Experiments human genome interspersed repetitive sequences. Medical search. Frequent questions

In many organisms, a significant fraction of the genomic DNA is repetitive, with over two-thirds of the sequence consisting of repetitive elements in humans. (wikipedia.org)
We performed deep genomic DNA sequencing of CD-1 mice and used the data to identify genome-wide SNPs, indels, and germline transposable elements relative to the mm10 reference genome. (biomedcentral.com)
Mapping of transcriptomic and genomic data derived from CD-1 mice to the standard mouse reference genome is therefore subject to inaccuracies and potentially confounding effects. (biomedcentral.com)
We performed deep genomic DNA (gDNA) sequencing in order to map both the uniform and non-uniform variants of CD-1, including SNPs, insertions and deletions (indels), and germline transposable elements (TEs). (biomedcentral.com)
Problems that arise during DNA replication can drive genomic alterations that are instrumental in the development of cancers and many human genetic disorders. (elifesciences.org)
Taxonomic classification of those E. ictaluri specific phages must rely on a synthesis of morphological and genomic details, taking into consideration that phage evolution continues to be profoundly directed by lateral gene transfer, and that a rational hierarchical technique of phage classification really should be based within the degree of DNA and protein sequence identity for several genetic loci. (hivprotease-signal.com)
Celecoxib structure A comparative genomic evaluation involving the genome of phage eiAU and that of phage SSL 2009a unveiled that genome regions encoding many putative structural and replica tion proteins are shared by both phages. (hivprotease-signal.com)
The general genomic organization of the three new phages is shared by several members in the Siphoviridae loved ones of phages sequenced to date, and is supported through the previously described morphol ogy of these phages. (hivprotease-signal.com)
Here, we present a complete Nautilus pompilius genome as a fundamental genomic reference on cephalopod innovations, such as the pinhole eye and biomineralization. (nature.com)
The nautilus genome constitutes a valuable resource for reconstructing the evolutionary scenarios and genomic innovations that shape the extant cephalopods. (nature.com)
Therefore, genome studies of N. pompilius would not only shed light on the origin and evolution of cephalopod genomic novelties but also incentivize research on their biology and inform sustainable conservation. (nature.com)
Since then, advances in technology have made genomic sequencing cheaper and faster. (cilecenter.org)
Different variants of FISH can be used to retrieve information on genomes from (almost) base pair to whole genomic level, as besides only second and third generation sequencing approaches can do. (frontiersin.org)
These data provide a foundational map of large SV in the morbid human genome and demonstrate a previously underappreciated abundance and diversity of cxSV that should be considered in genomic studies of human disease. (biomedcentral.com)
Recent research has revisited the topic of TE impact on gene expression, noting that the dissemination of highly similar sequence accomplished by TEs is a powerful way to link many diverse genomic regions into a regulatory network [ 7 ]. (biomedcentral.com)
In a number of cases, extant TE sequences have integrated with established genomic functions and been co-opted by the genome for critical roles [ 7 ],[ 8 ]. (biomedcentral.com)
All targeted genome engineering relies on the introduction of a site-specific double-strand break (DSB) in a pre-determined genomic locus by a rare-cutting DNA endonuclease . (ac.ir)
Targeted genome engineering or editing enables researchers to modify genomic loci of interest in a precise manner, which has a turning point in medicine, biological research, and biotechnology. (ac.ir)
During the past decade, two major developments-the emergence of massively parallel, ultra-cheap DNA sequencing technologies and the use of these technologies as digital readouts for functional genomic assays-have led to a profusion of data describing various features of genomes, epigenomes, and transcriptomes 1 , 2 . (biorxiv.org)
Polymorphic sites tend to be sparse along the genome, however, so this approach requires some type of pooling of information across genomic positions, which can be difficult in the absence good-quality genomic annotations. (biorxiv.org)

We then used the identified variants to construct a strain-specific CD-1 reference genome, and associated annotation files, including genes and the full set of identified CD-1 variants. (biomedcentral.com)
Nautilus shows a compact, minimalist genome with few encoding genes and slow evolutionary rates in both non-coding and coding regions among known cephalopods. (nature.com)
While our genes are encoded by a varying sequence of nucleotides, the genotype is determined by the DNA sequence, both nuclear and mitochondrial. (cilecenter.org)
10,200 protein-encoding genes were predicted based on the genome sequence. (springeropen.com)
The genes and proteins were predicted and annotated based on the genome sequence. (springeropen.com)
Post-transcriptional regulation of human genes by TE-derived sequences has been observed in specific contexts, but has yet to be systematically and comprehensively investigated. (biomedcentral.com)
Cytogenetic (FISH) evidence and the genome assembly itself place core MHC genes on as many as four chromosomes with TAP and Class I genes mapping to different chromosomes. (biomedcentral.com)
Only about 10,000 of these genes have been annotated by the sea urchin sequencing team. (blogspot.com)
Sequences of DNA in the genes that are located between the EXONS. (lookformedical.com)
To date, most gene therapy trials have utilized viral vectors for permanent gene transfer due to their high transduction rate and their ability to integrate therapeutic genes into host genomes for stable expression. (biomedcentral.com)
The first stage involved identifying, in unrelated subjects showing linkage to 2q24-q33, genetic variants in exons and flanking sequence within candidate genes and comparing the frequency of the variants between autistic and unrelated nonautistic subjects. (neurotransmitter.net)
is it a wonder that our genome too is filled with the remains of extinct genes? (drwile.com)

In contrast, whole genome sequencing offers sensitive views of the genetic differences between strains ofM. (9lib.org)
tuberculosis strains in whole genome sequencing data. (9lib.org)
Using long-insert jumping libraries at 105X mean physical coverage and linked-read whole-genome sequencing from 10X Genomics, we document seven major SV classes at ~5 kb SV resolution. (biomedcentral.com)

In 1972 the late geneticist Susumu Ohno coined the term "junk DNA" to describe all noncoding sections of a genome, most of which consist of repeated segments scattered randomly throughout the genome. (blogspot.com)

In the 2000s, the data from full eukaryotic genome sequencing enabled the identification of different promoters, enhancers, and regulatory RNAs which are all coded by repetitive regions. (wikipedia.org)
Completion of eukaryotic genome sequencing projects is a difficult task, due to the large size of the genomes and the abundance of repeats in the genomes. (biomedcentral.com)

MIRU-VNTR analysis, on the other hand, uses mini-satellite sequences (tandem repeats of 10-100 nucleotides) dispersed at multiple loci in the bacterial genome. (frontiersin.org)
Transcript profiling revealed that the majority of up-regulated loci in mom1 carry sequences related to transposons and homologous to the 24-nt siRNAs accumulated in wild-type plants that are the hallmarks of RNA-directed DNA methylation (RdDM). (omicsdi.org)
Here, by using a genome tiling array, we showed that a subset of heterochromatin loci are silenced by the action of Morpheus' molecule 1 (MOM1) that is an epigeneic regulator for transcriptional gene silencing independent of global DNA and histone modification. (omicsdi.org)
However, mammalian piRNA sequences and piRNA-producing loci evolve more rapidly than the rest of the genome for unknown reasons. (gokcumenlab.org)
Our results indicate that genetic instability at pachytene piRNA loci, while producing certain pathogenic SVs, also protects genome integrity against TE mobilization by driving the formation of rapid-evolving piRNA sequences. (gokcumenlab.org)
While most pachytene piRNAs lack obvious targets, neither the copy numbers nor nucleotide sequences of pachytene piRNA loci are conserved 5 , 6 , 7 , and many of them are not found in syntenic regions even in closely related mammals 8 , 9 . (gokcumenlab.org)
Such proposed functions are difficult to reconcile with pachytene piRNAs' rapid evolution, and this rapid evolution and their redundant distribution across multiple loci on the genome also complicates their functional study. (gokcumenlab.org)

Like mammalian genomes, the sea urchin genome contains a lot of junk DNA, especially repetitive DNA. (blogspot.com)
The non-overlapping targeting preference of piggyBac and Tol2 makes them complementary research tools for manipulating mammalian genomes. (biomedcentral.com)

Further studies aimed at exploring genetic diversity and natural history of gemycircularviruses in human hosts are needed. (cdc.gov)
Outbred stocks provide a good model for outbred human populations, but inbred mouse strains are typically preferred in toxicology, pharmacology, and fundamental biomedical research due to lower genetic and phenotypic variability. (biomedcentral.com)
However, the prior study only examined single nucleotide polymorphisms (SNPs) previously identified in other mouse strains, and to date, a full, genome-wide identification of genetic variants that exist between CD-1 mice has not been done. (biomedcentral.com)
In addition, the standard reference genome in mouse was derived from the inbred C57BL/6 J strain, and the full set of genetic variants between CD-1 and C57BL/6 J are not known. (biomedcentral.com)
The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. (lookformedical.com)
Sequence homology is an indication of the genetic relatedness of different organisms and gene function. (lookformedical.com)
DNA transposons are natural genetic elements residing in the genome as repetitive sequences. (biomedcentral.com)
A commonly used genetic typing test that uses antibodies to detect variations on the surface of human red blood cells. (ojp.gov)

This generates a vast repertoire of transcripts that includes protein-coding messenger RNAs, long non-coding RNAs (lncRNAs) and repetitive sequences, such as SINEs (short interspersed nuclear elements). (nature.com)
Some regions of the genome encode non-protein-coding RNA like transfer RNAs, the RNA components of ribosomes, microRNAs and small interfering RNAs that regulate translation. (cilecenter.org)
RNA-directed DNA methylation (RdDM) is a process that uses 24-nucleotide (nt) small interfering RNAs (siRNAs) to induce de novo methylation to its homologous DNA sequences. (omicsdi.org)

Tandem repeats may vary in the number of nucleotides comprising the repeated sequence, as well as the number of times the sequence repeats. (wikipedia.org)
When the repeating sequence is only 2-10 nucleotides long, the repeat is referred to as a short tandem repeat (STR) or microsatellite. (wikipedia.org)
When the repeating sequence is 10-60 nucleotides long, the repeat is referred to as a minisatellite. (wikipedia.org)
In addition, fitCons scores indicate that 4.2-7.5% of nucleotides in the human genome have influenced fitness since the human-chimpanzee divergence, and, in contrast to several recent studies, they suggest that recent evolutionary turnover has had limited impact on the functional content of the genome. (biorxiv.org)
Many computational methods for gaining functional insights from sequence data are based on the simple, but powerful, observation that functionally important nucleotides tend to remain unchanged over evolutionary time, because mutations at these sites generally reduce fitness and are therefore eliminated by natural selection 7 - 15 . (biorxiv.org)

Reduced-representation bisulfite sequencing was performed on both F0 and F1 cerebral cortex and F1 hippocampus to identify genome-wide changes in DNA methylation for direct and developmental exposure, respectively. (biomedcentral.com)

Most cancers exhibit reduced methylation of repetitive sequences such as Long INterspersed Element-1 (LINE-1) and Alu elements. (biomedcentral.com)
Etiologic studies of cancer increasingly use molecular features such as gene expression, DNA methylation and sequence mutation to subclassify the cancer type. (biomedcentral.com)

We find that this type of non-canonical fork convergence in fission yeast is prone to trigger deletions between repetitive DNA sequences via a mechanism we call Inter-Fork Strand Annealing (IFSA) that depends on the recombination proteins Rad52, Exo1 and Mus81, and is countered by the FANCM-related DNA helicase Fml1. (elifesciences.org)
The predicted gene products with sequence similarity amongst the eiAU and SSL 2009a phage genomes include things like the putative small tail proteins tail tape mea certain, major tail proteins, major capsid proteins, head morphogenesis, phage terminase tiny subunit, and the phage terminase large subunit. (hivprotease-signal.com)
Interestingly, other struc tural proteins which includes the host specificity proteins, the tail assembly proteins, and specifically the tail fiber baseplate protein which has become suggested for exclusion in any sequence based mostly phage taxonomy scheme are certainly not shared in between the 2 genomes. (hivprotease-signal.com)
The amino acid sequences of one of many conserved structural pro teins likewise as among the non structural proteins have been selected for phylogenetic evaluation. (hivprotease-signal.com)
The most prominent and familiar aspects of the genome are the regions that code for proteins. (cilecenter.org)
Here, we study a collection of 75 CLIP-Seq experiments mapping the RNA binding sites for a diverse set of 51 human proteins to explore the role of TEs in post-transcriptional regulation of human mRNAs and lncRNAs via RNA-protein interactions. (biomedcentral.com)
We detect widespread interactions between RNA binding proteins (RBPs) and many families of TE-derived sequence in the CLIP-Seq data. (biomedcentral.com)
In the case of the infectious cycle, viruses reach target lymphocytes by cell-to-cell transfer and establish by entry of viral single-stranded RNA, reverse transcription of the viral RNA, integration as a provirus into the host genome, expression of viral proteins, and budding of new virions. (biomedcentral.com)
While many DNA sequences are not used to produce proteins, specific functions have been found for much of this supposed "junk. (drwile.com)
Pachytene piRNAs have been proposed to either regulate mRNAs 10 , 11 , 12 , 13 , 14 or stabilize PIWI proteins for a function that does not require piRNA-guided sequence specificity 15 . (gokcumenlab.org)

Long and Short Interspersed Elements (LINES and SINES) account for 21% and 13% of the genome, respectively. (cilecenter.org)
Most other SINEs are derived from tRNAs including the MIRs (mammalian-wide interspersed repeats). (lookformedical.com)

Sequencing genomes of other species facilitates and extends these goals by revealing general principles of biology. (cilecenter.org)
This annotation step is traditionally performed using alignment based methods, either in a de novo approach or by aligning the genome sequence to a species specific set of repetitive sequences. (biomedcentral.com)
The study highlighted several mechanisms underlying species diversification, including selection acting on existing standing variation, high rates of gene duplication, novel microRNAs and rapid sequence divergence in otherwise conserved non-coding elements. (biomedcentral.com)
The zebra finch MHC differs markedly from that of the chicken, the only other bird species with a complete genome sequence. (biomedcentral.com)

McClintock's work set the stage for the discovery of repeated sequences because transposition, centromere structure, and telomere structure are all possible through repetitive elements, yet this was not fully understood at the time. (wikipedia.org)
ALT is a recombination-based mechanism where one telomere uses other chromosomal, or extrachromosomal, telomeric DNA sequences as a template for telomere elongation. (oncotarget.com)

Introns sequences account for about 30% of the genome. (blogspot.com)

The FANTOM Consortium The transcriptional landscape of the mammalian genome. (nature.com)
Hastings, M. L., Ingle, H. A., Lazar, M. A. & Munroe, S. H. Post-transcriptional regulation of thyroid hormone receptor expression by cis-acting sequences and a naturally occurring antisense RNA. (nature.com)
Transposable elements (TEs) have significantly influenced the evolution of transcriptional regulatory networks in the human genome. (biomedcentral.com)

Many repeat sequences are likely to be non-functional, decaying remnants of Transposable elements, these have been labelled "junk" or "selfish" DNA. (wikipedia.org)
Most of these sequences qualify as junk but they are littered with defective transposable elements that are already included in the calculation of junk DNA. (blogspot.com)
There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. (lookformedical.com)
DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom. (lookformedical.com)
A conserved function of piRNAs across all bilateral animals is to silence sequence-complementary transposable elements (TEs) 1 , 2 , 3 , 4 . (gokcumenlab.org)

All colonies, regardless of the nucleotide sequence of rpsA , were found to be sensitive to all of the drugs tested except for ethambutol. (frontiersin.org)
Several molecular typing methods have been developed to differentiate between M. avium subspecies and strains, such as single nucleotide polymorphism (SNP) typing and mini-satellite sequence analysis-mycobacterial interspersed repetitive-unit variable-number tandem repeats (MIRU-VNTR) ( 5 ). (frontiersin.org)
This combines the basic concepts of Li (Bioinformatics 35:4408-4410, 2019) with current techniques developed for neural machine translation, the attention mechanism, for the task of nucleotide-level annotation of repetitive elements. (biomedcentral.com)
It is also called nucleotide sequence. (lookformedical.com)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. (lookformedical.com)

Repeated sequences (also known as repetitive elements, repeating units or repeats) are short or long patterns of nucleic acids (DNA or RNA) that occur in multiple copies throughout the genome. (wikipedia.org)
The disposition of repetitive elements throughout the genome can consist either in directly adjacent arrays called tandem repeats or in repeats dispersed throughout the genome called interspersed repeats. (wikipedia.org)
Tandem repeats and interspersed repeats are further categorized into subclasses based on the length of the repeated sequence and/or the mode of multiplication. (wikipedia.org)
However, an abundance of neutral repeats can still influence genome evolution as they accumulate over time. (wikipedia.org)
Tandem repeats are repeated sequences which are directly adjacent to each other in the genome. (wikipedia.org)
For minisatellites and microsatellites, the number of times the sequence repeats at a single locus can range from twice to hundreds of times. (wikipedia.org)
Tandem repeats have a wide variety of biological functions in the genome. (wikipedia.org)
In addition to playing an important role in recombination, tandem repeats also play important structural roles in the genome. (wikipedia.org)
So identifying and classifying repeats is an important step in genome annotation. (biomedcentral.com)
For example, in the genomes of rat, mouse and human approximately 40 to 50% of the DNA consists of repeats [ 2 ] and these are divided into many different and partly not easily distinguishable classes. (biomedcentral.com)
The function of repetitive elements has been discussed for a long time [ 3 ] and only recently has the importance of repeats in cellular processes begun to open up [ 4 ]. (biomedcentral.com)
Due to these important roles of repeats, their annotation and classification in assembled eukaryotic genomes is an essential part of a genome project. (biomedcentral.com)
Alphoid repeats or human alpha satellites are a human specific subclass of satellite DNA, which are long sequences of non-coding DNA appearing in tandems, i.e. the repeat instances follow each other without long gaps. (biomedcentral.com)
TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. (lookformedical.com)

Due to its high polymorphism and importance for disease resistance, the major histocompatibility complex (MHC) has been an important focus of many vertebrate genome projects. (biomedcentral.com)
DNA transposons have emerged as indispensible tools for manipulating vertebrate genomes with applications ranging from insertional mutagenesis and transgenesis to gene therapy. (biomedcentral.com)
The Medaka fish ( Orizyas latipes ) Tol2 , belonging to the hAT family of transposons, is the first known naturally occurring active DNA transposon discovered in vertebrate genomes [ 10 ]. (biomedcentral.com)

Recently, Li (Bioinformatics 35:4408-4410, 2019) developed a novel software tool dna-brnn to annotate repetitive sequences using a recurrent neural network trained on sample annotations of repetitive elements. (biomedcentral.com)

Performing genome-wide target profiling on data sets obtained by retrieving chromosomal targeting sequences from individual clones, we have identified several piggyBac and Tol2 hotspots and observed that (4) piggyBac and Tol2 display a clear difference in targeting preferences in the human genome. (biomedcentral.com)

We used the identified variants to construct a CD-1-specific reference genome, which can improve accuracy and reduce bias when aligning genomics data derived from CD-1 mice. (biomedcentral.com)
We validated the CD-1 reference genome and the identified variants by comparative mapping of a wide range of CD-1 genomics datasets between the CD-1 and standard mm10 reference genomes. (biomedcentral.com)
Mapping genomics data to the CD-1 genome rather than mm10 resulted in greater mappability and higher quality of results for all datasets examined. (biomedcentral.com)
It is here suggested that the word "cytogenomics" should rather be used with the goal to paraphrase a new field of research in genomics and diagnostics in human genetics, with an integrative and comprehensive view. (frontiersin.org)
Despite these discoveries, the patterns, rates, and properties of cxSVs have primarily been the focus of cancer genomics and such rearrangements remain largely underappreciated in the human germline. (biomedcentral.com)

Even more-highly repeated sequences - minisatellites, microsatellites and telomeres - may appear as tens or even hundreds of thousands of copies, in aggregate amounting to 15% of the genome. (cilecenter.org)

Heterochromatin is an inert region in the genome and composed of mainly remnants of transposons and repetitive elements. (omicsdi.org)

The sequencing of the zebra finch Taeniopygia guttata genome, in combination with targeted bacterial artificial chromosome (BAC) sequencing, has allowed us to characterize an MHC from a highly divergent and diverse avian lineage, the passerines. (biomedcentral.com)

Today, the structural and regulatory roles of repetitive DNA sequences remain an active area of research. (wikipedia.org)
Our meta-analysis suggests a widespread role for TEs in shaping RNA-protein regulatory networks in the human genome. (biomedcentral.com)
The region of DNA which borders the 3' end of a transcription unit and where a variety of regulatory sequences are located. (lookformedical.com)

Consequently, phenotypic traits - both macroscopic and molecular - depend on a combination of influences from genome sequences, the individual's life history and the epigenetic signals in the fertilized egg. (cilecenter.org)

Identification and analysis of functional elements in 1% of the human genome by the ENCODE pilot project. (nature.com)

Phylogenetic analysis primarily based to the significant terminase subunit amino acid sequence plus the DNA polymerase I amino acid sequence of eiAU reveal that phages eiAU, eiDWS, and eiMSLS were most much like phage that infect other enterobacteria and Sodalis glossinidius. (hivprotease-signal.com)
Examples of methods utilizing such non-radioactive detection methods include DNA sequencing, oligonucleotide probe methods, detection of polymerase-chain-reaction products, immunoassays, and the like. (justia.com)
Highly repeated sequences, 6K-8K base pairs in length, which contain RNA polymerase II promoters. (lookformedical.com)
Highly repeated sequences, 100-300 bases long, which contain RNA polymerase III promoters. (lookformedical.com)

Some repetitive elements are neutral and occur when there is an absence of selection for specific sequences depending on how transposition or crossing over occurs. (wikipedia.org)
Repetitive elements are enriched in the middle of chromosomes as well. (wikipedia.org)
MIRU-VNTR represents one of the most plastic elements within mycobacterial genomes ( 14 , 15 ). (frontiersin.org)
Repetitive elements of an unknown function surprisingly account for the large fractions of our genomes. (cilecenter.org)
Repetitive elements contribute a large part of eukaryotic genomes. (biomedcentral.com)
A recent overview of functions and the evolution of repetitive elements can be found in [ 9 ]. (biomedcentral.com)
While [ 11 ] mainly describes structural properties of two of the four repetitive element classes, we additionally highlight the biological importance and, if possible, function of specific repetitive elements. (biomedcentral.com)
Elements that are transcribed into RNA, reverse-transcribed into DNA and then inserted into a new site in the genome. (lookformedical.com)
Sequence analysis of the orangutan genome revealed that recent proliferative activity of Alu elements has been uncharacteristically quiescent in the Pongo (orangutan) lineage, compared with all previously studied primate genomes. (biomedcentral.com)
This is primarily because Alu elements do not contain coding sequence and the vast majority of insertions are highly similar to each other. (biomedcentral.com)
During his patients of the Pacific Northwest experiments the Afternoon Leslie Spier was the network ' water elements ' to unveil valid beta-cell connections where the times show embryology, measurements and boom. (dali-art.de)

The human genome contains approximately 3.2 billion base pairs, distributed among 22 paired chromosomes, plus two X chromosomes in females and one X and one Y chromosome in males. (cilecenter.org)
The length of the human genome is about 3 billion base pairs. (lookformedical.com)
The sequences are about 300 base pairs long and are repeated several thousand times throughout the genome. (ojp.gov)

Three types of endonucleases , namely ZFNs (zinc finger nucleases), TALENs (transcription activator-like effector nucleases ), and the CRISPR (clustered regularly interspersed short palindromic regions) associated (Cas9) system have been predominantly utilized for gene editing. (ac.ir)
6. Miller J, Mc Lachlan AD, Klug A. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. (ac.ir)
10. Dreier B, Beerli RR, Segal DJ, Flippin JD, Barbas CF. Development of zinc finger domains for recognition of the 5'-ANN-3' family of DNA sequences and their use in the construction of artificial transcription factors. (ac.ir)

Furthermore, Alu represents the most abundant Short INterspersed Element (SINE) repetitive sequence, representing 11% of total human genome [ 26 ]. (biomedcentral.com)

To detect the epigenetic drift of time passing, we determined the genome-wide distributions of mono- and tri-methylated lysine 4 and acetylated and tri-methylated lysine 27 of histone H3 in the livers of healthy 3, 6 and 12 months old C57BL/6 mice. (aging-us.com)

The presence of repeated sequence DNA makes it easier for areas of homology to align, thereby controlling when and where recombination occurs. (wikipedia.org)
Evolutionary turnover may cause inconsistencies between sequence orthology and functional homology that substantially limit this type of analysis. (biorxiv.org)

Organization of highly repeated sequences in mouse main-band DNA. (wikidata.org)
The Alu sequence family (named for the restriction endonuclease cleavage enzyme Alu I) is the most highly repeated interspersed repeat element in humans (over a million copies). (lookformedical.com)

Some of these repeated sequences are necessary for maintaining important genome structures such as telomeres or centromeres. (wikipedia.org)

We used the called variants to construct a strain-specific CD-1 reference genome, which we show can improve mappability and reduce experimental biases from genome-wide sequencing data derived from CD-1 mice. (biomedcentral.com)

In order to clarify the biological and pharmacological mechanisms of O. sinensis , we carried out the genome sequencing of H. sinensis, anamorph of O. sinensis , for the first time, described the annotation and gene expression, and analyzed the complete sequencing data (Li et al. (springeropen.com)

The limitations of current methods are particularly evident in the vast noncoding regions of eukaryotic genomes, which, despite important recent progress 3 - 6 , remain poorly annotated and understood. (biorxiv.org)
Similar to mammalian pachytene piRNAs, most of the piRNAs from adult chicken testes were not derived from repetitive regions nor genic regions (Fig. 1a, iii ). (gokcumenlab.org)

In many applications the independent detection of multiple spatially overlapping analytes in a mixture is required, e.g., single-tube multiplex DNA probe assays, immuno assays, multicolor DNA sequencing methods, and the like. (justia.com)

As their significance was recognized, it was noted that TEs' ability to self-replicate meant that a beneficial functional role was unnecessary to explain their conquest of the genome [ 3 ],[ 4 ]. (biomedcentral.com)
A major strength of these conservation- or constraint-based approaches is that they sidestep thorny questions about the relationship between the out-comes of biochemical experiments and fitness-influencing functional roles 16 - 19 by getting at fitness directly through observations of evolutionary change. (biorxiv.org)

Similar to other retroviruses, BLV integrates into the host genome as a provirus and induces lifelong infection. (biomedcentral.com)

We used multiple genome-wide sequencing data types and previously published CD-1 SNPs to validate our called variants. (biomedcentral.com)
The bioinformatic examination on the genomes of these 3 E. ictaluri specific bacteriophages corroborate pre viously published data that signifies that these bacterio phages are lytic, and lack any mechanism for lysogenic conversion of their host. (hivprotease-signal.com)
Sequence data now flow copiously, creating the challenges of understanding the information that our genomes contain, and applying the data and analysis to improve human welfare. (cilecenter.org)
To accomplish this objective we performed a retrospective analysis of [11C]PBR28 mind image data in non-human primates that were recently published [6]. (globaltechbiz.com)
We have generated fitCons scores for three human cell types based on public data from EN-CODE. (biorxiv.org)

Many repetitive DNA sequences have been linked to human diseases such as Huntington's disease and Friedreich's ataxia. (wikipedia.org)
Overall, repeated sequences are an important area of focus because they can provide insight into human diseases and genome evolution. (wikipedia.org)
Discoveries of deleterious repetitive DNA-related diseases stimulated further interest in this area of study. (wikipedia.org)
Protein oligomerization is key to countless physiological processes, but also to abnormal amyloid conformations implicated in over 25 mortal human diseases. (bvsalud.org)

Genome editing using targetable nucleases is an emerging technology for precise genome modification in many organisms with hight ability and capability. (ac.ir)
The emergence of TALENs in 2010 has enabled the genome modification of non- model organisms, while the emergence of the CRISPR/Cas9 system in 2013 as a revolutionary genome-editing tool has allowed us to anticipate the forthcoming new era of genome editing research. (ac.ir)

Structural variation (SV) influences genome organization and contributes to human disease. (biomedcentral.com)

Preservation of viral genomes in 700-y-old caribou feces from culosis (MTB) has identified mixed infections involving a subarctic ice patch. (cdc.gov)

Due to the staggered DNA cuts of the genome by the L1-derived endonuclease during TPRT, Alu insertions are flanked by short sequences of duplicated host DNA called target site duplications (TSDs), which can be used to identify the insertion event. (biomedcentral.com)

In this study, we sequenced the complete genome of Nautilus pompilius in the hope of providing a critical reference for the evolution of cephalopods. (nature.com)
First, the complete genome sequence of the tubercle bacillus was deciphered. (tuberculosistextbook.com)

Sequences of DNA or RNA that occur in multiple copies. (lookformedical.com)
TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES). (lookformedical.com)
Copies of the LINE 1 (L1) family form about 15% of the human genome. (lookformedical.com)

Although the repetitive DNA sequences were conserved and ubiquitous, their biological role was yet unknown. (wikipedia.org)
To gain deeper insight into the biological and pharmacological mechanisms, we sequenced the genome of H. sinensis . (springeropen.com)

Organization and sequence studies of the 17-piece chicken conalbumin gene. (wikidata.org)

The substantial terminase subunit which is a structural protein is together with the portal protein viewed as essentially the most universally conserved gene sequence in phages, consequently they are good possibilities to assist in phage classification. (hivprotease-signal.com)

Treatment of human immunodeficiency virus (HIV) infection with ZFN-mediated CCR5 gene disruption is one of the indicator examples of the ability of ZFNs in genome editing. (ac.ir)

In con trast, therapeutics based mostly on these newly recognized human host targets will avoid HIV virus from employing the hosts cellular mechanism for its daily life cycle and are insensitive to drug resistance. (hivprotease-signal.com)
A simple transposon is organized by terminal repeat domains (TRDs) embracing a gene encoding a catalytic protein, transposase, required for its relocation in the genome through a "cut-and-paste" mechanism. (biomedcentral.com)

Anatomy21

Organisms21

Diseases7

Chemicals and Drugs46

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes146

Disciplines and Occupations5

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture2

Information Science14

Health Care4

Genomic20

Genes12

Whole genome3

Randomly throughout the genome1

Eukaryotic genome2

Loci7

Mammalian genomes2

Genetic8

RNAs3

Nucleotides5

Identify genome-wide1

Methylation2

Proteins10

SINEs2

Species4

Telomere2

Introns1

Transcriptional3

Transposable5

Nucleotide6

Repeats14

Vertebrate3

Bioinformatics1

Chromosomal1

Genomics5

Minisatellites1

Transposons1

Bacterial1

Regulatory3

Molecular1

ENCODE1

Polymerase4

Elements11

Base pairs3

Transcription3

Abundant1

Epigenetic1

Homology2

Highly repeated2

Centromeres1

Mice1

Annotation1

Regions2

Assays1

Functional2

Retroviruses1

Data5

Diseases4

Organisms2

Contributes1

Viral1

Insertions1

Complete2

Copies3

Biological2

Organization1

Structural1

Immunodeficiency virus1

Mechanism2