• It may also mediate the attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
  • Korpershoek JV, Rikkers M, Wallis FSA, Dijkstra K, Te Raa M, de Knijff P, Saris DBF , Vonk LA. Mitochondrial Transport from Mesenchymal Stromal Cells to Chondrocytes Increases DNA Content and Proteoglycan Deposition In Vitro in 3D Cultures. (mayo.edu)
  • 1: In the haematopoietic microenvironment, bone marrow stromal cells play an important role in regulating haematopoiesis by expressing various cytokines, including leukaemia inhibitory factor (LIF) and interleukin-6 (IL-6). (unboundmedicine.com)
  • However, the intracellular signal that regulates cytokine secretion in bone marrow stromal cells has not been determined. (unboundmedicine.com)
  • The aim of this study was to evaluate the role of mitogen-activated protein kinase (MAPK) family in serum-induced secretion of LIF and IL-6 by bone marrow stromal cells. (unboundmedicine.com)
  • 2: Transformed human bone marrow stromal cells (HS-5) were stimulated with foetal calf serum (FCS) to produce LIF and IL-6. (unboundmedicine.com)
  • We conclude that, in spite of their similar biological effects, they are differentially regulated at the level of MAPK activity in bone marrow stromal cells. (unboundmedicine.com)
  • TY - JOUR T1 - Role of mitogen-activated protein kinase family in serum-induced leukaemia inhibitory factor and interleukin-6 secretion by bone marrow stromal cells. (unboundmedicine.com)
  • The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. (origene.com)
  • Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins in human bone marrow stromal cells (hBMSC). (degruyter.com)
  • These microenvironments are classically defined as distinct cortex and medulla regions that each contain specialized subsets of stromal cells. (frontiersin.org)
  • Significantly, there are additional non-epithelial stromal cells (NES) that exist alongside TEC within thymic microenvironments, including multiple subsets of mesenchymal and endothelial cells. (frontiersin.org)
  • While adult lymphoid progenitor cells are produced by the bone marrow, their lineage commitment and development into mature T-cells is dependent on their migration into the thymus, where essential interactions with heterogeneous thymic stromal cells take place ( 1 ). (frontiersin.org)
  • Arguably, the most recognized and well-studied stromal populations in thymus biology are thymic epithelial cells (TEC) within the cortical and medullary areas, which are defined by their anatomical separation and function. (frontiersin.org)
  • While many studies have demonstrated the importance of cTEC and mTEC during T-cell development, thymic stromal microenvironments also contain heterogenous non-epithelial stromal (NES) populations in a similar manner to that seen in peripheral lymphoid tissues. (frontiersin.org)
  • Merckx G, Hosseinkhani B, Kuypers S, Deville S, Irobi J, Nelissen I, Michiels L, Lambrichts I, Bronckaers A. (2020) Angiogenic Effects of Human Dental Pulp and Bone Marrow-Derived Mesenchymal Stromal Cells and their Extracellular Vesicles. (uhasselt.be)
  • Bone marrow stromal cells (BMSC) are multipotent stem cells and are considered good candidates for cell restoration in injured brain tissue. (sld.cu)
  • Bone marrow stromal cells (BMSC) are adult stem cells with characteristics that make them an attractive candidate in cellular therapy for neurological diseases (1). (sld.cu)
  • In this work, for the first time, we adopt a multidisciplinary approach to characterize the morphological, biochemical, and molecular changes underlying the response of human bone marrow stromal cells to long-term simulated microgravity exposure during osteogenic differentiation. (lu.se)
  • The role for mechanical stimulation in the control of cell fate has been previously proposed, suggesting that there may be a role of mechanical conditioning in directing mesenchymal stromal cells (MSCs) towards specific lineage for tissue engineering applications. (um.edu.my)
  • Hu G, Liu P, Feng J, Jin Y. Transplantation with Bone Marrow Stromal Cells Promotes Wound Healing Under Chemotherapy through Altering Phenotypes. (ijbs.com)
  • Herein we characterized human fetal bone marrow stromal cells (hBMSCs) during wound healing in mice treated with cyclophosphamide (CTX). (ijbs.com)
  • There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
  • CD34 derives its name from the cluster of differentiation protocol that identifies cell surface antigens. (wikipedia.org)
  • In this study, we examined the potential of SF extract (SFE) to inhibit receptor activator of NF-κB ligand (RANKL) -induced osteoclast differentiation in cultured mouse-derived bone marrow macrophages (BMMs). (biomedcentral.com)
  • Pharmaceutical inhibition of osteoclast differentiation is a current therapeutic approach for the treatment of bone loss-related diseases. (biomedcentral.com)
  • Josefin Ekholm has been working for a long time to develop new protocols for differentiation of IPS cells into chondrocytes. (gu.se)
  • Members of the human serpin family regulate a diverse array of serine and cysteine proteinases associated with fibrinolysis, coagulation, inflammation, cell mobility, cellular differentiation, and apoptosis. (qedbio.com)
  • Our studies also demonstrated that the in vitro differentiation capacity of MDSC is restricted to cells at the peripheral sites. (purdue.edu)
  • Induction of p53 signaling elevated the differentiation of the bone marrow MDSC into granulocytic cell types. (purdue.edu)
  • From their computational model, the researchers at the LCSB identified a molecule called sFRP5 that keeps the neuronal stem cells inactive in old mice, and prevents proliferation by blocking the Wnt pathway crucial for cell differentiation. (sciencedaily.com)
  • Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
  • In order to eliminate the interference from the transplanted cells themselves, fetal stem cells were used for transplantation because the proliferation and differentiation potentials of fetal stem cells are superior to the adult ones ( 8 ). (ijbs.com)
  • Further, it regulates osteoblastic extracellular matrix such as osteopontin (OPN), osteocalcin (OCN), bone sialoprotein (BSP) in late differentiation. (frontiersin.org)
  • 2 (2007) observed that cell adhesion and proliferation, as well as the osteogenic differentiation of mouse mesenchymal stem cells (MSCs) to Ti discs were significantly similar to those on the plastic surface of the culture, indicating Ti as an excellent material for repairing hard tissue in the field of bone tissue engineering. (bvsalud.org)
  • The term "adhesion" to the biomaterial refers to the most important phase, since the quality of it will influence morphology and the capacity of cell proliferation and differentiation 3 . (bvsalud.org)
  • Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
  • Extracellular vesicle morphology was assessed using Transmission Electron Microscopy . (bvsalud.org)
  • Quantitative real-time PCR (qRT- PCR ) and western blotting analysis were performed to detect the expressions of miR-148a-5p, Smad4, transforming growth factor -ß1 (TGF-ß1), tissue inhibitor of metalloproteinase 1 ( TIMP-1 ), Collagen I, α- smooth muscle actin (α-SMA), and extracellular vesicle markers CD9, TSG101, CD63, and calnexin . (bvsalud.org)
  • Exosome (Exo) (30-200 nm) is a small single-membrane vesicle and exists in almost all mammalian cells [ 12 , 13 ]. (hindawi.com)
  • Beghein E, Devriese D, Van Hoey E, Gettemans J (2018) Cortactin and fascin-1 regulate extracellular vesicle release by controlling endosomal trafficking or invadopodia formation and function. (springer.com)
  • Here, we review recent progress in understanding extracellular vesicle biology and the role of extracellular vesicles in disease, discuss emerging therapeutic opportunities and consider the associated challenges. (nature.com)
  • FCS also induced activation of extracellular signal-regulated kinase (ERK), p38 MAPK and c-Jun NH(2)-terminal kinase (JNK). (unboundmedicine.com)
  • Phospholipase A(2) activation by poultry particulate matter is mediated through extracellular signal-regulated kinase in lung epithelial cells: regulation of interleukin-8 release. (cdc.gov)
  • Hence, it is hypothesized in this article that poultry PM induces the release of interleukin-8 (IL-8) by lung epithelial cells that is regulated upstream by the concerted action of cytosolic phospholipase A2 (cPLA2) and extracellular signal-regulated kinase (ERK). (cdc.gov)
  • Some studies have previously described the important role of extracellular vesicles (EVs) in the dissemination of a MDR phenotype. (up.pt)
  • Extracellular Vesicles from miR-148a-5p-Enriched Bone Marrow Mesenchymal Stem Cells Relieve Hepatic Fibrosis by Targeting Smad4. (bvsalud.org)
  • Bone marrow mesenchymal stem cells (BMSCs)-derived extracellular vesicles have been applied in many diseases . (bvsalud.org)
  • CD34 was first described on hematopoietic stem cells independently by Civin et al. (wikipedia.org)
  • Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. (wikipedia.org)
  • The presence of CD34 on non-hematopoietic cells in various tissues has been linked to progenitor and adult stem cell phenotypes. (wikipedia.org)
  • CD34 is expressed in roughly 20% of murine haematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
  • CD34+ is often used clinically to quantify the number of haemopoietic stem cells for use in haemopoietic stem cell transplantation. (wikipedia.org)
  • Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. (wikipedia.org)
  • i.e., they are multipotent hematopoietic stem cells. (wikipedia.org)
  • Injection of CD34+ hematopoietic stem cells has been clinically applied to treat various diseases including spinal cord injury, liver cirrhosis and peripheral vascular disease. (wikipedia.org)
  • Bone marrow-derived mesenchymal stem cells (MSC) have been promoted for multiple therapeutic applications. (nih.gov)
  • To improve the quality of liver grafts from extended-criteria donors donated after circulatory death (DCD), this study explored whether bone marrow mesenchymal stem cells (BMMSCs) combined with normothermic machine perfusion (NMP) have protective effects on DCD donor livers and the effects of ferroptosis in this procedure. (medscimonit.com)
  • Extracellular vesicles transfer chromatin-like structures that induce non-mutational dysfunction of p53 in bone marrow stem cells. (mpg.de)
  • Extracellular vesicles derived from bone marrow mesenchymal stem cells enhance myelin maintenance after cortical injury in aged rhesus monkeys. (bu.edu)
  • The exosomes (Exo) from human bone marrow mesenchymal stem cells (hBMSCs) after the transfection of miR mimics/inhibitor/si-PTEN were isolated and used to stimulate M4E and Hep2 cells. (hindawi.com)
  • Previous studies indicated that exosomal miR-29a-3p derived from human bone mesenchymal stem cells (hBMSCs) could efficiently suppress cell migrative ability and vasculogenic mimicry in glioma [ 15 ]. (hindawi.com)
  • Human bone marrow-derived mesenchymal stem cells (hBMSCs) were used to evaluate osteoconductivity and extracellular matrix secretion in vitro. (uib.no)
  • Besides stroke, my lab has also a main interest in using (dental) stem cells for tissue regeneration . (uhasselt.be)
  • Hereby we focus on the use of extracellular vesicles derived from dental pulp stem cells, a project with dr. (uhasselt.be)
  • Zong C, Bronckaers A, Vande Velde G, Willems G, Cadenas de Llano-Pérula M. (2022) In Vivo Micro-Computerized Tomography Tracking of Human Periodontal Ligament Stem Cells Labeled with Gold Nanocomplexes. (uhasselt.be)
  • In the future, it is hoped that stem cells can be used to treat osteoarthritis, the world's most common joint disease. (gu.se)
  • Within a large European research collaboration, work is underway to make it possible to use stem cells to rebuild cartilage tissue that has been worn out. (gu.se)
  • In AutoCRAT we develop new stem cell-based products, all of which have the potential to become a future treatment. (gu.se)
  • The first option being investigated in the EU project is mesenchymal stem cells, a type of adult stem cell taken from adipose tissue or bone marrow. (gu.se)
  • Another track is induced pluripotent stem cells, IPS, which is a method of inducing adult cells to become stem cells again. (gu.se)
  • Embryonic stem cell-derived microvesicles reprogram hematopoietic progenitors: evidence for horizontal transfer of mRNA and protein delivery. (nature.com)
  • Lai, R. C., Chen, T. S. & Lim, S. K. Mesenchymal stem cell exosome: a novel stem cell-based therapy for cardiovascular disease. (nature.com)
  • Human mesenchymal stem cell-conditioned medium improves cardiac function following myocardial infarction. (nature.com)
  • Stem Cell Res. (nature.com)
  • Reduction of myocardial infarct size by human mesenchymal stem cell conditioned medium. (nature.com)
  • Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers. (lu.se)
  • Objective: We developed a novel, streamlined protocol for generating chondrocytes from bone marrow-derived mesenchymal stem cells (BMSCs) in a 3D culture system that offers significant implications for the study of cartilage biology and the discovery of potential therapeutic interventions for cartilage-related and associated disorders. (lu.se)
  • Fibronectin (FN) is commonly used in the development of serum-free media for the expansion of mesenchymal stem cells (MSCs). (biomedcentral.com)
  • Mesenchymal stem cells (MSCs) are adult multipotent cells that were initially isolated from bone marrow[ 1 ] and characterized by the fibroblast-like appearance in culture and the capacities to form bone, adipose and cartilage. (biomedcentral.com)
  • The transplantation of bone marrow-derived mesenchymal stem cells (BMMSCs) alleviates neuropathology and improves cognitive deficits in animal models with Alzheimer's disease. (biomedcentral.com)
  • There is a signal crosstalk between autophagy and apoptosis, which may be regulated to produce synergistic effect on the preconditioning of stem cells. (biomedcentral.com)
  • Scientists have been able to rejuvenate stem cells in the brain of aging mice. (sciencedaily.com)
  • The revitalized stem cells improve the regeneration of injured or diseased areas in the brain of old mice. (sciencedaily.com)
  • The researchers expect that their approach will provide fresh impetus in regenerative medicine and facilitate the development of stem cell therapies. (sciencedaily.com)
  • Scientists from the Luxembourg Centre for Systems Biomedicine (LCSB) of the University of Luxembourg and from the German Cancer Research Center (DKFZ) have been able to rejuvenate stem cells in the brain of aging mice. (sciencedaily.com)
  • All cells making up our organs originate from stem cells. (sciencedaily.com)
  • With age, however, the stem cells of living organisms lose their ability to proliferate. (sciencedaily.com)
  • In order to create as accurate as possible computational models of stem cell behaviour, the LCSB's Computational Biology Group led by Prof. Antonio del Sol applied a novel approach. (sciencedaily.com)
  • Stem cells live in a niche where they constantly interact with other cells and extra-cellular components. (sciencedaily.com)
  • We stopped thinking about what external factors were affecting the stem cells, and started thinking about what the internal state of a stem cell would be like in its precisely defined niche. (sciencedaily.com)
  • The novel approach led to in a new computational model developed by Dr. Srikanth Ravichandran of the Computational Biology Group: "Our model can determine which proteins are responsible for the functional state of a given stem cell in its niche -- meaning whether it will divide or remain in a state of quiescence. (sciencedaily.com)
  • It was previously unknown why most of the stem cells in the brain of old mice remain in a state of quiescence. (sciencedaily.com)
  • Then the long-standing expertise in neural stem cells of the collaborators at the German Cancer Research Center (DKFZ) came in: Studying stem cells first in a dish and then later directly in mice, they could experimentally validate the computational prediction. (sciencedaily.com)
  • When neutralising the action of sFRP5, the quiescent stem cells did indeed start proliferating more actively. (sciencedaily.com)
  • With the deactivation of sFRP5, the cells undergo a kind of rejuvenation," del Sol says: "As a result, the ratio of active to dormant stem cells in the brain of old mice becomes almost as favourable as in young animals. (sciencedaily.com)
  • Our results constitute an important step towards the implementation of stem cell-based therapies, for instance for neurodegenerative diseases," Antonio del Sol says. (sciencedaily.com)
  • We were able to show that, with computational models, it is possible to identify the essential features that are characteristic of a specific state of stem cells. (sciencedaily.com)
  • It can also be used to model stem cells of other organs in the body. (sciencedaily.com)
  • Stem cell transplantation is a promising strategy for delayed wound healing caused by chemotherapy. (ijbs.com)
  • However, the fate of stem cells under chemotherapy has not been fully elucidated. (ijbs.com)
  • Nevertheless, myelosuppression can be reversed by autologous stem cell transplantation (SCT), even without additional toxicity ( 4 , 5 ). (ijbs.com)
  • Moreover, the state of stem cells is a prerequisite and basis for their application. (ijbs.com)
  • These may be attributed to the changes in the functional status of stem cells. (ijbs.com)
  • Therefore, fetal stem cells are suitable cells for clarifying the influences of CTX on cell functions. (ijbs.com)
  • In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
  • While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
  • More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
  • The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
  • Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
  • A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • Hematopoietic stem cell transplantation (HSCT) has been shown to be effective. (medscape.com)
  • In the prospective Autologous Stem Cell Transplantation International Scleroderma (ASTIS) trial, a phase 3 comparison of autologous HSCT with 12 successive monthly intravenous pulses of cyclophosphamide in 156 patients with early diffuse cutaneous systemic sclerosis, HCST was associated with higher treatment-related mortality than in the first year after treatment. (medscape.com)
  • mesenchymal stem cells, bone marrow, titanium. (bvsalud.org)
  • Paroxysmal nocturnal hemoglobinuria (PNH) was previously classified as purely an acquired hemolytic anemia due to a hematopoietic stem cell mutation defect. (medscape.com)
  • Early fate decisions in hematopoietic stem and progenitor cells. (lu.se)
  • HPSE is highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. (rndsystems.com)
  • Broadly separated into mesenchymal and endothelial cells, NES have been implicated in thymus organogenesis, thymocyte development, tolerance induction and development/maintenance of epithelial stroma. (frontiersin.org)
  • The mechanism of how the calcification signal from endothelial cells is transmitted to vascular smooth muscle cells (VSMCs) remains unknown. (spandidos-publications.com)
  • We demonstrate that α v β 3 integrin enhances BMDC recruitment and retention at angiogenic sites by mediating cellular adhesion and transmigration of BMDCs through the endothelial monolayer but not their release from the bone niche. (rupress.org)
  • These 2 members mediate leukocyte adhesions to endothelial cells but they also serve as receptors for iC3b (inactivated C3b). (medscape.com)
  • This disease is a defect in fucose metabolism (lack of fucosylation of the carbohydrate selectin ligands) that results in failure to express the ligand for E and P selectin, sialyl Lewis-X (CD15s) expressed on leukocytes and endothelial cells. (medscape.com)
  • Concurrent with these results, SeMet supplementation decreased lesion accumulation of M1 inflammatory type macrophages, and decreased the extent of extracellular trap release from phorbol myristate acetate (PMA)-stimulated mouse bone marrow-derived cells. (greenmedinfo.com)
  • The poultry PM-induced IL-8 release by the bone marrow-derived macrophages of cPLA2 knockout mice was significantly lower. (cdc.gov)
  • Her research work was focused on investigating the role of an extracellular matrix protein (fibronectin) on mediating cell-material interactions. (gla.ac.uk)
  • Glycosaminoglycans (GAGs) are essential functional components of the extracellular matrix (ECM). (degruyter.com)
  • Glycosaminoglycans (GAGs) are multifunctional polysaccharides of the extracellular matrix (ECM) responsible for ECM hydration and binding of cations and proteins due to their negative charge. (degruyter.com)
  • Under certain stimuli, the active enzyme is transferred back to the cell surface, where it participates in extracellular matrix degradation and remodeling. (rndsystems.com)
  • In addition, it increases cell adhesion to the extracellular matrix (ECM), independent of its enzymatic activity. (rndsystems.com)
  • This simple pellet-based 3D culture system allows for cell aggregation and spheroid formation, facilitating cell-cell and cell-extracellular matrix interactions that better mimic the in vivo cellular environment compared with 2D monolayer cultures. (lu.se)
  • The tumor microenvironment refers to the surrounding microenvironment in which tumor cells exist, including the surrounding blood vessels, immune cells, fibroblasts, bone marrow-derived inflammatory cells, various signaling molecules, and extracellular matrix. (techscience.com)
  • However, the addition of extracellular matrix in the media is not cost-effective and further investigations are needed to solve this problem. (biomedcentral.com)
  • In fact, MSCs can secrete various cytokines, growth factors and a series of extracellular matrix molecules including collagens and FN[ 22 - 26 ], which are the main substrates for MSC adhesion to the plastic. (biomedcentral.com)
  • Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. (antibodies-online.com)
  • Analyses include image observations of immunochemistry and immunofluorescence staining from extracellular matrix (ECM) production, and measuring related tenogenic and apoptosis gene marker expression. (um.edu.my)
  • OB release bone matrix rich in collagen type I and participate in the hydroxyapatite crystal formation in collagen fiber deposition process, namely bone mineralization process, eventually forming new bone. (frontiersin.org)
  • OC destroys the mineral and collagen matrix by secreting matrix metalloproteinases (MMPs), cathepsin K (CtsK), H + and Cl − , and eventually forming bone lacunae. (frontiersin.org)
  • This formation helps deposition and adhesion of the extracellular matrix on the bone-implant interface. (bvsalud.org)
  • Hydrogen peroxide was used to induce an oxidative stress model of hepatocyte IAR-20 cells to evaluate the protective effects of BMMSCs in vitro. (medscimonit.com)
  • MiR-29a-3p was enriched in hBMSC-derived Exo, and the Exo from miR-29a-3p mimics transfected hBMSCs could inhibit laryngocarcinoma cell malignant phenotypes in vitro and prevent tumor progression in vivo . (hindawi.com)
  • Towards a Mechanistic Model of Tau-Mediated Pathology in Tauopathies: What Can We Learn from Cell-Based In Vitro Assays? (ibecbarcelona.eu)
  • An oxygen-glucose deprivation model was employed to simulate in vitro SNI in PC12 cells. (techscience.com)
  • However, the quantity of MSCs is very low in bone marrow (about 0.001 to 0.01% of the mononuclear cells) and in vitro expansion is the prerequisite for their clinical application. (biomedcentral.com)
  • Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. (ijbs.com)
  • Many beneficial effects of MSCs are paracrine, dependent on extracellular vesicles (EVs). (nih.gov)
  • This study was aimed to observe if thrombin could stimulate FN secretion by human bone marrow MSCs and investigate the potential underlying mechanisms. (biomedcentral.com)
  • PCR analysis showed that human bone marrow MSCs expressed two subtypes of PARs, PAR-1 and PAR-2. (biomedcentral.com)
  • Although previous studies have reported that calcium signalling is involved in regulating many cellular processes in many cell types, its role in managing cellular responses to tensile loading (mechanotransduction) of MSCs has not been fully elucidated. (um.edu.my)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
  • Therefore, the present study aimed to evaluate the adhesion capacity of mouse bone marrow MSCs to smooth and plasmanitrided Ti surfaces in the cathodic cage configuration. (bvsalud.org)
  • Catalano M, O'Driscoll L (2020) Inhibiting extracellular vesicles formation and release: a review of EV inhibitors. (springer.com)
  • Abstract Non-small cell lung cancer (NSCLC) is a malignant tumor with high incidence worldwide. (techscience.com)
  • Title: Different ability of multidrug-resistant and-sensitive counterpart cells to release and capture extracellular vesicles Abstract: Cancer multidrug resistance (MDR) is one of the main challenges for cancer treatment efficacy. (up.pt)
  • Using knockin mice expressing functionally stunted β 3 integrin, we show that bone marrow transplantation rescues impaired angiogenesis in these mice by normalizing BMDC recruitment. (rupress.org)
  • Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. (degruyter.com)
  • Several investigations suggest that, besides their extracellular actions, also intracellular mechanisms of sGAG-derivatives seem possible. (degruyter.com)
  • Cancer cells have proven efficient in educating fibroblasts, within their microenvironment, to secrete EVs as communicative vessels for mediating phenotypic changes in recipient cells. (springer.com)
  • The miR-29a-3p level was decreased, and PTEN level was elevated in laryngocarcinoma tissues and the cancer cell lines. (hindawi.com)
  • RPL4 is overexpressed in NSCLC tissues and cells. (techscience.com)
  • CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. (wikipedia.org)
  • It is expressed on lymph node endothelia, whereas the L-selectin to which it binds is on the T cell. (wikipedia.org)
  • Villous atrophy and plasma cell infiltration of the jejunal mucosa are usually present and, sometimes, infiltration of the mesenteric lymph nodes. (msdmanuals.com)
  • Bone marrow-derived progenitor cell (BMPC) therapy has been shown to promote neovascularization, decrease infarct area and attenuate left ventricular (LV) dysfunction after MI. (indexindex.com)
  • Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. (antibodies-online.com)
  • Treatment for this condition remains difficult and largely ineffective, because the growth of cells in the wound is inhibited ( 2 ) and chemotherapy usually leads to myelosuppression, the latter of which further compromises the migration of multipotent cells from the bone marrow ( 3 ). (ijbs.com)
  • Recent studies have shown that extracellular vesicles (EVs) can be exploited by tumors to assist in cancer cell growth, proliferation, migration, invasion and metastasis. (springer.com)
  • Endoplasmic reticulum-Golgi intermediate compartment 3 (ERGIC3) promotes cell proliferation and metastasis in lung cancer, but its molecular mechanism is unclear. (techscience.com)
  • Overview of Plasma Cell Disorders Plasma cell disorders are a diverse group of disorders of unknown etiology characterized by Disproportionate proliferation of a single clone of B cells Presence of a structurally and electrophoretically. (msdmanuals.com)
  • In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
  • H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)
  • Proliferation-linked shifts in eligibility of T-cell progenitors, population dynamics, DN1 cells to undergo specification thus control kinetics of T-cell generation. (lu.se)
  • For the first time, this study demonstrated that the poultry PM-induced IL-8 secretion by human lung epithelial cells was regulated by cPLA2 activation through ERK-mediated serine phosphorylation, suggesting a mechanism of airway inflammation among poultry farm workers. (cdc.gov)
  • In conclusion, the knowledge gained in this study by elucidating the role of calcium in MSC mechanotransduction processes, and that in prolonged stretching results in non-apoptosis mediated cell death may be potential useful for regenerative medicine applications. (um.edu.my)
  • Most type II PNH cells (total lack of GPI-linked protein) are due to a frame shift mutation occurring in the early hematopoietic progenitor cells, resulting in same mutation in all blood cell lines. (medscape.com)
  • The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early haematopoietic and vascular-associated progenitor cells. (wikipedia.org)
  • The investigation of cell proteome highlighted how simulated microgravity affects a relatively low number of proteins compared to time and/or osteogenic factors and has allowed us to reconstruct a hypothetical pipeline for cell response to simulated microgravity. (lu.se)
  • This classification was abandoned because of the observation that surface proteins were missing not only in the RBC membrane but also in all blood cells, including the platelet and white cells. (medscape.com)
  • The common denominator in the disease, a biochemical defect, appears to be a genetic mutation leading to the inability to synthesize the glycosyl-phosphatidylinositol (GPI) anchor that binds these proteins to cell membranes. (medscape.com)
  • Due to its location on the X chromosome, and due to X inactivation in female somatic cells, only one mutation is required in either males or females to abolish the expression of GPI-linked proteins. (medscape.com)
  • In most plasma cell disorders, M-proteins (monoclonal immunoglobulin protein) are structurally similar to normal antibody molecules. (msdmanuals.com)
  • The aim of this study was to evaluate the osteoconductivity of 3D-printed PLATMC templates for bone tissue engineering, in comparison with the widely used 3D-printed polycaprolactone (PCL) templates. (uib.no)
  • 3D-printed PLATMC templates disclosed unique contact osteogenesis and significant higher amount of new bone regeneration, thus could be used to advantage in bone tissue engineering. (uib.no)
  • They divide and the resulting cells develop into specific tissue cells, forming the brain, lungs or bone marrow. (sciencedaily.com)
  • Bone is an active metabolism tissue, which constantly undergoes bone remodeling. (frontiersin.org)
  • Accordingly, the association of Ti implants with bone tissue culture may contribute to bone tissue regeneration 7 . (bvsalud.org)
  • Multiple Myeloma Multiple myeloma is a cancer of plasma cells that produce monoclonal immunoglobulin and invade and destroy adjacent bone tissue. (msdmanuals.com)
  • Aim: To evaluate the adhesion of mouse bone marrow mesenchymal cells (MBMMC) on different titanium surfaces. (bvsalud.org)
  • Tumor site monocytic-MDSC lacked the ability to differentiate into other cells types such as osteoclasts and granulocytic-MDSC, whereas the bone marrow and spleen counterparts could form both cell types. (purdue.edu)
  • The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. (antikoerper-online.de)
  • The patient was admitted with the bone marrow, microbiologic morphology, and PCR assay of tentative diagnosis of MTX-induced thrombocytopenia. (cdc.gov)
  • We showed that p38-MAPK pathway is involved in the regulation of MDSC-mediated immunoregulatory processes such that inhibition of p38 diminished MDSC expansion at the inflamed prostate, leading to greater numbers of T cells. (purdue.edu)
  • Further, there are signaling pathways that participate in regulation, such as Wnt channel, mitogen-activated protein kinase (MAPK) channel, bone morphogenetic protein (BMPs) channel, peroxisome proliferator activated-receptors (PPARs) channel, and Notch channel. (frontiersin.org)
  • In addition, mTEC screen thymocytes for their expression of high affinity αβ-TCRs, with negative selection limiting the release of these reactive T-cells into the peripheral pool ( 4 ). (frontiersin.org)
  • In addition to intrathymic selection, and during this medullary residency period, CD4 + and CD8 + single positive (CD4SP, CD8SP) thymocytes acquire the ability to proliferate in response to TCR stimulation, and undergo licensing for cytokine production, prior to exiting the thymus to join the peripheral T-cell pool as Recent Thymus Emigrants (RTE) ( 5 , 6 ). (frontiersin.org)
  • presence of young blood cells on the peripheral blood smears. (cdc.gov)
  • Specificity This antibody recognizes human SERPINB10 protein, a gene product that is expressed in human bone marrow cells. (qedbio.com)
  • Histoplasma capsulatum , a dimorphic fungus that caus- administered because disseminated histoplasmosis was es human disease, is endemic in North and Central highly suspected because of the bone marrow findings. (cdc.gov)
  • The human bone marrow harbors a CD45− CD11B+ cell progenitor permitting rapid microglia‐like cell derivative approaches. (lu.se)
  • To test this hypothesis, the widely used cultured human lung epithelial cells (A549) were chosen as the model system. (cdc.gov)
  • as a cell surface glycoprotein and functions as a cell-cell adhesion factor. (wikipedia.org)
  • Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. (wikipedia.org)
  • A key adhesion molecule expressed by these cells is the α v β 3 integrin. (rupress.org)
  • These properties determine the adhesion and behavior of cells in contact with the surface. (bvsalud.org)
  • CD11a/CD18 (LFA-1) expressed on lymphocytes is known to play an important role in lymphocyte trafficking (adhesion to vascular endothelium), as well as interactions to antigen presenting cells (APC). (medscape.com)
  • Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)
  • MiR-125b participates in the oncogenic role of lncRNA MALAT1 in the phenotypes including cell proliferative and invasive rates in laryngocarcinoma [ 6 ]. (hindawi.com)
  • Accepted: 24 October 2012 cell yield data, our models require that adult DN1 cells divide multiple times before becoming competent to progress into DN2 stage. (lu.se)
  • Using this vesicular delivery system, cancer cells can establish a new metastatic niche within distant sites, away from the primary tumor, thus favoring cancer progression. (springer.com)
  • Here, we show that although this receptor is present on most vascular and blood cells, the key regulatory function in tumor and wound angiogenesis is performed by β 3 integrin on bone marrow-derived cells (BMDCs) recruited to sites of neovascularization. (rupress.org)
  • MDSC that lack CAT2 have reduced capacity to suppress T cell responses both ex vivo and in vivo, as evidenced by increased T cell expansion in prostatitis and decreased tumor growth in cancer models. (purdue.edu)
  • MDR is a phenomenon by which tumor cells become resistant to several unrelated drugs. (up.pt)
  • Within the past decade, extracellular vesicles have emerged as important mediators of intercellular communication, being involved in the transmission of biological signals between cells in both prokaryotes and higher eukaryotes to regulate a diverse range of biological processes. (nature.com)
  • These form a complex network structure and precisely regulate bone formation ( 6 ). (frontiersin.org)
  • Extracellular vesicles from miR-148a-5p enriched BMSCs downregulated the mRNA and protein levels of TGF-ß1, TIMP-1 , Collagen I, and α-SMA. (bvsalud.org)
  • Extensive research on thymic epithelial cells (TEC) within the cortex and medulla has defined their essential roles during T-cell development. (frontiersin.org)
  • Cortical epithelial cells (cTEC) support the earliest thymocyte progenitor populations, guiding them through the cortex, directing them to a T-cell lineage fate and ensuring their functional qualities as self-MHC restricted cells through the process of positive selection ( 2 ). (frontiersin.org)
  • Medullary epithelial cells (mTEC), in conjunction with dendritic cells, then take over control of developing thymocytes. (frontiersin.org)
  • In this review, we cover how the non-epithelial compartment of thymic stroma represent essential cell populations in regulating thymus function. (frontiersin.org)
  • In addition, pathophysiological roles for extracellular vesicles are beginning to be recognized in diseases including cancer, infectious diseases and neurodegenerative disorders, highlighting potential novel targets for therapeutic intervention. (nature.com)
  • Figure 3: Therapeutic targeting and exploitation of extracellular vesicles. (nature.com)
  • Figure 2: Roles of extracellular vesicles in normal physiology and disease pathogenesis. (nature.com)
  • The present work aimed to: (i) compare the ability of drug-sensitive and their MDR counterpart cells to release and capture EVs and (ii) study and relate those differences with possible distinct fate of the endocytic pathway in these counterpart cells. (up.pt)
  • This difference in the release and capture of EVs may be associated with differences in the endocytic pathway between drug-sensitive and MDR cells. (up.pt)
  • Importantly, manipulation of the recycling pathway influenced the response of drug-sensitive cells to doxorubicin treatment. (up.pt)
  • Myeloid-derived suppressor cells (MDSC) are a heterogeneous population of immature cells that expand during benign and cancer-associated inflammation. (purdue.edu)
  • Osteoclast, derived from macrophage-mononuclear lineage, is a special myeloid cell. (frontiersin.org)
  • Angiogenesis is dependent on the coordinated action of numerous cell types. (rupress.org)
  • This was confirmed in vivo in the calvarial defect model, where PCL disclosed distant osteogenesis, while PLATMC disclosed greater areas of new bone and obvious contact osteogenesis on surface. (uib.no)
  • These vesicles are small spheres of biological information that separate from the surface of the cell. (gu.se)
  • Bone resorption occurs mainly on the bone surface, by the osteoclasts (OC) on bone metabolism in continuous absorption. (frontiersin.org)
  • Figure 1: Biogenesis of extracellular vesicles and their interactions with recipient cells. (nature.com)
  • Objectives: The objective of this study was to evaluate bone reconstruction using xenograft alone and associated with bone marrow aspirate concentrate (BMAC) and hyperbaric oxygen therapy. (mdpi.com)
  • In this study, we aimed to explore the specific mechanism of extracellular vesicles from BMSCs in liver fibrosis . (bvsalud.org)
  • Based on previous studies, this review will summarize the regulatory network mechanism of ferroptosis on bone remodeling and reveal the role of ferroptosis in osteoporosis (OP). (frontiersin.org)
  • The imbalance of bone formation and resorption is considered the main mechanism of osteoporosis. (frontiersin.org)
  • Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. (antibodies-online.com)