• Located in cytosol and plasma membrane. (nih.gov)
  • Moreover, we identified two structural motifs in transmembrane segments 2 and 4 involved in mTRPA1-cholesterol interactions that are necessary for normal agonist sensitivity and plasma membrane localization. (elifesciences.org)
  • However, it is currently unknown whether TRPA1 is localized in specific domains of the plasma membrane, or whether cholesterol interacts with specific residues of TRPA1. (elifesciences.org)
  • HvPIP1;6, a Barley (Hordeum vulgare L.) Plasma Membrane Water Channel Particularly Expressed in Growing Compared with Non-Growing Leaf Tissues. (lu.se)
  • They can be membrane-bound receptors such as GPCRs , ion channels , transporters , enzymes, or others. (calixar.com)
  • Starting from native material or recombinant systems, we succeed with all types of membrane proteins: GPCRs, Ion Channels, Transporters, Receptors and Viral Proteins. (calixar.com)
  • Expression levels of the primary neuronal glucose transporters (GLUTs) isoforms 1, 3, 4, and 8 were found to be unaltered within a 24 h period after excitation. (jneurosci.org)
  • Using cellular disease models for ADTKD-SEC61A1, we identified an impaired protein transport of the renal secretory protein renin and a reduced abundance of regulatory calcium transporters, including SERCA2. (amrita.edu)
  • However, the limited set of chromatophore-encoded solute transporters appears insufficient for supporting metabolic connectivity or protein import. (frontiersin.org)
  • Here we show by mass spectrometric analyses of enriched insoluble protein fractions that, unexpectedly, nucleus-encoded transporters are not inserted into the chromatophore inner envelope membrane. (frontiersin.org)
  • Thus, despite the apparent maintenance of its barrier function, canonical metabolite transporters are missing in this membrane. (frontiersin.org)
  • Very lipophilic drugs enter the cell membrane through passive diffusion, but polar or ionized organic compounds can enter the cell membrane only by transporters. (helsinki.fi)
  • Transporters in the basolateral membrane of the hepatocyte facilitate drug entry and access to drug metabolizing enzymes. (helsinki.fi)
  • These studies involve examination of membrane lipid and transporters as well as solute changes. (uoguelph.ca)
  • CALIXAR's approach allows to preserve the original structure and function of membrane proteins (GPCRs, Ion Channels, Transporters, Receptors, Anchors and Viral Proteins) providing solutions for pharmaceutical industries, biotechnology companies and academic teams to develop conformational antibodies, formulate new vaccines, carry out Structure Based Drug Discovery and/or HTS assays. (calixar.com)
  • The lectures address the three different main types of membrane proteins and associated cellular processes: transport and transporters, signal transduction and receptors, bioenergetics and photosynthetic and respiratory proteins. (lu.se)
  • Among the different families of membrane proteins, the so-called G protein-coupled receptors (GPCRs) comprise the largest family. (mpg.de)
  • Our structural and functional assays demonstrate the different binding modes of chemically distinct agonists of S1PRs, reveal the mechanical switch that activates these receptors, and provide a framework for understanding ligand selectivity and G protein coupling. (rcsb.org)
  • Both receptors and ligands are membrane-bound proteins that require direct cell-cell interaction for activation. (hindawi.com)
  • We are primarily using cytomegaloviruses to examine how the pathogens alter signaling pathways directed by G-protein coupled receptors (GPCRs) to facilitate robust replication in tissues important for host-host dissemination. (uc.edu)
  • Among the bacterial systems available, the Gram-positive lactic bacterium, Lactococcus lactis , traditionally used in food fermentations, is nowadays widely used for large-scale production and functional characterization of bacterial and eukaryotic membrane proteins. (springer.com)
  • The aim of this chapter is to describe the different possibilities for the functional characterization of peripheral or intrinsic membrane proteins expressed in Lactococcus lactis . (springer.com)
  • CALIXAR's membrane protein services include membrane protein expression , solubilization, purification & characterization among others. (calixar.com)
  • Molecular characterization and expression of heparan-sulfate 6-sulfotransferase. (nih.gov)
  • Purification and characterization of two protein kinases acting on the aquaporin SoPIP2;1. (lu.se)
  • Saturation of the cellâ s protein folding capacity and accumulation of inactive incompletely folded protein often accompanying the overexpression of membrane proteins (MPs) presents an obstacle to their efficient purification in a functional form for structural studies. (westminster.ac.uk)
  • Purification and Proteomic Analysis of Plant Plasma Membranes. (lu.se)
  • Lectures dealing with methods for theoretical modelling of membrane protein structure, fusion protein techniques, X-ray crystallography, heterologous expression, solubilisation and purification of membrane proteins are also included in the course. (lu.se)
  • In silico exercise addressing potential problems concerning the detection of heterologously expressed membrane proteins, solubilisation and evaluation of detergent properties, ion exchange chromatography and gel filtering in the presence of a detergent, and control of the protein's stability and integrity after purification. (lu.se)
  • As a solution NEB offers a tunable T7 expression strain for the production of membrane proteins and other difficult targets so that the level of functional protein may be optimized. (neb.com)
  • HomB protein contributes to the colonization and persistence of H. pylori , and the presence of homB genes affects the number of bacteria adhering to the host cells. (nature.com)
  • It is estimated that one-third of the genes of an organism encode integral membrane proteins (1). (mpg.de)
  • When such a signal changes expression of genes coding for INM or a chromatin-modifying enzymes, it can induce differentiation in to a different cell type. (wikipedia.org)
  • Our project offers a unique opportunity for Master's student to gain hands-on experience focused on transcriptional regulators with importance in expression of genes of outer membrane proteins that are essential in host-pathogen interaction. (lu.se)
  • The most common cause of HCM is now known to be one of over 200 possible mutations in at least 10 genes that involve sarcomeric proteins. (medscape.com)
  • Supervised analysis of kinase genes revealed a high-level FLT3 expression in a subset of cases without molecular rearrangements. (aacrjournals.org)
  • Total RNA from tumors the expression of thousands of genes (3) to address complex questions was isolated using two successive rounds of Trizol. (lu.se)
  • In AMB-exposed biofilms, 2.7% of the genes showed altered expression, while in CAS-exposed biofilms, 13.0% of the genes had their expression modified. (who.int)
  • To determine TRPA1 localization in cellular membranes we stained HEK293T cells transfected with the mTRPA1-mCherry construct with the Vybrant Alexa Fluor 488 Lipid Raft Labeling Kit. (elifesciences.org)
  • We have investigated the developmental expression and subcellular localization of synapsin III, the newest member of the synapsin family, in cultured mouse hippocampal neurons. (jneurosci.org)
  • Validation of the localization of targets (a type of membranes). (calixar.com)
  • Drug discovery stages: target identification and validation, deorphanization, membrane protein localization, studies about the mechanism of action of specific targets/ligands or targets/hits. (calixar.com)
  • H. pylori attaches and colonizes to the human epithelium using some of their outer membrane proteins (OMPs). (nature.com)
  • Outer membrane proteins (OMPs) of H. pylori play a crucial role in the host-pathogen interaction, virulence and pathogenesis. (nature.com)
  • Hom (Helicobacter outer membrane) family of OMPs in H. pylori consists of four members (HomA, B, C and D). In the H. pylori genome jhp 0870 open-reading frame (ORF) that codes for HomB outer membrane protein is associated with many stomach diseases and is a novel co-marker for peptic ulcer disease (PUD) 9 , 10 . (nature.com)
  • Outer membrane proteins (OMPs) are distinctive features of Gram-negative bacteria. (nature.com)
  • in the cytoplasm, the cytoplasmic ER, or the outer nuclear membrane. (wikipedia.org)
  • The cagA and some outer membrane protein. (deu.edu.tr)
  • We then profiled their outer membrane proteins related to membrane permeability to drugs. (elsevierpure.com)
  • Neisseria meningitidis employs polysaccharides and outer membrane proteins to cope with human serum complement attack. (uni-wuerzburg.de)
  • Investigating the role of cell membrane proteins has increased over the last decade, as drugdrug interactions and genetic polymorphisms have been found to cause changes in drug pharmacokinetics and dynamics. (helsinki.fi)
  • All these structural determinations were performed on membrane proteins that are naturally highly expressed and can be purified in large quantities from their natural sources. (mpg.de)
  • ER protein determinations were performed using two-thirds of all breast cancers are ER at the time of diagnosis, the standard methods in the routine clinical laboratory (9). (lu.se)
  • There will be involved gene engineering and molecular biology methods such as PCR, SDS-PAGE, qPCR, western blot, DNA-protein interaction assays, flow cytometry etc. (lu.se)
  • CALIXAR's ligand binding assays services provide key information on ligand interactions with any membrane protein targets. (calixar.com)
  • CALIXAR's Thermal Stability Assays provides information on expression levels, stability and ligand binding of membrane proteins. (calixar.com)
  • The protocols describe the essential steps for obtaining diffraction quality crystals of a membrane protein starting from reconstitution of the protein in a lipidic cubic phase (LCP), finding initial conditions with LCP-FRAP pre-crystallization assays, setting up LCP crystallization trials and harvesting crystals. (jove.com)
  • Recent years have seen strong research efforts on the lipid component of biological membranes. (lu.se)
  • Here, we address this challenge by establishing a bacterial cell-free protein synthesis platform that enables rapid production of a variety of OSTs in their active conformations. (biorxiv.org)
  • This is followed by experimental determination using genetic construction and expression of a fusion protein of the membrane protein and a marker protein in a bacterial system which is subsequently analysed. (lu.se)
  • This new approach has been shown to preserve mobility and enzymatic activity of transmembrane proteins in the resulting nSLB. (lu.se)
  • The lack of effective method for preparative-scale membrane protein synthesis hampers progress toward a complete understanding of the proteome and prevents the exploitation of available sequences, especially those membrane proteins which constitute the majority of drug targets. (sbir.gov)
  • 2022 ) Protein domain-dependent vesiculation of Lipoprotein A, a protein that is important in cell wall synthesis and fitness of the human respiratory pathogen Haemophilus influenzae . (lu.se)
  • Facilities include those for protein-sequence analysis, peptide and oligonucleotide synthesis, access to the synchrotron at Lawrence Berkeley National Lab for X-ray crystallography, and NMR spectrometry. (berkeley.edu)
  • Following me will be Corinna Tuckey, who will describe the utility and advantages of the PURExpress in vitro protein synthesis kit. (neb.com)
  • Membrane proteins in translational control: characterisation of membrane-bound protein complexes associated with eIF2B, which regulates protein synthesis, and is important in neurological disease. (shu.ac.uk)
  • A particular challenge is the synthesis of oligosaccharyltransferases (OSTs), which catalyze the attachment of glycans to specific amino acid residues in target proteins. (biorxiv.org)
  • We recently developed a generic method for producing polymer-supported lipid bilayers directly from cell-derived native membrane vesicles (NMVs). (lu.se)
  • Molecular Membrane Biology 2013, 30 (3):246-260. (lu.se)
  • NEB also offers solutions for expressing these types of proteins, either in vitro or in vivo. (neb.com)
  • In this study the characteristics of the OATP1B1 transporter were reviewed and new in vitro research method to study protein functions was developed. (helsinki.fi)
  • The purpose of this study was to demonstrate experimentally that alterations of in vivo transporter function at the blood-brain barrier (BBB) in disease and during pharmacotherapy can be reconstructed from in vitro data based on our established pharmacoproteomic concept of reconstructing in vivo function by integrating intrinsic transport activity per transporter molecule and absolute protein expression level at the BBB. (aspetjournals.org)
  • Aerosolized conidia and fungal fragments were collected onto protein binding membranes direct from sporulating in vitro culture plates. (cdc.gov)
  • These in vitro, and their expression in these human tumour virus. (who.int)
  • Transmembrane protein 88 inhibits transforming growth factor-β1-induced-extracellular matrix accumulation and epithelial-mesenchymal transition program in human pleural mesothelial cells through modulating TGF-β1/Smad pathway. (nih.gov)
  • The aim of this study was to examine whether Annexin A2 and S100A10 expression can be used as prognostic markers for epithelial ovarian cancer (EOC). (cancerindex.org)
  • CFTR is a cyclic adenosine monophosphate (cAMP)-regulated chloride channel, regulating chloride, sodium, and bicarbonate transport across epithelial membranes. (msdmanuals.com)
  • AQP5-L51P increased vimentin expression of in human lens epithelial cells. (bvsalud.org)
  • Quantitative targeted absolute proteomic analysis of 31 membrane proteins showed that P-glycoprotein (P-gp/mdr1a) protein expression levels were significantly increased in brain capillaries of PTZ (129%), EL (143%), and PHT mice (192%) compared with controls. (aspetjournals.org)
  • Further, biophysical experiments suggested that HomA and HomB are dimeric and most likely the cysteine residues present on surface-exposed loops participate in protein-protein interactions. (nature.com)
  • Once INM:chromatin interactions have been established following formation of the nuclear envelope, soluble nuclear proteins may bind to exposed chromosomal segments. (wikipedia.org)
  • We maintain protein-protein and protein-ligand interactions in a native environment. (calixar.com)
  • We discuss the impact of such interactions on TRPA1 gating mechanisms, regulation by the lipid environment, and role of this channel in sensory membrane microdomains, all of which helps to understand the puzzling pharmacology and pathophysiology of this channel. (elifesciences.org)
  • We are able to Maintain protein-protein and protein-ligand interactions in native environments. (calixar.com)
  • We can control protein-protein interactions in the native environment and thus enable our clients to generate highly relevant and reliable information about membrane proteins. (calixar.com)
  • I am committed to interdisciplinary research, and an example of the successes gained from this approach is the project with Prof A Nabok (Engineering Sheffield Hallam University) using total internal reflection ellipsometry to quantify protein-membrane interactions on native plant membranes and human cell lines. (brookes.ac.uk)
  • Program access grant to the STFC Harwell Laser Facility 'The Plant Cell Initiative: Protein interactions in the higher plant secretory pathway' 2017-2021 (approximate value of £200K). (brookes.ac.uk)
  • Monné M, Chan KW, Slotboom DJ, Kunji ERS (2005) Functional expression of eukaryotic membrane proteins in Lactococcus lactis. (springer.com)
  • These data indicate that this eukaryotic cell-free expression system has a high success rate and that the addition of specific reagents can increase the yield of soluble protein. (iucr.org)
  • We characterized a glucose dependent hyperpolarization of the mitochondrial membrane potential (Δψ m ) in the majority of neurons after transient glutamate excitation. (jneurosci.org)
  • 2010). Cytochrome c oxidase is regulated by modulations in protein expression and mitochondrial membrane phospholipid composition in estivating African lungfish. (uoguelph.ca)
  • and, regulation of biological processes such as chromosomal folding, protein secretion and intracellular signaling. (berkeley.edu)
  • The membrane and metabolic alterations during metabolic arrest in terrestrial snails and African lungfish are also of interest since these organisms undergo a reduction in metabolism at a constant temperature (estivation). (uoguelph.ca)
  • Membranes and metabolism of fishes. (uoguelph.ca)
  • Membrane proteins play a key role in the cell's energy metabolism and in its signalling and communication with its environment. (lu.se)
  • Research in the field of membrane proteins has undergone explosive growth during the last decade, primarily owing to the influence of the powerful techniques of modern molecular biology. (mpg.de)
  • With this Dynamics workshop, our goal is to bring together leading experts in the field of membrane dynamics, with a particular focus on neutrons and X-rays, but also complementary techniques including NMR, single molecule spectroscopy and computer modeling. (lu.se)
  • The main aim of the course is to enable students to acquire specialised knowledge and understanding of membrane biochemistry and the molecular structure, topology and functional mechanisms of membrane proteins. (lu.se)
  • Forms of HCM without evidence of mutations in sarcomeric proteins have also been identified. (medscape.com)
  • Despite their ubiquitous expression, the idiopathic SEC61A1 missense mutations p.V67G and p.T185A trigger a localized disease pattern diagnosed as autosomal dominant tubulointerstitial kidney disease (ADTKD-SEC61A1). (amrita.edu)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • In a few cases, sialin is produced but not routed properly to the lysosomal membrane. (medlineplus.gov)
  • Lysosomal-associated membrane protein-2 (LAMP2) is a heavily glycosylated protein found inside the lysosomal membrane. (medscape.com)
  • The nuclear scaffold is predicted to be relatively permanent for a given cell type, but induction of a signaling pathway-by ligand binding, cell:cell contact, or some other mechanism-can temporarily shift the expression profile. (wikipedia.org)
  • The screening used a genetically modified sequence type (ST)-41/44 clonal complex (cc) strain lacking LPS sialylation, polysaccharide capsule, the factor H binding protein (fHbp) and MutS, a protein of the DNA repair mechanism. (uni-wuerzburg.de)
  • Nearly all of the resonances in the two-dimensional 1H/15N HSQC spectrum of uniformly 15N labeled Vpu(2-30+) in micelles are superimposable on those from the corresponding residues in the spectrum of full-length Vpu, which indicates that the structure of the trans-membrane domain is not strongly affected by the presence of the cytoplasmic domain at its C terminus. (rcsb.org)
  • The analysis of the PISA wheels and Dipolar Waves obtained from both weakly and completely aligned samples show that Vpu(2-30+) has a trans-membrane alpha-helix spanning residues 8-25 with an average tilt of 13 degrees. (rcsb.org)
  • These diseases are a result of storage in cellular vacuoles and include Danon disease, Pompe disease, Fabry disease , and a form of HCM related to a mutation in the adenosine monophosphate (AMP)-activated, gamma-2 noncatalytic subunit of protein kinase ( PRKG2 ). (medscape.com)
  • Mounting evidence suggests that the genetic disorders/mutation and diseases change not only the protein expression patterns but also membranes themselves. (lu.se)
  • Glutamate excitation induced a rapid alteration in the AMP:ATP ratio that was associated with the activation of the AMP-activated protein kinase (AMPK). (jneurosci.org)
  • Notably, inhibition of the CaMKK (calmodulin-dependent protein kinase kinase) had little affect on GLUT translocation, whereas the inhibition or knockdown of AMPK (compound C, siRNA) activity prevented GLUT3 translocation to the cell surface after glutamate excitation. (jneurosci.org)
  • Among the few well-characterized INM proteins are lamin B receptor (LBR), lamina-associated polypeptide 1 (LAP1), lamina-associated polypeptide-2 (LAP2), emerin and MAN1. (wikipedia.org)
  • EPHA1, EPHA2, EFNA3, and EFNA4 mRNA expression was significantly higher in human epidermal growth factor receptor 2- (HER2-) positive tumors than in HER2-negative tumors. (hindawi.com)
  • Human cytomegalovirus G protein-coupled receptor US28 promotes latency by attenuating c-fos. (uc.edu)
  • BT-474 cells, obtained expression of the receptor has important implications for their biology from American Type Culture Collection, were maintained in RPMI 1640 and therapy (1). (lu.se)
  • Kunji ERS, Slotboom DJ, Poolman B (2003) Lactococcus lactis as host for overproduction of functional membrane proteins. (springer.com)
  • We present a novel strategy for optimization of functional MP expression in Saccharomyces cerevisiae. (westminster.ac.uk)
  • We demonstrate that a high level of UPR induction upon expression of a MP reflects impaired functional expression of that protein. (westminster.ac.uk)
  • Tuning the expression level of the protein so as to avoid or minimize UPR induction results in its increased functional expression. (westminster.ac.uk)
  • CALIXAR develops and implements full-length membrane proteins while maintaining their structural and functional integrity. (calixar.com)
  • Herein is described procedures implemented in the Caffrey Membrane Structural and Functional Biology Group to harvest and cryo-cool membrane protein crystals grown in lipidic cubic and sponge phases for use in structure determination using macromolecular X-ray crystallography. (jove.com)
  • When expressing membrane proteins, researchers must assume from the start that the protein will be difficult to express in functional form. (neb.com)
  • A number of proteins from each process, for which the structure is known, are explored in greater detail in order to highlight the functional molecular mechanisms. (lu.se)
  • There is extensive literature on the variables leading to insoluble recombinant expression of proteins. (iucr.org)
  • Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. (lu.se)
  • CALIXAR's native membrane protein expression service increases the success of producing reliable targets or antigens by adapting the expression conditions for each specific target. (calixar.com)
  • CALIXAR's membrane protein isolation service extracts native, wild-type, unmutated and untruncated targets so that pharmaceutical companies can produce antibodies and/or discovering primary leads. (calixar.com)
  • CALIXAR's Membrane Protein Identification services enable pharmaceutical corporations to utilize high-quality and stable targets at all discovery or clinical stages. (calixar.com)
  • You can leverage CALIXAR's exclusive technology as well as other best-in-class membrane protein technology not accessible anyplace else on the market today. (calixar.com)
  • 78573S The majority of membrane proteins are very difficult to obtain in significant quantities, even at milligram scale, because their natural biosynthesis levels often are very low and currently available protein expression systems are not effective for membrane proteins. (sbir.gov)
  • My additional independent research on ER localisation and splicing in auxin biosynthesis showed for the first time ER-localisation for an auxin biosynthetic protein. (brookes.ac.uk)
  • Furthermore, chromatophore-localized biosynthetic pathways as well as multiprotein complexes include proteins of dual genetic origin, suggesting that mechanisms evolved that coordinate gene expression levels between chromatophore and nucleus. (frontiersin.org)
  • Importantly, we highlight the limited correlation between transcriptomics and proteomics, emphasizing the critical role of membrane protein enrichment strategies and quantitative mass spectrometry. (lu.se)
  • Since many of today's expression vectors encode Shine-Dalgarno sequences it is easy to overlook problems with translation initiation. (neb.com)
  • Affinity tags can reduce merohedral twinning of membrane protein crystals. (lu.se)
  • Antigen expression was detected using mAb 9B4 in the IDA. (cdc.gov)
  • As proof-of-concept, the established target antigen CD44 was identified as a commonly and abundantly expressed surface protein with high endocytic activity. (lu.se)
  • We analyzed the developmental expression and subcellular distribution of synapsin III in cultured mouse hippocampal neurons and its role in neurite elongation and synapse formation. (jneurosci.org)
  • Here I pursued my scientific interests in subcellular protein localisation, bioinformatics, and mathematical modelling. (brookes.ac.uk)
  • Pertussis toxin B-pentamer mediates intercellular transfer of membrane proteins and lipids. (uc.edu)
  • Native membrane derived polymer-supported lipid bilayers (nSLBs) are poised to bridge the gap between live cell experiments and traditional model membrane architectures that by offering a combination of accessibility by surface sensitive analytical instrumentation and a composition which more closely resembles cellular membranes by displaying a diversity of endogenous membrane proteins, lipids, and carbohydrates. (lu.se)
  • Due to the lack of detergent solubilization and reconstitution steps, the nSLBs created using this approach contain essentially all of the native lipids, as well as the membrane-associated proteins and carbohydrates from the donor membrane. (lu.se)
  • This project will develop an in vivo membrane protein expression system for synthesizing any desired protein on a preparative scale, providing recombinant membrane proteins at quantities (grams) comparable to current soluble protein expression systems. (sbir.gov)
  • We anticipate that this broadly applicable production method will advance glycoengineering efforts by enabling preparative expression of membrane-embedded OSTs from all kingdoms of life. (biorxiv.org)
  • and protein stabilization. (nih.gov)
  • In our proposed project, you would investigate expression regulation in clinically significant bacterium non-typeable Haemophilus influenzae (NTHi). (lu.se)
  • Elucidate the structure, dynamics and functions of intrinsically disordered proteins and protein regions (IDPs/IDRs) and their biological regulation by Post-translational modifications. (upstate.edu)
  • In summary, our data suggest that the activation of AMPK and its regulation of cell surface GLUT3 expression is critical in mediating neuronal tolerance to excitotoxicity. (jneurosci.org)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
  • However, renal expression of the sodium/phosphate cotransporter gene, NPT2, is not required for regulation of renal 1 alpha-hydroxylase by phosphate. (thermofisher.com)
  • We employed a comprehensive approach for global and dynamic profiling of the surfaceome and endocytosed (endocytome) proteins and their regulation by hypoxia in patient-derived GBM cultures. (lu.se)
  • We provide a quantitative ranking of more than 600 surface resident and endocytosed proteins, and their regulation by hypoxia, serving as a resource to the cancer research community. (lu.se)
  • AQP5 expression, which is regulated by oxytocin, showed a dramatic down-regulation at the term and preterm uterus. (bvsalud.org)
  • Furthermore, we demonstrate the ability of cell-free synthesized OSTs to glycosylate multiple target proteins with varying N -glycosylation acceptor sequons. (biorxiv.org)
  • Understanding the mechanisms underlying protein folding and trafficking may contribute to the large-scale, therapy-based production of target proteins. (bioquicknews.com)
  • With the use of total internal reflection fluorescence (TIRF) microscopy we found a population of highly mobile vesicular structures near the cellular membrane containing both mTRPA1-mCherry and the lipid raft marker cholera toxin B ( Figure 1A and B , Video 1 ), as well as static areas where both fluorescent probes were colocalized at the membrane. (elifesciences.org)
  • Specifically, by using lipid nanodiscs as cellular membrane mimics, we obtained yields of up to 440 µg/mL for the single-subunit OST enzyme, 'Protein glycosylation B' (PglB) from Campylobacter jejuni , as well as for three additional PglB homologs from Campylobacter coli, Campylobacter lari , and Desulfovibrio gigas . (biorxiv.org)
  • Membrane proteins are one of the most challenging drug targets to produce and purify, because of their strong bond with the lipid bilayer, involving electrostaticity, hydrogen, and other types of binding. (calixar.com)
  • Membrane proteins play key roles in life processes, which is why they are attractive targets for drug discovery projects . (calixar.com)
  • Testing indicates that the system is a valuable tool for these protein targets. (iucr.org)
  • Our findings systematically elucidate the expression pattern and prognostic value of the EPHA/EFNA family in BrCa, which might provide potential prognostic factors and novel targets in BrCa patients, including those with different subtypes or treated with chemotherapy. (hindawi.com)
  • Expression of LMP1 in P3HR1 cells upregulates miR-146a levels. (nih.gov)
  • It has been proposed that chromatin-binding/modifying proteins embedded within the inner nuclear membrane may be central in determining the identity of newly differentiated cells. (wikipedia.org)
  • The structural features of the trans-membrane domain are determinants of the ion-channel activity that may be associated with the protein's role in facilitating the budding of new virus particles from infected cells. (rcsb.org)
  • To characterize gene expression signatures in acute lymphocytic leukemia (ALL) cells associated with known genotypic abnormalities in adult patients. (aacrjournals.org)
  • Maintenance of the expression plasmid should always be checked by plating cells at the point of induction on drug selection plates versus non-drug plates. (neb.com)
  • A further six years of postdoctoral research at the University of Manchester allowed me to advance my research skills and to tackle fundamental questions about how proteins are organised within cells. (shu.ac.uk)
  • Our common theme is investigating how proteins and RNA are delivered to their correct destination within cells, a universal process that can result in disease when it malfunctions. (shu.ac.uk)
  • The wild-type SLCO1B1-gene encoding the OATP1B1 was virulent with baculovirus into the cells by the Bac-to-Bac® Baculovirus Expression System. (helsinki.fi)
  • For expression in the Sf9 cells, the aim of the study was to clone the SLCO1B1-gene into the pFastBac vector. (helsinki.fi)
  • The expression of OATP1B1 transporter in HEK293 cells was successful. (helsinki.fi)
  • In 2013, the Nobel Prize in Physiology or Medicine was awarded to Randy W. Schekman, James E. Rothman, and Thomas C. Südhof for their discovery of how cells deliver thousands of membrane proteins to the right place at the right time. (bioquicknews.com)
  • The renal Na(+)/P(i) cotransporter NPT2 is expressed in the brush border membrane (BBM) of proximal tubular cells. (thermofisher.com)
  • Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
  • Expression of AQP5 in mouse cornea and transfected Madin-Darby canine kidney (MDCK) cells was detected using immunofluorescence or EGFP tag. (bvsalud.org)
  • Tail-anchored membrane proteins: investigating the targeting and membrane insertion mechanisms, which is important due to the wide range of core cellular functions performed by these proteins. (shu.ac.uk)
  • CALIXAR provides high-quality membrane protein identification services for scientists and researchers who need to explain the mechanisms of specific ligands in native environments, or to identify new membrane protein markers of specific diseases or pathologies. (calixar.com)
  • NPT2 gene expression is crucial for PTH effects on renal P(i) handling. (thermofisher.com)
  • While many studies have been focused on the membrane structure, the dynamics of such systems are crucial for the function of the membrane including membrane bound proteins. (lu.se)
  • Recently, Dr. Satoh's group performed the genome-wide screening of Drosophila mutants, and identified 233 mutants that failed to synthesize and/or transport rhodopsin to the photosensitive membrane of the rhabdomeres. (bioquicknews.com)
  • TMEM88 inhibits extracellular matrix expression in keloid fibroblasts. (nih.gov)