• We use in vitro and in vivo models, including live cell metabolic assays and transgenic models. (academiccareers.com)
  • We are currently testing this hypothesis using genetic, biochemical, and transgenic assays. (nyu.edu)
  • We are using in vivo mouse transgenic assays to examine particular HACNSs from our current dataset for human-specific gene regulatory functions by comparison to chimpanzee and rhesus orthologs (Fig. 3). (yale.edu)
  • Male Development of Chromosomally Female Mice Transgenic for Sry gene' (1991), by Peter Koopman, et al. (asu.edu)
  • Candidate clusters are cloned into reporter genes, transformed into the genome, and tested for in vivo activity by in situ hybridization experiments. (nyu.edu)
  • 2003). The therapeutic effects of different genes delivered by ment of the hGFAP promoter used here has been extensively studied and lentiviral vectors have been documented in a number of animal found to be up-regulated after several different stimuli both in vitro and models, both in rodents and primates, and examples include delivery in vivo in transgenic mice (Brenner et al. (lu.se)
  • To model the effect in vivo, the authors used a transgenic mouse which over-expressed UMOD leading to salt-sensitive hypertension and interstitial nephritis . (medscape.com)
  • Many genes with functions in abiotic stress tolerance were identified, and overexpression of these genes confers increased drought tolerance in transgenic plants. (usda.gov)
  • The most common fields in transgenic livestock that are being researched are: growth, pathogen resistance and quality of meat products. (freeonlineresearchpapers.com)
  • The team of scientists used the methods identified during the Dolly experiments to produce transgenic livestock capable of producing milk containing human blood clotting factor IX, which helps to treat a type of hemophilia. (asu.edu)
  • Their capacity to elicit immune and protective responses was studied using immunization of HLA-A*11:01 transgenic mice. (jci.org)
  • Yamanaka induced somatic cells to act like human embryonic stem cells (hESCs), allowing researchers to experiment with non-embryonic stem cells with a similar capacity as hESCs. (asu.edu)
  • In the 1990s, researchers working at the Roslin Institute in Edinburgh, Scotland, performed cloning experiments in collaboration with PPL Therapeutics in Roslin, Scotland, on human coagulation factor IX, a protein. (asu.edu)
  • The researchers investigated the survival of DNA from transgenic maize and the transfer of the antibiotic resistance bla gene to bacteria in the presence of saliva, rumen fluid and silage effluent, which are relevant to horizontal gene transfer in the oral cavity, the rumen, and in silage. (gmwatch.org)
  • The Lund University Transgenic Core Facility (LUTCF) is a core resource whose primary goal is to deliver most up-to-date methodologies in transgenic technology to both our researchers and external users. (lu.se)
  • Knockout and other transgenic mice are traditionally made by genetically modifying embryonic stem cells (ESCs) in a dish and then inserting them into a recipient mouse embryo. (the-scientist.com)
  • It rather serves as a basis for requirements that need to be met in order to use animals in experiments. (aerzte-gegen-tierversuche.de)
  • Given the significant differences in organ function and metabolism between animals and humans, the results of such experiments do not accurately predict the toxicity for humans. (aerzte-gegen-tierversuche.de)
  • Genetically modified animals now constitute approximately 45% of all animals used in experiments. (aerzte-gegen-tierversuche.de)
  • The experiment was meant to prove the principle of transgenic animals, particularly dogs, who, due to their lifespan and reproductive cycle, are good stand-ins for human disease research. (mentalfloss.com)
  • There also was a huge public opinion formed on transgenic animals. (freeonlineresearchpapers.com)
  • Public view of transgenic animals is that they maybe harmful to both humans and the environment. (freeonlineresearchpapers.com)
  • Americans have never eaten transgenic animals before, so policies on how to safely approve them as dinners just don't exist. (good.is)
  • Passenger mutations affect experiments using transgenic animals models called congenic mice, whose modified genetic material comes from a different genetic background than the rest of their genome. (the-scientist.com)
  • Transgenic animals have identified a role for specific manufacturing, auto body painting and repair and immunity, including the involvement of T-helper type 1/2 plastics manufacturing. (cdc.gov)
  • HBsAg was the first viral antigen chosen to be produced in transgenic plants, firstly in tobacco [3] and subsequently in lupin callus and lettuce adapted to colder climates [4]. (scielo.br)
  • There are a number of hypothesized that regulating the transgene with a GFAP promoter in a different vectors that transduce cells in the brain in a slightly different viral vector would give rise to a high transgenic expression in the manner (for a review see, e.g. (lu.se)
  • We conducted studys in Texas and Arizona, two significantly different production environments and found that the timing of drought stress is critical for IPT-transgenic cotton to display its yield advantage over non-transgenic control plants. (usda.gov)
  • When water deficit stress occured before flowering (vegetative phase), the IPT-transgenic cotton plants had higher yields compared to non-engineered plants. (usda.gov)
  • It was found that the timing of water deficit stress is critical for IPT-transgenic cotton to display its yield advantage over control plants (i.e. wild-type and segregated non-transgenic plants). (usda.gov)
  • however, if water deficit stress occurs at or after flowering (reproductive phase), there would not be a yield difference between IPT-transgenic and control cotton plants. (usda.gov)
  • The nptII gene in transgenic potato plants coding for kanamycin resistance, transforms naturally competent cells of the soil bacteria Pseudomonas stutzeri and Acinetobacter BD413 (both harboring a plasmid with an nptII gene containing a small deletion (hence nonfunctional) with the same high efficiency as nptII genes on plasmid DNA ( 3x10-5 -1x10-4) despite the presence of a more than 106 fold excess of plant DNA. (gmwatch.org)
  • The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. (scielo.br)
  • The scientists designed experiments using genetic approaches and transgenic flies to remove the enzyme MOF from fly mothers. (mpg.de)
  • The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)
  • Previous experiments had revealed that the male pheromone cis-vaccenyl acetate activates the glomerulus DA1 in the brains of female flies. (scitechdaily.com)
  • The incumbent will conduct research and ensure that all experiments are appropriately conducted following the policies and procedures of Stony Brook University. (academiccareers.com)
  • We describe specific factors of experimental releases of mosquitoes that we believe are critical to inform institutional biosafety committees and similar review boards to which proposals to conduct mosquito release experiments have been submitted. (cdc.gov)
  • Thousands of specific transgenic mouse lines exist. (aerzte-gegen-tierversuche.de)
  • Indeed, converging data from genomics, neuropathology, cell biology and transgenic mouse experiments suggest that the microglial response is a fundamental driver of disease progression. (imperial.ac.uk)
  • Selected HACNSs are tested for enhancer activity using standard mouse transgenic strategies. (yale.edu)
  • A detailed comparison of lhx5 -driven and vGLut2a -driven GFP in transgenic reporter lines revealed ancestral topological relationships between the thalamic eminence (EmT), the medial amygdala (MeA), the nLOT, and the integrative olfactory pallium. (frontiersin.org)
  • Such experiments will examine how microgravity affects the rodents, providing information relevant to human spaceflight, discoveries in basic biology, and knowledge that can help treat human disease on Earth. (wikipedia.org)
  • by transgenic plant DNA strictly depends on homologous sequences in the recipient cells. (gmwatch.org)
  • The high failure rate in commercializing the genes that were promising in laboratory experiments is likely due to negative results from the field trials due to the complex nature of a multitude of stressors in production settings and disparate climate events over multiple years of field trials. (usda.gov)
  • The marker genes were removed upon activation of a recombinase from the transgenic intermediate product. (admin.ch)
  • In our first experiments, we have focused on the Bicoid gradient (Figure 2) and on gradients of transcription factors encoded by the gap genes hunchback, Kruppel, knirps, and giant. (nyu.edu)
  • In our first experiments, we have used genetic experiments to flatten the Bcd gradient, and then measured the amounts of Bcd required for activating specific target genes. (nyu.edu)
  • DcAOX genes' were further explored in a plant pot experiment in response to chilling, which confirmed the early AOX transcript increase prior to the induction of a specific anti-freezing gene. (frontiersin.org)
  • One passage on transgenic mice expressing bovine prion protein ( 7 ). (cdc.gov)
  • To visualize the glutamatergic neurons, we used MS-DBB slices from transgenic mice in which the green fluorescent protein is expressed specifically by vesicular glutamate transporter 2-positive neurons and characterized their properties using whole-cell patch-clamp technique. (jneurosci.org)
  • To address these questions, we used transgenic mice in which the expression of the VGLUT2 gene is linked with that of the enhanced green fluorescent protein (eGFP). (jneurosci.org)
  • We created and tested multi-epitope DNA or protein vaccines with TLR4 ligand emulsion adjuvant (gluco glucopyranosyl lipid adjuvant in a stable emulsion [GLA-SE]) for their ability to protect against Toxoplasma gondii in HLA transgenic mice. (jci.org)
  • A ) HLA-A*11:01 transgenic mice immunized with LO protein plus GLA-SE were protected compared with control mice inoculated with PBS when they were challenged with 20,000 T . gondii prugneaud strain (Fluc) luciferase expressing parasites after 4 and 6 days. (jci.org)
  • The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
  • Transgenic animal models expressing rhodopsin glycosylation mutants also exhibit light exacerbated retinal degeneration (RD). In this study, we used transgenic Xenopus laevis to investigate the pathogenic mechanism connecting light exposure and RD in photoreceptors expressing T4K or T17M rhodopsin. (jneurosci.org)
  • The UMOD story got a lot more interesting when Trudu et al published an intriguing set of experiments establishing a link between uromodulin, hypertension, and kidney disease via activation of the renal sodium co-transporter NKCC2. (medscape.com)
  • Transgenic fly lines were crucial for the experiments. (scitechdaily.com)
  • Transgenic Chinese hamster ovary cell lines overexpressing eIF4E were resistant to Cd-induced cytotoxicity and cell death. (cdc.gov)
  • What are animal experiments? (aerzte-gegen-tierversuche.de)
  • The experiments do not serve the well-being of the animal, but aim to answer a wide variety of the researcher's scientific questions. (aerzte-gegen-tierversuche.de)
  • For what purpose are animal experiments conducted? (aerzte-gegen-tierversuche.de)
  • Basic research has accounted for more than half of all animal experiments in Germany for years. (aerzte-gegen-tierversuche.de)
  • This is due to the fact that, as proven by many scientific studies, the transferability of findings from animal experiments to humans is by no means assured. (aerzte-gegen-tierversuche.de)
  • Also, this kind of animal experiments is unnecessary. (aerzte-gegen-tierversuche.de)
  • AquaBounty never released information about the outbreak to the public, despite the fact that its GE salmon is poised to become the first transgenic animal to be approved for human consumption. (good.is)
  • Rats transgenic for Huntington's disease (tgHD51 CAG rats), surviving up to two years, represent an animal model of HD similar to the late-onset form of human disease. (hindawi.com)
  • Twenty mice were flown for this experiment and the study lasted 33 days. (wikipedia.org)
  • The new tool "empowers the scientific community to look a little closer" at the transgenic mice they are using, said Soren Warming , a senior scientist at Genentech who was not involved in the study. (the-scientist.com)
  • In this study we used a lentiviral vector that drives transgenic populations (BloÈmer et al. (lu.se)
  • These experiments have also provided evidence of functional differences between human and chimpanzee orthologs. (yale.edu)
  • Unlike some lab models, the mice used in Mayo Clinic's multiple myeloma model have functioning immune systems, making it a transgenic model. (mayo.edu)
  • The Cell Phenotyping arm of this module is designed to provide technical expertise and assistance with ongoing cell phenotyping experiments. (utsouthwestern.edu)
  • The cell sorter has 4 lasers providing flexibility for experiments with up to 6 colors and with multiple sort collection formats. (utsouthwestern.edu)
  • Survival of free DNA encoding antibiotic resistance from transgenic maize and the transformation activity of DNA in ovine saliva, ovine rumen fluid and silage effluent. (gmwatch.org)
  • Materials and methods approach to regulate transgenic expression. (lu.se)
  • The experiment consists of a 5-minute reaction time task that allows crewmembers to monitor the daily effects of fatigue on performance while on ISS. (nasa.gov)
  • In contrast to wild-type mice, the transgenic UMOD mice had marked improvement in blood pressure with furosemide (an inhibitor of NKCC2). (medscape.com)
  • Béringue V , Le Dur A , Tixador P , Reine F , Lepourry L , Perret-Liaudet A , Prominent and persistent extraneural infection in human PrP transgenic mice infected with variant CJD. (cdc.gov)
  • Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts' (1997), by Angelika E. Schnieke, et al. (asu.edu)
  • Many more applications of transgenic technology are now being considered as the field expands almost exponentially. (freeonlineresearchpapers.com)
  • Interestingly, when IPT-transgenic cotton was field-tested at two different sites, Texas and Arizona, different results were obtained. (usda.gov)
  • the release of transgenic mosquitoes is another experiment with unpredictable results. (esquerda.net)
  • Results of Western blot analysis and immunoprecipitation experiments demonstrated a significant induction of ubiquitination of eIF4E in the Cd treated cells. (cdc.gov)
  • The breakthrough that led to development of the transgenic model involves a gene known as MYC. (mayo.edu)
  • It also does not address releases of transgenic or exotic (non-native) mosquito species, both of which require particular regulatory approval. (cdc.gov)
  • These experiments are focused on three simple questions: First, do gradients really function as morphogens? (nyu.edu)
  • The successful applicant will assist the Principal Investigator in the experiments, data analysis, and manuscript preparation. (academiccareers.com)
  • In contrast, T4K and T17M rhodopsins express at high levels, exhibit little or no misfolding and localize primarily to the OS in transgenic frog rods suggesting that ER stress may not play a major role in their pathology. (jneurosci.org)
  • To explain this phenomenon, reduced irrigation experiments with different timing in applying water deficit stress were conducted. (usda.gov)
  • Numerical data for all behavioral and calcium imaging experiments shown in Figs 1 - 4 and S1 - S5 are provided in https://doi.org/10.5281/zenodo.6537728 . (plos.org)
  • All three contributors to vectorial capacity can be estimated vide guidance for evaluating their safety and practices to in a single experiment method using MRR. (cdc.gov)
  • CDR Skvortsov began the day by attending to the current experiment session with the KPT-21 Plasma Crystal-3+ (Plazmennyi-Kristall/PK-3+) payload, activating the turbopump in the MRM2 "Poisk" module for keeping the vacuum chamber (EB) evacuated. (nasa.gov)