Expression in mammalian cells proteins clone cells. Medical search. Frequent questions

Novagen's pTriEx-1 vector contains bacterial, insect, and mammalian promoters. (the-scientist.com)
High-yield requirements might call for cloning of the target gene into an Escherichia coli expression vector, whereas subsequent activity studies might benefit from insect or mammalian cell expression. (the-scientist.com)
Until recently, testing of multiple systems was time consuming, involving cloning and verification of recombinants in each system-specific vector. (the-scientist.com)
The pTriEx-1 vector allows high-level gene expression in mammalian, E. coli , and insect cells. (the-scientist.com)
The HPNV major capsid protein (VP1) sequence was cloned into the mammalian expression vector pCAGGS by using EcoRI and XhoI sites. (cdc.gov)
Transfection of mammalian cells with an expression vector containing either TIF3 or TEF-1d cDNA resulted in overexpression of the encoded protein. (cdc.gov)
ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal DDK-tag. (origene.com)
The Gateway recombination cloning system is designed for highly efficient transfer of your DNA into any Gateway expression vector from a single entry clone. (thermofisher.com)
The Center for Cancer Systems Biology of the Dana-Farber Institute created a collection of human open reading frames (ORFs) cloned into a Gateway-entry vector, known as the CCSB Human ORFeome Collection. (horizondiscovery.com)
These clones are derived from fully-sequenced Mammalian Gene Collection (MGC) full-length cDNAs and subsequently cloned into a Gateway recombinational entry vector. (horizondiscovery.com)
PCR products were subsequently cloned into nmt promoter-driven expression vector pREP3x kindly provided by Dr. Susan Forsburg (Department of Biological Sciences at the University or college of Southern California Los Angeles CA) to obtain pREP3x-GIIα and pREP3x-GIIαVDEL. (exposed-skin-care.net)
The modified S-trimer was produced by synthesizing and cloning the codon-optimized gene into a mammalian expression vector. (news-medical.net)
The pQE TriSystem Vector allows for high-level expression of His-tagged proteins from a single vector containing three different expression systems. (qiagen.com)
The pQE-TriSystem vector contains CAG, T5, and p10 promoters that enable 6xHis-tagged protein expression in mammalian, E. coli , and baculovirus-infected insect cells, respectively (see figure pQE TriSystem. pQE TriSystem. "> pQE TriSystem ). (qiagen.com)
Vector pQE-TriSystem constructs can be transformed into E. coli , used as a shuttle vector for recombinant protein expression in insect cells, or transfected into mammalian cells. (qiagen.com)
The chimeric class I glycoprotein was expressed in target cells using an ALV derived retrovirus vector (RCASBP). (usda.gov)
The new pFastBac™ HBM TOPO™ vector enables secreted protein expression because it has the honeybee melittin (HBM) secretion signal. (fishersci.com)
The new vector should be strongly considered for the expression of glycoproteins. (fishersci.com)
The pFastBac™ HBM TOPO™ vector has also a C-terminal His-Tag with a TEV cleavage signal to enable easy purification of Histidine fusion proteins on nickel-chelating resins (ProBond™ Purification System) and native proteins with the aid of AcTev™ protease. (fishersci.com)
The vector uses the strong polyhedrin promoter to generate high levels of expression in a variety of insect cell lines such as Sf9, Mimic™ Sf9, Sf21, and High Five™ cells in Sf-900 II & Sf-900™ III media from Gibco. (fishersci.com)
Vector for expressing an N-terminally FLAG-tagged protein in mammalian cells. (addgene.org)
After cloning the antibody genes into an expression vector, this is then transfected into an appropriate host cell line for antibody expression. (cellsignal.com)
For expression in the Sf9 cells, the aim of the study was to clone the SLCO1B1-gene into the pFastBac vector. (helsinki.fi)
The synthesized gene was subsequently cloned into an expression vector. (cusabio.com)
After cloning, the expression vector was introduced into the Mammalian cell for expression. (cusabio.com)
METHODS: The null plasmid pcDNA-Vector and the overexpression plasmid pcDNA-ABHD11-AS1 were transfected into human gastric cancer cell lines MKN45 and MGC803 with low ABHD11-AS1 expression, and the changes in cell proliferation, colony formation, migration and invasion were examined using CCK-8 assay, colony formation assay and Transwell assay. (bvsalud.org)
With roughly 30,000 genes in mammalian genomes, fection with a vector encoding MyoD (Tapscott et al. (lu.se)

We offer protein expression and production services in baculovirus and mammalian host systems, as well as protein purification and analysis services. (thermofisher.com)
Vectors containing the preprotrypsin leader (PPT) sequence direct secretion of FLAG fusion proteins into the culture medium for purification using ANTI-FLAG antibodies, resins, and plates. (sigmaaldrich.com)
The HitS production process comprises steps for construct design, cloning and expression studies, purification evaluation, and quality control (QC) assessment. (bioprocessintl.com)
PEPCF expresses proteins in bacteria, insect and mammalian cells and uses a variety of chromatographic and biophysical techniques for protein purification and characterization. (embl.org)
We kindly request that you contact us first by phone, e-mail or video conference to discuss new expression and purification projects. (embl.org)
In case expression constructs and/or protocols are already present, you can immediately fill out our protein expression and purification request form and send the completed form back to us. (embl.org)
After we receive the sequence-verified expression constructs, we will first perform small scale expression and purification tests in which we assess the expression level, the solubility and the protein enrichment after a small scale affinity purification. (embl.org)
Once suitable conditions have been identified, we proceed to large scale expression (1-6 liter per construct) and protein purification using a variety of chromatographic techniques. (embl.org)
After harvesting the V1 virus, we perform small scale expression and purification tests, in which we assess the expression level, the solubility and the protein enrichment after a small scale affinity purification. (embl.org)
a)Essential Criteria 2A good knowledge of molecular (micro)biology, protein purification, and/or cell/tissue culture techniques (a), (c)Essential Criteria 3The ability to accurately and meticulously record procedures and results (both in notebooks and electronically). (ukvisago.com)
Expression and purification of proteins in yeast, E. coli, insect and mammalian cells. (lifeome.com)
ProteoGenix is a leading life sciences organization which provides services in molecular biology and immunology and in particular polyclonal and monoclonal antibody development, recombinant protein expression and purification, peptide synthesis as well as gene synthesis. (trialtop.com)
Using affinity purification-mass spectrometry and global phosphoproteomic and protein abundance analyses using three IAV strains (pH1N1, H3N2, H5N1) in three human cell types (A549, NHBE, THP-1), we map 332 IAV-human protein-protein interactions and identify 13 IAV-modulated kinases. (cdc.gov)

Target genes of interest cloned in the appropriate expression vectors are introduced into host cells. (weizmann.ac.il)
Known as the hORFeome v5.1, it contains over 15,000 individual clones representing over 12,000 unique genes. (horizondiscovery.com)
Expression of cloned envelope protein genes from the flavivirus tick-borne encephalitis virus in mammalian cells and random mutagenesis by PCR. (microbiologyresearch.org)
These studies aid in understanding how genes determining cell surface proteins can affect interactions between cells and confer viral disease resistance. (usda.gov)
Three introns were identified in the rainbow trout, Atlantic salmon and zebrafish genomic sequences, and the intron locations correspond closely to those in the mammalian TNFSF 13b genes. (usda.gov)
Whole exome sequencing of patients who experienced severe influenza reveals several genes, including scaffold protein AHNAK, with predicted loss-of-function variants that are also identified in our proteomic analyses. (cdc.gov)
explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)

Her expertise lies in protein biochemistry (including cloning, expression and characterization of proteins) and enzymology with a particular focus on structure, function and reactivity of mammalian peroxidases and their reaction products. (otago.ac.nz)
Bacterial artificial chromosome clones have been isolated from a trout library to further facilitate genomic characterization. (usda.gov)

For T7-regulated E. coli expression systems, MagicMedia Expression Medium can increase protein yields up to 10-fold. (thermofisher.com)
There is the T5 promoter/lac operator transcription-translation system for expression in E. coli, the p10 promoter for baculovirus-based expression in insect cells, and the CAG (CMV/actin/globin) promoter for expression in mammalian cells. (qiagen.com)
Preliminary studies can be carried out in bacterial expression systems, using the strong T5 promoter, which is recognized by E. coli polymerase, and allows efficient expression of proteins in any E. coli strain. (qiagen.com)
QIA express pQE vectors combine a powerful phage T5 promoter (recognized by E. coli RNA polymerase) with a double lac operator repression module to provide tightly regulated, high-level expression of recombinant proteins in E. coli . (qiagen.com)
Inserts encoding proteins of interest are cloned into appropriate constructs and transformed into a suitable E. coli strain for expression. (qiagen.com)
It relies on generation of recombinant virus by site-specific transposition in E. coli (DH10Bac™) rather than homologous recombination in insect cells. (fishersci.com)
Bac-to-Bac™'s pFastBac™ vectors, however, recombines with the parent bacmid in DH10Bac™ E. coli competent cells to form an expression bacmid. (fishersci.com)
For expression in E. coli , we accept all commonly used expression vectors. (embl.org)
For transposition of the gene of interest into the baculoviral DNA, we use E. coli DH10EMBacY cells. (embl.org)

Forward primer to sequence targets cloned in expression vectors. (origene.com)
Have us utilize our extensive suite of expression vectors and host systems to deliver the necessary protein yield for your application. (thermofisher.com)
Gateway technology allows highly efficient transfer of the gene of interest into any of a number of expression vectors from a single entry clone. (thermofisher.com)
The extremely wide variety of Gateway cloning-compatible expression vectors available makes the Gateway system of recombination cloning ideal for protein expression studies. (thermofisher.com)
Our selection of CMV vectors provides options for transient or stable expression of 3xFLAG ® fusion proteins. (sigmaaldrich.com)
Our mammalian expression vectors contain the strong CMV promoter for high-level constitutive expression in mammalian cells. (sigmaaldrich.com)
The pQE vectors (see table and figure pQE Vectors. Numbered elements listed in table. "> pQE Vectors ) enable placement of the 6xHis tag at either the N- or C-terminus of the recombinant protein. (qiagen.com)
It includes high-performing CHO cell lines, paired media and feeds, optimised expression vectors and robust protocols. (manufacturingchemist.com)
We do not offer any cloning services, but we do maintain a large collection of expression vectors. (embl.org)
All non-commercial expression vectors generated at EMBL are also available to external academic users via a Material Transfer Agreement . (embl.org)
For baculovirus-mediated expression in insect cells, we require expression vectors compatible with the Bac-to-Bac system (for example pFastBac, MultiBac, biGBac or MacroBac vectors). (embl.org)
For mammalian cells, we can work with most standard mammalian expression vectors (e.g. based on the pcDNA backbone) or BacMam vectors. (embl.org)

These include bacterial system and three eukaryotic systems, such as yeast, insect and mammalian cells. (weizmann.ac.il)
These include cell-free extracts ( in vitro expression systems), and bacterial, yeast, insect, and mammalian cell systems, each with its own advantages and drawbacks. (thermofisher.com)
Here we statement that in the absence of GIIβ the catalytic subunit GIIα of the fission yeast WYE-687 (an organism displaying a glycoprotein folding quality control mechanism similar to that occurring in mammalian cells) folds to an active conformation able to hydrolyze does not display a consensus ER retention/retrieval sequence. (exposed-skin-care.net)
cells were produced at 28°C in rich YES medium (0.5% yeast extract 3 glucose and 75 mg/l adenine) or Edinburgh minimal medium (EMM) (Moreno strains were produced at 28°C in rich media (YPDA 1 yeast extract 2 bactopeptone 2 glucose and 20 mg/l adenine) or selective minimal media SD (0.67% yeast nitrogen base without amino acids and 2% glucose) plus appropriate supplements for selective growth. (exposed-skin-care.net)
Yeast Total Protein Extract and Microsomal Portion Preparations Yeast whole cell extracts were prepared from 20 ml of exponentially growing cultures (for 20 min. (exposed-skin-care.net)
Currently we do not offer protein expression in other host organisms, but we can purify proteins expressed in other host organisms (e.g. yeast, hybridoma cell lines, …) if you are able to provide us with the expression cell pellet (intracellular proteins) or with the cell culture supernatant (secreted proteins). (embl.org)
Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.com)

First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. (mdpi.com)

Nevertheless whereas constitutes an ideal organism to study the role of GIIβ because it has a glycoprotein folding quality control mechanism similar to that occurring in mammalian cells and it expresses an active GT (Fernández DH5α and JA226 were utilized for cloning purposes whereas recombinant protein expression was carried out using BL26 cells. (exposed-skin-care.net)

We use a combination of cell-based and in vitro assays to show that the interface of the FluPol A dimer is required for vRNA synthesis during replication of the viral genome. (nature.com)
Furthermore GIIα was also retained in the ER of GIIβ null mutants and G1M9 was the and that microsomes WYE-687 from ΔGIIα and ΔGIIβ mutant cells are devoid of GII activity when using G1M9 as substrate in the assays. (exposed-skin-care.net)
As of July 2022, the unit has produced assays for more than 1,500 recombinant proteins, including multisubunit complexes, DNA- and RNA-modifying proteins, and membrane proteins. (bioprocessintl.com)
Drug developers can leverage such assays for focused screening activities - e.g., to determine whether a chemical entity binds with a given protein. (bioprocessintl.com)
Specific experiments will deal with DNA cloning and sequencing, enzyme mutagenesis, protein expression and enzyme assays, the identification of proteins in mammalian sera and using fluorescent microscopy to determine the subcellular localisation of proteins in mammalian cells. (edu.au)
Equipped with high-content analysis technologies, the platform focuses on live phenotypic screening in co-cultures of immune and cancer cells, as well as molecular cell-based assays for the assessment of immune checkpoint inhibitors. (trialtop.com)

The molecular mechanisms potentially responsible for cadmium carcinogenesis were investigated by differential gene expression analysis of Balb/c-3T3 cells morphologically transformed with cadmium chloride. (cdc.gov)
Dharmacon is a distributor of multiple gene expression clone collections (cDNAs and ORFs). (horizondiscovery.com)
First, we will outline the steps for establishing a tightly regulated gene expression system in human HEK293S cells. (essex.ac.uk)
Qu W , Jin H , Chen B , Liu J , Li R , Guo W , Tian H , . CPEB3 regulates neuron-specific alternative splicing and involves neurogenesis gene expression. (aging-us.com)
CPEB3 overexpression had insignificant effects on gene expression in HT22 cells. (aging-us.com)
High-throughput RNA sequencing (RNA-seq) has revolutionized our understanding of gene expression. (cdc.gov)
states'' or gene expression patterns. (lu.se)
In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)

If expression in mammalian or insect cells is required - to obtain post-translational modifications, for example - the same construct can be used without the need for time-consuming subcloning procedures. (qiagen.com)
Bac-to-Bac™ Baculovirus Expression is an efficient method for producing baculovirus for high-level protein expression in insect cells. (fishersci.com)
Transfect the bacmid into insect cells for fast production of a high titer of pure recombinant baculovirus particles in the very first transfection. (fishersci.com)

In contrast to glycoproteins secreted from mammalian cells, glycoproteins secreted from baculovirus can be easily de-glycosylated in vitro. (fishersci.com)
OATP2B1 variants were expressed in human derived HEK293 cell line using baculovirus expression system. (helsinki.fi)
The wild-type SLCO1B1-gene encoding the OATP1B1 was virulent with baculovirus into the cells by the Bac-to-Bac® Baculovirus Expression System. (helsinki.fi)

V2_F Primer can be used to sequence the target sequence after cloning. (origene.com)
We perform detailed comparative sequence-based-structure analysis of related proteins and correlate the protein structure to its function, biochemical findings and biological importance. (weizmann.ac.il)
Dharmacon has not sequence verified each individual clone from these collections. (horizondiscovery.com)
This composite promoter comprises the Elongation Factor-1α (EF-1α) core promoter [3] and the R segment and part of the U5 sequence (R-U5') of the Human T-Cell Leukemia Virus (HTLV) Type 1 Long Terminal Repeat [4]. (invivogen.com)
A chicken gene that produces a protein important for viral disease resistance was obtained and its DNA sequence was determined. (usda.gov)
We identified zebrafish and Fugu TNFSF 13b homologues by genomic DNA database searches and determined that the predicted proteins share 68% and 76% sequence identity with trout TNFSF 13b. (usda.gov)
After we receive the sequence-verified expression constructs, we will first generate the recombinant baculoviruses. (embl.org)
Sequence-specific RNA-Binding Protein CPEB3 has distinctive isoform-distinct biochemical interactions and neuronal development assembly roles. (aging-us.com)
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. (cusabio.com)

Our integrated offering accelerates therapeutic protein and antibody production and offers seamless tech transfer for optimal flexibility. (manufacturingchemist.com)
We sought to identify members of the tumor necrosis factor superfamily (TNFSF) because of their important roles in the regulation of mammalian B cell survival and antibody production. (usda.gov)

The constructs were electroporated into and expression levels were regulated with thiamine added to the media. (exposed-skin-care.net)
Protein synthesis is effectively blocked in the presence of high levels of lac repressor and the stability of cytotoxic constructs is enhanced. (qiagen.com)
mass spectrometry to identify proteins and the maintenance and manipulation of mammalian cell cultures, including the introduction of reporter constructs. (edu.au)
In case no pre-existing expression constructs and/or protocols are available, we can design a strategy together with you. (embl.org)

Cy3-labeled versions of both peptides were localized in the endothelial cells of arterial vessels in the rats. (go.jp)
The accumulation of both peptides in the endothelial cells suggested in vivo inhibitory activity of the angiotensin I-converting enzyme. (go.jp)
AoproS8 showed optimal activity at pH 9.0 and 40 °C. It was used for the production of xanthine oxidase (XOD)-inhibitory peptides from eight food processing protein by-products. (bvsalud.org)

One of the major aims of Dr. Ebihara's research project are to obtain a more detailed understanding of the biophysical properties of cloned lens gap junctional proteins and to localize the molecular determinants of permeability and gating of the gap junctional channels. (rosalindfranklin.edu)
Up-regulation of expression of translation factors - a novel molecular mechanism for cadmium carcinogenesis. (cdc.gov)
These findings demonstrate that the up-regulation of expression of TIF3 and TEF-1d is a novel molecular mechanism responsible, at least in part, for cadmium carcinogenesis. (cdc.gov)
This subject provides practical training in the technologies of molecular biology, protein expression and molecular cell biology. (edu.au)
Activated protein C resistance and low molecular weight lipoprotein (a): dual pathogens for atherothrombosis? (mcmaster.ca)
This CD38 protein migrated along the gel to a band of about 58-90 kDa molecular weight. (cusabio.com)
OBJECTIVE: To explore the role of long non-coding RNA ABHD11-AS1 in regulation of glycolysis in gastric cancer cells and its molecular mechanism. (bvsalud.org)

The full length cDNAs for TIF3 and TEF-1d were cloned and sequenced. (cdc.gov)

Monoclonal antibodies to the FLAG epitope served to monitor cell surface expression for functional analysis of the BFIV21 class I glycoprotein. (usda.gov)
Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.com)
In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. (cellsignal.com)

Today, the unit provides a rapid and cost-effective platform for protein generation, screening, biophysical and biochemical analysis, structural determination, and "hit" confirmation. (bioprocessintl.com)

1995) and subsequently in primary cells (Heyworth et al. (lu.se)

Furthermore, peripheral blood mononuclear cells (PBMCs) or splenocytes harvested from the vaccinated animals 14 days after the second vaccine dose and analyzed through flow cytometry were used for intracellular cytokine staining assay and enzyme-linked immunospot (ELISPOT) assay to examine the immune responses elicited by the vaccines. (news-medical.net)
The activated receptor catalyzes removal of GDP from the G protein alpha subunit (Gα i ), which allows intracellular GTP to bind. (elifesciences.org)

In this study the characteristics of the OATP1B1 transporter were reviewed and new in vitro research method to study protein functions was developed. (helsinki.fi)
The Sf9 cell line is isolated from Spodoptera frugiperda insect and is one of the standard in vitro tools in a genetic engineering study. (helsinki.fi)

mCFP is mainly intended for protein labeling in protein localization and interaction studies. (origene.com)
This fusion protein can be used to study the localization of TLR1. (invivogen.com)
What is the cellular localization of the protein? (embl.org)
However, further studies on the expression level and cellular localization of OATP2B1 variants are needed to fully characterize the impact of the variants studied. (helsinki.fi)

Curia has signed a licensing agreement with MilliporeSigma, the North American Life Sciences Business of Merck, Darmstadt, Germany, that enables Curia and its clients to use the CHOZN GS-/- cell line for production of therapeutic proteins and antibodies. (manufacturingchemist.com)
Transfected cells can be analyzed for GFP expression by flow cytometry and by Western-blotting using GFP antibodies. (invivogen.com)
While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.com)
Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
In contrast, antibodies to combinations of phospholipids and serum proteins (eg, β 2-glycoprotein I [β 2-GPI] or prothrombin) are more likely associated with the vasculopathic events of antiphospholipid antibody syndrome (APS). (medscape.com)

Mutagenesis of the NS2B-NS3-mediated cleavage site in the flavivirus capsid protein demonstrates a requirement for coordinated processing. (microbiologyresearch.org)

Stimulated muscarinic acetylcholine receptors (M2Rs) release Gβγ subunits, which slow heart rate by activating a G protein-gated K + channel (GIRK). (elifesciences.org)
These two branches control heart rate by stimulating different G protein-coupled receptors (GPCRs), which in turn activate ion channels that modify the electrical properties of cardiac pacemaker cells ( DiFrancesco, 1993 ). (elifesciences.org)

Expression is screened on a small scale in 4-10 ml cultures and soluble and insoluble cellular fractions are analyzed by SDS-PAGE for protein expression. (weizmann.ac.il)
Once optimal conditions have been determined, large-scale cultures (1-5L) are used to obtain larger amounts of protein. (weizmann.ac.il)

In this chapter, we focus on the construction of stable mammalian cell lines to overcome this hurdle. (essex.ac.uk)
The nature of the stem cell substates and their relationship to commitment to differ- entiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
Evidence the fate of stem cells has broad ramifications for biomedical suggests that during development or differentiation, cells make science from elucidating the causes of cancer to the use of very precise transitions between apparently stable ``network stem cells in regenerative medicine. (lu.se)

Does the native protein have any post-translational modifications? (embl.org)

It has been recently shown that mutations of lens gap junction proteins are associated with certain forms of inherited congenital cataract. (rosalindfranklin.edu)
In a recent study published in the journal npj Vaccines , researchers in China designed, produced, and evaluated the neutralization efficacy of a recombinant coronavirus disease 2019 (COVID-19) vaccine containing four hot-spot substitution mutations based on a prefusion-stabilized severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein (S) trimer. (news-medical.net)
The World Health Organization (WHO) has classified the SARS-CoV-2 Alpha, Beta, Gamma, Delta, and Omicron variants as variants of concern because they carry mutations in their spike protein that increase their transmissibility and ability to evade vaccine-induced immunity, as compared to the ancestral Wuhan strain. (news-medical.net)
The present study used the HexaPro (S-6P) S-trimer and an Alum/cytosine phosphoguanine (CpG) 7909 dual adjuvant system to create a modified spike trimer vaccine containing the four spike protein substitution mutations commonly found in the five variants of concern. (news-medical.net)
Mutations encoding basic (Arg), non-polar (Leu and Pro) and uncharged polar (Asn, Gln and Tyr) amino acids at M39 were introduced into a DENV-2 genomic-length cDNA clone and their effects on virus replication were examined. (microbiologyresearch.org)
Selected mutations were introduced into a prM-E gene cassette and expressed transiently in COS cells to investigate whether the mutations impaired prM/E association or secretion. (microbiologyresearch.org)
Accumulating evidence shows that tRNA expression and mutations are associated with various diseases such as neurological pathologies and cancer development3,4. (cdc.gov)

The trimeric conformation and purity of the modified spike protein were tested using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and size-exclusion high-performance liquid chromatography (HPLC). (news-medical.net)

Bioprocessing begins upstream, most often with culturing of animal or microbial cells in a range of vessel types (such as bags or stirred tanks) using different controlled feeding, aerating, and process strategies. (bioprocessintl.com)
Assay-grade proteins can be expressed using microbial, insect, or mammalian cell lines, and regardless of host, production activities typically can be performed in four to eight weeks. (bioprocessintl.com)
With its end-to-end capabilities, Stelis handles development stages ranging from pre-clinical to commercial for recombinant proteins expressed in both microbial and mammalian systems. (pharmaceutical-business-review.com)
The cell line and process development in microbial and mammalian systems is supported by extensive physicochemical and bio-analytical characterisation capabilities. (pharmaceutical-business-review.com)
Stelis BioSource has invested in high-performance technologies that support robust and efficient processes for both microbial and mammalian systems. (pharmaceutical-business-review.com)
The company's scale-up and tech-transfer services include optimising bioprocesses based on quality-by-design (QbD) principles, developing process analysis technology (PAT) for both mammalian and microbial processes, performing efficient tech-transfer to either customers' or Stelis BioSource's manufacturing facilities, and providing process characterisation studies using scale-down models. (pharmaceutical-business-review.com)
Stelis BioSource's state-of-art, 200,000 ft 2 , commercial-scale bio-manufacturing facility in Bangalore has the capability to produce simple and complex recombinant biotherapeutics from both microbial and mammalian cell culture systems. (pharmaceutical-business-review.com)

To determine the structural requirements for beta 4GalT7 activity, we have cloned and expressed the enzyme and designed a focused library of 2-naphthyl beta-D-xylopyranosides with modifications of the xylose moiety. (lu.se)
Author Manuscript and RNA repair protein AlkB-derived enzyme mixture was first used to remove methylations at the Watson-Crick face. (cdc.gov)

The spike (S) protein of SARS-CoV-2 mediates viral receptor binding and membrane fusion, both of which are essential for viral infection of host cells. (nature.com)
The mature flavivirus particle comprises a nucleocapsid core surrounded by a lipid bilayer containing the membrane (M) (derived from the precursor prM) and envelope (E) proteins. (microbiologyresearch.org)
The large-scale expression of many membrane proteins, including the members of the G protein-coupled receptor superfamily, in a correctly folded and fully functional form remains a formidable challenge. (essex.ac.uk)
Investigating the role of cell membrane proteins has increased over the last decade, as drugdrug interactions and genetic polymorphisms have been found to cause changes in drug pharmacokinetics and dynamics. (helsinki.fi)
Very lipophilic drugs enter the cell membrane through passive diffusion, but polar or ionized organic compounds can enter the cell membrane only by transporters. (helsinki.fi)
C ) A representative voltage-clamp recording from the same SAN cell in ( B ). The membrane potential was held at −80 mV, and 1 µM Iso or ACh was applied as indicated. (elifesciences.org)

Requirements for protein solubility, functionality, and yield are often the most important factors to consider when choosing an expression system, but the time and labor required for subcloning and optimizing expression can also have a significant impact. (thermofisher.com)
Therefore, the risk of altering protein function, blocking other epitopes, or decreasing solubility is minimized. (sigmaaldrich.com)

We also show that a nanobody (a single-domain antibody) that interferes with FluPol A dimerization inhibits the synthesis of vRNA and, consequently, inhibits virus replication in infected cells. (nature.com)
Synthesis and secretion of recombinant tick-borne encephalitis virus protein E in soluble and particulate form. (microbiologyresearch.org)
tRNAs are essential for cells and their synthesis is under stringent cellular control. (cdc.gov)

Using the best expression system for your protein and application is key to your success. (thermofisher.com)
The variety of expression choices available makes the Gateway system of recombination cloning ideal for protein expression studies. (thermofisher.com)
Detection of fusion proteins containing 3xFLAG ® is enhanced up to 200 times more than any other system. (sigmaaldrich.com)
CHOZN platform is a Chinese Hamster Ovary (CHO) mammalian cell expression system that allows for faster, simpler selection and scale-up of high-producing clones for production of biologics and therapeutic recombinant proteins. (manufacturingchemist.com)
Next, we describe the assembly of this expression system into HEK293S cells and a derivative cell line devoid of complex N-glycosylation. (essex.ac.uk)
1990). Use of the human elongation factor 1 alpha promoter as a versatile and efficient expression system. (invivogen.com)
1988). SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. (invivogen.com)
CRISPR/Cas9 system-mediated multi-copy expression of an alkaline serine protease (AoproS8) from Aspergillus oryzae was successfully built in Aspergillus niger. (bvsalud.org)
2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)
This requirement strongly limits the number of solutions or entiation and lineage-specification, programmed cell death, and ``states'' for the system. (lu.se)

Finally, we describe methods for the growth of these cells lines in scalable suspension culture for the preparation of milligram amounts of recombinant protein. (essex.ac.uk)

Then, a high protease activity of 11,023.2 U/mL with a protein concentration of 10.8 mg/mL was obtained through fed-batch fermentation in a 5 L fermenter. (bvsalud.org)
High temperature requirement factor A4 (HtrA4), a placental-specific protease, is responsible for protein quality control and placental syncytialization. (bvsalud.org)

The company's early stage development offerings include cell banking and characterisation. (pharmaceutical-business-review.com)

To increase the probability that a given protein will be expressed in soluble, active form, and in high yield several expression systems are used. (weizmann.ac.il)

The TLR1-GFP fusion gene is cloned in the pUNO plasmid under the control of the strong and ubiquitous mammalian promoter EF1α/HTLV. (invivogen.com)
GFP fusion gene has been fully sequenced, its fluorescence confirmed and its function tested in HEK293 cells coexpressing an NF-κB reporter plasmid ( pNiFty-SEAP ). (invivogen.com)

Immunoglobulins were detected by incubation with biotin-conjugated protein A (1 h at room temperature, Sigma-Aldrich, St. Louis, MO, USA), followed by an incubation step with streptavidine-horseradish peroxidase. (cdc.gov)

Dr Paumann-Page's main research interest is how the reactions of mammalian peroxidases are involved in health and disease. (otago.ac.nz)

GeneArt™ Cell Lines and Proteins are a suite of service offerings designed to help facilitate protein expression in mammalian cell lines. (thermofisher.com)
For less potent cell lines, protein levels are typically ~0.1 mG/L. The presence of the SV40 replication origin will result in high levels of DNA replication in SV40 replication permissive COS cells. (sigmaaldrich.com)
Dr Paumann-Page is investigating well-characterized cell lines from the New Zealand melanoma cell panel to characterize peroxidasin expression levels, activity, cellular location and the effect of modulation of peroxidasin protein levels and activity on cell invasiveness. (otago.ac.nz)
For protein expression we use either the Sf21 or the Hi5 insect cell lines. (embl.org)
In the experimental part of this thesis the goal was to express OATP1B1 transporter in Sf9 and HEK293 cell lines. (helsinki.fi)
Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)

The mechanism by which peroxidasin contributes to tumour cell invasion is unknown, but most likely connected to its enzymatic activity. (otago.ac.nz)
In the mammalian brain, alternative pre-mRNA splicing is a fundamental mechanism that modifies neuronal function dynamically where secretion of different splice variants regulates neurogenesis, development, pathfinding, maintenance, migration, and synaptogenesis. (aging-us.com)

The results of this study pinpoint a critical residue in the M protein that potentially plays a role in viral morphogenesis, secretion and entry. (microbiologyresearch.org)

Activation of endothelial cells via antibody-enhanced dengue virus infection of peripheral blood monocytes. (microbiologyresearch.org)

The strong human cytomegalovirus (CMV) promoter regulatory region drives constitutive protein expression levels as high as 1 mG/L in COS cells. (sigmaaldrich.com)

Confocal immunofluorescent analysis of transgenic A549 cells stably expressing SARS-CoV-2 nucleocapsid (N) protein (A549-N protein) (left, positive) or SARS-CoV-2 envelope (E) protein (A549-E protein) (right, negative), using SARS-CoV-1/2 Nucleocapsid Protein (1C7C7) Mouse mAb (green), DyLight ™ 554 Phalloidin #13054 (red), and DAPI #4083 (blue). (cellsignal.com)

A genome-scale library of Gateway-adapted human ORF clones, the CCSB Human ORFeome Library (aka hORFeome V5.1) is ideal for transferring thousands of open reading frames into a variety of protein expression systems. (horizondiscovery.com)
TLR1-GFP fusion gene was generated by fusing the green fluorescent protein (GFP) gene to the C terminus of human Toll-like receptor 1 (TLR1) gene. (invivogen.com)
The trout TNFSF 13b predicted protein shares 54% amino acid identity with human and chicken TNFSF 13b, and 51% identity with mouse TNFSF 13b. (usda.gov)
cDNA cloning and expression analysis of a novel human F-box domain containing gene. (nih.gov)
Human Embryonic Kidney cells (HEK) is a human derived mammalian cell-line that is widely used in the study of OATP1B1 transporter. (helsinki.fi)
The product was purified to obtain the recombinant human CD38 protein carrying C-terminal hFc tag. (cusabio.com)
CD38 is a gene providing instruction of making a protein named ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1 (also abbreviated as ADPRC1 or CD38) in human and belongs to ADP-ribosyl cyclase family. (cusabio.com)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
This study provides a comprehensive network model of IAV infection in human cells, identifying functional host targets for pan-viral HDT. (cdc.gov)

Use of recombinant proteins varies widely - from functional studies in vivo to large-scale protein production for structural studies and therapeutics. (thermofisher.com)

Meins reflected that WuXi AppTec established the HitS Unit 15 years ago to facilitate drug discovery for protein biologics. (bioprocessintl.com)

To varying degrees, these fates also extend to the Such state stability is required in stem and progenitor cells to immediate progeny of stem cells, known as progenitor or support self-renewal and maintenance of the uncommitted transit-amplifying cells. (lu.se)

Mammalian cells produce PGs, which are involved in biological processes such as cell growth and differentiation. (lu.se)
Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)

Obtaining the perfect balance between yield and activity may also inspire testing of several different expression systems. (the-scientist.com)

In contrast to recent data with chimeric, recombinant SARS-CoV-2 strains expressing the spike proteins of BA.1 and BA.2 on an ancestral WK-521 backbone 4 , we observed similar infectivity and pathogenicity in mice and hamsters for BA.2 and BA.1, and less pathogenicity compared with early SARS-CoV-2 strains. (nature.com)
Furthermore, AoproS8 was continuously knocked in the glaA, amyA, and aamy gene loci in A. niger to construct multi-copy expression strains. (bvsalud.org)

To investigate the physical structure of the chicken class I glycoprotein we have cloned the cDNA representing the BFIV locus of the B21 haplotype. (usda.gov)

Anatomy37

Organisms24

Diseases2

Chemicals and Drugs82

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes76

Disciplines and Occupations1

Information Science4

Health Care2

Vector27

Purification13

Genes7

Characterization2

Coli9

Vectors12

Yeast7

Bacterial1

Recombinant protein expression1

Assays6

Gene expression8

Insect cells3

Baculovirus3

Sequence9

Antibody production2

Constructs4

Peptides3

Molecular7

Full length cDNAs1

Monoclonal Antibodies3

Biophysical1

Subsequently1

Intracellular2

Vitro2

Localization4

Antibodies5

Mutagenesis1

Receptors2

Cultures2

Stable4

Post-translational1

Mutations7

Purity1

Microbial7

Enzyme2

Membrane6

Solubility2

Synthesis3

System10

Suspension1

Protease2

Characterisation1

Soluble1

Plasmid2

Sigma-Aldrich1

Reactions1

Lines6

Mechanism2

Secretion1

Endothelial cells1

Constitutive1

Nucleocapsid Protein1

Human10

Functional1

Biologics1

Stem and progenitor cells1

Differentiation3

Yield1

Strains2

Glycoprotein1