Extracellular dopamine (DA) and its metabolite DOPAC were also measured in the nucleus accumbens after systemic drug administration, using in vivo microdialysis in freely moving rats. (erowid.org)
In vivo microdialysis was used to evaluate the acute actions of ibogaine and 12-hydroxyibogamine on the levels of DA and 5-HT. (nih.gov)
Using in vivo microdialysis, release of ACh was measured in the hippocampus while rats learned and were tested on an amygdala-dependent CPP task and a hippocampus-dependent spontaneous alternation task. (jneurosci.org)
Microdialysis further reveals that these modes and action potential-independent release provide significant amounts of extracellular dopamine in vivo. (elifesciences.org)
We examined the effect of three months of rosiglitazone treatment (4 mg b.i.d.) on whole-body insulin sensitivity and in vivo peripheral adipocyte insulin sensitivity as assessed by glycerol release in microdialysis from subcutaneous fat during a two-step (20 and 120 mU · m −2 · min −1 ) hyperinsulinemic-euglycemic clamp in nine type 2 diabetic subjects. (diabetesjournals.org)
Fifteen hours after the final pretreatment, basal extracellular concentrations and "in vivo extraction fraction" values for DA and 5-HT were determined by no-net-flux in vivo microdialysis. (aspetjournals.org)
First, to date, evidence on monoaminergic differences between high and low P rats is based largely on ex vivo, whole-tissue measures of DA and 5-HT rather than extracellular measures of transmitter release. (aspetjournals.org)
We use in vivo microdialysis, EEG recordings including spectral analysis, behavioral analyses. (esrs.eu)
A neurochemical heterogeneity of the rat striatum as measured by in vivo electrochemistry and microdialysis. (omeka.net)
In addition, using in vivo microdialysis, we found that basal extracellular striatal dopamine levels were reduced by 70% in R6/1 mice compared to their wild-type littermates. (lu.se)
Concentrations10
Basal extracellular concentrations of NE, DA, and 5-HT were determined over a 1-h period using HPLC. (harvard.edu)
In rats exposed to PPM there was a decrease in extracellular concentrations of NE and DA in the right vmPFC and an increase in the extracellular concentration of 5-HT in the left vmPFC compared to controls (prenatally malnourished: N = 10, well-nourished: N = 20). (harvard.edu)
To test this hypothesis, basal extracellular concentrations of dopamine (DA) and serotonin (5-HT) in the nucleus accumbens as well as the effects of repeated ethanol pretreatment on the basal release of these transmitters were examined in alcohol-preferring (P), alcohol-nonpreferring (NP), and genetically heterogeneous Wistar rats. (aspetjournals.org)
In addition, ethanol pretreatment increased extracellular DA concentrations in Wistar and P rats, but not in NP rats. (aspetjournals.org)
The results showed a significant longitudinal decrease in extracellular glutamate concentrations in the motor cortex and hippocampus in MCAO+AP39 rats compared to MCAO rats. (bvsalud.org)
Upregulation of the GLT-1 transporter responsible for glutamate reuptake from the synaptic cleft, and downregulation of VGLUT1, which regulates glutamate transport to synaptic vesicles, indicate that these are important mechanisms by which AP39 reduces extracellular glutamate concentrations and, consequently, excitotoxicity after ischemia. (bvsalud.org)
This compound increases extracellular glutamate concentrations, lipid peroxidation, the number of microglia cells and induces process of apoptosis. (bvsalud.org)
Severe encephalopathy resulted in a significant loss of [3H]-nisoxetine sites in frontal cortex and a concomitant increase in extracellular brain concentrations of NA in rats with acute liver failure. (hepato-neuro.ca)
Only a few studies have addressed the effect of sleep deprivation on extracellular adenosine concentrations in other brain regions. (nin.nl)
The 64 h microdialysis experiment determined the extracellular adenosine and adenosine monophosphate (AMP) concentrations in the medial prefrontal cortex of rats before, during and after 12 h of sleep deprivation by forced locomotion. (nin.nl)
Basal extracellular1
Ethanol pretreatment decreased basal extracellular 5-HT levels in P rats whereas increasing 5-HT efflux was seen in the Wistar and NP lines. (aspetjournals.org)
Probes7
Before making microsurgical lesions, specific concentric-type microdialysis probes were implanted over a 60-minute period in the DREZ and directed to the DH apex (10 implantations). (thejns.org)
At 120 days of age, 2 mm microdialysis probes were placed into left and right vmPFC. (harvard.edu)
The use of probes with large pore size membranes required for the microdialysis of large molecules creates some methodological challenges such as protein aggregation, protein adhesion, ultrafiltration and low fluid recovery/extraction efficiency. (harvardapparatus.com)
CMA offers a set of microdialysis probes for the recovery of large molecules (such as proteins, cytokines, growth factors, antibody and neuropeptides…) on awake rodents. (harvardapparatus.com)
These models were constructed based on linear and concentric microdialysis probes. (usm.my)
Modelling domain of these mathematical models would focus on the microdialysis probes, the membrane attached to the probes, and the probe surrounding area (PSA). (usm.my)
In this case, convection-enhanced diffusion equations are necessary to represent the mass transport phenomena across the membrane attached to microdialysis probes. (usm.my)
Elevates extracellular1
We report here that 12-hydroxyibogamine, a primary metabolite of ibogaine, displays high affinity for the 5-HT transporter and elevates extracellular 5-HT. (nih.gov)
In contrast to the effects seen with MDMA, no difference in extracellular DA or DOPAC was seen after injection of MBDB, fenfluramine, or MMAI, even though MBDB weakly induced a place preference. (erowid.org)
Both doses of CAB caused a decrease in extracellular DOPAC. (erowid.org)
Concentration3
LC-MS was used to determine the extracellular glutamate concentration in microdialysates collected intrasurgically and from freely moving animals 24 h and 3 days after reperfusion (n=6). (bvsalud.org)
Microdialysis is a well-known sampling technique in medical researches, most commonly used to measure the concentration of chemicals in the extracellular space of tissues. (usm.my)
The main objective of this research work is to develop finite element mathematical models that could provide accurate simulations of concentration and fluid flow profiles for microdialysis. (usm.my)
Fluid5
Two adjacent microdialysis catheters that were identical in membrane length, perfusion fluid, and flow rate showed equivalent results. (thejns.org)
A microdialysis technique was used to sample AAs in the extracellular fluid of the DH apex in eight patients in whom surgery in the dorsal root entry zone (DREZ) was performed. (thejns.org)
We recommend the use of the CMA Microdialysis CNS Perfusion fluid with Dextran 500 kDa 3% in order to stabilize the pressure within the probe and limit ultra-filtration. (harvardapparatus.com)
Although there are already several mathematical modelling works on microdialysis, those works would focus only on providing accurate estimations of the recovery, while other features such as fluid flows are neglected. (usm.my)
Cerebral2
Clinical microdialysis enables monitoring of the cerebral extracellular chemistry of neurosurgical patients. (thejns.org)
Assessment of the cerebral laterality of extracellular neurotransmitters in the vmPFC showed that prenatally malnourished animals had a significant shift in laterality from the right to the left hemisphere for NE and DA but not for serotonin. (harvard.edu)
Dopamine levels1
When the rat recovers from the surgery, researchers are able to use the microdialysis device to measure changes in dopamine levels in a living, "freely moving" specimen. (drugscience.org)
Rats1
Microdialysis in freely moving rats is a new technology that allows researchers to implant measuring devices in the rat brain. (drugscience.org)
Subcutaneous1
2. Subcutaneous injection of MKC-242 (0.5 mg kg-1) increased extracellular levels of NA and its metabolite, 3-methoxy-4-hydroxyphenylglycol, in the hypothalamus and hippocampus. (biopsychiatry.com)
Freely1
The present study describes the effect of a highly potent and selective 5-HT1A receptor agonist, 5-(3-[[(2S)-1,4-benzodioxan-2-ylmethyl)]amino]propoxy)-1,3-b enzodioxole HC1 (MKC-242), on NA release in the hypothalamus using microdialysis in the freely moving rat. (biopsychiatry.com)
Nucleus1
Using chemogenetics and microdialysis, we show that, in sign-trackers, stimulation of the neuronal pathway from the prelimbic cortex (PrL) to the paraventricular nucleus of the thalamus (PVT) decreases the incentive value of a reward-cue. (elifesciences.org)
Dialysate2
Samples of extracellular dialysate were collected every 20 min for at least 2 h and analyzed using high-performance liquid chromatography (HPLC) with an electrochemical detector. (scienceblogs.com)
A specially designed bolt was used to conduct two adjacent microdialysis catheters into the frontal cortex of patients with head injury or poor-grade subarachnoid hemorrhage who were receiving ventilation. (thejns.org)
Probe5
The ability of the probe to track the changes of extracellular AAs was demonstrated. (thejns.org)
For instance, microdialysis sampling of glucose under perfused solution flow rate of 1.0 μL min-1 using commercially available liner probe and concentric probe (10 mm membrane length, 30 kDa molecular weight cut-off) yield recovery of 30.98% and 36.67%, respectively. (usm.my)
In addition, although microdialysis is traditionally defined as a diffusion-limited process, it is shown in this work that under combinations of high perfused solution flow rate in the probe, high membrane porosity, and large membrane pore diameter, the influence of convective flux on the recovery of microdialysis sampling would be significant. (usm.my)
Brain1
Monitoring Neurochemistry in Traumatic Brain Injury Patients Using Microdialysis Integrated with Biosensors: A Review. (cam.ac.uk)
Experiment1
In this paper, we describe a microdialysis experiment as well as a meta-analysis of published data. (nin.nl)
The meta-analysis comprised published sleep deprivation animal experiments measuring adenosine by means of microdialysis. (nin.nl)
Significantly1
After absolute quantifications extracellular miRNA-21 was found to be significantly increased in breast cancer. (nih.gov)
Samples1
Our data revealed tissue specific changes of extracellular circulating miRNAs that would be otherwise unraveled using blood samples. (nih.gov)
Amounts1
However, despite being a well-established technique, microdialysis often gives inconsistent amounts of chemicals collected from the sampling site (i.e. recovery). (usm.my)
Technique1
The use of the microdialysis technique for the recovery of large molecules is of increasing interest as it offers the opportunity to identify patterns of protein expression in a variety of tissue spaces as well as to evaluate useful biomarkers of disease. (harvardapparatus.com)
Differences1
it will be important to examine neurochemical differences between high and low P animals at the extracellular level. (aspetjournals.org)
Study2
The present study demonstrates that microdialysis can be performed safely in the human DH during DREZ lesioning. (thejns.org)
In contrast, 11 mg/kg CAB caused no increase and 22 mg/kg CAB caused only a slight increase in extracellular DA. (erowid.org)
Data1
Extracellular circulating microRNAs (miRNAs) have been suggested to be biomarkers for disease monitoring but data are inconsistent, one reason being that blood miRNA is of heterogeneous origin. (nih.gov)
Techniques1
To address this, recent advances have focussed on developing biosensing techniques for integration with microdialysis to achieve continuous online monitoring. (cam.ac.uk)
Recovery1
In order to resolve this issue, it is necessary to understand the mass transport limitations of microdialysis set-up, by scrutinizing how each operational and design parameters of the microdialysis set-up affect the recovery. (usm.my)