Loading...
  • currents
  • Glutamate, at concentrations within normal plasma levels, positively modulates K v 1.3 channel gating, causing currents to activate faster and at significantly more hyperpolarized potentials, hence rendering the T lymphocyte readily responsive to immune stimuli. (aspetjournals.org)
  • Extracellular acidosis evoked transient inward currents in glomus cells that were evident at pH 7.0 and half-activated (pH 50) at 6.3. (ahajournals.org)
  • For α7 receptors, agonist-evoked single-channel currents appear mainly as isolated brief openings (τ o = 0.35 ms), whereas macroscopic currents after a step pulse of agonist desensitize rapidly (τ d = 0.4 ms). In contrast for 5-HT 3A receptors, agonist-evoked single-channel currents appear as clusters of many long openings in quick succession (τ cluster = 1.2 s), whereas macroscopic currents desensitize slowly (τ d = 1.1 s). (jneurosci.org)
  • Simulations of simultaneous inhibition of repolarizing currents by anti-SSA/Ro Ab-positive sera showed the predominance of the HERG channel in controlling action potential duration and the QT interval. (unisi.it)
  • molecular
  • In this study, we investigated the possible molecular mechanisms of carotid body pH-sensing by recording the responses of glomus cells isolated from rat carotid body to rapid changes in extracellular pH using whole-cell patch-clamping technique. (ahajournals.org)
  • current
  • A polycystin-1 mutant lacking approximately 2900 amino acids of the extracellular region is targeted to the cell surface but does not produce current. (nih.gov)
  • In the current clamp mode extracellular acidosis evoked both a transient and sustained depolarization. (ahajournals.org)
  • According to the results of the present study it was concluded that TtT/GF cells possessed outward K^+ channels, characteristics of which were similar to those of the K^+ channels in glial and Schwann cells, and that the amplitude of the K^+ current in TtT/GF cells appeared to be regulated by the condition of the cell contact. (nii.ac.jp)
  • Application of PSK to DUM neurons attenuated the spiking frequency (EC50=11pM) due to reduction of a pacemaker Ca 2+ current through cAMP-inhibited pTRPγ channels. (frontiersin.org)
  • calcium
  • This channel gates in response to many irritant and proalgesic agents, and can be modulated by calcium and depolarizing voltage. (frontiersin.org)
  • Whereas
  • Thus, whereas CRACs mediate the Ca 2+ influx, K + channels act as the regulator of the amplitude and duration of the Ca 2+ signal. (aspetjournals.org)
  • Response amplitude depends on the number, probability of opening, and conductance of neurotransmitter-activated channels, whereas response lifetime depends on the stability of the open channel together with the probability the channel will reopen after a closing. (jneurosci.org)
  • cells
  • Co-expression of a loss-of-function mutant of polycystin-2 in CHO cells does not reduce polycystin-1-dependent channel activity. (nih.gov)
  • Recently, pH-sensitive ion channels have been described in glomus cells that respond directly to extracellular acidosis. (ahajournals.org)
  • Publications] Yasufuku-Takano J.et al: 'Heterzbygous gsp mutation renders ion channels of human somatotroph adenoma cells weresponsive to GHRH' Endocrinology. (nii.ac.jp)
  • neurons
  • Thus, the satiety signal conferred by PSK acts antagonistically to the hunger signal, provided by the adipokinetic hormone (AKH): PSK depresses the electrical activity of DUM neurons by inhibiting the pTRPγ channel that is activated by AKH under conditions of food shortage. (frontiersin.org)
  • amiloride
  • It averaged 40.7±15.7 pA (n=5) at pH 5.0 and was blocked by the ASIC channel blocker amiloride (200 μm) to 2.5±1.6 pA. (ahajournals.org)
  • However, the sustained depolarization was not blocked by amiloride but was eliminated by removal of K + from the pipette solution which reduced significantly intracellular K + . This sustained depolarization was partially blocked by the TASK channels blockers anandamide (from 14.9±1.6 mV to 9.3±2.2 mV at pH 6.0, n=5) and quinidine (from 27.5±2.2 mV to 11.3±2.3 mV at pH 6.0, n=3). (ahajournals.org)
  • receptors
  • Here we show that structural differences within the interface dividing extracellular and transmembrane domains of homomeric α7 and 5-HT 3A receptors account for the large differences in open-channel lifetime and time of desensitization onset between these contrasting members of the Cys-loop receptor superfamily. (jneurosci.org)
  • The results suggest that desensitization is the major determinant of the lifetimes of activated α7 and 5-HT 3A receptors and that functional differences between the two receptors arise primarily through structural differences at the interface between extracellular and transmembrane domains. (jneurosci.org)
  • Thus, among different classes of synaptic receptors, diverging mechanisms determine open-channel lifetime. (jneurosci.org)
  • Guide to receptors and channels (GRAC) , 3rd edn (2008 revision). (wiley.com)
  • regulation
  • Publications] Yamasaki,T et al: 'Regulation of K^+ channels by cell contact in a cloned folliculo-stellate cell' Endocrinology. (nii.ac.jp)
  • gates
  • An important step to understanding ion channels is identifying the structural components that act as the gates to ion movement. (nih.gov)
  • structure
  • While the closed-state structure of TRPA1 has been recently resolved, also having its open state is essential for understanding how this channel works. (frontiersin.org)
  • experiments
  • Our best-in-class instruments facilitate the entire range of patch-clamp technique experiments from the smallest single-channel to the largest macroscopic recordings. (moleculardevices.com)
  • pathway
  • The results indicate that auxiliary beta subunits of BK channels reside sufficiently close to the ion permeation pathway defined by the alpha subunits to influence or block access of small molecules to the permeation pathway. (nih.gov)
  • found
  • This channel has also been found to be involved in the action of anaesthetics such as chloroform , halothane and propofol . (wikipedia.org)
  • single-channel
  • The Axopatch™ 200B Amplifier offers one of the lowest-noise, single-channel recordings available via an innovative actively cooled headstage with capacitor-feedback technology. (moleculardevices.com)