Extracellular matrix protein laminin. Medical search. Frequent questions

Antibodies to TIGR/MYOC, fibronectin, laminin, type IV collagen, or thrombospondin were used to determine the extracellular localization of TIGR/MYOC. (nih.gov)
Solid phase binding assays using 125I-recombinant TIGR/MYOC and types I and IV collagens, fibronectin, and laminin were done to examine the association of TIGR/MYOC with these proteins and to identify a specific TIGR/MYOC binding site within fibronectin. (nih.gov)
TIGR/MYOC colocalized with fibronectin, laminin, and type IV collagen, but not thrombospondin in both dexamethasone and dexamethasone/ascorbate-treated HTM cultures and in TM-1 cultures transfected with TIGR/MYOC cDNA. (nih.gov)
In solid phase binding assays, 125I-TIGR/MYOC bound fibronectin but not laminin or type IV collagen. (nih.gov)
The interactions with the Hep II domain of fibronectin could alter cell-matrix interactions in the TM and provides an interesting lead to explore the role(s) of TIGR/MYOC in both steroid-induced and primary open angle glaucoma. (nih.gov)
A nanoscale modular design strategy was employed to synthesize six engineered, recombinant proteins intended to mimic aspects of the extracellular matrix proteins fibronectin, laminin, and elastin as well as the cell-cell adhesive protein neural cell adhesion molecule. (frontiersin.org)
As confirmation that ligand density in these engineered systems impacts neuronal cell behavior, we demonstrate that increasing the density of fibronectin-derived RGD ligands on coated surfaces while maintaining uniform protein surface coverage results in enhanced neurite extension of PC-12 cells. (frontiersin.org)
Desmoplasia is the result of the activation and increased proliferation of myofibroblastslike cells, in particular pancreatic stellate cells (PSCs) and cancer-associated fibroblast (CAFs), and is also accompanied by augmented deposition of different extracellular matrix (ECM) proteins, such as collagens, hyaluronans, laminins and fibronectin. (cnr.it)
Plates are consistently coated with Collagen Type 1, Fibronectin, Laminin, Poly-D-Lysine, or Poly-L-Lysine, and feature a "Best Before" date and lot number. (thomassci.com)
Extracellular matrix proteins Collagens IVI Fibronectin Laminin Tenascin Vitronectin (S-protein) 8. (forextipsnow.com)
MECs synthesize key extracellular matrix (ECM) proteins of the meninges such as various collagens, as well as fibronectin, laminin and tenascin, important for pia basal lamina integrity and thus for neuronal support and function [ 7 ]. (biomedcentral.com)

There are a number of families of extracellular matrix (ECM) proteins including collagens, proteoglycans and laminins (LM). (urosario.edu.co)

They are a major component of the basal lamina (one of the layers of the basement membrane), a protein network foundation for most cells and organs. (pearltrees.com)

Laminins are found in an intricate lattice of proteins and other molecules that forms in the spaces between cells (the extracellular matrix). (medlineplus.gov)
Protein adsorption on a range of hydrophobic and hydrophilic polymer surfaces is typically 0.3-12 mg/m 2 (~500-20,000 molecules/micron 2 ) [ 1322 ]. (nanomedicine.com)
Neural factors are a class of protein molecules with neurotrophic activity that can promote the survival and regeneration of nerve cells. (acrobiosystems.com)
OThe molecules of the extracellular matrix play an important role during cell development and morphogenesis. (bvsalud.org)

Thrombospondin 1, abbreviated as THBS1, is a protein that in humans is encoded by the THBS1 gene. (pearltrees.com)
Several ECM proteins such as laminin, tenascin and thrombospondin contain domains with homology to epidermal growth factor (EGF) and exhibit growth promoting activity. (embl.de)

Antimyosin antibody recognizes laminin, an extracellular matrix alpha-helix coiled protein, which is part of the valve basement membrane structure. (medscape.com)
This antibody was used in combination with anti-laminin-2 antibody C3_Hu10 to generate a duobody called TBTI (US11254737). (absoluteantibody.com)

A CMD Subtype that results from gene defects in the laminin-alpha2 gene affecting the production of merosin protein in the muscle cell. (cmdir.org)
Laminin is a protein that produces merosin for the extracellular matrix. (cmdir.org)

Crystal L. Abrams with her research poster "the effect of extracellular matrix protein laminin-1 on growth and differentiation of mouse embryonic stem cells" Crystal is a MEC RISE student. (cuny.edu)
EGF-like domains in extracellular matrix proteins: localized signals for growth and differentiation? (embl.de)

The laminins are an important and biologically active part of the basal lamina, influencing cell differentiation, migration, and adhesion. (pearltrees.com)
By a yeast two-hybrid approach, we identified an interaction between the AChE and an 898 bp fragment of laminin-beta1 chain, an extracellular matrix protein involved in neuronal differentiation and adhesion processes. (tu-darmstadt.de)
however, little is known about a role of laminin 411 in the regulation of IPC differentiation from human MSCs. (biomedcentral.com)
UC-MSCs were then cultured in the IPC induction and differentiation medium in the presence of laminin 411. (biomedcentral.com)
Laminin 411 markedly induced the expression of the genes Foxa2 and Sox17 , markers for pancreatic precursor cells, efficiently induced IPC differentiation from MSCs, and up-regulated insulin expression at both mRNA and protein levels. (biomedcentral.com)
Our results demonstrate that laminin 411 acts as a potent differentiation inducer of IPCs from UC-MSCs via the Pdx1 and Ngn3 signaling pathways. (biomedcentral.com)
SPARC (secreted protein,acidic and rich in cysteine) is a matricellular glycoprotein that regulates cell-cell and cell-matrix interactions, cellular proliferation and differentiation, and the expression of genes encoding extracellular matrix components. (silverchair.com)

The green and black tea extracts, EGCG, and theaflavins decreased the adherence of F. nucleatum to oral epithelial cells and matrix proteins. (nature.com)
T-cells that are responsive to the streptococcal M-protein infiltrate the valve through the valvular endothelium, activated by the binding of antistreptococcal carbohydrates with release or tumor necrosis factor (TNF) and interleukins. (medscape.com)
The laminins attach (bind) to other proteins in the extracellular matrix and in the membrane of muscle cells, which helps maintain the stability of muscle fibers. (medlineplus.gov)
To determine whether trabecular meshwork-inducible glucocorticoid response/myocilin (TIGR/MYOC) protein associates with the extracellular matrix (ECM) of human trabecular meshwork (HTM) cells. (nih.gov)
Protein adsorption is much more rapid than the transport of host cells to foreign surfaces. (nanomedicine.com)
Once proteins have adsorbed to the surface of the foreign material, host cells no longer see this underlying material, but only the protein-coated surface overlayer. (nanomedicine.com)
This adsorbed protein overlayer - rather than the foreign material itself - then mediates the types of cells that may adhere to the surface, which ultimately can determine the type of tissue that forms in the vicinity [ 517 ]. (nanomedicine.com)
Moreover, in addition to our understanding of the glycosylation of dystroglycan, much insight has also been gained into the functional roles of dystroglycan, not only in the bran and muscle, but also in other cells types, and the implication that the defective processing of this protein has with respect to role in Lassa fever virus infections. (enmc.org)
PGL-1 is involved in the M. leprae invasion of Schwann cells through the basal lamina in a laminin-2-dependent pathway. (ed.ac.uk)
The dominant expression of inwardly rectifying channels in cells from healthy donors is thought to support important glial cell functions such as the spatial buffering of extracellular K + . The downregulation of these channels and the less negative mean membrane potential in cells from patients should impair spatial buffering currents and neurotransmitter clearance. (arvojournals.org)
One of the main functions of Müller cells is the spatial buffering of the extracellular K + concentration in response to rises of neuronal activity. (arvojournals.org)
The extracellular matrix plays an important role in modulating the behavior of cells with which it interacts. (urosario.edu.co)
The lens capsule, which is also called the lens basement membrane, is a specialized extracellular matrix produced anteriorly by the lens epithelium and posteriorly by newly differentiated fiber cells. (silverchair.com)
Collagen receptors are cell surface receptors that modulate signal transduction between cells and the EXTRACELLULAR MATRIX. (lookformedical.com)
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules. (lookformedical.com)
Dystroglycan is also required non-cell-autonomously to organize the planar polarity of basal actin in follicle cells, possibly by organizing the Laminin ECM. (lu.se)
Matrix metalloproteases (MMPs) are zinc-dependent proteinases that participate in extracellular matrix degradation 1-3 and favor the invasion and proliferation of tumor cells 4 . (bvsalud.org)

Most LAMA2 gene mutations that cause the severe, early-onset form of LAMA2 -related muscular dystrophy result in the absence of functional laminin alpha-2 subunit. (medlineplus.gov)
Deficiency or absence of the laminin alpha-2 subunit results in a corresponding lack of laminin 2 and laminin 4, reducing the strength and stability of muscle tissue and leading to the signs and symptoms of LAMA2 -related muscular dystrophy. (medlineplus.gov)
The transmembrane protein Dystroglycan is a central element of the dystrophin-associated glycoprotein complex, which is involved in the pathogenesis of many forms of muscular dystrophy. (lu.se)
article{5f173e96-c6fe-4b5f-8f37-b070076bc387, abstract = {{The transmembrane protein Dystroglycan is a central element of the dystrophin-associated glycoprotein complex, which is involved in the pathogenesis of many forms of muscular dystrophy. (lu.se)
Gawlik KI, Harandi VM, Cheong RY, Petersén Å, Durbeej M. Laminin α1 reduces muscular dystrophy in dy2J mice. (lu.se)

Other than water, the mesoglea is composed of several substances including fibrous proteins like collagen and heparan sulphate proteoglycans. (pearltrees.com)
Lamins, also known as nuclear lamins are fibrous proteins in type V intermediate filaments, providing structural function and transcriptional regulation in the cell nucleus. (cmdir.org)

Matriglycan is indeed a scaffold for multiple laminin-G (LG) domain-containing extracellular matrix proteins (e.g. laminin, agrin, and perlecan) and it has the remarkable capacity to be tuned during skeletal muscle development and regeneration. (enmc.org)

We conclude that whether peptidoglycan binding proteins do not appear to be major adhesins, most of the choline-binding proteins interact with host proteins (elastin and C reactive proteins are the major Cbp partners). (biomedcentral.com)

This gene provides instructions for making a part (subunit) of certain members of a protein family called laminins. (medlineplus.gov)
Mutations that cause the milder, later-onset form usually result in a reduction (deficiency) of functional laminin alpha-2 subunit. (medlineplus.gov)
The predominant form of the protein is comprised of two alpha1(IV) subunits and one alpha2(IV) subunit, however, at least six different alpha subunits can be incorporated into the heterotrimer. (lookformedical.com)

Non-specific cross reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible. (covalab.com)

PGL-1 binds specifically to the native laminin-2 in the basal lamina of Schwann cell-axon units. (ed.ac.uk)
Se localiza en la membrana basal de la lámina lúcida y funciona uniendo las células epiteliales a las membranas basales. (bvsalud.org)

We make use of a highly flexible multi-step cloning strategy in order to allow for the rapid synthesis of new recombinant proteins that can introduce many different biofunctionalities. (frontiersin.org)

Inhibition of proteoglycan biosynthesis was accompanied by an inhibition of laminin deposition into extracellular matrix as determined by immunostaining of cultures and by immunoblotting of cell-associated proteins. (rupress.org)

The extracellular localization of TIGR/MYOC was examined by immunofluorescence microscopy in HTM cultures treated with and without dexamethasone and ascorbate and in a transformed HTM cell line, TM-1, transiently transfected with TIGR/MYOC cDNA. (nih.gov)

Dystroglycan is a core and essential component of the dystrophin-glycoprotein complex of muscle and brain, making vital links between the cytoskeleton and laminin in the extracellular matrix. (enmc.org)

A non-fibrillar collagen that forms a network of MICROFIBRILS within the EXTRACELLULAR MATRIX of CONNECTIVE TISSUE. (lookformedical.com)
This classification was based on the notion that "collagen" was equivalent to "connective tissue", but with the present recognition of the different types of collagen and the aggregates derived from them as distinct entities, the term "collagen diseases" now pertains exclusively to those inherited conditions in which the primary defect is at the gene level and affects collagen biosynthesis, post-translational modification, or extracellular processing directly. (lookformedical.com)
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. (lookformedical.com)

The C-terminal domain is where most of the variation is found between species and contains different amino acids which allow interaction with specific proteins in extracellular matrix or on the cell surface. (wikipedia.org)
This wealth of information has led to a new understanding of how the defective interaction between of dystroglycan and laminin leads to disease. (enmc.org)
The yeast two-hybrid data indicated that the interaction between these two proteins is moderately strong and that the region containing 240th amino acid residue to 503th amino acid residues of AChE is essential for interaction. (tu-darmstadt.de)
Engineered constructs were compared to human SAS and assessed for specific cell-cell interaction markers as well as for extracellular matrix proteins found in human meninges. (biomedcentral.com)
They stained positively for specific cell-cell interaction markers indicative of a functional meningeal tissue, as well as extracellular matrix proteins found in human meninges. (biomedcentral.com)

the Beta-dystroglycan is a transmembrane protein that plays an important role in connecting the extracellular matrix to the cytoskeleton. (absoluteantibody.com)

One common example is fibrinogen, a molecule that is reduced slightly in molecular weight and ultimately converted to the active protein, fibrin, after foreign surface contact during blood clotting ( Section 15.2.5 ). (nanomedicine.com)

To gain an insight into the noncholinergic, cell adhesion promoting functions of acetylcholinesterase, we sought proteins interacting with it. (tu-darmstadt.de)
P-selectin, soluble endothelial protein C receptor, soluble thrombomodulin, plasminogen activator inhibitor 1, ADAMTS-13, von Willebrand factor, tissue factor, soluble intercellular adhesion molecule 1, and vascular cell adhesion molecule 1 were more elevated in LF patients than in controls. (cdc.gov)

The major structural protein of basement membrane, collagen type IV, in the lens capsule was substantially altered in the absence of SPARC. (silverchair.com)

Nuclear lamins interact with inner nuclear membrane proteins to form the nuclear lamina on the interior of the nuclear envelope. (cmdir.org)

Laminin 2 and laminin 4 play a particularly important role in the skeletal muscles. (medlineplus.gov)
This allows dystroglycan to function as the predominant extracellular matrix receptor in skeletal muscle and brain. (enmc.org)

Cross reacts with most mammalian proteins. (covalab.com)
A polypeptide substance comprising about one third of the total protein in mammalian organisms. (lookformedical.com)

Analysis by RNA sequencing revealed altered expression of genes associated with extracellular matrix remodeling, endo-lysosomal processing, and mitochondrial energy metabolism under pathophysiological flow conditions. (biomedcentral.com)

Perturbations in the ability of dystroglycan to interact with laminin have severe implications for human disease. (enmc.org)
2003). In the second part of the work, a yeast two-hybrid screen was initiated aimed at identifying novel proteins that interact directly with AChE. (tu-darmstadt.de)

A proposed model for netrin activity in the spinal column of developing human embryos is that netrins are released by the floor plate and then are picked up by receptor proteins embedded in the growth cones of axons belonging to neurons in the developing spinal column. (wikipedia.org)
Explore our comprehensive collection of recombinant Fc receptor proteins! (acrobiosystems.com)

3] They are composed of a family of related proteins sharing common structural and sequence features. (pearltrees.com)

Mostajeran M, Wetterling F, Blixt FW, Edvinsson L, Ansar S. Acute mitogen-activated protein kinase 1/2 inhibition improves functional recovery and vascular changes after ischaemic stroke in rat-monitored by 9.4 T magnetic resonance imaging. (lu.se)

This binding is mediated by the alpha(2LG1, alpha2LG4, and alpha2LG5 modules present in the naturally cleaved fragments of the peripheral nerve laminin alpha2 chain, and is inhibited by the synthetic terminal trisaccharide of PGL-1. (ed.ac.uk)
Synthetic peptides from the regions of adherence on human laminin. (covalab.com)
Convenient, ready-to-use polystyrene microplates are coated with extracellular matrix (ECM) proteins or synthetic polypeptides. (thomassci.com)

Using cell lines, we demonstrated that the levels of LAMC1 mRNA and protein were significantly higher in HCC cell lines than that in LO2 cell line. (jcancer.org)

Laminin 5 (Ln5) is an extracellular matrix protein that plays an important role in cell migration and tumor invasion. (spandidos-publications.com)
Laminins plays a major role in binding itself and other proteins together not only in the extracellular matrix but also to the muscle cell membrane. (cmdir.org)
The structure of a Ca(2+)-binding epidermal growth factor-like domain: its role in protein-protein interactions. (embl.de)
The crystal structure identifies the role of Ca2+ in maintaining the conformation of the N-terminal region of the domain, but more importantly demonstrates that Ca2+ can directly mediate protein-protein contacts. (embl.de)
The observed crystal packing of the domains provides a plausible model for the association of multiple tandemly linked EGF-like domains in proteins such as fibrillin-1, Notch, and protein S. This model is consistent with the known functional data and suggests a general biological role for these domains. (embl.de)
Multidomain proteins of the extracellular matrix (ECM) play an important role in development and maintenance of cellular organization and in tissue repair. (embl.de)
It plays an important role in linking dystrophin to the laminin-binding alpha-dystroglycan. (absoluteantibody.com)
Evidence suggests that the protein plays a role in tumor invasion. (bvsalud.org)

In addition, proteomic profile confirmed that a set of proteins, related to tumor aggressiveness, were positively affected by targeted nanoparticles. (dovepress.com)

Based on a better understanding of the basement membrane zone (BMZ) and the genes responsible for its components, newer treatments (eg, gene or protein therapy) may provide solutions to the skin fragility found in patients with epidermolysis bullosa. (medscape.com)

A 45-kilodalton protein heregulin-alpha (HRG-alpha) that specifically induced phosphorylation of p185erbB2 was purified from the conditioned medium of a human breast tumor cell line. (embl.de)
LAMC1 encodes an extracellular matrix protein, laminin γ1 chain, which is involved in several biological and pathological processes including tissue development, tumor cell invasion and metastasis. (jcancer.org)

Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. (covalab.com)

Later on, enzymatic processes strip away this small segment, releasing the molecule into an active extracellular substrate pool. (nanomedicine.com)
Since AChE is mostly an extracellular or membrane- attached molecule, the binding partners for AChE should be: expressed in the same tissues and at the same developmental stages as AChE, have both an extracellular and an intracellular domain connected through one or more transmembrane domains, and possibly be able to initiate an intracellular signaling cascade. (tu-darmstadt.de)
Endothelial protein C receptor, thrombomodulin, intercellular adhesion molecule 1, plasminogen activator inhibitor 1, D-dimer, and hepatocyte growth factor were higher in fatal than nonfatal LF cases. (cdc.gov)

In rare, isolated instances they involve primary mutations of dystroglycan itself, but more frequently are associated with mutations in genes involved in the post-translational modification of dystroglycan: the secondary dystroglycanopathies, but also the so-called tertiary dystroglycanopathies arising from mutations in metabolic enzymes and other proteins that are involved more generally in multiple glycosylation pathways and cellular roles that in turn also impinge on dystroglycan function. (enmc.org)
Furthermore, detailed biochemical and cell biological analyses have finely mapped the biosynthetic pathways leading to the glycosylation of dystroglycan that enable it to bind to laminin. (enmc.org)

Physical adsorption isotherms were experimentally determined for these engineered proteins, allowing for direct calculation of the available ligand density present on coated surfaces. (frontiersin.org)
Therefore, this engineered protein adsorption approach allows for the facile preparation of tunable, quantifiable, and reproducible surfaces for in vitro studies of cell-ligand interactions and for potential application as coatings on neural implants. (frontiersin.org)
Following the implantation of a biomaterial into a host tissue, the first event to occur at the tissue-material interface (which dictates biocompatibility) is the noncovalent adsorption of plasma proteins from blood onto the surface [ 517 - 520 , 936 , 954 ] if the material comes into direct contact with blood. (nanomedicine.com)

Netrins 1, 3, and 4 are secreted proteins, whereas G1 and G2 are membrane bound proteins tethered by Glycophosphatidylinositol tails. (wikipedia.org)
Netrins-G are secreted but remain bound to the extracellular surface of the cell membrane through Glycophosphatidylinositol (GPI). (wikipedia.org)
A common feature of all EGF-like domains is that they are found in the extracellular domain of membrane-bound proteins or in proteins known to be secreted (exception: prostaglandin G/H synthase). (embl.de)

Reacts with human Laminin. (covalab.com)

DCC and UNC-5 proteins mediate netrin-1 responses. (wikipedia.org)

Various diverse extracellular proteins possess Ca(2+)-binding epidermal growth factor (EGF)-like domains, the function of which remains uncertain. (embl.de)
This strongly suggested that the proteins encoded by these clones are binding partners of acetylcholinesterase in yeast. (tu-darmstadt.de)
Due to its subcellular localization, structure and functions, we first focused on showing that laminin is binding to AChE in another system than yeast. (tu-darmstadt.de)
Streptavidin is a tetrameric protein providing 4 high-affinity biotin binding sites. (acrobiosystems.com)
Availability of genome sequences facilitated the identification of pneumococcal surface proteins bearing characteristic motifs such as choline-binding proteins (Cbp) and peptidoglycan binding (LPXTG) proteins. (biomedcentral.com)

Die extrazelluläre Matrix von Bauchspeicheldrüsenkrebs ist sehr komplex und besteht aus vielen fibrotischen Proteinen, darunter Kollagene, Proteoglykane, Laminine und Fibronektin. (ipfdd.de)

The impaired homeostasis and platelet dysfunction implicate alterations in the protein C pathway, which might contribute to the loss of endothelial barrier function in fatal infections. (cdc.gov)

We designed a medium throughput approach to systematically test for interactions between these pneumococcal surface proteins and host proteins (extracellular matrix proteins, circulating proteins or immunity related proteins). (biomedcentral.com)

Over the past 20 years, the importance of proteins for S. pneumoniae virulence has become clear. (biomedcentral.com)

The protein expression of Ln5, PTEN, p-EGFR and p-Akt was assessed by immunohistochemical analysis, and their relationships to prognosis were analyzed. (spandidos-publications.com)
Protein expression of Ln5, p-EGFR and p-Akt was detected in 61.2 (60/98), 60.2 (59/98) and 45.3% (43/95) of patients with NSCLC, respectively. (spandidos-publications.com)
Furthermore, the expression of the genes known to govern insulin expression including Pdx1 and Ngn3 was markedly induced by laminin 411, which suggests that Pdx 1 and Ngn 3 signaling pathways are involved in laminin 411 induced-insulin expression machinery. (biomedcentral.com)

Anatomy3

Chemicals and Drugs10

Phenomena and Processes1

Fibronectin11

Collagens1

Major component of the basal lamina1

Molecules4

Thrombospondin2

Antibody2

Merosin2

Growth and differentiation2

Differentiation7

Cells17

Muscular dystrophy5

Fibrous proteins2

Agrin1

Elastin1

Subunit3

Antibodies1

Basal2

Recombinant proteins1

Deposition1

Microscopy1

Glycoprotein1

Connective tissue3

Interaction5

Cytoskeleton1

Fibrinogen1

Cell adhesion2

Collagen Type1

Membrane proteins1

Skeletal2

Mammalian2

Metabolism1

Interact2

Receptor proteins2

Structural1

Inhibition1

Synthetic3

MRNA and protein1

Role8

Aggressiveness1

Gene1

Tumor2

ELISA1

Molecule3

Dystroglycan2

Adsorption3

Bound3

Human1

Mediate1

Binding5

Laminine1

Pathway1

Systematically1

Virulence1

Expression3