• A novel point mutation in CD18 causing leukocyte adhesion deficiency in a Chinese patient. (lu.se)
  • Two Novel Frame Shift, Recurrent and De Novo Mutations in the ITGB2 (CD18) Gene Causing Leukocyte Adhesion Deficiency in a Highly Inbred North African Population. (lu.se)
  • ICAM-1 is an 85 kDa type I transmembrane glycoprotein that is encoded by Icam1 (Intercellular adhesion molecule 1). (bdbiosciences.com)
  • After two, four and 24 hours, fluorescence activated cell sorter (FACS) analysis for vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and E-selectin was performed. (biomedcentral.com)
  • Mechanistic studies revealed that this progression was mediated by suppressed TNFα levels, both locally and systemically, resulting in reduced expression of tumor endothelial adhesion molecules, particularly intercellular adhesion molecule-1 (ICAM-1) and a subsequent decrease in the number of activated and effector CD8 + T cells in the tumor. (aacrjournals.org)
  • Since leucocyte-endothelial cell interactions involve various adhesion receptors we have analysed the expression and distribution pattern of the neural cell adhesion molecule (NCAM), the intercellular adhesion molecule-1 (ICAM-1), the vascular cell adhesion molecule-1 (VCAM-1), the endothelial leucocyte adhesion molecule-1 (ELAM-1), and the cluster of differentiation antigen-44 (CD44) on flat preparations of normal and organ-cultured HCECs. (bmj.com)
  • Polysialic acid (polySia) is a large glycan with restricted expression, typically found attached to the protein scaffold neural cell adhesion molecule (NCAM). (ca.gov)
  • In this immunohistochemical light-microscopic study we analyzed the expression pattern of adhesion molecules in normal and pathological human corneas (cases of corneal inflammation and degenerative disorders). (nih.gov)
  • The data suggest that diabetic mice present impaired immunological response to Pb18 infection and insulin modulates inflammation by reducing IL-6 levels in lung and CINC-1 levels in spleen and liver homogenates, restoring leukocyte concentrations in PeLF and also restoring populations of dendritic cells and B lymphocytes in lungs of diabetic mice, permitting the host to better control the infection. (hindawi.com)
  • CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation. (biolegend.com)
  • We suggest that modulation of ELAM-1, VCAM-1, and CD44 expression on HCECs may contribute to the regulation of leucocytes-HCECs interaction in the case of anterior segment inflammation. (bmj.com)
  • This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. (southernbiotech.com)
  • Rabbit vascular endothelial adhesion molecules: ELAM-1 is most elevated in acute inflammation, whereas VCAM-1 and ICAM-1 predominate in chronic inflammation. (cdc.gov)
  • Therefore, the aim of the present work was to evaluate the effect of NAC on interleukin-8 (IL-8) and matrix metalloproteinase-9 (MMP-9) production as well as intercellular cell adhesion molecule-1 (ICAM-1) expression by BAL cells from interstitial lung diseases. (nih.gov)
  • Production of IL-8 and MMP-9 was evaluated by specific enzyme-linked immuno-sorbent assays and ICAM-1 expression was studied by immunohistochemistry. (nih.gov)
  • NAC exerted a dose-dependent inhibitory effect on IL-8 and MMP-9 release and ICAM- expression by BAL macrophages and lymphocytes from patients with IPF and sarcoidosis. (nih.gov)
  • The analyzed molecules included the beta 1 integrin or VLA family VLA-1-6, the beta 2 integrins or leukocyte integrins LFA-1, Mac-1, and p150,95, the immunoglobulins LFA-3, CD2, ICAM-1 and VCAM-1 and the selectins ELAM-1 and GMP-140. (nih.gov)
  • On corneal epithelium, increased expression of the alpha 2 subunit of VLA-2 was detected and ICAM-1 was induced on the basal epithelial cells. (nih.gov)
  • On corneal stromal keratocytes, LFA-3 was induced and expression of the alpha subunits of VLA-1-6 and ICAM-1 was increased. (nih.gov)
  • 1A29 mAb inhibits Phorbol 12-Myristate 13-Acetate (PMA)-induced aggregation of phytohemagglutinin (PHA)-stimulated splenic blasts, as well as the adhesion of mitogen-stimulated blasts to high endothelial venule (HEV) cells and purified ICAM-1. (bdbiosciences.com)
  • In addition, the expression of ICAM-1 in lung parenchyma in areas outside the granulomas of OVA-orally tolerant mice was significantly reduced. (hindawi.com)
  • Furthermore, clarified culture supernatants recovered from C- Fe-treated macrophages raise vascular endothelial cells to an activated state as indicated by the induction of endothelial- leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin. (cdc.gov)
  • Expression of ICAM-1, VCAM-1, and E-selectin was not altered following incubation with tryptase, but the potent granulocyte chemoattractant IL-8 was released in a dose-dependent fashion in response to physiologically relevant concentrations, with maximal levels in supernatants after 24 h. (eurekamag.com)
  • Inhibition of IκBα phosphorylation and degradation results in the inactivation of NF-κB and NF-κB-dependent expression of endothelial-leukocyte adhesion molecules, E-selectin, VCAM-1 and ICAM-1. (enzolifesciences.com)
  • Adhesion molecules are cell surface receptors that are probably important in various cell-cell and cell-extracellular matrix interactions of the cornea. (nih.gov)
  • Corresponding receptors on leukocytes probably enable a selective recruitment of different leukocyte populations in inflammatory corneal diseases. (nih.gov)
  • LECs can modulate dendritic cell function, present antigens to T cells on MHC class I and MHC class II molecules, and express immunomodulatory cytokines and receptors, which suggests that their roles in adaptive immunity are far more extensive than previously realized. (jci.org)
  • Glucocorticoids bind steroid receptors in the cytoplasm, alter DNA expression and inhibit formation of inflammatory mediators such as interleukin-6 (IL-6), IL-8, prostaglandins, and vascular endothelial growth factor (VEGF). (dovepress.com)
  • These autoantibodies then bind neutrophils, subsequently leading to neutrophil adhesion to vessel walls and cellular activation. (medscape.com)
  • Leukocyte rolling in postcapillary venules of inflamed tissues is reduced in L-selectin-deficient mice and mice treated with L-selectin blocking antibodies, but the glycoprotein ligand for L-selectin in inflamed venules is unknown. (rupress.org)
  • Characterization of four CD18 mutants in leucocyte adhesion deficient (LAD) patients with differential capacities to support expression and function of the CD11/CD18 integrins LFA-1, Mac-1 and p150,95. (lu.se)
  • The extravasation of leukocytes from the vascular bed into surrounding tissues and organs is part of the host defense mechanisms against invading pathogens. (biomedcentral.com)
  • The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling. (southernbiotech.com)
  • The principal objective of our research is to investigate the mode, dynamics and mechanisms of leukocyte transmigration, the final stage in the leukocyte adhesion cascade, that describes the movement of leukocytes from the vascular lumen into inflamed and/or injured tissues. (qmul.ac.uk)
  • This work uses a model of experimental T1DM to investigate the intervention of insulin in a model of murine PCM induced by Paracoccidioides brasiliensis , analyzing the process of cell migration (adhesion molecules expression, leukocyte phenotyping), molecular mechanisms (production and secretion of cytokines), intracellular mechanisms (signaling pathways) and phagocytic and microbicidal activities in alveolar macrophages. (usp.br)
  • The decreased expression of adhesion molecules in corneal degenerative disorders is probably a sign of reduced cell-cell and cell-extracellular matrix interactions. (nih.gov)
  • In situ immunohistochemical analysis of cell adhesion molecules on human corneal endothelial cells. (bmj.com)
  • After 55 days, infected diabetic mice exhibited fewer leukocytes in both peritoneal lavage fluid (PeLF) and bronchoalveolar lavage fluid and reduced secretion of interleukin- (IL-) 6 in lungs. (hindawi.com)
  • Some experiments prove that aggressive pollutants of tobacco smoke (benzopyrene, peroxynitrite, acrolein, cyanides, peroxides, etc.) can cause direct damage to endothelial cells due to expression of adhesion molecules on their surface and intensification of lipid peroxidation. (intechopen.com)
  • In the Ingenuity Pathways Analysis of RNA expression data, the CDAA-HF-T(-) feeding revealed common signal changes in nontumoral and tumoral liver tissues, including increased IL-8 and RhoGTPases signaling and decreased lipid metabolism. (bvsalud.org)
  • Specifically, we found that human NK cells modulate expression of NCAM and the degree of polymerization of its polySia glycans according to activation state. (ca.gov)
  • This contrasts with the mouse, where polySia and NCAM expression are restricted to multipotent hematopoietic progenitors and cells developing along a myeloid lineage. (ca.gov)
  • Increased expression of the VLAs probably promotes cell-extracellular matrix and cell-cell interactions. (nih.gov)
  • A key approach is the application of confocal intravital microscopy that enables rigorous and direct means of investigating the interactions of leukocytes with different components of microvessel walls (endothelial cells, pericytes and the vascular basement membrane) in real-time in vivo. (qmul.ac.uk)
  • Ontogeny of lymphocyte homing receptor expression in the mouse thymus. (southernbiotech.com)
  • A novel CD18 genomic deletion in a patient with severe leucocyte adhesion deficiency: a possible CD2/lymphocyte function-associated antigen-1 functional association in humans. (lu.se)
  • Leukocyte adhesion deficiency results from an adhesion molecule defect that causes granulocyte and lymphocyte dysfunction and recurrent soft-tissue infections. (msdmanuals.com)
  • Neutrophil function and molecular analysis in severe leukocyte adhesion deficiency type I without separation delay of the umbilical cord. (lu.se)
  • The 1A29 antibody can reportedly inhibit leucocyte infiltration in in vivo systems, blocks induction of experimental allergic encephalomyelitis, and reduces NK-cell adhesion to tumor cells. (bdbiosciences.com)
  • Herein we show the expression of polySia on hematopoietic progenitor cells, and demonstrate a role for this glycan in immune response using both acute inflammatory and tumor models. (ca.gov)
  • Sialyltransferase 8Sia IV(-/-) mice, which lacked polySia expression in the immune compartment, demonstrated an increased contact hypersensitivity response and decreased control of tumor growth as compared with wild-type animals. (ca.gov)
  • Through whole-genome sequencing of single molecules of circulating cell-free DNA, we found that tumor-derived mutations in cancer genomes are associated with regions of late replication timing and other chromatin features. (cdc.gov)
  • As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. (biolegend.com)
  • High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. (biolegend.com)
  • Thus, we conclude that one mechanism by which DHEA may exert its protection in animal models is via the differential regulation of adhesion molecule expression. (biomedcentral.com)
  • This study provides a differential gene expression profile between TMJ fibrous and bony ankylosis. (medscimonit.com)
  • Similarly to other inflammatory reactions, one critical aspect of granuloma formation is leukocyte migration dependent on the expression of adhesion molecules and cytokines [ 14 , 18 , 19 ]. (hindawi.com)
  • Secondary endpoints were systemic cytokine concentrations, leukocyte counts, and the phagocytic activity of granulocytes and monocytes. (asahq.org)
  • Polysialic acid, a glycan with highly restricted expression, is found on human and murine leukocytes and modulates immune responses. (ca.gov)
  • A novel 3' splice-site mutation and a novel gross deletion in leukocyte adhesion deficiency (LAD)-1. (lu.se)
  • The effect of gentamicin-induced readthrough on a novel premature termination codon of CD18 leukocyte adhesion deficiency patients. (lu.se)
  • Characterization of 11 new cases of leukocyte adhesion deficiency type 1 with seven novel mutations in the ITGB2 gene. (lu.se)
  • ITGB2 mutation combined with deleted ring 21 chromosome in a child with leukocyte adhesion deficiency. (lu.se)
  • Reversion mutations in patients with leukocyte adhesion deficiency type-1 (LAD-1). (lu.se)
  • Functional characterization of natural killer cells in type I leukocyte adhesion deficiency. (lu.se)
  • Somatic revertant mosaicism in a patient with leukocyte adhesion deficiency type 1. (lu.se)
  • Unique CD18 mutations involving a deletion in the extracellular stalk region and a major truncation of the cytoplasmic domain in a patient with leukocyte adhesion deficiency type 1. (lu.se)
  • Chemotaxis of non-compressed blood polymorphonuclear leukocytes from an adolescent with severe leukocyte adhesion deficiency. (lu.se)
  • Genetic analysis of patients with leukocyte adhesion deficiency: genomic sequencing reveals otherwise undetectable mutations. (lu.se)
  • Nonopsonic phagocytosis of Pseudomonas aeruginoas: insights from an infant with leukocyte adhesion deficiency. (lu.se)
  • Manifestations of leukocyte adhesion deficiency usually begin in infancy. (msdmanuals.com)
  • Diagnosis of leukocyte adhesion deficiency is by detecting absence or severe deficiency of adhesive glycoproteins on the surface of WBCs using monoclonal antibodies (eg, anti-CD11, anti-CD18) and flow cytometry. (msdmanuals.com)
  • This Review summarizes the emergent evidence that LECs are important in maintaining peripheral tolerance, limiting and resolving effector T cell responses, and modulating leukocyte function. (jci.org)
  • Immunologists recognized the importance of lymphatic vessels as channels for leukocyte trafficking from peripheral sites to their draining LNs ( 4 - 6 ), and as conduits for soluble antigens that can be taken up directly by LN-resident B cells and immature DCs ( 7 - 10 ), which help regulate the kinetics of antigen presentation. (jci.org)
  • This was accompanied by higher numbers of Ly6C hi monocytes in peripheral blood, as well as higher expression of monocyte chemoattractant protein-1 and adhesion molecules in the hearts of MI-operated Hmox1 −/− mice. (springer.com)
  • We also observed host-dependent differences for interaction of platelets with C. albicans , showing enhanced platelet aggregation, adhesion and activation in murine blood. (frontiersin.org)
  • DAVID was used to perform the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis for the different expression genes (DEGs). (medscimonit.com)
  • A structurally and functionally related group of genes, lymph node homing receptor(LHR), granule membrane protein 140(GMP-140), and endothelial leukocyte adhesion molecule 1(ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. (innov-research.com)
  • Further analysis using single-cell and spatial data from psoriasis samples confirmed the expression of hub genes that had low expressions in psoriasis tissue but were up-regulated after anti-IL17A treatments. (bvsalud.org)
  • Notably, hub genes exhibited predominant expression in cluster 3 fibroblasts, which were primarily engaged in the regulation of the extracellular matrix and were predominantly located in the reticular dermis. (bvsalud.org)
  • In turn, oxidized lipoproteins in the tunica intima of the vessel work as attractants for chemotaxis of leukocytes and monocytes that start to produce pro-inflammatory cytokines in big amounts. (intechopen.com)
  • Degenerative disorders (in contrast to normal cornea): In corneas with degenerative disorders we found decreased expression of adhesion molecules. (nih.gov)
  • This is the first demonstration of polySia expression and regulation on myeloid cells, and the results in animal models suggest a role for polySia in immune regulation. (ca.gov)
  • Up-regulation of leukocyte CXCR4 expression by sulfatide: an L-selectin-dependent pathway on CD4 + T cells. (southernbiotech.com)
  • The anti-inflammatory actions of corticosteroids occur with a considerable delay (within hours or days) because of the multiple steps of cellular actions required to change protein expression. (ersjournals.com)
  • TNF-α expression after stroke stimulates expression of tissue factor and adhesion molecules for leukocytes, release of interleukin-1 (IL-1), nitric oxide, factor VIII/von Willebrand factor, platelet-activating factor and endothelin, suppression of the thrombomodulin-protein C-protein S system, reduction of tissue-plasminogen activator and release of plasminogen activator inhibitor-1. (eurekaselect.com)
  • Meanwhile, macrophage inflammatory protein 2 (MIP-2) expression levels were upregulated in nontumoral liver tissue from the end of Week 13 of CDAA-HF-T(-) feeding to the end of Week 63. (bvsalud.org)
  • BZK induced a significant increase in the severity of the clinical score, infiltration of inflammatory cells, local expression of inflammatory cytokines in subcutaneous tissue, and total serum immunoglobulin (Ig) E. PVP-I increased the clinical score, number of mast cells, and production of inflammatory cytokines, and total serum IgE. (medsci.org)
  • DESCRIPTION (provided by applicant): Therapeutic stem cell gene transfer relies on long-term gene expression achieved by integration of new DNA into the cellular genome. (sbir.gov)
  • Results: TNFα supplementation resulted in catabolic stimulation with increased levels of NO and IL-6, upregulation of catabolic gene expression, and downregulation of anabolic markers. (bvsalud.org)
  • Cell adhesion molecules are linked with the perpetuated influx of activated leukocytes into inflammatory sites. (bvsalud.org)
  • In contrast, catabolic markers and IL-8 expression were upregulated by 41.5-EVi1. (bvsalud.org)
  • Lipids, as a kind of vital metabolites, comprise the majority of cellular membranes, play important roles as signaling molecules and contribute significantly to energy [11]. (researchsquare.com)
  • To investigate whether leukocyte-expressed PSGL-1 is mediating L-selectin-dependent rolling, we reconstituted lethally irradiated wild-type mice with PSGL-1 −/− bone marrow cells. (rupress.org)
  • In this review, we discuss the changes in irradiated cancer cells and immune cells in the TME under different RT regimens and describe existing and potential molecules that could be targeted to improve the therapeutic effects of RT. (nature.com)