• TNF-α induced Hif-1α/VEGF/VEGFR-2/ERK signaling pathway, while propofol could inhibit it. (researchsquare.com)
  • Conclusion: TNF-α could decrease the expression of occludin via Hif-1α/VEGF/VEGFR-2/ERK signaling pathway, which was attenuated by propofol. (researchsquare.com)
  • Moreover, both peptides also reduce the activation of p38 mitogen-activated protein kinase (MAPK), a pro-apoptotic signaling pathway, which is activated by VEGFR-1 and 2 receptors. (uniroma5.it)
  • This effect appeared to be achieved via inhibition of the PERK/ATF4/CHOP signaling pathway and suppression of VEGF expression levels. (spandidos-publications.com)
  • Translational suppression of HIF-1α by miconazole through the mTOR signaling pathway. (lktlabs.com)
  • The stimulation of the VEGF secretion by FN confirms the important role of FN in angiogenesis which is also described in the literature. (uni-marburg.de)
  • The mineral dust-induced gene, mdig, regulates angiogenesis and lymphangiogenesis in lung adenocarcinoma by modulating the expression of VEGF-A/C/D via EGFR and HIF-1α signaling. (nih.gov)
  • Even though the exact mechanisms underlying ischemic injury in the muscle are not completely understood, hypoxia-inducible factor-1 alpha (HIF-1α) has emerged as an attractive target to enhance post ischemic angiogenesis. (frontiersin.org)
  • Hypoxia-inducible factor 1 (HIF-1) participates in tumor angiogenesis by upregulating target genes, such as vascular endothelial growth factor (VEGF). (oncotarget.com)
  • Over-expression of hypoxia-inducible factor in multiple myeloma plasma cells strongly induced in vivo angiogenesis, and administration of a CXCL12 antagonist decreased hypoxia-inducible factor-induced angiogenesis. (haematologica.org)
  • Conclusions Hypoxia-inducible factor-2 is a newly identified regulator of CXCL12 expression in multiple myeloma plasma cells and a major contributor to multiple myeloma plasma cell-induced angiogenesis. (haematologica.org)
  • 14 , 15 HIF-1 and HIF-2 mediate adaptive responses to hypoxia by inducing the transcription of genes associated with erythropoiesis, glycolytic metabolism, cell survival and angiogenesis. (haematologica.org)
  • In such case, HIF-1 promotes angiogenesis and other typical cellular responses for hypoxia. (czytelniamedyczna.pl)
  • The effectiveness of the strategy was validated by showing that transgenic expression of VEGF164 in Col2-Cre;VEGF(f/f) mice stimulated angiogenesis in the perichondrium, fully corrected the excessive hypoxia of VEGF-deficient chondrocytes, and completely prevented chondrocyte death. (ox.ac.uk)
  • Transgenic VEGF164 induced massive angiogenesis in the perichondrium, yet this only partially relieved the aberrant hypoxia present in HIF-1α-deficient cartilage and thereby likely inflicted only a partial rescue effect. (ox.ac.uk)
  • Hence, regulation of the metabolic pathways by HIF-1α and VEGF-dependent regulation of angiogenesis coordinately act to maintain physiological cartilage oxygenation. (ox.ac.uk)
  • Inhibition of the type I TGF-β receptor with SB505124 does not alter endothelial activation in vitro , but decreases pro-angiogenic gene expression and suppresses angiogenesis in vivo . (oncotarget.com)
  • Expression of miR-217 and HIF-1α/VEGF pathway in sufferers with diabetic foot ulcer and its impact on angiogenesis of diabetic foot ulcer rats. (tech4seo.com)
  • Zhu Y, Wang Y, Jia Y, Xu J, Chai Y. Roxadustat promotes angiogenesis by way of HIF-1α/VEGF/VEGFR2 signaling and accelerates cutaneous wound therapeutic in diabetic rats. (tech4seo.com)
  • CLL demonstrates enhanced angiogenesis, with increased MVD, upregulated VEGF and downregulated TSP-1. (biomedcentral.com)
  • A number of molecules, including vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and hypoxia-inducible factor 1α (HIF-1α) have been identified as positive regulators of angiogenesis. (biomedcentral.com)
  • These are kept in balance by negative regulators of angiogenesis including thrombospondin-1 (TSP-1) [ 2 ] and interferon β (IFNβ) [ 3 ]. (biomedcentral.com)
  • The microenvironmental changes stimulate the expression of hypoxia-inducible factors (HIFs), which promote the choroidal endothelial cell transmigration across the retinal pigmented epithelium (RPE) into neurosensory retina, where they proliferate into new vessels under stimulation of the vascular endothelial growth factor (VEGF). (uniroma5.it)
  • Accordingly, it was demonstrated that protein expression of collagen type IV in human vascular smooth muscle cells is regulated by both SMAD3 protein and TGFβ mediated stimulation of mRNA. (wikipedia.org)
  • Vascular endothelial growth factor (VE6E), which is under the control of hypoxia inducible factor-1 alpha (HIF-1 alpha), stimulates the proliferation of endothelial cells and increases cell permeability, promoting the growth, spread and metastasis of tumors. (fapesp.br)
  • Furthermore, it was revealed that treatment with vitexin induced hypoxia inducible factor 1α subunit (HIF‑1α) and vascular endothelial growth factor (VEGF) protein expression, and suppressed phosphorylated‑p38 MAP kinase (p38) protein expression in sevoflurane‑induced newborn rat. (spandidos-publications.com)
  • Hypoxia inducible factor-1 (HIF-1) is an oxygen-sensitive transcriptional regulator that regulates more than 70 downstream target genes, including vascular endothelial growth factor, erythropoietin, glucose transporter and so on ( 5 ). (spandidos-publications.com)
  • VEGF is purified from bovine pituitary follicular cells in 1959 and is a vascular endothelial cell-specific mitogen that promotes the growth of vascular endothelial cells. (spandidos-publications.com)
  • The expression of vascular endothelial growth factor, vascular endothelial growth factor-2 and hypoxia-inducible factor-1 were detected by Western blot. (ijpsonline.com)
  • The purpose of this study was to identify potential therapeutic strategies to slow down or prevent the expression of early-onset epithelial to mesenchymal transition (EMT) marker proteins (fibronectin and alpha smooth muscle actin, α-SMA) without sacrificing the synthesis and accumulation of the prosurvival protein vascular endothelial growth factor (VEGF) in cultured virally transformed human lens epithelial (HLE) cells. (molvis.org)
  • 9. Chan CK, Vanhoutte PM. Hypoxia, vascular smooth muscles and endothelium Acta Pharmaceutica Sinica B. 2013;3(1):1-7. (scielo.br)
  • The present study investigated the effects of GSK2606414 on proliferation, apoptosis, and the expression of activating transcription factor 4 (ATF4), CCAAT/enhancer‑binding protein homologous protein (CHOP) and vascular endothelial growth factor (VEGF) in human retinal pigment epithelial (RPE) cells under endoplasmic reticulum (ER) stress. (spandidos-publications.com)
  • In addition, RPE cells proliferate and secrete various proangiogenic factors, including vascular endothelial growth factor (VEGF), which serves an important role in AMD-associated CNV ( 4 ). (spandidos-publications.com)
  • Glucocorticoids bind steroid receptors in the cytoplasm, alter DNA expression and inhibit formation of inflammatory mediators such as interleukin-6 (IL-6), IL-8, prostaglandins, and vascular endothelial growth factor (VEGF). (dovepress.com)
  • Hypoxia-inducible factor-1α (HIF-1α) is a key transcription factor to initiate the expressions of distinct pro-angiogenic growth genes, particularly the expression of vascular endothelial growth factor (VEGF). (elis.sk)
  • The two most important molecular targets for new drugs in renal cancer are vascular endothelial growth factor (VEGF) and mammalian target of rapamycin (mTOR) pathways. (czytelniamedyczna.pl)
  • To investigate the role of vascular endothelial growth factor (VEGF) at different phases of diabetic retinopathy (DR), we assessed the retinal protein expression of VEGF-A(164) (corresponding to the VEGF(165) isoform present in humans, which is the predominant member implicated in vascular hyperpermeability and proliferation), HIF-1 alpha and PKC beta/HuR pathway in Ins2 ( Akita ) (diabetic) mice at different ages. (unisr.it)
  • Here, we discuss current understanding of the process of vascular calcification, focusing specifically on the discrete and synergistic effects of calcium and phosphate in mediating vascular smooth muscle cell apoptosis, osteochondrocytic differentiation, vesicle release, calcification inhibitor expression, senescence, and death. (scite.ai)
  • Vascular Endothelial Growth Factor-A (VEGF-A) is transcriptionally regulated by oxygen tension variations, via Hypoxia Inducible Factor-1 α (HIF-1α) [ 2 ]. (biomedcentral.com)
  • a Vascular endothelial growth factor-A (VEGF-A) mRNA levels in Normal (NF) and Fibrotic (FF) fibroblast total RNA lysates in response to 24 h exposure to hypoxia and hyperoxia. (biomedcentral.com)
  • Previously we found that estrogen aided in this neovasculogenesis via recruitment of bone marrow derived endothelial progenitor cells (BM-EPCs), leading to increased vessel formation and vascular endothelial growth factor (VEGF) production in vivo . (biomedcentral.com)
  • A similar phenotype was observed in mice with genetic ablation of either all or specifically the diffusible isoforms of vascular endothelial growth factor (VEGF), a prime angiogenic target of HIF-1α. (ox.ac.uk)
  • The results demonstrated that Vitexin pretreatment significantly reduced neuronal apoptosis, and inhibited caspase‑3 activity, apoptosis regulator BAX protein expression and malondialdehyde levels in sevoflurane‑induced newborn rats. (spandidos-publications.com)
  • Pretreatment of murine myoblast (C2C12) cells with octyl-D-carnosine or carnosine enhanced HIF-1α protein expression, VEGF mRNA levels and VEGF release under hypoxic conditions. (frontiersin.org)
  • Results Strong hypoxia-inducible factor-2 protein expression was detected in CD138 + multiple myeloma plasma cells in patients' biopsy specimens. (haematologica.org)
  • The mRNA and protein expression level of HIF-1α and VEGF were remarkably up-regulated in McA RH-7777 cells under hypoxic conditions, respectively. (elis.sk)
  • However, diabetic Ins2 ( Akita ) mice recapitulate the same evolution of patients with DR in terms of both retinal neurodegeneration and pro-angiogenic shift, this latter indicated by the progressive protein expression of the pro-angiogenic isoform VEGF-A(164,) which can be sustained by the PKC beta II/HuR pathway acting at post-transcriptional level. (unisr.it)
  • Changes in the messenger RNA (mRNA) and protein expression of genes related to endoplasmic reticulum stress (activating transcription factor 4 (ATF4), inositol-requiring protein 1 (IRE1), c-Jun N-terminal kinase (JNK), and C/EBP homologous protein (CHOP)) and apoptosis (B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax), Caspase-3, Caspase-9, and Caspase-12) were also observed. (bvsalud.org)
  • In normal lung (NF) and IPF-derived fibroblasts (FF) VEGF-A xxx a protein expression was upregulated by hypoxia, mediated through activation of VEGF-A xxx a gene transcription. (biomedcentral.com)
  • In contrast, hyperoxia up-regulated panVEGF-A and VEGF-A xxx a mRNA levels in FF but had no significant effect on NF or FF VEGF-A protein expression. (biomedcentral.com)
  • It also regulates HIF-1α and VEGF expression, presumably by inhibiting the MAPK signaling cascade. (wikipedia.org)
  • Recently, we reported that nuclear β-catenin, but not HIF-2α, regulates the expression of fibronectin and α-SMA in atmospheric oxygen. (molvis.org)
  • Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex which regulates systemic, local and intracellular homeostatic responses elicited by hypoxia. (enzolifesciences.com)
  • may be the main kinase, which proportionally regulates HIF-1 and IL-1 expression unbiased of ERK activation inversely. (moonphase2018.com)
  • In this study, we investigated the effects of TNF-α and propofol on occludin expression in human cerebral microvascular endothelial cell line, D3 clone (hCMEC/D3 cells), and explored the underlying mechanisms. (researchsquare.com)
  • Blood-brain barrier (BBB) is a multicellular structure and mainly consists of microvascular endothelial cells, astrocytes, pericytes, intercellular tight junctions and basal lamina [ 1 ]. (researchsquare.com)
  • Cerebral microvascular endothelial cells are linked together through the interactions among multiple tight junction proteins including claudins, occludin and ZO-1 [ 5 ]. (researchsquare.com)
  • It has been reported that TNF-α could decrease the expression of occludin in endothelial cells [ 13 ]. (researchsquare.com)
  • 1 They also stabilize endothelial and retinal pigment epithelial tight junctions and restore the integrity of blood retinal barrier. (dovepress.com)
  • VEGF protein is a potent proangiogenic factor that binds to its receptor on endothelial cells. (czytelniamedyczna.pl)
  • Estrogen modulation of HIF-1 and subsequent effects on endothelial cells is dependent on the Akt/PI3K pathway and protein synthesis as validated by the use of the inhibitors wortmannin and cycloheximide which abrogated estrogen's effects respectively. (biomedcentral.com)
  • STAT3 disruption also suppresses endothelial HDAC9 and blocks CM-induced HDAC9 expression, whereas HDAC9 re-expression restores CM-enhanced endothelial proliferation. (oncotarget.com)
  • This receptor complex has increased VEGF signalling activity in endothelial cells. (vegfr-3inhibitor.com)
  • 1] Diagnosed in 50% of patients with VHL disease, these hemangiomas are composed of endothelial cells and pericytes. (medscape.com)
  • Upregulation of HIF-1 expression may play a neuroprotective role in animal models of focal cerebral ischemia ( 6 ). (spandidos-publications.com)
  • In the present study, we investigated whether TSA treatment increases HIF-1α stabilization via acetylation under normoxic conditions, which would lead to VEGF upregulation and resistance to anticancer drugs. (oncotarget.com)
  • Upregulation of HIF-1a in all CLL cases suggests localized tissue hypoxia as an important stimulant of microvessel proliferation. (biomedcentral.com)
  • Kay et al report increased VEGF and bFGF in the supernatant of CLL cells grown in vitro and upregulation of mRNA encoding VEGF and its receptors and bFGF, suggesting that angiogenic factors are important in the biology of the malignant B-cell clone [ 10 ]. (biomedcentral.com)
  • The decreased expression of tight junction proteins, especially occludin, correlates with BBB disruption in several situations such as cerebral ischemic injury [ 7 ]. (researchsquare.com)
  • The aim of the present study was to analyze the cell viability-, migration and invasion, as well as the expression of proangiogenic proteins VEGF and HIF-1 alpha, in HepG2 hepatocarcinoma cells, following treatment with melatonin. (fapesp.br)
  • The expression of proangiogenic proteins VEGF and HIF-1 alpha, under conditions of normoxia and hypoxia, was verified using immunocytochemistry and quantified by densitometry. (fapesp.br)
  • Fibronectin (FN) was the only of the tested matrix proteins that induced an increase of VEGF secretion. (uni-marburg.de)
  • XAV932 was used to assess the suppression of nuclear β-catenin and its downstream effect on EMT marker proteins and VEGF expression. (molvis.org)
  • Treatment with XAV932 significantly reduced the level of nuclear β-catenin, but the levels of neither the EMT marker proteins nor VEGF were changed. (molvis.org)
  • In marked contrast, data from the hypoxic condition clearly establish that nuclear β-catenin plays little apparent role in the expression of EMT marker proteins. (molvis.org)
  • Instead, the loss of HIF-1α (but not HIF-2α) decreases the expression of the EMT marker proteins without sacrificing the levels of the prosurvival protein VEGF. (molvis.org)
  • The key regulator of this process, hypoxia inducible factor 1 (HIF-1), can initiate apoptosis by inducing high concentrations of proapoptotic proteins, such as BNIP3, and can cause stabilisation of p53. (bmj.com)
  • Collectively, these data support selective up-regulation of VEGF-A xxx a proteins in response to hypoxia, mediated in part through increased transcription. (biomedcentral.com)
  • Interestingly, estrogen is also capable of contributing to breast cancer progression by a novel role, via modulation of proteins involved in hypoxia signaling, namely hypoxia inducible factor 1 (HIF-1). (biomedcentral.com)
  • Real-time qRT-PCR, western blot and ELISA assays were used to measure the expression of HIF-1α and VEGF in McA RH-7777 cells under hypoxic conditions, respectively. (elis.sk)
  • Our findings showed that HIF-1α play an important role in the stimulation of the secreted VEGF expression under hypoxic conditions, suggesting that targeting both HIF-1α and VEGF could represent a potential therapeutic strategy in combination with TACE in the treatment of liver tumors. (elis.sk)
  • Estrogen treated TG1-1 cells secreted higher levels of VEGF which were comparable to secreted levels from cells grown under hypoxic conditions. (biomedcentral.com)
  • It is assured that the spectrum and the extent of the integrin expression of cancer cells correlate with their abilities of proliferation and invasion. (uni-marburg.de)
  • Consistent with our hypothesis, carnosine supplementation augmented HIF-1α and VEGF expression in the ischemic limb and the mobilization of proangiogenic Flk-1 + /Sca-1 + cells into circulation. (frontiersin.org)
  • In the present study, we have investigated whether PACAP and VIP prevent retinal damage by modulating the expression of HIFs, VEGF, and its receptors. (uniroma5.it)
  • This effect is also related to a reduction of VEGF and its receptors expression. (uniroma5.it)
  • In recent years, the MAPK family has been found to be an important signal-regulating enzyme between cell surface receptors and determinants of gene expression ( 8 ). (spandidos-publications.com)
  • The examination of the influence of VEGF on the integrin expression of the XTC cells in the sense of an "Inside-out" control loop could not prove an autocrine control loop in spite of the well known close interaction of integrins and VEGF receptors. (uni-marburg.de)
  • VEGF-A receptors and co-receptors were differentially expressed by hypoxia and hyperoxia. (biomedcentral.com)
  • The presence of VEGF receptors on CLL cells implies an autocrine effect for VEGF. (biomedcentral.com)
  • In this study, we tested the hypothesis that carnosine protects against post ischemia by augmenting HIF-1α angiogenic signaling by Fe 2+ chelation. (frontiersin.org)
  • Collectively, these data suggest that carnosine promotes post ischemic revascularization via augmentation of pro-angiogenic HIF-1α/VEGF signaling, possibly by Fe 2+ chelation. (frontiersin.org)
  • Miconazole also exhibits anti-angiogenic activities, decreasing expression of HIF-1α and VEGF in breast cancer and glioma cells. (lktlabs.com)
  • This "angiogenic switch" [ 1 ] favors the production of new microvessels, thus facilitating tumor growth beyond 1-2 mm diameter, and metastasis of the malignant clone. (biomedcentral.com)
  • It lifts the mRNA levels of plasminogen activator (PA) and plasminogen activator inhibitor (PAI) to play a role of activation and regulation on PA and PAI-1, thus enhancing the activity of plasminogen activator and promoting the formation of new capillaries. (spandidos-publications.com)
  • HLE-B3 cells, maintained in a continuous hypoxic environment (1% oxygen), were treated with SB216763, a specific inhibitor of glycogen synthase kinase-3β (GSK-3β) catalytic activity. (molvis.org)
  • A hypoxia-inducible factor-1α (HIF-1α) translation inhibitor and an HIF-2α translation inhibitor were independently employed to evaluate the effect of hypoxia inducible factor inhibition on EMT marker protein and VEGF expression. (molvis.org)
  • In conjunction with SB216763, coadministration of an HIF-1α translation inhibitor, but not an HIF-2α translation inhibitor, markedly suppressed the expression of fibronectin and α-SMA without affecting VEGF levels. (molvis.org)
  • Using the murine mammary cell line TG1-1 we observed estrogen (E 2 ) lead to an up regulation of hypoxia inducible factor-1 (HIF-1), an effect abrogated by the anti-estrogen Fulvestrant and the HIF-1 inhibitor YC-1 (3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole) suggesting the interchangeability of hypoxia and estrogen mediated effects. (biomedcentral.com)
  • Since the blockade of integrins had no reducing effect on the high VEGF basic output of thyroid cancer cells, the regulation of the VEGF secretion is assumed to be very complex and does not only depend on integrin signalling. (uni-marburg.de)
  • The role of hypoxia-inducible factors-1 and -2 in the regulation of CXCL12 expression was examined using over-expression and short hairpin RNA knockdown constructs, electrophoretic mobility shift assays and chromatin immunoprecipitation. (haematologica.org)
  • dependent regulation of MGMT, highlighting the existence of a HIF-1? (omicsdi.org)
  • dependent regulation of MGMT and suggest that BMP2, by down-modulating the HIF-1? (omicsdi.org)
  • A VHL protein is mandatory for the process of hypoxia-inducible factor 1 (HIF-1) regulation (4). (czytelniamedyczna.pl)
  • VEGF-A is a target of the hypoxic response via its translational regulation by HIF-1α. (biomedcentral.com)
  • TSA enhanced total HIF-1α and VEGF-HRE reporter activity under normoxic conditions. (oncotarget.com)
  • These data demonstrate that HIF-1α may be stabilized and translocated into the nucleus for the activation of VEGF promoter by TSA-mediated acetylation at K674 under normoxic conditions. (oncotarget.com)
  • As high HIF-2alpha expression further correlates with disseminated disease as demonstrated in neuroblastoma, glioma, and breast carcinoma, we propose that targeting HIF-2alpha and/or the pseudo-hypoxic phenotype induced by HIF-2 under normoxic conditions has great clinical potential. (lu.se)
  • Cobalt Chloride induced hypoxic-like growth conditions in NF and FF cultures, evidenced by HIF-1α expression, which was absent in normoxic conditions. (biomedcentral.com)
  • The knockdown of HIF-1α or VEGF significantly reduced the expression of the secreted VEGF. (elis.sk)
  • More importantly, knockdown of both HIF-1α and VEGF resulted in the best effective inhibitory effect in VEGF expression, and in turn remarkably reduced the cell migration and invasion activity. (elis.sk)
  • Knockdown of HIF-2alpha in neuroblastoma and glioma tumor stem/initiating cells leads to reduced levels of VEGF and poorly vascularized, highly necrotic tumors. (lu.se)
  • PanVEGF-A and VEGF-A xxx a mRNA levels were significantly up-regulated in response to hypoxia in NF and FF, although the FF response to hypoxia was significantly less than NF (Fig. 1a ). (biomedcentral.com)
  • Disruption of PERK activity has been demonstrated to reduce hydroquinone-induced apoptosis and hypoxia-induced VEGF expression in human RPE cells in vitro ( 5 , 21 ). (spandidos-publications.com)
  • CSCs) GBM cells were resistant to Temozolomide (TMZ) in vitro, owing to a particularly high expression of O6-methylguanine-DNA-methyltransferase (MGMT), the most important factor associated to therapy resistance in GBM. (omicsdi.org)
  • When cells were transfected with GFP-HIF-1α, treatment with TSA increased the number of green fluorescence protein (GFP)-positive cells. (oncotarget.com)
  • The cells were transfected fa There by stable or transient expression vectors which human bcl weight 2 or bcl two different mutants. (cd177signaling.com)
  • Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism. (nih.gov)
  • Ketoconazole and miconazole are antagonists of the human glucocorticoid receptor: consequences on the expression and function of the constitutive androstane receptor and the pregnane X receptor. (lktlabs.com)
  • Sunitinib, sorafenib and pazopanib are small molecules that inhibit several protein kinases including tyrosine kinase of VEGF receptor. (czytelniamedyczna.pl)
  • We investigated the effect of hypoxia and hyperoxia on VEGF receptor, co-receptor and VEGF-A isoform expression in fibroblasts explanted from histologically normal lung (normal fibroblasts, NF) compared to those from IPF tissue (fibrotic fibroblasts, FF). (biomedcentral.com)
  • This process activates protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK), inositol-requiring kinase 1 and activating transcription factor (ATF) 6, and initiates unfolded protein response (UPR) signaling pathways ( 13 , 14 ). (spandidos-publications.com)
  • In fact, excessive hypoxia and failure to upregulate phosphoglycerate-kinase 1 (PGK1), a key enzyme of anaerobic glycolytic metabolism, were among the earliest manifestations of HIF-1α deficiency in cartilaginous bone templates, and reduced PGK1 expression was irrespective of transgenic VEGF164. (ox.ac.uk)
  • In recent years, high tumor cell levels of HIF-2 and the oxygen sensitive subunit HIF-2alpha have been associated with unfavorable disease and shown to be highly expressed in tumor stem/initiating cells originating from neuroblastoma and glioma, respectively. (lu.se)
  • HIF-1 is also a heterodimeric transcription factor, consisting of the oxygen dependent alpha subunit and the constitutively expressed beta subunit. (biomedcentral.com)
  • The incubation of the two cancer cell lines (XTC, HTC+) without matrix components with a choice of the best examined and available integrin antibodies at this time had no effect on the VEGF secretion. (uni-marburg.de)
  • The FN specific integrin antibodies had no influence on the VEGF secretion. (uni-marburg.de)
  • Our data suggest that Eag1 interferes with the cellular mechanism for maintaining oxygen homeostasis, increasing HIF-1 activity, and thereby VEGF secretion and tumor vascularization. (mpg.de)
  • Collagen alpha-1(IV) chain (COL4A1) is a protein that in humans is encoded by the COL4A1 gene on chromosome 13. (wikipedia.org)
  • The VEGF-A gene is differentially spliced to produce several functioning isoforms, the subscript number denoting the number of amino acids in the protein [ 3 ]. (biomedcentral.com)
  • Since the RGDS led to an increase of the VEGF concentration and not to a suppression, as expected, it must be discussed, that they dispose of an intrinsic activity with initiation of intracellular signal pathways in the sense of an "Outside-in" signalling. (uni-marburg.de)
  • Here, we assessed whether VEGF is a critical downstream component of the HIF-1α-dependent survival pathway in chondrocytes. (ox.ac.uk)
  • Ether-a-go-go-1 (Eag1) is a CNS-localized voltage-gated potassium channel that is found ectopically expressed in a majority of extracranial solid tumors. (mpg.de)
  • In the absence of VHL protein, alpha subunits of HIF-1 are constitutively stabilized despite the normal oxygenation. (czytelniamedyczna.pl)
  • Cells will adapt by activating the expression of genes involved in metabolic adaptation, such as those involved in glycolysis. (bmj.com)
  • Together, the results of the current study suggest that the protective effect of vitexin reduces sevoflurane‑induced neuronal apoptosis through HIF‑1α‑, VEGF‑ and p38‑associated signaling pathways in newborn rats. (spandidos-publications.com)
  • These findings suggest that HIF-1α acetylation may lead to resistance to anticancer therapeutics, such as HDAC inhibitors, including TSA. (oncotarget.com)
  • These findings provide a new link in the mechanistic understanding of TMZ resistance under glioma hypoxia microenvironment, and consequently HIF-1? (omicsdi.org)
  • These findings suggest that HIF-1α activates VEGF-independent cell-autonomous mechanisms to sustain oxygen levels in the challenged avascular cartilage by reducing oxygen consumption. (ox.ac.uk)
  • HIF-1beta is constitutively expressed and serves as a dimerization partner for several other transcription factors, while HIF-1alpha and the closely related HIF-2 are unique to HIF-1 and their expression is tightly regulated by cellular oxygen concentration. (enzolifesciences.com)
  • We then explored the effect of hypoxia and hyperoxia on VEGF-A isoform expression. (biomedcentral.com)
  • Neuroblastoma tumor initiating cells are immature with a neural crest-like phenotype and downregulation of HIF-2alpha in these cells results in neuronal sympathetic differentiation and the cells become phenotypically similar to the bulk of neuroblastoma cells found in clinical specimens. (lu.se)
  • The survival rates of tumor patients are decreasing because of tumor cell resistance to anticancer chemotherapies [ 1 ]. (oncotarget.com)
  • Two cancer cell lines (XTC, HTC+) were incubated on different matrix components and the VEGF expression was examined via ELISA. (uni-marburg.de)
  • Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of VEGF in cell culture medium. (molvis.org)
  • Western blot analysis of lysates from (A) cobalt chloride treated HeLa cells and (B) untreated HeLa cells, probed with HIF-2α pAb. (enzolifesciences.com)
  • Transwell assays were performed to examine the effect of HIF-1α and VEGF knocking-down on McA RH-7777 cells migration and invasion. (elis.sk)
  • Importantly, we correlated these effects to a BMP2-induced downregulation of both hypoxia-inducible factor-1? (omicsdi.org)
  • Ischemia-reperfusion injury occurs when circulation is abruptly restored following prolonged ischemia and it is well-known that high levels of calcium and tissue neutrophil accumulation cause cellular damage and produce reactive oxygen species (ROS) during reperfusion and trigger IR injury [ 1 ]. (hindawi.com)
  • The RGD sequences increased the intra- and extra cellular VEGF concentration of the XTC cells proportional to the RGDS-concentration. (uni-marburg.de)
  • The VEGF-synthesis of thyroid cancers seems thus to be influenced by the extra cellular matrix (FN) via integrins. (uni-marburg.de)
  • Cellular adaptation to diminished tissue oxygen tensions, hypoxia, is largely governed by the hypoxia inducible transcription factors, HIF-1 and HIF-2. (lu.se)
  • inbook{ecf3c49f-5038-4d94-a89d-062e1056050f, abstract = {{Cellular adaptation to diminished tissue oxygen tensions, hypoxia, is largely governed by the hypoxia inducible transcription factors, HIF-1 and HIF-2. (lu.se)
  • Similarly, NF and FF VEGF-A xxx b mRNA (data not shown) and VEGF-A 165 b protein (Fig. 1b ) were not significantly altered in response to hypoxia or hyperoxia. (biomedcentral.com)
  • Yet, similarly crossed double-mutant embryos lacking HIF-1α and overexpressing VEGF164 in the growth plate cartilage still displayed a central cell death phenotype, albeit slightly delayed and less severe compared with mice exclusively lacking HIF-1α. (ox.ac.uk)
  • While the mechanisms responsible for aberrant plasma cell expression of CXCL12 remain to be determined, studies in other systems suggest a role for hypoxia and hypoxia-inducible transcription factors. (haematologica.org)
  • 12 It is well established that hypoxia is an important selective force in the evolution of tumor cells, 13 and elevated expression of the hypoxia-inducible transcription factors HIF-1 and HIF-2 has been documented in several human cancers. (haematologica.org)
  • The plasma levels of inflammatory factors such as TNF-α, IL-1β and IL-6 were raised in perioperative period. (researchsquare.com)
  • In contrast, pretreatment of hypoxic C2C12 cells with methylcarcinine, a carnosine analog, lacking Fe 2+ chelating capacity, had no effect on HIF-1α levels and VEGF release. (frontiersin.org)
  • ATF4, CHOP and VEGF mRNA expression levels were assessed using reverse transcription‑quantitative polymerase chain reaction. (spandidos-publications.com)
  • Tumor hypoxia and high HIF protein levels are frequently associated with aggressive disease. (lu.se)
  • Plasma magnesium levels were available for 66 patients with chronic kidney disease (stages 1 to 3). (scite.ai)
  • Design and Methods The expression of hypoxia-inducible factor protein was examined in patients' bone marrow biopsy specimens using immunohistochemistry. (haematologica.org)
  • Prolonged exposure to hypoxia strongly up-regulated CXCL12 expression in multiple myeloma plasma cells and hypoxia-inducible factor-2 was found to play a key role in this response. (haematologica.org)
  • Numerous clinical and experimental studies demonstrated that microcirculatory disturbance may lead to flap failure 1 1. (scielo.br)
  • The time-dependent VEGF-A(164) expression in the retina of diabetic Ins2 ( Akita ) mice suggests that pharmacological intervention in DR might be chosen, among other reasons, on the basis of the specific stages of the pathology in order to pursue the best clinical outcome. (unisr.it)
  • Carnosine (β-alanine-histidine) is an endogenous histidyl dipeptide, present in high concentration in the skeletal muscle (1-20 mM), heart (0.1-1 mM), and brain (1-2 mM). (frontiersin.org)
  • Carnosine supplementation enhanced the bioavailability of carnosine in the ischemic limb, which was accompanied by increased expression of proton-coupled oligopeptide transporters. (frontiersin.org)
  • A wiser therapeutic strategy may therefore be to consider the use of drugs or drug combinations that have multiple pathogenic targets, with minimal o-target and negligible peripheral toxic effects [1-7,32,109-114]. (c-kitinhibitors.com)
  • The enhancement of nuclear β-catenin looked as if it positively correlated with a significant increase in the basal expression of VEGF as well as increased expression of fibronectin and α-SMA. (molvis.org)
  • In accord to our hypothesis, we have shown that both peptides are able to significantly reduce HIF-1α and increase HIF-3α expression in ARPE-19 cells exposed to hyperglycemic/hypoxic insult. (uniroma5.it)
  • In addition, the inhibitors of Hif-1α, VEGF, VEGFR-2, and ERK could reduce the effect of TNF-α on occludin expression. (researchsquare.com)
  • In this study, we sought to explore whether propofol could attenuate the effect of TNF-α on occludin expression and identify the underlying mechanisms. (researchsquare.com)
  • In a further examination of XTC cells, integrin antibodies and integrin blocking RGD sequences (RGDS) were used to suppress the stimulating effect of FN on VEGF. (uni-marburg.de)
  • Effect of short-term ischemia on microcirculation and wound healing of adipocutaneous flaps in the rat 1 1 Research performed at Department of Operative Techniques and Surgical Research, Institute of Surgery, Faculty of Medicine, University of Debrecen, Hungary. (scielo.br)
  • We report here a novel mechanism involving the HIF-1? (omicsdi.org)