• Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is an alternative splicing factor that is mainly nuclear, although it shuttles rapidly between nuclear and cytoplasmic compartments. (pasteur.fr)
  • Our findings suggest that the phosphorylation of F-peptide by cell-signaling pathways regulates the rate of hnRNP A1 nuclear import. (pasteur.fr)
  • These motifs are specifically recognized by heterogeneous nuclear ribonucleoprotein A2 (hnRNP A2), a trans-acting factor required for dendritic delivery. (immuquest.com)
  • Of the 15 hnRNP proteins analyzed here, hnRNP A2, hnRNP F, and hnRNP H efficiently induced HPV16 late gene expression, and all of the hnRNPs affected HPV16 L1 mRNA levels or mRNA splicing. (lu.se)
  • La proteína hnRNP tiene una fuerte afinidad por el ARN rico en polipirimidina y por el ADN de hebra única rico en polipirimidina. (bvsalud.org)
  • Existen múltiples isoformas de la proteína K hnRNP debido a ayuste alternativo y muestran características diferentes en la unión al ácido nucleico. (bvsalud.org)
  • Moreover, individual fetal brains were analyzed by LC-MS/MS. Statistical differences in the amounts of proteins between the ALC and ALC/ADNF-9 groups resulted in a distinct data-clustering. (biomedcentral.com)
  • Significant upregulation of several important proteins involved in brain development were found in the ALC/ADNF-9 group as compared to the ALC group. (biomedcentral.com)
  • Label-free quantitative proteomic analyses using liquid chromatography in conjunction with a tandem mass spectrometry (LC-MS/MS) system showed significant alteration of mitochondrial, cytosolic, nuclear and cytoskeletal proteins in fetal brains exposed prenatally to alcohol [ 17 ]. (biomedcentral.com)
  • The team began by searching for essential factors to splice out human short introns from 154 human nuclear proteins. (innovitaresearch.com)
  • They downregulated these proteins' expression in a human cell line (HeLa cells) using small interfering RNAs (siRNA). (innovitaresearch.com)
  • We found that biallelic GEMIN5 variants cause structural abnormalities in the encoded protein and reduce expression of snRNP complex proteins in patient cells compared with unaffected controls. (ox.ac.uk)
  • Methods and results Using label-free LC-MS/MS, it was found that expressions of 1266 proteins were sig- nificantly changed between the experimental groups. (mdm2-receptor.com)
  • These characteristics correlate with a distinct nuclear architecture, epigenetic signatures enriched for active chromatin marks and hyperdynamic binding of structural chromatin proteins. (biomedcentral.com)
  • We have determined the effect of seven serine- and arginine-rich (SR) proteins and 15 heterogenous nuclear ribonucleoproteins (hnRNPs) on human papillomavirus type 16 (HPV16) late gene expression. (lu.se)
  • Of the seven SR proteins analyzed here, SRSF1, SRSF3, and SRSF9 induced HPV16 late gene expression, and five of the SR proteins affected HPV16 L1 mRNA splicing. (lu.se)
  • Strict control of the expression of the immunogenic L1 and L2 capsid proteins may contribute to the. (lu.se)
  • PIWI-interacting RNAs (piRNAs) are another subgroup of small non-coding RNAs emerging as key players in different biological activities, including regulating gene and protein expression, epigenetic silencing of transposable elements, and controlling the spermatogenesis by interacting with PIWI proteins. (jcimcr.org)
  • Antioxidant genes such as ferritin are transcriptionally activated in oxidative stress via the antioxidant responsive element (ARE), to which nuclear factor-E2-related factor 2 (Nrf2) binds and activates transcription. (embl.de)
  • DEAF-1 recognizes several TTCG motifs within the portion of the Dfd autoregulatory region termed 'module E.' In addition, DEAF-1 binds several similar motifs within a Dfd response element ( DRE ) from the 1.28 gene that enhances maxillary gene expression during embryogenesis (Pederson, 2000). (sdbonline.org)
  • Moreover, five pairs of clinical specimens were collected to confirm the overexpression of HNRNPC and non-ectopic expression of RBM15 in tumor tissues. (aging-us.com)
  • Human pre-mRNA splicing involves dynamic stepwise reactions in a huge protein-RNA complex called "spliceosome," which includes five kinds of small nuclear ribonucleoproteins, called U snRNPs, and many protein factors. (innovitaresearch.com)
  • GEMIN5 is a component of small nuclear ribonucleoprotein (snRNP) complexes and helps in the assembly of the spliceosome complexes. (ox.ac.uk)
  • In neurons, regulation of gene expression occurs in part through translational control at the synapse. (immuquest.com)
  • Alternative splicing of pre-mRNA is a post-transcriptional modification essential for the regulation of gene expression and function. (biomedcentral.com)
  • The hereditary ataxias are a heterogenous group of disorders with an increasing number of causative genes being described. (ox.ac.uk)
  • HSP, also known as familial spastic paraplegias or Strumpell-Lorrain disease, comprises a clinically and genetically heterogeneous group of hereditary disorders characterized by slowly progressive spastic paraparesis. (medscape.com)
  • TDP-43 is a highly conserved, ubiquitously expressed, multifunctional nucleic acid-binding protein composed of two RNA recognition motifs (RRM), nuclear localization (NLS) and export signals (NES), and a carboxy-terminal glycine rich region. (nature.com)
  • Arsenic exposure of human HaCaT keratinocytes induced nuclear accumulation of PRMT1 and PRMT4, histone H4R3 and H3R17 methylation proximal to the ARE, but not to the non-ARE regions of ferritin genes. (embl.de)
  • DEAF-1 activates the expression of Mtk and Drs promoter-luciferase fusion genes in S2 cells. (sdbonline.org)
  • Impaired control of Interferon-Responding Genes (IRGs) expression by their regulatory mechanisms, including Interferon Regulatory Factors (IRFs), may underlie these findings and it may explain the heterogeneity observed among these conditions. (frontiersin.org)
  • Signaling through the type I IFN pathway leads to an increased expression of several IFN-responding genes (IRGs). (frontiersin.org)
  • The candidate genes exhibit expression patterns in lung and heart similar to that of known PAH risk genes, and most variants occur in conserved protein domains. (biomedcentral.com)
  • Three differential expression genes (DEGs) and five prognostic m6A genes were screened out. (aging-us.com)
  • By performing unbiased analyses of the m1A, m5C, m6A and m7G methylation of mRNA, we found that within the whole brain transcriptome, with the increase of the gene expression levels, the overall level of each of these four modifications on the related genes was also progressively increased. (biomedcentral.com)
  • However, it is still unknown whether hypoxia could affect the abundance of methylation modifications on mRNAs, which further affects the expression levels of these genes. (biomedcentral.com)
  • In humans, C20orf202 has moderate mRNA abundance across cells types, though higher than average expression in the kidney and heart, it is not significantly so. (wikipedia.org)
  • To analyze splicing activity, they selected HNRNPH1 pre-mRNA (heterogeneous nuclear ribonucleoprotein H1) including a 56-nt short intron. (innovitaresearch.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • 5FMC is a nuclear complex that can only be recruited by Chtop when the latter is arginine-methylated by Prmt1. (embl.de)
  • More recently, a complex group of different neurologic and systemic compromise has also been recognized. (medscape.com)
  • Each segment is wrapped by a nucleoprotein (NP) into a viral ribonucleoprotein (RNP) complex along with a viral RNA polymerase complex comprising polymerase basic 1 (PB1) polymerase, cap‐binding PB2 subunit, and polymerase acidic (PA) endonuclease. (biomedcentral.com)
  • The differential expression of IRF4 in RA was then confirmed in publicly available gene expression datasets. (frontiersin.org)
  • The differential expression of IRF4 and IRGs observed in SLE and RA can delineate gene expression signatures associated with clinical features and treatment outcomes. (frontiersin.org)
  • Fig. 5: Proteome-wide motif search predicts indisulam selectivity confirmed by expression proteomics. (nature.com)
  • For this reason, mitochondrial carrier gene expression needs tight regulation. (cancerindex.org)
  • Protein expression and function of organic anion transporters in short-term and long-term cultures of Huh7 human hepatoma cells. (cancerindex.org)
  • GBM originate from poorly differentiated glial cells and have the characteristics of nuclear atypia, cellular polymorphism, and a high degree of mitotic activity. (biomedcentral.com)
  • Additionally C20orf202 expression increases during fetal development of kidney, lung, and intestinal tissues. (wikipedia.org)
  • At E13, fetal brains were collected from 5 dams from each group, weighed, and frozen for LC-MS/MS procedure. (biomedcentral.com)
  • To further confirm that SPF45 is a common splicing factor for a group of short introns, they performed whole-transcriptome sequencing with RNA prepared from the SPF45-knockdown cells. (innovitaresearch.com)
  • Importantly, STAT5A promoted HNRNPKP2 expression after knockdown DC-SIGNR. (biomedcentral.com)
  • DC-SIGNR promoted gastric cancer liver metastasis mediated with HNRNPKP2 which expression was regulated by STAT5A. (biomedcentral.com)
  • High m6A risk not only can suppress immunotherapy-related biological processes, but also repress the expressions of immune-checkpoint markers. (aging-us.com)
  • In terms of gene expression, microarray and RNA sequencing have been applied to determine the most affected biological processes and molecular pathways at various disease stages. (biomedcentral.com)
  • Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
  • In latest years, our group was leading in showing the high immunogenicity of MSI-induced frameshift-peptides (FSP) by determining many epitopes regarded by Testosterone levels cells [7]C[10]. (immune-source.com)
  • Here, AMSP-30 m inhibited proliferation and induced apoptosis in hypoxia-induced RA FLS (MH7A cell line), as evidenced by decreased cell viability, reduced Ki67-positive cells, G0/G1 phase arrest, lowered C-myc and Cyclin D1 protein levels, emergence of apoptotic nuclear fragmentation, raised apoptosis rates, and activation of caspase 3. (bvsalud.org)
  • Deletion of HP1β, but not HP1α, in ESCs provokes a loss of the morphological and proliferative characteristics of embryonic pluripotent cells, reduces expression of pluripotency factors and causes aberrant differentiation. (biomedcentral.com)
  • Similarly, hepatic sinusoidal endothelial E-selectin expression is up regulated by highly metastatic cells entering the liver [ 10 ]. (biomedcentral.com)
  • presents an opportunity to manipulate gene expression within the cells to treat various diseases, and acts as a powerful tool for studying gene function utilizing antisense agents to manage the diseases by regulating the expression of the specific factor that actually causes the particular disease. (genomicglossaries.com)
  • AF cells (AFCs) represent a heterogeneous population composed of three groups of adherent cells, categorized based on their morphological, proliferative and biochemical characteristics: epithelioid (E-type), amniotic fluid (AF-type) and fibroblastic (F-type) cells[2]. (123dok.org)
  • In addition, using invitro methylation assay we identified 4 new substrates for PRMT6, extending the involvement of this enzyme to other cellular processes beyond its well-established role in gene expression regulation. (embl.de)
  • These arrays contain oligonucleotide probes that span exon-exon junctions, and probes positioned within exons to determine individual exon levels and overall transcript expression. (biomedcentral.com)
  • The clones expressing the highest levels of transcript, were analyzed by Immunofluorescence imaging and Nuclear/cytoplasmic fractionation in order to evaluate hTERT subcellular localization. (123dok.org)
  • Then we evidenced that in AFCs with the higher transcript levels, the hTERT protein is present in the nuclear and cytoplasmic compartment. (123dok.org)
  • C20orf202 orthologs can be found in major groups such as mammals, reptiles, amphibians, and distantly fish. (wikipedia.org)
  • It has been found that the effect of m6A modification on RNA metabolism involves processing, nuclear export, translation and even decay. (biomedcentral.com)
  • Microscopy, biochemical fractionation and chromatin immunoprecipitation reveal a diffuse nucleoplasmic distribution, weak association with chromatin and high expression levels for HP1β in ESCs. (biomedcentral.com)
  • Through integrated analysis of single cell chromatin landscapes and gene expression states, coupled with spatial transcriptomic profiling, we are able to impute fibroblast epigenomes with temporospatial resolution. (stanford.edu)
  • In the present study, 77 ACC samples from TCGA database were divided into localized ( n = 46) and metastatic ( n = 31) groups. (aging-us.com)
  • Type I IFN production is tightly controlled at the gene expression level in a highly ordered process regulated by multiple transcription factors ( 14 ). (frontiersin.org)
  • Recent research aimed mainly at a better understanding of the genetic risk factors and modifiers associated with the onset and phenotypic expression of the sporadic disease: Genome-Wide Association Studies (GWAS) of large sCJD cohorts confirmed the significant association with PRNP codon 129, the strongest genetic risk factor [ 10 ]. (biomedcentral.com)
  • Finally, knocking out endogenous Gemin5 in mice caused early embryonic lethality, suggesting that Gemin5 expression is crucial for normal development. (ox.ac.uk)
  • Recently, with the help of next-generation sequencing, abnormal piRNA expression has been observed in different types of cancer, such as colorectal cancer (CRC). (jcimcr.org)
  • Other sterol variants with a 3β-hydroxyl group or with an aliphatic side chain did not restore density or infectivity. (microbiologyresearch.org)
  • DNA target sequencing identified multiple potential genetic contributors to the disease onset and phenotype, both in terms of coding, damaging-predicted variants, and enriched groups of SNPs in the whole cohort and the two subtypes. (biomedcentral.com)
  • Cluster analysis identified 3 gene expression signatures on the basis of IRF4 and IRGs expression which were differentially used by SLE and RA patients. (frontiersin.org)
  • Conclusions: Our study demonstrates for thefirst time the expression of the full protein hTERT by AFCs and its release outside the cell mediated by EVs, indicating a new extra telomeric role for this protein. (123dok.org)
  • Enthrallingly, HTLV-1 Tax expression also targets the functions of the centrosome, and this event is correlated with centrosome amplification, aneuploidy and transformation. (biomedcentral.com)
  • Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
  • M sequence splice site analysis revealed an evolutionarily conserved single nucleotide variant C55T in H3N2, which impaired M2 expression and was accompanied by collinear M1 and mRNA3 production. (biomedcentral.com)
  • We show that manipulation of TDP-43 expression levels causes significant defects in dendritic branching and outgrowth, without an immediate effect on cell viability. (nature.com)
  • A biological process that is crucial for gene expression in most animal life, including humans. (genomicglossaries.com)
  • In humans, this gene encodes for a nuclear protein that is primarily expressed in the lung and placenta. (wikipedia.org)
  • it has been shown that high piRNA expression may be relevant for cancer tumorigenesis and cancer prognosis in various types of human tumors [15-17]. (jcimcr.org)
  • In view of this, we conducted afirst investigation on the expression of human hTERT gene known to be among the key triggers of organ regeneration in AFCs and in the EVs they secrete. (123dok.org)
  • Phosphatidylcholine (PC), phosphatidate (PA), and 1-acylglycerophosphocholine (lysoPC) were decreased in liver and serum samples of the db/db group, while PC and lysoPC were decreased in kidney and perinephric fat samples. (bvsalud.org)
  • Manipulation DC-SIGNR expression in BGC823 and SGC7901 cell lines was mediated by lentivirus. (biomedcentral.com)
  • In the present study we aimed to evaluate the associations between IRF4 gene expression and those of IRGs in SLE and RA patients to gain insight about its links with the IFN signature as well as to explore the potential clinical relevance of these associations. (frontiersin.org)
  • TNFα-blockade led to changes in the association between IRF4 and IRGs, whereas increasing IRF4 expression was associated with a good clinical outcome in RA. (frontiersin.org)
  • DC-SIGNR serum level was significantly increased in gastric cancer patients compared with healthy group. (biomedcentral.com)
  • Splicing of the NS segment, which encodes the host shutoff protein non-structural protein 1 (NS1), generates the nuclear export protein (NEP/NS2) that is involved in viral RNP export from the host nucleus [ 8 ]. (biomedcentral.com)
  • HNRNPCL1 (Heterogeneous Nuclear Ribonucleoprotein C Like 1) has a role in RNA binding and nucleosome assembly. (wikipedia.org)
  • There was a clear difference in the appearance between both groups, in that the LPS-treated mice were clearly dead with no respiratory effort, whereas the LPS/Pyl A-treated mice were pink, moved spontaneously or with stimulus, and had respiratory effort. (mdmsignaling.com)
  • The interplay between mechanical strain and inflammation in pulmonary fibrosis remain incompletely understood.APPROACH: In this study, we used conditional KO mice to block mechanotransduction by knocking out FAK (Focal Adhesion Kinase) expression in fibroblasts, followed by induction of pulmonary fibrosis using bleomycin. (stanford.edu)
  • Indoleacetaldehyde (IAld) and 5-hydroxy-l-tryptophan (5-HTP) in kidney samples, and serotonin (5-HT) in fecal samples were increased in the db/db group. (bvsalud.org)
  • Although at 250 μg Pyl A alone did not induce labour, at 500 μg labour was induced at 44·8 hr post injection from 64·6 hr in the vehicle control group. (mdmsignaling.com)
  • Classic evolutionary theories only consider the functional consequence of missense mutations, and the synonymous mutations are regarded as neutral sites and are used as "control group" to test the selection force on missense mutations [ 3 , 4 , 5 ]. (biomedcentral.com)