hDP-MSCs were included. (biomedcentral.com)
  • Thirteen miRNAs promoted and 17 suppressed odontogenic/osteogenic differentiation of hDP-MSCs. (biomedcentral.com)
  • hsa-miR-140-5p, hsa-miR-218 and hsa-miR-143 were more frequently reported suppressing the odontogenic/osteogenic differentiation of hDP-MSCs. (biomedcentral.com)
  • KEGG pathway analysis revealed MAPK, PI3K-Akt and FoxO as key signaling pathways involved in the odontogenic/osteogenic differentiation of hDP-MSCs. (biomedcentral.com)
  • The findings of this systematic review support the potential application of the specific miRNAs to regulate the directed differentiation of hDP-MSCs in the field of regenerative therapies. (biomedcentral.com)
  • hDP-MSCs includes human dental pulp stem cells (hDPSCs) and stem cells from human exfoliated deciduous teeth (SHEDs) which are isolated from the pulp tissue of permanent and deciduous teeth, respectively. (biomedcentral.com)
  • In addition, their osteogenic differentiation potential was increased, and genes involved in cell adhesion, FGF-2 signalling, cell cycle, stemness, cell differentiation, and cell proliferation were upregulated, compared to that of the MSCs cultured on uncoated plates. (hindawi.com)
  • The clinical application of MSCs to tissue engineering has been introduced due to their numerous advantages including high expansion potential and extensive differentiation potential [ 6 , 7 ]. (hindawi.com)
  • However, MSCs need to be expanded in vitro in order to obtain sufficient cells for clinical trials since they are extremely rare in various tissues. (hindawi.com)
  • Unlike embryonic stem cells, adult stem cells (MSCs) have a limited lifespan and stop proliferating during in vitro culture due to replicative senescence [ 8 ]. (hindawi.com)
  • MSCs naturally reside in a specialized niche in vivo , which mainly consists of the extracellular matrix (ECM). (hindawi.com)
  • Our approach is to first understand how hESC interact with extracellular matrix materials by analyzing candidate adhesive substrate proteins and integrin receptors that mediate attachment, survival, proliferation and differentiation. (ca.gov)
  • Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
  • However, details regarding the regulation of MSC/MPC proliferation and differentiation status are still unclear and it is likely that regulation involves both biological and mechanical influences in the different environments. (scirp.org)
  • We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
  • Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
  • Second, altering the MSC state by inducing differentiation into multiple lineages did not affect the exosomal properties or endocytosis but triggered the expression of lineage-specific genes and proteins in vitro and in vivo respectively. (frontiersin.org)
  • In this regard, tissue engineers have sought to leverage native mechanotransduction pathways to enhance in vitro stem cell. (biomedcentral.com)
  • Tissue engineering has developed many paradigms and techniques on how to best integrate cells and extracellular matrix to create in vitro structures that replicate native tissue. (biomedcentral.com)
  • The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. (scielo.br)
  • In vitro repair of meniscus radial tear using hydrogels seeded with adipose stem cells and TGF-β3. (chop.edu)
  • The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
  • These polymeric materials organise fibronectin (FN), an important protein of the extracellular matrix, and sequester rhBMP-2 in synergy with the integrin binding region to direct stem cell differentiation in vitro. (interface-online.org.uk)
  • At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
  • Next, we compared both datasets and found that while iPSC EVs enclose proteins that modulate RNA and microRNA stability and protein sorting, PD-MSC EVs are rich in proteins that organize extracellular matrix, regulate locomotion, and influence cell-substrate adhesion. (unlp.edu.ar)
  • Moreover, compared to their respective cells, iPSCs and iPSC EVs share a greater proportion of proteins, while the PD-MSC proteome appears to be more specific. (unlp.edu.ar)
  • b Cells were collected and heated in a narrow temperature range, with one sample incubated at 37 °C for expression measurements, protein aggregates were eliminated by ultracentrifugation and the soluble proteins were digested. (nature.com)
  • Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)
  • ECM of differentiated cells modulates phenotypic expression of stem cells, while their exosomes contain phenotype specific instructive factors (miRNA, RNA and proteins) that control stem cell differentiation. (exosome-rna.com)
  • As a sorting receptor on the cell surface and on the endoplasmic reticulum-Golgi apparatus within the cell, sortilin is involved in the transport of a wide variety of intracellular proteins between the trans-Golgi network, endosome, lysosome, and secretory granules, as well as the plasma membrane. (wikipedia.org)
  • Bone morphogenetic proteins (BMP), known as active components of odontogenic differentiation and tooth development, have been used as a growth factor in an appropriate medium. (scielo.br)
  • First, we characterized the cell surface receptors for matrix proteins on stem cells and identified specific proteins that will support adhesion and growth of the cells. (ca.gov)
  • Second, using an interdisciplinary approach, we developed a novel method to screen peptides from these proteins for their ability to support adhesion and proliferation of the stem cells. (ca.gov)
  • Based on our characterization of cell surface receptors for matrix proteins on stem cells completed in the first year, we went on to test specific, purified proteins that will support adhesion and growth of the cells. (ca.gov)
  • Cells interact with the external environment via a layer of proteins and respond to not only biochemical cues but also physical properties including stiffness and topography of adjacent surfaces. (gla.ac.uk)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • Poly-L-lysine (PLL) of extracellular matrix proteins is a small natural homopolymer of the essential amino acid L-lysine that is used to coat culture substrates. (hindawi.com)
  • We found novel proteins were dysregulated under simulated microgravity, including CSC1-like protein, involved in the mechanotransduction of pressure signals, and PTPN11, SLC44A1 and MME which are involved in osteoblast differentiation pathways and which may become the focus of future translational projects. (lu.se)
  • The investigation of cell proteome highlighted how simulated microgravity affects a relatively low number of proteins compared to time and/or osteogenic factors and has allowed us to reconstruct a hypothetical pipeline for cell response to simulated microgravity. (lu.se)
  • Hard tissue formation in a porous HA/TCP ceramic scaffold loaded with stromal cells derived from dental pulp and bone marrow. (scielo.br)
  • In this work, for the first time, we adopt a multidisciplinary approach to characterize the morphological, biochemical, and molecular changes underlying the response of human bone marrow stromal cells to long-term simulated microgravity exposure during osteogenic differentiation. (lu.se)
  • We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. (nature.com)
  • To get a new insight into pluripotency, we employed a plurifaceted experimental design combining expression proteomics with the proteome-wide integral solubility alteration (PISA) assay 7 to compare pluripotent cells with their isogenic progenies and parental cells as well as with allogeneic cells. (nature.com)
  • Induced pluripotent stem cells (iPSC), generated by introduction of a few defined factors in a somatic cell, provide an ideal patient-specific source for disease modeling, drug discovery and cellular therapies. (stanford.edu)
  • Finally, we used this approach to encapsulate human Neural Stem Cells (hNSC) derived from human Induced Pluripotent Stem Cells (hIPSC), which were further differentiated into neurons within the capsules with negligible loss of viability. (rsc.org)
  • Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
  • Whilst differentiation of some lineage specific stem cells can be induced with a degree of certainty, a differentiation outcome of a population of pluripotent stem cells is less predictable. (justia.com)
  • The Simons Foundation Autism Research Initiative (SFARI) hosted a virtual workshop on March 14 and 18, 2022, to discuss the best practices for the development and use of induced pluripotent stem (iPS) cell models for autism research. (sfari.org)
  • Human induced pluripotent stem (iPS) cells can differentiate into somatic cells ( 1 ). (spandidos-publications.com)
  • There is growing interest in the functional roles of the extracellular matrix (ECM) in regulating the fate of pluripotent stem cells (PSCs). (bvsalud.org)
  • ES cells are pluripotent cells, which mean that they can give rise to all differentiated cell types derived from all three germ layers. (bvsalud.org)
  • There is a critical need for new technologies to facilitate growth and differentiation of human embryonic stem cells (hESC) using clinically acceptable, animal-free reagents. (ca.gov)
  • We propose to develop novel, well-defined, synthetic extracellular matrices for growth and differentiation of hESC. (ca.gov)
  • Crystal L. Abrams with her research poster "the effect of extracellular matrix protein laminin-1 on growth and differentiation of mouse embryonic stem cells" Crystal is a MEC RISE student. (cuny.edu)
  • The practice of dentistry is likely to be revolutionized by biological therapies based on growth and differentiation factors that accelerate and/or induce a natural biological regeneration. (bvsalud.org)
  • We are interested in understanding the role of the extracellular matrix in regulating stem cell self-renewal and differentiation, and how this regulation goes awry in cancer. (stanford.edu)
  • The role of the extracellular signal-regulated kinases 1/2 (ERK1/2) in the osteogenesis of BMSCs stimulated by mechanical stretch combined with 20 IU/ml EPO was examined by Western blot. (hindawi.com)
  • Notably, human cancerous epithelial cells exhibited increased levels of sortilin as compared to normal epithelial tissues. (wikipedia.org)
  • In addition, we examined whether these substrates will support differentiation of retinal pigmented epithelial cells, an important, clinically relevant cell type that is currently in clinical trials for eye disease. (ca.gov)
  • Lung epithelial cells are located on the surface of the internal parts of the lung, and they are responsible for gas exchange (i.e., breathing). (lu.se)
  • Previously, our research team had found that two molecules, or more specifically, co-transcriptional regulators, known as YAP and TAZ, are involved in the damage of the lung epithelial cells. (lu.se)
  • Beghein E, Devriese D, Van Hoey E, Gettemans J (2018) Cortactin and fascin-1 regulate extracellular vesicle release by controlling endosomal trafficking or invadopodia formation and function. (springer.com)
  • This gradient approach may facilitate the screening of an optimal biomolecule surface density on tissue-engineered scaffolds, implants, or tissue culture ware to obtain the desired cell response, and may generate opportunities for more cost-effective regenerative medicine. (monash.edu)
  • Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
  • Although MSC EVs show great promise for regenerative medicine applications, insights into their uptake mechanisms by different target cells and the ability to control MSC EV properties for defined function in vivo have remained elusive knowledge gaps. (frontiersin.org)
  • Besides stroke, my lab has also a main interest in using (dental) stem cells for tissue regeneration . (uhasselt.be)
  • Consequently, PLDL and PDL seemed to be promising scaffold candidates for odontogenic regeneration at least as HA-TCP, when they were applied with the DPSC induced for odontogenic differentiation. (scielo.br)
  • Although different stem cells, morphogens and scaffolds can be combined to simulate natural odontogenic regeneration, it is crucial to determine which options most closely replicate the characteristics of human dental tissues. (scielo.br)
  • Furthermore, muscle regeneration is affected during cancer cachexia due to impaired muscle stem cell function. (leibniz-gemeinschaft.de)
  • Our results show that Wnt7a has a dual function in skeletal muscle: it activates an important signaling pathway enhancing muscle mass and drives muscle stem cell expansion in skeletal muscle, which is necessary for regeneration", says Dr. Manuel Schmidt, postdoc in the research group. (leibniz-gemeinschaft.de)
  • Wnt7a leads to an increase in the stem cell population in the muscle, which supports muscle regeneration after surgery. (leibniz-gemeinschaft.de)
  • The idea of myocardial regeneration with stem cell transplantation after myocardial infarction receives tremendous interest. (escardio.org)
  • Following injury or disease, an organism undergoes a variety of (natural or induced) processes to direct cells, tissues and cellular processes towards healing and eventually regeneration. (interface-online.org.uk)
  • There are limited numbers of publications about ES cells in pulp regeneration, due to the restricted policies regarding ES cell research over the past few years. (bvsalud.org)
  • Microenvironmental milieu consists of two essential components, one being extracellular matrix (ECM) as the substratum, while the other being cell secreted exosomes and growth factors. (exosome-rna.com)
  • In addition, several miRNAs reported to have osteogenic and adipogenic differentiation potentials are also identified in these exosomes. (exosome-rna.com)
  • Kinetic and differentiation analyses indicate that both osteoblast and adipocyte exosomes augment ECM-mediated differentiation of hMSCs into the respective lineage. (exosome-rna.com)
  • The combination of osteoblast/adipocyte ECM and exosomes turned-on the lineage specific gene expressions at earlier time points of differentiation compared to the respective ECM or exosomes administered individually. (exosome-rna.com)
  • In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
  • An artificially bioengineered ECM provides an excellent model for studying the molecular mechanisms underlying self-renewal and differentiation of PSCs, without multiple unknown and variable factors associated with natural substrates. (bvsalud.org)
  • Organoids from human tooth showing epithelial stemness phenotype and differentiation potential. (uhasselt.be)
  • Deficiencies are highly variable with regard to symptoms, phenotype, genotype, severity, etc, because many cells and molecules are required for both natural and adaptive immunity . (lu.se)
  • Stem cells differentiate into bone-forming osteoblasts, and mechanical stimulation is involved in this p. (biomedcentral.com)
  • Furthermore, mechanical stretch may induce the differentiation of BMSCs into mature osteoblasts and enhance the deposition of the bone matrix [ 18 - 20 ]. (hindawi.com)
  • My research continues to focus on stem cells and osteoblasts and in particular 3D cell culture models and microenvironments. (nottingham.ac.uk)
  • This was also the case for myogenic cell differentiation, which was indiscriminately higher on polymers with extended fibronectin, however, cytoskeletal contractility was found to play an essential role in the myogenic differentiation of cells on these polymers in a mobility dependent manner. (gla.ac.uk)
  • At the workshop, leaders in the field, including several SFARI Investigators, discussed best practices for the generation and characterization of the SFARI iPS cell lines, and they brainstormed ways in which iPS cell models can be used to aid our understanding of autism spectrum disorder (ASD) and other neurodevelopmental conditions. (sfari.org)
  • An odontogenic medium modified by the addition of recombinant human bone morphogenetic protein 2 was used in the experimental groups to induce differentiation. (scielo.br)
  • culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
  • Borlongan, C.V., Glover, L.E., Tajiri, N., Kaneko, Y. and Freeman, T.B. (2011) The Great Migration of Bone Marrow-Derived Stem Cells towards the Ischemic Brain: Therapeutic Implications for Stroke and Other Neurological Disorders. (scirp.org)
  • These include bone marrow-derived cells, peripheral blood derived stem cells and skeletal myoblasts (3). (escardio.org)
  • In experimental and clinical studies unselected mononuclear bone marrow cells as well as specific subpopulations have been used for transplantation. (escardio.org)
  • My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
  • When the surrounding environment was very stiff, the stem cells grew into bone. (futurity.org)
  • Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)
  • Leukocytes are produced in stem cells in bone marrow. (lu.se)
  • Such cells from different sources can proliferate and differentiate into different lineages (e.g. osteogenic, chondrogenic and adipogenic) after suitable stimulation. (scirp.org)
  • hsa-miR-221 and hsa-miR-124 promoted and hsa-miR-140-5p inhibited neuronal differentiation, hsa-miR-26a-5p promoted and hsa-miR-424 suppressed angiogenic differentiation, and hsa-miR-135 and hsa-miR-143 inhibited differentiation within myogenic lineages. (biomedcentral.com)
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
  • These processes generally involve growth factors (GFs), substances that control cell function through the activation of specific signalling pathways and that are capable of stimulating cellular growth, proliferation, and cellular differentiation. (interface-online.org.uk)
  • Kim and Park, 2017 ) and make HMSCs attractive cells for regenerative therapies. (frontiersin.org)
  • This is an important, unmet need in the field and development of such a substrate would aid in moving stem cell therapies to the clinic. (ca.gov)
  • Tissue bioengineering and stem/progenitor cell treatments have both been individually pursued for stroke neural repair therapies, with some benefit in tissue recovery. (researchgate.net)
  • Emerging directions in stroke neural repair approaches combine these two therapies to use biopolymers with stem/progenitor transplants to promote greater cell survival in the transplant and directed delivery of bioactive molecules to the transplanted cells and the adjacent injured tissue. (researchgate.net)
  • Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
  • This explains why researchers are now focusing attention on developing stem cell therapies using postnatal stem cells donated by the patients themselves or their close relatives. (bvsalud.org)
  • The fine regulation of the brain-derived neurotrophic factor (BDNF) by sortilin is required for both neuronal and tumor cell survival. (wikipedia.org)
  • Furthermore, it appears that sortilin participates in the progression of breast cancer and contributes to tumor cell adhesion and invasion. (wikipedia.org)
  • Cancer stem cells are thought to be responsible for tumor initiation, dissemination and treatment failure. (jcancer.org)
  • The research group "Stem Cells of Skeletal Muscle" lead by Dr. Julia von Maltzahn at the Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) in Jena, Germany, investigated the extracellular ligand Wnt7a and was able to show its positive influence on muscle mass and muscle stem cells in cancer cachexia independently of the tumor cell type causing cachexia. (leibniz-gemeinschaft.de)
  • In addition to basic science investigations in such areas as tumor cell invasion, oncogenes, immunotherapy and genetic inheritance, our scientists and physicians participate in national cancer trials. (upstate.edu)
  • Using this vesicular delivery system, cancer cells can establish a new metastatic niche within distant sites, away from the primary tumor, thus favoring cancer progression. (springer.com)
  • Simple and effective detection methods for circulating tumor cells are essential for early detection and progression monitoring of tumors. (bvsalud.org)
  • The use of DNA aptamer and bioluminescence is expected to be a key tool for the simple, effective, and sensitive detection of tumor cells. (bvsalud.org)
  • Herein, we designed multifunctional protein nanoparticles for the detection of tumor cells using DNA aptamer and bioluminescence. (bvsalud.org)
  • Mucin-1 (MUC1)-overexpressing human breast tumor MCF7 cells and MUC1-recognizing aptamer (MUC1 aptamer) were selected as models. (bvsalud.org)
  • Basal cell carcinoma (BCC) is a nonmelanocytic skin cancer (ie, an epithelial tumor) that arises from basal cells (ie, small, round cells found in the lower layer of the epidermis). (medscape.com)
  • Interleukin (IL)-9-producing subset called Th9 cell, Th22 cells which primarily secrete IL-22, IL-13 and tumor necrosis factor- and Th25 cells via producing IL-25 are believed to be important for initiating allergic reactions and developing airway inflammation. (cdc.gov)
  • The ability to understand and regulate human vasculature development and differentiation has the potential to benefit patients suffering from a variety of ailments, including cardiovascular disease, peripheral. (biomedcentral.com)
  • Zong C, Bronckaers A, Vande Velde G, Willems G, Cadenas de Llano-Pérula M. (2022) In Vivo Micro-Computerized Tomography Tracking of Human Periodontal Ligament Stem Cells Labeled with Gold Nanocomplexes. (uhasselt.be)
  • editors Culture of human stem cells. (scielo.br)
  • We proposed to develop novel, well-defined, synthetic extracellular matrices that support survival and proliferation of human embryonic stem cells. (ca.gov)
  • Finally, we identified a cyclic RGD peptide that supports growth of human embryonic stem cells. (ca.gov)
  • The molecular study of fat cell development in the human body is essential for our understanding of obesity and related diseases. (mdpi.com)
  • Gimble, J.M., Bunnell, B.A. and Guilak, F. (2012) Human Adipose-Derived Cells: An Update on the Transition to Clinical Translation. (scirp.org)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • IPS cells derived from individuals with neurodevelopmental conditions provide a unique tool to examine early developmental changes in molecular and cellular mechanisms within the human genetic context, thus complementing studies that use human postmortem brain tissue and animal models. (sfari.org)
  • IPS cells are the only model that allows longitudinal and mechanistic analyses within relevant human genetic backgrounds that can be related back to current phenotypes in living individuals. (sfari.org)
  • Highlighting the importance of this iPS cell collection, Vaccarino started off the first session saying that "iPS cells are the only model that allows longitudinal and mechanistic analyses within relevant human genetic backgrounds that can be related back to current phenotypes in living individuals. (sfari.org)
  • Human embryonic stem cells also exhibit the Warburg effect ( 15 ). (spandidos-publications.com)
  • The aim of the present study was to evaluate whether Alix is involved in the packaging of miRNAs within EVs released by human liver stem‑like cells (HLSCs). (spandidos-publications.com)
  • The research group was able to show in human skeletal muscle cell lines and in a mouse model that Wnt7a treatment counteracted muscle wasting and improved muscle stem cell differentiation - two major symptoms of cachexia in cancer patients. (leibniz-gemeinschaft.de)
  • Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
  • The MUC1 aptamer-conjugated protein nanoparticles exhibited a 13.7-fold higher bioluminescence signal to MCF-7 cells than to human embryonic kidney 293 (HEK293) cells, which express low levels of MUC1. (bvsalud.org)
  • The possible donor-host rejection of human ES cells is another concern 3 . (bvsalud.org)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • My observations suggest that fibronectin fibrils coating a polymer with low mobility may be most suited for osteogenic differentiation of hMSCs by simultaneously exposing cell-binding sites to a higher degree. (gla.ac.uk)
  • Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
  • Under these conditions, iPS cells begin to differentiate into a hepatocyte lineage. (spandidos-publications.com)
  • A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)
  • Lineage specification is an essential process in stem cell fate, tissue homeostasis and development. (exosome-rna.com)
  • This series covers biophysical regulation of stem cell fate and function in the context of tissue development. (biomedcentral.com)
  • The role and effect of 5hmC on 5mC turnover and hence DNA demethylation, on gene expression per se and stem cell fate and differentiation is a topic of vigorous interest. (stanford.edu)
  • Differentiation of stem cells is known be triggered by various growth factors and regulatory molecules. (justia.com)
  • Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)
  • This molecular function enables sortilin to participate in various biological processes, including the transport of GLUT4 to the plasma membrane of fat and skeletal muscle cells in response to insulin. (wikipedia.org)
  • Medgar Evers College RISE student Zimuzoh K. Orakwue is presenting her research poster on "testosterone effect on molecular signaling in the penis of sickle cell mice" at ABRCMS conference. (cuny.edu)
  • Advances in the fields of stem cell biology, biomaterials, and tissue engineering over the last decades have brought the possibility of constructing tissue substitutes with a broad range of applications in reg. (biomedcentral.com)
  • From the basic biology perspective, these findings have revealed that cell fates are inherently plastic and are dynamically regulated. (stanford.edu)
  • I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
  • I cover mainly cell biology and physiology. (nottingham.ac.uk)
  • These data suggest that the interaction of Thy-1 with beta integrins mediates mEV uptake by lung fibroblasts, which blocks myofibroblastic differentiation, and that mEVs are enriched for miRs that target profibrotic genes up-regulated in IPF fibroblasts. (nih.gov)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
  • To facilitate this type of research in the autism field, SFARI has supported the generation of numerous iPS cell lines from individuals with genetic variants in high-confidence autism risk genes, who enrolled in the Simons Simplex Collection (SSC) and Simons Searchlight studies. (sfari.org)
  • An article collection in Stem Cell Research & Therapy . (biomedcentral.com)
  • In addition, the extracellular ligand could be used as a supportive treatment during therapy/surgery. (leibniz-gemeinschaft.de)
  • T lymphocytes for adoptive cell therapy against hematological cancer. (upstate.edu)
  • Cell therapy is a potential new treatment for post MI patients, but the main challenges are : the necessity to preserve immune competency and to gain adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
  • As such, stem cell therapy cannot be regarded as a valid therapeutic option for patients with cardiovascular disease in the present era of evidenced-based medicine, yet it holds great hope for the future. (escardio.org)
  • Preclinical and some small-scaled clinical trials have suggested feasibility and safety of cardiac stem cell therapy. (escardio.org)
  • Cell therapy is currently emerging as a potential new treatment for post MI patients with the assumption that recolonization of the areas of scarred myocardium with exogenously supplied surrogates or precursors of cardiomyocytes can restore function and ultimately affect clinical outcomes. (escardio.org)
  • Repair of scar tissue constitutes a challenge for cardiac stem cell therapy due to lack of adequate nutrition and homing signals necessary for stem cells' engraftment and survival. (escardio.org)
  • Although MSC-derived EVs (mEVs) are beneficial for acute lung injury and pulmonary fibrosis, mechanisms of mEV uptake by lung fibroblasts and their effects on myofibroblastic differentiation have not been established. (nih.gov)
  • Nevertheless, a lack of knowledge relating to the mechanisms regulating their differentiation has limited their clinical application. (biomedcentral.com)
  • iPS cells can be grown and differentiated into many different brain cell types, as well as 3D brain organoids 1,2 . (sfari.org)
  • Etoc noted the emphasis that RUMI Scientific has placed on micropatterning technologies 3,4 , which can standardize stem cell differentiation and the geometry of organoids by providing a lattice often comprised of extracellular matrix components. (sfari.org)
  • miRNAs involved in odontogenic/osteogenic differentiation were then included in a bioinformatic analysis. (biomedcentral.com)
  • I work on various biochemical and biophysical approaches to investigate and control cell-cell, cell-scaffold interactions. (nottingham.ac.uk)
  • The researchers used a specifically tailored hydrogel to "scaffold" and control an implant containing stem cells to form a functional brown-fat-like tissue. (futurity.org)
  • The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)
  • The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. (justia.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • Cancer cells have proven efficient in educating fibroblasts, within their microenvironment, to secrete EVs as communicative vessels for mediating phenotypic changes in recipient cells. (springer.com)
  • Overview of the Immune System The immune system distinguishes self from nonself and eliminates potentially harmful nonself molecules and cells from the body. (msdmanuals.com)
  • Although some antigens (Ags) can stimulate the immune response directly, T cell-dependent acquired immune responses typically require antigen-presenting cells (APCs) to present antigen-derived peptides within major histocompatibility complex (MHC) molecules. (msdmanuals.com)
  • Intracellular antigens (eg, viruses) can be processed and presented to CD8 cytotoxic T cells by any nucleated cell because all nucleated cells express class I MHC molecules. (msdmanuals.com)
  • Extracellular antigens (eg, from many bacteria) that are phagocytosed or endocytosed can be processed into peptides and complexed with surface class II MHC molecules on professional APCs, which specialize in presenting antigens to CD4 helper T (Th) cells. (msdmanuals.com)
  • Follicular dendritic cells are a distinct lineage (a different cell type than conventional dendritic cells), do not express class II MHC molecules, and therefore do not present antigen to Th cells. (msdmanuals.com)
  • A significant role for sortilin has recently also been reported in the field of oncology, as it has been detected in several cancer cell lines. (wikipedia.org)
  • Therefore, we hypothesized that CRC cancer stem cell markers (CRCSC) will identify a group of patients at high risk for progression. (jcancer.org)
  • Cancer cells continue to produce lactate via glycolysis when oxygen is abundant (the Warburg effect) ( 14 ). (spandidos-publications.com)
  • Although, several approaches to target cancer cell secretion of EVs are detailed in the literature, there is still no defined way to currently apply them in clinical settings. (springer.com)
  • Cancer Cell 30(6):836-848. (springer.com)
  • DNA aptamer-displaying nanoparticles encapsulating the anti-cancer drug paclitaxel were able to bind to cells overexpressing MUC1 and induce cell death. (bvsalud.org)
  • Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)