Extracellular cell derived microparticles. Medical search. Frequent questions

Lee, Y., El Andaloussi, S. & Wood, M. J. A. Exosomes and microvesicles: extracellular vesicles for genetic information transfer and gene therapy. (nature.com)
Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery. (nature.com)
Similar to other cell types, neutrophilic granulocytes also release extracellular vesicles (EVs), mainly medium-sized microvesicles/microparticles. (stanford.edu)
Microvesicles have a diameter ranging from 100 nm to 1 µm and are released by cell membrane budding. (spandidos-publications.com)
Our lab is also interested in extracellular vesicles such as exosomes and microvesicles as vehicles for the transfer of misfolded proteins between cells. (edu.au)
Extracellular vesicles (EV) are released by cells and include exosomes, microvesicles and microparticles. (edu.au)
ABSTRACT The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. (scienceopen.com)
The study of exosomes and other cell-derived microvesicles is an area of rapidly growing importance and the subject of intense interest and research. (malvernpanalytical.com)
Extracellular vesicular bodies such as microvesicles and exosomes are currently under intense investigation due to their apparently ubiquitous presence in a broad range of prokaryotic and eukaryotic organisms and the wide role they appear to play, at a fundamental level, in many biological processes, both physiological and pathological. (malvernpanalytical.com)

Here, we review recent progress in understanding extracellular vesicle biology and the role of extracellular vesicles in disease, discuss emerging therapeutic opportunities and consider the associated challenges. (nature.com)
Exosomes can transfer molecules from one cell to another via membrane vesicle trafficking, thereby influencing the immune system, such as dendritic cells and B cells, and may play a functional role in mediating adaptive immune responses to pathogens and tumors. (wikipedia.org)
On the other hand, the "exosome vesicle " is an extracellular particle released from the endosomal compartment of most eukaryotic cells. (frontiersin.org)
WJMSC-derived small extracellular vesicle enhance T cell suppression through PD-L1. (nature.com)
Vesicle flow cytometry using fluorescence-based detection of EVs has the potential to realize the potential of cell-derived membrane vesicles as functional biomarkers for a variety of applications. (nih.gov)
Microparticles (MPs) are a type of extracellular vesicle (EV) distinct from exosomes (which are smaller and generated inside vesicular bodies prior to secretion through the open cannicular system) and apoptotic bodies (larger fragments of late-stage apoptotic cells carrying fragmented DNA and protein). (unityhealth.to)
Strikingly, prevention of DLL-4-Notch1 interaction by a blocking antibody abolished platelet aggregation and extracellular vesicle shedding induced by thrombin. (elifesciences.org)

Consequently, there is a growing interest in clinical applications of EVs as biomarkers and therapies alike, prompting establishment of an International Society for Extracellular Vesicles (ISEV) and a scientific journal devoted to EVs, the Journal of Extracellular Vesicles. (wikipedia.org)
The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. (scienceopen.com)

Extracellular vesicles (EVs)/exosomes can satisfy these criteria ( Marcus and Leonard, 2013 ). (frontiersin.org)
Exosomes are membrane-bound extracellular vesicles (EVs) that are produced in the endosomal compartment of most eukaryotic cells. (wikipedia.org)
If the MVB fuses with the cell surface (the plasma membrane), these ILVs are released as exosomes. (wikipedia.org)
Since the size of exosomes is limited by that of the parent MVB, exosomes are generally thought to be smaller than most other EVs, from about 30 to 150 nanometres (nm) in diameter: around the same size as many lipoproteins but much smaller than cells. (wikipedia.org)
In the case of cancer cells, exosomes may show differences in size, shape, morphology, and canonical markers from their donor cells. (wikipedia.org)
Exosomes were first discovered in the maturing mammalian reticulocyte (immature red blood cell) by Stahl and group in 1983 and Johnstone and group in 1983 further termed 'exosomes' by Johnstone and group in 1987. (wikipedia.org)
Exosomes were shown to participate in selective removal of many plasma membrane proteins as the reticulocyte becomes a mature red blood cell (erythrocyte). (wikipedia.org)
The ILVs become exosomes if the MVB merges with the cell membrane, releasing the internal vesicles into the extracellular space. (wikipedia.org)
Exosomes contain various molecular constituents of their cell of origin, including proteins and RNA. (wikipedia.org)
Although the exosomal protein composition varies with the cell and tissue of origin, most exosomes contain an evolutionarily-conserved common set of protein molecules. (wikipedia.org)
The content of exosomes changes depending on the cells of origin, and they thereby reflect their originating cells. (wikipedia.org)
Therefore, scientists who are actively researching the role that exosomes may play in cell-to-cell signaling, often hypothesize that delivery of their cargo RNA molecules can explain biological effects. (wikipedia.org)
For example, mRNA in exosomes has been suggested to affect protein production in the recipient cell. (wikipedia.org)
However, another study has suggested that miRNAs in exosomes secreted by mesenchymal stem cells (MSC) are predominantly pre- and not mature miRNAs. (wikipedia.org)
Because the authors of this study did not find RNA-induced silencing complex-associated proteins in these exosomes, they suggested that only the pre-miRNAs, but not the mature miRNAs in MSC exosomes, have the potential to be biologically active in the recipient cells. (wikipedia.org)
Therefore, research on exosomes produced by MSC has been intensified for use in cell-free regenerative medicine. (frontiersin.org)
Exosomes are one of the most researched classes of extracellular vesicles because they are carriers of targeted protein and DNA/RNA loads. (spandidos-publications.com)
The profound roles of exosomes in homeostasis, stress and several pathological conditions, in conjunction with their selective and cell‑specific composition/function, allude to their use as promising circulating clinical biomarkers of systemic stress and specific pathologic states, and as biocompatible vehicles of therapeutic cargo. (spandidos-publications.com)
Exosomes are small lipid bilayer-surrounded extracellular vesicles released from cells into the extracellular space or biological fluids ( 1 , 2 ). (spandidos-publications.com)
Exosomes, via their cargo or surface composition, are signals/mediators of systemic homeostasis and stress for specific cell-to-cell or tissue-to-tissue communication ( 5 ). (spandidos-publications.com)
9. Fatima F, Nawaz M. Stem cell-derived exosomes: roles in stromal remodeling, tumor progression, and cancer immunotherapy. (ac.ir)
Mesenchymal stem cells-derived exosomes are more immunosuppressive than microparticles in inflammatory arthritis. (ac.ir)
13. Wang X, Omar O, Vazirisani F, Thomsen P, Ekström K. Mesenchymal stem cell-derived exosomes have altered microRNA profiles and induce osteogenic differentiation depending on the stage of differentiation. (ac.ir)
14. Yang Y, Bucan V, Baehre H, Von Der Ohe J, Otte A, Hass R. Acquisition of new tumor cell properties by MSC-derived exosomes. (ac.ir)
With the discovery of the antigen-presenting exosomes secreted by B lymphocytes involving anti-tumor responses in 1996 [1] and the exchange of genetic material between cells through RNA of the exosomes in 2007 [2] , people became aware of the importance of exosomes. (cusabio.com)
We are also using high-resolution cryo-electron microscopy to study the structure of exosomes isolated from neuronal cells and characterise their protein and RNA content using high throughput sequencing and proteomics techniques. (edu.au)
Membrane-associated Hsp72 from tumor-derived exosomes mediates STAT3-dependent immunosuppressive function of mouse and human myeloid-derived suppressor cells. (scienceopen.com)
Here, we have isolated tumor-derived exosomes (TDEs) from mouse cell lines and shown that an interaction between TDE-associated Hsp72 and MDSCs determines the suppressive activity of the MDSCs via activation of Stat3. (scienceopen.com)
2009) define exosomes as 40 -100 nm diameter membrane vesicles of endocytic origin that are released by most cell types upon fusion of multivesicular bodies with the plasma membrane, presumably as a vehicle for cell-free intercellular communication. (malvernpanalytical.com)
Mesenchymal stem cells in regenerative medicine applied to rheumatic diseases: role of secretome and exosomes. (anova-irm.com)

Moreover, both unmodified and engineered extracellular vesicles are likely to have applications in macromolecular drug delivery. (nature.com)

Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)
However, when employing MSC transplantation to treat tissue defects, the research found that: less than one percent of MSCs could home and colonize in the tissue defect area, continuously promoting tissue regeneration, and the paracrine mechanism of MSCs plays a more important role than the replacement of damaged cells by differentiation [ 13 ]. (biomedcentral.com)
Likewise, they have the ability to activate the angiogenesis, proliferation, migration, and differentiation of the main cell types involved in skin regeneration. (frontiersin.org)
Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
The posterior download drawing made easy dragons fantasy unleash your of self-containing die in reading beobachten stem and andere with Organ-derived Periodontal differentiation. (it-dresden.net)
EV-interactions and uptake induce changes in cellular proliferation, differentiation, cell movement, as well as transcriptional and epigenetic regulation. (trillium.de)
Thereby, functionally manipulating recipient cells, for example to perturb pro-inflammatory signalling, inhibit cellular proliferation, differentiation and to promote regeneration and repair in a broad range of pathological tissues. (trillium.de)
Analytes include soluble growth and differentiation factors, extracellular matrix components, proteases, membrane-bound receptors, and intracellular signaling molecules. (rndsystems.com)
Up-regulation of autophagy induces endothelial cell regeneration/differentiation and improves the function of impaired ones. (koreamed.org)
that critically influences cell proliferation, differentiation and apoptosis ( Miele and Osborne, 1999 ). (elifesciences.org)

Cutting-edge analysis of extracellular microparticles using ImageStream(X) imaging flow cytometry. (qmul.ac.uk)
Proteomic analysis of extracellular vesicles (EVs) from biological fluid is a powerful approach to discover potential biomarkers for human diseases including cancers, as EV secreted to biological fluids are originated from the affected tissue. (molcells.org)

The exosome vesicles are a type of extracellular vesicles (EV), which are defined as lipid-bilayer spheroid structures, without replicating capacity, that are released from cells, including both prokaryotes and eukaryotes. (frontiersin.org)

Flow cytometry is a popular tool for characterizing heterogeneous mixtures of particles such as cell types within blood, but the small size of EVs makes them difficult to measure using conventional flow cytometry. (nih.gov)

Exosome/microvesicle-mediated epigenetic reprogramming of cells. (nature.com)
Lai, R. C., Chen, T. S. & Lim, S. K. Mesenchymal stem cell exosome: a novel stem cell-based therapy for cardiovascular disease. (nature.com)
Thus, it may refer to the "exosome complex ," being a proteic (enzymatic) macromolecular machinery, present in archaea and eukaryotic cells, being involved in RNA degradation. (frontiersin.org)
16. Wang J, Zheng Y, Zhao M. Exosome-Based Cancer Therapy: Implication for Targeting Cancer Stem Cells. (ac.ir)
This project will also investigate the role of modifiers in exosome biogenesis by altering their expression with RNAi, using a lentiviral delivery system in neuronal cells. (edu.au)
MSC exosome as a cell-free MSC therapy for cartilage regeneration: Implications for osteoarthritis treatment. (anova-irm.com)

Kim and Park, 2017 ) and make HMSCs attractive cells for regenerative therapies. (frontiersin.org)
Frontiers in Cell and Developmental Biology 5 (2017). (anova-irm.com)

EVs in circulation carry genetic material and proteins from their cell of origin, proteo-transcriptomic signatures that act as biomarkers. (wikipedia.org)
According to published data, authors have reached a consensus on the physical parameters (size, density) and chemical composition (surface proteins, proteomics) of neutrophil-derived EVs. (stanford.edu)
CD3 for T-cell MPs, etc) and carry a complement of surface proteins, enzymes, signaling proteins, second messengers and RNA species known to regulate the function of host/target cells. (unityhealth.to)
We are interested in investigating the mechanisms, of how these proteins are packaged into these vesicles and also how they transfer their contents between cells. (edu.au)
EV surface proteins and lipids enable interaction with particular cells, whereas their internal payload of RNA, transcription factors, DNA, enzymes and metabolites functionally alters recipient cells. (trillium.de)
Varying Oxygen Partial Pressure Elicits Blood-Borne Microparticles Expressing Different Cell-Specific Proteins - Toward a Targeted Use of Oxygen? (daneurope.org)
In the study of cancer, EV secreted by cancer cells has been shown to contain biologically active proteins. (molcells.org)
By further exploring the biological cargo of these vesicles, a better understanding may emerge regarding the proteins employed by the cancer cells for propagation. (molcells.org)

This paper reviews current knowledge on the functional alterations initiated by neutrophil-derived EVs, listing their effects according to the triggering agents and target cells. (stanford.edu)
We summarize the presence of neutrophil-derived EVs in pathological processes and their perspectives in diagnostics and therapy. (stanford.edu)
Neutrophil extracellular traps (NETs) and neutrophil-derived microparticles express tissue factor after stimulation by ANCA, causing activation of coagulation (11). (researchsquare.com)

The mesenchymal stromal cells (MSC) have immunomodulatory properties and a high regenerative capacity. (frontiersin.org)
With the support of a Versus Arthritis Senior Fellowship I aim to establish how SPM reprogram innate immune cells and stromal cells within the joint tissue to switch on repair mechanisms that temper the aggressive and inappropriate behaviour of cells within the joint tissue to ultimately protect the cartilage from erosion. (qmul.ac.uk)
When administered post-stroke, mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) induce neuroprotection, promote brain remodeling and plasticity, and enhance neurological recovery in rodents and non-human primates via mechanisms that involve immunomodulation and anti-inflammation. (explorationpub.com)
Recently, mesenchymal stromal cell (MSC)-derived small extracellular vesicles (EVs) have emerged as a potential new therapeutic agent for stroke treatment. (explorationpub.com)
Human amniotic membrane and amniotic membrane-derived mesenchymal stromal cells (hAMSCs) have produced promising results in regenerative medicine, especially for the treatment of inflammatory-based diseases and for different injuries including those in the orthopedic field such as tendon disorders. (mdpi.com)
Treatment of a full-thickness articular cartilage defect in the femoral condyle of an athlete with autologous bone-marrow stromal cells. (anova-irm.com)

They are derived by a targeted mechanism from the cell endocytic compartment and are formed and stored within the intracellular multivesicular bodies (MVBs). (spandidos-publications.com)
Furthermore, they also succeeded in selectively introducing small molecules into intracellular organelles usually impossible at low concentrations (hundreds of pmol/L) as well as inducing cell death in targeted cells by concentrating anticancer active peptides into them at concentrations so low that they would not be conventionally effective (several tens of nmol/L). (asiaresearchnews.com)
it consists of the extracellular binding domain without the intracellular signaling domain. (hindawi.com)
Highly efficient intracellular transduction in three-dimensional gradients for programming cell fate. (open.ac.uk)

Human mesenchymal stem cells (HMSCs) are multipotent somatic stem cells that can be isolated from a variety of tissues such as the bone marrow, adipose tissue, and dental pulp. (frontiersin.org)
In periodontitis, EVs play a pivotal role in regulating the biological functions of both periodontal tissue cells and immune cells, as well as the local microenvironment, thereby promoting periodontal injury repair and tissue regeneration. (biomedcentral.com)
By performing local transplants, other non-periodontal-derived MSCs such as dental pulp stem cells (DPSCs), bone marrow stem cells (BMSCs), and adipose tissue-derived stem cells (ADSCs) also aid in the regeneration of periodontal tissue [ 12 ]. (biomedcentral.com)
Adipose tissue-derived mesenchymal stem cells (AD-MSC) show great potential to treat inflammatory and degradative processes in OA and have demonstrated paracrine effects in chondrocytes. (karger.com)
The multi‑dimensional nature of their roles in cellular homeostasis, cell‑to‑cell and tissue‑to‑tissue communication at the level of the organism, as well as their actions on the holobiome (intra‑/interspecies interaction), have garnered the interest of a large number of researchers. (spandidos-publications.com)
Endocrinology, by definition, focuses on homeostatic, and cell‑to‑cell and tissue‑to‑tissue communication mechanisms. (spandidos-publications.com)
8 nm).Ppy NPs effectively augment the conductivity, surface roughness, andthickness of BC composites despite reducing scaffolds' transparency.BC-Ppy composites were flexible (up to 10 mM Ppy), maintained theirintricate 3D extracellular matrix-like mesh structure in all Ppy concentrationstested, and displayed electrical conductivities in the range of nativecardiac tissue. (ibecbarcelona.eu)
I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
5. Ahn J-O, Coh Y-R, Lee H-W, Shin I-S, Kang S-K, Youn H-Y. Human adipose tissue-derived mesenchymal stem cells inhibit melanoma growth in vitro and in vivo. (ac.ir)
A recent prospective study of patients with AAV, who were enrolled during active disease and followed longitudinally, demonstrated that elevated microparticle tissue factor activity, and increased levels of anti-plasminogen antibodies in remission, were strong indicators of VTE (10). (researchsquare.com)
Extracellular vesicles (EV) are mediators of intercellular communication locally in tissue microenvironments and enable distal across organ communication between cells of the same origin and those from different sources. (trillium.de)
Migratory chondrogenic progenitor cells from repair tissue during the later stages of human osteoarthritis , Cell Stem Cell 4 (2009) 324-335. (anova-irm.com)
B.A. Jones, M. Pei, Synovium-Derived stem cells: a tissue-Specific stem cell for cartilage engineering and regeneration , Tissue Eng. (anova-irm.com)
P-selectin, soluble endothelial protein C receptor, soluble thrombomodulin, plasminogen activator inhibitor 1, ADAMTS-13, von Willebrand factor, tissue factor, soluble intercellular adhesion molecule 1, and vascular cell adhesion molecule 1 were more elevated in LF patients than in controls. (cdc.gov)
Pathologic changes seen on autopsy lack major cell and tissue injury but include signs of pleural effusion, pulmonary edema, ascites, and gastrointestinal mucosa bleeding ( 5 , 6 ), all indications of systemic vascular leakage. (cdc.gov)

The PD-L1 on tumor cell-derived small extracellular vesicles (sEVs) can suppress the proliferation and cytokine production of T cells. (nature.com)

Through them, the cells modify their microenvironment and the behavior of neighboring cells. (frontiersin.org)
Extracellular vesicles as modulators of the cancer microenvironment. (ac.ir)
How do mesenchymal stem cells influence or are influenced by microenvironment through extracellular vesicles communication? (anova-irm.com)

Literature survey indicated the origin of the miR from liver cells and not of platelet, leukocyte, smooth muscle or endothelial (EC) origin. (biomedcentral.com)
Patients with anti-β2GPI antibodies display significantly higher platelet activation/aggregation and vascular endothelial cell damage. (biomedcentral.com)
The mechanism underlying the correlation between platelet activation, vascular endothelial cell dysfunctions and anti-β2GPI antibodies remains unknown. (biomedcentral.com)
Anti-β2GPI/β2GPI complex induces the release of platelet-derived microparticles (p-MPs). (biomedcentral.com)
The targeting role of these miRNAs was further validated by directly downregulating targeted selectin-P (SELP) and platelet-derived growth factor receptor alpha (PDGFRA) via luciferase activity assay. (biomedcentral.com)
Our results here show that the anti-β2GPI/β2GPI complex induces the release of platelet microparticles and increases the level of miR-96 and -26a in platelet-derived microparticles. (biomedcentral.com)
Finally, selectin-P (SELP) and platelet-derived growth factor receptor alpha (PDGFRA) were identified as the targets of these two miRNAs. (biomedcentral.com)
Our study suggests that the anti-β2GPI/β2GPI complex regulates both platelets and vascular endothelial cells in the transfer of platelet-derived miRNAs. (biomedcentral.com)
Platelet-derived extracellular vesicles were documented with Nanoparticle Tracking Analyzer. (elifesciences.org)

Within the past decade, extracellular vesicles have emerged as important mediators of intercellular communication, being involved in the transmission of biological signals between cells in both prokaryotes and higher eukaryotes to regulate a diverse range of biological processes. (nature.com)

Additionally, for many other engineered or synthetic advanced therapies, the amount of therapeutic agent required to elicit an efficacious response is high, necessitating their conveyance in a larger nanoparticle or a microparticle much larger than an EV. (corning.com)
Osaka Metropolitan University researchers have used the power of laser light to accelerate the reaction between cancer cell-derived extracellular vesicles-a kind of nanoparticle-and antibody-modified microparticles. (asiaresearchnews.com)

MicroRNA-126 (miR-126) is endothelial cell (EC) specific and plays a key role in regulating EC function, controlling angiogenesis, and maintaining vascular integrity. (justia.com)
Reductions in uteroplacental flow initiate a cascade of molecular effects leading to hypoxia, thrombosis, inflammation, and endothelial cell dysfunction resulting in untoward pregnancy outcomes. (hindawi.com)
However, TM is a receptor of thrombin and protein C on the endothelial cell surface and regulates the coagulation and complement system [ 15 ]. (biomedcentral.com)
Soluble TM is formed via the limited proteolysis of TM by neutrophil elastase on the endothelial cell surface [ 16 , 17 ], but it has not been confirmed that soluble TM is actively secreted by endothelial cells. (biomedcentral.com)
Furthermore, soluble TM has only 20% of activity of normal TM on the endothelial cell surface [ 18 ]. (biomedcentral.com)
In this study, we derived miR-96 and -26a from platelets activated by the anti-β2GPI/β2GPI complex and explored their role in modulating human umbilical vein endothelial cell (HUVEC) migration and tube formation. (biomedcentral.com)
Damage to ECs is catastrophic and induces endothelial cell dysfunction. (koreamed.org)
Tree of life: endothelial cell in norm and disease, the good guy is a partner in crime! (koreamed.org)
Central role of metabolism in endothelial cell function and vascular disease. (koreamed.org)
EndoDB: a database of endothelial cell transcriptomics data. (koreamed.org)

Mesenchymal stem cells (MSCs) are multipotent cells with regenerative and immunomodulatory properties. (frontiersin.org)
Human mesenchymal stem cell-conditioned medium improves cardiac function following myocardial infarction. (nature.com)
Stem Cell Res. (nature.com)
Reduction of myocardial infarct size by human mesenchymal stem cell conditioned medium. (nature.com)
thus, due to the unique characteristics of mesenchymal stem cells (MSCs), they have become the focus of attention and may be the most promising new therapy for periodontitis. (biomedcentral.com)
MSCs are multipotent stem cells with the ability to self-renew and differentiate in multiple directions [ 3 ]. (biomedcentral.com)
The one that has arguably received the most attention from industry are what have been referred to as "naïve" EVs, or unmodified EVs produced from stem cells. (corning.com)
They are secreted by a wide array of cells, including cardiac myocytes, mature and progenitor endothelial cells, mesenchymal stem cells, immune cells like antigen-presenting cells (APCs), and malignant cells 5 , 6 . (bmrat.org)
My research continues to focus on stem cells and osteoblasts and in particular 3D cell culture models and microenvironments. (nottingham.ac.uk)
Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
Controversial results have been reported regarding the anti-tumor properties of extracellular vesicles derived from mesenchymal stem cells (MSCs). (ac.ir)
The present study was conducted to evaluate whether secretome derived from Human Wharton's jelly mesenchymal stem cells (hWJMSCs) may stimulate or inhibit breast cancer growth in vitro and in vivo. (ac.ir)
1. Usha L, Rao G, Christopherson II K, Xu X. Mesenchymal stem cells develop tumor tropism but do not accelerate breast cancer tumorigenesis in a somatic mouse breast cancer model. (ac.ir)
Mesenchymal stem cells derived from human adipose tissues favor tumor cell growth in vivo. (ac.ir)
4. Yang J, Lv K, Sun J, Guan J. Anti-tumor effects of engineered mesenchymal stem cells in colon cancer model. (ac.ir)
Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma. (ac.ir)
Human bone marrow mesenchymal stem cells display anti-cancer activity in SCID mice bearing disseminated non-Hodgkin's lymphoma xenografts. (ac.ir)
Naive human umbilical cord matrix derived stem cells significantly attenuate growth of human breast cancer cells in vitro and in vivo. (ac.ir)
10. Rani S, Ryan AE, Griffin MD, Ritter T. Mesenchymal stem cell-derived extracellular vesicles: toward cell-free therapeutic applications. (ac.ir)
A biomaterials approach to influence stem cell fate in injectable cell-based therapies. (open.ac.uk)
Stem Cell Research & Therapy , 9(1), article no. 39. (open.ac.uk)
Decellularized bone extracellular matrix and human dental pulp stem cells as a construct for bone regeneration. (open.ac.uk)
Extracellular matrix-derived hydrogels for dental stem cell delivery. (open.ac.uk)
Georg Hansmann, Philippe Chouvarine, Franziska Diekmann, Martin Giera, Markus Ralser, Michael Mülleder, Constantin von Kaisenberg, Harald Bertram, Ekaterina Legchenko & Ralf Hass "Human umbilical cord mesenchymal stem cell-derived treatment of severe pulmonary arterial hypertension" . (anova-irm.com)
Injectable mesenchymal stem cell therapy for large cartilage defects-a porcine model. (anova-irm.com)
Effect of intra-articular injection of autologous adipose-derived mesenchymal stem and regenerative cells on clinical signs of chronic osteoarthritis of the elbow joint in dogs. (anova-irm.com)
Increased knee cartilage volume in degenerative joint disease using percutaneously implanted, autologous mesenchymal stem cells. (anova-irm.com)
Intra-articular injection of autologous mesenchymal stem cells in six patients with knee osteoarthritis. (anova-irm.com)
Articular cartilage regeneration with autologous peripheral blood stem cells versus hyaluronic acid: a randomized controlled trial. (anova-irm.com)
Adult human mesenchymal stem cells delivered via intra-articular injection to the knee following partial medial meniscectomy. (anova-irm.com)
Mesenchymal stem cell therapy in the treatment of osteoarthritis: reparative pathways, safety and efficacy-a review. (anova-irm.com)

They are also released in vitro by cultured cells into their growth medium. (wikipedia.org)
It was verified that co-expression of CD80 disrupted the binding of sEV PD-L1 to its receptor PD-1 on T cells and attenuated the immunosuppression mediated by sEV PD-L1 both in vitro and in vivo. (nature.com)
Fig. 4: PD-L1-positive I-sEVs did not suppress T cells in vitro. (nature.com)
Her research interests include the preparation and design of materials and scaffolds for in vitro and in vivo fundamental studies, and a further focus is the provision of useful tools to assess mechanisms that govern cell behavior in regenerative medicine. (ibecbarcelona.eu)
In vitro experiments withcardiac fibroblasts and H9c2 cells confirmed the exceptional biocompatibilityof BC-Ppy composites. (ibecbarcelona.eu)
MTT assays was performed to determine anti-tumor effects of hWJMSCs-secretome on both MCF-7 and 4T1 tumor cells in vitro. (ac.ir)
We found that hWJMSCs-secretome can inhibit growth of MCF-7 and 4T1 tumor cell lines in vitro. (ac.ir)
These cytotoxic lymphocytes may be generated in vitro in mixed lymphocyte cultures (MLC), in vivo during a graft-versus-host (GVH) reaction, or after immunization with an allograft, tumor cell or virally transformed or chemically modified target cell. (edu.au)

Vaccination with human amniotic epithelial cells confer effective protection in a murine model of Colon adenocarcinoma. (ac.ir)
Microparticles/Microvessicles (~100-1000nm) are one of the more commonly studied populations and are typically induced following activation or inflammatory stimuli from many cell types including platelets, macrophages/monocytes, T-cells, B-cells, progenitor cells (EPCs, MSCs, etc.), cancer cells, endothelial and epithelial cells among many others. (unityhealth.to)

Microparticles are increasingly becoming realized as important biomarkers and mediators of disease. (unityhealth.to)

Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs. (nih.gov)
MPs are extremely small vesicles generated via off-budding from a parent cell. (unityhealth.to)

Extracellular vesicles act as a cell's transfer signals - these nanoparticles, which contain protein, sugars, and other biological materials, communicate with adjacent cells and tissues to transmit messages, such as whether a cell is healthy or in distress. (corning.com)
Small extracellular vesicles are non-replicating, nano-sized (70-150 nm) lipid bilayer-enclosed vesicles, which have shown remarkable biological activities in various physiological and pathophysiological contexts. (explorationpub.com)
Their multi‑functional cargo have been indicated to regulate a vast number of biological pathways in target cells. (spandidos-publications.com)
As such they have a plasma membrane bilayer and biological molecule endowment derived from a parent cell. (unityhealth.to)
Almost all cell types have been found to generate EVs in response to various biological stimuli. (unityhealth.to)

However, PD-L1-positive sEVs derived from immune cells did not exert immunosuppressive functions due to the co-expression of CD80. (nature.com)

PMP also differ from the microparticles derived from megakaryocytes despite the presence of several identical surface markers on the latter. (malvernpanalytical.com)
Notch signaling dictates cell fate decisions in mammalian cells including megakaryocytes. (elifesciences.org)

In addition, tumor-derived soluble factors triggered MDSC expansion via activation of Erk. (scienceopen.com)

However, PD-L1 can also be expressed by non-tumor cells. (nature.com)
PD-L1-positive sEVs in HNSCC patients had various cellular origins, including tumor cell, T cell, B cell, dendritic cell and monocyte/macrophage. (nature.com)
Fig. 3: Different clinical significance between tumor cell- and immunocyte-derived PD-L1-positive sEVs. (nature.com)
Tumor-associated B7-H1 promotes T-cell apoptosis: a potential mechanism of immune evasion. (nature.com)
Their small size confers other benefits, as well - too small to convey pathogens such as bacteria or viruses, EVs are also incapable of the unwanted replication or tumor formation that whole cell therapies can occasionally provoke. (corning.com)
We previously established that an unidentified factor, or factors, present in ovarian tumor ascites fluids reversibly inhibited the activation of T cells by arresting the T-cell signaling cascade. (aacrjournals.org)
Ultracentrifugation of the tumor ascites fluid has now revealed a pellet that contains small extracellular vesicles (EV) with an average diameter of 80 nm. (aacrjournals.org)
We also demonstrated that this mechanism is relevant in cancer patients, as TDEs from a human tumor cell line activated human MDSCs and triggered their suppressive function in an Hsp72/TLR2-dependent manner. (scienceopen.com)

sEVs were isolated from different clinical samples of head and neck squamous cell carcinoma (HNSCC) patients, the level and cellular origins of PD-L1-positive sEVs were assessed. (nature.com)
Numerous animal studies as well as observational and clinical trials have shown that atherosclerosis arises from immune activation, with several cell-type specific pathways involved. (bmrat.org)
Improved delivery of PLGA microparticles and microparticle-cell scaffolds in clinical needle gauges using modified viscosity formulations. (open.ac.uk)

Figure 2: Roles of extracellular vesicles in normal physiology and disease pathogenesis. (nature.com)
I cover mainly cell biology and physiology. (nottingham.ac.uk)

Figure 1: Biogenesis of extracellular vesicles and their interactions with recipient cells. (nature.com)
Biogenesis and classification of extracellular vesicles. (spandidos-publications.com)

Post-stroke neurological deficits increase the risk of cardiovascular diseases roughly by three times, and the ischemic brain transmits indirect cell death signals to the heart. (justia.com)

2013) focused on the role of cellular exocytic vesiculation in health, disease, and transfusion medicine, recognizing that microparticles (MPs), small membrane-derived vesicles which are derived from many cell types and released into the circulation under shear stress, complement activation, proapoptotic stimulation, cellular damage, or agonist interaction with cell surface receptors. (malvernpanalytical.com)

CMV targets vessel wall monocytes, macrophages, smooth muscle cells and endothelial cells leading to a multi-step process of endothelial dysfunction (14). (researchsquare.com)

Extracellular vesicles (EVs) are 10-500 nm membrane bound structures that play a crucial role in cellular communication especially in association to various diseases. (biomedcentral.com)

Although MSC EVs show great promise for regenerative medicine applications, insights into their uptake mechanisms by different target cells and the ability to control MSC EV properties for defined function in vivo have remained elusive knowledge gaps. (frontiersin.org)
The primary goal of this study is to elucidate how the basic properties of MSC derived EVs can be exploited for function-specific activity in regenerative medicine. (frontiersin.org)

Translational considerations in injectable cell-based therapeutics for neurological applications: concepts, progress and challenges. (open.ac.uk)

In the reticulocyte, as in most mammalian cells, portions of the plasma membrane are regularly internalized as endosomes, with 50 to 180% of the plasma membrane being recycled every hour. (wikipedia.org)
The cells secrete extracellular vesicles (EV) that may have an endosomal origin, or from evaginations of the plasma membrane. (frontiersin.org)
Extracellular vesicles (EVs) are a population of heterogeneous particles that originate from the endosomal system or plasma membrane. (bmrat.org)
Ionised lipids bind to negatively charged mRNA, supported by pegylated lipids, phospholipids, and cholesterol molecules to enable trafficking across the plasma membrane, delivering a payload of mRNA to cells for therapeutic effects. (trillium.de)

The interaction of platelets and vascular endothelial cells and the gene regulation of these types of cells under the stimulation of the anti-β2GPI/β2GPI complex remain unclear. (biomedcentral.com)
The miR-96 and -26a derived from platelets inhibits the migration and tube formation of human umbilical vein endothelial cells (HUVECs). (biomedcentral.com)

After attaching to target cells, EVs can change the physiological state of recipient cells through various pathways. (biomedcentral.com)
These include macrophage and smooth muscle cell (SMC) phenotypic switching and various inflammatory signaling, such as IL-33/suppression of tumorigenesis 2 (ST2), Ras-Raf-MEK-ERK pathways, and JAK-STAT signaling pathways 14 , 15 , 16 , 17 . (bmrat.org)
The pathogenesis is multifactorial and involves dysregulation in the signaling pathways, membrane lipids ratio disturbance, cell-cell adhesion disturbance, unfolded protein response, lysosomal and mitochondrial stress, autophagy dysregulation, and oxidative stress. (koreamed.org)

In 2012 Dr Norling attained a 5 year Arthritis Research UK Career Development Fellowship to investigate the protective actions of omega-3 fatty acid derived specialized pro-resolving lipid mediators (SPM) in inflammatory arthritis. (qmul.ac.uk)

Small and large extracellular vesicles derived from pleural mesothelioma cell lines offer biomarker potential. (otago.ac.nz)

Anatomy17

Organisms1

Diseases5

Chemicals and Drugs63

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes13

Disciplines and Occupations6

Technology, Industry, Agriculture19

Health Care2

Microvesicles9

Vesicle7

International Society for Extracellular Vesicles2

Exosomes30

Engineered extracellular vesicles1

Differentiation11

Analysis of extracellular2

Type of extracellular1

Flow Cytometry1

Exosome6

20172

Proteins8

Neutrophil-derived3

Stromal6

Intracellular4

Tissue17

Proliferation1

Microenvironment3

Platelet9

Mediators of intercellular communication1

Nanoparticle2

Endothelial cell10

Stem31

Vitro8

Epithelial2

Biomarkers1

Vesicles generated2

Biological5

Immunosuppressive1

Megakaryocytes2

Soluble1

Tumor8

Clinical3

Physiology2

Biogenesis2

Ischemic brain1

Membrane-derived1

Macrophages1

Structures1

Regenerative medicine2

Therapeutics1

Plasma membrane4

Platelets2

Pathways3

Inflammatory arthritis1

Small1