• Ectopic expression of dGATAe in the larval fat body, where it is normally absent, causes dramatic up-regulation of numerous innate immunity and gut genes, as judged by microarray analysis and in situ hybridization. (pnas.org)
  • To identify all classes of developmentally important genes, expression-based and other molecular screens are needed to supplement classical genetic screens. (pnas.org)
  • Conversely, the expression of both genes was suppressed in stomach epithelium cultivated with intestinal mesenchyme. (nii.ac.jp)
  • There is a hyperplastic response in the pigeon crop epithelium during 'lactation' that leads to localised cellular stress and expression of antioxidant protein-encoding genes. (biomedcentral.com)
  • The relative expression of the antimicrobial peptides encoding genes defensin ( defA , defB , defC ) and prolixicin ( prol ) was quantified by RT-qPCR. (biomedcentral.com)
  • In nub 1 mutants, which lack Nub-PD protein, excessive expression of antimicrobial peptide genes occurs in the absence of infection, leading to a significant reduction of the numbers of cultivatable gut commensal bacteria. (biomedcentral.com)
  • Signal transduction promotes nuclear translocation of the key NF-κB/Rel transcription factors Dorsal -related immunity factor (Dif) and Relish, which bind to a large number of target genes and activate their expression. (biomedcentral.com)
  • Constitutive expression of ROS and of subsets of AMP genes is evident in epithelial linings of the digestive, respiratory and reproductive organs [ 4 - 6 ]. (biomedcentral.com)
  • A system-wide understanding of the expression patterns of these genes would provide many new insights into their roles in normal and disease processes. (g3journal.org)
  • Here we used the protozoan Trypanosoma brucei as a model to investigate efficiency and specificity of RNAi generated by expression of long dsRNA of PFRA and PFRC genes, which code for flagellar proteins required for cell motility. (jove.com)
  • Reduction in the expression of any of these genes compromised the expression of the chaperone Hsp60 in response to mitochondrial unfolded protein stress without interfering with UPR er and caused perturbed mitochondrial morphology, consistent with persistent UPR mt . DVE-1 showed a nuclear localization and redistributed to nuclear puncta in response to conditions of mitochondrial unfolded protein stress. (sciencemag.org)
  • When CLPP-1 activity was decreased by RNAi, increased expression of the ubl-5 and hsp60 genes in response to conditions that cause mitochondrial unfolded protein stress was compromised, and the redistribution of DVE-1 to nuclear puncta was also blocked. (sciencemag.org)
  • The expression domains and regulation of labial and dve in the middle midgut were compared. (sdbonline.org)
  • The objective of this study was to compare the expression levels of AMPs (defensins and prolixicin) in the different midgut compartments and the fat body of R. prolixus infected with different T. cruzi strains. (biomedcentral.com)
  • Finally, another mutant simulation shows that cut expression might be shifted to late larval stages because of a potential interference with the early signaling process. (genetics.org)
  • A large degree of overlap is seen between expression patterns at these different stages of development, with the most common sites of expression being those involved in metabolic, endocrine and behavioral control. (g3journal.org)
  • Colored yeast fed to heterozygous larvae stains red throughout the length of the gut, but the same colored yeast accumulates only in the proventriculus in dve mutant larvae. (sdbonline.org)
  • To gain further insight into these interactions and processes, we have undertaken a whole-family analysis of nuclear receptor mRNA spatial expression patterns over the entire process of embryogenesis, as well as the 3rd instar wandering larva stage, by using high-resolution fluorescence in situ hybridization. (g3journal.org)
  • Among the more remarkable of the findings is that the majority of mRNA expression patterns observed show striking subcellular distributions, indicating potentially critical roles in the control of protein synthesis and subsequent subcellular distributions. (g3journal.org)
  • As with vertebrates, expression analysis has been limited to northern blotting or PCR-based analyses, and only on whole animals, thereby providing only a quantitative average of all spatial expression patterns. (g3journal.org)
  • RNAi experiments suggested that CLPP-1 acted upstream of UBL-5 (the expression of which is stimulated in response to conditions of mitochondrial unfolded protein stress) and of DVE-1 in the UPR mt . CLPP-1 was localized to the matrix of the mitochondria, where it appeared to be part of a large multisubunit complex. (sciencemag.org)
  • In this test, we discover that both CoCl 2 and an inducer of HO-1, hemin, could lead to the promotion of lateral root development, as well as the induction of HO-1 protein expression, HO activity, or LeHO - 1/2 transcripts, in lateral root initiation zone of tomato seedlings. (chemweb.com)
  • Therefore, this network serves to both restrict and induce cell type-specific expression. (phys.org)
  • Expression of wg at the dorsal-ventral (DV) boundary depends on two different signaling processes acting at different times during larval development and requiring a cross talk between the Notch (N) and Wg pathways ( Figure 1 ). (genetics.org)
  • Moreover, their expression was very low in the epithelium of newly formed proventricular glands. (nii.ac.jp)
  • This study found that the activities of two cis-regulatory enhancers are coordinated to drive dve expression in the fly eye. (sdbonline.org)
  • At late third instar (c), N activation and wg expression are maintained through an intercellular feedback circuit involving two layers of flanking cells on each side of the forming dorsal-ventral boundary. (genetics.org)
  • They specifically looked at how this network figured in Rhodopsin expression in several types of photoreceptors that are normally used for motion detection or color vision. (phys.org)
  • Specifically, in one FFL, Dve acts to repress Rhodopsin expression in the motion detecting photoreceptors. (phys.org)
  • Protease inhibitors also prevented the stimulation of ubl-5 expression in response to mitochondrial unfolded protein stress, suggesting that the protease activity of CLPP-1 is important for its function in the UPR mt . (sciencemag.org)
  • The expression of ap in dorsal cells induces fringe ( fng ) and Serrate ( Ser ) expressions in a cell-autonomous way. (genetics.org)
  • In the present study, we cloned and sequenced the goose TLR2-1, analyzed its expression in several tissues and verified its lipopeptide-mediated activation in HEK-293 T cells. (biomedcentral.com)
  • It was found that there are two endodermal expression domains: one is located immediately adjacent to the visceral mesoderm, and the other in a more interior inner endoderm layer. (sdbonline.org)
  • Negative autoregulation by Dve maintains expression from each enhancer at distinct homeostatic levels. (sdbonline.org)
  • In a P{lArB} enhancer detector collection, a line was found that showed upregulated expression within centrally to posteriorly located germarial cysts. (unibas.ch)
  • Tissue expression of geese TLR2-1 varied markedly, and was higher in kidney, cloacal bursa, skin and brain compared to other organs/tissues. (biomedcentral.com)