Extracellular matrix fibroblasts focal adhesions. Medical search. Frequent questions

Our results demonstrate that cytoglobin expression is correlated with a more quiescent phenotype of stellate cells in culture and that cytoglobin is regulated by the extracellular matrix through integrin signalling dependent on activation of focal adhesion kinase. (biomedcentral.com)
Tenascin-C modulates matrix contraction via focal adhesion kinase- and Rho-mediated signaling pathways. (ox.ac.uk)
Using a synthetic model of the provisional matrix, we have found that tenascin-C regulates cell responses to a fibrin-FN matrix through modulation of focal adhesion kinase (FAK) and RhoA activation. (ox.ac.uk)
Defactinib is a selective inhibitor of focal adhesion kinase (FAK), which reduces stromal density in preclinical pancreatic cancer models and in liver metastases from pancreatic cancer patients. (pancan.org)
An adhesive phenotype is developed with actin stress fibers and activation of focal adhesion kinase (FAK) and Rho GTPase. (princeton.edu)
Focal adhesion Kinase (FAK) and Pyk2 are related protein-tyrosine kinases co-expressed in murine and human endothelial cells (ECs). (schlaepferlab.org)
FAK-null primary embryonic fibroblast proliferation in culture is blocked by p53 activation and released upon p53 or p21CIP1 cyclin dependent kinase inhibitor genetic inactivation (Fig. 2). (schlaepferlab.org)
RGD-independent cell adhesion to tTG-FN does not require transamidating activity, is mediated by the binding of tTG to cell-surface heparan sulfate chains, is dependent on the function of protein kinase Cα (PKCα) and leads to activation of the cell survival focal adhesion kinase (FAK). (ntu.ac.uk)
In the present study we identify the focal adhesion-associated proteins p130Cas, Pyk2, and paxillin as novel members of the tyrosine kinase signaling pathway downstream of CD36 and show that assembly of this complex is essential for microglial migration. (neuroprobe.com)
Furthermore, the p130Cas-interacting kinase Pyk2 and the cytoskeletal adapter protein paxillin also demonstrate CD36-dependent phosphorylation, identifying these focal adhesion molecules as additional members of this β-amyloid signaling cascade. (neuroprobe.com)
In experiments probing for a role of PKCa in human laminin-2-integrin-mediated cell adhesion and spreading of PC12 cells, we observed unexpected enhancements of adhesion, spreading and stress fiber formation to 1 mM Go¨6976 with concomitant increase in membrane translocation of PKCd and autophosphorylation of focal adhesion kinase (FAK). (mirnamimic.com)
Pharmacologic blockade of PKC inhibits cell adhesion and spreading as well as focal adhesion formation and focal adhesion kinase (FAK) phosphorylation [13,14]. (mirnamimic.com)
Focal Adhesion Kinase (FAK) and cofilin phosphorylation and RhoA Guanosine Triphosphatase (GTPase) activity Desmethyldoxepin HCl had been all improved in these spheroids in comparison to control acini. (healthweeks.com)
This was associated with dysregulation of cues from the ECM which is indicated by increased cell-cell and cell-ECM signaling through β1-integrin focal adhesion kinase (FAK) and RhoA. (healthweeks.com)

The particular type of intermediate filaments attached tothe desmosomes depends on the cell type: they are keratin filaments in mostepithelial cells, for example, and desmin filaments in heart muscle cells.The junction hasa dense cytoplasmic plaque composed of a complex of intracellular anchorproteins (plakoglobin and desmoplakin) that are responsible for connecting thecytoskeleton to the transmembrane adhesion proteins. (ostatic.com)
These adhesion proteins(desmoglein and desmocollin), like those at an adherens junction, belong to thecadherin family. (ostatic.com)
The transmembrane adhesion proteins in these cell-matrixjunctions are integrins a large family of proteins distinct from the cadherins.Focal adhesions enable cells to get a hold on the extracellular matrix through integrins that link intracellularly to actin filaments. (ostatic.com)
In the current study, we investigated the role of different extracellular matrix proteins in stellate cell proliferation, activation (alpha smooth muscle actin expression and retinoic acid uptake) and cytoglobin expression. (biomedcentral.com)
In particular the incorporation of small peptides derived from natural proteins can enable specific functions such as cell adhesion [26] and matrix degradation [27] and thereby improve TPEN cell-matrix interactions within PEG hydrogels. (cell-metabolism.com)
Thus, deposition of additional ECM components, expression of co-receptors for ECM, cleavage of adhesive proteins, and the architecture of the ECM microenvironment are different mechanisms for modulating cell responses to fibronectin matrix. (princeton.edu)
However, whether clustering of these molecular components into focal adhesions is actually required for these proteins to regulate cell motility is unclear. (korea.ac.kr)
Here we use quantitative microscopy to characterize descriptors of focal adhesion and cell motility for mouse embryonic fibroblasts and human fibrosarcoma cells, across a wide range of matrix compliance and following genetic manipulations of focal adhesion proteins (vinculin, talin, zyxin, FAK, and paxilin). (korea.ac.kr)
The predictive power of this relationship is comprehensively validated by disrupting nonfocal adhesion proteins (α-actinin, F-actin, and myosin II) and subcellular organelles (mitochondria, nuclear DNA, etc.) not known to affect either focal adhesions or cell migration. (korea.ac.kr)
As demonstrated on LF fibroblasts, the resistance to trypsin was similar in serum-supplemented and serum-free media, i.e., medium without cell adhesion-mediating proteins. (nature.com)
Based on all the results, the increased resistance to trypsinization of C2C12, LF, HaCaT, and VSMC cells on amine PPs can be explained most probably by a non-specific cell adhesion such as electrostatic interaction between the cells and amine groups on the material surface, rather than by the receptor-mediated adhesion through serum-derived proteins adsorbed on the PPs. (nature.com)
On the biochemistry function, POGLUT2 can catalyze to transfer the glucose from UDP-glucose to a serine residue and specifically target extracellular epidermal growth factor (EGF) repeats of proteins such as Notch signaling members (NOTCH1 and NOTCH3) ( 9 , 10 ), thereby affecting Notch signaling pathway ( 11 ). (frontiersin.org)
We prepared Titanium (Ti) and Ce-TZP/Al 2 O 3 disks with different surface roughness, namely, mirrored and machined surfaces, on which HGF-1 were cultured, and then the biological parameters of the cultured cells, including cell morphology and gene expression of focal adhesion proteins were assessed. (alliedacademies.org)
Solid tumors are comprised of a mix cells - fibroblasts, blood vessels, immune cells, as well as the accumulation of extracellular matrix proteins. (schlaepferlab.org)
These properties will act mediating the action of cell adhesion proteins, regulating cell behavior and causing tissue regeneration. (bvsalud.org)
To occur the adhesion of CAMs to the biomaterial, it is necessary their interaction with some components of extracellular matrix, such as proteoglycans, collagen and proteins 23 . (bvsalud.org)

HSCs express numerous cell surface receptors including both discoidin domain receptors (DDRs)-which are receptors for collagen I and many of the integrin subunits, which are a major family of cell surface receptors for extracellular molecules and play a critical role in many biological functions [ 7 , 10 ]. (biomedcentral.com)
Interaction with ECM is a major driver of HSC morphology [ 11 ], and signal transduction from integrin activation occurs at least partly through aggregation and subsequent activation of focal adhesion kinases (FAKs) by autophosphorylation of tyrosine 397 (Y397). (biomedcentral.com)
VICs attach to the surrounding ECM at focal adhesions through integrin-receptor binding. (cell-metabolism.com)
Our findings showed that fibroblasts respond to substrate rigidity by recruiting more force-bearing integrins and modulating integrin sampling frequency of the ECM, rather than simply overloading the existing integrin-ligand bonds, to promote focal adhesion maturation. (bvsalud.org)
Fibroblasts adhere to fibronectin in this matrix via concomitant engagement of α5β1 integrin receptors and syndecan-4, a transmembrane proteoglycan. (princeton.edu)
Single cell mesenchymal-type movement is characterized by an elongated cellular shape and again requires extracellular proteolysis and integrin engagement. (biomedcentral.com)
tTG-FN supports RGD-independent cell adhesion of different cell types and the formation of distinctive RhoA-dependent focal adhesions following inhibition of integrin function by competitive RGD peptides and function blocking antiintegrin antibodies α5β1. (ntu.ac.uk)
We propose a novel RGD-independent cell adhesion mechanism that promotes cell survival when the anti-apoptotic role mediated by RGD-dependent integrin function is reduced as in tissue injury, which is consistent with the externalisation and binding of tTG to fibronectin following cell damage/stress. (ntu.ac.uk)
Biological activities of laminin are mediated through binding to a subset of integrin family of cell adhesion receptors, a3b1, a6b1, a6b4, and a7b1 [2]. (mirnamimic.com)

POGLUT2 was mainly expressed in stromal cells as verified by StromalScore, ESTIMATEScore, ImmuneScore, and Tumor purity, and POGLUT2 was positively correlated with cancer-associated fibroblasts, macrophages, monocytes, and neutrophils in the tumor microenvironment. (frontiersin.org)
Cancer-associated fibroblasts (CAFs) play a vital role in cancer chemoresistance, but detailed understanding of the process particularly in chemoresistant lung cancer is lacking. (cdc.gov)

Overall, the data suggest that microtubules play an important role in regulating cell tensegrity (e.g. by counteracting tension within the f-actin cytoskeleton) and/or contractility (e.g. by sequestering Rho-GEF) in corneal fibroblasts within 3-D collagen matrices. (arvojournals.org)
This active organization of ECM fibers suggest the important role of mechanical forces generated by the contractile cytoskeleton, which is transferred to the ECM through focal adhesion complexes. (charite.de)
Vinculin is an actin-binding protein (ABP) that strengthens the connection between the actin cytoskeleton and adhesion complexes. (bvsalud.org)
Environmental signals from the extracellular matrix (ECM) are transmitted by cell surface receptors that connect to the actin cytoskeleton and to multiple intracellular signaling pathways. (princeton.edu)
Focal adhesions are large protein complexes organized at the basal surface of cells, which physically connect the extracellular matrix to the cytoskeleton and have long been speculated to mediate cell migration. (korea.ac.kr)
The cell shape is determined by the interplay between the dynamics of the actin cytoskeleton, cell adhesions and the cell membrane towards the extracellular surface. (nusl.cz)
A lager expansion of the cell area accompanied by a greater development of actin cytoskeleton and talin1, a greater adhesion strength of CTEs to each disk, and higher gene expression level of focal adhesion protein were observed with the mirror-polished surface than the mechanically polished surface both Ti and Ce-TZP/Al 2 O 3 disks. (alliedacademies.org)
Mirror-polished Ce-TZP/Al 2 O 3 might contribute to the success of implant therapy by its strong adhesion strength to gingival connective tissues with the developed focal contact-cytoskeleton complex. (alliedacademies.org)
Together, these data are the first to identify the signaling cascade that directly links CD36 to the actin cytoskeleton and, thus, implicates it in diverse processes such as cellular migration, adhesion, and phagocytosis. (neuroprobe.com)
Directed cell migration is a complex process that involves front-rear polarization, characterized by cell adhesion and cytoskeleton-based protrusion, retraction, and contraction of either a single cell or a cell collective. (ibecbarcelona.eu)

Cell adhesion, proliferation, and colonization were enhanced on those scaffolds functionalized with RGD-ELR, while differentiation was promoted on those with SNA15-ELR. (ibecbarcelona.eu)
The combination of both RGD and SNA15 into the same ELR stimulated cell adhesion, proliferation, and differentiation, although at lower levels than those for every single moiety. (ibecbarcelona.eu)
Further investigation on the amount and distribution of RGD and SNA15 moieties in ELRs could improve cell adhesion, proliferation, and differentiation compared to the present study. (ibecbarcelona.eu)
We examined the correlation between nuclear deformation and cell functions such as cell proliferation, transfection and extracellular matrix protein type I collagen production. (cdc.gov)
The TGF-β family forms an important group of growth factors, consisting of three isoforms in man, and is important for matrix deposition because it modulates fibroblast recruitment and proliferation. (biomedcentral.com)
Our previously-obtained impressive results of highly increased C2C12 mouse myoblast adhesion to amine plasma polymers (PPs) motivated current detailed studies of cell resistance to trypsinization, cell proliferation, motility, and the rate of attachment carried out for fibroblasts (LF), keratinocytes (HaCaT), rat vascular smooth muscle cells (VSMC), and endothelial cells (HUVEC, HSVEC, and CPAE) on three different amine PPs. (nature.com)
This is controlled during the cell adhesion procedure through molecules and signalling pathways following a biological cascade, including proliferation and Extracellular Matrix (ECM) production [ 9 ]. (alliedacademies.org)

This results in a severe blistering of the skin, withleakage of body fluids into the loosened epithelium.Anchoring Junctions Formed by Integrins Bind Cells to theExtracellular Matrix: Focal Adhesions andHemidesmosomesSome anchoring junctions bind cells to the extracellular matrix rather than toother cells. (ostatic.com)
It binds to ß-catenin/N-cadherin complexes in apical adherens junctions (AJs), which maintain cell-to-cell adhesions, and to talin/integrins in the focal adhesions (FAs) that attach cells to the basal membrane. (bvsalud.org)
Fibronectin matrices have distinct effects on two other receptors: α4β1 and αvβ3 integrins. (princeton.edu)
FAK is best known for co-localization with integrins at cell adhesion sites to facilitate cell migration, mechano-sensing, and cell survival signaling (Fig. 1). (schlaepferlab.org)
Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. (modellab.cn)
Several molecules are involved in the process of cell adhesion to bone grafts, highlighting the role of integrins, the focal adhesion mechanism, the influence of the collagen matrix, and the activity of alkaline phosphatase in bone matrix formation. (bvsalud.org)

Collagen scaffold seeded with human dermal fibroblasts. (charite.de)
They treated human dermal fibroblasts from healthy individuals and from participants with diffuse cutaneous SSc with or without TGFβ, with or without celastrol. (rarediseaseadvisor.com)
Study findings reported that in dermal fibroblasts, celastrol weakened the power of TGFβ to generate an SSc-like pattern of gene expression, such as the cellular communication network factor 2, TGFβ1, and collagen I. Further, celastrol lessened the persistent fibrotic phenotype of dermal fibroblasts that had been cultured from the cells of patients with SSc. (rarediseaseadvisor.com)
The tTG-FN complex can maintain cell viability of tTG-null mouse dermal fibroblasts when apoptosis is induced by inhibition of RGD-dependent adhesion (anoikis), suggesting an extracellular survival role for tTG. (ntu.ac.uk)

The four panels represent roughly the different steps in the formation and disassembly of focal adhesions. (mechanobio.info)
Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). (rndsystems.com)

Although nanotopography has been shown to be a potent modulator of cell behavior, it is unclear how the nanotopographical cue, through focal adhesions, affects the nucleus, eventually influencing cell phenotype and function. (cdc.gov)
Our study delineated the relationships between focal adhesions, nucleus and cell function and highlighted that the nanotopography could regulate cell phenotype and function by modulating nuclear deformation. (cdc.gov)
One phenotype has quiescent fibroblast characteristics whereas the other has characteristics of myofibroblasts staining positively for smooth muscle mass alpha-actin (α-SMA) and associated with the remodeling of extracellular matrix (ECM). (cell-metabolism.com)
Many different research established that signaling through the extracellular matrix (ECM) includes a profound influence on gene appearance and mobile phenotype [1-4]. (healthweeks.com)

Osteonectin also increases the production and activity of matrix metalloproteinases, a function important to invading cancer cells within bone. (wikipedia.org)
Fibrosis is a physiological response to cellular injury in the liver and is mediated by the activation of hepatic stellate cells resulting in the replacement of hepatocytes with extracellular matrix comprised principally of collagen 1 to form a hepatic scar. (biomedcentral.com)
HSCs undergo phenotypic changes from quiescent vitamin A storing cells, involved in maintenance of the normal basement matrix, into proliferative myofibroblast-like cells, expressing myogenic markers such as alpha smooth muscle actin (αSMA). (biomedcentral.com)
Cells sense and respond to the mechanics of their substrate - be it the extracellular matrix, neighboring cells or artificial matrix in cell culture. (5dok.org)
We have therefore investigated the role of matrix stiffness in Notch signaling in breast cancer cells. (5dok.org)
This matrix serves as a scaffold for fibroblast migration into the wound where these cells deposit new matrix to replace lost or damaged tissue and eventually contract the matrix to bring the margins of the wound together. (ox.ac.uk)
Cells on fibrin-FN+tenascin-C redistribute their actin to the cell cortex, downregulate focal adhesion formation, and do not assemble a FN matrix. (ox.ac.uk)
Cells surrounded by a fibrin-FN+tenascin-C matrix are unable to induce matrix contraction. (ox.ac.uk)
The purpose of this study was to investigate the role of microtubules in modulating corneal fibroblast structure and mechanical behavior using 4-dimensional imaging of cells in 3-D culture. (arvojournals.org)
At 3 hours, focal adhesions were not well developed, microtubules were weakly labeled, and little matrix compaction was observed surrounding the cells. (arvojournals.org)
In contrast, at 24 hrs cells had well organized microtubules and prominent focal adhesions, and significant cell-induced matrix compaction was observed. (arvojournals.org)
Healing begins with the self-organisation of cells in the wound to reestablish structured tissue and restore the mechanical stability and intrinsic pretension of the injured matrix lost through the injury. (charite.de)
Our aim is to decipher this independent organisation of fibroblasts, vascular precursors, immune cells and mechanical instability in the complex environment of the tissue. (charite.de)
Cells are actively organizing their extracellular matrix (ECM). (charite.de)
The specific amounts of RGDS and PEG-PQ within the producing hydrogels influenced the elongation extracellular matrix deposition and hydrogel degradation behavior of encapsulated valvular interstitial cells (VICs). (cell-metabolism.com)
The biological function of the scaffold which plays a key role in the conversation between these resident cells and their surrounding ECM including both matrix degradation and new ECM deposition is usually another essential design feature that must be replicated within a TEHV [19]. (cell-metabolism.com)
Aggressive, transformed or transfected mesenchymal cells containing proto-oncogenes can act in the absence of lymphocytes, but whether these cells represent regressed fibroblasts, chondrocytes or bone marrow stem cells is unclear. (biomedcentral.com)
Cells interact with the extracellular matrix (ECM) via cell-ECM adhesions. (bvsalud.org)
The pancreatic cancer stroma is made up of fibroblasts and dense extracellular matrix, which are thought to limit the penetration of cancer-fighting drugs and T-cells, which may elicit an immune attack, from the tumor. (pancan.org)
Complimentary to this, fibroblasts exposed to fluid shear stress structurally rearranged pre-coated surface FN, but unstimulated and stretched cells did not. (nature.com)
The increased cell adhesion was also confirmed for LF cells by an independent technique, single-cell force spectroscopy. (nature.com)
Cell adhesion and spreading on the extracellular matrix is a morphogenetic process in which cells initially spread isotropically from the point of first contact and then spontaneously break their radial symmetry and develop a migratory polarity with spatially separated protruding cell front and non-protruding cell rear. (nusl.cz)
The resistance of cells on a biomaterial to compressive, tensile, or shear forces largely depends on the cell adhesion strength [ 8 ]. (alliedacademies.org)
In patients with diffuse cutaneous systemic sclerosis (SSc), inhibitors such as the Tripterygium wilfordii derivative celastrol have been shown to block the ways in which cells perceive matrix stiffness and thus may be beneficial in alleviating skin fibrosis. (rarediseaseadvisor.com)
Collective motility leads to movement of cohorts of cells which maintain the adherens junctions and move by photolytic degradation of matrix barriers. (biomedcentral.com)
For instance, MAPK inhibition in various varieties of tumor cells dependent on mutant RTKs or KRAS, such as for example EGFR (NSCLC), ABT-263 (Navitoclax) HER2 (breasts tumor) or ALK (neuroblastoma), can provoke a rise within the secretion of fibroblast development interleukin-6 and element. (bibf1120.com)
The tumor microenvironment (TME) includes an extracellular matrix and various varieties of regular cells, including tumor connected fibroblasts (CAFs), myofibroblasts, inflammatory cells, endothelial cells, pericytes and dendritic cells. (bibf1120.com)
A tendon is made of dense regular connective tissue , whose main cellular components are special fibroblasts called tendon cells (tenocytes). (handwiki.org)
Tendon cells synthesize the tendon's extracellular matrix , which abounds with densely-packed collagen fibers. (handwiki.org)
SPARC is produced by fibroblasts, capillary endothelial cells, platelets and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). (rndsystems.com)
PKC activation by phorbol myristate acetate has been shown to induce the adhesion, spreading, and migration of cells [12]. (mirnamimic.com)
These cell adhesion molecules (CAM) will mediate the contact between two cells or between cells and the extracellular matrix, an essential process to the success of the implant. (bvsalud.org)
Some of these molecules are glycoproteins expressed on the cell surface, socalled cell adhesion molecules (CAM), which mediate either the contact between two cells or the contact among cells and the extracellular matrix, being therefore of fundamental importance for adhesion 3 . (bvsalud.org)

The development of advanced scaffolds that recapitulate the anisotropic mechanical behavior and biological functions of the extracellular matrix in leaflets TPEN would be transformative for heart valve tissue engineering. (cell-metabolism.com)
Extracellular matrix (ECM) rigidity serves as a crucial mechanical cue impacting diverse biological processes. (bvsalud.org)
Although cell-ECM interactions have been studied extensively, it is not completely understood how immature (nascent) adhesions develop into mature (focal) adhesions and how mechanical forces influence this process. (bvsalud.org)
It was our objective to study the role of mechanical stimulation on fibronectin (FN) reorganization and recruitment by exposing fibroblasts to shear fluid flow and equibiaxial stretch. (nature.com)
This study aimed to investigate the mechanical adhesion strength of the Connective Tissue Equivalents (CTEs) to the Ceria-stabilized Tetragonal Zirconia Polycrystal/Alumina (Ce-TZP/Al 2 O 3 ) surface. (alliedacademies.org)
Furthermore, the mechanical adhesion strength of CTEs to the disk surface was measured using the nano-scratch test. (alliedacademies.org)
This study provides the first evidence for the mechanical adhesion strength of CTEs to Ce-TZP/Al 2 O 3 and Ti surfaces. (alliedacademies.org)
Connective tissue equivalents, Mechanical adhesion strength, Nano-scratch test. (alliedacademies.org)

Likewise,when cultured fibroblasts migrate on an artificial substratum coated withextracellular matrix molecules, they also grip the substratum at focaladhesions, where bundles of actin filaments terminate. (ostatic.com)
The best-characterized molecules are Rho, which controls the stress fibers and focal adhesion formation, and Rac and Cdc42, which regulate membrane ruffling, and filopodium formation, respectively. (biomedcentral.com)
Rho GTPases are key integrating molecules from different extracellular signals, as they can be activated by different GEFs. (biomedcentral.com)
In order to be effective and integrated to the receiving area, the bone graft is required to allow a strong cell adhesion, interacting with several molecules to induce migration, differentiation, and thus the mineralization of the new bone on the graft. (bvsalud.org)
This paper is a systematic review of the literature on the mechanisms of cell adhesion to bone grafts associated to nanotechnology, describing the importance and the role of those molecules in the adhesion and thus in tissue regeneration. (bvsalud.org)
The main reported mechanisms involve cell adhesion molecules (CAMs) and extracellular matrix components. (bvsalud.org)
Notwithstanding, to achieve the effectiveness and integration of the grafted tissue to the receptor site, it is necessary a strong cell adhesion, so that it demands many molecules interaction to induce cell differentiation and the bone matrix mineralization formed onto the graft. (bvsalud.org)

The effects of tenascin-C combined with its location around the wound bed suggest that this protein regulates fundamental processes of tissue repair by limiting the extent of matrix deposition and contraction to fibrin-FN-rich matrix in the primary wound area. (ox.ac.uk)
Development of the non-protruding regions requires the scaffold protein RACK1, which promotes adhesion-mediated activation of ERK2. (nusl.cz)
p53 effects can be mediated through alterations in matrix protein secretion or gene expression driving elevated cytokine factors altering immune surveillance and cell senescence. (schlaepferlab.org)
The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1‑5). (rndsystems.com)
Lipid-specific oligomerization of the Marburg virus matrix protein VP40 is regulated by two distinct interfaces for virion assembly. (uci.edu)
MARV assembles and buds from the host cell plasma where MARV matrix protein (mVP40) dimers associate with anionic lipids at the plasma membrane inner leaflet and undergo a dynamic and extensive self-oligomerization into the structural matrix layer. (uci.edu)
We demonstrate this behaviour on substrates coated with the cell-cell adhesion protein E-cadherin and then establish its generality on substrates coated with extracellular matrix. (ibecbarcelona.eu)

Synthetic peptides or recombinant fragments containing bio- logically active motifs have been shown to partially mimic the effects of native laminin in various cellular functions, such as cell adhesion, spreading, migration, neurite outgrowth, and tumor metastasis [6-10], suggesting that laminin-derived-short peptide- modified biomaterials may have therapeutic potential in tissue regeneration applications. (mirnamimic.com)

This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation of complement or the coagulation cascade and adhesion of leukocytes and platelets leading to thrombus formation. (ibecbarcelona.eu)
Compared with an unmodified glass surface, the grafting of polymer brushes minimizes the adhesion of platelets and leukocytes and prevents the thrombus formation. (ibecbarcelona.eu)

It was found that the nanoscale gratings and pillars could facilitate focal adhesion elongation by providing anchoring sites, and the nanogratings could orient focal adhesions and nuclei along the nanograting direction, depending on not only the feature size but also the spacing of the nanogratings. (cdc.gov)

Therefore FN matrix assembly depends on the precise coordination and synergy between extracellular events and intracellular pathways. (nature.com)

Focal adhesions act as molecular clutches that provide grip to the substrate for the lamellipodium to protrude forward during motility. (mechanobio.info)
Our study demonstrates a physical mechanism of collective durotaxis, through both cell-cell and cell-substrate adhesion ligands, based on the wetting properties of active droplets. (ibecbarcelona.eu)

Rabbit and human corneal fibroblasts transfected to express GFP-zyxin (to label focal adhesions) or GFP-tubulin (to label microtubules) were plated at low density inside 100 µm thick type I collagen matrices. (arvojournals.org)

Macrophages secrete matrix metalloproteinase 9 (MMP-9), an enzyme that weakens the fibrous cap of atherosclerotic plaques, predisposing them to plaque rupture and subsequent ischemic events. (neuroprobe.com)

A provisional matrix consisting of fibrin and fibronectin (FN) is deposited at sites of tissue damage and repair. (ox.ac.uk)
These observations show dual requirements for both FAK and RhoA activities during contraction of a fibrin-FN matrix. (ox.ac.uk)
To dissect how the ECM regulates cell functions, we are using a three-dimensional (3D) fibrin-fibronectin matrix, resembling the wound provisional matrix. (princeton.edu)
Although α4β1 does not naturally support strong cell interactions with a fibrin-fibronectin matrix, binding is dramatically enhanced by proteolytic cleavage of fibronectin. (princeton.edu)

In panel 2, some adhesions attach to stress fibers and grow in size while some disassemble (yellow with dotted outline) at the lamellipodium-lamellum boundary. (mechanobio.info)

Together with dynactin, dynein regulates centrosomal orientation to establish and maintain cell polarity, controls focal adhesion turnover and anchors microtubules at the leading edge. (biologists.com)

Our results show that we can tune cell stiffness and migration by regulating Notch activity and matrix stiffness. (5dok.org)

These differentially expressed lncRNAs are mainly involved in the biological process of the immune response and are closely related to the ECM-receptor interaction, MAPK signaling pathway, Focal adhesion, Ras signaling pathway, Antigen processing and presentation, and Chemokine signaling pathway. (biomedcentral.com)

From left: Panel 1 shows formation of nascent adhesions at the cell periphery. (mechanobio.info)

In preliminary experiments examining the role of PKCa in cell adhesion and spreading, we found that Go¨6976, a widely used, nonglycosidic indolocarbazole inhibitor of PKCa/b, had effects that could not be explained by inhibition of PKCa/b alone. (mirnamimic.com)

This growth factor also stimulates production of collagens, proteoglycans, elastin, fibronectin, tenascin and thrombospondin, diminishes production of extracellularly active neutral endoproteinases belonging to the matrix metalloproteinase (MMP) and serine proteinase families, and stimulates production of endogenous MMP inhibitors (tissue inhibitor of metallo-proteinase [TIMP]) and serpins (plasminogen activator inhibitor-1). (biomedcentral.com)
Specific association of tissue transglutaminase (tTG) with matrix fibronectin results in the formation of an extracellular complex (tTG-FN) with distinct adhesive and prosurvival characteristics. (ntu.ac.uk)

The protrusions of the cell edge are shown as green arrows corresponding to points of adhesion maturation (panels 3 and 4). (mechanobio.info)

Some cytokines act as competence rather than progression factors, some lack secretory signals, and some must be processed and released from the pericellular matrix or basement membranes (eg transforming growth factor beta [TGF-β ] binding to chondroitin or the keratan sulfate of biglycan, decorin and fibromodulin, or basic fibroblast growth factor and platelet derived growth factor binding to the heparin sulfate of glypican, perlecan and syndecan). (biomedcentral.com)

A specific inhibitor of PKCd, rottlerin, completely abrogated Go¨6976-dependent increase in PC12 cell adhesion to laminin as well as the activation of small GTPases, Rac1 and Cdc42, that are downstream of PKCd in adhesion receptor signaling. (mirnamimic.com)

Tenascin-C is expressed transiently during wound repair in tissue adjacent to areas of injury and contacts the provisional matrix in vivo. (ox.ac.uk)

Cellular studies of NTD and CTD oligomerization interface mutants demonstrate the importance of each interface in matrix assembly. (uci.edu)

Osteonectin is an acidic extracellular matrix glycoprotein that plays a vital role in bone mineralization, cell-matrix interactions, and collagen binding. (wikipedia.org)

We, herein, engineered nanotopographies of various shapes (gratings and pillars) and dimensions (feature size, spacing and height), and thoroughly investigated cell spreading, focal adhesion organization and nuclear deformation of human primary fibroblasts as the model cell grown on the nanotopographies. (cdc.gov)
Compared with continuous nanogratings, discrete nanopillars tended to disrupt the formation and growth of focal adhesions and thus had less profound effects on nuclear deformation. (cdc.gov)

Osteonectin is also detectable in osteoid, bone matrix proper, and dentin. (wikipedia.org)
After the use of search strategies, 18 articles that describe processes of cell adhesion to bone grafts were selected. (bvsalud.org)
Accurate identification of these mechanisms of cell adhesion is essential for further advancement in tissue engineering, such as the production of biological bone substitutes that achieve a better clinical outcome. (bvsalud.org)

Throughout cell-material interaction, adhesion is influenced by the features of a biomaterial surface, comprising topography and physicochemical properties. (alliedacademies.org)

Anatomy40

Organisms6

Diseases6

Chemicals and Drugs133

Analytical, Diagnostic and Therapeutic Techniques and Equipment25

Phenomena and Processes43

Disciplines and Occupations1

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Kinase14

Proteins16

Integrin9

Cancer-associated fibroblasts2

Cytoskeleton10

Proliferation7

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Dermal fibroblasts4

Disassembly2

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Cells33

Mechanical8

Molecules7

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Interaction1