Extracellular osteopontin. Medical search. Frequent questions

Osteopontin (OPN), also known as bone /sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric), is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). (wikipedia.org)
Allogro® causes a significant induction of osteoblast transcriptional factor like osterix (RUNX2) and of the bone related genes osteopontin (SPP1), osteocalcin (BGLAP) and alkaline phosphatase (ALPL). (ispub.com)
To date, SPP1 (osteopontin) 8 and LTBP4 (latent TGF-β binding protein 4) 9 have been identified as genetic modifiers in both human DMD and murine models of disease. (nature.com)
Secreted phosphoprotein 1 (SPP1, osteopontin) binds to integrin alphavbeta6 on porcine trophectoderm cells and integrin alphavbeta3 on uterine luminal epithelial cells, and promotes trophectoderm cell adhesion and migration. (nih.gov)
Osteopontin (SPP1) upregulation increased from 30-fold at Day 30 to 45-fold at Day 14. (thejns.org)
Osteopontin (OPN) also called secreted phosphoprotein 1 (SPP1) is really 1Mps1-IN-1 a soluble protein that's within most body liquids. (researchatlanta.org)
Evaluation of SPP1/osteopontin expression as predictor of recurrence in tamoxifen treated breast cancer. (cdc.gov)

Bim deficient astroglial cells also exhibited altered proliferation, migration, adhesion to various extracellular matrix proteins and increased expression of inflammatory mediators. (nih.gov)
GO and KEGG analysis revealed that differentially expressed proteins participated in the regulation of several pathways including oxidative phosphorylation, ribosome, extracellular matrix interaction, and PI3K-Akt pathway. (peerj.com)
This subunit may associate with a beta 1 or beta 4 subunit to form an integrin that interacts with extracellular matrix proteins including members of the laminin family. (cancerindex.org)
Investigators have demonstrated other substrates for factor XIIIa, including proteins such as osteopontin, factor V, thrombospondin, vinculin, and endothelial cell receptors αvβ3 integrin and VEGFR-2. (medscape.com)
The impaired cross-linking of extracellular matrix proteins at sites of wound healing or placental implantation can lead to abnormalities in these processes, resulting in abnormal or delayed healing and spontaneous abortion. (medscape.com)
In vitro hybridisation has shown that osteopontin is produced by a subset of lesion macrophages, while bone morphogenetic proteins 2 and 4 are expressed by myofibroblasts, in areas adjacent to lymphocyte infiltration. (bmj.com)
The DisProt curation team is pleased to report a new release featuring the second thematic DisProt dataset: "extracellular matrix proteins", describing the role of IDPs in this network within the tissues. (github.io)
The extracellular matrix (ECM) provides structural and biochemical support to the surrounding tissues, as a three-dimensional network composed of proteins and polysaccharides that includes collagen, elastin, laminin, fibronectin and a large number of glycosaminoglycans. (github.io)
Here, we annotated and reviewed 46 IDPs playing a role in the extracellular matrix and covered examples of mostly human proteins (90%), but also mouse, rat and pig proteins. (github.io)
The Extracellular Matrix is a dynamic entity, showing constant degradation and deposition while providing the framework for the cardiomyocytes and interstitial proteins to lie on. (pitt.edu)
While other constitutively expressed proteins in the extracellular matrix, such as osteopontin and osteocalcin, are expressed in osteoblast cells, DMP1 is expressed in osteocytes, making it a candidate biomarker for osteocyte activity. (takarabio.com)
Postn can be a more lately added person in the matricellular proteins family a course of nonstructural ECM protein that also contains Tnc ��ig-h3 osteopontin secreted proteins acidic and abundant with cysteine (SPARC) thrombospondin-1 in addition to others [21]. (academicediting.org)
Tumor cells home in to bone via the same protein interactions that hematopoietic stem cells use, relying on integrins, chemokines, bone morphogenetic proteins, and osteopontin, among others, to settle in bone. (basicmedicalkey.com)
Using our newly developed micro-volume chemotaxis assay, we developed an improved quantitative method to measure in vitro chemotaxis of smooth muscle or endothelial cells toward different extracellular matrix proteins. (johnshopkins.edu)
In addition, the ANKH protein transports a molecule called pyrophosphate out of cells to the intricate network of proteins that forms in the spaces between cells (extracellular matrix). (medlineplus.gov)
Eight of the 24 human integrins, which resemble the evolutionarily most ancient metazoan integrins, can recognize RGD and RGD-like sequence patterns in their ligands: components of the extracellular matrix (ECM), cell surface proteins of cells or other extracellular signalling proteins. (eu.org)

Sp7/Osterix regulates the expression of a number of important osteoblast genes such as osteocalcin, osteonectin, osteopontin, bone sialoprotein and type I collagen. (biomedcentral.com)
Concomitant expression of osterix, bone sialoprotein (BSP), osteopontin, and osteocalcin was modulated by addition or removal of doxycycline under osteogenic guidance. (elsevierpure.com)

TGF-beta1 is involved in fibrotic remodeling including fibroblast differentiation and enhanced deposition of collagen in the extracellular matrix. (cdc.gov)
Vascular homeostasis is maintained by the extracellular matrix (ECM) and related cell surface receptors, such as integrins, characterised by collagen deposition, collagen crosslinking, and elastin degradation within the vascular ECM. (biomedcentral.com)
Some notable examples include collagen (DP02163) , MMP (DP03257) , osteopontin (DP00214) and LOX (DP02075) . (github.io)
Ricard-Blum S, Ruggiero F. The collagen superfamily: from the extracellular matrix to the cell membrane. (pitt.edu)
Postn performing via its EMI site binding collagen type I and fibronectin and its own adjacent FAS1 site binding BMS-708163 Tnc was further proven to work as a molecular bridge to aid the incorporation of Tnc in to the ECM therefore acting to create a particular hexabrachion framework that may boost and stabilize bifurcations of collagen type I and fibronectin fibrils built-into an extracellular meshwork structures [32]. (academicediting.org)
Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). (arigobio.cn)

Our previous studies identified that osteopontin (OPN), a pro-fibrotic cytokine, is abundant in the prostate of men with LUTS, and its secretion is stimulated by inflammatory cytokines potentially to drive fibrosis. (nih.gov)
Exposure of the lung to carbonaceous nanoparticle s: single walled carbon nanotube s (SWCNT) leads to the development of pulmonary fibrosis (PF) and induction of a network of pro-fibrotic/pro-inflammatory cytokines including TGF-beta1 and osteopontin (OPN). (cdc.gov)
The lists of up- and downregulated genes were cross-matched with lists of genes known to be associated with cytokines or the extracellular matrix. (thejns.org)
Hematopoiesis occurs in the spaces between sinuses and is controlled by a complex array of stimulatory and inhibitory cytokines, cell-cell contacts, and extracellular matrix components. (mhmedical.com)

Phosphorylated osteopontin (Phospho-OPN) is known to increase macrophage and osteoclast activation, this process is controlled by extracellular tartrate-resistant acid phosphatase (TRAcP), also a biomarker for RA. (researcher-app.com)

It was shown that osteopontin (OPN), a glycophosphoprotein, plays divergent roles in cancer progression. (unideb.hu)

Osteopontin is a SIBLING (glycoprotein) that was first identified in 1986 in osteoblasts. (wikipedia.org)
Osteopontin is a secreted matricellular glycoprotein that signals partially through integrin receptors and its downregulation is associated with increased strength, reduced fibrosis, and milder muscle pathology 10 , 11 . (nature.com)
Periostin is really a modular glycoprotein frequently observed to be always a major constituent from the extracellular milieu of mass-forming intrahepatic cholangiocarcinoma along with other desmoplastic malignant tumors. (academicediting.org)

Cell signaling by osteopontin may occur through a cell adhesion sequence that recognizes INTEGRIN ALPHA-V BETA-3 . (nih.gov)
CD44 is an adhesion molecule for extracellular matrix components such as hyaluronic acid and osteopontin [ 7 ], and plays an important role not only in wound healing and cell migration, but also in tumor invasion and metastasis. (biomedcentral.com)
In addition to multiple intra- and extracellular functions, it facilitates migration of tumour cells, has crucial role in cell adhesion and is associated with increased metastasis formation. (unideb.hu)
1,4 Furthermore, adhesion molecules such as osteopontin and bone sialoproteins which regulate mineralization, are secreted by ERM in the root regions of mature and developing teeth. (scielo.org.za)

Osteopontin (OPN) is an extracellular matrix protein that is cleaved by thrombin, generating the N-terminal half of OPN, which is prominently involved in integrin signal transduction. (mdpi.com)
Osteopontin is a ligand for the alpha4beta1 integrin. (tamu.edu)

Addison WN, Azari F, Sorensen ES, Kaartinen MT, McKee MD. Pyrophosphate inhibits mineralization of osteoblast cultures by binding to mineral, up-regulating osteopontin, and inhibiting alkaline phosphatase activity. (medlineplus.gov)

OPN is a highly negatively charged, heavily phosphorylated extracellular matrix protein that lacks an extensive secondary structure as an intrinsically disordered protein. (wikipedia.org)
A negatively-charged extracellular matrix protein that plays a role in the regulation of BONE metabolism and a variety of other biological functions. (nih.gov)

1991 . The murine gene encoding secreted phosphoprotein 1 (osteopontin): promoter structure, activity, and induction in vivo by estrogen and progesterone. (nih.gov)

Oxidative nanopatterning of titanium surfaces promotes production and extracellular accumulation of osteopontin. (bvsalud.org)
CNT-induced fibrosis involves a multitude of cell types and is characterized by the early onset of inflammation, oxidative stress and accumulation of extracellular matrix. (cdc.gov)

Moreover, Millán and his colleagues identified yet another player in the regulation of bone calcification: a protein called osteopontin. (nih.gov)
Enhanced extracellular matrix calcification was observed when forced Amelx expression commenced at the early stage but not at the intermediate or late stages of osteogenesis. (elsevierpure.com)

1. miRNA and long non-coding RNA transcriptional expression in hepatocellular carcinoma cell line-secreted extracellular vesicles. (nih.gov)
2. Tumor-derived extracellular vesicles containing long noncoding RNA PART1 exert oncogenic effect in hepatocellular carcinoma by polarizing macrophages into M2. (nih.gov)
3. Tumor cells derived-extracellular vesicles transfer miR-3129 to promote hepatocellular carcinoma metastasis by targeting TXNIP. (nih.gov)
4. Transcriptional level evaluation of osteopontin/miRNA-181a axis in hepatocellular carcinoma cell line-secreted extracellular vesicles. (nih.gov)
7. Extracellular Vesicles-Encapsulated MicroRNA-125b Produced in Genetically Modified Mesenchymal Stromal Cells Inhibits Hepatocellular Carcinoma Cell Proliferation. (nih.gov)
10. Bone mesenchymal stem cells derived extracellular vesicles promote TRAIL-related apoptosis of hepatocellular carcinoma cells via the delivery of microRNA-20a-3p. (nih.gov)

In mouse studies, the researchers have found that osteopontin levels are highly correlated with pyrophosphate levels, yet they don't know yet if the two are acting in concert or if one controls the other. (nih.gov)
Researchers believe that these mutations lead to elevated pyrophosphate levels in the extracellular matrix. (medlineplus.gov)

Stem cell differentiation has been extensively studied with various microenvironment mimicking structures to modify cellular dynamics associated with the cell-extracellular matrix (ECM) interactions and cell-cell communications. (yyu.edu.tr)
The role of extracellular OPN in MSC differentiation was confirmed by supplementation and neutralization of OPN further. (researchatlanta.org)

Dentin matrix protein 1 (DMP1) is an acidic extracellular matrix phosphoprotein that can bind calcium. (takarabio.com)

Lindsey ML, Hall ME, Harmancey R, Ma Y. Adapting extracellular matrix proteomics for clinical studies on cardiac remodeling post-myocardial infarction. (pitt.edu)

Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors. (arigobio.cn)

Associations between the Genetic Polymorphisms of Osteopontin Promoter and Susceptibility to Cancer in Chinese Population: A Meta-Analysis. (cdc.gov)

Osteopontin (OPN) expression is modulated by Schistosoma mansoni egg antigen. (wikipedia.org)

However, osteopontin is synthesized in various tissues and cell types, thereby affecting cellular immunity by regulation of cell infiltration, restoration of tissues, angiogenesis, inhibition of cell death, and reconstitution of ECM. (medscape.com)
Bim is a pro-apoptotic protein that modulates not only apoptosis but also various cellular functions such as migration and extracellular matrix protein expression. (nih.gov)
In order to engineer an environment supporting bone formation, combinations of biochemical and biophysical signals need to be presented to the cells in a three-dimensional setting in a way that allows cellular interactions with the surrounding cells and extracellular matrix (Figure 1 , left). (biomedcentral.com)
In conclusion, exposure to MWCNT or asbestos induced gene expression of CD44 and OPN, protein release of OPN, and modifications of the interaction between CD44 and OPN at cellular and extracellular levels. (cdc.gov)
Additionally, a selective αvβ3 small molecule peptiddomimetic, monoclonal antibody LM609, or an anti-β3 (αvβ3/αIIβ3) antibody, c7E3 demonstrated maximal inhibition of cellular migration toward vitronectin or osteopontin. (johnshopkins.edu)

Wound healing is promoted by factor XIIIa via cross-linking of the provisional matrix, a process that influences the extracellular matrix-leukocyte interaction. (medscape.com)

Synthesis of osteopontin is stimulated by calcitriol (1,25-dihydroxy-vitamin D3). (wikipedia.org)
However, in experimental mice models, mechanical stress (i.e. pressure and volume loading) as well as hypoxia strongly induced osteopontin synthesis in the heart. (medscape.com)

In mature osteoblasts, the DMP1 protein is phosphorylated and localized to the extracellular matrix, where it plays a role in forming mineralized matrix. (takarabio.com)
In vitro, GnRH-induced expression of this gene is coupled with release of DMP1 in extracellular medium through the regulated secretory pathway. (nih.gov)

We showed that astroglial cells lacking Bim expression demonstrate increased VEGF expression and altered matricellular protein production including increased expression of thrombospondin-2 (TSP2), osteopontin and SPARC. (nih.gov)

It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). (arigobio.cn)

Intracellular OPN is generated using an alternative translation start site on the same mRNA species used to generate the extracellular isoform. (wikipedia.org)
Body composition: Measures included fat-free mass, fat mass, phase angle, extracellular mass-to-body cell mass ratio (ECM/BCM), total body water, and intracellular water. (naturalmedicinejournal.com)

Osteopontin is an extracellular structural protein and therefore an organic component of bone. (wikipedia.org)
Extracellular matrix, mechanotransduction and structural hierarchies in heart tissue engineering. (pitt.edu)

In healthy cardiac tissue, osteopontin has been found to be expressed only at low levels. (medscape.com)
and tissue nonspecific alkaline phosphatase (TNAP), which breaks down pyrophosphate in the extracellular environment, keeping its levels in check. (nih.gov)

This extracellular pyrophosphate helps regulate bone formation by preventing mineralization, the process by which minerals such as calcium and phosphorus are deposited in tissues. (medlineplus.gov)
A shortage of extracellular pyrophosphate can increase bone mineralization, which may also contribute to the bone abnormalities. (medlineplus.gov)

2012 . Osteopontin is an endogenous modulator of the constitutively activated phenotype of pulmonary adventitial fibroblasts in hypoxic pulmonary hypertension. (nih.gov)

Regulation of the osteopontin gene expression is incompletely understood. (wikipedia.org)

2004 . Altered bleomycin-induced lung fibrosis in osteopontin-deficient mice. (nih.gov)

Aims To evaluate the diagnostic and prognostic value of osteopontin in patients with acute dyspnoea and/or peripheral oedema suspected of having acute congestive heart failure (aCHF). (medscape.com)
Additionally, osteopontin vs. NT-proBNP for prognostic outcomes (i.e. all-cause mortality, aCHF-related rehospitalization, and both in combination) was tested. (medscape.com)
The diagnostic and prognostic capacity of osteopontin was tested by C-statistics, reclassification indices, and multivariable Cox prediction models. (medscape.com)
Compared with NT-proBNP, osteopontin was of superior prognostic value, specifically in aCHF patients and for the prognostic outcome of aCHF-related rehospitalization. (medscape.com)
Osteopontin identifies aCHF patients with high 1- and 5-year mortality and rehospitalization risk, and adds prognostic value to NT-proBNP. (medscape.com)
Therefore, the present study aimed to investigate the diagnostic and prognostic value of measurements of osteopontin plasma levels in comparison with NT-proBNP within a prospective controlled trial enrolling patients initially presenting with symptoms of acute dyspnoea or peripheral oedema suspected of aCHF in the emergency department. (medscape.com)

Using this method, we have determined that either vitronectin at 0.4 μg/well or osteopontin at 0.4 μg/well are selective αvβ3 chemoattractants for endothelial or smooth muscle cells (0.5 x 10 5 cells/well). (johnshopkins.edu)

Mechanism of multiwalled carbon nanotube- and asbestos -induced osteopontin/CD44 in Raw 264 macrophages. (cdc.gov)
Recently we demonstrated that this response is associated with activation of osteopontin (OPN) in lung macrophages, which is similar to the effects of asbestos exposure. (cdc.gov)

[ 1 ] Osteopontin represents a central member of the matricellular protein family, being initially found in bone matrix. (medscape.com)

Role of extracellular matrix in the pathogenesis of cerebral vasospasm. (biomedcentral.com)

The imbalance of extracellular matrix homeostasis is an important cause of vascular remodelling. (biomedcentral.com)
YAP pathway regulates the proliferation of vascular cells through the extracellular matrix. (biomedcentral.com)
Se asocia con la calcificación vascular y con una gran mortalidad cuando sus niveles en sangre son elevados. (revistanefrologia.com)

Extracellular OPN features through its connections with multiple ubiquitously-expressed cell surface area receptors including several integrins (αvβ1 αvβ3 αvβ5 α4β1 α8β1 and α9β1) and Compact disc44 [16]. (researchatlanta.org)

Linked deficiencies in extracellular inorganic pyrophosphate (PPi) and osteopontin expression mediate pathologic ossification in PC-1 null mice. (nih.gov)

Osteopontin-c has been suggested to facilitate the anchorage-independent phenotype of some human breast cancer cells due to its inability to associate with the extracellular matrix. (wikipedia.org)
Extracellular polyphosphate signals through Ras and Akt to prime Dictyostelium discoideum cells for development. (tamu.edu)

We developed an on-slide decellularization approach to generate acellular extracellular matrix (ECM) myoscaffolds that can be repopulated with various cell types to interrogate cell-ECM interactions. (nature.com)

Serum biomarkers of extracellular degradation products, selective metalloproteinase inhibitors and a personalized treatment approach with a revisal of the current classification of heart failure are topics requiring further exploration. (pitt.edu)

RGD composite binding site is formed by both alpha and beta subunits of integrins and is located on the extracellular side of plasma membrane. (eu.org)

Eta-1 (osteopontin): an early component of type-1 (cell-mediated) immunity. (bdbiosciences.com)

Conclusion Osteopontin improves aCHF diagnosis when combined with NT-proBNP. (medscape.com)

Anatomy1

Diseases1

Chemicals and Drugs7

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes4

Disciplines and Occupations2

Information Science2

Spp17

Proteins16

Osteocalcin2

Collagen6

Cytokines4

Tartrate-Resistant A1

Shown that osteopontin1

Glycoprotein3

Adhesion4

Integrin2

Alkaline phosphatase activity1

Negatively charged2

Encoding secreted1

Oxidative2

Calcification2

Vesicles6

Pyrophosphate levels2

Differentiation2

Acidic1

Proteomics1

Proteinases1

Promoter1

Antigen1

Cellular5

Interaction1

Synthesis2

DMP12

SPARC1

Receptor1

Intracellular2

Structural2

Tissue2

Mineralization2

Constitutively1

Gene expression1

Fibrosis1

Prognostic6

Endothelial1

Macrophages2

Matricellular1

Role1

Vascular3

Receptors1

Expression1

Cells2

Interactions1

Selective1

Subunits1

Cell1

Conclusion1