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  • cDNAs
  • Although more than 34,000 cDNA sequences for the axolotl Ambystoma mexicanum , 677,000 cDNAs for the African clawed frog Xenopus laevis , and 1,271,000 cDNAs for the western clawed frog Xenopus tropicalis are available [ 8 - 13 ], cDNA resources are lacking in the newt field. (molvis.org)
  • protein
  • Moreover, it allows easier association to mRNAs and protein, allow detection of splice variants plus fewer sequences to analyse ( Ioannidis, 2004 ). (ukessays.com)
  • A D. dendriticum cDNA encoding an 8-kDa recombinant protein has been cloned, expressed in Escherichia coli and evaluated in dicrocoeliosis diagnosis using both Western Blot and enzyme-linked immunosorbent assay (ELISA). (csic.es)
  • Sequences representative of particular functions or structures have been collected from public databases to curate smaller databases useful for studying protein function. (go.jp)
  • Pearson WR (1991) Identifying distantly related protein sequences. (springer.com)
  • the NCBI non-redundant (nr) protein and nucleotide sequence databases, typically updated on a daily basis with all data from GenBank, Swiss-Prot, and the PIR. (wikipedia.org)
  • Matching the in silico translated protein sequences of all 6 open reading frames to the amino acid sequence to annotated proteins, or blastx, is the preferred alignment method as it increases the likelihood of identifying a novel pathogen by matching to a related strain/species. (wikipedia.org)
  • The protein encoded by this gene is a ubiquitously expressed nuclear protein and belongs to a highly conserved subfamily of WD-repeat proteins. (wikipedia.org)
  • Members of the ZAS family, such as ZAS2 (HIVEP2), are large proteins that contain a ZAS domain, a modular protein structure consisting of a pair of C2H2 zinc fingers with an acidic-rich region and a serine/threonine-rich sequence. (wikipedia.org)
  • axolotl
  • Expressed sequence tag (EST) markers were developed for Ambystoma tigrinum tigrinum (Eastern tiger salamander) and for A. mexicanum (Mexican axolotl) to generate the first comprehensive linkage map for these model amphibians. (genetics.org)
  • transcriptome
  • Consequently, the manner in which a transcriptome as a whole is expressed and regulated remained unknown until higher-throughput techniques were developed. (wikipedia.org)
  • libraries
  • This makes it difficult to write programs that can unambiguously determine that two EST libraries were sequenced from the same tissue. (wikipedia.org)
  • Genomics
  • D. L. Erdner and D. M. Anderson, "Global transcriptional profiling of the toxic dinoflagellate Alexandrium fundyense using massively parallel signature sequencing," BMC Genomics , vol. 7, article 88, 2006. (hindawi.com)
  • Moreover, molecular markers that are used to develop linkage maps provide the material for generating nucleotide probes and sequences that can be used in a variety of ways, including in situ hybridization, population genetics, systematics, molecular evolution, and functional genomics. (genetics.org)
  • Cells
  • ArgBP2 is highly abundant in cardiac muscle cells at sarcomeric Z-disc structures, and is expressed in other cells at actin stress fibers and the nucleus. (wikipedia.org)
  • In cells that already express high level of Oct3/4, exogenously transfected Oct3/4 will lead to the repression of Rex1. (wikipedia.org)
  • tissue
  • For instance, cancer origin of a library is often mixed with the tissue name (e.g., the tissue name "glioblastoma" indicates that the EST library was sequenced from brain tissue and the disease condition is cancer). (wikipedia.org)
  • Haptoglobin had been shown to be expressed in adipose tissue of cattle as well. (wikipedia.org)
  • databases
  • Here we describe a collection of 691 sequences from the Western corn rootworm midgut, 27% of which predict proteins with no matches in current databases. (unl.edu)
  • First opened to outside users in December 1989, the NCBI Experimental BLAST Network Service, running the latest BLAST software on SMP hardware against the latest releases of the major sequence databases, quickly established the NCBI as a convenient, one-stop shop for sequence similarity searching. (wikipedia.org)
  • Using MEGABLAST, Hi-Fi reads are then matched to sequences in annotated databases and any positive matches are then subtracted from the dataset. (wikipedia.org)
  • reads
  • The initial quality screen uses several general parameters to exclude ambiguous sequences, leaving behind a dataset of high-fidelity (Hi-Fi) reads. (wikipedia.org)
  • BLAST
  • At Washington University in St. Louis, Gish revolutionized similarity searching by developing the first BLAST suite of programs to combine rapid gapped sequence alignment with statistical evaluation methods appropriate for gapped alignment scores. (wikipedia.org)
  • generally, the remaining sequence is BLAST again with less stringent parameters to allow for slight mismatches (1 in 20 nucleotide). (wikipedia.org)
  • technology
  • The resulting sequence is a relatively low-quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides. (wikipedia.org)
  • single
  • This may be done to emphasize the value of the upgrade to the software user, or, as in Adobe's case, to represent a release halfway between major versions (although levels of sequence based versioning are not limited to a single digit, as in Drupal version 7.12). (wikipedia.org)
  • code
  • For instance, in a scheme that uses a four-sequence identifier, the first sequence may be incremented only when the code is completely rewritten, while a change to the user interface or the documentation may only warrant a change to the fourth sequence. (wikipedia.org)