PeroxidaseNADPHSuperoxide dismutaseCytochromePolyphenol oxidaseAntioxidantPeroxidasesAlpha-tocopherolEnzymesInduces expressionGene expressionExogenousRedoxEnzymeAccumulationAmidationCopperTransgenicAscorbicEndogenousEffluxProtoplastsProteinsMoleculesActivityBeta-cateninProteinMembraneMechanismRegulationEthyleneExtracellularAdditionHumanOperateCellsRoleIronPlant
Peroxidase7
- During an invasion, E. guineensis roots stockpile salicylic acid, which is a signal to downregulate its own expression of ascorbate oxidase and ascorbate peroxidase. (wikipedia.org)
- Furthermore, the activities of antioxidative enzymes catalase (CAT) and ascorbate peroxidase (APX) were increased by ethylene + H 2 S treatment in comparison with ethylene alone, whereas the activities of lipoxygenase (LOX) and polyphenol oxidase (PPO) were attenuated by H 2 S treatment. (ashs.org)
- Transient expression and RNA interference silencing of ZmMAP65-1a in mesophyll protoplasts further revealed that ZmMAP65-1a is required for the BR-induced increase in expression and activity of superoxide dismutase (SOD) and ascorbate peroxidase (APX). (edu.hk)
- Calcium and EGTA application led to oxidative stress alleviation as evidenced by H 2 O 2 content decrease and the restoration of catalase and ascorbate peroxidase activities at a level similar to control roots. (springer.com)
- an oxidative burst triggered by the treatment of Arabidopsis plants with a cell wall elicitor prepared from the fungus Fusarium oxysporum could be abolished by knocking down the transcript levels of PRX33 and PRX34 through the transgenic expression of a French bean class III peroxidase cDNA (FBP1) in an antisense orientation (Daudi et al. (rehabsociety.org.hk)
- In latest several years, considerable proof has gathered suggesting that physiologically relevant concentrations of SA remedy can boost H2O2 levels, and H2O2 has been proposed as currently being functionally downstream of SA in plants dependent on the proof that SA can participate in regulating the antioxidant enzymes, these kinds of as catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX) [61,78,91-94]. (idhinhibitor.com)
- Cu/Zn superoxide dismutase, glutathione reductase and ascorbate peroxidase levels during drought stress in potato. (scielo.org.za)
NADPH6
- iNOS expression requires NADPH oxidase-dependent redox signaling in microvascular endothelial cells. (umaryland.edu)
- Conversely, transient expression of ZmMAP65-1a in maize mesophyll protoplasts enhanced BR-induced H 2 O 2 accumulation, while transient silencing of ZmMAP65-1a blocked the BR-induced expression of NADPH oxidase genes and inhibited BR-induced H 2 O 2 accumulation. (edu.hk)
- Furthermore, ZmMAP65-1a functions in H 2 O 2 self-propagation via regulation of the expression of NADPH oxidase genes in BR signalling. (edu.hk)
- 2011. NADPH oxidase overexpression in human colon cancers and rat colon tumors induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP). . (oregonstate.edu)
- Prolonged exposure to LPS increases iron, heme, and p22phox levels and NADPH oxidase activity in human aortic endothelial cells: inhibition by desferrioxamine. (oregonstate.edu)
- DPI, a distinct inhibitor of membrane-joined NADPH oxidase, which is one particular of the principal resources of H2O2 development in plant cells, inhibited NADPH oxidase activity and also inhibited ARF in mung bean hypocotyls. (idhinhibitor.com)
Superoxide dismutase4
- Copper-dependent metalloenzymes relevant to the clinical phenotype include tyrosinase (pigmentation of skin and hair), lysyl oxidase (elastin and collagen cross-linking), ascorbate oxidase (skeletal development), monoamine oxidase (possibly responsible for pili torti), superoxide dismutase (free-radical detoxification), dopamine beta-hydroxylase (catecholamine production), peptidyl-glycine alpha-amidating mono-oxygenase (bioactivation of peptide hormones), and cytochrome c oxidase (electron transport and possibly responsible for hypothermia). (medscape.com)
- ATOX1 for instance, delivers copper to the ATPases, CCS distributes copper to Cu/Zn superoxide dismutase (SOD1), COX17 delivers the copper to the cytochrome c oxidase in the mitochondria, and MURR1 is implicated in the lysosomal storage of copper as well as the excretion into bile. (vin.com)
- Increased resistance to oxidase stress in transgenic plants that overexpress chloroplastic Cu/Zn superoxide dismutase. (scielo.org.za)
- cDNA Cloning and expression of the plastidic copper/zink - superoxide dismutase from spinach (Spinacea vleracea L.) leaves. (scielo.org.za)
Cytochrome1
- Cytochrome c oxidase (complex IV) catalyses the reduction of molecular oxygen to water and ATP synthase (complex V) generates ATP from ADP. (bmj.com)
Polyphenol oxidase3
- Defensive role of tomato Polyphenol oxidase against cotton bollworm ( Helicoverpa armigera ) and Beet armyworm ( Spodoptera exigua ). (indianentomology.org)
- System in activates synthesis of wound-inducible tomato leaf polyphenol oxidase via the octadecanoid defense signaling pathways. (indianentomology.org)
- A survey of wound- and methyl jasmonate-induced leaf polyphenol oxidase in crop plants. (indianentomology.org)
Antioxidant2
- Oxidative stress and free radical production have been implicated in Alzheimer's disease, where low levels of the antioxidant vitamin C (ascorbate) have been shown to be associated with the disease. (ox.ac.uk)
- Likewise, transient expression of ZmMPK5 enhanced BR-induced activities of the antioxidant defence enzymes SOD and APX in a ZmMAP65-1a-dependent manner. (edu.hk)
Peroxidases1
- Moreover, this study has elucidated that SA‐induced stomatal closure involves extracellular oxidative burst (Figs 2 & 3) and that the extracellular oxidative burst is mediated with SHAM‐sensitive peroxidases but not NAD(P)H oxidases (Figs 1d & 2). (rehabsociety.org.hk)
Alpha-tocopherol2
- Non-enzymatic defenses are exerted by molecules such as alpha-tocopherol, betacarotene, ascorbate, and a ubiquitous low molecular thiol component, Glutathione. (vin.com)
- Potential antiatherogenic mechanisms of ascorbate (vitamin C) and alpha-tocopherol (vitamin E). . Circ Res. (oregonstate.edu)
Enzymes2
- Moreover, the analysis of the transcriptional system relating to the up-cited enzymes revealed a decreased gene expression subsequent to the enrichment of germination medium with the effectors. (springer.com)
- However, deleting sspA had no effect on uric acid or purine utilization, as we hypothesized, a result also in line with the observation that expression of SspA was not affected by regulatory mutants and conditions known to control purine catabolic enzymes. (uoa.gr)
Induces expression2
- The presence of ascorbate induces expression of brain derived neurotrophic factor in SH-SY5Y neuroblastoma cells after peroxide insult, which is associated with increased survival. (ox.ac.uk)
- Temperature affects invertebrate hormone system, and the increased temperature induces expression of endocrine signaling genes of chironomids Chironomus riparius [ 14 ]. (biomedcentral.com)
Gene expression4
- One important strategy for studying these dynamic but highly regulated host defenses is to observe gene expression patterns in the host 5 . (nature.com)
- Inhibiting the activity and gene expression of ZmMPK5 significantly prevented the BR-induced expression of ZmMAP65-1a. (edu.hk)
- gene expression. (meatrition.com)
- The response of plants to anaerobiosis is characterized by a dramatic change in gene expression. (ristudypost.com)
Exogenous4
- Exogenous copper and iron stimulate the ascorbate-induced [Ca2+]cyt. (deepdyve.com)
- Exogenous ascorbate also induces a moderate increase in programmed cell death symptoms in intact roots, but it does not activate Ca2+ influx currents in patch-clamped root protoplasts. (deepdyve.com)
- Both exogenous and BR-induced endogenous H 2 O 2 increased the expression of ZmMAP65-1a. (edu.hk)
- Both exogenous and BR-induced endogenous H2O2 increased the expression of ZmMAP65-1a. (edu.hk)
Redox2
- These data show that apoplastic redox-active transition metals are involved in the ascorbate-induced [Ca2+]cyt. (deepdyve.com)
- This mechanism may underlie ascorbate release, signalling phenomena, apoplastic redox reactions, iron acquisition, and control the ionic and electrical equilibrium (together with K+ efflux via GORK channels). (deepdyve.com)
Enzyme5
- Primate lineage lost ability to synthesize Vitamin C from glucose 66 million years ago with loss of l-gulonolactone oxidase enzyme. (meatrition.com)
- The single enzyme responsible for this reaction is peptidylglycine α-amidating monooxygenase (PAM: EC 1.14.17.3), a copper- and ascorbate-dependent Type I membrane protein. (biomedcentral.com)
- Laccase (EC 1.10.3.2 ) (urishiol oxidase), an enzyme found in fungi and plants, which oxidizes many different types of phenols and diamines. (expasy.org)
- L-ascorbate oxidase (EC 1.10.3.3 ), a higher plant enzyme. (expasy.org)
- Cofactor proteins in the assembly and expression of blood clotting enzyme complexes. (expasy.org)
Accumulation3
- EPR spectroscopy measurements demonstrate that salinity (NaCl) triggers the accumulation of root apoplastic ascorbyl radicals in an A9C-dependent manner, confirming that l-ascorbate leaks through anion channels under depolarization. (deepdyve.com)
- Mi Y L, Pulla R K, Park J M, et al.Over-expression of L-gulono-γ-lactone oxidase (GLOase) gene leads to ascorbate accumulation with enhanced abiotic stress tolerance in tomato[J]. In Vitro Cellular & Developmental Biology - Plant, 2012, 48(5): 453-461. (tea-science.com)
- Alterations in the expression of genes involved in gibberellin (GA) biosynthesis and inactivation results in increased and decreased biomass accumulation, respectively, which is positively correlated with the photosynthetic rates at the whole plant level [ 8 ]. (biomedcentral.com)
Amidation1
- Amidation was inhibited by copper chelation, but was not enhanced by the addition of additional copper or ascorbate. (biomedcentral.com)
Copper2
- Structurally ceruloplasmin exhibits internal sequence homology, and seem to have evolved from the triplication of a copper-binding domain similar to that found in laccase and ascorbate oxidase. (expasy.org)
- Both patterns are derived from the same region, which in ascorbate oxidase, laccase, in the third domain of ceruloplasmin, and in copA, contains five residues that are known to be involved in the binding of copper centers. (expasy.org)
Transgenic2
- In rice, studies on miR159 were either based upon genome-wide expression analyses focused upon responses to different nitrogen forms and abiotic stress or upon phenotypic studies of transgenic plants overexpressing its precursor. (biomedcentral.com)
- Further analysis from the RNA-seq data showed that the decreased cell divisions in STTM159 transgenic plants may result, at least partly from the lower expression of the genes involved in cell cycle and hormone homeostasis, which provides new insights of rice miR159-specific functions. (biomedcentral.com)
Ascorbic1
- 1997. NF-kappa B-independent suppression of HIV expression by ascorbic acid. . (oregonstate.edu)
Endogenous1
- However, endogenous BR positively regulated the expression and activity of fructose-1,6-bisphosphatase. (biomedcentral.com)
Efflux1
- Intriguingly, the replacement of gluconate with ascorbate in the patch-clamp pipette reveals a large ascorbate efflux current, which shows sensitivity to the anion channel blocker, anthracene-9-carboxylic acid (A9C), indicative of the ascorbate release via anion channels. (deepdyve.com)
Protoplasts1
- Treatment with BR increased the expression of ZmMAP65-1a in maize (Zea mays) leaves and mesophyll protoplasts. (edu.hk)
Proteins3
- Proteins, extracted from SH-SY5Y cells that were treated with either ascorbate or peroxide alone or with ascorbate prior to peroxide, were separated by two-dimensional gel electrophoresis and analyzed for oxidation. (ox.ac.uk)
- Co-incubation for 24 hours decreased the number of oxidised proteins (e.g. acyl CoA oxidase 3) and induced brain derived neurotrophic factor (BDNF) expression. (ox.ac.uk)
- In an in silico search for correlated gene loss with fungal peroxisomal uric acid oxidase (UOX), we identified PMP22-like proteins, some of which function as promiscuous channels in organellar membranes. (uoa.gr)
Molecules1
- In water solutions, this substance is present as a monovalent anion, ascorbate- (Asc-), which easily donates electrons to oxidized molecules and scavenges reactive oxygen species (ROS). (deepdyve.com)
Activity1
- Garchery C, Gest N, Do P T, et al.A diminution in ascorbate oxidase activity affects carbon allocation and improves yield in tomato under water deficit[J]. Plant Cell & Environment, 2013, 36(1): 159-175. (tea-science.com)
Beta-catenin2
- 2003. Mutational analysis of Ctnnb1 and Apc in tumors from rats given 1,2-dimethylhydrazine or 2-amino-3-methylimidazo[4,5-f]quinoline: mutational 'hotspots' and the relative expression of beta-catenin and c-jun. . (oregonstate.edu)
- 2008. Protective versus promotional effects of white tea and caffeine on PhIP-induced tumorigenesis and beta-catenin expression in the rat. . (oregonstate.edu)
Protein2
- Although the heat-acclimated and unacclimated larvae upregulated expression of heat shock protein genes under heat stress including HSP70 , HSP27 and CRYAB , their biosynthesis, metabolism and detoxification-related genes expressed differentially. (biomedcentral.com)
- Moreover, other research have indicated that H2O2 does not operate downstream of SA in regulating PR protein expression [ninety nine]. (idhinhibitor.com)
Membrane1
- The function of PM transporters is finely regulated at the cellular level, in response to physiological or stress signals that promote, via α-arrestin-assisted ubiquitination, their endocytosis and vacuolar/lysosomal degradation, and in some cases recycling to the PM. Importantly, transporter oligomerization and specific interactions with membrane lipids are emerging as important players in transporter expression, function and turnover. (uoa.gr)
Mechanism1
- In this study, neuroblastoma SH-SY5Y cells were treated with hydrogen peroxide in the presence of ascorbate in order to elucidate the mechanism(s) of protection against oxidative stress afforded by ascorbate. (ox.ac.uk)
Regulation1
- Intestinal microbiota transplantation reveals the role of microbiota in dietary regulation of RegIIIβ and RegIIIγ expression in mouse intestine. (shengsci.com)
Ethylene1
- Further investigations showed that H 2 S repressed the expression of ethylene synthesis-related genes AdSAM , AdACS1 , AdACS2 , AdACO2 , and AdACO3 and cysteine protease genes, such as AdCP1 and AdCP3 . (ashs.org)
Extracellular1
- The expression of tenascin (Tn), an extracellular matrix glycoprotein, was analyzed in the human small intestine. (shengsci.com)
Addition1
- In addition, the expression trends of CsAO and CsAPX in both cultivars were similar under NaCl (200βmmol·L -1 ) treatment, but the variation ranges were different, which might be related to the different stress response in tea plant. (tea-science.com)
Human1
- Expression of tenascin in the developing human small intestine. (shengsci.com)
Operate1
- The ascorbate-glutathione cycle (Halliwell-Asada pathway) may operate to reduce hydrogen peroxide (H2O2). (ristudypost.com)
Cells6
- SH-SY5Y cells treated with ascorbate prior to co-incubation with peroxide showed increased viability in comparison to cells treated with peroxide alone. (ox.ac.uk)
- Enhanced expression of BDNF may contribute to the protective effects of ascorbate against oxidative stress in neuronal cells. (ox.ac.uk)
- 2002. Myeloperoxidase-dependent caspase-3 activation and apoptosis in HL-60 cells: protection by the antioxidants ascorbate and (dihydro)lipoic acid. . (oregonstate.edu)
- BMP-2 stimulated the expression of Sox9, Col2a1, and Aggrecan mRNAs in free-body and synovial cells and that of Runx2, Col1a1, and Osteocalcin mRNAs in the synovial [corrected] cells only. (shengsci.com)
- Noggin suppressed the expression of Sox9, Col2a1, Aggrecan, and Runx2 mRNAs in both the free-body and synovial cells. (shengsci.com)
- Further, it inhibited Osteocalcin expression in the synovial cells. (shengsci.com)
Role1
- Potential Role of SLC5A8 Expression in the Etiology of Subacute Ruminal Acidosis. (oregonstate.edu)
Iron1
- The FET3 gene of S. cerevisiae encodes a multicopper oxidase required for ferrous iron uptake. (expasy.org)
Plant2
- Altering expression of miRNAs can result in multiple visible phenotypes of a plant. (biomedcentral.com)
- Expression of miR159 is abundant and widespread in all plant parts. (biomedcentral.com)