• The extracted parameters in terms of scanning speed, size of the imaged volume and achieved resolutions should provide a baseline for future users designing nano-Tomography experiments on the NanoMAX beamline. (lu.se)
  • To understand how this antibody hinders the spike protein, and to gather the information necessary to reproduce it, the team behind the current study used cryo-electron microscopy (cryo-EM) at the University of Washington Arnold and Mabel Beckman cryoEM center and X-ray crystallography performed at ALS beamline 5.0.2. (lbl.gov)
  • In September 2020 he brought approximately 200 crystals containing proteins from the Sars-CoV-2 virus for analysis in the Manacá beamline, which was developed for experiments involving X-ray diffraction crystallography. (lightsources.org)
  • LP3 has been granted beamtime at BioMAX (the X-ray macromolecular crystallography beamline of MAX IV). (lu.se)
  • The development of new methods is central to this work, particularly using emerging technologies in cryo-electron microscopy, nano and coherent X-ray diffraction, and macromolecular design. (iucr.org)
  • Overall, this work demonstrates how the ensemble nature of macromolecular structure, revealed here by multitemperature crystallography, can elucidate allosteric mechanisms and open new doors for long-range control of protein function. (rcsb.org)
  • XtaLAB Synergy-DW VHF systems are configured with a Hybrid Photon Counting (HPC) X-ray detector, which are ideal for macromolecular crystallography experiments because they are photon counting detectors that directly detect X-ray photons without the need for the intermediate step of converting X-ray photons to light with a phosphor or scintillator. (rigaku.com)
  • As a result, the XtaLAB Synergy DW systems offer outstanding performance for macromolecular single crystal X-ray diffraction experiments. (rigaku.com)
  • A collaborative work between MAX IV and Paul Scherrer Institute researchers investigated a setup to conduct serial and time-resolved macromolecular crystallography at MAX IV. (lu.se)
  • With the rise of time-resolved macromolecular crystallography, and to reap the full potential of 4th generation synchrotron source like MAX IV, the team aimed to work out a setup that makes it possible to follow through proteins' processes and dynamics, make it possible for a new chapter of structural biology, including ultra-high-throughput screening, serial crystallography structure determination of true microcrystals, and especially time-resolved MX. (lu.se)
  • Ultra-bright hard x-ray pulses using the self-seeded FEL were applied to the demonstration of serial femtosecond crystallography (SFX) experiments in 2020. (lightsources.org)
  • The newest facility is SwissFEL, a free-electron laser, which utilizes a highly efficient C-band linear accelerator and short-period in-vacuum undulator magnets to produce femtosecond light pulses of highest intensities in the hard x-ray regime. (psi.ch)
  • One source with two high-flux wavelengths is the foundation of the revolutionary XtaLAB Synergy-DW VHF single crystal X-ray diffractometer. (rigaku.com)
  • The XtaLAB Synergy-DW VHF diffractometer is based on the proven, low-maintenance MicroMax-007 HF microfocus rotating anode X-ray generator . (rigaku.com)
  • A modern single crystal X-ray diffractometer for structural analysis of small molecule samples configured with microfocus sealed tube technology and a direct X-ray detection detector. (rigaku.com)
  • It is the perfect diffractometer for a core facility where protein crystallography and small molecule crystallography are both practiced. (rigaku.com)
  • Although high-resolution structural information can be obtained by X-ray crystallography, cryo-electron microscopy, nuclear magnetic resonance, and structure prediction tools, they are often insufficient for grasping the actual states of proteins in solution. (biophysics.org)
  • Weidenthaler, C. An in situ powder diffraction cell for high-pressure hydrogenation experiments using laboratory X-ray diffractometers. (mpg.de)
  • Over the past two decades various new tools have been developed to perform in situ mechanical testing at synchrotrons X-ray and neutron facilities. (psi.ch)
  • We particular applied in situ dynamic light scattering (6) , small-angle X-ray scattering and transmission electron microscopy experiments. (lu.se)
  • Ptychographic X-ray computed tomography is a quantitative three-dimensional imaging technique offered to users of multiple synchrotron radiation sources. (lu.se)
  • Rigaku offers a wide range of solutions for both academic research and industrial applications for any composition and type of structural information obtainable by X-rays: XRD ( X-ray diffraction ) in combination with DSC , CT ( computed tomography ), handheld Raman , SAXS ( small angle X-ray scattering ), WAXS ( wide angle X-ray scattering ), XRR ( X-ray reflectivity ), and XRT ( X-ray topography ). (rigaku.com)
  • Experiments with E. coli bacteria and eukaryotic cells revealed that the new peptide system is even suitable for transporting other peptides and proteins. (eurekalert.org)
  • This method describes the cloning, expression, and purification of recombinant Nsa1 for structural determination by X-ray crystallography and small-angle X-ray scattering (SAXS), and is applicable for the hybrid structural analysis of other proteins containing both ordered and disordered domains. (jove.com)
  • These various studies indicate a widening of the scope of both X-ray and neutron probes in certain circumstances to elucidate the protonation states in proteins. (rcsb.org)
  • We cause the proteins to form into lattices, fire X-rays at them and get information from those X-ray diffraction patterns to see what the molecule looks like. (newscientist.com)
  • When the structure of the interacting proteins is known, either from experiments or from modeling, several docking algorithms are available which attempt to predict the geometry of the complex. (cecam.org)
  • Automatic LCP (lipidic cubic phase) experiments are also possible. (lu.se)
  • Junliang Sun (Peking University, Beijing, China) works mainly on the development of structure characterization methods (electron crystallography and powder X-ray diffraction) and the synthesis of inorganic materials. (iucr.org)
  • Here, biophysical methods and X-ray crystallography were used to identify the real properties and behaviour of the peptides. (eurekalert.org)
  • X-ray diffraction (XRD) and X-ray fluorescence (XRF) are established analytical methods in drug discovery and production quality control. (rigaku.com)
  • Therefore, having a reliable cascade of screening methods to be used as pre-screens prior to labor-intensive X-ray crystallography would be extremely beneficial. (uni-marburg.de)
  • One of the experimental methods that we use is X-ray crystallography," says Higgins. (newscientist.com)
  • Today, there is an evolving need for more imaging methods and for biophysics that cannot be addressed with crystallisation and crystallography. (infn.it)
  • or where current structural information is significantly compromised by damage from conventional X-ray methods ( e.g. redox effects in metalloproteins). (stanford.edu)
  • Hard X-rays and high repetition rates will further enable advanced crystallography methods that exploit diffuse scattering from imperfect crystals, as well as advanced solution scattering and single particle imaging methods to map sample heterogeneity and conformational dynamics in native environments. (stanford.edu)
  • Therefore, new, advanced and reliable methods to prepare, detect and score 3D micro- and nano-sized crystal suspensions, most suitable for X-ray diffraction experiments need to be established in time. (lu.se)
  • The most common strong alternative and complement to experimental methods to methods to obtain protein structures are X-ray crystallography obtain insight in biochemical processes. (lu.se)
  • A method is called theoretical if it obtains its results from calcula- structure and function of metalloproteins with theoretical methods tions rather than from experiments. (lu.se)
  • Recent developments within protein crystallography methods in combination with the opportunities offered by the new upcoming synchrotron sources such as the MAX IV 3 GeV storage ring, make new exciting ways of carrying out research within structural biology possible. (lu.se)
  • New technical solutions for sample handling and presentation, data processing methods and more will be needed for these types of experiments, and these will also be presented and discussed at the workshop. (lu.se)
  • They also conducted high-throughput laboratory screening experiments, structural studies, and molecular dynamics simulations to learn how these potential inhibitors and the enzyme interact. (bnl.gov)
  • Artist's impression of the experiment: When the ultra-bright X-ray flashes (violet) hit the enzyme crystals in the water jet (blue), the recorded diffraction data allow to reconstruct the spatial structure of the enzyme (right). (bionity.com)
  • To unravel the three-dimensional structure of a biomolecule, such as an enzyme, the pulses are used to obtain flash X-ray exposures of tiny crystals grown from that biomolecule. (bionity.com)
  • Room-temperature X-ray crystallography provides unique insights into protein conformational heterogeneity, but obtaining sufficiently large protein crystals is a common hurdle. (iucr.org)
  • Serial synchrotron crystallography (SSX) helps to address this hurdle by allowing the use of many medium- to small-sized crystals. (iucr.org)
  • X-ray crystallography provides structural information that is crucial for fragment optimization, however there are several criteria that must be met for a successful fragment screening including the production of soakable and well-diffracting crystals. (uni-marburg.de)
  • Pink crystals reveal the mechanism of chloride transport over the cell membrane: Using time-resolved serial crystallography, the pink NmHR crystals revealed ion binding sites in the chloride transporter and pumping dynamics after photoactivation. (scitechdaily.com)
  • Our most popular diffractometer for Chemical Crystallography and Mineralogy , configured with either single or dual microfocus sealed tube X-ray sources and an extremely low noise direct X-ray detection detector. (rigaku.com)
  • An entry-level single crystal X-ray diffractometer for Chemical Crystallography configured with microfocus sealed tube technology and a direct X-ray detection detector. (rigaku.com)
  • The scientific possibilities will be highlighted through a series of talks by experts showing the impact of the new type of X-ray sources and microfocus beamlines on research within structural biology. (lu.se)
  • At the LP3 crystallisation and biophysical laboratory, you can carry out a wide variety of robot-assisted crystallisation experiments in nano volumes as well as screen for a large number of buffer conditions to stabilize their sample. (lu.se)
  • Performs automatic imaging of crystallisation experiments at 20 °C using visible and UV light. (lu.se)
  • His work is largely based on diffraction phenomena and combines computational, biochemical and biophysical experiments. (iucr.org)
  • The MYTHEN single-photon-counting silicon microstrip detector has been developed at the Swiss Light Source for time-resolved powder diffraction experiments. (scienceopen.com)
  • Time-resolved hard X-ray spectroscopy with high fidelity, enabled by LCLS-II-HE, will reveal the fine details of functioning biological catalysts (enzymes) and inform the design of artificial catalysts and networks with targeted functionality. (stanford.edu)
  • Photoactive chloride pumping through the cell membrane captured by time-resolved serial crystallography: Chloride ions (green spheres) are transported across the cell membrane by the NmHR chloride pump (pink). (scitechdaily.com)
  • Slower dynamics in the millisecond-range were investigated via time-resolved serial crystallography at SLS while faster, up to picosecond, events were captured at SwissFEL - then both sets of data were put together. (scitechdaily.com)
  • We have piloted data search through heterogeneous data sources from a single interface across samples and experiments for Inner Shell Spectroscopy (ISS) and X-ray Powder Diffraction (XPD). (bnl.gov)
  • It is an electron storage ring that runs at a constant beam current (Top-up) and delivers synchrotron radiation (X-rays) simultaneous to 16 experiments, each highly specialized for specific applications (Tomography, protein crystallography, lithography, etc. (psi.ch)
  • Test experiments have been conducted at two photon energies, 9.7 keV and 12.7 keV. (lightsources.org)
  • In addition to materials characterization, a Core Biology Facility that serves multiple users is housed within the department for performing bioassays, cell and tissue culture work, and molecular biology experiments. (ndsu.edu)
  • We use molecular modeling to obtain an estimate of a protein's structure in the short term, and try to solve the structure using x-ray crystallography in the long term. (abo.fi)
  • These interpretations lead to predictions about molecular interactions and suggest experiments that can help to verify the predictions or to suggest alterations in the model structure. (abo.fi)
  • The experiment shows that the setup, based on JUNGFRAU detector and Jungfraujoch data-acquisition system, can provide a molecular moving picture of up to 500 microseconds in resolution of protein dynamics - providing ten times finer details than the previously available method. (lu.se)
  • The use of molecular mechanics calculations to supplement experimental data in standard X-ray crystallography and NMR refinements is discussed and it is shown that structures can be locally improved by the use of quantum chemical calculations. (lu.se)
  • Recent studies by x-ray crystallography, NMR, and molecular sim- present). (lu.se)
  • Recent years have seen the rapid development and expansion of electron crystallography, much of it highlighted at the IUCr Congress in Prague last year. (iucr.org)
  • Other teams ran laboratory experiments testing those molecules' ability to inhibit the virus. (bnl.gov)
  • The Materials Characterization Laboratory houses the departmental X-ray crystallography facilities and a brand new Analytical Ultracentrifuge. (ndsu.edu)
  • metal site is extended X-ray absorption fine structure (EXAFS). (lu.se)
  • The protonation states of aspartic acids and glutamic acids can be determined (on the basis of convincing evidence) even to the modest resolution of 1.20 A as exemplified by our X-ray crystal structure refinements of Ni and Mn concanavalin A and also as indicated in the 1.26 A structure of thrombin, both of which are reported here. (rcsb.org)
  • The pioneering work not only demonstrates that the new research facility can speed up experiments by more than an order of magnitude, it also reveals a previously unknown structure of an enzyme responsible for antibiotics resistance. (bionity.com)
  • X-ray crystallography offers detailed insights into protein structure. (iucr.org)
  • Using X-ray crystallography to view Anf3's structure at an atomic resolution, the team saw that it was similar to a family of oxygen-reducing enzymes. (asbmb.org)
  • Element analysis of the main components and trace elements can be performed with X-ray fluorescence (XRF), and crystal structure determination and phase analysis and quantification are studied with X-ray diffraction ( XRD ) in your lab or fab. (rigaku.com)
  • In such structural studies, Beese uses X-ray crystallography, a technique that involves shining X-rays through a crystallized protein and deducing the molecule?s structure from the resulting pattern of diffracted spots. (scienceblog.com)
  • Birney says that using X-ray crystallography to map the structure of a protein is expensive and time-consuming. (newscientist.com)
  • The X-ray structure of the casposase bound to DNA representing the product of integration reveals a tetramer with target DNA bound snugly between two dimers in which single-stranded casposon end binding resembles that of spacer 3'-overhangs. (elifesciences.org)
  • In facts, often limitations in the availability of high-quality samples for X-ray crystallography call for the use of lower-resolution techniques like cryo-EM, where a compelling reconstruction of the structure of the complex requires the combined use of advanced computational techniques. (cecam.org)
  • X-ray crystallography has been employed to determine the structure of one of the major products entitled pseudoclovene A and a synthesis of this olefin has been achieved. (gla.ac.uk)
  • Thus, using computational approaches we interpret experiments in the light of the three-dimensional structure of the receptor protein. (abo.fi)
  • The structure and absolute configuration have been confirmed with single crystal X-ray crystallography. (who.int)
  • Spectacular performance combined with dual wavelength versatility, provides the perfect answer for high-capacity Chemical Crystallography labs or for X-ray facilities that support Chemical Crystallography and Protein Crystallography . (rigaku.com)
  • A dual-wavelength, extremely high-flux, rotating anode X-ray diffractometer specifically configured for measuring difficult samples as well as providing versatility through multiple wavelengths. (rigaku.com)
  • Recent technological breakthroughs, such as ultra-fast pulsed lasers, high-performance computing and crystallography of macromolecules, helped to improve the experimental tools of physicists, biologists, and chemists working, in particular, in the area of chemical and biological physics. (cecam.org)
  • Its dependence on the coherent fraction of the available X-ray beam makes it perfectly suited to diffraction-limited storage rings. (lu.se)
  • By using coherent X-ray diffraction techniques, scientists can measure the exterior shape of and strain in nanocrystalline materials with a high degree of precision. (anl.gov)
  • LCLS-II-HE will image dynamics at the atomic scale via hard X-ray scattering and coherent diffractive imaging (CDI) to reveal the coupled behavior of electrons and atoms with unprecedented clarity. (stanford.edu)
  • Our most popular diffractometer for Protein Crystallography , configured with a high-flux rotating anode X-ray source and an extremely low noise direct X-ray detection detector. (rigaku.com)
  • A curved single crystal X-ray diffraction detector based on direct X-ray detection technology with a higher 2θ range compared to a flat detector. (rigaku.com)
  • A unique curved single crystal X-ray diffraction detector based on direct X-ray detection technology with the highest 2θ range at a single position available for the home lab. (rigaku.com)
  • Extremely low noise, air-cooled X-ray detector based on direct X-ray detection technology. (rigaku.com)
  • X-ray crystallography allows the detection of specific binders that may be too weak binders to be detected by SPR and even by NMR but can still provide valid structural information to support the search for appropriate starting points in lead discovery. (uni-marburg.de)
  • Usually, when people look at microcrystals using X-ray diffraction, they're glued onto a sample holder, which causes a distortion," said Argonne distinguished fellow Linda Young, a corresponding author on the study. (anl.gov)
  • The unique LCLS-II-HE capability for simultaneous delivery of hard and soft X-ray pulses opens the possibility to follow chemical dynamics (via spectroscopy) concurrent with structural dynamics (substrate scattering) during heterogeneous catalysis. (stanford.edu)
  • Faster, accurate data collection due to high-speed kappa goniometer, high-flux rotating anode X-ray source, fast, low-noise X-ray detector, and highly optimized instrument control software. (rigaku.com)
  • Traditionally, X-ray crystallography was dominating the interaction of large facilities with life sciences. (infn.it)
  • Structural studies using, for example, x-ray crystallography revealed how the candidate molecules fit together with the virus enzyme. (bnl.gov)
  • The concept will be rapidly prototyped for a proof-of-concept experiment, for feasibility evaluation, and for providing a preliminary design and demonstration plan for Phase II, which will involve development, building, and testing of a brass-board model of the optical security system. (nsf.gov)
  • cosmic ray and particle detectors and a gamma ray burst detectors. (wikipedia.org)
  • Improve your ability to investigate small samples due to the increased flux from the rotating anode X-ray source as well as the extreme low noise of the HyPix X-ray detectors. (rigaku.com)
  • Additionally, HPC X-ray detectors have a top-hat point spread function of a single pixel. (rigaku.com)
  • Many aspects of discovery, design, development and formulation processes require structural information acquired by X-ray diffraction techniques. (rigaku.com)
  • A bespoke, extremely high-flux diffractometer with custom enclosure and the flexibility to utilize both ports of the rotating anode X-ray source. (rigaku.com)
  • An extremely high-flux rotating anode X-ray diffractometer specifically configured for measuring difficult samples. (rigaku.com)
  • It combines the increased flux of a rotating anode X-ray source with the flexibility of two different wavelengths, making it ideal for laboratories exploring a wide range of research interests. (rigaku.com)
  • The PhotonJet-DW X-ray source applies the lessons learned over the development and lifetime of the MicroMax™-007 rotating anode to produce a new generation, high-performance rotating anode source. (rigaku.com)
  • The key data challenges of complexity, velocity, and volume require a new data and computing infrastructure, including curation, detailed recording of processes acting on data, and advanced computing discovery techniques integrated into computational workflows to enable the autonomous experiments in the future. (bnl.gov)
  • As announced in the January issue of the journal, Professor Xiaodong Zou (Stockholm University, Sweden) has been appointed as Main Editor of the electron crystallography section. (iucr.org)
  • Journal of Applied Crystallography 2015 , 48 , 79-84. (mpg.de)
  • We have consistently improved the spectral purity and peak of the self-seeded FEL using a laser heater and optimized crystal conditions over a hard x-ray range from 3.5 keV to 14.6 keV. (lightsources.org)
  • I think we can be confident that there are new experiments and new insights coming through due to AlphaFold, which will impact 'how does this particular parasite work' or 'why does this particular disease happen in humans', for example. (newscientist.com)
  • To further increase beamtime, the PAL-XFEL has plans for 24-hour operation and simultaneous operation of hard and soft X-ray beamlines in the near future. (lightsources.org)
  • The beams are directed into beamlines to support a wide range of scientific techniques, including protein crystallography. (lbl.gov)
  • For an accurate description of an experiment all modes which can be probed experimentally, e.g., via spectroscopic techniques, have to be treated explicitly. (cecam.org)