Low PU.1 expression in AML may account for low ATG gene expression in this disease. (hindawi.com)
This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. (sigmaaldrich.com)
Metastatic spread is responsible for the majority of cancer-associated deaths and is affected by a variety of factors, including the deregulation of gene expression, signal transduction, and epigenetic modification, as well as epithelial-to-mesenchymal transition (EMT) and the transformation of tumor cells into stem/progenitor cells. (jcancer.org)
Besides their canonical roles in translation, tRNAs also originate tRNA-derived small RNAs (tsRNAs), a class of small non-coding RNAs with regulatory functions ranging from translation regulation to gene expression control and cellular stress response. (mdpi.com)
A set of three expression constructs and a scrambled control is offered against every target gene with the guarantee that at least one of the three will have a knockdown effect of 70% or more on corresponding gene expression as determined by qRT-PCR, otherwise the constructs will be replaced one time free of charge. (genecopoeia.com)
He performed some of the first studies to identify transcription factors that regulate the gene expression and differentiation of eosinophils, which are known to play a major role in allergic and asthmatic responses. (ubc.ca)
thus, Pnn may affect crucial alternative splicing (AS) decisions and, in turn, impact cell-type specific gene expression. (molvis.org)
Well-regarded in the industry for high, reliable gene expression, SBI's lentiviral vectors also efficiently deliver RNAi. (systembio.com)
Transient gene expression in mammalian cells provides reasonable amounts of recombinant proteins and antibodies which is sufficient for most applications. (invivo.de)
Over the last two decades, studies based on genome-wide gene expression and functional profiling have revealed the great diversity of transcriptional alterations occurring in liver carcinogenesis. (aging-us.com)
Transcription factors (TFs) drive gene expression programs that shape specific phenotypes [ 3 ], and are frequently dysregulated in cancer [ 4 ]. (aging-us.com)
Correspondingly, most cancer signaling pathways seem to converge on one or more TFs, termed "master regulators" (MRs) [ 4 ], which direct tumor development, progression, and metastasis through hierarchical control of gene expression patterns. (aging-us.com)
Thus, MRs comprise typically a small number of TF-encoding genes (and their products) that control a disproportionate level of gene expression, giving rise to distinct molecular phenotypes associated with a particular disease. (aging-us.com)
Dormant breast cancer cells exhibit a distinct gene expression signature regardless of metastatic site. (biomedcentral.com)
In this study, we developed an efficient system for isolating rare dormant breast cancer cells in experimental metastases models and performed scRNA-seq comparing gene expression in dormant vs proliferative breast cancer cells. (biomedcentral.com)
Our studies suggest that the gene expression signature we identified in disseminated dormant breast cancer cells exists in the primary site and can be utilized to predict which patients are more likely to experience a breast cancer recurrence. (biomedcentral.com)
Systematic interrogation of gene function requires the ability to perturb gene expression in a robust and generalizable manner. (cdc.gov)
The signature of our top screening hits is significantly correlated with gene expression data from clinical melanoma samples. (cdc.gov)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
These dCas9-activator fusions targeted to the promoter region of endogenous genes can then modulate gene expression 7 - 11 . (cdc.gov)
Antibiotic1
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. (sigmaaldrich.com)
Lentiviral2
P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. (origene.com)
Using a deactivated or dead Cas9 nuclease fused to transcription activators, like Cas9-VPR, in combination with a guide RNA in either a synthetic or lentiviral format, the endogenous expression of a gene can be up-regulated by anywhere from 5 to 50,000+ fold! (horizondiscovery.com)
Promoter6
Promoter activity will vary by cell type and will affect dCas9-VPR expression, choosing an optimal promoter is important for robust gene overexpression. (horizondiscovery.com)
PB514B-1) drives expression of your gene-of-interest from the strong CMV promoter and expression of both an RFP reporter and puromycin resistance from the EF1α promoter. (reportergene.com)
Expression cassettes of all shRNA clones are fully sequenced including the promoter, sense and antisense target sequences, hairpin, terminator, and other linker sequences. (genecopoeia.com)
We further found that SET domain bifurcated 1 (Setdb1) was a SUMOylated protein and that SUMOylation promoted Setdb1 occupancy on the promoter locus of Pparg and Cebpa genes to suppress their expressions by H3K9me3. (deepdyve.com)
Our HIV-based pGreenPuro (EF1α) shRNA Cloning and Expression Lentivector shRNA Cloning and Expression Lentivector drives expression of your shRNA template from the H1 promoter, and after processing in the cell, your shRNA will be converted into siRNA. (systembio.com)
The vector also co-expresses puromycin and copGFP from the moderate EF1α promoter, with co-expression mediated by a T2A element. (systembio.com)
Genes9
We found a significantly lower expression of key autophagy- (ATG-) related genes in primary AML as compared to healthy granulocytes, an increased autophagic activity during all- trans retinoic acid- (ATRA-) induced neutrophil differentiation, and an impaired AML differentiation upon inhibition of ATG3, ATG4D, and ATG5. (hindawi.com)
The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
Validation studies - The OmicsLink™ expression ready ORF cDNA clones together with the same ORF clones that contain silent mutations in shRNA target sequence regions can be used for shRNA validation studies and gene/protein functional rescue studies for genes/proteins targeted by corresponding shRNA. (genecopoeia.com)
In adipocyte Senp2-deciency mice, accumulation of the SUMOylated Setdb1 suppressed the expression of Pparg and Cebpa genes as well as lipid metabolism-related target genes, which would decrease the ability of lipid storage in adipocytes. (deepdyve.com)
showed that knocking out Mage-D1 can induce the expression of autophagy-related genes by enhancing the activity and differentiation of osteoclasts, thereby accelerating the process of periodontitis 23 . (researchsquare.com)
Since the expression of genes defining discrete phenotypes is highly coordinated, application of reverse engineering algorithms to transcriptome datasets allows interpreting transcriptional networks by defining MRs and their associated regulons and gene circuits. (aging-us.com)
Housekeeping genes in the FSCN1 - ACTB (F-A) locus (Fig. 1a ) have relatively steady expression levels across various tissues and thus exogenous gene silencing resulting from chromatin inactivation might be avoided. (biomedcentral.com)
Expression of these genes had no impact on dormancy in murine models, but their expression correlated with disease-free survival in primary human breast cancer tumors, suggesting that these genes have predictive value in determining which patients are likely to recur. (biomedcentral.com)
The expression of dormancy-related genes identified in our analyses was consistently observed among different breast cancer dormancy models and correlated with breast cancer progression in patients. (biomedcentral.com)
Vector6
The PB-CMV-MCS-EF1α-RedPuro PiggyBac cDNA Cloning and Expression Vector (Cat. (reportergene.com)
The Super PiggyBac Transposase is delivered to the cell via the Super PiggyBac Transposase Expression Vector, which is co-transfected with one or more PiggyBac Vectors. (reportergene.com)
The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA. (reportergene.com)
Moreover, siRNA-mediated knockdown of KLF5 blocked the expression of CDX2, MUC2 and villin, but transfection of a KLF5 expression vector into esophageal epithelial cells promoted their transdifferentiation into columnar-like cells, as demonstrated by increased expression of the intestinal markers CDX2, MUC2 and villin. (jcancer.org)
To produce siRNAs for RNAi using the pSIF1-H1-H2Kk Cloning and Expression Lentivector, first clone your shRNA template into the unique BamHI or EcoRI sites in the vector. (systembio.com)
You can easily produce a recombinant lentivirus particle in HEK293T or HEK293FT cell line in high titer using GeneMedi's lentivirus Vector System, including the lentivirus expression plasmids (overexpression, shRNA or Crispr-cas9-gRNA), the envelope protein VSV-G expressing plasmid pMD2G and the packaging plasmid pSPAX2. (genemedi.net)
Markers2
CAFs and normal fibroblasts differ with respect to the expression of various markers, such as fibrotic markers, growth factors, chemokines and cytokines ( 9 ). (spandidos-publications.com)
Consistent and easy-to-use, SBI's PiggyBac Transposon System includes cloning and expression vectors that come with a range of markers as well as both constitutive and inducible promoters. (reportergene.com)
EGFP1
Enrichment for gene activation can now easily be done using the dCas9-VPR mRNA reagents that co-express either EGFP or puromycin with dCas9-VPR. (horizondiscovery.com)
Resistance2
Expression of Lcn2 is elevated by agents that promote insulin resistance and is reduced by thiazolidinediones. (diabetesjournals.org)
resistance is conferred by the expression of the pac gene. (fishersci.com)
ShRNA expression2
shRNA of varying lengths (19 to 29 bases) was designed using a proprietary algorithm to make shRNA expression constructs that have high knockdown efficiency with minimal off-target effect. (genecopoeia.com)
qRT-PCR - Targeting of the corresponding mRNA by a shRNA expression cassette leads to cleavage and degradation of the mRNA and possible subsequent reduction of protein expression. (genecopoeia.com)
Aminopeptidase2
Puromycin-sensitive amino peptidase also known as cytosol alanyl aminopeptidase or alanine aminopeptidase (AAP) (EC 3.4.11.14) is an enzyme that in humans is encoded by the NPEPPS gene. (wikipedia.org)
This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. (wikipedia.org)
Vectors3
Forward primer to sequence targets cloned in expression vectors. (origene.com)
Novel expression vectors compatible to the PiggyBac system - a well-established tool for transposon-mediated active gene transfer, were designed. (invivo.de)
However, it is difficult to capture the complexity of transcript isoform variance using these libraries, and large cDNA sequences are often difficult to clone into size-limited viral expression vectors. (cdc.gov)
Protein5
Functional assays by a reporter gene/protein - Alternatively, when the endogenous transcript level of gene of interest is low and/or when qRT-PCR and western blot analysis is not feasible or possible, KD effect can be measured by co-transduction of shRNA clone with an expression clone plasmid which is transcribed into a chimeric mRNA transcript consisting of a reporter gene and the target gene ORF. (genecopoeia.com)
The expression of Lcn2 is induced during 3T3-L1 adipogenesis in a CCAAT/enhancer-binding protein-dependent manner. (diabetesjournals.org)
C1QBP promoted the mRNA and protein expression of hypoxanthine catabolic enzyme XDH. (medsci.org)
This creates a pool of cells which all express the same protein or antibody, but with varying degrees of expression efficiency. (invivo.de)
Genetic Variants in ERAP1 and ERAP2 Associated With Immune-Mediated Diseases Influence Protein Expression and the Isoform Profile. (medlineplus.gov)
Proteins1
C1QBP significantly increased ROS level, apoptosis, and the expression of apoptotic proteins such as cleaved caspase-3 and bax/bcl2 via regulating XDH. (medsci.org)
Rats2
In conclusion, ENaC mRNA expression, especially αENaC, is increased in the very early phase of the experimental model of PAN-induced nephrotic syndrome in rats, but appears to escape from the regulation by aldosterone after day 3. (portlandpress.com)
There was a twofold increase in renal cortical alpha 1 (III) mRNA expression coincident with the onset of tubulointerstitial myofibroblasts infiltration in rats with PAN nephrosis beginning on day 6, which increased to a fivefold difference by day 10. (nih.gov)
Alix knockdown did not influence the number of EVs released by HLSCs, but it significantly decreased miRNA expression levels in the EVs and consequently their transfer to the endothelium. (spandidos-publications.com)
Fibroblasts3
The role of exosomes collected by ultracentrifugation were also analyzed as mediators of p53 expression in cancer cells and APJ expression in fibroblasts. (spandidos-publications.com)
APJ expression in fibroblasts co‑cultured with p53‑suppressed colon cancer cells (HCT116 sh p53 cells) was significantly lower than in control colon cancer cells (HCT116 sh control cells). (spandidos-publications.com)
MicroRNA 5703 enriched in exosomes derived from HCT116 sh p53 cells inhibited APJ expression, and inhibition of miR‑5703 diminished APJ suppression in fibroblasts caused by cancer cells. (spandidos-publications.com)
Cloning2
Generating siRNA from the pGreenPuro (EF1α) shRNA Cloning and Expression Lentivector. (systembio.com)
shRNA Cloning and Expression Lentivectors. (systembio.com)
MRNA expression4
Conversely, down-regulation of αENaC, βENaC and γENaC mRNA expression on day 3 occurred in the presence of high aldosterone concentrations, and was followed by a return of sodium excretion to control values. (portlandpress.com)
By combining in situ hybridization with immunohistochemistry, alpha 1(III) mRNA expression was colocalized to cells that labeled for alpha-smooth muscle actin identifying them as myofibroblasts. (nih.gov)
Interestingly, the major site of alpha 1(III) mRNA expression shifted to tubuloepithelial cells with the waning of myofibroblast infiltration on day 21. (nih.gov)
alpha 1(III) mRNA expression also localized to cells that labeled for alpha-smooth muscle actin. (nih.gov)
Moreover, the expression of C1QBP and XDH was lower in RCC tumors compared with the tumor-associated normal tissues, and their down-regulation was associated with higher Fuhrman grade. (medsci.org)
Receptor1
post-menopausal women with a higher serum FSH (≥78.3 IU/L) had higher serum TC and LDL-C levels than those with relatively lower FSH levels (40-78.3 IU/L), and ovariectomized mice had higher serum FSH and lipid levels and reduced hepatic LDL receptor expression. (nature.com)
Methods1
RESEARCH DESIGN AND METHODS- We examined Lcn2 expression in murine tissues and in 3T3-L1 adipocytes in the presence and absence of various stimuli. (diabetesjournals.org)
20231
2023) C/EBPβ Regulates TFAM Expression, Mitochondrial Function and Autophagy in Cellular Models of Parkinson's Disease. (systembio.com)
Autophagy1
Low expression of the autophagy initiator ULK1 in AML can partially be attributed to high expression of the ULK1-targeting microRNA-106a. (hindawi.com)
Progression1
We identify an important role for DDX5 in G 1 -S-phase progression where it directly regulates DNA replication factor expression by promoting the recruitment of RNA polymerase II to E2F-regulated gene promoters. (aacrjournals.org)
Apoptosis2
We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)
Apoptosis occurred 48 hours after EBF3 expression with caspase-3 activation. (aacrjournals.org)
Epithelial1
Bile acid treatment also increased the expression of KLF5, CDX2, MUC2 and villin in esophageal epithelial cells in a time-dependent manner. (jcancer.org)
Constructs1
First, we in order to generate the relevant constructs for stable expression in CHO cells. (invivo.de)
Activation1
Further analysis showed that higher expression of plakoglobin was able to promote activation of the PI3K/Akt/Bcl-2 pathway, which might protect the clusters from anoikis. (jcancer.org)