Many of these RNA metabolism proteins are represented in the components of the multi-enzyme RNA degradosome of Escherichia coli, which is constituted by four basic components: the hydrolytic endo-ribonuclease RNase E, the phosphorolytic exo-ribonuclease PNPase, the ATP-dependent RNA helicase (RhIB) and a glycolytic enzyme enolase. (wikipedia.org)
The RNA degradosome was discovered in two different laboratories while they were working on the purification and characterization of E. coli, RNase E and the factors that could have an influence on the activity of the RNA-degrading enzymes, concretely, PNPase. (wikipedia.org)
PNPase: a phosphorolytic exo-ribonuclease that degrades RNA. (wikipedia.org)
In Escherichia coli, a proportion of the PNPase is recruited into a multi-enzyme assembly, known as the RNA degradosome, through an interaction with the scaffolding domain of the endoribonuclease RNase E. Here, we report crystal structures of E. coli PNPase complexed with the recognition site from RNase E and with manganese in the presence or in the absence of modified RNA. (port.ac.uk)
The homotrimeric PNPase engages RNase E on the periphery of its ring-like architecture through a pseudo-continuous anti-parallel beta-sheet. (port.ac.uk)
Manganese can substitute for magnesium as an essential co-factor for PNPase catalysis, and our crystal structure of the enzyme in complex with manganese suggests how the metal is positioned to stabilise the transition state. (port.ac.uk)
Polynucleotide1
It contains the proteins RNA helicase B, RNase E and Polynucleotide phosphorylase. (wikipedia.org)
Coli1
In E. coli, RNase E is located in the cytoplasmatic membrane and can be observed by fluorescence microscopy. (wikipedia.org)
RRNA1
Occasionally, copurification of rRNA with degradosome is appreciated, which suggests that the complex may take part in rRNA and mRNA degradation. (wikipedia.org)
Recognition1
All organisms have various tools for RNA degradation, for instance ribonucleases, helicases, 3'-end nucleotidyltransferases (which add tails to transcripts), 5'-end capping and decapping enzymes and assorted RNA-binding proteins that help to model RNA for presentation as substrate or for recognition. (wikipedia.org)
Proteins2
Frequently, these proteins associate into stable complexes in which their activities are coordinate or cooperative. (wikipedia.org)
Ppk (polyphosphate kinase) is another constituent that has been reported to be part of the complex, the same as RNA chaperone Hfq, PAP (prostatic acid phosphatase), other kinds of chaperones and ribosomal proteins. (wikipedia.org)
Transcripts1
We previously demonstrated that TGF-β upregulates activation-induced cytidine deaminase (AID) expression in hepatocytes, which in turn downregulates HBV transcripts by recruiting the RNA exosome complex. (fujita-hu.ac.jp)
Polymerase1
EXOSC2 is a component of the RNA exosome, and here, LC-MS/MS analysis of protein pulldowns demonstrated interaction between the SARS-CoV-2 RNA polymerase and most of the human RNA exosome components. (ox.ac.uk)
DEGRADATION2
An intracellular ribonucleolytic protein complex that participates in POSTRANSCRIPTIONAL RNA PROCESSING and RNA DEGRADATION. (bvsalud.org)
The degradosome is a multiprotein complex present in most bacteria that is involved in the processing of ribosomal RNA and the degradation of messenger RNA and is regulated by Non-coding RNA. (wikipedia.org)
DEHYDROGENASE1
PYRUVATE DEHYDROGENASE ( LIPOAMIDE )- PHOSPHATASE catalyzes reactivation of the complex. (nih.gov)
Human1
A similar interaction pattern occurs in the structurally homologous human exosome between the Rrp45 and Rrp46 subunits. (port.ac.uk)
Protein complex1
The multi-protein complex also serves as a machine for processing structured RNA precursors in the course of their maturation. (wikipedia.org)
Degradosome1
Additional alternate degradosome components during stationary phase include Rnr (RNase R) and the putative RNA helicase HrpA. (wikipedia.org)
Components1
So the structure is like a molecular domain where RNA can interact as a substrate with each of the components and when this happens, it is really difficult for RNA to scape from the complex. (wikipedia.org)
Formation2
A multienzyme complex responsible for the formation of ACETYL COENZYME A from pyruvate. (nih.gov)
These observations suggest the PDX1:SND1 complex formation is critical for controlling a subset of genes important for ß cell function and is targeted in diabetes pathogenesis. (bvsalud.org)