VectorsAdeno-assocTransductionTiterShRNATransgene expressionCellsGenesOverexpressionProteinsKnockdownInhibitionApoptosisInjectionTissuesFluorescenceHumanPromoterEndogenousProliferationAstrocytesApoptoticAutophagyDownregulationRegulatesTransgenicMicrogliaProtein expressionCell lineProductsSystemDelivery and ExpressionTranscription factorsSignificantly increasedCustomLevels
Vectors7
- In the present study, we aim the generation of Tet-On all-in-one lentiviral vectors (LVs) that tightly regulate transgene expression in human stem cells using the original TetR repressor. (nih.gov)
- Trans IT™-Lenti Transfection Reagent is designed to enhance delivery of packaging and transfer vectors to adherent HEK 293T cell types and increase recombinant lentivirus production. (fishersci.com)
- Well-regarded in the industry for high, reliable gene expression, SBI's lentiviral vectors also efficiently deliver RNAi. (systembio.com)
- For further information on sourcing optimized lentiviral vectors for your research please visit https://www.amsbio.com/lentivirus-orf-expression/ or contact AMSBIO on +44-1235-828200 / +1-617-945-5033 / [email protected] . (onenucleus.com)
- There are a number of hypothesized that regulating the transgene with a GFAP promoter in a different vectors that transduce cells in the brain in a slightly different viral vector would give rise to a high transgenic expression in the manner (for a review see, e.g. (lu.se)
- These vectors have a number of appealing features including the expression by using the machinery of the host cell instead of depending abilities to ef®ciently transduce cells in the central nervous system, on recombinant regulatory proteins. (lu.se)
- However, it is difficult to capture the complexity of transcript isoform variance using these libraries, and large cDNA sequences are often difficult to clone into size-limited viral expression vectors. (cdc.gov)
Adeno-assoc2
- In this review article, we outline key characteristics of major gene therapy viruses-adenovirus, adeno-associated virus (AAV), and lentivirus-and summarize the mechanisms regulating important steps in the virus journey from binding at peripheral nerve terminals to nuclear delivery. (frontiersin.org)
- these include high quality solutions for viral delivery (lentivirus, adenovirus and adeno-associated virus) in addition to GMP cryopreservation technology. (onenucleus.com)
Transduction2
- After a lentivirus preparation is complete, the titer will need to be determined prior to transduction. (sigmaaldrich.com)
- It is important to note that measuring the functional titer of a recombinant lentivirus is not only dependent on how efficiently the transfer vector is packaged but also the transduction efficiency of the cell line used. (sigmaaldrich.com)
Titer1
- This is the concentrated lentivirus with higher titer. (gentarget.com)
ShRNA8
- The human pancreatic cancer Capan-1 cell line was first infected with lentivirus-mediated shRNA and then analyzed by real-time PCR, and the results were further confirmed by Western blotting. (academicjournals.org)
- DcR3 protein in our experimental results showed significant expression among the pancreatic cancer cell lines and their decreased expression by lentivirus-mediated shRNA resulted in the inhibition of cell proliferation and augmentation of apoptosis. (academicjournals.org)
- A ) PC3 cells were infected with lentivirus expressing scrambled shRNA (C shRNA) or Bad-specific shRNA (Bad shRNA) or mock infected (no shRNA) for 2 days. (jci.org)
- Vector control- (V) and mouse Bad-expressing (mBad) LNCaP cells were infected with shRNA lentivirus as described above. (jci.org)
- Our HIV-based pGreenPuro (EF1α) shRNA Cloning and Expression Lentivector shRNA Cloning and Expression Lentivector drives expression of your shRNA template from the H1 promoter, and after processing in the cell, your shRNA will be converted into siRNA. (systembio.com)
- To produce siRNAs for RNAi using the pSIF1-H1-H2Kk Cloning and Expression Lentivector, first clone your shRNA template into the unique BamHI or EcoRI sites in the vector. (systembio.com)
- Generating siRNA from the pGreenPuro (EF1α) shRNA Cloning and Expression Lentivector. (systembio.com)
- shRNA Cloning and Expression Lentivectors. (systembio.com)
Transgene expression9
- Conditional transgene expression in human stem cells has been difficult to achieve due to the low efficiency of existing delivery methods, the strong silencing of the transgenes and the toxicity of the regulators. (nih.gov)
- To our knowledge, Lent-On-Plus is the first all-in-one vector system that tightly regulates transgene expression in bulk populations of human pluripotent stem cells and its progeny. (nih.gov)
- These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. (cshlpress.com)
- For successful transgene expression, viruses administered into muscle must undergo a series of processes, including host cell interaction and internalization, intracellular sorting, long-range retrograde axonal transport, endosomal liberation, and nuclear import. (frontiersin.org)
- The ability to regulate transgene expression will be crucial for development of gene therapy to the brain. (lu.se)
- We constructed a lentiviral vector that directs transgene expression to astrocytes. (lu.se)
- Using toxin-induced lesions we investigated to what extent transgene expression could be regulated in accordance with the activation of the endogenous GFAP gene. (lu.se)
- 2000). We of glial cell line-derived neurotrophic factor (GDNF) in models of have recently reported that this vector directs transgene expression to Parkinson's disease (Georgievska et al. (lu.se)
- We, developed is the ability to regulate transgene expression. (lu.se)
Cells16
- However, how DcR3 expression is modulated in pancreatic cancer cells is enigmatic. (academicjournals.org)
- C ) LNCaP cells were transfected with control vector or mouse Bad expression vector. (jci.org)
- Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. (cshlpress.com)
- Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. (cshlpress.com)
- Here we show that these mechanisms specifically require CC-chemokine receptor 2 (CCR2) expression in bone marrow cells (BMCs). (nih.gov)
- Moreover, cGAS is an important molecule for regulating basal expression levels of cell-intrinsic immune genes in cells and is, therefore, a central protein in immune responses to virus infection 10 . (nature.com)
- Pterygium epithelial cells (PECs) were cultured in keratinocyte serum-free defined medium to examine the expression of IPO13 and K17. (molvis.org)
- In this study, we observed that the expression of pseudouridine (Ψ) synthase 10 is increased in aged hematopoietic stem and progenitor cells (HSPC) and enforced protein of Ψ synthase 10 (PUS10) recapitulates the phenotype of aged HSC, which is not achieved by its Ψ synthase activity. (haematologica.org)
- Expression of human SSTR2 by lentivirus vector in T cells. (jci.org)
- the expression levels of protein associated with apoptosis and endoplasmic reticulum stress (ERS) of SKO-007 cells in various groups were detected by Western blotting method. (researchgate.net)
- Lentivirus can effectively transduce both dividing and non-dividing mammalian cells, and integrate into the host genome, allowing stable long-term, high-level gene expression both in vivo and in vitro . (technologynetworks.com)
- Unlike traditional retroviral system, the AMSBIO lentivirus is much more actively imported into the nuclei of non-dividing cells and stably integrated into the host cell's genome independent of cell cycle. (onenucleus.com)
- Dormant breast cancer cells exhibit a distinct gene expression signature regardless of metastatic site. (biomedcentral.com)
- In this study, we developed an efficient system for isolating rare dormant breast cancer cells in experimental metastases models and performed scRNA-seq comparing gene expression in dormant vs proliferative breast cancer cells. (biomedcentral.com)
- Our studies suggest that the gene expression signature we identified in disseminated dormant breast cancer cells exists in the primary site and can be utilized to predict which patients are more likely to experience a breast cancer recurrence. (biomedcentral.com)
- The stemness of the cells were characterized by the sphere-forming capacity and the expression levels of RSCs markers. (bvsalud.org)
Genes5
- We found a significantly lower expression of key autophagy- (ATG-) related genes in primary AML as compared to healthy granulocytes, an increased autophagic activity during all- trans retinoic acid- (ATRA-) induced neutrophil differentiation, and an impaired AML differentiation upon inhibition of ATG3, ATG4D, and ATG5. (hindawi.com)
- Results from RNA sequencing revealed that AEG‑1 modulated neuronal morphology and development by regulating the expression of numerous genes, such as ubiquitin C, C‑X‑C motif chemokine ligand 1, MMP9, Notch1, neuropilin 1 and ephrin type‑A receptor 4. (spandidos-publications.com)
- Expression of these genes had no impact on dormancy in murine models, but their expression correlated with disease-free survival in primary human breast cancer tumors, suggesting that these genes have predictive value in determining which patients are likely to recur. (biomedcentral.com)
- The expression of dormancy-related genes identified in our analyses was consistently observed among different breast cancer dormancy models and correlated with breast cancer progression in patients. (biomedcentral.com)
- These dCas9-activator fusions targeted to the promoter region of endogenous genes can then modulate gene expression 7 - 11 . (cdc.gov)
Overexpression3
- Lentivirus-mediated silencing and overexpression IPO13 testing was conducted, and K17 alternation was evaluated with western blot and immunostaining. (molvis.org)
- IPO13 was colocalized with K17 in the epithelium of the pterygium, and overexpression or knockdown of the IPO13 gene induced upregulation or downregulation of K17 expression in PECs, respectively. (molvis.org)
- Lentivirus-mediated MafF overexpression inhibited HCC cell proliferation and induced cell apoptosis. (techscience.com)
Proteins3
- Pseudotyping of lentivirus is available with wild type or mutant S proteins from a wide range of coronavirus species. (technologynetworks.com)
- C1QBP significantly increased ROS level, apoptosis, and the expression of apoptotic proteins such as cleaved caspase-3 and bax/bcl2 via regulating XDH. (medsci.org)
- Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
Knockdown1
- The AMSBIO lentiviral system is a novel gene delivery tool using lentivectors for gene expression or knockdown. (technologynetworks.com)
Inhibition2
- Inhibition of HSP90β expression improved neurological deficits and alleviated brain injury by increasing EAAT2 expression. (spandidos-publications.com)
- These results suggested that HSP90β is involved in the process of cerebral ischemia‑reperfusion injury in rats and that inhibition of HSP90β expression increases EAAT2 levels, conferring a neuroprotective effect in MCAO model rats. (spandidos-publications.com)
Apoptosis1
- The aim of this study was to characterize the expression of DcR3 in pancreatic carcinoma and to evaluate the role of DcR3 in cell proliferation and apoptosis, which may lead to the development of novel treatments for this disease. (academicjournals.org)
Injection2
- Lentivirus construction and injection were used to interfere with the expression of HSP90β. (spandidos-publications.com)
- Injection of CCR2 lentiviruses restored CCR2 expression and functions in monocytes. (nih.gov)
Tissues1
- Moreover, the expression of C1QBP and XDH was lower in RCC tumors compared with the tumor-associated normal tissues, and their down-regulation was associated with higher Fuhrman grade. (medsci.org)
Fluorescence1
- If using fluorescence: identify the well with desired quantifiable fluorophore expression at the lowest tested MOI value. (sigmaaldrich.com)
Human6
- Lentivirus express human codon-optimized Cas9 endonuclease ( Streptococcus pyogenes ) under the enhanced EF1a promoter , with a proprietary NLS (Nuclear Localization Signal) at its N-terminal, for the best nuclear penetration. (gentarget.com)
- GenTargetDescription:Premade lentivirus expressing human TERT gene under suCMV promoter, containing RFP-Puromycin marker.Storage conditions: (-80°C). (reportergene.com)
- GenTargetDescription:Concentrated lentivirus in PBS, expressing human TERT gene under suCMV promoter, containing RFP-Puromycin marker. (reportergene.com)
- A ) A schematic of the lentivirus vector encoding human SSTR2 is shown. (jci.org)
- found that with human sera known to contain antibodies to coron- the expression of M, E, or N, in the absence of S, did not avirus strains OC43 and 229E. (cdc.gov)
- Jakobsson expression under a human GFAP (hGFAP) promoter. (lu.se)
Promoter1
- The vector also co-expresses puromycin and copGFP from the moderate EF1α promoter, with co-expression mediated by a T2A element. (systembio.com)
Endogenous2
- In this study, we investigated the relationship between endogenous MafF expression and HCC progression and explored the regulatory mechanism of MafF expression in HCC. (techscience.com)
- An aspect of gene delivery to the brain that is currently being regulate the endogenous GFAP expression in a lesioned brain. (lu.se)
Proliferation1
- Silencing AEG-1 expression has also been shown to suppress AST migration and proliferation to wounded areas ( 11 ). (spandidos-publications.com)
Astrocytes1
- AEG‑1 is associated with glutamate excitotoxicity due to its reported function of repressing excitatory amino acid transporter 2 expression in astrocytes. (spandidos-publications.com)
Apoptotic1
- In addition, upregulation of AEG-1 expression can protect nigral dopaminergic neurons from injury caused by aberrant apoptotic signaling pathways ( 13 ). (spandidos-publications.com)
Autophagy2
- Low expression of the autophagy initiator ULK1 in AML can partially be attributed to high expression of the ULK1-targeting microRNA-106a. (hindawi.com)
- 2023) C/EBPβ Regulates TFAM Expression, Mitochondrial Function and Autophagy in Cellular Models of Parkinson's Disease. (systembio.com)
Downregulation3
- Downregulation in cellular ion homeostasis, including ion channel function and neurotransmitter release, were observed after knocking out AEG‑1 expression. (spandidos-publications.com)
- By contrast, in neurons, downregulation of AEG-1 expression has been shown to reduce the viability of motor neurons in models of amyotrophic lateral sclerosis (ALS), both in vivo and in vitro ( 12 ). (spandidos-publications.com)
- Ablation of Sox2 in the developing SCN leads to downregulation of circadian neuropeptides as early as embryonic day (E) 15.5, followed by a decrease in the expression of two transcription factors involved in SCN development, Lhx1 and Six6, in neonates. (bvsalud.org)
Regulates1
- The present study aimed to investigate the effects of changes in heat shock protein (HSP)90β expression and verify whether HSP90β regulates EAAT2 expression in a cerebral ischemia‑reperfusion injury model. (spandidos-publications.com)
Transgenic1
- Materials and methods approach to regulate transgenic expression. (lu.se)
Microglia3
- In addition, APPsα induced the recruitment of microglia with a ramified morphology into the vicinity of plaques and upregulated IDE and TREM2 expression suggesting enhanced plaque clearance. (springer.com)
- HSP90β expression coincided with astrocyte markers in the ischemic penumbra area, while no expression was observed in microglia. (spandidos-publications.com)
- Decreased CCR2 expression in bone marrow-derived microglia may therefore play a major role in the etiology of this neurodegenerative disease. (nih.gov)
Protein expression2
- Scope includes mutations and abnormal protein expression. (cancerindex.org)
- C1QBP promoted the mRNA and protein expression of hypoxanthine catabolic enzyme XDH. (medsci.org)
Cell line3
- Stable, long-term expression of your Custom Gene for your cell line of interest with custom selection marker (Puromycin, Blasticidin or Neomycin). (virostem.com)
- We designed a lentivirus for intracellular expression of the Kv1.3-selective peptide toxin agitoxin and created a Jurkat lymphocyte cell line that constitutively expressed intracellular agitoxin to selectively inhibit intracellular Kv1.3. (fortunepublish.com)
- We investigated the homing-related chemokine expression levels of MSCs treated with a prostate cancer cell line (PC-3) -conditioned medium. (cancerindex.org)
Products1
- Methods and tips for successful use of lentivirus products. (sigmaaldrich.com)
System1
- Gene manipulation was performed by lentivirus system. (bvsalud.org)
Delivery and Expression1
- Throughout our research - AMSBIO offered great support through discussing possible options in vector design for optimal delivery and expression our organoids. (onenucleus.com)
Transcription factors1
- Here, we report that the stem/progenitor cell transcription factor, Sex determining region Y-box 2 (Sox2), is required in the embryonic SCN to control the expression of SCN-enriched neuropeptides and transcription factors. (bvsalud.org)
Significantly increased1
- A gene expression array showed significantly increased expression of CCR3, a receptor of eotaxin-3, in PC-3. (cancerindex.org)
Custom2
- AMSBIO has extended its COVID-19 research portfolio with the introduction of a service to provide custom lentivirus pseudotyped with spike glycoprotein from SARS-CoV-2 to help researchers identify potential targets for development of therapeutic drugs and vaccines. (technologynetworks.com)
- We can offer a custom service to make ready-to-use constitutive or inducible over-expression lentivirus for any specific gene. (onenucleus.com)
Levels1
- Immunofluorescence was used to detect HSP90β expression localization and the expression levels of HSP90β and EAAT2 were determined using western blotting and reverse transcription‑quantitative PCR. (spandidos-publications.com)