Experiments vitro. Medical search. Frequent questions

Using pharmacological approaches along with in vitro and in vivo experiments, we found that Nogo-A/NgR1 signaling regulates the pace of neuronal neogeneration by reducing NSC proliferation. (jneurosci.org)
For this purpose, in vitro and in vivo experiments were performed. (cdc.gov)

In vitro methods, based on human primary cells, cell lines, and genetically modified reporter cell lines, have greatly expanded the scope of in vitro toxicology. (brill.com)
Unlike crude, archaic animal tests, non-animal methods usually take less time to complete, cost only a fraction of what the animal experiments that they replace cost, and are not plagued with species differences that make extrapolation difficult or impossible. (alv.org.au)

Tests performed on animals for medical research and teaching are so common in Australia that in 2017, over 20 million animals were experimented on. (alv.org.au)
4 An up-to-date list of WHO international biological reference preparations is available at http://www.who.int/bloodproducts/catalogue/en/ (accessed 11 May 2017). (who.int)

A mechanistic simulation engine for the in vitro dissolution experiment that predicts in vitro disintegration and dissolution of pharmaceutical dosage forms. (simulations-plus.com)
With the recent advances in human-cell cultivation techniques, allowing in vivo -like in vitro long-term functionality, there is a shift in focus towards the mechanistic details of the adverse effects "over time" aimed at a better understanding of the dynamics of biological processes. (brill.com)
Martin's research is focused on the nanoparticle-(mammalian)cell interaction, with a view to determining the mechanistic toxicological, immunological and genotoxic effects that nanoparticles, with varying physico-chemical characteristics, may cause at the cellular level by using advanced, next-level in vitro systems combined with state-of-the-art microscopy and bioanalytical approaches. (swansea.ac.uk)
Another clear ambition of Martin's research is to create, study and promote alternative in vitro models that mimic in vivo environments, in order to gain a mechanistic understanding of how nanoparticles may, potentially, adversely impact human health. (swansea.ac.uk)
Furthermore, by creating sophisticated in vitro multi-cellular models of different human organs in combination with state-of-the art microscopic and biochemical analyses, Martin is able to obtain a mechanistic understanding of the health risks posed by nanomaterials under realistic conditions, as well as gain valuable insight into the potential of in vitro as alternative test systems to in vivo methodologies. (swansea.ac.uk)

Therefore, the experimental conditions of in vitro studies used to assess hepatic uptake transport were optimized regarding active transport processes, nonspecific binding, and passive diffusion ( P dif ). (aspetjournals.org)
A detailed modeling & simulation program for in vitro metabolism & transport studies. (simulations-plus.com)
In vitro and in vivo studies like these help us understand better the device capabilities, although further clinical studies are required to verify received parameters. (ems-urology.com)
In vitro skin and eye irritation studies are available. (europa.eu)
These findings prompted us to perform in vitro studies to assess the effect of the electric current on the platinum portion of GDCs. (ajnr.org)
In-vitro metabolic inhibition studies using rat and human liver microsomes and intestinal absorption studies of riluzole in a rat everted gut-sac model were conducted to elucidate the mechanism of interaction. (researchgate.net)
In-vitro metabolism studies demonstrated significant inhibition of riluzole metabolism when it was co-incubated with ciprofloxacin or grapefruit juice. (researchgate.net)
Carefully designed clinical studies are warranted to establish the clinical significance of these in vitro findings. (aspetjournals.org)
In vitro studies demonstrate that exogenous recombinant Gal3 is internalised by neuronal cell lines and primary neurons where it interacts with endogenous αSyn fibrils. (lu.se)
In line with our in vitro studies, under these conditions, genetic deletion of GAL3 leads to increased intracellular αSyn accumulation within dopaminergic neurons and remarkably preserved dopaminergic integrity and motor function. (lu.se)
Mn accumulates in the inner ear (cochlea) following acute in vivo treatment and in vitro studies show that uM levels of Mn damage sensory hair cells and spiral ganglion neurons (SGN) in the inner ear. (cdc.gov)
FEM has been shown to be a useful tool when investigating complex systems that are difficult to standardize during in vitro and in vivo studies. (bvsalud.org)
These are followed up by in vitro experiments and studies on human CSF and plasma. (lu.se)
Our work also spans from human brain tissue and cohort studies to in vitro and animal studies which is line with Multiparks translational initiative. (lu.se)

Our internally consistent findings between working-heart and trabecula experiments explain the rapid improvement of LV systolic function observed in patients with chronic pulmonary hypertension following surgical relief of RV pressure overload. (frontiersin.org)
This in vitro study was inspired by the histologic and SEM findings in a patient with subarachnoid hemorrhage who died 16 h after GDC treatment of a basilar tip aneurysm. (ajnr.org)
Our findings were subsequently tested by in vitro experiments, where putative early and later-stage DN1 progenitors from the thymus were purified and their progression into DN2 was measured. (lu.se)

Special focus is also given to creating disease-based in vitro models and the role nanoparticles play in disease onsert and/or exacerbation. (swansea.ac.uk)

In this study, we investigated the in vitro inhibitory effects of the individual major cannabinoids and metabolites ∆9-tetrahydrocannabinol (THC), cannabidiol (CBD), cannabinol (CBN), 11-nor-THC-carboxylic acid, and 11-hydroxy-THC on CES1 activity. (aspetjournals.org)

In this study, we integrated plasma concentrations, in vitro/in vivo data for receptor or protein binding, and in silico data, using a physiologically based pharmacokinetic model, to examine the predictability of receptor occupancy in humans. (simulations-plus.com)
We exposed synchronized ring-stage parasites for 72 h across a range of dihydroartemisinin concentrations (0-1 µmol/L) in duplicate or triplicate and in ≥3 independent experiments. (cdc.gov)

One of the validated in vitro skin irritation tests is the EPISKIN test, which is recommended in international guidelines (e.g. (europa.eu)

Association and dissociation rate constants and unbound fractions in the serum and brain were determined in vitro/in vivo using human D2 receptor-expressing membrane fractions, human serum and mouse brain. (simulations-plus.com)
The first pregnancy achieved through in vitro human fertilisation of a human oocyte was reported in The Lancet from the Monash University team of Carl Wood, John Leeton and Alan Trounson in 1973, although it lasted only a few days and would today be called a biochemical pregnancy. (wikipedia.org)
SV40 Transfected Human Anterior Cruciate Ligament Derived Ligamentocytes-Suitable as a Human in Vitro Model for Ligament Reconstruction? (mdpi.com)
With further technological developments, these human in vitro cellular models shall be highly useful in the screening of compounds for personalized medicine, allowing optimum therapy with minimum or no adverse effects, and in the study of adverse outcomes in different strata of population. (brill.com)
An in vitro electron microscopic study of human blood was performed to elucidate the role that electrothrombosis might play in triggering the biologic response of thrombosis of the aneurysmal sac. (ajnr.org)
Using human blood, an in vitro scanning electron microscopic (SEM) study was performed to elucidate the role of electrothrombosis in the treatment of aneurysms. (ajnr.org)
Therefore, in this study, we assessed the ability of human adipose mesenchymal stem cells (hADSCs) to express the characteristics of ECs after induction in vitro to evaluate the theoretical feasibility of maintaining or restoring the function of ECs after intraocular application. (molvis.org)
The Expert Committee on Biological Standardization reviews developments in the field of biological substances used in human medicine, which include vaccines, biological therapeutics, blood products and related in vitro diagnostic devices. (who.int)

We aimed to stop superficial bleeding from minor abra- elucidate if the in vitro properties of alum sions or cuts. (who.int)

To explicit cell apoptosis trend in PC12 oxygen-glucose-serum deprivation/restoration (OGSD/R) model and provide experimental bases for neural cell simulation in ischemia reperfusion injury in vitro . (oncotarget.com)

The use of in vitro data for quantitative predictions of transporter-mediated elimination in vivo requires an accurate estimation of the transporter Michaelis-Menten parameters, V max and K m , as a first step. (aspetjournals.org)
The model was used to estimate kinetic parameters of in vitro transport data from organic anion-transporting peptide model substrates (e.g., cholecystokinin octapeptide deltorphin II, fexofenadine, and pitavastatin). (aspetjournals.org)
In the present study, we approached the preparation of leukosomes using a design of experiment (DoE) method to better understand the influence of experimental parameters on leukosomes features such as size, size distribution, and protein loading. (frontiersin.org)

They were tested during treatment of artificial renal stones in the experimental in vitro and in vivo porcine models. (ems-urology.com)
Experimental work started with short term single driver experiments but soon developed to include multiple drivers and sometimes even successive generations in an attempt to address as much of climate change's complexity as possible and include adaptive, trans-generational aspects to project physiological reaction norms into the future. (easychair.org)
For the experimental design and integrating the data from a range of experiments, they developed a special modelling methodology for protein-protein docking to simulate the formation of chaperone complexes. (uni-heidelberg.de)
These experiments showed that the two DN1 sub-populations divided with similar rates, but progressed to the DN2 stage with different rates, thus providing experimental evidence that DN1 cells increase their commitment probability in a cell-intrinsic manner as they undergo cell division. (lu.se)

Nevertheless, these limitations in the control incubations performed at low temperature have not been addressed systematically in uptake experiments up to now. (aspetjournals.org)
A panel of DAbR1-expressing T cells and CAR T cells exhibited greater than 8-fold increased uptake of 86 Y-AABD in vitro when compared with nontransduced cells. (snmjournals.org)

failed verification] In 1958, Anne McLaren and John Biggers published a landmark paper in the journal Nature, outlining how they had successfully grown mouse embryos in vitro and transferred them into female mice. (wikipedia.org)
Here, also proof-of-concept experiments have been successfully conducted in vitro. (uni-ulm.de)

In this study, dendritic adaptation was experimentally examined at a higher stimulation frequency, 5 Hz, using the training pattern of previous experiments run at 0.5 to 1 Hz. (nature.com)
This study aimed to use network pharmacology to predict the therapeutic mechanism of oroxyli semen (OS) on triple-negative breast cancer (TNBC) and validate it through in vitro experiments. (bvsalud.org)
So far, no in vivo experiment has been done to study the effect of shear force on the spine. (asme.org)
The goal of this study is to design an in vitro experiment that will provide incessant torques in the calves' tails, and determine the relationship between the magnitude of the torque and change of stress between tail segments. (asme.org)
Hence, the objectives of this study were to determine the in vivo and in vitro effects of live pigeon pox (PP) virus as well as the in vitro effect of live Newcastle disease (ND) virus on parthenogenesis. (scialert.net)

Molecular mechanism of oroxyli semen against triple-negative breast cancer verified by bioinformatics and in vitro experiments. (bvsalud.org)
Here, we investigated the antiproliferative activity and mechanism of Rh4 against CRC in vivo and in vitro. (hindawi.com)

Online dose determination for in vitro experiments with nano p. (kit.edu)

Prediction of CNS occupancy of dopamine D2 receptor based on systemic exposure and in vitro experiments. (simulations-plus.com)

Their in-vitro experiments uncovered a multi-stage biochemical process in which protein molecules are dissolved from the aggregates. (dkfz.de)
The researchers succeeded in identifying a previously unknown, multi-component protein complex that efficiently solubilizes stress-induced protein aggregates in vitro. (dkfz.de)

Pharmacological experiments show that Nogo-66/NgR1 interaction reduces the proliferation of NSCs. (jneurosci.org)
100 publications within the field of nanotoxicology and the nanoparticle-cell interaction in vitro . (swansea.ac.uk)

In Experiment 1, we treated rats with 10 g/L MnCl2 in drinking water for 60 or 90 days and used inductively coupled plasma mass spectrometry to quantify the increase in Mn levels in three regions of the cochlea and three brain regions. (cdc.gov)

Stones were located inside the tube and the experiment was performed with each device one by one. (ems-urology.com)

In Experiment 2, we employed a 50% higher dose of MnCl2 (15 g/L) in drinking water and found similar results to Experiment 2. (cdc.gov)

In-Vitro, experiments were also carried out to corroborate the clinical results. (medicalnewstoday.com)

Researchers used them both in vitro and in-vivo during the PCNL of artificial renal stones (BegoStone Plus, Bremen, Germany). (ems-urology.com)

Indeed, in vitro experiments proved that MIF significantly increases the attraction of eosinophils. (sciencedaily.com)

OGSD/R model was constructed using the passage PC12 cells in vitro . (oncotarget.com)
In vivo experiment was performed using a porcine model. (ems-urology.com)

We also validated this formulation technologically and tested its behavior in in vitro colorectal cancer (CRC) models, including CRC patient-derived tumor organoids (PDOs). (frontiersin.org)
We employed in vitro cultures and two in vivo models to investigate whether androgens exert these effects directly on marrow lymphoid precursors or whether actions on marrow stromal elements are required. (jci.org)

A series of column experiments were conducted to investigate the depth distribution of different stygofauna (Syncarida and Copepoda) under saturated conditions and after fast and slow water table declines. (edu.au)
In contrast, under both ex vivo and in vitro conditions, where the effect of RV pressure overload was circumvented, we found that LV was capable of developing normal systolic pressure and stress. (frontiersin.org)
Moreover, under in vitro conditions, live ND virus was found to exert similar effects as live PP virus. (scialert.net)

The in vivo effect of live PP virus following vaccination and the in vitro effects of live PP and ND viruses following direct administration over the germinal disc of cultured quail eggs on parthenogenesis were determined. (scialert.net)

Although there have been a significant number of in vitro and in vivo assessments of the effects of cannabis on cytochrome P450 and UDP-glucuronosyltransferase enzyme systems, there is limited information regarding the effects of cannabis on the major hepatic esterase, carboxylesterase 1 (CES1). (aspetjournals.org)
Further experiments revealed that bone marrow stromal cells produced TGF-β in response to dihydrotestosterone (DHT), and neutralization of TGF-β in the DHT-treated stromal cells reversed the suppressive effects. (jci.org)
The stromal cell requirement for androgen-mediated effects was confirmed in vivo by experiments using chimeric animals created by bone marrow transplantation in which androgen receptor expression was restricted to either the stromal or lymphoid cells of the bone marrow. (jci.org)
These experiments suggest that effects of androgens on developing B cells are mediated through androgen receptors in bone marrow stromal cells. (jci.org)

The expenses involved in breeding, feeding, housing, experimenting on and then disposing of the bodies all add up to excessive amounts, especially if the test takes months or even years. (alv.org.au)

The tests are often conducted without pain relief and animals are usually killed once the experiment is completed. (alv.org.au)

In addition, aggregation experiments show that Gal3 affects spatial propagation and the stability of pre-formed αSyn fibrils resulting in short, amorphous toxic strains. (lu.se)

The design of experiment (DoE) strategy can overcome these limitations. (frontiersin.org)
Many pieces created by Verner Panton, who died in 1998, are housed in the Schaudepot - the visible storage facility of the Vitra Design Museum, which also holds a large part of his estate. (vitra.com)

As in the in vitro setting, a significant difference for SCT was found between soft and hard stones. (ems-urology.com)

Experiments on animals for medical purposes are not only cruel they are unreliable. (alv.org.au)
Experiments on Animals - Why Are They Still Done? (alv.org.au)

Advanced in vitro experiments confirmed the products' compatibility with skin. (europa.eu)

L'agrégation plaquettaire induite par le collagène dans des échantillons de plasma riche en plaquettes de 14 lapins sains a été mesurée par turbidimétrie en utilisant un agrégomètre, avant et une heure après une injection intra- veineuse d'alun. (who.int)

During the past 35 years he designed numerous pieces of furniture, series of dishes, lights, watches and other objects of everyday-use for companies as Vitra, Alessi or Cappellini. (idz.de)

The oncolytic adenovirus ICOVIR-15K was engineered to express an EGFR-targeting BiTE (cBiTE) antibody under the control of the major late promoter, leading to generation of ICOVIR-15K-cBiTE, which retained its oncolytic properties in vitro . (aacrjournals.org)

Expert biocurators play a crucial role in IDP databases by providing a direct interpretation of disorder derived from structural experiments and manually curating these ID annotations. (iucr.org)

In vitro prevascularization can result in faster integration with the host vascular network [ 14 ]. (hindawi.com)

Vivo experiments2

METHODS2

20172

Mechanistic5

Studies14

Findings3

Nanoparticles1

Inhibitory1

Concentrations2

Irritation1

Human8

Elucidate1

Simulation1

Parameters3

Experimental4

Uptake2

Successfully2

Study5

Mechanism2

Determination1

Exposure1

Protein2

Interaction2

Brain1

Device1

Dose1

Clinical1

Researchers1

Significantly1

Model2

Models2

Conditions3

Effect1

Effects4

Bodies1

Tests1

Show1

Design2

Found1

Animals2

Skin1

Plasma1

Companies1

Properties1

Role1

Faster1