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Saccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsFungal ProteinsGenes, FungalGene Expression Regulation, FungalSaccharomycesMolecular Sequence DataMutationRNA, FungalAmino Acid SequenceBase SequenceDNA, FungalChromosomes, FungalGenome, FungalCloning, MolecularPlasmidsGenetic Complementation TestSequence Homology, Amino AcidGene DeletionSuppression, GeneticTranscription, GeneticPhenotypeSpores, FungalDNA-Binding Proteinsbeta-FructofuranosidaseRestriction MappingTranscription FactorsGenes, SuppressorHaploidyMutagenesisVacuolesTransformation, GeneticFermentationRecombination, GeneticTemperatureDiploidyCell Cycle ProteinsKineticsMitochondriaSilent Information Regulator Proteins, Saccharomyces cerevisiaeRecombinant Fusion ProteinsSequence AlignmentSchizosaccharomycesProtein BindingGlucoseAllelesMembrane ProteinsMeiosisGenes, Mating Type, FungalDNA RepairSaccharomycetalesNuclear ProteinsKluyveromycesCDC28 Protein Kinase, S cerevisiaeSpheroplastsEscherichia coliAdenosine TriphosphatasesDNA PrimersRNA, MessengerGalactoseRecombinant ProteinsCell CycleRepressor ProteinsCell WallErgosterolCandida albicansKiller Factors, YeastBinding SitesPheromonesSubstrate SpecificityCarrier ProteinsOpen Reading FramesPromoter Regions, GeneticBiological TransportGenesSequence DeletionCulture MediaProtein Structure, TertiaryXyloseSirtuin 2Genes, LethalDNA ReplicationChitin SynthaseSchizosaccharomyces pombe ProteinsMembrane Transport ProteinsCathepsin AMitosisModels, BiologicalSequence Homology, Nucleic AcidChromosome MappingEthanolWineModels, GeneticCell NucleusConserved SequenceSpecies SpecificityTelomereMannosyltransferasesIndustrial MicrobiologyGenotype
GenesStrainsYeast SaccharomycesOrganismBaker'sProteinsS288cHeterologousEthanolFusion proteinRecombinantFunctionalTransferaseIncreased accumulationStressEndoplasmic reticulumGenome-wideCellulaseFattyQuantitativeYeastsSecretionWidelyProtein expressionMRNACelluloseStudyIncreasesHumanGalactoseBelongsEndoglucanaseFluorescencePathwayMembraneSystemCellPromoterEnvironmentsControl of geneHighLevelsSuc2Chromosomes
Genes14
  • constructed a co-expression vector containing two independent expression cassettes based on six restriction enzyme sites and achieved the co-expression of egv 3 (GenBank: HG313887.1) and cbh 2 (GenBank: HG313872.1) genes each controlled by a separate promoter in Trichoderma reesei [ 11 ]. (biomedcentral.com)
  • HE4 is one of more than 8900 different human genes represented on U95a GeneChips (Affymetrix, Santa Clara, CA, USA), which we used previously to develop a molecular classification of 175 human carcinomas based on patterns of gene expression. (nature.com)
  • We identified genes whose expression is correlated with the degree of thermotolerance in Saccharomyces cerevisiae by DNA microarray analysis. (springeropen.com)
  • Gene expression profiles of three S. cerevisiae strains showing different levels of thermotolerance were compared, and we chose three of them as candidate genes. (springeropen.com)
  • Among these genes, FMP21 was investigated as a thermotolerance-related gene in S. cerevisiae by comparing the growth at high temperature with the gene expression in eight strains. (springeropen.com)
  • Analyses of genes whose expression is induced by high temperature are important for understanding the mechanisms of thermotolerance, and for the use of the genes in breeding thermotolerant yeasts. (springeropen.com)
  • Genes related to the thermotolerance of S. cerevisiae have been identified by DNA microarray analyses (Eisen et al. (springeropen.com)
  • In the present study, we attempted to identify genes whose expression is correlated with the degree of thermotolerance in S. cerevisiae . (springeropen.com)
  • One of the most promising techniques to optimise cell factory performance is to control the expression/repression of relevant genes using dynamic regulation. (biomedcentral.com)
  • Previous efforts to implement dynamic regulation under industrially relevant conditions required the knockout of galactose utilization genes so that a small amount of galactose can be added to fermentations as a gratuitous inducer for gene expression [ 6 ]. (biomedcentral.com)
  • High concentrations of fermentable carbon sources repress gene expression from galactose promoters (GAL) via a carbon catabolite repression mechanism [ 7 ], and galactose utilisation genes are not activated during growth on sucrose [ 8 ]. (biomedcentral.com)
  • One basic problem is how a decrease in tRNA levels, by downregulating Pol III efficiency, influences the expression pattern of protein-coding genes. (mdpi.com)
  • Here we show that in Saccharomyces cerevisiae a single DSB causes transcriptional inhibition of proximal genes independently of Tel1/ATM and Mec1/ATR. (elifesciences.org)
  • A tetO Toolkit To Alter Expression of Genes in Saccharomyces cerevisiae. (bvsalud.org)
Strains3
  • The growth properties of S. cerevisiae at high temperatures differ according to the strain used, but the optimum temperature of proliferation is around 30°C. Some high-temperature-resistant strains have been isolated from tropical areas and obtained by mutational improvement, breeding, or the cell fusion of existing strains (Pasha et al. (springeropen.com)
  • We compared the gene expression profiles of three S. cerevisiae strains that showed different levels of thermotolerance. (springeropen.com)
  • S. boulardii is different from other strains of S. cerevisiae, commonly known as brewer's yeast and baker's yeast. (medlineplus.gov)
Yeast Saccharomyces7
Organism4
  • The eukaryotic model organism Saccharomyces cerevisiae is potentially a good expression host as it tolerates several stress conditions and is known to better express insoluble proteins compared to bacterial systems. (lu.se)
  • S. cerevisiae is an attractive model organism due to the fact that its genome has been sequenced, its genetics are easily manipulated, and it is very easy to maintain in the lab. (jove.com)
  • Now that we've learned a bit about S. cerevisiae as an organism, let's discuss what makes it a great model system for research. (jove.com)
  • Saccharomyces boulardii (S. boulardii) is a type of probiotic ("friendly" organism). (medlineplus.gov)
Baker's2
  • Saccharomyces cerevisiae (commonly known as baker's yeast) is a single-celled eukaryote that is frequently used in scientific research. (jove.com)
  • Saccharomyces cerevisiae , otherwise known as baker's yeast, is one of the many model organisms studied in laboratories all over the world. (jove.com)
Proteins5
  • Expression of αSyn in yeast results in growth impairment and cytoplasmic protein inclusions resembling the aggregates observed within LBs. A tandem fluorescent protein timer (tFT) was exploited to perform a high-throughput screen for proteins with altered turnover upon expression of αSyn or a phosphorylation-deficient S129A mutant. (uni-goettingen.de)
  • To characterize the change(s) in mRNA and protein expression of SIRT1-7 and their associated proteins in the brain of "physiologically" aged Wistar rats. (frontiersin.org)
  • We tested mRNA and protein expression levels of rat SIRT1-7, and the levels of associated proteins in the brain using RT-PCR and western blotting. (frontiersin.org)
  • Purifying the SNB-BAR proteins from yeast cells allows the acquisition of native homo and hetero SNX-BAR dimers while minimizing the toxicity and insolubility frequently encountered with non-native expression systems. (jove.com)
  • The replicase proteins p33 and p92 of Cymbidium ringspot virus (CymRSV) were found to support the replication of defective interfering (DI) RNA in Saccharomyces cerevisiae cells. (microbiologyresearch.org)
S288c1
Heterologous3
  • To solve this problem, significant expression and secretion levels of heterologous cellulases in yeasts must be achieved. (biomedcentral.com)
  • We used the glucose/sucrose system to control a heterologous RNA interference module and dynamically repress the expression of a constitutively regulated GFP gene. (biomedcentral.com)
  • Candida maltosa NADPH-cytochrome P450 reductase: cloning of a full-length cDNA, heterologous expression in Saccharomyces cerevisiae and function of the N-terminal region for membrane anchoring and proliferation of the endoplasmic reticulum. (mdc-berlin.de)
Ethanol1
Fusion protein1
  • Our initial assays with the L. starkeyi secretome expressing differing Tr EGII domains fused to a chimeric Talaromyces emersonii - T. reesei CBHI ( TeTr CBHI) showed that the complete Tr EGII enzyme, including the glycoside hydrolase (GH) 5 domain is required for increased expression level of the fusion protein when linked to CBHI. (biomedcentral.com)
Recombinant3
  • To reduce potentially toxic effect on the host cell, the growth and production phases were separated by using the inducible promoter GAL1p to control recombinant gene expression. (lu.se)
  • The recombinant vector was transformed into S. cerevisiae and was then induced by galactose. (ac.ir)
  • Expression of the L1 protein using recombinant DNA technology produces noninfectious virus-like particles (VLPs). (cdc.gov)
Functional3
  • Functional expression of CXCR4 in Saccharomyces cerevisiae in the development of powerful tools for the pharmacological characterization of CXCR4. (psu.edu)
  • Dive into the research topics of 'Functional expression of CXCR4 in Saccharomyces cerevisiae in the development of powerful tools for the pharmacological characterization of CXCR4. (psu.edu)
  • A wild-type strain, S. cerevisiae BY4743 and the deletion strain fmp21 Δ derived from BY4743 were obtained from EUROSCARF (EUROpean Saccharomyces Cerevisiae ARchive for Functional analysis). (springeropen.com)
Transferase1
  • Eucaryotic elongation factor 2 (EF-2) contains a post-translationally modified histidine residue termed diphthamide that is specifically ADP-ribosylated by diphtheria toxin (DT) or Pseudomonas exotoxin A. To analyze the potential physiological role of ADP-ribosylation of EF-2 by cellular ADP-ribosyl transferase, we constructed DT-resistant, non-ADP-ribosylatable Saccharomyces cerevisiae EF-2 by site-directed mutagenesis and expressed the mutant EF-2 in yeast. (nih.gov)
Increased accumulation1
  • Expression of αSyn upon deletion of SEM1 resulted in increased accumulation of ubiquitinated conjugates. (uni-goettingen.de)
Stress3
  • Using a randomized crossover study design, 16 adult pointer dogs were used to compare changes in fecal characteristics, oxidative stress marker concentrations, and gene expression when fed a SCFP-supplemented diet or control diet. (asas.org)
  • Blood cyclooxygenase-2 and myeloperoxidase mRNA gene expression was affected by diet during transport stress, being increased in control dogs, but stable or decreased in dogs fed SCFP. (asas.org)
  • A genome-wide expression analysis indicated decreased endoplasmic reticulum stress in the strain that moderately overexpressed SEC16, which was consistent with a decreased volume of the endoplasmic reticulum. (dtu.dk)
Endoplasmic reticulum1
Genome-wide1
  • 2003 ]) and a genome-wide screening of an S. cerevisiae deletion mutant collection (Auesukaree et al. (springeropen.com)
Cellulase1
  • The trifunctional cellulase gene ( BCE ) containing three types of cellulase functions was constructed and expressed in S. cerevisiae successfully. (biomedcentral.com)
Fatty1
  • SIRT3-5 expression declined significantly in the hippocampus and frontal lobe, associated with increases in superoxide and fatty acid oxidation levels, and acetylated CPS-1 protein expression, and a reduction in MnSOD level. (frontiersin.org)
Quantitative1
  • Genomic regions related to the high-temperature growth of S. cerevisiae were identified by quantitative trait locus (QTL) mapping (Steinmetz et al. (springeropen.com)
Yeasts1
Secretion1
  • We found that this new construct ( Tr EGII- TeTr CBHI, Fusion 3) had an increased secretion level of at least threefold in L. starkeyi compared to the expression level of the chimeric TeTr CBHI. (biomedcentral.com)
Widely1
  • Although a small number of 3' regulatory regions have been identified and validated so far, many studies have shown that plant 3' regulatory regions have a higher potential to regulate gene expression in plants compared to widely used 3' regulatory regions, such as NOS and OCS from Agrobacterium tumefaciens and 35S from cauliflower mosaic virus. (frontiersin.org)
Protein expression3
  • Here, we used oligonucleotide and tissue microarrays to survey HE4 gene and protein expression in a large group of malignant and normal tissues from adults. (nature.com)
  • While SIRT6 expression declines significantly with age acetylated H3K9 protein expression is increased throughout the brain. (frontiersin.org)
  • SIRT7 and Pol I protein expression increased in the frontal lobe. (frontiersin.org)
MRNA1
  • The 3' regulatory regions have a great diversity of cis -regulatory elements directly involved in polyadenylation, stability, transport and mRNA translation, essential to achieve the desired levels of gene expression. (frontiersin.org)
Cellulose1
  • Considering the demand for a synergistic action of multiple enzymes, the expression of a variety of enzymes in a single cell is likely to improve the efficiency of cellulose degradation [ 10 ]. (biomedcentral.com)
Study2
Increases1
  • Our data shows that SIRT1 expression increases with age, concurrently with increased acetylated p53 levels in all brain regions investigated. (frontiersin.org)
Human1
Galactose1
  • For example, the commonly used galactose induction system [ 4 ] has a noise level (expression in the absence of inducer) which is unacceptable for some applications such as controlling RNA interference [ 5 ]. (biomedcentral.com)
Belongs1
  • Along with mushrooms and molds, S. cerevisiae belongs to the Kingdom Fungi due to the presence of a cell wall made out of chitin, a polysaccharide polymer that's found not only in Fungi, but also in the exoskeletons of insects and crustaceans. (jove.com)
Endoglucanase2
  • "Comparison of the expression in Saccharomyces cerevisiae of endoglucanase II from Trichoderma reesei and endoglucanase I from Aspergillus aculeatus ," BioRes . (ncsu.edu)
  • Two distinct expression cassettes were synthesized by overlapping PCR for expressing the endoglucanase I gene ( egl 1) from Aspergillus aculeatus and the endoglucanase II gene ( egl 2) from Trichoderma reesei in a Saccharomyces cerevisiae host. (ncsu.edu)
Fluorescence1
  • The expression of IdeS was followed by constructing a fused protein with GFP and measuring the fluorescence with flow cytometry. (lu.se)
Pathway1
Membrane1
System2
Cell6
  • Saccharomyces cerevisiae fermentation product (SCFP) is a yeast product containing bioactive fermentation metabolites, residual yeast cells, and yeast cell wall fragments. (asas.org)
  • However, adenocarcinomas of the lung, and occasional breast, transitional cell and pancreatic carcinomas had moderate or high levels of HE4 expression. (nature.com)
  • Previous studies performed in S. cerevisiae that have contributed to our understanding of important cellular processes such as the cell cycle, aging, and cell death are also discussed. (jove.com)
  • On the other hand, S. cerevisiae undergoes cell division through a process called budding. (jove.com)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
  • In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
Promoter2
  • The ability to fine-tune the timing and population density at which gene expression is activated from the SUC2 promoter was demonstrated by varying the ratio of glucose to sucrose in the growth medium. (biomedcentral.com)
  • The expression plasmid pYtHSA-L7/L12 was constrcutred by inserting the L7/L12 gene into the pYHSA5 shuttle vector (Containing inulinase signal sequence, HSA gene and Gal10 promoter). (ac.ir)
Environments1
  • In nature, S. cerevisiae is found in warm, moist environments, with a source of sugar close at hand. (jove.com)
Control of gene1
  • Engineering dynamic, environmentally- and temporally-responsive control of gene expression is one of the principle objectives in the field of synthetic biology. (biomedcentral.com)
High1
Levels2
  • While expression levels of eL40A-HA from ubi1∆ub-HA are low, eL40A is produced practically at normal levels from the Smt3-S-eL40A-HA precursor. (123dok.net)
  • Microorganisms possess a variety of mechanisms for modulating gene expression levels according to changes in their environment. (biomedcentral.com)
Suc22
Chromosomes1