SuperoxideOxidative stress and increasedMRNA expressionApoptosisIntracellularProteinGene ExpressionAssayEnzymaticPeroxidaseEnzymeLevelsCloningModulationTissuesGenesReductionCharacterizationConcentrationEndothelial cellsSignificantActivityRecombinantActivitiesTumorSpeciesHumanHydrogenInducesVivoLiverMiceVectorJournalTolerancePatternsFoundYieldMaximumHighest
Superoxide14
- Superoxide dismutase 2 (SOD2) gene expression was upregulated in WW, MeDM + VDK, and RW. (whiterose.ac.uk)
- IL-1β activated superoxide dismutase 2 and inhibited catalase, thereby modulating intracellular levels of ROS and inducing ICAM1 expression. (nih.gov)
- Malondialdehyde level, catalase and superoxide dismutase activities were determined using kits. (ijpsonline.com)
- Activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) were measured in ellagic acid-treated V79-4 cells. (nutrimedical.com)
- In heart, low vitamin E and C levels (300 and 70 mg kg‐−1, respectively) significantly increased superoxide dismutase and glutathione peroxidase expression and high addition increased the expression of tnfα and cox-2 (0 or 23 mg kg‐−1 K, 450 mg kg‐−1 E and 230 mg kg‐−1 C, diet EECC and KEECC). (stir.ac.uk)
- The activities and mRNA expression levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GRD) were determined in brain tissues. (uwi.edu)
- The beneficial effect of ASTA is associated with a reduction intracellular O2 (-) production by restoring the antioxidant network activity of superoxide dismutase (SOD) and catalase (CAT), which influence HO-1 expression and activity by inhibiting nuclear translocation of Nrf2. (nih.gov)
- Dietary Se, VE and Se + VE significantly enhanced the activities and mRNA levels of catalase as well as superoxide dismutase (SOD) but decreased the mRNA levels of HSP70 and HSP90 . (springeropen.com)
- Hydrogen-rich saline also increased B-cell lymphoma 2 (Bcl-2) expression and the activity of the antioxidant enzyme superoxide dismutase (SOD). (arvojournals.org)
- Inflammatory cytokines were evaluated using ELISA kits and oxidative stress markers were assessed using a malondialdehyde assay kit, superoxide dismutase assay kit and CATalase assay kit. (spandidos-publications.com)
- Under cold stress, D. versiformis inoculation did not change the activity of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in leaves and roots, whereas P. indica inoculation significantly increased the activity of CAT in roots and POD in leaves only. (techscience.com)
- SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. (thermofisher.com)
- Superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx) activities and uric acid (UA) levels were measured in plasma and saliva. (bvsalud.org)
- To counteract the oxidative stress mammalian cells have antioxidant system which includes enzymatic activities such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx), and non enzymatic antioxidants including vitamins A, E, C, melatonin, uric acid (UA) and glutathione 6,10 . (bvsalud.org)
Oxidative stress and increased2
- They show that loss of AIF leads to oxidative stress and increased levels of catalase and glutathione. (the-scientist.com)
- The supplementation of Trans-resveratrol attenuated the cardiac oxidative stress and increased the activity of catalase. (bvsalud.org)
MRNA expression2
Apoptosis1
- Genetic analysis revealed the presence of an ecotropic proviral insertion within rhe first intron of the gene encoding apoptosis-inducing factor (AIF) that results in a dramatic reduction in expression. (the-scientist.com)
Intracellular1
- IL-1β secreted by TAMs activates SOD2 and inhibits CAT to modulate intracellular ROS levels, thereby inducing ICAM1 expression. (jci.org)
Protein7
- Three-dimensional protein structures of the peroxidated catalase intermediates are available at the Protein Data Bank. (wikipedia.org)
- Recombinant expression of proteins of interest in Escherichia coli is an important tool in the determination of protein structure. (iucr.org)
- These data indicate that this eukaryotic cell-free expression system has a high success rate and that the addition of specific reagents can increase the yield of soluble protein. (iucr.org)
- Protein aggregation remains a significant problem in E. coli expression systems. (iucr.org)
- Conversely, HRS decreased Bcl2-associated X protein expression, cleaved caspase-3, and oxidant-stress product malondialdehyde (MDA) in a Sirt1-dependent manner. (arvojournals.org)
- The mRNA and protein expression levels of FOXA2 were determined by reverse transcription‑quantitative PCR and western blotting, respectively. (spandidos-publications.com)
- Western blotting was performed to examine the protein expression levels of endoplasmic reticulum stress (ERS)‑associated factors. (spandidos-publications.com)
Gene Expression7
- investigated the fungus at the level of gene expression, and performed proteome-level studies ( Yajima and Kav, 2006 ). (frontiersin.org)
- One important strategy for studying these dynamic but highly regulated host defenses is to observe gene expression patterns in the host 5 . (nature.com)
- Our results suggest that the association of low/moderate intake of alcohol beverages, with nutraceutical-proven effectiveness, and ethanol, in association with a Mediterranean diet, could determine a reduction of atherosclerosis risk onset through a positive modulation of antioxidant gene expression helping in the prevention of inflammatory and oxidative damages. (whiterose.ac.uk)
- Gene expression data reported significantly upregulation of apoptotic genes. (edu.sa)
- The data show that endogenous levels of H2S and ABA in maize seedlings could be mutually induced by regulating their metabolic enzyme activity and gene expression under non-heat stress (non-HS) and HS conditions. (bvsalud.org)
- In this study root-irrigation with 0.15 mM sodium nitroprusside (SNP, NO donor) up-regulated gene expression of L-CYSTEINE DESULFHYDRASE1 (LCD1), activities of L-cysteine desulfhydrase (LCD) and D-cysteine desulfhydrase (DCD), as well as an endogenous H2S level, compared to control seedlings. (bvsalud.org)
- NaHS had no significant effect on gene expression and activity of nitrate reductase (NR, a NO candidate producing enzyme). (bvsalud.org)
Assay1
- catalase functional activity in lysates was determined using a H 2 O 2 activity assay. (bmj.com)
Enzymatic1
- The lipid peroxidation (TBARS) and the enzymatic activity of catalase (CAT) were measured in the cardiac muscle. (bvsalud.org)
Peroxidase1
- In addition, Se and Se + VE significantly enhanced the glutathione peroxidase (GPx) activity and the expression of GPx1 and GPx4 in breast muscle tissues. (springeropen.com)
Enzyme4
- Catalase is a common enzyme found in nearly all living organisms exposed to oxygen (such as bacteria, plants, and animals) which catalyzes the decomposition of hydrogen peroxide to water and oxygen. (wikipedia.org)
- While the complete mechanism of catalase is not currently known, the reaction is believed to occur in two stages: H2O2 + Fe(III)-E → H2O + O=Fe(IV)-E(.+) H2O2 + O=Fe(IV)-E(.+) → H2O + Fe(III)-E + O2 Here Fe()-E represents the iron center of the heme group attached to the enzyme. (wikipedia.org)
- Therefore, we concluded that arbuscular mycorrhizal fungi (especially P. indica ) mainly induced the expression of antioxidant enzyme genes, depending on the fungal species, and thus mitigated oxidative damage for higher cold resistance in inoculated plants. (techscience.com)
- Furthermore, CagA-positive strains are known to induce the expression of a DNA-editing enzyme, which leads to accumulation of mutations in the tumor suppressor p53. (medscape.com)
Levels5
- The expression levels of LoCAT 1 in roots and stems of L. olgensis after the treatment of NaCl were down-regulated, with the lowest expression at 12 h. (nefu.edu.cn)
- The expression levels of LoCAT 1 in roots and stems of L. olgensis after the treatment of PEG 6000 were significantly inhibited in the early stage and then up-regulated. (nefu.edu.cn)
- Some humans have very low levels of catalase (acatalasia), yet show few ill effects. (wikipedia.org)
- In contrast, the levels of SOD, and catalase activities significantly decreased after treatment. (edu.sa)
- In addition, inoculation of two mycorrhizal fungi under cold stress significantly increased the relative expression levels of PtPOD and PtF-SOD in leaves, P. indica up-regulated the expression levels of PtCu/Zn-SOD in leaves, and D. versiformis also induced the expression levels of PtMn-SOD and PtCAT1 in leaves. (techscience.com)
Cloning2
- Cloning, expression, purification and ch. (agu.edu.tr)
- Cloning and Expression Analysis of Catalase Gene( LoCAT 1) from Larix olgensis [J]. Bulletin of Botanical Research, 2019, 39(4): 539-546. (nefu.edu.cn)
Modulation2
- To examine the morphological effects of catalase gene transfer in modulation of H 2 O 2 induced injury, transduced corneas were maintained in ex vivo culture and challenged with H 2 O 2 . (bmj.com)
- 2007). Modulation of Frankia alni ACN14a oxidative stress response: Activity, expression and phylogeny of catalases . (up.pt)
Tissues2
- However, "Copper deficiency can lead to a reduction in catalase activity in tissues, such as heart and liver. (wikipedia.org)
- The present study was performed to compare the expression of Foxo3a in periapical granulomas and healthy gingival tissues. (go.jp)
Genes1
- In order to know about genes of Larix olgensis catalase and determine the expression characteristics in different organs of L. olgensis under different stresses, a CAT 1 gene was cloned based on specific primers designed according to the CAT 1 gene sequences from transcriptome database of L. olgensis , named LoCAT 1. (nefu.edu.cn)
Reduction1
- In light-damaged retinas, 5 days of HRS treatment increased Sirt1 expression, mitigated a- and b-wave amplitude reduction, and decreased the reduction of outer nuclear cell layers. (arvojournals.org)
Characterization1
- Characterization of miRNA expression profile provided support for AR , PPAR, retinoic acid signaling and inflammation in miniaturized hair. (nature.com)
Concentration2
- The decomposition of hydrogen peroxide by catalase proceeds according to first-order kinetics, the rate being proportional to the hydrogen peroxide concentration. (wikipedia.org)
- Se alone or combined with VE increased the concentration of selenoprotein P and selenoproteins mRNA level and decreased the expression of HSP60 . (springeropen.com)
Endothelial cells1
- To examine the effect of catalase gene transfer on survival of corneal endothelial cells (EC) following challenge with hydrogen peroxide (H 2 O 2 ) in an ex vivo model of oxidative stress. (bmj.com)
Significant3
- However, lack of expression and insolubility remain significant challenges to the expression and crystallization of these proteins. (iucr.org)
- Significant upregulation of catalase (CAT) was observed only after RW. (whiterose.ac.uk)
- Foxo3a was expressed in 82.1%, 78.3%, and 77.5% of neutrophils, T lymphocytes, and B lymphocytes, respectively, and statistical analysis using the Kruskal-Wallis test followed by the Steel-Dwass test showed no significant difference of Foxo3a expression among the three cell types. (go.jp)
Activity1
- Catalase activity increased with supplementation in the YA and aged animals. (cdc.gov)
Recombinant2
Activities1
- APX activities and the relative expression of APX were also stimulated in grafted seedlings, although their changes were not completely consistent. (tubitak.gov.tr)
Tumor1
- Macrophage secretion of IL-1β was found to induce tumor expression of ICAM1. (nih.gov)
Species1
- The pH optimum for other catalases varies between 4 and 11 depending on the species. (wikipedia.org)
Human4
- The optimum pH for human catalase is approximately 7, and has a fairly broad maximum: the rate of reaction does not change appreciably between pH 6.8 and 7.5. (wikipedia.org)
- Human catalase forms a tetramer composed of four subunits, each of which can be conceptually divided into four domains. (wikipedia.org)
- In an anagen human hair follicle PPARγ expression is detected in the mesenchymal DP cells, epithelial cells of the outer root sheath (ORS), inner root sheath and matrix 16 . (nature.com)
- These in vitro, and their expression in these human tumour virus. (who.int)
Hydrogen5
- one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second. (wikipedia.org)
- Catalase was first noticed in 1818 by Louis Jacques Thénard, who discovered hydrogen peroxide (H2O2). (wikipedia.org)
- Furthermore, the poison cyanide is a noncompetitive inhibitor of catalase at high concentrations of hydrogen peroxide.Arsenate acts as an activator. (wikipedia.org)
- To this end, catalase is frequently used by cells to rapidly catalyze the decomposition of hydrogen peroxide into less-reactive gaseous oxygen and water molecules. (wikipedia.org)
- Catalase helps H pylori survive in the host by preventing the formation of reactive oxygen metabolites from hydrogen peroxide. (medscape.com)
Induces1
- Expression of vacuolating cytotoxin, which induces inflammatory cytokines, may be associated with more pronounced inflammation and increased propensity to cause disease. (medscape.com)
Liver3
- In 1937 catalase from beef liver was crystallized by James B. Sumner and Alexander Dounce and the molecular weight was measured in 1938. (wikipedia.org)
- However, catalase deficiency in mice may increase the likelihood of developing obesity, fatty liver, and type 2 diabetes. (wikipedia.org)
- In liver, the diet KEECC significantly increased catalase expression compared with diet 0. (stir.ac.uk)
Mice2
- Mice genetically engineered to lack catalase are initially phenotypically normal. (wikipedia.org)
- The increased oxidative stress that occurs with aging in mice is alleviated by over-expression of catalase. (wikipedia.org)
Vector1
- Cells co-transfected with an estrogen response element (ERE)-driven luciferase (Luc) reporter gene and an ERalpha- or ERbeta-expression vector were exposed to graded concentrations of ellagic acid. (nutrimedical.com)
Journal1
- Yu D L,Wang C L. Progresses in the research of catalase[J]. Chinese Journal of Histochemistry and Cytochemistry,2016,25(2):189-194. (nefu.edu.cn)
Tolerance1
- Enhanced drought and photooxidation tolerance of transgenic tobacco plants overexpressingpea catalase in chloroplasts[J]. Acta Ecologica Sinica,2011,31(4):1058-1063. (nefu.edu.cn)
Patterns1
- The expression patterns of LoCAT 1 were not exactly similarity among these treatments. (nefu.edu.cn)
Found1
- In 1900, Oscar Loew was the first to give it the name catalase, and found it in many plants and animals. (wikipedia.org)
Yield1
- Because of an inherent limitation (extremely low expression), Cys at position 34 of the 3r germ line was replaced by Tyr reaching a good expression yield. (osti.gov)
Highest1
- While expression level in leaves was significantly inhibited at 24 h, and then up-regulated, with the highest expression at 96 h. (nefu.edu.cn)