Expression of cd24 and cd44. Medical search. Frequent questions

CAE induced a dose-dependent increase in cell migration and invasion and also in the expression of cancer stem cells markers (CD133, CD44, CD90). (springer.com)
The objective of this article was to evaluate how these chemotherapeutic regimens affect the proportion of cancer stem cells and the expression of stemness markers. (isciii.es)
After analyzing different tumor stem cell markers (SOX2, OCT4, CD133, CD44 and CD24) in pancreatic cancer cells treated with different chemotherapeutic protocols by means of RT-qPCR, Oxaliplatin and Gemcitabine in monotherapy were the chemotherapies that selected the most cancer stem cells while the FOLFIRI protocol decreased them. (isciii.es)
The expression of the different markers also changes depending on the treatment used. (isciii.es)
The underlying mechanism revealed significant downregulation of β-catenin and the set of CSC markers along with their reduced mRNA expression in secondary sphere culture. (nih.gov)
To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. (hindawi.com)
On the other hand, in brain tumors, such as glioma and glioblastoma, CSCs have instead been identified using cell surface markers, including SSEA-1 20 , EGFR 21 , and CD44 22 . (atlasantibodies.com)
For lung cancers, CSC markers include CD44 and CD133 (PROM1), but also CD117 (KIT), CD90 (or THY1), CD166, EpCAM for non-small-cell lung carcinoma (NSCLC), and PODXL-1, PTCH, and CD87 for small-cell lung carcinoma (SCLC) 25 . (atlasantibodies.com)
Because of their unique expression profile and special functions, miRNAs have been used as molecular markers to define the direction and processes of cell differentiation by regulating one or several target genes. (researchsquare.com)
Aims: To determine the validation of the CSC model for cell surface markers such as CD44 and CD24 and their clinical significance. (nbuv.gov.ua)
For the first time, we employ this new approach to query CTCs with single-cell resolution in accordance with their expression of phenotypic surface markers and migration properties, revealing the dynamic phenotypes and the existence of a high-motility subpopulation of CTCs in blood samples from metastatic lung cancer patients. (nsf.gov)
CD44 isoforms are heterogeneously expressed in breast cancer and correlate with tumor subtypes and cancer stem cell markers. (lu.se)
We demonstrate that individual CD44 isoforms can be associated to different breast cancer subtypes and clinical markers such as HER2, ER and PgR, which suggests involvement of CD44 splice variants in specific oncogenic signaling pathways. (lu.se)
The expression level of CD24 and CD44 as CSC markers was measured by flow cytometry in secondary mammospheres, and the expression of NANOG, SOX2, and OCT4 genes in CSCs was also analyzed using the real-time polymerase chain reaction (PCR). (celljournal.org)
Tanshinone IIA attenuated the stemness of breast cancer cells, evident by downregulating the expression of stemness markers, hindering the capacity of spheroid formation, decreasing the CD24 − /CD44 + sub-population in a concentration-dependent manner and reducing the tumor-initiating ability of breast cancer cells. (biomedcentral.com)
Expression profiling revealed 780 genes for which the expression level was affected by the loss of DIP2C, including the tumour-suppressor encoding CDKN2A gene, the epithelial-mesenchymal transition (EMT) regulator-encoding ZEB1, and CD44 and CD24 that encode breast cancer stem cell markers. (scilifelab.se)
Our results demonstrated that ETV2 overexpression significantly upregulated the mRNA and protein expression levels of osteogenic markers, markedly enhanced ALP activity, and promoted matrix mineralization of hDPSCs. (biomedcentral.com)

The effects of doxorubicin (DOX) and paclitaxel on sheared cells using WST8 assay and stemness (CD44 + /CD24 − ) and apoptosis (Annexin V + /7‐AAD + ) using flow cytometry are tested. (nsf.gov)
Breast cancer stem cells (CSCs) isolated from the tumors were determined by flow cytometry analysis using CD44 + /CD24 - or low. (biomedcentral.com)
Flow cytometry analysis showed that cells with CD24- CD44+ phenotype were enriched in the culture period (from 2.6% on day 1 to 32.6% on day 22). (celljournal.org)
In vitro mammary spheroid formation, flow cytometry assay on CD24 − /CD44 + sub-population, ALDH activity detection, cell viability assay and western blot analysis, and in vivo tumor-initiating analysis were performed to examine the effects of Tanshinone IIA on the stemness of breast cancer cells. (biomedcentral.com)

Tras analizar mediante RT-qPCR diferentes marcadores de células madre tumorales (SOX2, OCT4, CD133, CD44 y CD24) en células de cáncer de páncreas tratadas con diferentes protocolos quimioterapéuticos, el Oxaliplatino y la Gemcitabina en monoterapia fueron los quimioterápicos que seleccionaron en mayor medida las células madre cancerosas mientras que el protocolo FOLFIRI las disminuyó. (isciii.es)
These findings link iNOS to Notch1 signaling in CD24(+)CD133(+) LCSCs through the activation of TACE/ADAM17. (cusabio.com)

Expression levels of CD44v6 and ALDH1A1 were significantly increased in cancer cells of primary NPC specimens in comparison to chronic nasopharyngitis tissues. (hindawi.com)
This being true, ALDH1A1 RNA expression is significantly associated with poor prognosis. (bvsalud.org)
On the contrary, aldehyde dehydrogenase 1A1 (ALDH1A1) has emerged as an excellent marker to identify CSCs, owing to its conserved expression profile in nearly all progenitor cells including CSCs. (bvsalud.org)

In addition, the expression of stemness genes/proteins is also used to identify CSCs. (atlasantibodies.com)
Open in a separate window Number 4 The addition of hyperthermia to radiation significantly reduced cell proliferation and viability in breast CSCs and pancreatic CSCs(A) The surviving fraction of CD44+CD24C CSCs based on cell number counting at 24, 48, 72 hours after indicated treatments. (monossabios.com)
B) The surviving fraction of CD44+CD24+ESA+ CSCs based on cell number counting at 72 hours after indicated treatments. (monossabios.com)
C) The surviving fraction of CD44+CD24C CSCs based Abiraterone kinase activity assay on cell viability was recognized by CCK-8 at 48 hours post indicated treatments. (monossabios.com)
We aimed to investigate potential associations between alternatively spliced isoforms of CD44 and CSCs as well as to various breast cancer biomarkers and molecular subtypes. (lu.se)
Efforts to link CD44 to CSCs and tumor progression should consider the expression of various CD44 isoforms. (lu.se)
These findings support the hypothesis that the possibility should be considered of sunitinib increasing breast CSCs though it inhibits TNBC tumor angiogenesis and growth/progression, and that effects of sunitinib on Notch expression and hypoxia may increase breast cancer stem cells. (biomedcentral.com)
Conclusion: Altogether, we found that heat-induced upregulated expression of gp96 in CSCs enriched mammospheres from breast tumor tissue might be used as a complementary procedure to generate more immunogenic antigens in immunotherapy settings. (celljournal.org)

In the present study, we revealed that microRNA-30 (miR-30) members, especially miR-30b, were remarkably downregulated in triple-positive (CD24 + , CD44 + , EpCAM + ) pancreatic cancer stem cells (PCSCs). (jcancer.org)

For instance, Zhang et al ( 23 , 24 ) found that RUNX2 was required for the activity of CD44 + /CD24 −/low BCSCs during breast cancer development, while miR-205/RUNX2 axis was further identified with s negative regulatory effect upon the activity of CD44 + /CD24 − BCSCs. (spandidos-publications.com)
The miRNA profile between breast cancer stem cells(BCSCs, CD44+CD24-/low) and control MCF-7 breast cancer cells was obtained in a previous study. (researchsquare.com)
Previous studies have confirmed that miRNAs play an important role in gene regulation in CD44 + CD24 -/low breast cancer stem cells (BCSCs). (researchsquare.com)
In addition, let-7a plays an important role in BCSCs' self-renewal by inhibiting the expression of H-Ras [11, 12]. (researchsquare.com)
In our previous research, we performed miRNA profiling between sorted CD44 + CD24 -/low BCSCs and the control MCF-7 breast cancer cells [8]. (researchsquare.com)
In vitro results demonstrated that ASR490 significantly inhibited BCSCs (ALDH + and CD44 + /CD24 - ) and breast cancer (BC) growth at nM concentrations, and subsequently inhibited the colony- and mammosphere-forming abilities of BCSCs and BCs. (figshare.com)

whereas human epidermal growth factor receptor 2 (HER2)-positive breast cancers can be targeted by specific drugs, the triple-negative breast cancer phenotype is defined by chromosomal instability and low expression levels of estrogen receptor (ER) alpha, progesterone receptor (PR), and HER2, rendering treatment rather difficult. (dovepress.com)
Taken together, these findings implicate that miR-30b may suppress PCSC phenotype and PDAC metastasis through posttranscriptionally suppressing Snail expression, highlighting that miR-30b may serve as a therapeutic agent in the treatment of PDAC. (jcancer.org)
Tumors characterized as positive for the CD44+/CD24- phenotype by immunohistochemistry were associated to all isoforms except the CD44 standard (CD44S) isoform, which lacks all variant exons. (lu.se)

ALDH1 protein expression was determined by IHC on TMA. (lu.se)
Conversely, tumors with strong expression of the CSC marker ALDH1 had elevated expression of CD44S. (lu.se)

Taken together, sorted CD133+ cells or CD44+CD24+ESA+ cells showed the enhanced tumorigenic potential, improved manifestation of the stemness related molecule Sox2 and highly resistant to radiation, showing their stem cell properties as earlier works reported. (monossabios.com)
Real-time PCR indicated that OCT4, NANOG, and SOX2 expression in mammospheres were increased by 3.8 ± 0.6, 17.8 ± 0.6, and 7.7 ± 0.8 fold respectively in comparison to the MCF-7 cell line. (celljournal.org)

The target genes were predicted by the online programs picTar, miRanda and TargetScan and verified by dual luciferase assay, and changes in protein expression were detected by western blot. (researchsquare.com)
Using tumorsphere formation assay we demonstrated that PRL was able to alter the cancer cell proliferation pattern in tumorspheres, which accompanied with increased CD44 + 24 - cell population. (ommegaonline.org)
and identified by surface marker expression of CD44 + /CD24 - /lin -[5] and can be even visualized with tumorsphere assay [6] . (ommegaonline.org)
To find the optimal temperature regulation of gp96, the mammosphere was incubated at different temperatures for 1 hour, and gp96 expression was measured using the western blotting assay. (celljournal.org)

Conclusions: As a result of our research, the analysis of the quantity of assumed stem cells of the BC, which were identified by immunohistochemistry as CD44 and CD24 cells, failed to detect a statistically significant relation between groups of patients with different prognosis, and the identification of their expression is not enough for the characteristics of CSC. (nbuv.gov.ua)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA254759(CD24 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (cusabio.com)
The circBACH1 and miR-217 expression levels were detected using quantitative real-time PCR (RT-qPCR) and Immunohistochemistry (IHC) assays in BC tissues and precancerous tissues of BC patients. (biomedcentral.com)

G7mAb was an anti-CD24 antibody. (cusabio.com)

Expression of CD44s, the most common CD44 isoform, has been reported to be associated with increased survival of patients with nodenegative invasive breast carcinoma (3). (biocare.net)
A high expression of the CD44v2-v10 isoform, which retain all variant exons, was correlated to positive steroid receptor status, low proliferation and luminal A subtype. (lu.se)
The CD44v3-v10 isoform showed similar correlations, while high expression of CD44v8-v10 was correlated to positive EGFR, negative/low HER2 status and basal-like subtype. (lu.se)
Unsupervised hierarchical cluster analysis of CD44 isoform expression data divided tumors into four main clusters, which showed significant correlations to molecular subtypes and differences in 10-year overall survival. (lu.se)

Ectopic expression of AUF1 also promoted stemness in mammary epithelial cells, and thereby increased the proportion of cancer stem cells. (nature.com)
A known combination marker of cancer stem cells is high expression of CD44 and low expression of CD24 surface proteins. (weeksmd.com)
Are CD44⁺/CD24⁻ cells the assumed cancer stem cells in breast cancer? (nbuv.gov.ua)
A group of cells with high expression of CD44 high /CD24 low or ALDH + in breast cancer are considered to be breast cancer stem cells (BCSC) [ 6 ] . (ijbs.com)
CD24, a cell surface receptor enriched in both juvenile chondrocytes and human induced pluripotent stem cells-derived chondrocytes, is a regulatory factor in both faster proliferation and resistance to proinflammatory cues in these chondrocyte populations. (cusabio.com)

Comparing gene expression between GD2+ cells and CD44 high/CD24 low cells revealed 100 percent correlation in the expression of 231 genes. (weeksmd.com)
In our study, two unsupervised knowledge-based matrix factorization methods, independent component analysis (ICA) and nonnegative matrix factorization (NMF) are integrated to identify significant genes and related pathways in microarray gene expression dataset of Alzheimer's disease. (biomedcentral.com)
For malignant cells, the expression of these marker genes is highly consistent with the karyotypes of malignant and non-malignant cells (Figure 1E). (medicaltrend.org)
Changes in DNA methylation at promoter regions were strongly correlated to changes in gene expression, and genes involved with EMT and cell death were enriched among the differentially regulated genes. (scilifelab.se)

An analysis of 12 human breast cancer tumors found an even higher correlation of 95.5 percent between GD2+ cells and CD44 high/CD24 low status. (weeksmd.com)
Studies have shown that CD44, also known as HCAM, plays an important role in the cascade of metastasis and progression of human malignant tumors (2). (biocare.net)
The CD44 cell adhesion molecule is aberrantly expressed in many breast tumors and has been implicated in the metastatic process as well as in the putative cancer stem cell (CSC) compartment. (lu.se)
We used q-RT-PCR and exon-exon spanning assays to analyze the expression of four alternatively spliced CD44 isoforms as well as the total expression of CD44 in 187 breast tumors and 13 cell lines. (lu.se)
High expression of CD44S was associated with strong HER2 staining and also a subgroup of basal-like tumors. (lu.se)

ADH activity and ALDH expression were much lower in SNU449 compared with Huh-7 cells and at the 270 mM dose, CAE decreased cell viability by about 50% and 80%, respectively, in Huh-7 and SNU449 cells but induced mortality only in Huh-7 cells. (springer.com)
They found that GD2 expression, one such mesenchymal stem cell marker, divided the breast cancer cell lines into two distinct groups: about 4.5 percent of cells were GD2-positive and about 92.7 percent were GD2-negative. (weeksmd.com)
The researchers found 85 percent of GD2-positive breast cancer cells were CD44 high/CD24 low, while only 1 percent of GD2-negative cells shared that characteristic. (weeksmd.com)
In conclusion, the current study revealed the expression profiles of human TRP channels in 60 different breast cancer tissues and cell lines and furthermore validated the antitumor activity of TRPV1 against SUM149PT breast cancer cells, indicating that activation of TRPV1 could be used as a therapeutic target, even in the most aggressive breast cancer types. (dovepress.com)
CD44 and CD24 antigens expression was evaluated by the percentage of positive cells using different chromogens and the MultiVision detection system by immunohistochemical method. (nbuv.gov.ua)
positive - expression in ≥10% of cells. (nbuv.gov.ua)
The content of CD44 cells did not differ statistically between groups A and B (p = 0.18), but there was a tendency for increasing in OS with the existence of CD44+ cells (p = 0.056). (nbuv.gov.ua)
Further studies have shown a subpopulation of CD44+/CD24- in breast cancer cells have stem/progenitor cell properties (4). (biocare.net)
Here, KLF5 was proven to be a direct transcription factor for EphA2 in BLBC cells, and its expression was positively correlated in clinical samples from breast cancer patients. (ijbs.com)
For CD44+CD24+ESA+ cells, is definitely 0.148, is 0.045, mean surviving fraction at 2 Gy [SF2Gy] = 0.62. (monossabios.com)
The results showed that CD44+CD24+ESA+ cells were more radioresistant than L3.6pl cells. (monossabios.com)
There is a synergistic increase in the CD44 + /CD24 − CSC‐like population and an increase in drug resistance‐related gene expression in MCF7 cells co‐treated with FSS and drugs. (nsf.gov)
Breast cancer cell lines showed a heterogeneous expression pattern of the CD44 isoforms, which shifted considerably when cells were grown as mammospheres. (lu.se)
CircBACH1 combined miR-217 cotransfection suppressed the expression of G3BP2 proteins compared with circBACH1 treatment in MCF-7 cells. (biomedcentral.com)
These results demonstrated that PTX-induced exosomal circBACH1 promoted stemness and migration of BC cells by sponging miR-217 to upregulate the expression of G3BP2, which provided a new therapeutic target for PTX-resistance and progression of BC via circBACH1/miR-217/G3BP2 axis. (biomedcentral.com)
The effect of sunitinib on Notch-1 expression was determined by Western blot in cultured MDA-MB-468 cells. (biomedcentral.com)
Sunitinib significantly increased the expression of Notch-1 protein in cultured MDA-MB-468 or MDA-MB-231 cells. (biomedcentral.com)
In the short-term AN model, iPS-MSCs conferred a substantial anti-apoptotic effect on tubular cells, associated with a downregulation of Bax and Bax/Bcl2 ratio and an upregulation of survivin expression. (oncotarget.com)
The previous work has shown that Tanshinone IIA could ameliorate inflammation microenvironment in colorectal cancer via repressing miRNA-155 expression ( 8 ) and exert an anti-angiogenic effect in vascular endothelial cells via inhibiting the VEGF/VEGFR2 pathway [ 9 ]. (biomedcentral.com)
Loss of DIP2C triggers substantial DNA methylation and gene expression changes, cellular senescence and epithelial-mesenchymal transition in cancer cells. (scilifelab.se)

In vitro , conditioned medium from iPS-MSCs (iPSMSC-CM) significantly limited albumin-induced tubular apoptosis and enhanced tubular proliferation, accompanied by a reduced expression of tubular Bax and an elevated expression of Bcl2 and survivin. (oncotarget.com)

2 - 4 Expression profiling on a genomic scale led to the classification of the intrinsic subtypes of breast cancer, thus allowing for the prediction of disease progression and patient stratification. (dovepress.com)

We believe that CD24 is a key molecule of metastatic progression in the epithelial-mesenchymal-transition phenomenon and a promising therapeutic target for advanced ovarian cancer. (cusabio.com)

ASR490 downregulated the expressions of Notch1 intracellular domain (NICD: The active form of Notch1) and its downstream effectors Hey1 and HES1. (figshare.com)

CD44 is a transmembranous glycoprotein (80 kDa) responsible for cell adhesion that is expressed in a wide range of normal tissues, such as tonsil, breast, prostate, skin, bladder and cervical squamous epithelium (1). (biocare.net)
It was noted that in clinical samples of breast cancer, ALDH + accounted for 39.4% in samples of BLBC, and similar results were obtained in studies on breast cancer cell lines [ 7 ] , while lower expression was observed in intraductal carcinoma and luminal type with better prognosis [ 8 ] . (ijbs.com)
To clarify the cell-autonomous defect in developmental processes, we here performed transcriptome analyses of isolated embryonic motor neurons of SMA model mice to explore mechanisms of dysregulation of cell-type-specific gene expression. (bvsalud.org)
Effects on cell death pathways and epithelial-mesenchymal transition traits were studied based on the results from expression profiling. (scilifelab.se)

Our results suggest that higher CD24 expression is significantly associated with lower OS rate, lower DFS rate and some clinicopathological factors such as lymph node invasion and TNM stage. (cusabio.com)
Because of discrepancies in the expression of results and their application to populations with varying risk potentials, DNA ploidy and LOH assays cannot be directly compared. (biomedcentral.com)

State‑of‑the‑art research has indicated the retention of RUNX2 expression in a more invasive subtype of breast cancer, and in particular, triple‑negative breast cancer development and drug resistance are associated with estrogen receptor signaling pathways. (spandidos-publications.com)

Notable downregulation of the enriched stemness of secondary mammosphere was detected by the significantly decreased percentage of CD44 + /CD24 -/low population after eugenol treatment along with their distorted morphology and smaller the number of spheres. (nih.gov)

Low level of miR-30b and high Snail expression both predict dismal prognosis in PDAC patients. (jcancer.org)
Modern signal processing and machine learning techniques provide new and efficient analysis tools to the high-throughput microarray gene expression dataset. (biomedcentral.com)
Clinically, tumor samples from breast patients with less response to neoadjuvant chemotherapy showed a high level of EphA2 pS897 expression. (ijbs.com)

Nevertheless, the comparison of the clinical outcomes did not reveal a statistically significant difference in the survival curves in the groups with existence and absence of CD44⁺/CD24⁻ expression (p = 0.08). (nbuv.gov.ua)
The obtained data demonstrating the worst clinical outcome in the cases of absence of CD44⁺/CD24⁻ expression apparently require further investigations and the validation of the immunohistochemical method with the determination of the cut-off line in defining of CD44 and CD24 status. (nbuv.gov.ua)

Conclusion: E-Cadherin expression is not stable during the metastatic process. (iiarjournals.org)

Overexpression of miR-30b led to reduced expression of mesenchymal marker N-cadherin and the upregulation of epithelial marker E-cadherin. (jcancer.org)

however, no study has examined the expression profiles of human TRP channels in breast cancer on a large scale. (dovepress.com)
Next-generation sequencing analyses revealed the expression of TRPV1 in several native breast cancer tissues, which was subsequently validated via reverse transcriptase-polymerase chain reaction. (dovepress.com)
In breast cancer studies, evaluation of CD44 expression has been useful in the differentiation of benign and malignant papillary lesions (2). (biocare.net)
It has been well-studied in primary cancer, but its expression after metastasis is not well-described. (iiarjournals.org)
CD24 and CD44 are upregulated in human pancreatic cancer compared to chronic pancreatitis and may be related to the development of pancreatic cancer. (cusabio.com)

There is also a correlated increase in STAT3 and NANOG expression under FSS. (nsf.gov)

Meanwhile, the dysregulation and alteration of RUNX2 expression or activity may result in arteriosclerosis, skeletal dysplasia (e.g., cleidocranial dysplasia) and tumorigenesis ( 4 , 12 - 14 ). (spandidos-publications.com)

Unsupervised machine learning approaches provide new and efficient analysis of gene expression profiles. (biomedcentral.com)

This combinatorial approach offers unique capability to profile CTCs on the basis of their surface expression and migratory characteristics. (nsf.gov)

This study focused on the expression and functionality of TRPV1, a nonselective cation channel that was found to be expressed in different carcinoma tissues. (dovepress.com)
In the case of CD24 staining, there was no significant difference between NPC and chronic nasopharyngitis tissues. (hindawi.com)

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