INOSInducible nitric oxide synthasIsoformsNos2Oxidative StressInhibitionIsoformEpithelial cellsNeuronalCytokinesPathwayEndothelial cellsHepatocytesProtein expressionNnosMacrophagesRegulationInductionReactiveVasodilatorGenesAbstractRats264.7VascularBasalProstaglandinMediatorsMiceIncreasesPatientsProteinsSuperoxide dismutaseLungInflammatoryMarkerRolesRoleVitroSuppressionENOS proteinProductionInduceFunctionTissueHepatic
INOS15
- In the current study, we show that delta 9-THC produces a marked inhibition of inducible nitric oxide synthase (iNOS) transcription and nitric oxide production by the macrophage line RAW 264.7 in response to lipopolysaccharide (LPS). (aspetjournals.org)
- Collectively, this series of experiments indicates that NF-kappa B/Rel is positively regulated by the cAMP cascade to help initiate iNOS gene expression in response to LPS stimulation of macrophages. (aspetjournals.org)
- Quantitative reverse transcription-polymerase chain reaction (RT-PCR) experiments revealed an induction of myometrial smooth muscle endothelial NOS (eNOS) expression by progesterone and 17beta-oestradiol, while myometrial iNOS expression was unaffected by steroid hormones. (ox.ac.uk)
- Moreover, protein expression of pro-inflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were investigated using Western blot analysis. (ac.ir)
- Saturated sesquiterpene lactones-rich species (Artemisia kopetdaghensis, Artemisia santolina, Artemisia sieberi) showed the highest suppressive activity on NO and PGE2 production via suppression of iNOS and COX-2 expression. (ac.ir)
- Nitric oxide (NO) can modulate numerous genes through several pathways, yet some genes may be modulated only in the presence of the inflammatory stimuli that upregulate the inducible nitric oxide synthase (iNOS) rather than by NO alone. (usuhs.edu)
- Furthermore, the role of prior expression of iNOS in the modulation of genes by NO is unknown. (usuhs.edu)
- Northern analysis performed with this fragment demonstrated that treatment of iNOS -/- hepatocytes with Ad-iNOS or with the NO donor Snitroso-N-acetyl-D,L-penicillamine (SNAP), but not control treatment or infection with Ad-LacZ, resulted in increased expression of CYP2E1. (usuhs.edu)
- In the present study, we investigate the effect of the bark methanol extract (BLM) on reactive oxygen/nitrogen species (ROS/RNS), the expression of protein and mRNA of inducible nitric oxide synthase (iNOS) in RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS) and sodium nitroprusside (SNP) to provide scientific validation of the above said medicinal property. (phcog.com)
- The protein and transcript expression of iNOS was evaluated through Western Blot and reverse transcription-polymerase chain reaction (RT-PCR) analysis respectively. (phcog.com)
- 100 μg/ml (1.6-fold) credited to a reduced protein and mRNA expression of iNOS as recorded by Western blots and RT-PCR results respectively. (phcog.com)
- 6 of the 23 patients had high levels of inducible nitric oxide synthase (iNOS), a source of microbicidal nitric oxide. (utmb.edu)
- The aim of the presented study was to perform the immunohistochemical detection of endothelial (eNOS) and inducible (iNOS) isoform of nitric oxide synthase in the adenomatous and hyperplastic parathyroid gland in relation to the apoptotic process. (nel.edu)
- However, these previous studies did not determine the relative contributions of COX (COX‐1 and COX‐2) and nitric oxide synthase (NOS) isoforms (endothelial constitutive (ecNOS) and inducible NOS (iNOS)) to the abnormal acinar microvessel response to HA 4 , 7 . (ersjournals.com)
- This treatment induced a significant increase in the levels of proinflammatory mediators including the inducible Nitric Oxide Synthase (iNOS), interleukin 1 Beta (IL-1β) and Interleukin-12 (IL-12). (lu.se)
Inducible nitric oxide synthas3
- Attenuation of inducible nitric oxide synthase gene expression by delta 9-tetrahydrocannabinol is mediated through the inhibition of nuclear factor- kappa B/Rel activation. (aspetjournals.org)
- The expression of the inducible nitric oxide synthase gene was evaluated with reverse transcription-PCR. (molvis.org)
- Subcutaneous injection of sodium selenite led to severe oxidative damage in the lenticular tissues, shown by increased formation of formazan crystals, elevated generation of superoxide, hydroxyl, and nitric oxide radicals, and elevated inducible nitric oxide synthase gene and protein expression that possibly contributed to the opacification of the lens and thus cataract formation. (molvis.org)
Isoforms4
- The aim of this study was to measure NO production in women with and without menorrhagia to detect nitric oxide synthase (NOS) isoforms in uterine cells and to investigate any steroid effects on myometrial NOS expression. (ox.ac.uk)
- NO production in the brain is catalyzed by three isoforms of NO synthase (NOS) including neuronal NOS (nNOS), inducible NOS and endothelial NOS. (johnshopkins.edu)
- The expression of NO synthase (NOS), cyclooxygenase (COX) isoforms, soluble guanylate cyclase, superoxide dismutase and the NAD(P)H oxidase subunits p22phox and p47phox were determined. (uab.cat)
- Constitutive, but not inducible, isoforms of cyclo-oxygenase and nitric oxide synthase play an important role in abnormal microvessel responses to carbon dioxide and hydrogen ions in hyperoxia-injured lungs. (ersjournals.com)
Nos23
- Nitric oxide synthase, inducible is an enzyme which is encoded by the NOS2 gene in humans and mice. (wikipedia.org)
- They lack the gene encoding nitric oxide synthase 2 (Nos2) and are susceptible to murine CMV infection. (wikipedia.org)
- We have analysed the mechanism by which palmitoylation permits the progression of nitric oxide synthase 2 (NOS2) along the ER-Golgi-TGN pathway. (biologists.com)
Oxidative Stress1
- Renal oxidative stress, mitochondrial morphology (electron microscopy), and NOX4 expression were assessed in 4-and 12-week-old PCK and Sprague-Dawley (wild-type, WT) control rats (n = 8 males and 8 females each). (tau.ac.il)
Inhibition3
- The chronic effects of PKCd inhibition using either rottlerin or the overexpression of a dominant negative PKCd mutant included the downregulation of eNOS gene expression that was manifested by a decrease in both eNOS promoter activity and protein expression after 24 h of treatment. (vectorbiolabs.com)
- The nitric oxide produced by ecNOS attenuated COX‐1 activity in injured arterioles and venules, but carbon dioxide enhanced it, leading to paradoxical dilatation of these microvessels under hypercapnic conditions with ecNOS inhibition. (ersjournals.com)
- Inhibition of inducible nitric oxide synthase with 1400W (1 mg/kg) abrogated the Cl2-induced changes in responsiveness. (cdc.gov)
Isoform1
- In this study, we explore the roles of the delta isoform of PKC (PKCd) in the regulation of endothelial nitric oxide synthase (eNOS) activity in pulmonary arterial endothelial cells isolated from fetal lambs (FPAECs). (vectorbiolabs.com)
Epithelial cells1
- Nitric oxide synthase is expressed in epithelial cells of the liver, lung and bone marrow. (wikipedia.org)
Neuronal2
- Neuronal nitric oxide synthase (nnos) expression following inferior alveolar nerve injury. (ox.ac.uk)
- Nitric oxide (NO) is a gas messenger with diverse physiological roles in the nervous system, from modulation of synaptic plasticity and neurogenesis to the mediation of neuronal death. (johnshopkins.edu)
Cytokines2
- The serum levels of proinflammatory cytokines as well as the expression of inflammation- and apoptosis-related genes in colonic tissue were also analyzed. (carcinogenesis.com)
- Administration of the doenjang using probiotic mixed starter cultures ameliorated the symptoms of colon cancer, and reduced the incidence of neoplasia, and reduced the levels of serum proinflammatory cytokines such as interleukin-6, and tumor necrosis factor-α and inducible nitric oxide synthase and cycloooxygenase-2 expression levels in colonic tissue. (carcinogenesis.com)
Pathway1
- Both genes affect the nitric oxide synthase pathway and are altered by hypoxia. (nih.gov)
Endothelial cells1
- Certain studies show that P. gingivalis lipopolysaccharide (LPS) could induce the expression of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 in human umbilical vein endothelial cells (HUVECs) ( 13 , 14 ), which significantly enhances trans-endothelial migration of inflammatory cells. (spandidos-publications.com)
Hepatocytes1
- Rat hepatocytes treated with SNAP also exhibited increased expression of CYP2E1 mRNA. (usuhs.edu)
Protein expression2
- These data suggest that the observed enhancement of eNOS protein expression could constitute a compensatory mechanism to counter-regulate a chronic loss of NO possibly through increased superoxide anion production from NAD(P)H oxidase induced by age. (uab.cat)
- When rat pups were treated with intraperitoneal administration of the extract of C. maritima , the generation of free radicals as well as the messenger ribonucleic acid and protein expression of inducible nitric oxide synthase were maintained at near normal levels. (molvis.org)
Nnos3
- Of the siRNA sequences screened, one corresponding to exon 10 of the rat nNOS specifically and efficiently inhibited nNOS expression at the mRNA and protein level. (johnshopkins.edu)
- To demonstrate the anatomical specificity of nNOS silencing in vivo, vectors were used to selectively knock-down the endogenous nNOS expression in cortical GABAergic interneurons of rat piriform cortex. (johnshopkins.edu)
- The ability of our nNOS silencing vectors to effectively and precisely silence nNOS expression shows their value as research tools for further studies of the role of nNOS in specific brain circuits. (johnshopkins.edu)
Macrophages2
- Nitric oxide level produced by LPS-primed macrophages was significantly decreased with all prepared fractions in a dose-dependent manner. (ac.ir)
- Treatment of murine macrophages (J774A.1) with Mitsui-7 MWCNTsincreased the expression of Alox5 mRNA and protein in a concentration- and time-dependent manner. (cdc.gov)
Regulation2
- Nitric oxide synthase expression and steroid regulation in the uterus of women with menorrhagia. (ox.ac.uk)
- It is possible that nitric oxide, which might play an important role in the regulation of cell proliferation, is a major target of the toxic effects of alcohol. (elsevierpure.com)
Induction2
- Inducible nitric oxide synthase is involved in the induction of changes in responsiveness to methacholine. (cdc.gov)
- The MWCNTs induced the expression of CD68 and that induction persisted for up to 3days post-exposure. (cdc.gov)
Reactive2
- citation needed] Nitric oxide is a reactive free radical mediating in neurotransmission, antimicrobial and antitumoral activities. (wikipedia.org)
- There is an overwhelming mass of evidence demonstrating the development of endothelial dysfunction in animal models of diabetes and in human blood vessels from diabetic patients, as evidenced by increased release of reactive oxygen species, decreased nitric oxide (NO) bioactivity, decreased release of prostacyclin (PGI 2 ), and enhanced endothelial production of vasoconstrictor thromboxane (Tx)A 2 /prostaglandin (PG)H 2 in early stages of diabetes ( 1 - 3 ). (diabetesjournals.org)
Vasodilator1
- The free-radical and vasodilator nitric oxide (NO) relaxes the myometrial smooth muscle and is a strong candidate for the cause of excessive blood loss in menorrhagic patients. (ox.ac.uk)
Genes6
- We used RNA sequencing coupled with chromatin immunoprecipitation sequencing to analyze primed microglial inflammatory gene expression and modifications to histone H3 lysine 4 trimethylation (H3K4me3) at the promoters of primed genes. (frontiersin.org)
- After 3 weeks, we compared body weight, food intake, plasma levels of lipids and glucose, and the expression patterns of the clock genes and the genes involved in lipid metabolism in the liver and WAT. (researchgate.net)
- LL eliminated the circadian rhythms of the expression of the clock genes as well as most of the genes involved in lipid metabolism in both liver and WAT. (researchgate.net)
- More notably, LL markedly decreased not only the amplitude but also the average levels of the expression of the genes in the liver, but not in the WAT, suggesting that transcription in the liver is sensitive to constant light exposure. (researchgate.net)
- Here, we assessed the combined effect of diet composition and feeding time on (1) body composition, (2) energy balance, and (3) circadian expression of hepatic clock and metabolic genes. (researchgate.net)
- These findings indicate that the doenjang attenuated colon carcinogenesis induced by AOM and DSS by ameliorating the symptoms of colon cancer, reducing the occurrence of neoplasia, regulating proinflammatory cytokine levels, and controlling the expressions of inflammation- and apoptosis-related genes in the colonic tissue. (carcinogenesis.com)
Abstract1
- abstract = "Our aim was to study the role of nitric oxide (NO) and arachidonic acid pathways in the α1-adrenoceptor-mediated vasoconstriction in mesenteric resistance arteries from 3-4 and 22 to 23-month-old Sprague-Dawley rats. (uab.cat)
Rats3
- In this study, effects of Puerariae radix on cell proliferation and nitric oxide synthase expression in the dentate gyrus of alcohol-intoxicated Sprague-Dawley rats were investigated via 5-bromo-2′-deoxyuridine (BrdU) immunohistochemistry and nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry. (elsevierpure.com)
- Renal expression of NOX4 was upregulated in TECs and ECs of PCK rats at 4 weeks of age and further increased at 12 weeks. (tau.ac.il)
- Vitamin D inhibits proliferation and profibrotic marker expression in hepatic stellate cells and decreases thioacetamide-induced liver fibrosis in rats. (springer.com)
264.71
- Treatment of RAW 264.7 with delta 9-THC inhibited forskolin-stimulated cAMP production in a dose-related manner, verifying the expression of functional cannabinoid receptors by this cell line. (aspetjournals.org)
Vascular1
- The levels of vascular endothelial (VE)‑cadherin, endothelial nitric oxide synthase (eNOS) and cysteinyl aspartate‑specific protease‑3 (caspase‑3) were measured using western blot analysis. (spandidos-publications.com)
Basal1
- We, therefore, tested the hypothesis that hypoxia would enhance or "prime" microglial pro-inflammatory gene expression in response to a later inflammatory challenge without programmatically increasing basal levels of pro-inflammatory cytokine expression. (frontiersin.org)
Prostaglandin1
- In addition, nitric oxide (NO) release was measured using Griess method and prostaglandin E2 (PGE2) level was determined by enzyme-linked immunosorbent assay (ELISA). (ac.ir)
Mediators1
- In this study, quantitative real-time polymerase chain reaction was used to determine the levels of expression of inflammatory and immune mediators in skin-biopsy samples collected from 23 untreated patients with Mediterranean spotted fever (MSF). (utmb.edu)
Mice1
- Stabilization Restores Epigenetic Control of Nitric Oxide Synthase 1 Expression and Reverses Gastroparesis in Female Diabetic Mice. (bvsalud.org)
Increases1
- Beta glucan increases serum nitric oxide in hypercholesteremic patients. (greenmedinfo.com)
Patients1
- The mRNA-expression level of the chemokine RANTES was significantly higher in patients with severe MSF. (utmb.edu)
Proteins2
- Increased inducible nitric oxide synthase expression and oxidation of lung proteins were observed. (cdc.gov)
- P. gingivalis HSP60 at a concentration of 10 ng/l significantly decreased the expression levels of VE‑cadherin and eNOS protein at 24 h stimulation, whereas no difference in these proteins was identified following a low dose of P. gingivalis HSP60 (1 ng/l). (spandidos-publications.com)
Superoxide dismutase1
- Ageing up-regulated endothelial NOS and p22phox expression but did not modify COX, soluble guanylate cyclase, superoxide dismutase and p47phox expression. (uab.cat)
Lung1
- In a previous study, it was demonstrated that HA induces distinct venule dilatation closely associated with vasodilating prostaglandins generated by cyclo-oxygenase (COX), but not by nitric oxide (NO), in intact rat lung 4 . (ersjournals.com)
Inflammatory1
- Mild/moderate MSF is associated with a strong and balanced intralesional proinflammatory and anti-inflammatory response, with a dominant type 1 immunity, whereas severe MSF is associated with increased expression of chemokine mRNA. (utmb.edu)
Marker1
- The expression and activity of inducible nitric oxide synthase, an intracellular marker of M1, were increased by MWCNTs. (cdc.gov)
Roles1
- In the present study, we aimed to study the roles of endothelial nitric oxide synthase (eNOS) in diabetes. (diabetesjournals.org)
Role1
- The aim of the present study was to compare microvessel responses to hypercapnic and isocapnic acidosis in hyperoxia-injured lungs and to assess the role of constitutive and inducible forms of nitric oxide synthase (NOS) and cyclo-oxygenase (COX). (ersjournals.com)
Vitro1
- These in vitro, and their expression in these human tumour virus. (who.int)
Suppression1
- Lundquist I, Mohammed Al-Amily I, Meidute Abaraviciene S, Salehi A. Metformin Ameliorates Dysfunctional Traits of Glibenclamide- and Glucose-Induced Insulin Secretion by Suppression of Imposed Overactivity of the Islet Nitric Oxide Synthase-NO System. (lu.se)
ENOS protein1
- P. gingivalis HSP60 at 100 ng/l significantly downregulated the expression levels of VE‑cadherin and eNOS protein at 12 h in HUVECs. (spandidos-publications.com)
Production2
- Fractions bearing unusual (Artemisia fragrans and Artemisia absinthium) and unsaturated sesquiterpene lactones (Artemisia ciniformis) possess less modulatory effect on PGE2 production and COX-2 expression. (ac.ir)
- A combination of polyphenols reduced diastolic pressure by potentiating eNOS activation and nitric oxide production. (greenmedinfo.com)
Induce2
- Objective Eating out of phase with the endogenous biological clock alters clock and metabolic gene expression in rodents and can induce obesity and type 2 diabetes mellitus. (researchgate.net)
- These results indicated that P. gingivalis HSP60 may induce dysfunction and apoptosis in HUVECs via downregulating the expression levels of VE‑cadherin and eNOS, and promoting the cleavage of caspase‑3. (spandidos-publications.com)
Function2
- The only loss-of-function variant was a homozygous frameshift mutation in nitric oxide synthase 2. (wikipedia.org)
- Endothelial function was assessed by renal expression of endothelial nitric oxide synthase (eNOS). (tau.ac.il)
Tissue1
- Relationship between inducible nitric oxide synthase expression and angiogenesis in primary gallbladder carcinoma tissue. (wjgnet.com)
Hepatic1
- Vitamin D ameliorates stress ligand expression elicited by free fatty acids in the hepatic stellate cell line LX-2. (springer.com)