Expression of mammalian cloned in mammalian. Medical search. Frequent questions

ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with the C-terminal Myc-DDK tags. (origene.com)
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal mCFP (monomeric) tag. (origene.com)

Mammalian expression vector for tagging proteins with a C-terminal 3xFLAG epitope. (addgene.org)
ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart. (genscript.com)
The HPNV major capsid protein (VP1) sequence was cloned into the mammalian expression vector pCAGGS by using EcoRI and XhoI sites. (cdc.gov)
The modified S-trimer was produced by synthesizing and cloning the codon-optimized gene into a mammalian expression vector. (news-medical.net)
Novagen's pTriEx-1 vector contains bacterial, insect, and mammalian promoters. (the-scientist.com)
High-yield requirements might call for cloning of the target gene into an Escherichia coli expression vector, whereas subsequent activity studies might benefit from insect or mammalian cell expression. (the-scientist.com)
Until recently, testing of multiple systems was time consuming, involving cloning and verification of recombinants in each system-specific vector. (the-scientist.com)
The pTriEx-1 vector allows high-level gene expression in mammalian, E. coli , and insect cells. (the-scientist.com)
Serve as the entry vector in the PrecisionShuttle system to transfer the ORF sequence into any destination vectors for other tagging options or other expression platforms. (origene.com)
The Gateway recombination cloning system is designed for highly efficient transfer of your DNA into any Gateway expression vector from a single entry clone. (thermofisher.com)
The Center for Cancer Systems Biology of the Dana-Farber Institute created a collection of human open reading frames (ORFs) cloned into a Gateway-entry vector, known as the CCSB Human ORFeome Collection. (horizondiscovery.com)
These clones are derived from fully-sequenced Mammalian Gene Collection (MGC) full-length cDNAs and subsequently cloned into a Gateway recombinational entry vector. (horizondiscovery.com)
Transfection of mammalian cells with an expression vector containing either TIF3 or TEF-1d cDNA resulted in overexpression of the encoded protein. (cdc.gov)
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
The technology typically involves obtaining antibody genes from source cells including hybridomas and phage display libraries, amplifying and cloning the genes into an expression vector, introducing the vector into a host (bacteria, yeast, or mammalian cell lines), and achieving adequate expression of the functional antibody. (labmanager.com)
Simply email us the sequence of your gene and we'll synthesize and clone it into any of our inducible vectors for little more than the cost of the empty vector. (systembio.com)
PCR products were subsequently cloned into nmt promoter-driven expression vector pREP3x kindly provided by Dr. Susan Forsburg (Department of Biological Sciences at the University or college of Southern California Los Angeles CA) to obtain pREP3x-GIIα and pREP3x-GIIαVDEL. (exposed-skin-care.net)
With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)

These include bacterial system and three eukaryotic systems, such as yeast, insect and mammalian cells. (weizmann.ac.il)
These include cell-free extracts ( in vitro expression systems), and bacterial, yeast, insect, and mammalian cell systems, each with its own advantages and drawbacks. (thermofisher.com)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. (sigmaaldrich.com)
First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. (mdpi.com)
Bacterial artificial chromosome clones have been isolated from a trout library to further facilitate genomic characterization. (usda.gov)

The following Rtp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse development. (nih.gov)
High-efficiency full-length cDNA cloning. (nih.gov)
To investigate the physical structure of the chicken class I glycoprotein we have cloned the cDNA representing the BFIV locus of the B21 haplotype. (usda.gov)

With its end-to-end capabilities, Stelis handles development stages ranging from pre-clinical to commercial for recombinant proteins expressed in both microbial and mammalian systems. (pharmaceutical-business-review.com)
There have been three types of gap junctional proteins (connexins) identified in the mammalian lens: Cx43, Cx46, Cx50. (rosalindfranklin.edu)
One of the major aims of Dr. Ebihara's research project are to obtain a more detailed understanding of the biophysical properties of cloned lens gap junctional proteins and to localize the molecular determinants of permeability and gating of the gap junctional channels. (rosalindfranklin.edu)
Her expertise lies in protein biochemistry (including cloning, expression and characterization of proteins) and enzymology with a particular focus on structure, function and reactivity of mammalian peroxidases and their reaction products. (otago.ac.nz)

GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. (genscript.com)
One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence. (genscript.com)
Forward primer to sequence targets cloned in expression vectors. (origene.com)
V2_F Primer can be used to sequence the target sequence after cloning. (origene.com)
Dharmacon has not sequence verified each individual clone from these collections. (horizondiscovery.com)
Unlike traditional antibody production, they do not exhibit genetic drift, antibody expression variation, and antibody sequence mutation that can cause non-specific binding. (labmanager.com)
Here we statement that in the absence of GIIβ the catalytic subunit GIIα of the fission yeast WYE-687 (an organism displaying a glycoprotein folding quality control mechanism similar to that occurring in mammalian cells) folds to an active conformation able to hydrolyze does not display a consensus ER retention/retrieval sequence. (exposed-skin-care.net)

To investigate the expression of long interspersed element (LINE) 1, human endogenous retrovirus (HERV) K10, and short interspersed element-VNTR-Alu element (SVA) retrotransposons in ejaculated human spermatozoa by means of reverse-transcription (RT) polymerase chain reaction (PCR) analysis as well as the potential incorporation of cloned human and mouse active retroelements in human sperm cell genome. (nih.gov)
Retroelement expression in human spermatozoa, incorporation of cloned human and mouse active retroelements in human sperm genome, and de novo retrotransposition events in human spermatozoa. (nih.gov)
A genome-scale library of Gateway-adapted human ORF clones, the CCSB Human ORFeome Library (aka hORFeome V5.1) is ideal for transferring thousands of open reading frames into a variety of protein expression systems. (horizondiscovery.com)
Among the factors thought to contribute to the greater success in cloning cattle are the relatively late embryonic genome activation speciﬁc for this species [16 -18] and the optimization of reproductive technologies, such as in vitro embryo production and embryo transfer, brought about by the cattle industry [19]. (sibi.org)
To facilitate mammalian genome engineering applications, we provide a web-based software tool to guide the selection and validation of target sequences as well as off-target analyses. (cdc.gov)

For T7-regulated E. coli expression systems, MagicMedia Expression Medium can increase protein yields up to 10-fold. (thermofisher.com)
High efficiency chemically competent E. coli cells suitable for T7 expression with enhanced reduction of basal expression. (neb.com)
Enhanced BL21 derivative, chemically competent E. coli cells suitable for high efficiency transformation and protein expression. (neb.com)
A T7 expression plasmid containing a gene encoding an E. coli protein was transformed into each host, grown to 0.6 OD and induced for 3 hours. (neb.com)

The molecular mechanisms involved in the specific regulation of PDGF alpha R expression are unknown, but transcriptional regulation of the PDGF alpha R gene is most likely to be involved. (nih.gov)
This study describes the molecular cloning of the non-coding exon 1 and approximately 2 kb of 5' flanking region of the human PDGF alpha R gene. (nih.gov)
Up-regulation of expression of translation factors - a novel molecular mechanism for cadmium carcinogenesis. (cdc.gov)
The molecular mechanisms potentially responsible for cadmium carcinogenesis were investigated by differential gene expression analysis of Balb/c-3T3 cells morphologically transformed with cadmium chloride. (cdc.gov)
These findings demonstrate that the up-regulation of expression of TIF3 and TEF-1d is a novel molecular mechanism responsible, at least in part, for cadmium carcinogenesis. (cdc.gov)
Molecular cloning of a novel mammalian calcium-dependent protease distinct from both m- and mu-types. (wikidoc.org)

Target genes of interest cloned in the appropriate expression vectors are introduced into host cells. (weizmann.ac.il)
Gateway technology allows highly efficient transfer of the gene of interest into any of a number of expression vectors from a single entry clone. (thermofisher.com)
The extremely wide variety of Gateway cloning-compatible expression vectors available makes the Gateway system of recombination cloning ideal for protein expression studies. (thermofisher.com)
Get inducible expression vectors that offer titratable expression with low background. (systembio.com)

Dharmacon is a distributor of multiple gene expression clone collections (cDNAs and ORFs). (horizondiscovery.com)

The mechanisms underlying the appearance of asymmetry between cells in the early embryo and consequently the specification of distinct cell lineages during mammalian development remain elusive. (nature.com)
This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg . (invivogen.com)
Expression of SSEA-4 is down-regulated following differentiation of human EC, ES, and iPS cells. (stemcell.com)
In contrast, the differentiation of mouse EC, ES, or iPS cells may be accompanied by an increase in SSEA-4 expression. (stemcell.com)
His work has led to major advances in our understanding of mammalian cloning and embryonic stem cells. (wraltechwire.com)
In melanocytic cells CAPN3 gene expression may be regulated by MITF . (wikidoc.org)
SSCs are adult-tissue stem cells in the mammalian testis that balance self renewing and differentiating fate decisions to give rise to and sustain the entire spermatogenic lineage. (utsa.edu)
Comparison of soluble extracts from uninduced (-) and induced (+) cells shows superior control of basal expression in the T7 Express hosts while maintaining high levels of induced expression. (neb.com)
Nevertheless whereas constitutes an ideal organism to study the role of GIIβ because it has a glycoprotein folding quality control mechanism similar to that occurring in mammalian cells and it expresses an active GT (Fernández DH5α and JA226 were utilized for cloning purposes whereas recombinant protein expression was carried out using BL26 cells. (exposed-skin-care.net)
Mammalian cells produce PGs, which are involved in biological processes such as cell growth and differentiation. (lu.se)
Both the structure of the PGs and the GAG chains, and the expression of the enzymes involved in their biosynthesis and degradation, vary between normal cells and tumor cells. (lu.se)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
The sphingomyelin that accumulates in the lysosomes of Niemann-Pick disease-affected cells is thought to arise from the degradation of the cells and their organelles because it is a major component of all mammalian cell membranes. (medscape.com)

RTP family members induce functional expression of mammalian odorant receptors. (genscript.com)
This 5' flanking region is a functional promoter of the PDGF alpha R gene as concluded from its capacity to drive luciferase reporter gene expression in an orientation dependent way. (nih.gov)
Monoclonal antibodies to the FLAG epitope served to monitor cell surface expression for functional analysis of the BFIV21 class I glycoprotein. (usda.gov)

The development of cystic tissue requires the activation of transforming growth factor-ß (TGFß) signaling, which promotes the expression of a procystic, fibronectin-rich extracellular matrix and which itself is perceived by a changing profile of integrin receptors on the cystic epithelium. (bvsalud.org)

Protein expression is influenced by many factors that may vary between experiments or laboratories. (genscript.com)
Expression is screened on a small scale in 4-10 ml cultures and soluble and insoluble cellular fractions are analyzed by SDS-PAGE for protein expression. (weizmann.ac.il)
There are many options available for protein expression from cloned DNA. (thermofisher.com)
The variety of expression choices available makes the Gateway system of recombination cloning ideal for protein expression studies. (thermofisher.com)

The MISSION ® pLKO.1-puro Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION ® shRNA library clones. (sigmaaldrich.com)
This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. (sigmaaldrich.com)
When conducting experiments using MISSION ® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. (sigmaaldrich.com)

pFUSE-CHIg-rG is a cloning plasmid that expresses the constant region of the rabbit IgG heavy chain . (invivogen.com)
A T7 expression plasmid and the same plasmid containing a gene encoding a toxic mammalian protein were transformed into each host. (neb.com)

For improving our understanding of epigenetic regulation of gene expression: the biological mechanisms that affect how genetic information is variably expressed. (wraltechwire.com)
Their failures or incompletion (regulation and gene expression, epigenetic, etc.) are attributed to the inviability and losses detected in the laboratory as well as the pathologies observed during the animal´s pregnancy and offspring after birth or later. (sibi.org)
We sought to identify members of the tumor necrosis factor superfamily (TNFSF) because of their important roles in the regulation of mammalian B cell survival and antibody production. (usda.gov)
They have been hypothesized to have wide-ranging functions in the mammalian central nervous system, including pain modulation, opiate function, cardiovascular regulation, and neuroendocrine function. (aspetjournals.org)

It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain (CH) variable region. (invivogen.com)
For mammalian cell culture manufacturing, Stelis adopts the latest single-use technology of Merck-Millipore for both upstream and downstream operations. (pharmaceutical-business-review.com)

To increase the probability that a given protein will be expressed in soluble, active form, and in high yield several expression systems are used. (weizmann.ac.il)

In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. (cellsignal.com)

It eliminates the need for the tedious mRNA purification process required for conventional cloning techniques. (sigmaaldrich.com)
We have cloned the NPFF gene from human, bovine, rat, and mouse, and show that the precursor mRNA encodes for all three of the biochemically identified peptides (NPFF, NPAF, and NPSF). (aspetjournals.org)
We demonstrate that NPFF precursor mRNA expression by Northern analysis and map sites of expression by in situ hybridization. (aspetjournals.org)
Aarnisalo AA, Nieminen M, Kontinen V, Vilim FS, Kalso E, Ziff E and Panula P (1997) Expression of NPFF mRNA in carrageenan inflammation in the spinal cord. (aspetjournals.org)

Dr Paumann-Page's main research interest is how the reactions of mammalian peroxidases are involved in health and disease. (otago.ac.nz)

Dr Paumann-Page is investigating well-characterized cell lines from the New Zealand melanoma cell panel to characterize peroxidasin expression levels, activity, cellular location and the effect of modulation of peroxidasin protein levels and activity on cell invasiveness. (otago.ac.nz)

Such clones are the best for detection and purification of the transgene using anti-Myc or anti-DDK antibodies . (origene.com)
While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)

Figure 2: The self-organization theory of early mammalian development. (nature.com)
Expression of the platelet-derived growth factor alpha receptor (PDGF alpha R) is strictly regulated during mammalian development and tumorigenesis. (nih.gov)
The cell line and process development in microbial and mammalian systems is supported by extensive physicochemical and bio-analytical characterisation capabilities. (pharmaceutical-business-review.com)
2005). Finally, negative revealed that SSEA4 is detectable in the early neuroepi- selection strategies have been also developed as an alter- thelium, and its expression decreases as development native method to enrich for NSCs from both adult proceeds. (lu.se)

We are testing the hypothesis that specific transcription factors form regulatory networks to execute gene expression programs important for SSC fate decisions (self-renewal and differentiation), and ultimately, spermatogenesis. (utsa.edu)
Antisense transcription in the mammalian transcriptome. (nih.gov)

The constructs were electroporated into and expression levels were regulated with thiamine added to the media. (exposed-skin-care.net)

Our findings demonstrate that a single intramuscular injection in mice of AAV encoding R1a-B6 fused to Fc fragments of different isotypes equipped either, with or without antibody dependent cellular cytotoxicity (ADCC) activity, was able to drive sustained high-level expression (0.5-1.1 mg/mL) in sera with no evidence of reduction for up to 6 months. (frontiersin.org)

Obtaining the perfect balance between yield and activity may also inspire testing of several different expression systems. (the-scientist.com)
Requirements for protein solubility, functionality, and yield are often the most important factors to consider when choosing an expression system, but the time and labor required for subcloning and optimizing expression can also have a significant impact. (thermofisher.com)

True cloning performed by nuclear transfer from an adult and differentiated somatic cell to a previously enucleated egg (somatic cell nuclear transfer, SCNT), gives rise to a new cell, the nuclovulo (nucleus+ovum), distinct from the zygote because the sperm is not involved in its creation, while both can develop as embryos and give rise to offspring. (sibi.org)

Our results confirm the evolutionary conservation of NPFF, NPAF, and NPSF neuropeptide expression in mammalian brain. (aspetjournals.org)

Stelis BioSource has invested in high-performance technologies that support robust and efficient processes for both microbial and mammalian systems. (pharmaceutical-business-review.com)
Stelis BioSource's state-of-art, 200,000 ft 2 , commercial-scale bio-manufacturing facility in Bangalore has the capability to produce simple and complex recombinant biotherapeutics from both microbial and mammalian cell culture systems. (pharmaceutical-business-review.com)
Get inducible gene expression for mammalian systems with SBI. (systembio.com)

These findings can be integrated in a new unified framework that regards the early mammalian embryo as a self-organizing system. (nature.com)
Using the best expression system for your protein and application is key to your success. (thermofisher.com)

The full length cDNAs for TIF3 and TEF-1d were cloned and sequenced. (cdc.gov)

We tested various specimens (blood, cerebrospinal fluid, intraocular fluid, serum, and tissues) from the organ donor and recipients by serology, RT-PCR, immunohistochemistry, metagenomic next-generation sequencing, and host gene expression, and conducted a traceback of blood transfusions received by the organ donor. (cdc.gov)

To determine the structural requirements for beta 4GalT7 activity, we have cloned and expressed the enzyme and designed a focused library of 2-naphthyl beta-D-xylopyranosides with modifications of the xylose moiety. (lu.se)

We performed northern blot analyses for the guide RNA truncations and found increased levels of expression for the longer tracrRNA sequences, suggesting that improved target cleavage was at least partially due to higher sgRNA expression or stability ( Fig. 1c ). (cdc.gov)

Comparison of the relative transformation efficiencies demonstrates that the T7 Express hosts provide the levels of control necessary for transformation of potentially toxic clones. (neb.com)
BL21(DE3) could not be transformed with the toxic clone. (neb.com)

Anatomy35

Organisms33

Chemicals and Drugs66

Analytical, Diagnostic and Therapeutic Techniques and Equipment33

Phenomena and Processes90

Disciplines and Occupations1

Information Science4

Health Care2

Expressed in mammalian cells2

Vector19

Bacterial6

CDNA4

Proteins4

Sequence7

Genome5

Coli4

Molecular6

Vectors4

ORFs1

Cells14

Functional3

Receptors1

Protein expression4

ShRNA3

Plasmid2

Regulation4

Upstream2

Soluble1

Genetic1

MRNA4

Reactions1

Cell lines1

Antibodies2

Development4

Transcription2

Constructs1

Mice1

Yield2

Distinct1

Brain1

Systems3

System2

Full-length1

Host1

Structural1

Target1

Toxic2